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risk factors of mortality in bloodstream infections caused by klebsiella pneumonia: a single-center retrospective study in china.the prevalence of klebsiella pneumonia bloodstream infections (kp-bsis) is increasing worldwide. few study reports focus on the kp-bsis published in mainland china over the previous years. this study aimed to describe the risk factors of mortality from kp-bsis.a retrospective study was conducted in a teaching hospital in shanghai, china, for a period of 4 years. risk factors related to the patient mortality were analyzed using the binary logistic regression model.of 104 patients with kp-bsis, th ...201728858116
impact of a mixed educational and semi-restrictive antimicrobial stewardship project in a large teaching hospital in northern italy.the overuse of antimicrobials favors the dissemination of antimicrobial resistance, as well as invasive fungal diseases and clostridium difficile infections (cdi). in this study, we assessed the impact of a mixed educational and semi-restrictive antimicrobial stewardship (ams) project in a large teaching hospital in italy.201728856589
genomic epidemiology of ndm-1-encoding plasmids in latin american clinical isolates reveals insights into the evolution of multidrug resistance.bacteria that produce the broad-spectrum carbapenem antibiotic new delhi metallo-β-lactamase (ndm) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistance mechanism. although it is believed that the associated resistance gene blandm-1 originated in acinetobacter spp., the role of enterobacteriaceae in its dissemination remains unclear. in this study, we used whole genome sequenci ...201728854628
low prevalence of carbapenem-resistant enterobacteriaceae among wounded military personnel.multidrug-resistant organisms (mdros) are a global health problem that affect both civilian and military populations. among wounded warriors, mdros further complicate the care of trauma-related infections, resulting in extended duration of hospitalization, as well as increased morbidity and mortality. during the wars in iraq and afghanistan, extended spectrum β-lactamase-producing enterobacteriaceae were frequently isolated from wounded warriors. the potential emergence of difficult-to-treat car ...201728853114
in vitro antimicrobial activity screening of xylaria hypoxylon.fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey.201628852719
isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures.dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran.201728852636
honey-based hydrogel: in vitro and comparative in vivo evaluation for burn wound healing.honey was used to treat wounds since ancient times till nowadays. the present study aimed at preparing a honey-based hydrogel and assay its antimicrobial properties and wound healing activity; in-vitro and in-vivo. topical honey hydrogel formulations were prepared using three honey concentrations with gelling agents; chitosan and carbopol 934. the prepared formulae were evaluated for ph, spreadability, swelling index, in-vitro release and antimicrobial activity. the ph and spreadability were in ...201728851905
pharmacokinetics and pharmacodynamics of temocillin.temocillin, a 6-α-methoxy derivative of ticarcillin, is a forgotten antibiotic that has recently been rediscovered, and issues about clinical breakpoints and optimal therapeutic regimens are still ongoing. temocillin spectrum is almost restricted to enterobacteriaceae. the addition of the α-methoxy moiety on ticarcillin confers resistance to hydrolysis by ambler classes a and c β-lactamases (extended spectrum β-lactamases, klebsiella pneumoniae carbapenemase and ampc hyperproduced enzymes). temo ...201728849402
validation of 2-(18)f-fluorodeoxysorbitol ((18)f-fds) as a potential radiopharmaceutical for imaging bacterial infection in the lung.2-(18)f-fluorodeoxysorbitol ((18)f-fds) has been shown to be a promising agent with high selectivity and sensitivity in imaging bacterial infection. the objective of our study was to validate (18)f-fds as a potential radiopharmaceutical for imaging bacterial infection longitudinally in the lung. methods: albino c57 female mice were intratracheally inoculated with either live or dead klebsiella pneumoniae (kp) to induce either lung infection or inflammation. one group of mice was imaged to monito ...201728848037
