defects in granulocyte function in various chromosome abnormalities (down's-, edwards'-, cri-du-chat syndrome).in five infants with autosomal aberrations and diminished resistance to infection (in spite of intact humoral and cellular immune mechanisms) several granulocyte functions (chemotaxis, phagocytosis, intracellular killing and metabolism of killing) were measured. a serum-dependent or a cell-dependent disturbance of phagocytosis of candida albicans was found in two infants with cat-cry syndrome and one with trisomy 18. in one of these children there was an additional serum dependent defect of the ...1976176505
[obtaining the beta-globulin fractions from swine and cattle sera and a study of their immunological activity].the rivanol precipitation was used to obtain beta-globulin fractions from specific swine sera against edema disease, paratyph and aujeszky's disease as well as from normal ovine and swine sera. agar electrophoresis revealed that the preparations produced contained beta-globulin (86 per cent), gamma-globulin (5 per cent), and alpha2-globulin (9 per cent). the beta-globulin preparations were studied for the presence of antibodies against e. coli, salmonellae, staphylococci, myxovirus parainfluenza ...1976176771
effect of infection with enveloped viruses on nucleotide metabolism. 1976176807
[synthesis of virus-specific macromolecules and virion maturation in chick embryo cells infected with sendai virus].synthesis and accumulation of virus-specific rna, proteins and intracellular nucleocapsids are observed in sendai virus-infected chick embryo cells for approximately 2 days postinfection. however, only an insignificant part of these products leaves the cell as a result of maturation of virus particles. it is suggested that the cause of the ineffective maturation of virions in the system under study may be in the observed instability of one of virus structural proteins in the infected cells, m pr ...1976176819
[recognition of influenza and other acute respiratory virus infections and their combinations by means of immunofluorescence].the results of postmortem examinations performed in 237 fatal cases of influenza and other acute respiratory diseases are presented. in smears of organs and tissue culture cells infected with these materials and heart blood the immunofluorescent procedure detected influenza virus antigens in 67% of the cases, antigens of other respiratory viruses in 53% including 18-19% of mixed infections. parallel immunofluorescent studies in living patients and isolation of influenza virus and adenoviruses in ...1975176823
gangliosides of hepatoma 27, normal and regenerating rat liver.the highly malignant rat hepatoma 27 was found to have increased amounts of lipid-bound sialic acid as compared with normal liver whereas in regenerating liver the lipid-bound sialic acid level was reduced. in contrast to the liver the hepatoma contained higher amounts of disialogangliosides and no trisialogangliosides, which are abundant in the liver. the main disialoganglioside of the hepatoma had no analogue among the liver gangliosides and was identified as gal-galnac(acneu-acneu)-glc-cer (g ...1976177287
horse-liver alcohol dehydrogenase and pseudomonas testosteroni 3(17)beta-hydroxysteroid dehydrogenase transfer epimeric hydrogens from nadh to 17beta-hydroxy-5alpha-androstan-3-one. an exception to one of the alworth-bentley the reduction of 17beta-hydroxy-5alpha-androstan-3-one to the 3beta-alcohol, horse liver alcohol dehydrogenase utilizes the 4-pro-r hydrogen of nadh whereas the 3(17)beta-hydroxysteroid dehydrogenase of pseudomonas testosteroni utulized the 4-pro-s hydrogen. these observations provide an exception to the rule proposed by alworth and bentley that with regard to the paired methylene hydrogens at c-4 of nadh and nadph "the stereospecificity of a particular reaction is fixed and does not vary wit ...1976177288
evaluation of the antilipolytic action of clomiphene in vitro.clomiphene (10(-3) - 10(-2) m) in a dose-dependent manner inhibited the lypolytic response of isolated rat epididymal adipose tissue and fat cells to epinephrine, acth, and dibutyryl-cyclic amp. furthermore, it reduced the non-hormonally stimulated activity of a crude preparation of lipase from epididymal adipose tissue. the accumulation of cyclic amp produced by epinephrine in fat cells was not prevented by clomiphene at a concentration causing antilipolytic activity. it is concluded from these ...1976177345
hepatoma induced by thorium dioxide.a 74-year-old man complained of anorexia and weight loss. twenty-six years earlier he had received an injection of thorotrast. a needle biopsy of the liver showed thorium dioxide granules and periportal fibrosis. on laparotomy, a hepatoma of the left lobe of the liver was discovered. hepatic malignancy should be suspected in any patient with abnormal results of liver function tests, particularly an elevated level of alkaline phosphatase, who previously has had an injection of thorotrast.1976178054
pituitary cyclic amp production and mechanism of luteinizing hormone release. 1975178535
null carcinogenic effect of large doses of nitrosoproline and nitrosohydroxyproline in wistar rats. 1976178581
immunology of herpes simplex virus in fection.the accompanying inflammation seen in ocular hsv infection-the result of interactions between viruses and the immune response-can be both beneficial and potentially harmful to the host. an understanding of the interaction of virus-immune cells is just evolving from current advances in basic research. based on our studies on hsv keratitis [11], the control of ocular hsv infection appears to involve an early inflammatory phase with macrophage reactivity and elaboration of mif. transient virus-spec ...1975178622
