Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| copper is a host effector mobilized to urine during urinary tract infection to impair bacterial colonization. | urinary tract infection (uti) is a major global infectious disease affecting millions of people annually. human urinary copper (cu) content is elevated during uti caused by uropathogenic escherichia coli (upec). upec upregulates the expression of cu efflux genes during clinical uti in patients as an adaptive response to host-derived cu. whether cu is mobilized to urine as a host response to uti and its role in protection against uti remain unresolved. to address these questions, we tested the hy ... | 2017 | 28031261 |
| emergence of ceftazidime-avibactam resistance due to plasmid-borne blakpc-3 mutations during treatment of carbapenem-resistant klebsiella pneumoniae infections. | ceftazidime-avibactam is a novel β-lactam/β-lactamase inhibitor with activity against carbapenem-resistant enterobacteriaceae (cre) that produce klebsiella pneumoniae carbapenemase (kpc). we report the first cases of ceftazidime-avibactam resistance to develop during treatment of cre infections and identify resistance mechanisms. ceftazidime-avibactam-resistant k. pneumoniae emerged in three patients after ceftazidime-avibactam treatment for 10 to 19 days. whole-genome sequencing (wgs) of longit ... | 2017 | 28031201 |
| distribution of ctx-m group i and group iii β-lactamases produced by escherichia coli and klebsiella pneumoniae in lahore, pakistan. | extended-spectrum-lactamases (esbls) of the ctx-m type is worrisome issue in many countries of the world from past decade. but little is known about ctx-m beta-lactamase producing bacteria in pakistan. therefore, this study was carried out to investigate the distribution of ctx-m beta-lactamase producing e. coli and klebsiella pneumoniae using phenotypic and molecular techniques. a total of 638 e. coli and 338 klebsiella pneumoniae were isolated from patients attending two hospitals and one diag ... | 2017 | 27956216 |
| detection of acra, acrb, aac(6')-ib-cr, and qepa genes among clinical isolates of escherichia coli and klebsiella pneumoniae. | the distribution of drug resistance among clinical isolates of escherichia coli and klebsiella pneumoniae has limited the therapeutic options. the aim of this study was to report the prevalence of quinolone resistance genes among e. coli and k. pneumoniae clinical strains isolated from three educational hospitals of tehran, iran. | 2017 | 27936860 |
| antioxidant, anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica. | stachys guyoniana noë ex. batt. and mentha aquatica l. are two algerian lamiaceae used in folk medicine. | 2017 | 27927090 |
| multidrug resistant enterobacteriaceae in new zealand: a current perspective. | in this article we review mechanisms and potential transmission pathways of multidrug resistance in enterobacteriaceae, with an emphasis on extended-spectrum β-lactamase (esbl)-production. this provides background to better understand challenges presented by this important group of antimicrobial resistant bacteria, and inform measures aimed at prevention and control of antimicrobial resistance in general. humans and animals interact at various levels; household pets cohabit with humans, and othe ... | 2017 | 27927087 |
| extended spectrum cephalosporin resistance among clinical isolates of enterobacteriaceae in west norway during 2006-2013; a prospective surveillance study. | routine surveillance of resistance to broad-spectrum cephalosporins in enterobacteriaceae and phenotypic identification of underlying mechanisms using a simple strategy was commenced in 2006 at our laboratory, serving west norway. this report focuses on the results until 2013. the classical plasmid-mediated extended spectrum beta-lactamase (esbla ) among clinically relevant escherichia coli isolates showed an increase from 0.6% to 4.3% during the surveillance period, while prevalence for other m ... | 2017 | 27921337 |
| detection of escherichia coli st131 clonal complex (st705) and klebsiella pneumoniae st15 among faecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae. | the objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-e) and carbapenemase-producing enterobacteriaceae (cpe) in non-selected hospitalized and non-hospitalized patients from the same geographic area of madrid. | 2017 | 27902381 |
| investigation of carbapenem resistance and the first identification of klebsiella pneumoniae carbapenemase (kpc) enzyme among escherichia coli isolates in turkey: a prospective study. | the aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in escherichia coli isolates from clinical samples in turkey. | 2017 | 27890667 |
| peripartum maternal transmission of extended-spectrum β-lactamase organism to newborn infants. | the aim of this study was to determine whether the route of extended-spectrum β-lactamase (esbl) transmission to hospitalized newborns was from their mothers during delivery. neonatal intensive care unit (nicu) hospitalized newborns were sampled for esbl presence by stool cultures on the first and fourth days of life. mothers of esbl-positive newborns were sampled for possible correlation detection. bacteria isolates were molecularly identified and susceptibility tests for antibiotic agents were ... | 2017 | 27889252 |
| clbm is a versatile, cation-promiscuous mate transporter found in the colibactin biosynthetic gene cluster. | multidrug transporters play key roles in cellular drug resistance to toxic molecules, yet these transporters are also involved in natural product transport as part of biosynthetic clusters in bacteria and fungi. the genotoxic molecule colibactin is produced by strains of virulent and pathobiont escherichia coli and klebsiella pneumoniae. in the biosynthetic cluster is a multidrug and toxic compound extrusion protein (mate) proposed to transport the prodrug molecule precolibactin across the cytop ... | 2017 | 27939886 |
| piperacillin/tazobactam as an alternative antibiotic therapy to carbapenems in the treatment of urinary tract infections due to extended-spectrum β-lactamase-producing enterobacteriaceae: an in silico pharmacokinetic study. | piperacillin/tazobactam (tzp) as an alternative treatment to carbapenems for infections involving extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-pe) remains debated. in this study, the probabilities of pharmacodynamic (pd) target attainment with different tzp regimens in esbl-producing escherichia coli (esbl-ec) and klebsiella pneumoniae (esbl-kp) were evaluated in the context of pyelonephritis. minimum inhibitory concentrations (mics) of 144 esbl-ec and 111 esbl-kp from ... | 2017 | 27876276 |