vaborbactam: spectrum of beta-lactamase inhibition and impact of resistance mechanisms on activity in enterobacteriaceae.vaborbactam (formerly rpx7009) is a new beta-lactamase inhibitor based on a cyclic boronic acid pharmacophore. the spectrum of beta-lactamase inhibition by vaborbactam and the impact of bacterial efflux and permeability on its activity were determined using a panel of strains with cloned beta-lactamases from various classes and a panel of klebsiella pneumoniae carbapenemase (kpc)-3 producing isogenic strains with various combinations of efflux and porin mutations. vaborbactam is a potent inhibit ...201728848018
[formulation of neomycin and streptomycin for the digestive decontamination of carbapenemases producing klebsiella pneumoniae]. 201728847258
klebsazolicin inhibits 70s ribosome by obstructing the peptide exit tunnel.whereas screening of the small-molecule metabolites produced by most cultivatable microorganisms often results in the rediscovery of known compounds, genome-mining programs allow researchers to harness much greater chemical diversity, and result in the discovery of new molecular scaffolds. here we report the genome-guided identification of a new antibiotic, klebsazolicin (klb), from klebsiella pneumoniae that inhibits the growth of sensitive cells by targeting ribosomes. a ribosomally synthesize ...201728846667
prevalence of β-lactamase genes in domestic washing machines and dishwashers and the impact of laundering processes on antibiotic resistant bacteria.to investigate the prevalence of β-lactamase genes in domestic washing machines and dishwashers and the decontamination efficacy of laundering.201728845592
evaluation of a rapid immunochromatographic test for detection of distinct variants of klebsiella pneumoniae carbapenemase (kpc) in enterobacteriaceae.the rapid detection of kpc-producing enterobacteriaceae by microbiology laboratories has been required for infectious control programs. herein we evaluated the performance of a novel immunochromatographic test for detecting kpc-2-, kpc-3-, kpc-4-, kpc-6-, kpc-7-, kpc-8-, and kpc-11-producing isolates and the influence of different growth media on the test performance.201728844719
photodynamic inactivation of multiresistant bacteria (kpc) using zinc(ii)phthalocyanines.the worldwide increase in antibiotic resistance has led to search of alternatives anti-microbial therapies such as photodynamic inactivation. the aim of this paper was to evaluate the photodynamic activity in vitro of a neutral and two cationic zn phthalocyanines. their photokilling activity was tested on escherichia coli atcc 25922 and klebsiella pneumoniae carbapenemase (kpc)-producing. after treating bacteria with phthalocyanines, the cultures were irradiated with white light. as a result, th ...201728844390
risk factors and molecular epidemiology of extended-spectrum β-lactamase-producing klebsiella pneumoniae in xiamen, china.the aim of this study was to evaluate the risk factors for pneumonia due to extended-spectrum β-lactamase-producing klebsiella pneumoniae (esbl-kp) and to analyse the molecular epidemiology of esbl-kp in xiamen, china.201728842361
effect of combination therapy containing a high dose carbapenem on mortality in patients with carbapenem-resistant klebsiella pneumoniae bloodstream infection.to evaluate the impact of high-dose (hd) carbapenem-based combination therapy on clinical outcome in patients with monomicrobial carbapenem-resistant klebsiella pneumoniae (cr-kp) bloodstream-infection (bsi).201728842283
a shorter period of therapy is associated with higher mortality in bloodstream infections caused by carbapenemase-producing klebsiella pneumoniae in a brazilian centre. 201728841082
comparison of a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform, asta microidsys, with bruker biotyper for species identification.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, with its accuracy and speed, is widely used for bacterial identification. the asta microidsys system (asta, korea) was recently developed for species identification. we compared its performance with that of bruker biotyper (bruker daltonics, germany). microbes were recovered from sputum, urine, and pus samples from patients admitted to a tertiary care hospital in korea from january to april 2016. matrix solution (α-cya ...201728840993
membrane-active macromolecules kill antibiotic-tolerant bacteria and potentiate antibiotics towards gram-negative bacteria.chronic bacterial biofilms place a massive burden on healthcare due to the presence of antibiotic-tolerant dormant bacteria. some of the conventional antibiotics such as erythromycin, vancomycin, linezolid, rifampicin etc. are inherently ineffective against gram-negative bacteria, particularly in their biofilms. here, we report membrane-active macromolecules that kill slow dividing stationary-phase and antibiotic tolerant cells of gram-negative bacteria. more importantly, these molecules potenti ...201728837596