lipids of klebsiella pneumoniae: the presence of phosphatidyl choline in succinate-grown cells.the carbon and energy source for aerobically grown cultures of klebsiella pneumoniae profoundly influenced the total lipid content and phosphatide composition. glucose-grown cells contained 13% lipid, 56% of which was phospholipids. succinate-grown cells contained 8% lipid, 66% of which was phospholipids. the predominant phosphatides of glucose-grown cells were phosphatidyl ethanolamine, 82%; phosphatidyl glycerol, 4.5%; phosphatidic acid, 5%; cardiolipin, 6.5%; phosphatidyl serine; and trace am ...1976178973
the influence of some dietary factors and/or treadmill exercise on rat and chicken tissue lipids and serum lipoproteins.four different experiments, one with cockerels and three with rats, are described which relate to the effect of dietary factors and/or exercise on serum and liver lipids as well as on serum lipoproteins. several classes of lipoproteins were isolated by preparative ultracentrifugation, and their purity assessed by gel electrophoresis. the lipid composition and protein content of serum lipoproteins and tissue were also determined. dietary cholesterol produced an enormous increase (200 fold) in ser ...1976179301
optic nerve manifestations of human congenital cytomegalovirus infection.a latin american male and a white female infant who had a cytomegalovirus infection on the first day of life had unilateral optic nerve hypoplasia. a white male infant who had cytomegalovirus isolated at 5 weeks of age had a unilateral partial coloboma of the optic nerve. a 4-month-old black infant with cytomegalovirus infection diagnosed at 2 days of age had a unilateral complete coloboma of the optic nerve associated with microphthalmia. optic nerve involvement was an important manifestation o ...1976179325
detection of type-specific antibody to herpes simplex virus type 1 and 2 in human sera by complement-fixation tests.type-specific antigens for herpes simplex virus type 1 and 2 were prepared by rigorous absorption of cell extracts with heterotypic immune sera. type-specificity was demonstrated by immunodiffusion and complement-fixation tests against immune sera prepared in rabbits. specific type 1 complement-fixing reactivity was detected in eleven of fifteen sera from roman catholic nuns and in two convalescent sera from patients with recurrent herpes labialis; these sera had been previously shown to contain ...1976179502
properties of the low-temperature photosystem i primary reaction in the p-700-chlorophyll alpha-protein.the photosystem i primary reaction, as measured by electron paramagnetic resonance changes of p-700 and a bound iron-sulfur center, has been studied at 15 degrees k in p-700-chlorophyll alpha-protein complexes isolated from a blue-green alga. one complex, prepared with sodium dodecyl sulfate shows p-700 photooxidation only at 300 degrees k, whereas a second complex, prepared with triton x-100, is photochemically active at 15 degrees k as well as at 300 degrees k. analysis of these two preparatio ...1976179589
daily variation in concentration of plasma corticosteroid in hypophysectomized rats.a daily variation in the concentration of plasma corticosteroid hormone is present in hypophysectomized rats implanted with pellets of adrenocorticotropic hormone (acth) and thyroxine. although acth is necessary to maintain a functional adrenal, and thyroxine is required to permit the expression of the plasma corticosteroid rhythm, a normal daily variation in plasma corticosteroid concentration does not appear to depend on the periodic release of pituitary hormones.1976179796
steroid production by quartered and capsular rat adrenal gland in response to haemorrhage.60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ...1975179800
[changes in the functional state and structure of the adrenals in cc14 poisoning].introduction of cci4 in doses of 0.1 and 0.2 ml/100 g to albino rats reduced the steroid production generated by adrenal sections. in 24 and 48 hours after poisoning with a small dose of cci4 (0.1 ml/100 g) there could be seen an increased concentration of 11-oxycorticosteroids in the plasma. morphological signs of a damage suffered by the gland were observed only in poisoning with a large dose of cci4 (0.2 ml/100 g).1975179852
nad+-malic enzyme. regulatory properties of the enzyme from ascaris suum.the regulatory properties of the nad-dependent malic enzyme from the mitochondria of ascaris suum have been studied. the malate saturation curve exhibits sigmoidicity and the degree of this sigmoidicity increases as the ph is increased. fumarate was the only compound tested that stimulated the enzyme activity, whereas oxalacetate was the most powerful inhibitor. activation by low levels of fumarate was found to be competitive with malate. it is proposed that this stimulation has physiological si ...1976180002
stimulation of ornithine decarboxylase synthesis and its control by polyamines in regenerating rat liver and cultured rat hepatoma cells.ornithine decarboxylase has been induced in log phase hepatoma cells grown in suspension culture. induction with n6, o2'-dibutyryl cyclic adenosine 3':5'-monophosphate produced a 4-fold increase in enzyme activity by 3 hours which was followed by a return to base levels by 6 hours. induction with dexamethasone, a potent synthetic glucocorticoid, exhibited a slow steady rate of increase in enzyme activity, reaching a plateau level of approximately 5- to 6-fold stimulation by about 12 hours. indu ...1976180026
experimental encephalitis caused by herpes simplex virus: comparison of treatment with tilorone hydrochloride and phosphonoacetic acid.a mouse model of encephalitis caused by herpes simplex virus was used to compare the antiviral activity of tilorone hydrochloride with that of phosphonoacetic acid. these compounds were also administered simultaneously to determine whether the combination had a synergistic effect. rates of survival and concentrations of virus in brain were used as criteria for judging the effectiveness of treatment. the fatal course of encephalitis was not altered by any treatment protocols in which tilorone hyd ...1976180204
