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feasibility of radiofrequency ablation as an alternative to surgical intervention in patients with huge multiloculated pyogenic liver abscesses: a retrospective cohort study.this retrospective cohort study investigated the feasibility of radiofrequency (rf) ablation as an alternative to surgical intervention in patients with huge multiloculated pyogenic liver abscesses (plas).from august 2010 to april 2016, 83 patients with pla were admitted to beijing chao-yang hospital, china. four of these patients had huge multiloculated plas and underwent rf ablation plus antibiotics. the inclusion criteria for rf ablation were as follows: multiloculated pla confirmed by comput ...201627930527
a retrospective study of pyogenic liver abscess focusing on klebsiella pneumoniae as a primary pathogen in china from 1994 to 2015.pyogenic liver abscess (pla) is a common intra-abdominal infection in adults. in this study, we aim to explore demographic and clinical characteristics of pla focusing on klebsiella pneumoniae (k. pneumoniae) induced pla (kp-pla) in mainland china. a retrospective review of medical records from all patients with kp-pla admitted to a tertiary teaching hospital over a 21-year period (1994-2015) was performed. among 296 pla cases with confirmed culture-positive data, k. pneumoniae was revealed as t ...201627929082
ventriculostomy-related infections in subarachnoid hemorrhage patients-a retrospective study of incidence, etiology, and antimicrobial therapy.this study was performed to investigate the incidence and etiology of ventriculostomy-related infections (vris) in patients with subarachnoid hemorrhage (sah) and to assess adherence to local clinical guidelines regarding empirical antimicrobial therapy and diagnostic routines.201727928632
antimicrobial resistance temporal trend of klebsiella pneumoniae isolated from blood.background. according to the european antimicrobial resistance surveillance network, romania reports an increasing number of resistant klebsiella pneumoniae strains from invasive infections every year. material and method. we analyzed the antimicrobial susceptibility of klebsiella pneumoniae strains isolated from blood in 2010 and 2015 in "matei bals" national institute of infectious diseases, in order to identify any significant changes in the last five years. results. we identified 18 strains ...201727928448
phenotypic and molecular detection of the bla kpc gene in clinical isolates from inpatients at hospitals in são luis, ma, brazil.bacteria that produce klebsiella pneumoniae carbapenemases (kpcs) are resistant to broad-spectrum β-lactam antibiotics. the objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in são luis (maranhão, brazil).201627927163
antioxidant, anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica.stachys guyoniana noë ex. batt. and mentha aquatica l. are two algerian lamiaceae used in folk medicine.201727927090
multidrug resistant enterobacteriaceae in new zealand: a current perspective.in this article we review mechanisms and potential transmission pathways of multidrug resistance in enterobacteriaceae, with an emphasis on extended-spectrum β-lactamase (esbl)-production. this provides background to better understand challenges presented by this important group of antimicrobial resistant bacteria, and inform measures aimed at prevention and control of antimicrobial resistance in general. humans and animals interact at various levels; household pets cohabit with humans, and othe ...201727927087
the metabolic flux regulation of klebsiella pneumoniae based on quorum sensing system.quorum-sensing (qs) systems exist universally in bacteria to regulate multiple biological functions. klebsiella pneumoniae, an industrially important bacterium that produces bio-based chemicals such as 2,3-butanediol and acetoin, can secrete a furanosyl borate diester (ai-2) as the signalling molecule mediating a qs system, which plays a key regulatory role in the biosynthesis of secondary metabolites. in this study, the molecular regulation and metabolic functions of a qs system in k. pneumonia ...201627924940
il-17 receptor signaling in the lung epithelium is required for mucosal chemokine gradients and pulmonary host defense against k. pneumoniae.the cytokine il-17, and signaling via its heterodimeric il-17ra/il-17rc receptor, is critical for host defense against extracellular bacterial and fungal pathogens. polarized lung epithelial cells express il-17ra and il-17rc basolaterally. however, their contribution to il-17-dependent pulmonary defenses in vivo remains to be determined. to address this, we generated mice with conditional deletion of il17ra or il17rc in scgb1a1-expressing club cells, a major component of the murine bronchiolar e ...201627923703
outbreak of klebsiella pneumoniae carbapenemase-producing citrobacter freundii at a tertiary acute care facility in miami, florida.objective to describe the investigation and control of a rare cluster of klebsiella pneumoniae carbapenemase-producing citrobacter freundii in a hospital in southern florida. methods an epidemiologic investigation, review of infection prevention procedures, and molecular studies including whole genome sequencing were conducted. results an outbreak of k. pneumoniae carbapenemase-3-producing c. freundii was identified at a tertiary hospital in florida in 2014. of the 6 cases identified, 3 occurred ...201727923418
community-acquired meningitis caused by a cg86 hypervirulent klebsiella pneumoniae strain: first case report in the caribbean.community-acquired bacterial meningitis due to klebsiella pneumoniae has mainly been described in southeast asia and has a poor prognosis. severe invasive infections caused by k. pneumoniae, including meningitis, are often due to hypervirulent strains (hvkp), which are characterized by capsular serotypes k1 and k2, a gene responsible for hypermucoviscosity, and the cluster for synthesis of the siderophore aerobactin.201627923372
extended spectrum cephalosporin resistance among clinical isolates of enterobacteriaceae in west norway during 2006-2013; a prospective surveillance study.routine surveillance of resistance to broad-spectrum cephalosporins in enterobacteriaceae and phenotypic identification of underlying mechanisms using a simple strategy was commenced in 2006 at our laboratory, serving west norway. this report focuses on the results until 2013. the classical plasmid-mediated extended spectrum beta-lactamase (esbla ) among clinically relevant escherichia coli isolates showed an increase from 0.6% to 4.3% during the surveillance period, while prevalence for other m ...201727921337