clinical characteristics of bloodstream infections in pediatric acute leukemia: a single-center experience with 231 patients.acute leukemia is the most common pediatric hematological malignancy. bloodstream infections (bsis) are severe complications in these patients during chemotherapy. this study aimed to explore the clinical presentation and etiology of bsi, as well as the common sites of infection, and to provide a basis for the rational regarding antibiotic use.201728836551
trends in antimicrobial resistance against enterobacteriaceae strains isolated from blood: a 10-year epidemiological study in mainland china (2004-2014).antimicrobial resistance is a serious problem that compromises the empirical treatment of infections, resulting in a lack of effective antibiotics and high medical expenses. here, we aimed to monitor the trends in antimicrobial resistance among enterobacteriaceae isolated from blood samples in mainland china.201728836547
cofactor engineering for enhanced production of diols by klebsiella pneumoniae from co-substrate.diols, such as 1,3-propanediol (1,3-pdo) and 2,3-butanediol (2,3-bdo), have several promising properties for many synthetic reactions. here, the cofactor engineering strategy, including the construction of entner-doudoroff pathway and transhydrogenase-based nadh regeneration system, was applied in producing diols from mixtures of glucose and glycerol. entner-doudoroff pathway had a high regeneration rate of nad(p)h. this work described a strategy to administrate intracellular nadh/nad(+) ratio a ...201728834346
chemical and antimicrobial analyses of sideritis romana l. subsp. purpurea (tal. ex benth.) heywood, an endemic of the western balkan.a comprehensive study on essential oil and different solvent extracts of sideritis romana l. subsp. purpurea (tal. ex benth.) heywood (lamiaceae) from montenegro is reported. the gas chromatography-mass spectrometry analysis of the essential oil revealed a total of 43 components with bicyclogermacrene (23.8%), germacrene d (8%), (e)-caryophyllene (7.9%) and spathulenol (5.5%) as the major ones. sesquiterpenoid group was found to be the most dominant one (64.8%), with 19.9% of the oxygenated form ...201728832536
a case of emphysematous cystitis caused by klebsiella pneumoniae.emphysematous cystitis is a rare type of urinary tract infection that is characterized by air pockets within the bladder wall and lumen, which come from gas that is mainly produced by gram-negative bacteria, notably escherichia coli. this infection is more common in older women with poorly controlled diabetes. an abdominal computerized tomography (ct) scan is the gold standard method to make the diagnosis. the infection can be life-threatening, so prompt treatment is essential. we present a case ...201728832314
klebsiella pneumoniae (st1519) producing kpc-19 carbapenemase in a patient undergoing selective digestive decontamination before liver transplant. 201728830804
parastomal gallbladder hernia in a septic patient.parastomal gallbladder herniation is a rare complication of enterostomies with only 6 previously reported cases. most cases have occurred in elderly women. patients typically presented with acute abdominal pain and the majority was managed operatively. here, we report the clinical course of an 88-year-old female who presented with signs of sepsis and minimal abdominal symptoms. she was subsequently found to have a parastomal gallbladder herniation and klebsiella pneumoniae bacteremia. given the ...201728828113
investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate.chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ...201728827963
measuring and modelling the response of klebsiella pneumoniae kpc prey to bdellovibrio bacteriovorus predation, in human serum and defined buffer.in worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. bdellovibrio bacteriovorus bacteria naturally prey on gram-negative pathogens, including antibiotic-resistant strains and so b. bacteriovorus have been proposed as "living antibiotics" to combat antimicrobially-resistant pathogens. predator-prey interactions are complex and can be altered by environmental components. to be effective b. bacteriovorus predation needs ...201728827526