the cleavage of polyoma virus dna by restriction enzymes kpni and psti.the action of two restriction endonucleases on polyoma virus dna has been examined and the sites at which they cleave the dna located. one of the enzymes, kpni from klebsiella pneumoniae ok8, cleaves polyoma dna twice at about 11-6 and 59-2% from the ecori site. the other enzyme, psti from providencia stuartii 164, cleaves polyoma dna five times at about 14-8, 16-5, 32-6, 50-3 and 80-0% from the ecori site. some of the cleavages produced by these enzymes alone, or in conjunction with other endon ...1976180247
[polyphosphate biosynthesis in rhodospirillum rubrum chromatophores].the chromatophores of rhodospirillum rubrum were found to synthesize in the light not only atp and pyrophosphate but also high molecular weight polyphosphates. biosynthesis of all studied compounds was inhibited by antimycin a, an inhibitor of the electron-transport photosynthetic chain. synthesis of high molecular weight polyphosphates is stimulated, while that of pyrophosphate is inhibited, in the conditions providing intensive synthesis of atp (in the presence of adp and in the absence of oli ...1976180387
initiation sites of rous sarcoma virus rna-directed dna synthesis in vitro.rous sarcoma virus dna synthesis in disrupted virions is initiated mainly at a site about 200 nucleotides or less from the 5' terminus, but other initiation sites throughtout the rna seem to be used as well. no aug triplet occurs within a 5' terminal segment of about 25 nucleotides.1976180433
experimental alterations in cyclic adenosine monophosphate concentrations in the cat basilar artery.levels of cyclic adenosine monophosphate (amp) in the basilar artery and in circulating blood of cats were determined after the production of spasm by topical application of blood to the vessel and following treatment with agents known to alter cyclic amp. isoproterenol, known to stimulate adenyl cyclase, and aminophylline, a phosphodiesterase inhibitor, were studied alone and in combination. cyclic amp of the basilar artery fell from a mean control value of 43 to 26 pmoles per milligram of prot ...1976180456
ammonium sulfate activation of phosphodiesterase in homogenates of larvae of the european corn borer.european corn borer phosphodiesterase is highly activated by (nh4)2so4 and moderately activated by nh4c1 (ph 7.6, 33 degrees). vertebrate and crayfish diesterases, on the other hand, are inhibited by (nh4)2so4. it is likely that (nh4)2so4 causes some configurational change in the european corn borer phosphodiesterase molecule which results in the exposure of more active sites and hence greater enzyme activity. in in vitro tests caffeine (0.008 m) and theophylline (0.008 m) inhibit phosphodiester ...1976180541
[epidemiological aspects of cytomegalovirus infections]. 1975180573
isolation of pseudomonas aeruginosa and other bacterial species from ornamental aquarium plants.ornamental aquarium plants were demonstrated to carry as many as 10(8) viable mesophilic bacteria per g. gram-negative organisms predominated among the 19 genera of bacteria identified. pseudomonas aeruginosa was readily isolated from 53% of the samples tested.1976180888
stimulation of glycogenolysis and gluconeogenesis by epinephrine independent of its beta-adrenergic function in perfused rat liver. 1976181007
fine structural analysis of rabbit synovial cells. ii. fine structure and rosette-forming cells of explant and monolayer cultures.the fine structure and plasma membrane receptors of explant and monolayer cells of normal rabbit synovium were studied. in explants about 10% of the cells were round and formed rosettes with igc and c3 markers, whereas the remaining cells were stellate, resembled young fibroblasts, and had no receptors for igg and complement. monolayer cells looked like fibroblasts, produced fine extracellular fibrils and hyaluronate, and formed no rosettes. thus early cultures contain both macrophages and fibro ...1976181009
in vivo activation by polymyxin b of phospholipase from pseudomonas aeruginosa and escherichia coli. 1976181357
fetal lung maturation iv: the release of phosphatidic acid phosphohydrolase and phospholipids into the human amniotic fluid.the relationship between surface active material and phosphatidic acid phosphohydrolase (papase) activity was examined in human amniotic fluid after centrifugation at 105,000 x g for one hour. the distribution of papase activity between the pellet and supernatant fractions was similar to that of phospholipids in the amniotic fluid. the majority of the papase activity was found to be associated with the pellet fraction. similarly, 82% of the phospholipid was also present in this fraction. of the ...1976181724
a protein-binding assay for direct determination of adenosine 3',5'-monophosphate in amniotic fluid, cerebrospinal fluid, plasma, and adenosine 3',5'-monophosphate (cyclic amp)-binding protein was isolated from bovine skeletal muscle. this preparation showed maximum binding capacity for cyclic amp at the physiological ph of amniotic fluid, cerebrospinal fluid, and plasma and had a high association constant of 1.4x10(9) 1/mol. this preparation of binding protein, together with albumin and edta in the assay buffer, gave a sensitive and specific competitive protein-binding assay that permitted direct determination of cyclic am ...1976181838
[application of scanning electron microscopy in the evaluation of iron content of human synovial membrane]. 1976181851
enzootic with ectromelia symptomatology in sprague-dowley rats. 1976181904