genetic environment of the blakpc-2 gene in a klebsiella pneumoniae isolate that may have been imported to russia from southeast asia.the nucleotide sequence of a blakpc-2-harboring plasmid (pkpcapss) from klebsiella pneumoniae st273 isolated in saint petersburg, russia, from a patient with history of recent travel to vietnam is presented. this 127,970-bp plasmid possessed both incfii and incr replicons. blakpc-2 was localized on a hypothetical mobile element. this element was flanked by 38-bp inverted tn3 repeats and included a tn3-specific transposase gene, macrolide resistance operon (mpha-mrx-mphr), and a fragment of blate ...201727919902
a prolonged outbreak of kpc-3-producing enterobacter cloacae and klebsiella pneumoniae driven by multiple mechanisms of resistance transmission at a large academic burn center.klebsiella pneumoniae carbapenemase (kpc)-producing enterobacter cloacae has been recently recognized in the united states. whole-genome sequencing (wgs) has become a useful tool for analysis of outbreaks and for determining transmission networks of multidrug-resistant organisms in health care settings, including carbapenem-resistant enterobacteriaceae (cre). we experienced a prolonged outbreak of cre e. cloacae and k. pneumoniae over a 3-year period at a large academic burn center despite rigor ...201727919898
refugees and antimicrobial resistance: a systematic review.there is a large increase in the numbers of refugees and asylum seekers worldwide and a lack of data on the carriage of antimicrobial resistance in refugee/asylum seeking groups.201727919742
[rapid test for detection of susceptibility to cefotaxime in enterobacteriaceae].in this work an "in house" rapid test based on the change in ph that is due to hydrolysis for detecting enterobacteriaceae susceptible to cefotaxime is evaluated. the strains of enterobacteriaceae from 1947 urine cultures were assessed using microscan panels and the "in house" test. this rapid test includes red phenol solution and cefotaxime. using microscan panels, 499 enterobacteriaceae isolates were evaluated, which included 27 isolates of escherichia coli producing extended-spectrum beta-lac ...201627919513
chemical profiling, antimicrobial and insecticidal evaluations of polygonum hydropiper l.the emergence of multidrug resistant (mdr) pathogens is of great concern to the global health community. our ability to effectively treat diseases is based on the discovery of potent drugs for the treatment of these challenging diseases. traditional medicines are one of the major sources for the discovery of safe, effective and economical drug candidates. in order to validate its antibacterial, antifungal and insecticidal potentials with respect to traditional uses, we have screened for the firs ...201627919287
metabolic engineering of corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose.3-hydroxypropionic acid (3-hp) is a promising platform chemical which can be used for the production of various value-added chemicals. in this study,corynebacterium glutamicum was metabolically engineered to efficiently produce 3-hp from glucose and xylose via the glycerol pathway. a functional 3-hp synthesis pathway was engineered through a combination of genes involved in glycerol synthesis (fusion of gpd and gpp from saccharomyces cerevisiae) and 3-hp production (pducdegh from klebsiella pneu ...201727918882
the crystal structure of the endoglucanase cel10, a family 8 glycosyl hydrolase from klebsiella pneumoniae.cellulases are produced by microorganisms that grow on cellulose biomass. here, a cellulase, cel10, was identified in a strain of klebsiella pneumoniae isolated from chinese bamboo rat gut. analysis of substrate specificity showed that cel10 is able to hydrolyze amorphous carboxymethyl cellulose (cmc) and crystalline forms of cellulose (avicel and xylan) but is unable to hydrolyze p-nitrophenol β-d-glucopyranoside (p-npg), proving that cel10 is an endoglucanase. a phylogenetic tree analysis indi ...201627917834
once daily high dose tigecycline - pharmacokinetic/pharmacodynamic based dosing for optimal clinical effectiveness: dosing matters, revisited.tigecycline has emerged as first line therapy for serious systemic infections due to important pathogens (except p. aeruginosa and proteus sp.), including multi-drug resistant (mdr) and gram negative bacilli (gnb), including carbapenem resistant enterobacteriae. tigecycline has a 'low resistance potential,' is protective against c. difficile, and is often the only antibiotic effective against mdr gnb, e.g., klebsiella sp. areas covered: standard dose tigecycline therapy has been used for intra-a ...201727917692
synergistic effect of thymbra spicata l. extracts with antibiotics against multidrug- resistant staphylococcus aureus and klebsiella pneumoniae strains.to evaluate the in vitro interaction between different extracts of thymbra spicata l. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. this study was performed against multidrug-resistant strains of staphylococcus aureus and klebsiella pneumoniae.201627917275
capsule-targeting depolymerase, derived from klebsiella kp36 phage, as a tool for the development of anti-virulent strategy.the rise of antibiotic-resistant klebsiella pneumoniae, a leading nosocomial pathogen, prompts the need for alternative therapies. we have identified and characterized a novel depolymerase enzyme encoded by klebsiella phage kp36 (depokp36), from the siphoviridae family. to gain insights into the catalytic and structural features of depokp36, we have recombinantly produced this protein of 93.4 kda and showed that it is able to hydrolyze a crude exopolysaccharide of a k. pneumoniae host. using in ...201627916936
[experience of the selection and maintainence for donor lung: 62 cases attached].objective: to summarize the experience of selection and maintainence for the lungs from donation. methods: from january 2015 to june 2016, 62 cases had been evaluated by department of thoracic surgery, first affiliated hospital of guangzhou medical university. all the clinic data had been collected to evaluate the donor lungs, including the basic information, physical examination, results of radiology and laboratory, the bronchoscope, etc. all the satisfactory donors had been harvested and trans ...201627916031
efflux mediated colistin resistance in diverse clones of klebsiella pneumoniae from aquatic environment.the peptide drug colistin is commonly used to treat carbapenem resistant gram negative bacterial infections. in the present study, we report efflux mediated colistin resistance in multidrug resistant klebsiella pneumoniae isolates belonging to st200 and st1296, isolated from a fresh water environment. the isolates exhibited intermediate resistance to human serum, possessed type 1 fimbriae and harbored blashv-34 and blatem-1 genes. our results highlight the evolving nature of these clones in the ...201727914962