antimicrobial activity of ceftazidime-avibactam tested against multidrug-resistant enterobacteriaceae and pseudomonas aeruginosa isolates from united states medical centers (2013--2016).the in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 enterobacteriaceae and 7,868 pseudomonas aeruginosa. isolates were consecutively collected from 94 us hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (jmi laboratories). enterobacteriaceae isolates resistant to carbapenems (cre) and/or ceftazidime-avibact ...201728827415
a simple phenotypic method for screening of mcr-1-mediated colistin resistance.to evaluate a novel method, named colistin-mac test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin mic reduction in the presence of dipicolinic acid (dpa).201728827120
construction of recombinant pdu metabolosome shells for small molecule production in corynebacterium glutamicum.bacterial microcompartments have significant potential in the area of industrial biotechnology for the production of small molecules, especially involving metabolic pathways with toxic or volatile intermediates. corynebacterium glutamicum is an established industrial workhorse for the production of amino acids and has been investigated for the production of a variety of further value-added products. herein, we describe components for the establishment of bacterial microcompartments as production ...201728826205
application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products.foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ...201728825446
antimicrobial activity of octenidine against multidrug-resistant gram-negative pathogens.multidrug-resistant (mr) gram-negative (gn) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. well-tolerated antiseptics, such as octenidine dihydrochloride (oct), may be a very useful tool in infection control to reduce the dissemination of mrgn. this study aimed to investigate the bactericidal activity of oct against international epidemic clones of mrgn. a set of five different species ...201728825186
ask2 bioactive compound inhibits mdr klebsiella pneumoniae by antibiofilm activity, modulating macrophage cytokines and opsonophagocytosis.the emergence and spread of pathogens harboring extended spectrum beta-lactamase (esbl) like carbapenem resistant gram negative bacteria are the major emerging threat to public health. of particular concern klebsiella pneumoniae carbapenamase- producing strains have been recorded worldwide. catheter associated urinary tract infections (cauti) caused by k. pneumoniae are significantly associated with morbidity and mortality. hence the present work was aimed to develop a strategy for addressing th ...201728824881
anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant klebsiella pneumoniae l17 in a bioelectrochemical system.3-hydroxypropionic acid (3-hp) is an important platform chemical which can be produced biologically from glycerol. klebsiella pneumoniae is an ideal biocatalyst for 3-hp because it can grow well on glycerol and naturally synthesize the essential coenzyme b12. on the other hand, if higher yields and titers of 3-hp are to be achieved, the sustained regeneration of nad(+) under anaerobic conditions, where coenzyme b12 is synthesized sustainably, is required.201728824709
stress-driven discovery of novel cryptic antibiotics from a marine fungus penicillium sp. bb1122.standard laboratory cultures have long been known to hinder activation of specific gene clusters which in turn hamper production of secondary metabolites with unique properties due to lack of innovation or the inability to trigger cryptic gene clusters' expression. due to challenges related to the avoidance of the isolation of replicated metabolites, resistance-developing pathogens are to be addressed by the scientific community worldwide in order to progress with novel and potent compounds whic ...201728824581
comparison of two whole-room uv-irradiation systems for enhanced disinfection of patient rooms contaminated with mrsa, carbapenemase-producing klebsiella pneumoniae and clostridium difficile spores.ultraviolet light decontamination systems are being used increasingly to supplement terminal disinfection of patient rooms. however efficacy may not be consistent in the presence of soil particularly against clostridium difficile spores.201728823547
incidence of cross infection in liver transplant patients. risk factors and role of nursinge.to determine the incidence and risk factors of infection in liver transplant recipients immediately post-transplant, during admission to a liver transplant unit during the immediate post-transplant admission.201728822713