[adsorption of enteroviruses on nitrate cellulose membranes: influence of mineral salts, detergents and calf serum (author's transl)].the filtration through cellulose nitrate membranes of a poliovirus type ii suspension in distilled water alone or with added mineral salts (nacl, mgcl2, alcl3) results in adsorption of the virus. the maximal adsorption is obtained with nacl. a detergent in the virus suspension or a pretreatment of the membranes with calf serum prevents this adsorption. elution of the adsorbed enteroviruses is achieved with alcaline and proteinaceous buffer.1976182051
properties and partial purification of an oestrogen receptor from neonatal rat brain. 1976182324
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
preliminary results with an oral application of killed purtussis bacteria in newborn infants.newborn babies have been vaccinated orally on five consecutive days, with 5 droplets of a vaccine containing 10(11) killed bordetella pertussis. eight out of twelve subjects showed antibody titres varying between 1:20 and 1:80.1976182603
the stimulatory effect of hypothalamic and mesencephalic tissue on the interrenal of tadpoles of xenopus laevis daudin. 1976182611
positive differential heterophile antibody test. persistence in a symptomatic patient.a patient who had documented mononucleosis in june 1971 has been persistently symptomatic since that time, with easy fatigability and weight loss. he has also had a positive differential heterophile antibody test for mononucleosis for 14 months. although no evidence for active estein-barr virus (ebv) infection was discovered, the possibility of a chronic ebv infection is discussed.1976183027
letter: isolation and characterisation of equine herpes virus type 1. 1976183339
polynucleotide sequences common to the genomes of simian virus 40 and the human papovaviruses jc and bk. 1976183358
persistence of virus-specific igm and clinical recovery after japanese encephalitis.we have searched for evidence of a chronic japanese encephalitis virus (jev) infection in six thai patients convalescing from acute japanese encephalitis (je) in whom jev-specific igm antibody was last detected 116 to 350 days after their acute illness. these six patients were compared with 94 other je patients matched for age, sex and serological response and in whom jev-specific igm was either short-lived (less than 90 days) or not tested. all patients were evaluated for the presence or absenc ...1976183556
actions and interactions of theophylline and imidazole on cardiac contractility, phosphorylase activation and cyclic amp.injection of 1.0 mg theophylline and 1.6 mg imidazole into guinea-pig hearts perfused by the langendorff technique increased cardiac contractile force by 20.0 +/- 1.0% and 23.3 +/- 1.2% over control and activated phosphorylase without producing significant changes in cyclic amp levels. when guinea-pig hearts were perfused with theophylline (10(-3) m) or imidazole (20 mm), injection of either imidazole or theophylline did not further increase cardiac contractility or phosphorylase a levels. in al ...1976183614
comparative studies on mitochondrial coupling factor b and fb. 1976183765
protection by propylthiouracil against carbon tetrachloride-induced liver damage.rats given a single intragastric dose of carbon tetrachloride (ccl4), 0.25, 0.50, or 1.0 ml per kg) showed a dose-dependent increase in sgot, serum ornithine carbamyltransferase, and liver necrosis (graded histologically as 0 to 4+) 24 hr after the treatment. daily intubation with propylthiouracil (ptu) for 10 days in doses of 5 to 50 mg per kg significantly reduced the elevation of sgot activity, completely suppressed the serum ornithine carbamyltransferase changes, and reduced the degree of ne ...1976184009
susceptibility of "enterobacteria" to aminoglycoside antibiotics: comparisons with tetracyclines, polymyxins, chloramphenicol, and spectinomycin.strains of enterobacteria, including common, aerobic, pathogenic gram-negative bacilli, and enterococci were tested for susceptibility to 11 aminoglycoside antibiotics by a twofold agar-dilution method with an inocula replicator. for comparison, similar tests were done with seven tetracycline analogues, two polymyxins, chloramphenicol, and spectinomycin. tobramycin compared favorably with the more active aminoglycosides, with some exceptions related to individual strains of species. the tetracyc ...1976184213
reduction in acetylcholine sensitivity of axotomized ciliary ganglion cells.1. isolated cell clusters from ciliary ganglia of 3- to 4-day-old chickens were used to examine the electrical characteristics and sensitivity to iontophoretically applied acetylcholine (ach) of normal cells and cells that had been axotomized on the day of hatching. 2. resting potentials, input resistances and capacitances were the same in axotomized cells as in normal cells. these averaged about 70 mv, 165 momega and 35 pf respectively. 3. sensitivity to iontophoretically applied ach was lower ...1976184272
persistence of antibody to feline panleucopaenia induced by a modified live virus vaccine. 1976184348
aspects of the influence of parasympathetic stimulation on aqueous humour dynamics in the rabbit [proceedings]. 1975184666
substrate cycles in metabolic regulation and in heat generation.1. the presence of substrate cycles in tissues has been demonstrated by direct isotope methods in recent years. this demonstration has provided the impetus for a reappraisal of the roles of substrate cycling in metabolic regulation and in heat production. these aspects of substrate cycling are discussed in this paper. the relationship between near-equilibrium reactions and substrate cycles is emphasized, since this provides a basis for the derivation of a function describing in precise quantitat ...1976184791
the induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface.rat ascites hepatoma ah109a cells (present as a free form in vivo) can aggregate and then develop well-defined tripartite junctional complexes, including intermediate junctions, desmosomes and focal tight junctions, on incubation with a glycoprotein separated from rat ascites hepatoma ah136b cells (forming cell islnds in vivo). the development of binding structures was strongly inhibited by actinomycin d. ah109a cells or rat ascites hepatoma ys cells (present as a free form in vivo) previously t ...1976184812
electron spin resonance study of the synaptosome opiate receptor: kinetics of stereospecific binding of spin labeled morphine.morphine, spin labeled on the 3- or 6-position has been used as the opiate ligand in a study of the time course of stereospecific opiate binding to intact synaptosomes isolated from non-cerebellar rat brain. the broadening of electron spin resonance lines induced by immobilization of the ligand on binding has been used to determine the concentration of bound opiate. the stereospecificity of the reaction was measured by comparing ligand binding in the presence of thousand-fold molar excesses of d ...1976184858
naltrexone-induced hypothermia in the rat.naltrexone, in relatively high doses, has been reported to cause a fall in body temperature in human ex-heroin addicts who had been abstinent for at least 6 weeks. the underlying mechanism of this hypothermic effect has been investigated in rats. the first consideration was that the temperature change was a reflection of delayed withdrawal but rats implanted with a morphine pellet 45 days earlier showed no significant change in temperature after a dose of naltrexone that caused marked withdrawal ...1976185061
the secretion of lysosomal enzymes.the studies reviewed in this chapter provide further evidence that the secretion of lysosomal enzymes and other hydrolases is a constitutive function of certain cells whereas in other cells is an inducible process probably contributing to the pathology of a variety of diseases. little is known of the mechanisms mediating the secretion of lysosomal enzymes. we have summarized evidence suggesting a role of microfilaments and microtubules in controlling enzyme release, but further studies of the bi ...1976185100
chirality of the hydrogen transfer to the coenzyme catalyzed by ribitol dehydrogenase from klebsiella pneumoniae and d-mannitol 1-phosphate dehydrogenase from escherichia coli.the stereochemistry of the hydrogen transfer to nad catalyzed by ribitol dehydrogenase (ribitol:nad 2-oxidoreductase, ec from klebsiella pneumoniae and d-mannitol-1-phosphate dehydrogenase (d-mannitol-1-phosphate:nad 2-oxidoreductase, ec from escherichia coli was investigated. [4-3h]nad was enzymatically reduced with nonlabelled ribitol in the presence of ribitol dehydrogenase and with nonlabelled d-mannitol 1-phosphate and d-mannitol 1-phosphate dehydrogenase, respectively. ...1976185137
inter- and intralaboratory variability in antibiotic susceptibility tests with pseudomonas aeruginosa and enterobacteriaceae.a two-phase collaboratice study was designed for definition of the extent to which results of disk diffusion tests can be reproduced. two currently recommended techniques, the kirby-bauer method and the agar overlay method, were used. of special concern were tests of pseudomonas aeruginosa with moderate susceptibility to carbenicillin; gentamicin, tobramycin, polymyxin b, and colistin were also tested with p. aeruginosa as well as with klebsiella pneumoniae, escherichia coli, and proteus species ...1976185302
normal rabbit alveolar macrophages. ii. their primary and secondary lysosomes as revealed by electron microscopy and this investigation, vacuoles containing tubular myelin proved to be digestive compartments with cytochemical reactivity for acid phosphatase and arylsulfatase. these cytochemical markers identify the secondary lysosomes, known to contain enzymes capable of hydrolyzing phospholipids like surfactant. therefore, it appears that alveolar macrophages possess the enzymatic machinery for the degradation of the tubular myelin found in their digestive vacuoles. although it thus appears evident that al ...1976185318
demyelinative myelopathy in mice induced by the da attenuated tissue culture adapted strain of da virus, an agent related to the theiler murine encephalomyelitis viruses (tmev), was used to induce a chronic myelopathy in mice. spastic paraparesis first appeared 5 months after weanings were inoculated intracerebrally with the virus. none died as a direct result of the infection, and none improved once paretic. the major pathological change in these mice was demyelination of thoracic segments of spinal cord. no clinical illness or demyelinative ...1976185333
enumeration of antigen reactive t cells against sheep erythrocytes by the virus plaque assay. 1976185443
effects of (+)-tubocurarine on neuromuscular facilitation and depression in rat diaphragm. 1976185539
novel structure at 5'-ends of nascent dna chains.because of their association with protein short nascent dna chains in escherichia coli can be separated from other cellular dna by chromatography on hydroxylapatite. protein-free dna chains of less than 500 nucleotides in length are resistant to degradation from the 5'-end by alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); ec] and spleen phosphodiesterase (oligonucleate 3'-nucleotidohydrolase; ec in contrast, dna chains containing more than ...1976185611
opiate receptor: autoradiographic localization in rat brain.opiate receptor sites in rat brain can be labeled in vivo by [3h]diprenorphine, a potent opiate antagoinst. using techniques to minimize diffusion in fresh, frozen, unfixed brain, we have localized [3h]diprenorphine by autoradiography to visualize the distribution of opiate receptors. silver grains indicative of the binding of labeled [3h]diprenorphine are discretely localized in numerous areas of the brain with very high densities in the locus coeruleus, the substantia gelatinosa of the spinal ...1976185626