primary liver abscess caused by klebsiella pneumoniae: an increasingly common entity. 201627914678
biofilm prevention of gram-negative bacterial pathogens involved in periprosthetic infection by antibiotic-loaded calcium sulfate beads in vitro.biofilm formation represents a key stage in the pathogenesis of prosthetic infections (pis). more tolerant to antibiotics than their planktonic counterparts, biofilm bacteria are difficult to eradicate using conventional therapeutic regimes. a common approach in pi management is the adjunctive use of localised antibiotics in addition to systemic administration in an attempt to protect the implant from colonisation by infiltrating bacteria. this study evaluates the antibacterial and antibiofilm e ...201627910828
previous bloodstream infections due to other pathogens as predictors of carbapenem-resistant klebsiella pneumoniae bacteraemia in colonized patients: results from a retrospective multicentre study.the purpose of this retrospective multicenter study was to assess whether the risk of developing bloodstream infections (bsi) due to carbapenem-resistant klebsiella pneumoniae (crkp) in colonized patients is influenced by the occurrence of bsi due to other pathogens.201727909821
trends in carbapenem susceptibility of acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae isolates from long-term acute care hospitals across the united states-an analysis of antibiograms. 201527909697
for reliable urine cultures in the detection of complicated urinary tract infection, do we use urine specimens obtained with urethral catheter or a nephrostomy tube?the aim of this study was to compare the results of urine cultures obtained either from urethral, and percutaneous nephrostomy (pcn) catheters.201627909624
nitrobacter sp. extract mediated biosynthesis of ag2o nps with excellent antioxidant and antibacterial potential for biomedical application.in this study, extracellular extract of plant growth promoting bacterium, nitrobacter sp. is used for the bioconversion of agno3 (silver nitrate) into ag2o (silver oxide nanoparticles). it is an easy, ecofriendly and single step method for ag2o nps synthesis. the bio-synthesized nanoparticles were characterized using different techniques. uv-vis results showed the maximum absorbance around 450 nm. xrd result shows the particles to have faced centered cubic (fcc) crystalline nature. ftir analysis ...201627906145
myxobacteria-mediated synthesis of silver nanoparticles and their impregnation in wrapping paper used for enhancing shelf life of apples.the authors report myxobacteria virescens (m. virescens) mediated synthesis of silver nanoparticles (agnps) and its efficacy against staphylococcus aureus (atcc-33591), salmonella typhi (atcc-51812), escherichia coli (e. coli) (atcc-14948), klebsiella pneumoniae (mtcc-4030) and pseudomonas aeruginosa (mtcc-4673). the organism exhibiting resistance to various antibiotics showed remarkable sensitivity, when used in combination of antibiotics and agnps. antimicrobial property of agnps is playing a ...201627906139
rapid synthesis of silver nanoparticles by pseudomonas stutzeri isolated from textile soil under optimised conditions and evaluation of their antimicrobial and cytotoxicity properties.present study utilised textile soil isolated bacterium pseudomonas stutzeri to synthesise extracellular silver nanoparticles (agnps) under optimised conditions. the synthesised agnps were characterised using ultraviolet-visible spectroscopy, fourier transform infrared spectroscopy (ftir) and transmission electron microscopy (tem). optimisation showed agnps synthesis within 8 h using 2mm ag nitrate at ph9, temperature 80°c and maximum absorbance toward 400 nm. tem analysis revealed spherical shap ...201627906136
broad-spectrum of antimicrobial properties of commercial wines from different vitis vinifera l. varieties.fifteen commercial wines produced from international and autochthonic varieties of vitis vinifera l. cultivation of different balkan winegrowing subregions were studied for their antimicrobial activity against six gram-positive (clostridium perfringens, bacillus subtilis, staphylococcus aureus, listeria inocua, sarcina lutea and micrococcus flavus) and six gram-negative (escherichia coli, pseudomonas aeruginosa, salmonella enteritidis, shigella sonnei, klebsiella pneumonia and proteus vulgaris) ...201727905092
central-line associated bloodstream infections in a tertiary care children's university hospital: a prospective study.the central-line associated bloodstream infections (clabsi) are the most common healthcare-associated infections in childhood. despite the international data available on healthcare-associated infections in selected groups of patients, there is a lack of large and good quality studies. the present survey is the first prospective study monitoring for 6 months the occurrence of central-line associated bloodstream infections in all departments of an italian tertiary care children's university hospi ...201627903240
risk factors and mortality in the carbapenem-resistant klebsiella pneumoniae infection: case control study.carbapenem-resistant klebsiella pneumoniae (crkp) has been known as a nosocomial pathogen, both for the last 10 years in turkey and for 20 years worldwide. due to limited treatment options and high mortality rates, despite improvements in the field of medicine at the present time, crkp is still a big threat for public health. this study was carried out between the dates of january 2010 and september 2014. patients ≥18 who were hospitalized for at least 72 h and who also had crkp growth were incl ...201727903130
ultrastructural changes caused by polymyxin b and meropenem in multiresistant klebsiella pneumoniae carrying blakpc-2 gene.the ultrastructural alterations caused by polymyxin b and meropenem and by the association between polymyxin b and meropenem were investigated in two multiresistant isolates of klebsiella pneumoniae (k3-a2 and k12-a2) carriers of blakpc-2, coming from infection and colonization in patients in a public hospital in recife, brazil. the ultrastructural changes were detected by transmission electron microscopy and scanning. the susceptibility of the isolates to antimicrobials was tested by the disc d ...201627902404