| characterization of ctx-m enzymes, quinolone resistance determinants, and antimicrobial residues from hospital sewage, wastewater treatment plant, and river water. | multidrug-resistant (mdr) bacteria are widespread in hospitals and have been increasingly isolated from aquatic environments. the aim of the present study was to characterize extended-spectrum β-lactamase (esbl) and quinolone-resistant enterobacteriaceae from a hospital effluent, sanitary effluent, inflow sewage, aeration tank, and outflow sewage within a wastewater treatment plant (wwtp), as well as river water upstream and downstream (urw and drw, respectively), of the point where the wwtp tre ... | 2017 | 27816836 |
| occurrence of carbapenemase-producing klebsiella pneumoniae and escherichia coli in the european survey of carbapenemase-producing enterobacteriaceae (euscape): a prospective, multinational study. | gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in europe make it difficult to contain carbapenemase-producing enterobacteriaceae. we report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing klebsiella pneumoniae and escherichia coli in european hospitals. | 2017 | 27866944 |
| outbreak of kpc-3-producing st15 and st348 klebsiella pneumoniae in a portuguese hospital. | to date, only a few sporadic cases of infections due to klebsiella pneumoniae carbapenemase (kpc) producers have been reported in portugal. here, we report for the first time an outbreak of k. pneumoniae kpc-3 producers in a tertiary-care hospital during 2013. twenty-seven ertapenem-resistant k. pneumoniae were identified in patients at a tertiary-care hospital during 2013 isolated predominantly from urine (48·1%) and blood (25·9%) cultures. all isolates were highly resistant to β-lactam antibio ... | 2017 | 27788691 |
| engineering plasmid-free klebsiella pneumoniae for production of 3-hydroxypropionic acid. | although plasmid-dependent microbial breeding is predominant in manufacturing bio-based chemicals, it shows pitfall of genetic instability and thus hinders industrial production. alternatively, chromosome engineering free from plasmid enables genetic stability and thus represents the new trend of microbial breeding. 3-hydroxypropionic acid (3-hp) is an economically important platform compound as the versatile precursor of a suite of c3 compound, such as 1, 3-pdo. klebsiella pneumoniae is regarde ... | 2017 | 27787604 |
| infectious agents after liver transplant: etiology, timeline and patients' cell-mediated immunity responses. | infections continue to be one of the leading causes of morbidity and mortality in liver transplant recipients. we retrospectively reviewed the symptomatic infectious episodes that occurred during the first year post-transplant to determine time of onset, causative pathogens and cell-mediated immunity response patterns. ninety-eight of the 202 (48.5%) recipients enrolled developed at least one infectious episode. the total number of infectious episodes was 135: 77 (57.1%) bacterial, 45 (33.3%) vi ... | 2017 | 27783145 |
| extended-spectrum β-lactamase- and pampc-producing enterobacteriaceae among the general population in a livestock-dense area. | in the netherlands there is an ongoing debate regarding environmental health risks of livestock farming for neighbouring residents. this explorative study aims to determine the prevalence of carriage of extended-spectrum β-lactamase and/or plasmid-mediated ampc-producing enterobacteriaceae (esbl/pampc-e) in the general population living in a livestock-dense area, and to study associations between determinants, including exposure through contact with animals and the environment, and human carriag ... | 2017 | 27773759 |
| molecular characteristics of extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae causing intra-abdominal infections from 9 tertiary hospitals in china. | recently, the emergence of multidrug-resistant organisms such as extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae has raised considerable concern regarding the appropriate treatment of intra-abdominal infections (iais). in this study, we investigated the molecular characteristics of esbl among clinical isolates of escherichia coli and klebsiella pneumoniae causing iais and their pattern of antimicrobial resistance, which can provide useful information about the epidemiology and ... | 2017 | 27773544 |
| high prevalence of esbl-producing escherichia coli and klebsiella pneumoniae in community-onset bloodstream infections in china. | community-onset bloodstream infections (cobsis) caused by esbl-producing escherichia coli (esbl-ec) and esbl-producing klebsiella pneumoniae (esbl-kp) are increasing globally. this study aimed to investigate the epidemiology and risk factors of esbl-ec and esbl-kp in cobsis in china. | 2017 | 27624571 |
| design, synthesis, and evaluation of pyrazolo-pyrazole derivatives on methylisocitratelyase of pseudomonas aeruginosa: in silico and in vitro study. | pseudomonas aeruginosa is an opportunistic micro-organism causing diseases both in animals and humans. in case of human pathology, the role of p. aeruginosa is one of the major concerns in intensive care septicemia. presently, the drug resistance strains of p. aeruginosa are arising mainly by developing multiple mechanisms due to its natural and acquired resistance to many of the antimicrobial agents commonly used in clinical practice. as a result, there is a direct need to invent new drugs so t ... | 2017 | 27686121 |
| preparation of thermosensitive gel for controlled release of levofloxacin and their application in the treatment of multidrug-resistant bacteria. | levofloxacin is a synthetic broad-spectrum antibacterial agent for oral or intravenous administration. chemically, levofloxacin is the levorotatory isomer (l-isomer) of racemate ofloxacin, a fluoroquinolone antibacterial agent. quinolone derivatives rapidly and specifically inhibit the synthesis of bacterial dna. levofloxacin has in vitro activity against a broad range of aerobic and anaerobic gram-positive and gram-negative bacteria. however, formulation of combined poloxamers thermoregulated ( ... | 2017 | 27689094 |
| microangiopathic antiphospholipid antibody-associated syndrome in a pregnant lady. | we describe a gravid 37-year-old chinese lady with known triple positive primary antiphospholipid syndrome with previous recurrent deep vein thrombosis and early spontaneous miscarriages. she was managed with low-molecular weight heparin, aspirin, hydroxychloroquine, prednisolone and monthly intravenous immunoglobulin. she presented with recurrent per-vaginal bleeding at 22 weeks of gestation and was found to have abruptio placentae. anti-coagulation was held off. she subsequently delivered a st ... | 2017 | 27694537 |