chirality of the hydrogen transfer to nad catalyzed by myo-inositol dehydrogenase from klebsiella pneumoniae.chirality, hydrogen transfer, myo-inositol dehydrogenase. the chirality of the hydrogen transfer to nad catalyzed by myo-inositol dehydrogenase (myo-inositol: nad 2-oxydoreductase, ec from klebsiella pneumoniae (formerly classified taxonomically as aerobacter aerogenes or kleb siella aerogenes) was investigated. [4-3h] nad was enzymatically reduced to [4-3h] nadh with non-labeled myoinositol and myo-inositol dehydrogenase. the stereochemistry of the prochiral center at c4 of the nadh p ...1976185832
nucleotide sequence study of mouse 5.8s ribosomal rna.the primary structure of 5.8s mouse ribosomal rna has been studied and compared to the structures previously established for other animal species. the results obtained show that mouse 5.8s ribosomal rna yields pancreatic oligonucleotides with the same nucleotide sequence as the homologous oligonucleotides from rat cells. furthermore t1 oligonucleotides of 5.8s ribosomal rna from rat, mouse and human cells behave identically on fingerprinting fractionation and have the same composition as judged ...1976186131
exocrine pancreas under experimental conditions. ii. in vitro formation of paracrystalline structures and its functional consequences.the in vitro formation of paracrystalline structures after addition of high concentrations of amino acids to the incubation medium was investigated in pancreatic lobules and pancreatic homogenates. it could be shown that even in the homogenate, pcpa induced the formation of paracrystalline structures which exhibited the same ultrastructural arrangement as seen in the in vivo induced paracrystalline inclusions in the rer of the rat pancreas. in correlation with the morphological alterations, the ...1976186191
stimulation of lymphocytes by allogeneic lymphocytes and lymphoblasts in the presence of anti-hla antisera.anti-hla alloantisera inhibit mixed lymphocyte responses in which normal lymphocytes are used as stimulator cells. these same antisera are unable to inhibit lymphocyte proliferative responses stimulated by lymphoblastoid cells from cultured lymphoid cell lines. they also fail to inhibit either the generation of cytotoxic effector cells by lymphoblastoid cells or lymphocyte-mediated cytotoxicity against the lymphoblasts. although the number of hla antigens on the surface of lymphoblasts is report ...1976186270
adenosine promoted accumulation of adenosine 3',5'-monophosphate in rabbit vagus nerve.desheathed rabbit vagus nerve has been found to form cyclic amp when incubated with adenosine. this accumulation of cyclic amp is inhibited by theophylline but not by antiadrenergic agents, anticholinergic agents or local anaesthetics. depolarizing media are not able to promote cyclic amp accumulation in this preparation.1976186298
measurement and partial characterization of immunoreactive glucagon in gastrointestinal tissues of dogs.we have reported previously that increasing amounts of immunoreactive glucagon (irg), measured by four specific antisera, appeared in plasma of depancreatized insulin-deficient dogs. it was therefore concluded that pancreatectomy was not accompanied by glucagon deficiency in the dog, but instead excessive amounts of extrapancreatic irg could contribute to the diabetic syndrome. in order to locate the source of extrapancreatic glucagon, tissue extracts were assayed with anti-glucagon sera 30-k an ...1976186345
histochemistry of oxidative enzymes in experimental allergic encephalomyelitis.the histoenzymic pattern of oxidative enzymes (g3pd, idh, sd, g6pd,hbd, nadph: dehydrogenase) was investigated in experimental allergic encephalomyelitis (eae) produced in rats according to paterson [13]. the results obtained lead to following conclusions: (1) the neuroglia, including the white matter oligodendroglia of immunized rats, exhibits increased oxidoreductase activities; (2) the neuroallergic reaction induces some stimulation of the oxidoreductive metabolism of oligoden-droglia; (3) th ...1976186370
regulation of low density lipoprotein receptor activity in freshly isolated human lymphocytes.circulating human lymphocytes freshly isolated from venous blood of 15 normal subjects exhibited a low capacity to bind, take up, and degrade 125i-labeled low density lipoprotein (ldl). however, when these cells were incubated for 72 h in the absence of lipoproteins, they gradually acquired in increased number of high affinity cell surface receptors for ldl. the increase in the number of ldl receptors was associated with a 16-fold increase in the rate at which the cells were able to take up and ...1976186492
the effect of cyclic amp on catabolite repression of isocitrate lyase in nocardia salmonicolor (ncib9701). 1976186557
adrenocorticotropin. 49.1 synthesis and biological activity of [2-delta-aminovaleric acid, 5-arginine-a1adrenocorticotropin-(2-19).an adrenocorticotropin analogue, [2-delta-aminovaleric acid, 5-arginine]adrenocorticotropin-(2-19), has been synthesized by the solid-phase method and its biological activity has been determined. it was found that substitution of arginine for glutamic acid at position 5 of [2-delta-aminovaleric acid]adrenocorticotropin-(2-19) increased the steroidogenic potency in idolated rat adrenal cells and the lipolytic potency in isolated rat fat cells but decreased the lipolytic potency in isolated rabbit ...1976186605
enzymic activities of cadmium- and zinc-tolerant strains of klebsiella (aerobacter) aerogenes growing in glucose-limited chemostats.the activities of phosphatases and some enzymes of glucose metabolism were determined in k. aerogenes ncib 418 and in two strains derived from it, resistant to 50 mug cd2+ ml-1 and 16 mug zn2+ ml-1 respectively, during growth at d = 0.38 h-1 in medium containing beta-glycerophosphate as sole phosphorus source and supplemented with cd2+ and zn2+, as appropriate, for the resistant strains. the ph-activity profiles of the phosphatases differed from strain to strain but all showed maximum activity a ...1976186694