involvement of camp receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of klebsiella pneumoniae serotype k1 causing pyogenic liver abscess.the global regulator camp receptor protein (crp) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. in this work, the crp mutant as well as the complemented mutant was constructed from a wild-type klebsiella pneumoniae capsular serotype k1 strain causing the primary pyogenic liver abscess. the phenotypes of wild-type strain, crp mutant and complemented mutant were characterized systematically. it was disc ...201727902401
detection of escherichia coli st131 clonal complex (st705) and klebsiella pneumoniae st15 among faecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae.the objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-e) and carbapenemase-producing enterobacteriaceae (cpe) in non-selected hospitalized and non-hospitalized patients from the same geographic area of madrid.201727902381
synthesis and antimicrobial evaluation of ester-linked 1,4-disubstituted 1,2,3-triazoles with a furyl/thienyl moiety.twenty ester-linked 1,4-disubstituted 1,2,3-triazoles having a furyl/thienyl moiety have been synthesized from heteroaryl prop-2-yn-1-yl carboxylate and aromatic azides via a cu(i) catalyzed 1,3-dipolar cycloaddition. all the synthesized compounds were characterized by ftir, [formula: see text]h nmr, [formula: see text]c nmr spectroscopy and hrms. synthesized triazoles were tested in vitro for antimicrobial evaluation against gram-negative bacteria-escherichia coli, enterobacter aerogenes and kl ...201727900513
assessment of the activity of tigecycline against gram-positive and gram-negative organisms collected from italy between 2012 and 2014, as part of the tigecycline evaluation and surveillance trial (t.e.s.t.).as part of the tigecycline evaluation and surveillance trial (t.e.s.t) we report the in vitro activity of tigecycline and its comparators against gram-negative and gram-positive organisms collected from italian centers between 2012 and 2014. minimum inhibitory concentrations were determined according to the broth microdilution methodology of the clinical and laboratory standards institute, and antimicrobial resistance was determined using the european committee on antimicrobial susceptibility te ...201627898030
[evaluation of an immunochromatographic test for the detection of oxa-48 carbapenemase].detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. oxa-48 is the most common carbapenemase in spain and in our environment. the aim of this study is the evaluation of a new immunochromatographic test oxa-48 card letitest (coris, bioconcept belgium) to detect this carbapenemase from solid media.201727897435
impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo.short chain fatty acids (scfas) produced by intestinal microbes mediate anti-inflammatory effects, but whether they impact on antimicrobial host defenses remains largely unknown. this is of particular concern in light of the attractiveness of developing scfa-mediated therapies and considering that scfas work as inhibitors of histone deacetylases which are known to interfere with host defenses. here we show that propionate, one of the main scfas, dampens the response of innate immune cells to mic ...201627897220
high prevalence of esbl-producing klebsiella pneumoniae causing community-onset infections in china.the aim of this work was to investigate the epidemiological and genetic characteristics of esbl-producing klebsiella pneumoniae (esbl-kp) causing community-onset infections. k. pneumoniae isolates were collected from 31 chinese secondary hospitals between august 2010 and 2011. genes encoding esbl and ampc beta-lactamases were detected by pcr. the isolates were assigned to sequence types (sts) using multi-locus sequence typing (mlst). eleven esbl-kp strains were selected for whole-genome sequenci ...201627895637
a prospective, observational study comparing the pk/pd relationships of generic meropenem (mercide(®)) to the innovator brand in critically ill patients.clinicians' skepticism, fueled by evidence of inferiority of some multisource generic antimicrobial products, results in the underutilization of more cost-effective generics, especially in critically ill patients. the aim of this observational study was to demonstrate equivalence between the generic or comparator brand of meropenem (mercide(®)) and the leading innovator brand (meronem(®)) by means of an ex vivo technique whereby antimicrobial activity is used to estimate plasma concentration of ...201627895516
the pathogenic potential of proteus mirabilis is enhanced by other uropathogens during polymicrobial urinary tract infection.urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as proteus mirabilis and providencia stuartii, commonly occurs with long-term catheterization. we previously demonstrated that coinfection with p. mirabilis and p. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (uti). in this study, we expanded these findings to a murine model of catheter-associated uti (cauti ...201727895127
incp plasmid carrying colistin resistance gene mcr-1 in klebsiella pneumoniae from hospital sewage.a klebsiella pneumoniae strain of sequence type 313 (st313) recovered from hospital sewage was found carrying the plasmid-borne colistin resistance gene mcr-1, which was bracketed by two copies of the insertion sequence isapl1 on a 57-kb self-transmissible incp-type plasmid of a new incp-1 clade. the carriage of mcr-1 on a self-transmissible broad-host-range plasmid highlights that mcr-1 has the potential to spread beyond the enterobacteriaceae family.201727895009
antibody-free detection of infectious bacteria using quantum dots-based barcode assay.staphylococcus aureus, methicillin-resistant staphylococcus aureus and klebsiella pneumoniae are the most representative bacteria causing infectious diseases. due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. therefore, a sensitive determination of these pathogens is crucial for effective treatment. the aim of this study was to design an effective method for multiplex detection of staphylococcus aureus, methicillin-resistant staphy ...201727894780
epidemiology of klebsiella pneumoniae bloodstream infections in a teaching hospital: factors related to the carbapenem resistance and patient mortality.although klebsiella pneumoniae bloodstream infections (kp-bsis) have recently attracted attention due to an alarming raise in morbidity and mortality, there have been few reports on the epidemiology of kp-bsis in mainland china. we sought to describe the epidemiological, microbiological, and clinical characteristics of kp-bsis, focusing on the risk factors of carbapenem resistance and patient mortality.201627891222