| efficacy of ceftolozane/tazobactam against urinary tract and intra-abdominal infections caused by esbl-producing escherichia coli and klebsiella pneumoniae: a pooled analysis of phase 3 clinical trials. | the increase in infections caused by drug-resistant esbl-producing enterobacteriaceae (esbl-ent) is a global concern. the characteristics and outcomes of patients infected with esbl-ent were examined in a pooled analysis of phase 3 clinical trials of ceftolozane/tazobactam in patients with complicated urinary tract infections (aspect-cuti) and complicated intra-abdominal infections (aspect-ciai). | 2017 | 27707990 |
| first description of kpc-2-producing escherichia coli and st15 oxa-48-positive klebsiella pneumoniae in tunisia. | the aim of this study was to investigate the molecular features among klebsiella pneumoniae and escherichia coli strains showing a resistant/intermediate-resistant phenotype to ertapenem (r/ir-ert), implicated in colonization/infection in patients of the hematology and graft units of the national bone marrow transplant center of tunisia (3-year period, 2011-2014). the major carbapenemase, extended-spectrum beta-lactamase, and plasmidic ampc beta-lactamase genes were analyzed and characterized by ... | 2017 | 27754776 |
| an integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin. | this study attempts to evaluate the antimicrobial activity and the ecotoxicity of quantum dots (qds) alone and coated with indolicidin. to meet this objective, we tested the level of antimicrobial activity on gram-positive and gram-negative bacteria, and we designed an ecotoxicological battery of test systems and indicators able to detect different effects using a variety of end points. the antibacterial activity was analyzed against staphylococcus aureus (atcc 6538), pseudomonas aeruginosa (atc ... | 2017 | 27616887 |
| occurrence and characterization of blandm-5-positive klebsiella pneumoniae isolates from dairy cows in jiangsu, china. | to investigate the epidemiological characteristics and the surrounding genetic structure of the blandm-5 gene in klebsiella pneumoniae derived from dairy cows in jiangsu province, china. | 2017 | 27621177 |
| increasing incidence of carbapenemase-producing escherichia coli and klebsiella pneumoniae in belgian hospitals. | carbapenemase-producing enterobacteriaceae are increasingly reported worldwide. the aim of the study was to determine the incidence and molecular epidemiology of carbapenemase-producing (cp) escherichia coli and klebsiella pneumoniae (cp-e/k) in belgium. eleven hospital-based laboratories collected carbapenem non-susceptible (cns) isolates of e. coli and k. pneumoniae detected in clinical specimens from january 2013 to december 2014. all cns strains were tested for carbapenemase production and t ... | 2017 | 27639859 |
| low biological cost of carbapenemase-encoding plasmids following transfer from klebsiella pneumoniae to escherichia coli. | the objective of this study was to determine the biological cost, stability and sequence of two carbapenemase-encoding plasmids containing blakpc-2 (pg12-kpc-2) and blavim-1 (pg06-vim-1) isolated from klebsiella pneumoniae when newly acquired by uropathogenic escherichia coli clinical isolates of different genetic backgrounds. | 2017 | 27591293 |
| molecular characterization of cotrimoxazole resistance genes and their associated integrons in clinical isolates of gram-negative bacteria from tanzania. | cotrimoxazole is widely used, particularly as a prophylactic drug in hiv patients. we assessed resistance mechanisms among cotrimoxazole resistant-gram negative bacterial isolates (n = 123) obtained from blood (n = 69) and urine (n = 54) from tanzanian patients. sul genes were detected in 98% (121/123) of the isolates. coexistence of sul1 and sul2 was common (49/123). the dfr genes were found in 63% (77/123) of all isolates. sul1, dfra15, and dfra5 genes predominated among klebsiella pneumoniae, ... | 2017 | 27533639 |
| beyond susceptible and resistant, part iii: treatment of infections due to gram-negative organisms producing carbapenemases. | carbapenemases are enzymes that are capable of inactivating all or almost all beta-lactam antimicrobial agents. these enzymes are frequently coexpressed with other resistance mechanisms to non-beta-lactams, leading to extremely drug-resistant pathogens. once a curiosity, these enzymes have spread into organisms that are among the most common causes of infection, such as klebsiella pneumoniae and escherichia coli. identification of these organisms has proved challenging for clinical microbiology ... | 2017 | 27199618 |
| endemic indian clones of klebsiella pneumoniae-harbouring new delhi metallo-beta-lactamase-1 on a hybrid plasmid replicon type: a case of changing new delhi metallo-beta-lactamase plasmid landscapes in india? | blandm genes are mbl genes that confer resistance to carbapenems. globally, they are associated with diverse clones and plasmids. in this study, we characterised three isolates of klebsiella pneumoniae-harbouring blandm1 from patients undergoing chronic haemodialysis and renal transplantation. | 2017 | 27514948 |
| carbapenemase-producing enterobacteriaceae in the uk: a national study (euscape-uk) on prevalence, incidence, laboratory detection methods and infection control measures. | to estimate uk prevalence and incidence of clinically significant carbapenemase-producing enterobacteriaceae (cpe), and to determine epidemiological characteristics, laboratory methods and infection prevention and control (ipc) measures in acute care facilities. | 2017 | 27687074 |
| ertapenem in outpatient parenteral antimicrobial therapy for complicated urinary tract infections. | ertapenem is a broad-spectrum antibiotic that is increasingly being utilized. its dosing convenience renders it suitable for outpatient therapy, and its pharmacokinetic characteristics favour its use against complicated urinary tract infections (cutis). despite this, sufficient clinical data are lacking for its use against cutis in the outpatient setting. we assessed the microbiological and clinical cure rates associated with ertapenem treatment for cutis in two outpatient parenteral antimicrobi ... | 2017 | 27239695 |
| high prevalence of plasmid-mediated quinolone resistance determinants in escherichia coli and klebsiella pneumoniae isolates from pediatric patients in china. | in this study, the prevalence of the plasmid-mediated quinolone resistance (pmqr) genes among pediatric clinical isolates of escherichia coli and klebsiella pneumoniae was investigated. a total of 243 nonduplicate clinical isolates of e. coli and k. pneumoniae collected between january 2010 and december 2012 from beijing, china, were studied. in total, 55 isolates (22.63%) were positive for pmqr genes, the most frequently detected gene was qnrs (13.2%), followed by aac(6')-ib-cr (6.2%) and qnrb ... | 2017 | 27167505 |