serum glycerol and hepatic glycerokinase activity in the carbohydrate-sensitive bhe strain of rat. 1976186795
stimulation by tsh of prostaglandin synthesis in pig thyroid.the liberation of arachindonate in the thyroid occurs at the expense of two distinct pools of precursors. (1) the phosphatidylinositol through a process ca2+-dependent and cyclic amp-independent; and (2) the triglycerides by a cyclic amp-dependent lipase, in which the involvement of cyclic amp-dependent protein kinase has not yet been determined. the "pi pool" or "paracyclic amp pool" is mobilized very rapidly by large doses of tsh but its physiological significance can be discussed. the "trigly ...1976187042
isolation and characterization of a virus associated with progressive pneumonia (maedi) of sheep.a virus with growth and morphologic characteristics of progressive pneumonia (maedi-visna) virus was isolated from the lungs of sheep with typical clinical and postmortem changes of chronic progressive pneumonia. the virus grew slowly in cultures of embryonic ovine lung cells, causing syncytial formation and degeneration. syncytia developed much slower and involved fewer cells than reported for other similar viruses isolated from sheep. as seen with the electron microscope, the virus reproduced ...1976187087
[the quantitative cytoarchitecture of morris hepatoma 66 (author's transl)].introduction and methods: to obtain a statistic comparable picturg oncogenesis, tissue of a middle-fast growing morris-hepatoma 66 was transplanted into the femoral musculature. 35 days later the animals were killed by decapitation and hepatoma tissue was taken. the morphometric analysis based on the technique described by weibel (1969). results and discussion: in the morris-hepatoma 66 hepatocyte and nucleus show a much smaller single volume in comparison to normal liver cell. smaller is also t ...1976187163
transfer of parental immunity to infectious laryngotracheitis in chicks.the transfer of parental immunity to infectious laryngotracheitis was appraised by measuring serum antibody levels in 150 chicks from the day of hatch up to five weeks. the breeder flock which had received primary vaccination at eight weeks and a booster at 20 weeks transferred high antibody levels which fell markedly within two weeks and remained constant thereafter. chicks whose parents were vaccinated at 20 weeks only, had low antibody levels throughout. these low levels, in either group of c ...1976187298
[comparison between the official indirect method and the direct method of control of vaccines against sheep enterotoxemia].the official methods of control of vaccines against sheep enterotoxemia are indirect in two ways : on the one hand they are carried out on animals for which the vaccine is not intended, and on the other hand they test only an immunological serum reaction. another method consisting of direct testing by intravenous injection of toxin has been carried out on mice previously vaccinated with different doses. it has been shown that it is possible to obtain a certain level of protection giving a good d ...1976187512
an experimental animal model for the study of human scirrhous carcinoma.scirrhous-like carcinomas were induced by the inoculation of cloned mouse mammary carcinoma cells into the cleared fat pads of balb/c mice. the inoculated cells induced palpable tumors in 100% of the animals within 60 days. the epithelial cells in vivo grew in rows and clusters and were attached by short developing desmosomes. microvilli and organelles were scarce; intracisternal type a particles were observed in all the epithelial cells. carcinoma cells invaded the dermis and muscle. the stroma ...1976187797
enhancement of virus-induced cell fusion by phytohemagglutinin. 1976187897
[the concept of risk in poliomyelitis (author's transl)].in poliomyelitis the risk is of neurological accidents resulting either from the circulation of wild viruses (spontaneous risk) or from the introduction of virus-vaccine (vaccine risk). the spontaneous risk varies both according to the virus type and according to various factors which determine host resistance. well known among these are: the intrinsic characteristics of the population at a given period (genetic factors and previous experiences of this population with poliomyelitis viruses); env ...1976188083
[reducing the risk of tuberculosis in children vaccinated with bcg].in 1973, of 1774192 children aged 18 months to 6 years, 82,2% had to be revaccinated with bcg, 10.7% presented tuberculin reactions larger than 10 mm and 7.1% were absentees. in 1974 a number of 485 children belonging to the respective age group contracted tuberculosis. analysis of these cases showed that the risk of tuberculosis was of 10.9 per 100,000 revaccinated subjects and of 74.6 per 10,000 absentees. bcg vaccination not only lowered the risk of the disease but also reduced the risk of a ...1976188106
[effects of the nervous system on the kidney. part i. review of the literature].the authors review the literature on the effects of the nervous system on the kidney in 3 stages: 1) historical stage when the role of the renal nerve supply was overetimated by claude bernard. 2) intermediate stage between the two wars with a clinical description of uremic encephalits or acute delirium. from the experimental point of view was demonstrated the inessential character of the nerve supply for the survival of the kidney. 3) modern stage: from the clinical point of view one may distin ...1976188168
[spondylodiskitis caused by klebsiella pneumoniae, an unusual complication of diverticular sigmoditis]. 1976188196