colistin in pig production: chemistry, mechanism of antibacterial action, microbial resistance emergence, and one health perspectives.colistin (polymyxin e) is one of the few cationic antimicrobial peptides commercialized in both human and veterinary medicine. for several years now, colistin has been considered the last line of defense against infections caused by multidrug-resistant gram-negative such as acinetobacter baumannii, pseudomonas aeruginosa, and klebsiella pneumoniae. colistin has been extensively used orally since the 1960s in food animals and particularly in swine for the control of enterobacteriaceae infections. ...201627891118
evaluating the effect of oxygen concentrations on antibiotic sensitivity, growth, and biofilm formation of human pathogens.standard antimicrobial susceptibility tests are performed in vitro under normal room oxygen conditions to predict the in vivo effectiveness of antimicrobial therapy. the aim of this study was to conduct a comprehensive analysis of the effect of different oxygen levels on the antibiotic susceptibility of two strains of staphylococcus aureus, pseudomonas aeruginosa, and klebsiella pneumoniae. it was found that anoxic conditions caused reduced sensitivity of bacteria to aminoglycoside antibiotics i ...201627891050
investigation of carbapenem resistance and the first identification of klebsiella pneumoniae carbapenemase (kpc) enzyme among escherichia coli isolates in turkey: a prospective study.the aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in escherichia coli isolates from clinical samples in turkey.201727890667
peripartum maternal transmission of extended-spectrum β-lactamase organism to newborn infants.the aim of this study was to determine whether the route of extended-spectrum β-lactamase (esbl) transmission to hospitalized newborns was from their mothers during delivery. neonatal intensive care unit (nicu) hospitalized newborns were sampled for esbl presence by stool cultures on the first and fourth days of life. mothers of esbl-positive newborns were sampled for possible correlation detection. bacteria isolates were molecularly identified and susceptibility tests for antibiotic agents were ...201727889252
activity of ceftazidime-avibactam against multidrug-resistance enterobacteriaceae expressing combined mechanisms of resistance.antimicrobial resistance in enterobacteriaceae is increasing worldwide and is making treating infections caused by multidrug-resistant enterobacteriaceae a challenge. the use of β-lactam agents is compromised by microorganisms harboring extended-spectrum β-lactamases (esbls) and other mechanisms of resistance. avibactam is a non β-lactam agent that inhibits clinically relevant β-lactamases, such as esbl and ampc. the ceftazidime-avibactam combination (caz-avi) was recently approved for use in ce ...201627887765
prevalence of extended spectrum beta-lactamase producing klebsiella pneumoniae isolated from urinary tract infected patients.klebsiella pneumoniae, one of the bacterial agents associated with urinary tract infection has been often implicated as a major extended spectrum beta-lactamase (esbl) producer in last few decades. this study was designed to assess the prevalence of esbl producing klebsiella pneumoniae in urinary isolates at a tertiary care hospital in kathmandu, nepal, from july to december 2014.201627885293
phenotypic and genomic characterization of the antimicrobial producer rheinheimera sp. eprs3 isolated from the medicinal plant echinacea purpurea: insights into its biotechnological relevance.in recent years, there has been increasing interest in plant microbiota; however, despite medicinal plant relevance, very little is known about their highly complex endophytic communities. in this work, we report on the genomic and phenotypic characterization of the antimicrobial compound producer rheinheimera sp. eprs3, a bacterial strain isolated from the rhizospheric soil of the medicinal plant echinacea purpurea. in particular, eprs3 is able to inhibit growth of different bacterial pathogens ...201727884784
coexistence of fosfomycin and colistin resistance in klebsiella pneumoniae: whole-genome shotgun sequencing.resistance to colistin is a major threat that limits therapeutic choices for treating carbapenem-resistant klebsiella pneumoniae infections. herein, we report the draft genome sequences of two colistin-resistant k. pneumoniae isolates (ba41763 and b6753). the sequence data indicate that ba41763 and b6753 contain genomes of ~5.9 and 5.7 mb in size with several plasmids.201627881539
antibacterial and antifungal potentials of the solvents extracts from eryngium caeruleum, notholirion thomsonianum and allium consanguineum.herbal medicines have long been used for various ailments in various societies and natural bioactive compounds are gaining more and more importance due to various factors. in this context, three plant species i.e., eryngium caeruleum, notholirion thomsonianum and allium consanguineum have been aimed for the scientific verification of their purported traditional uses against various infectious diseases.201627881119
ereply. diagnostic intricacies and fortuitous treatment approaches for carbapenem-resistant klebsiella pneumoniae. 201627880708
ecomment. diagnostic intricacies and fortuitous treatment approaches for carbapenem-resistant klebsiella pneumoniae. 201627880707
successful elimination of extended-spectrum beta-lactamase (esbl)-producing nosocomial bacteria at a neonatal intensive care unit.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are highly dangerous to neonates. at our neonatal intensive care unit (nicu), the presence of these bacteria became so threatening in 2011 that immediate intervention was required.201627878781
piperacillin/tazobactam as an alternative antibiotic therapy to carbapenems in the treatment of urinary tract infections due to extended-spectrum β-lactamase-producing enterobacteriaceae: an in silico pharmacokinetic study.piperacillin/tazobactam (tzp) as an alternative treatment to carbapenems for infections involving extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-pe) remains debated. in this study, the probabilities of pharmacodynamic (pd) target attainment with different tzp regimens in esbl-producing escherichia coli (esbl-ec) and klebsiella pneumoniae (esbl-kp) were evaluated in the context of pyelonephritis. minimum inhibitory concentrations (mics) of 144 esbl-ec and 111 esbl-kp from ...201727876276
[multiple organ failure by serotype k1 klebsiella pneumoniae]. 201627876187