pinocembrin, a novel histidine decarboxylase inhibitor with anti-allergic potential in in vitro.pinocembrin (5, 7- dihydroxy flavanone) is the most abundant chiral flavonoid found in propolis, exhibiting antioxidant, antimicrobial and anti-inflammatory properties. however, the effect of pinocembrin on allergic response is unexplored. thus, current study aimed at investigating the effects of pinocembrin on ige-mediated allergic response in vitro. a special emphasis was directed toward histidine decarboxylase (hdc) and other pro-allergic and pro-inflammatory mediators. preliminary studies, u ...201728821452
the effect of dietary bacterial organic selenium on growth performance, antioxidant capacity, and selenoproteins gene expression in broiler chickens.selenium (se) is an essential trace mineral in broilers, which has several important roles in biological processes. organic forms of se are more efficient than inorganic forms and can be produced biologically via se microbial reduction. hence, the possibility of using se-enriched bacteria as feed supplement may provide an interesting source of organic se, and benefit broiler antioxidant system and other biological processes. the objective of this study was to examine the impacts of inorganic se ...201728821244
ultrahigh antibacterial efficacy of meropenem-loaded chitosan nanoparticles in a septic animal model.antimicrobial resistance is one of the most significant health challenges worldwide. meropenem is a broad spectrum beta-lactam antibiotic that possesses high activity against gram-positive and gram-negative bacteria. however, it has a short plasma half-life, and thus requiring frequent administration of high doses. for the first time, meropenem-loaded chitosan nanoparticles were prepared and evaluated as a potential tool to overcome antimicrobial resistance and to improve pharmacokinetics of the ...201728821026
[to the state of the fetoplacental complex in women with the threat of abortion].the goal is a comprehensive study of the morphofunctional state of the mother-placenta-fetus system in placental insufficiency and infection. 250 pregnant and puerperas for the period 2008-2014 were examined. detection of bacterial flora was carried out in 35 pregnant women aged 20.4±0.8 years with the threat of abortion from an early gestation period and a prolonged persistent infection of the genitals, bacterial vaginosis. methods are applied: culture, ion chromatography, gas-liquid chromatogr ...201728820412
cost of nosocomial outbreak caused by ndm-1-containing klebsiella pneumoniae in the netherlands, october 2015-january 2016.during october-december 2015, 29 patients in a hospital in the netherlands acquired nosocomial infection with a multidrug-resistant, new delhi-metallo-β-lactamase-positive klebsiella pneumoniae strain. extensive infection control measures were needed to stop this outbreak. the estimated economic impact of the outbreak was $804,263; highest costs were associated with hospital bed closures.201728820386
prevalence study of plasmid-mediated ampc β-lactamases in enterobacteriaceae lacking inducible ampc from saudi hospitals.enterobacteriaceae encoding plasmid-mediated ampc (pampc) β-lactamases confer resistance to the third generation cephalosporins. pampc association with extended spectrum β-lactamases (esbls), plasmid-mediated quinolone resistance (pmqr) and aminoglycoside modifying enzymes (ames) is well documented. there are limited data regarding the epidemiology and clinical significance of pampc in saudi arabia. this study aimed to determine the prevalence of pampc and its coexistence with esbls, pmqr and am ...201728820112
draft genome sequence of klebsiella pneumoniae 704sk6, an oxa-48- and ctx-m-15-encoding wastewater isolate.the swiss wastewater isolate klebsiella pneumoniae 704sk6, encoding oxa-48 and ctx-m-15 β-lactamases, was fully sequenced. the assembly resulted in an open chromosome of 5,208,104 bp in size (g+c content, 57.6%) and four closed plasmid sequences of 209,651, 197,670, 65,998, and 63,605 bp in size.201728818913
characterization and formulation into solid dosage forms of a novel bacteriophage lytic against klebsiella oxytoca.to isolate and characterize bacteriophage lytic for the opportunistic pathogen klebsiella oxytoca and their formulation into a range of solid dosage forms for in-vitro testing.201728817689