| characterization of extended-spectrum β-lactamase-carrying plasmids in clinical isolates of klebsiella pneumoniae from taiwan. | we analyzed the replicon types, sizes, and restriction fragment length polymorphism (rflp) typing of plasmids carrying extended-spectrum β-lactamase (esbl) genes in klebsiella pneumoniae isolates from taiwan. fifty-one escherichia coli transconjugant strains with plasmids from esbl-producing k. pneumoniae from the taiwan surveillance of antimicrobial resistance iii program in 2002 were included. all the 51 plasmids carried a blactx-m gene, the majority of which were blactx-m-3 (28/51 [54.9%]). p ... | 2017 | 27148814 |
| in vitro antibacterial activity of aqueous and ethanol extracts of aristolochia indica and toddalia asiatica against multidrug-resistant bacteria. | bacteria have developed multidrug resistance against available antimicrobial agents. infectious diseases caused by these multidrug-resistant bacteria are major causes of morbidity and mortality in human beings. synthetic drugs are expensive and inadequate for the treatment of diseases, causing side effects and ineffective against multidrug-resistant bacteria. the medicinal plants are promising to have effective antimicrobial property due to presence of phytochemical compounds like alkaloids, fla ... | 2017 | 26997710 |
| bacteriology and antimicrobial susceptibility of esbls producers from pus in patients with abdominal trauma associated intra-abdominal infections. | intra-abdominal infections (iais) caused by esbls producing bacteria have become a serious clinical concern worldwide as the prevalence of bacterial resistance to antibiotics continues to increase. the objective of this study was to analyze the bacteriology and antimicrobial susceptibility of esbls producers using pus samples from iais patients caused by abdominal trauma. | 2017 | 26902656 |
| bacteriological profile and antibiotic susceptibility patterns of clinical isolates in a tertiary care cancer center. | this increased risk of bacterial infections in the cancer patient is further compounded by the rising trends of antibiotic resistance in commonly implicated organisms. in the indian setting this is particularly true in case of gram negative bacilli such as escherichia coli, klebsiella pneumoniae and acinetobacter spp. increasing resistance among gram positive organisms is also a matter of concern. the aim of this study was to document the common organisms isolated from bacterial infections in ca ... | 2017 | 27051152 |
| intestinal colonization by extended-spectrum beta-lactamase-producing enterobacteriaceae in infants. | members of the enterobacteriaceae family are common agents of nosocomial infections. intestinal colonization by these microorganisms represents a major step in the development of systemic infection. extended-spectrum b-lactamase-producing bacteria are usually associated with outbreaks, but endemic infections are common in intensive care units. | 2017 | 26696335 |
| profiles of acute bacterial meningitis isolates in children in national hospital, abuja. | acute bacterial meningitis (abm) is an important cause of morbidity and mortality throughout the world. it is an acute medical emergency that requires urgent rational antibiotic therapy, especially in neonates and young infants. determining the pattern and susceptibility of isolates of abm among children for prompt treatment of this important cause of mortality and morbidity is very important. this study determined the types and the antimicrobial susceptibility pattern of abm isolates among chil ... | 2017 | 26759518 |
| bacteriological profile of neonatal septicemia in a tertiary care hospital from western india. | neonatal septicemia is an important cause of morbidity and mortality. the present study was undertaken to determine the bacteriological profile and antimicrobial susceptibility pattern of prevalent pathogens isolated from the blood of septicemic neonates from neonatal intensive care unit (nicu). a total of 180 blood samples of septicemic neonates were studied bacteriologically. antimicrobial susceptibility testing was done by the kirby bauer disc diffusion method in accordance to clinical labora ... | 2017 | 26069427 |
| antimicrobial resistance of bacterial isolates from respiratory secretions of ventilated patients in a multi-specialty hospital. | ventilator-associated pneumonia (vap) is a common nosocomial infection occurring in intensive care unit (icu) settings. vap occurs due to interplay of three factors - impaired host defense, access of large numbers of pathogenic bacteria to the lower respiratory tract and the virulence of the organism. knowledge of colonizing microbial flora and their antibiogram in ventilated patients is of great importance in timely institution of empirical therapy, so that mortality and morbidity due to vap ca ... | 2017 | 26229758 |
| [incidence of urinary tract infections after cardiac surgery: comparative study accordind to catheterization device]. | urinary tract infections (uti) associated with urethral catheterization are the second cause of nosocomial infections in intensive care units. we confirm a uti when we get a result of 100,000 cfu/ml with at least one microorganism in a urine culture. | 2017 | 25804335 |
| resistance profile for pathogens causing urinary tract infection in a pediatric population, and antibiotic treatment response at a university hospital, 2010-2011. | urinary tract infection (uti) is one of the most common bacterial infections in childhood and causes acute and chronic morbidity and long-term hypertension and chronic kidney disease. | 2017 | 24970958 |
| emphysematous cystitis: an atypical multi-organism presentation. | an 84-year-old diabetic women receiving in-hospital stroke management began suffering from vague lower abdominal pain, increased urge incontinence and gross hematuria. physical examination revealed suprapubic tenderness and a tympanic bladder. a computed tomography confirmed a distended bladder with gas foci delineating the bladder wall. microbiology revealed an atypical multi-organism presentation of klebsiella pneumoniae and enterococcus species. emphysematous cystitis is characterized by air ... | 2017 | 24678369 |
| apoptosis, toll-like, rig-i-like and nod-like receptors are pathways jointly induced by diverse respiratory bacterial and viral pathogens. | lower respiratory tract infections are among the top five leading causes of human death. fighting these infections is therefore a world health priority. searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. with this aim, alveolar macrophages were independently infected with three unrelated bacterial (streptococcus pneumoniae, klebsiella pneumoniae, an ... | 2017 | 28298903 |
| presence, distribution and molecular epidemiology of multi-drug-resistant gram-negative bacilli from medical personnel of intensive care units in tianjin, china, 2007-2015. | multi-drug-resistant gram-negative bacteria (mdrgnb) have become an important cause of nosocomial infection in intensive care units (icus). | 2017 | 28268024 |