doping polypyrrole films with 4-n-pentylphenylboronic acid to enhance affinity towards bacteria and dopamine.here we demonstrate the use of a functional dopant as a fast and simple way to tune the chemical affinity and selectivity of polypyrrole films. more specifically, a boronic-functionalised dopant, 4-n-pentylphenylboronic acid (pba), was used to provide to polypyrrole films with enhanced affinity towards diols. in order to prove the proposed concept, two model systems were explored: (i) the capture and the electrochemical detection of dopamine and (ii) the adhesion of bacteria onto surfaces. the c ...201627875555
cefiderocol, a siderophore cephalosporin for gram-negative bacterial infections: pharmacokinetics and safety in subjects with renal impairment.cefiderocol, a new injectable siderophore cephalosporin antibiotic, has promising in vitro and in vivo activity against gram-negative bacteria including multidrug-resistant pseudomonas aeruginosa, acinetobacter baumannii, and klebsiella pneumoniae. cefiderocol is mainly renally eliminated. the pharmacokinetics and safety of cefiderocol in subjects with renal impairment were assessed following a single 1000-mg intravenous 1-hour infusion of cefiderocol. subjects with mild, moderate, or severe ren ...201627874971
a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute.in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ...201627874136
in vitro activity of ceftaroline tested against isolates from the asia-pacific region and south africa (2011).during 2011, 29 medical centres in eight asia-pacific countries and south africa submitted a total of 3697 bacterial pathogens for surveillance testing, of which 39.1% were from respiratory tract, 27.9% from skin and skin-structure, 19.2% from bloodstream, 5.1% from urinary tract and 8.7% from miscellaneous infection types. meticillin-resistant staphylococcus aureus (mrsa) constituted 38.9% of the 1114 s. aureus. the mrsa rate was 53.5% for respiratory tract infections and 31.8% for skin and ski ...201427873727
in vitro activity of tigecycline and comparators against carbapenem-resistant enterobacteriaceae in africa-middle east countries: test 2007-2012.multidrug-resistant (mdr) enterobacteriaceae are an emerging concern for healthcare providers. infections caused by mdr pathogens are associated with increased costs, length of hospital stay, and morbidity and mortality rates. carbapenem-resistant enterobacteriaceae (cre) continue to increase, and infections with these organisms are observed worldwide not only as hospital-acquired infections but also as community-acquired infections. increasing antimicrobial resistance dictates the need for cont ...201427873726
spectrum and patterns of antimicrobial resistance of uropathogens isolated from a sample of hospitalised lebanese patients with urinary tract infections.a retrospective study was carried out to assess the current distribution and antimicrobial susceptibility patterns of uropathogens isolated from patients with community-acquired (ca) or hospital-acquired (ha) urinary tract infections (utis) who were admitted to the largest governmental hospital in beirut city (lebanon) in 2009-2012. overall, 292 uropathogens were isolated. enterobacteriaceae constituted 81% of the isolated uropathogens, with escherichia coli, klebsiella pneumoniae and proteus mi ...201427873725
antimicrobial susceptibility among gram-positive and gram-negative isolates collected in europe between 2004 and 2010.here we report on the in vitro activity of a suite of antimicrobial agents against gram-negative and gram-positive pathogens collected in europe between 2004 and 2010 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.). clinical and laboratory standards institute (clsi) broth microdilution methodologies were used to determine minimum inhibitory concentrations. clsi interpretive criteria were applied for all antimicrobial agents to establish susceptibility; european committee ...201427873722
high burden of antimicrobial drug resistance in asia.the rapid development of antimicrobial resistance among micro-organisms is a serious public health concern. moreover, the dissemination of antibiotic-resistant bacteria makes this issue a global problem, and asia is no exception. for example, since new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae were identified in india, further spread of ndm has become a worldwide threat. however, the epidemiology of antibiotic-resistant bacteria in asia may be different to other regions, and c ...201427873720
investigation of a possible outbreak of ndm-5-producing st16 klebsiella pneumoniae among patients in denmark with no history of recent travel using whole-genome sequencing. 201527873714
efficacy of tigecycline-colistin combination in the treatment of carbapenem-resistant klebsiella pneumoniae endocarditis.here we discuss the efficacy of colistin-tigecycline combination in the treatment of multidrug-resistant (mdr) klebsiella pneumoniae infective endocarditis (ie). we report a case of a 67-year-old head-injured patient who developed a carbapenem-resistant k. pneumoniae ie. the patient was treated with colistin-tigecycline combination, with a favourable outcome. in conclusion, colistin-tigecycline combination may be a possible combination in the therapy of ie caused by mdr enterobacteriaceae.201527873712
in vivo horizontal dissemination of the blakpc-2 gene carried on diverse genetic platforms among clinical isolates of enterobacteriaceae.this study investigated the molecular characteristics of six blakpc-positive enterobacteriaceae recovered from three patients in argentina. antimicrobial susceptibility testing was performed following clinical and laboratory standards institute (clsi) 2014 recommendations. molecular characterisation of the isolates was performed by biparental conjugation, pcr, sequencing, s1 nuclease restriction, and southern blot hybridisation with a blakpc probe using standard protocols and conditions. the iso ...201527873711
antimicrobial resistance and resistance mechanisms of enterobacteriaceae in icu and non-icu wards in europe and north america: smart 2011-2013.intensive care units (icus) are often described as hotbeds of antimicrobial resistance, with high rates of extended-spectrum β-lactamase (esbl)-producing and multidrug-resistant (mdr) enterobacteriaceae. data from the smart study were used to examine differences between the susceptibility of enterobacteriaceae from icu and non-icu wards in europe and north america. in total, 21,470 enterobacteriaceae isolates from intra-abdominal and urinary tract infections were collected at 90 sites in 20 euro ...201527873708
first cases of kpc-type carbapenemase-producing bacteria in patients in new zealand hospitals.the emergence and global spread of klebsiella pneumoniae carbapenemases (kpcs) is a significant public health problem. between october 2010 and july 2013, kpc-producing k. pneumoniae were isolated from four patients in new zealand hospitals. these cases are the first known isolations of kpc-producing organisms in new zealand. all four patients were transferred from, or had recently been in, hospitals in countries where kpc-producing organisms are prevalent (china, india, greece and italy). the b ...201427873697