phosphorylation in membranes of intact human erythrocytes.studies of phosphorylation in membranes of intact human erythrocytes were performed by incubating erythrocytes in inorganic [32p]phosphate. analysis of membrane proteins by polyacrylamide gel electrophoresis showed a pattern of phosphorylation similar to that observed when ghost membranes were incubated with [gamma-32p]atp. membrane lipid phosphorylation was also similar in intact cells and ghosts. the most heavily phosphorylated lipid, polyphosphoinositide, was closely associated with glycophor ...1977188816
inoculation experiment of marek's disease vaccine contaminated with a reticuloendotheliosis virus.two-day-old chicks were inoculated with one or ten doses of marek's disease (md) vaccine originated from the herpesvirus of turkeys (hvt) and contaminated with a reticuloendotheliosis virus (rev). as a result, they presented such symptoms as abnormality in the vane of remiges, undergrowth, anemia, and leg paralysis. these symptoms were the same as those induced by the same vaccine among chicks in the field. control chicks which had been placed in the same house as those inoculated with the vacci ...1976189219
acth and brain rna: changes in content and labelling of rna in rat brain stem.the influence of synthetic acth1-24 and acth1-10 on rat brain rna was studied. acth1-24 (5 iu/100 g s.c.) treatment resulted in a small decrease in labelling of brain stem rna (-12%), measured 30 min after the injection of [5-3h] uridine (100 muci; s.c.), without affecting the distribution of radioactivity of the acid-soluble precursor pool. furthermore, a small and transitional reduction of total brain stem rna was found. similar treatment of adrenalectomized rats (36 h after surgery) resulted ...1976189247
cholecalciferol metabolites binding in porcine parathyroid glands.we studied the cytoplasmic and nuclear binding of 25-hydroxychole-calciferol and 1alpha,25-dihydroxycholecalciferol inside porcine parathyroid glands. both sterols bind to cytoplasmic components, but a specific nuclear uptake was demonstrated only for 1alpha,25-dihydroxycholecalciferol. these findings support the hypothesis that mammalian parathyroid glands are a target organ for some cholecalciferol metabolites.1976189464
[microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses].the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ...1976190825
rat adrenocortical dynamics.1. the dynamics of the adrenocortical response to adrenocorticotrophic hormone (acth) was studied in anaesthetized, acutely hypophysectomized male rats. 2. acth test-signals were applied either in a jugular vein ('intact infused preparation') or in the aorta through the coeliac artery with the aorta ligated below this artery ('isolated in situ perfused preparation'). the adrenocortical responses were measured directly in samples serially taken from the left adrenal vein. 3. tested acth signals w ...1977191596
postmenopausal osteoporosis: the effect of parathormone and large dose vitamin d3 on the serum calcium level in sex hormone deficient rats.rats deprived of adrenal and gonadel sex hormones are more sensitive than normal rats to the hypercalcaemic (osteolytic) effect of parathormone and toxic doses of vitamin d3. it is suggested that sex hormone deficiency and the consecutive decrease of calcitonin sensitivity in postmenopausal osteoporosis makes the patients unprotected against factors inducing increased bone resorption, and this leads over the years of osteoporosis.1975192029
factors affecting the binding of [3h]adenosine 3':5'-cyclic monophosphate to protein kinase from bovine adrenal cortex.inorganic salts, several proteins and traces of protein precipitants were tested to find out by what mechanisms they modulate the binding of cyclic [3h]amp to protein kinase (atp-protein phosphotransferase; ec the separation of free and bound cyclic amp by (nh4)2so4 precipitation was unaffected by the above agents and was more reliable than the millipore filtration technique. several binding sites for cyclic amp were revealed in adrenal-cortex extract. when this extract was used as bi ...1977192203
effects of prostaglandin on substrate uptake and cell division in human diploid (prostaglandin) e1 inhibits the uptake of iridine, thymidine, 2-deoxy-d-glucose and l-isoleucine into human diploid wi38 fibroblasts. the inhibition occurs within seconds of the addition of the prostaglandin to the culture. pge2, pgf1alpha and pgf2alpha behave similarly. arachidonic acid and 8,11,14-eicosatrienoic acid also decrease uptake in the presence or absence of indomethacin. other unsaturated fatty acids such as oleic acid, linoleic acid and linolenic acid are essentially inactive. ri ...1977192204
putrescine transport is greatly increased in human fibroblasts initiated to proliferate.putrescine (diaminobutane) was previously found to stimulate proliferation of human fibroblasts in tissue culture, and a growth factor produced by these cells was identified as putrescine. in the present paper putrescine transport is studied. the rate of putrescine transport was dependent on temperature, and most of the labeled putrescine was retained by the cells after washing with excess unlabeled putrescine. the concentration of radioactivity after a [14 c]putrescine pulse was 85 times higher ...1976192729
expression of klebsiella nif and his genes in salmonella typhimurium.derivatives of salmonella typhimurium carrying f prime or p prime plasmids with klebsiella nif and his genes had specific nitrogenase activities similar to klebsiella in selective conditions, even to showing "hyperinduction" under argon. no evidence was obtained for catabolite repression of normal nif expression but dibutyl cyclic amp often augmented "hyperinduction". in non-selective conditions the klebsiella his nif determinants were rapidly lost from the plasmids; the low levels of nif expres ...1977192833
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