| targeting biofilms of multidrug-resistant bacteria with silver oxynitrate. | a topical antimicrobial, silver oxynitrate (ag7no11), has recently become available that exploits the antimicrobial activity of ionic silver but has enhanced activity because highly oxidised silver atoms are stabilised with oxygen in a unique chemical formulation. the objective of this study was to use a multifaceted approach to characterise the spectrum of antimicrobial and antibiofilm activity of a wound dressing coated with ag7no11 at a concentration of 0.4 mg ag/cm(2). physiochemical propert ... | 2017 | 28390963 |
| treating complicated carbapenem-resistant enterobacteriaceae infections with ceftazidime/avibactam: a retrospective study with molecular strain characterisation. | ceftazidime/avibactam (caz/avi) is the first antimicrobial agent with activity against carbapenem-resistant enterobacteriaceae (cre) approved by the us food and drug administration (fda). notably, human clinical outcome data for this indication are limited. therefore, a retrospective study was performed to evaluate the clinical outcomes and bacterial genomic characteristics of patients hospitalised at a tertiary medical centre with cre infections treated for the first time with caz/avi. from a t ... | 2017 | 28389354 |
| risk factors for kpc-producing enterobacteriaceae acquisition and infection in a healthcare setting with possible local transmission: a case-control study. | reports of klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae (kpc-kp) in australia were previously uncommon, with cases imported sporadically by travellers from higher prevalence countries. | 2017 | 28389093 |
| tigecycline resistance among carbapenem-resistant klebsiella pneumoniae: clinical characteristics and expression levels of efflux pump genes. | tigecycline is a treatment option for infections caused by carbapenem-resistant klebsiella pneumoniae (crkp). emerging tigecycline resistance in crkp represents a growing threat. knowledge of the clinical, microbiological, and molecular characteristics of tigecycline- and carbapenem-resistant klebsiella pneumoniae (tcrkp) is limited. | 2017 | 28388651 |
| molecular basis of substrate recognition and product release by the klebsiella pneumoniae carbapenemase (kpc-2). | carbapenem-resistant enterobacteriaceae (cre) are resistant to most β-lactam antibiotics due to production of the kpc-2 class a β-lactamase. here we present the first product complex crystal structures of kpc-2 with β-lactam antibiotics, containing hydrolyzed cefotaxime and faropenem. they provide experimental insights into substrate recognition by kpc-2 and its unique cephalosporinase/carbapenemase activity. these structures also represent the first product complexes for a wild type serine β-la ... | 2017 | 28388065 |
| virulence and genomic feature of a virulent klebsiella pneumoniae sequence type 14 strain of serotype k2 harboring blandm-5 in china. | the objective of this study was to reveal the molecular mechanism involved in carbapenem resistance and virulence of a k2 klebsiella pneumoniae clinical isolate 24835. the virulence of the strain was determined by in vitro and in vivo methods. the de novo whole-genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the carbapenem resistance and virulence of k. pneumoniae 24835. strain 24835 was highly resistant to carbapenems and belo ... | 2017 | 28386246 |
| the efficacy and safety of tigecycline for the treatment of bloodstream infections: a systematic review and meta-analysis. | patients with bloodstream infections (bsi) are associated with high mortality rates. due to tigecycline has shown excellent in vitro activity against most pathogens, tigecycline is selected as one of the candidate drugs for the treatment of multidrug-resistant organisms infections. the purpose of this study was to evaluate the effectiveness and safety of the use of tigecycline for the treatment of patients with bsi. the pubmed and embase databases were systematically searched, to identify publis ... | 2017 | 28381268 |
| correction: occurrence of diverse antimicrobial resistance determinants in genetically unrelated biocide tolerant klebsiella pneumoniae. | [this corrects the article doi: 10.1371/journal.pone.0166730.]. | 2017 | 28380066 |
| extended-spectrum β-lactamase-producing klebsiella pneumoniae from pharmaceutical wastewaters in south-western nigeria. | emergence and spread of klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (esbls) present a major threat to public health. in this study, we characterized β-lactam-resistant k. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in lagos and ogun states, nigeria. bacteria were isolated by using macconkey agar; species identification and antibacterial susceptibility testing were performed by vitek 2. etest was used for esbl phe ... | 2017 | 28375698 |
| generation and validation of the ikp1289 metabolic model for klebsiella pneumoniae kppr1. | klebsiella pneumoniae has a reputation for causing a wide range of infectious conditions, with numerous highly virulent and antibiotic-resistant strains. metabolic models have the potential to provide insights into the growth behavior, nutrient requirements, essential genes, and candidate drug targets in these strains. here we develop a metabolic model for kppr1, a highly virulent strain of k. pneumoniae. we apply a combination of biolog phenotype data and fitness data to validate and refine our ... | 2017 | 28375518 |
| clinical implications of genomic adaptation and evolution of carbapenem-resistant klebsiella pneumoniae. | klebsiella pneumoniae poses a major challenge to healthcare worldwide as an important cause of multidrug-resistant infections. nosocomial clones, including epidemic sequence type 258 (st258), have shown an affinity for acquiring and disseminating resistance plasmids, particularly variants of the k. pneumoniae carbapenemase. by comparison, the resurgence of severe community-associated k. pneumoniae infections has led to increased recognition of hypervirulent strains belonging to the k1 and k2 cap ... | 2017 | 28375514 |
| the epidemiology of carbapenem-resistant enterobacteriaceae: the impact and evolution of a global menace. | carbapenem-resistant enterobacteriaceae (cre) are a serious public health threat. infections due to these organisms are associated with significant morbidity and mortality. mechanisms of drug resistance in gram-negative bacteria (gnb) are numerous; β-lactamase genes carried on mobile genetic elements are a key mechanism for the rapid spread of antibiotic-resistant gnb worldwide. transmissible carbapenem-resistance in enterobacteriaceae has been recognized for the last 2 decades, but global disse ... | 2017 | 28375512 |