emergence of plasmid mediated carbapenemase oxa-48 in a klebsiella pneumoniae strain in algeria.a carbapenem resistant klebsiella pneumoniae strain was isolated in october 2011 in the pediatric unit of the hôpital central de l'armée in algeria, this strain have been confirmed for the production of oxa-48 carbapenemase. the blaoxa-48 gene was located on a self conjugative plasmid of 70kb. multilocus sequence typing indicated the presence of the sequence type st-307. this is the first isolation of oxa-48-producing k. pneumoniae in algeria.201427873696
investigation of tigecycline bactericidal activity: optimisation of laboratory testing.the objectives of this study were to optimise the conditions for bactericidal testing of tigecycline and to investigate its bactericidal activity against clinical isolates of gram-positive and gram-negative bacteria. tigecycline is the first in a new class of glycylcycline antibiotics exhibiting in vitro activity against a broad range of bacteria, including multidrug-resistant organisms. its bactericidal activity in vitro has not been extensively investigated using multiple test conditions. five ...201427873686
intrapersonal mutation of rmpa and rmpa2: a reason for negative hypermucoviscosity phenotype and low virulence of rmpa-positive klebsiella pneumoniae isolates.two klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (pfge). however, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. a previously unrelated liver abscess k. pneumoniae hypermucoviscous isolate was used as a control. pcr, dna cloning and sequencing for the plasmid-borne rmpa and rmpa2 genes and the chromo ...201527873663
carbapenem-resistant klebsiella pneumoniae infections in a greek intensive care unit: molecular characterisation and treatment challenges.acquisition of carbapenemase-producing klebsiella pneumoniae (cp-kp) strains poses a major threat to critically ill patients. the objectives of this study were to describe the epidemiology of cp-kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mortality of intensive care unit (icu) patients in a greek hospital. a prospective, observational study was conducted in a nine-bed general icu over a 2-year period (april 2010-march ...201527873660
characterisation of extended-spectrum β-lactamases among klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in mozambique.the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in manhiça, mozambique. esbls were investigated among ceftriaxone-non-susceptible k. pneumoniae clinical isolates recovered between 2004 and 2009. characterisation of blactx-m, blashv, blaoxa and blatem genes was performed by pcr and sequencing. epidemiological relationships were established by phy ...201527873645
carbapenem-hydrolysing β-lactamase kpc-2 in klebsiella pneumoniae isolated in ecuadorian hospitals. 201327873619
emergence of blakpc-3-tn4401a in klebsiella pneumoniae st512 in the municipal wastewater treatment plant and in the university hospital of a town in central italy.in this study, 20 carbapenem-resistant environmental klebsiella pneumoniae strains were found to correlate with 18 clinical k. pneumoniae isolates from the teaching hospital of l'aquila city, italy. all strains analysed by multilocus sequence typing (mlst) were included in the same clone (st512), and pulsed-field gel electrophoresis demonstrated a genetic relationship between the clinical isolates and most environmental strains. both environmental and clinical strains harboured the same mobile g ...201327873616
ndm-1- and oxa-163-producing klebsiella pneumoniae isolates in cairo, egypt, 2012.here we describe carbapenem resistance determinants in two klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in cairo, egypt. pcr and sequencing were used to detect and characterise β-lactamase genes. clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst). the first k. pneumoniae isolate carried the blandm-1 gene and the second isolate carri ...201327873615
polymicrobial bloodstream infections: epidemiology and impact on mortality.the aim of this study was to investigate the impact of polymicrobial bloodstream infections (pbsis) on the outcome of sepsis in an area where antimicrobial resistance is of concern. this was a retrospective analysis of data collected prospectively from patients developing bsi outside of an intensive care unit (non-icu patients) or after icu admission. demographics and clinical characteristics were compared for patients with pbsi versus monomicrobial bsi (mbsi) and following stratification by icu ...201327873614
in vivo transmission of a plasmid containing the kpc-2 gene in a single patient.here we describe a case of in vivo horizontal interspecies transmission of a kpc-2-producing plasmid from a klebsiella pneumoniae to an enterobacter aerogenes strain in the same patient. the patient's gut flora initially contained a carbapenem-susceptible e. aerogenes strain and 10 days after admission a kpc-2-positive k. pneumoniae. three months after admission, a kpc-2-positive e. aerogenes was identified in fecal surveillance cultures. this isolate was isogenic with the initial e. aerogenes a ...201327873604
surveillance trends of carbapenem-resistant enterobacteriaceae from singapore, 2010-2013.trends in carbapenem-resistant enterobacteriaceae (cre) collected from hospitals nationwide in singapore over 3 years are presented. hospital isolates with imipenem or meropenem minimum inhibitory concentrations (mics) of >1mg/l were sent to the national public health laboratory for further investigation. a total of 400 cre were submitted, 227 (56.8%) of which carried a carbapenemase gene. blandm was the most common (130/400; 32.5%), followed by blaoxa-48-like (blaoxa-48, -181, -232) (55/400; 13 ...201427873598
global antibacterial resistance: the never-ending story.bacterial resistance is undoubtedly recognised as a major medical challenge in most healthcare systems. resistance-determining genes, mostly in combination, and multidrug-resistant (mdr) pathogens are spreading with unprecedented speed. well known resistance carriers with high clinical impact include the gram-positive organisms staphylococcus aureus and enterococcus spp. in contrast to these organisms that are usually still treatable with newer alternative antibacterial drugs, some gram-negative ...201327873580
antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital of punjab, india.we determined the prevalence and antibiotic susceptibilities patterns of bacterial isolates from pus samples collected from patients in a tertiary care hospital of punjab, india. e. coli was the most prevalent pathogen (51.2%) followed by staphylococcus aureus (21%), klebsiella pneumoniae (11.6%), pseudomonas aeruginosa (5.8%), citrobacter spp. (3.5%), acinetobacter baumannii (2.3%), proteus mirabilis (2.3%), and streptococcus spp. (2.3%). e. coli, k. pneumoniae, a. baumannii, and citrobacter is ...201627872643
correction for leangapichart et al., acquisition of mcr-1 plasmid-mediated colistin resistance in escherichia coli and klebsiella pneumoniae during hajj 2013 and 2014. 201627872110
polymyxin b in combination with rifampin and meropenem against polymyxin b-resistant kpc-producing klebsiella pneumoniae.safe and effective therapies are urgently needed to treat polymyxin-resistant kpc-producing klebsiella pneumoniae infections and suppress the emergence of resistance. we investigated the pharmacodynamics of polymyxin b, rifampin, and meropenem alone and as polymyxin b-based double and triple combinations against kpc-producing k. pneumoniae isolates. the rates and extents of killing with polymyxin b (1 to 128 mg/liter), rifampin (2 to 16 mg/liter), and meropenem (10 to 120 mg/liter) were evaluate ...201727872078
occurrence of diverse antimicrobial resistance determinants in genetically unrelated biocide tolerant klebsiella pneumoniae.nosocomial infections due to klebsiella pneumoniae is a significant problem in health care settings worldwide. in this study, we examined the antimicrobial susceptibility, genetic profiles and mechanisms of antibiotic resistance in k. pneumoniae isolates of indian origin. to our knowledge this is the first report demonstrating the high prevalence of β-lactamases, aminoglycoside modifying enzymes, quinolone resistance genes besides demonstrating the involvement of active efflux in k. pneumoniae i ...201627870879
occurrence of carbapenemase-producing klebsiella pneumoniae and escherichia coli in the european survey of carbapenemase-producing enterobacteriaceae (euscape): a prospective, multinational study.gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in europe make it difficult to contain carbapenemase-producing enterobacteriaceae. we report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing klebsiella pneumoniae and escherichia coli in european hospitals.201727866944
[molecular epidemiology of carbapenemase-producing enterobacteriaceae infection/colonisation in a hospital in madrid].a description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital.201627866753
synthesis of silver nanoparticles by endosymbiont pseudomonas fluorescens ca 417 and their bactericidal activity.the present study emphasizes on biogenic synthesis of silver nanoparticles and their bactericidal activity against human and phytopathogens. nanoparticle synthesis was performed using endosymbiont pseudomonas fluorescens ca 417 inhabiting coffea arabica l. synthesized nanoparticles were characterized using hyphenated spectroscopic techniques such as uv-vis spectroscopy which revealed maximum absorption 425nm. fourier transform infrared spectroscopy (ftir) analysis revealed the possible functiona ...201627866607
disposable bronchoscope model for simulating endoscopic reprocessing and surveillance cultures.background endoscope-associated infections are reported despite following proper reprocessing methods. microbiological testing can confirm the adequacy of endoscope reprocessing. multiple controversies related to the method and interpretation of microbiological testing cultures have arisen that make their routine performance a complex target. objective we conducted a pilot study using disposable bronchoscopes (dbs) to simulate different reprocessing times and soaking times and to compare high-le ...201727866488
epidemiology of infections caused by polymyxin-resistant pathogens.confronting the storm of carbapenemase-producing gram-negative pathogens and thus facing the threat of untreatable infections, the medical community revived colistin. not long since its re-introduction and despite the fact that resistance to colistin at least in escherichia coli is rare, chromosomally-mediated colistin resistance in metallo-β-lactamase-producing klebsiella pneumoniae strains was reported in 2004 from greece. subsequent studies revealed the highest predominance in italy (38%) and ...201627865627
synergistic combinations of polymyxins.the proliferation of extensively drug-resistant gram-negative pathogens has necessitated the therapeutic use of colistin and polymyxin b. however, treatment failures with polymyxin monotherapies and the emergence of polymyxin resistance have catalysed the search for polymyxin combinations that synergistically kill polymyxin-susceptible and -resistant organisms. this mini-review examines recent (2011-2016) in vitro and in vivo studies that have attempted to identify synergistic polymyxin combinat ...201627865626
activity of norspermidine on bacterial biofilms of multidrug-resistant clinical isolates associated with persistent extremity wound infections.biofilm formation is a major virulence factor for numerous pathogenic bacteria and is cited as a central event in the pathogenesis of chronic human infections, which is in large part due to excessive extracellular matrix secretion and metabolic changes that occur within the biofilm rendering them highly tolerant to antimicrobial treatments. polyamines, including norspermidine, play central roles in bacterial biofilm development, but have also recently been shown to inhibit biofilm formation in s ...201627864804
c-terminal modification and multimerization increase the efficacy of a proline-rich antimicrobial peptide.two series of branched tetramers of the proline-rich antimicrobial peptide (pramp), chex1-arg20, were prepared to improve antibacterial selectivity and potency against a panel of gram-negative nosocomial pathogens including escherichia coli, klebsiella pneumoniae, acinetobacter baumannii and pseudomonas aeruginosa. first, tetramerization was achieved by dithiomaleimide (dtm) conjugation of two c-terminal-cysteine bearing dimers that also incorporated c-terminal peptide chemical modification. dtm ...201727862429
how often do clinically diagnosed catheter-associated urinary tract infections in nursing homes meet standardized criteria?to determine the relationship between clinically diagnosed catheter-associated urinary tract infection (cauti) and standardized criteria and to assess microorganism-level differences in symptom burden in a cohort of catheterized nursing home (nh) residents.201727858954
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