| aminoglycosides against carbapenem-resistant enterobacteriaceae in the critically ill: the pitfalls of aminoglycoside susceptibility. | the emergence of carbapenem-resistant enterobacteriaceae (cre) has brought aminoglycosides to the frontline since an aminoglycoside may be the only antimicrobial to which cre isolates show in vitro susceptibility. the appropriateness of aminoglycoside-based therapies for severe infections by cre is discussed considering the current breakpoints and recent pharmacokinetic (pk) studies in critically ill patients. areas covered: many aminoglycoside-susceptible cre isolates present minimal inhibitory ... | 2017 | 28375030 |
| virtual screening for potential inhibitors of ctx-m-15 protein of klebsiella pneumoniae. | the gram-negative bacterium klebsiella pneumoniae, responsible for a wide variety of nosocomial infections in immuno-deficient patients, involves the respiratory, urinary and gastrointestinal tract infections and septicemia. extended spectrum β-lactamases (esbl) belong to β-lactamases capable of conferring antibiotic resistance in gram-negative bacteria. ctx-m-15, a prevalent esbl reported from enterobacteriaceae including k. pneumoniae, was selected as a potent anti-bacterial target. to identif ... | 2017 | 28374117 |
| in vitro evaluation of different antimicrobial combinations against carbapenemase-producing klebsiella pneumoniae: the activity of the double-carbapenem regimen is related to meropenem mic value. | we evaluated the in vitro activity of different antimicrobial combinations with and without colistin against 39 carbapenem-resistant klebsiella pneumoniae (cr-kp) strains (colistin + meropenem/doripenem, colistin + tigecycline, colistin + rifampicin, gentamicin + meropenem, gentamicin + tigecycline and the double-carbapenem regimen meropenem + ertapenem) using the chequerboard method. the triple combination colistin + meropenem + tigecycline was also tested. in addition, killing studies were per ... | 2017 | 28369424 |
| fecal microbiota transplantation in patients with blood disorders inhibits gut colonization with antibiotic-resistant bacteria: results of a prospective, single-center study. | patients with blood disorders colonized with antibiotic-resistant bacteria (arb) are prone to systemic infections that are difficult to treat. reintroduction of commensal bacteria in a murine model of enterococcal colonization of the gut can lead to eradication of enterococci. we hypothesized that fecal microbiota transplantation (fmt) could be used to eradicate arb in humans. | 2017 | 28369341 |
| gastrointestinal carriage is a major reservoir of k. pneumoniae infection in intensive care patients. | klebsiella pneumoniae (kp) is an opportunistic pathogen and leading cause of hospital-associated infections. intensive care unit (icu) patients are particularly at risk. kp is part of the healthy human microbiome, providing a potential reservoir for infection. however, the frequency of gut colonization and its contribution to infections are not well characterized. | 2017 | 28369261 |
| insights on klebsiella pneumoniae biofilms assembled on different surfaces using phenotypic and genotypic approaches. | klebsiella pneumoniae is a prominent etiological agent of healthcare associated infections (hais). in this context, multidrug-resistant and biofilm-producing bacteria are of special public health concern due to the difficulties associated with treatment of human infections and eradication from hospital environments. here, in order to study the impact of medical devices-associated materials on the biofilm dynamics, we performed biofilm phenotypic analyses through a classic and a new scanning elec ... | 2017 | 28368366 |
| antimicrobial consumption and antimicrobial resistance: a snapshot of an italian neuromuscular rehabilitation center. | the paper presents a snapshot of the incidence of antimicrobial-resistant microorganisms and antimicrobial consumption in an italian rehabilitation center over a two-year period (2014-2015). data on microorganism identification and antimicrobial susceptibility testing were obtained from the diagnostic laboratory of the hospital. a set of indicators was assessed, including the incidence density of resistant isolates per 1000 patient-days (idri). data on antimicrobial consumption, semi-annually, o ... | 2017 | 28368076 |
| management of carbapenem-resistant k. pneumoniae in allogenic stem cell transplant recipients: the turin bundle. | carbapenem resistance has evolved rapidly since 2001 and the distribution of carbapenemase-producing klebsiella pneumoniae (cr-kp) is currently a public health concern worldwide. in the haematological setting, especially in allogenic transplant, cr-kp infections were associated with a mortality up to 65%. aim of this report is to describe the management of patients colonized by cr-kp and undergoing allo-hsct with a multiple-step intervention strategy: the "turin bundle". steps included oral gent ... | 2017 | 28368074 |
| molecular analysis of the ramra locus in clinical klebsiella pneumoniae isolates with reduced susceptibility to tigecycline. | mutations in ramr, a negative regulator of rama which stimulates transcription of acra/-b encoding the multidrug efflux pump acrab-tolc, were recently shown to result in reduced susceptibility to tigecycline in klebsiella pneumoniae. we analysed six non-duplicate k. pneumoniae isolates with elevated mics to tigecycline. all isolates showed transcriptional up-regulation of rama and acrb as demonstrated by northern blot and quantitative real-time pcr. sequencing of the ramr gene revealed deletions ... | 2017 | 28368073 |
| high production of 2,3-butanediol by a mutant strain of the newly isolated klebsiella pneumoniae srp2 with increased tolerance towards glycerol. | biodiesel, a renewable fuel produced by transesterification of animal fats and vegetable oils, generates about 10% (v/v) of crude glycerol as a core byproduct. the high volume of this non bio-degradable glycerol is becoming of a great environmental and economical concern due to its worldwide ever-growing surplus. herein we report a high production of 2,3-butanediol (2,3-bd) from pure and biodiesel derived crude glycerol using a mutant k. pneumoniae srm2 obtained from a newly isolated strain kleb ... | 2017 | 28367095 |
| biofilm formation of hypermucoviscous and non-hypermucoviscous klebsiella pneumoniae recovered from clinically affected african green monkey (chlorocebus aethiops sabaeus). | in recent years, an emergent klebsiella pneumoniae hypermucoviscous (hmv) phenotype has been associated with increased invasiveness and pathogenicity in primates. the hmv phenotype is characterized by different capsular serotypes, associated with several genes including the rmpa (regulator of mucoid phenotype) and maga (mucoviscosity-associated) genes. in african green monkeys (agm) (chlorocebus aethiops sabaeus) serotypes k1 and k5 have been implicated in fatal multisystemic abscesses. in order ... | 2017 | 28366827 |
| evolution and transmission of carbapenem-resistant klebsiella pneumoniae expressing the blaoxa-232 gene during an institutional outbreak associated with endoscopic retrograde cholangiopancreatography. | whole-genome sequencing (wgs) is an emerging and powerful technique by which to perform epidemiological studies in outbreak situations. | 2017 | 28362935 |
| complete genome sequence of carbapenem-resistant klebsiella pneumoniae strain 1756, isolated from a pus specimen. | carbapenem-resistant klebsiella pneumoniae strain 1756 was isolated from a pus specimen from a taiwanese patient. here, the complete genome sequence of strain 1756 is presented. | 2017 | 28360152 |
| systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant klebsiella pneumoniae. | carbapenem resistant k. pneumoniae (crkp) has aroused widespread attention owing to its very limited therapeutic options, and this strain has increased rapidly in recent years. although it is accepted that drug resistance is associated with increased mortality in general, but some other studies found no such relationship. to estimate mortality of patients infected with crkp in general and analyze factors for mortality of this infection, thus, we conducted this systematic review and meta-analysis ... | 2017 | 28356109 |
| enterobacteriaceae producing extended-spectrum β-lactamases (esbls) colonization as a risk factor for developing esbl infections in pediatric cardiac surgery patients: "retrospective cohort study". | children with cardiac defects need many hospitalizations and repetitive antibiotic therapies, with an increasing risk of colonization with multidrug-resistant bacteria (mdrb) such as extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) post-operative infections with these bacteria in paediatric cardiac surgery are life threatening. this article aims to study the prevalence of esbl colonization among paediatric cardiac surgery patients, and to compare occurrence of post-operative i ... | 2017 | 28356079 |
| simultaneous detection of nine key bacterial respiratory pathogens using luminex xtag(®) technology. | early diagnosis and treatment are crucial to the outcome of lower respiratory tract infections (lrtis). in this study, we developed an assay combining multiplex pcr and luminex technology (mplt) for the detection of nine important respiratory bacterial pathogens, which frequently cause lrtis. these were streptococcus pneumoniae, moraxella catarrhalis, staphylococcus aureus, streptococcus pyogenes, haemophilus influenzae, mycoplasma pneumoniae, legionella spp., pseudomonas aeruginosa, and klebsie ... | 2017 | 28241513 |
| bacterial and viral pathogen spectra of acute respiratory infections in under-5 children in hospital settings in dhaka city. | the study aimed to examine for the first time the spectra of viral and bacterial pathogens along with the antibiotic susceptibility of the isolated bacteria in under-5 children with acute respiratory infections (aris) in hospital settings of dhaka, bangladesh. nasal swabs were collected from 200 under-five children hospitalized with clinical signs of aris. nasal swabs from 30 asymptomatic children were also collected. screening of viral pathogens targeted ten respiratory viruses using rt-qpcr. b ... | 2017 | 28346512 |
| a thermostabilized, one-step pcr assay for simultaneous detection of klebsiella pneumoniae and haemophilus influenzae. | klebsiella pneumoniae and haemophilus influenzae are two common pathogens associated with respiratory tract infections. the identification of these pathogens using conventional molecular diagnostic tests requires trained personnel, cold-chain transportation, and storage-dependance, which does not render them user-friendly. the aim of this study was to develop a thermostabilized, cold-chain-free, one-step multiplex pcr for simultaneous detection of k. pneumoniae and h. influenzae. the multiplex p ... | 2017 | 28386286 |
| neonatal sepsis in haiti. | infections (including sepsis, meningitis, pneumonia and tetanus) stand as a major contributor to neonatal mortality in haiti (22%). infants acquire bacteria that cause neonatal sepsis directly from the mother's blood, skin or vaginal tract either before or during delivery. nosocomial and environmental pathogens introduce further risk after delivery. the absence of cohesive medical systems and methods for collecting information limits the available data in countries such as haiti. this study seek ... | 2017 | 27765888 |
| community-onset carbapenem-resistant klebsiella pneumoniae urinary tract infections in infancy following nicu hospitalisation. | urinary tract infection (uti) is a common bacterial infection in childhood with favourable outcome. however, the recent emergence of uti caused by multidrug-resistant pathogens, such as carbapenem-resistant enterobacteriaceae (cre), has become a great concern worldwide. cre are mainly responsible for nosocomial infections and community-onset cre infections in healthy individuals are rare. | 2017 | 28341427 |
| first report of oxa-232-producing klebsiella pneumoniae strains in tunisia. | eleven carbapenem-resistant klebsiella pneumoniae isolates were recovered from the military hospital of tunis, tunisia. three of these isolates carried the carbapenemase blaoxa-232 gene recently identified in france. these isolates were clonally related and co-expressed the extended-spectrum ß-lactamase, ctx-m-15. this work identified the occurrence of oxa-232 producers in north-african countries. | 2017 | 28341097 |
| the hypervirulent variant of klebsiella pneumoniae is established in new york city. | 2017 | 28339856 | |
| defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of klebsiella pneumoniae. | at present, the most used methods for klebsiella pneumoniae subtyping are multilocus sequence typing (mlst) and pulsed-field gel electrophoresis (pfge). however, the discriminatory power of mlst could not meet the need for distinguishing outbreak and non-outbreak isolates and the pfge is time-consuming and labor-intensive. a core genome multilocus sequence typing (cgmlst) scheme for whole-genome sequence-based typing of k. pneumoniae was developed for solving the disadvantages of these tradition ... | 2017 | 28337187 |
| draft genome sequence of klebsiella pneumoniae subsp. pneumoniae atcc 9621. | we present here the 5.561-mbp assembled draft genome sequence of klebsiella pneumoniae subsp. pneumoniae atcc 9621, a phosphite- and organophosphonate-assimilating gammaproteobacterium. the genome harbors 5,179 predicted protein-coding genes. | 2017 | 28336608 |
| first detection of klebsiella pneumoniae producing oxa-48 in fresh vegetables from béjaïa city, algeria. | 2017 | 28336324 | |
| genome sequencing and comparative analysis of three hypermucoviscous klebsiella pneumoniae strains isolated in russia. | the prevalence and characteristics of hypermucoviscous (hv) strains among klebsiella pneumoniae isolated in russian hospitals were investigated. the hv strains were accounted for 11% k. pneumoniae isolates collected on the period from 2011 to 2016, and were characterized by belonging to k1, k2, k20 and k57 serotypes. whole genome sequences (wgss) of k. pneumoniae hv clinical strains kpi261 (scpm-o-b-7850) and kpb4010 (scpm-o-b-7846) belonging to k1 and k2 capsular types, as well as wgss of k. pn ... | 2017 | 28334288 |
| multi-scale model of drug induced adaptive resistance of gram-negative bacteria to polymyxin b. | the purpose of this report is to apply multi-scale modeling using the theory of physiologically structured populations (psp) to develop a mathematical model for antimicrobial resistance based on a heterogeneous distribution of receptors and affinities among bacterial cells. the theory has been tested on data obtained from an in vitro static time-kill infection model analyzing the pharmacodynamics of polymyxin b against gram-negative bacteria. the drug binding parameter kd (dissociation equilibri ... | 2017 | 28334005 |
| high ceftazidime hydrolysis activity and porin ompk35 deficiency contribute to the decreased susceptibility to ceftazidime/avibactam in kpc-producing klebsiella pneumoniae. | to investigate mechanisms for the decreased susceptibility to ceftazidime/avibactam in kpc-producing klebsiella pneumoniae (kpc-kp). | 2017 | 28333323 |
| identifying the shared metabolic objectives of glycerol bioconversion in klebsiella pneumoniae under different culture conditions. | this paper addresses the problem of identifying the shared metabolic objectives of glycerol bioconversion in klebsiella pneumoniae for production of 1,3-propanediol (1,3-pd) under different culture conditions. to achieve this goal, we propose a multi-level programming model. this model includes three optimization problems, where the constraint region of the first level problem is implicitly determined by the other two optimization problems. the optimized objectives of the first and second level ... | 2017 | 28323016 |
| neonatal cholestasis mimicking biliary atresia: could it be urinary tract infection? | cholestasis can occur in newborns due to infections. however, the manifestations of the underlying infections usually dominate the presentation. we present a 2-month-old infant who presented with jaundice and no fever or signs of systemic illness. liver biopsy was suggestive of cholangitis. he was subsequently detected to have urinary tract infection with klebsiella pneumoniae. the child was treated with appropriate antibiotics for 2 weeks following which the cholestasis resolved. thus, neonatal ... | 2017 | 28321310 |
| infections in patients with a total artificial heart are common but rarely fatal. | patients who received a total artificial heart (tah) at virginia commonwealth university (vcu) between january 1, 2010 and december 31, 2011 were identified from the vcu mechanical circulatory support clinical database. retrospective data extraction from the medical records was performed from the time of tah implantation until heart transplantation or death. infections were classified as confirmed or suspected. twenty-seven men and 5 women, mean age 49.5 years (range 24 to 68 years) received a t ... | 2017 | 28319520 |
| expression of 16 nitrogenase proteins within the plant mitochondrial matrix. | the industrial production and use of nitrogenous fertilizer involves significant environmental and economic costs. strategies to reduce fertilizer dependency are required to address the world's increasing demand for sustainable food, fibers, and biofuels. biological nitrogen fixation, a process unique to diazatrophic bacteria, is catalyzed by the nitrogenase complex, and reconstituting this function in plant cells is an ambitious biotechnological strategy to reduce fertilizer use. here we establ ... | 2017 | 28316608 |
| emergence of st39 and st656 extensively drug-resistant klebsiella pneumoniae isolates in wenzhou, china. | 2017 | 28303839 | |
| carbapenemases-producing klebsiella pneumoniae in hospitals of two regions of southern italy. | carbapenem-resistant klebsiella pneumoniae infections are reported with increasing frequency elsewhere in the world, representing a worrying phenomenon for global health. in italy, there are hotspot data on the diffusion and type of carbapenemase-producing enterobacteriaceae and k. pneumoniae in particular, with very few data coming from apulia and basilicata, two regions of southern italy. this study was aimed at characterizing by phenotypic and genotypic methods carbapenem-resistant k. pneumon ... | 2017 | 28295617 |
| detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of klebsiella pneumoniae. | carbapenems are considered last-line agents for the treatment of serious infections caused by klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. we evaluated 27 isolates of k. pneumoniae resistant to carbapenems recovered from inpatients at the university hospital of santa maria-rs from july 2013 to august 2014. we carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of eff ... | 2017 | 28291698 |
| molecular characterization of kpc-producing klebsiella pneumoniae isolated from patients in a public hospital in caracas, venezuela. | klebsiella pneumoniae carbapenemase (kpc)-producing bacteria are amongst the most important causative agents of nosocomial infections worldwide. isolates of this bacterium have been identified in venezuela but little is known about their local spread. the aim of this study was to perform the molecular characterization of kpc-producing strains isolated from 2012 to 2013 in a public hospital in caracas, venezuela. | 2017 | 28291671 |
| discovery of novel pyridone-conjugated monosulfactams as potent and broad-spectrum antibiotics for multidrug-resistant gram-negative infections. | conjugating a siderophore to an antibiotic is a promising strategy to overcome the permeability-mediated resistance of gram-negative pathogens. on the basis of the structure of bal30072, novel pyridone-conjugated monosulfactams incorporating diverse substituents into the methylene linker between the 1,3-dihydroxypyridin-4(1h)-one and the aminothiazole oxime were designed and synthesized. structure-activity relationship studies revealed that a variety of substituents were tolerated, with isopropy ... | 2017 | 28287720 |
| disparate domains: klebsiella pneumoniae liver abscess syndrome. | 2017 | 28286165 | |
| first report of colistin-resistant klebsiella pneumoniae clinical isolates in lebanon. | 2017 | 28286141 | |
| more than meets the eye: klebsiella pneumoniae invasive liver abscess syndrome presenting with endophthalmitis. | endophthalmitis is a feared complication of pyogenic liver abscesses caused by hypervirulent klebsiella pneumoniae strains. first described in east asia in the 1980s, this invasive syndrome is only recently emerging in europe and america. | 2017 | 28285868 |