emergence of two distinct subpopulations from klebsiella pneumoniae grown in the stimulated microgravity environment.to isolate and characterize the two phenotypically distinct subpopulations from klebsiella pneumoniae clonal cultures grown in the simulate microgravity environment.201728816520
multidrug-resistant enterobacteriaceae, pseudomonas aeruginosa, and vancomycin-resistant enterococci: three major threats to hematopoietic stem cell transplant recipients.hematopoietic stem cell transplant (hsct) recipients are uniquely threatened by the emergence of multidrug-resistant (mdr) bacteria because these patients rely on immediate active antimicrobial therapy to combat bacterial infections. this review describes the epidemiology and treatment considerations for three challenging mdr bacterial pathogens in hsct recipients: mdr enterobacteriaceae, including extended-spectrum β-lactamase (esbl)-producing and carbapenem-resistant enterobacteriaceae (cre), ...201728815897
a semi-synthetic glycoconjugate vaccine candidate for carbapenem-resistant klebsiella pneumoniae.hospital acquired infections are an increasingly serious health concern. infections caused by carpabenem-resistant klebsiella pneumoniae (cr-kp) are especially problematic, with a 50% average survival rate. cr-kp are isolated from patients with ever greater frequency: 7% within the eu but 62% in greece. at a time when antibiotics are becoming less effective, no vaccines to protect from this severe bacterial infection exist. here, we describe the convergent [3+3] synthesis of the hexasaccharide r ...201728815890
novel consortium of klebsiella variicola and lactobacillus species enhances the functional potential of fermented dairy products by increasing the availability of branched-chain amino acids and the amount of distinctive volatiles.identify novel bacterial taxa that could increase the availability of branched-chain amino acids and the amount of distinctive volatiles during skim milk fermentation.201728815819
add-on therapy with ertapenem in infections with multidrug resistant gram-negative bacteria: pediatric experience.optimal therapy for infections with carbapenem resistant gnb is not well established due to the weakness of data. patients presenting with bloodstream infections caused by multidrug resistant klebsiella pneumoniae were treated with a combination treatment. optimal therapy for infections with carbapenem resistant gram-negative bacteria is a serious problem in pediatric patients. we presented three cases who were successfully treated with addition of ertapenem to the combination treatment for bact ...201728815094
frequency of colistin and fosfomycin resistance in carbapenem-resistant enterobacteriaceae from a tertiary care hospital in karachi.management of infections with carbapenem-resistant enterobacteriaceae (cre) is challenging. in recent times, agents such as colistin and fosfomycin have been used in combination with other antibiotics to treat such infections. in this study, we aim to seek frequency of colistin and fosfomycin resistance in cre from pakistan.201728814888
effect of poly-hexamethylene biguanide hydrochloride (phmb) treated non-sterile medical gloves upon the transmission of streptococcus pyogenes, carbapenem-resistant e. coli, mrsa and klebsiella pneumoniae from contact surfaces.reduction of accidental contamination of the near-patient environment has potential to reduce acquisition of healthcare-associated infection(s). although medical gloves should be removed when soiled or touching the environment, compliance is variable. the use of antimicrobial-impregnated medical gloves could reduce the horizontal-transfer of bacterial contamination between surfaces.201728814284
epidemiology and outcomes of endophthalmitis in chronic dialysis patients: a 13-year experience in a tertiary referral center in taiwan.endophthalmitis is a severe eye infection leading to disabling outcome. because there were only a few case report illustrating endophthalmitis in chronic dialysis patient, we would like to investigate the epidemiology and clinical features of endophthalmitis in chronic dialysis patient in a tertiary referral center.201728814278
antibacterial properties of nonwoven wound dressings coated with manuka honey or methylglyoxal.manuka honey (mh) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. mh provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (mgo). aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both mh and mgo (at equivalent mgo concentrations) when applied as a physical coating to a nonwoven fabric wound dress ...201728813014
low prevalence of mcr-1-producing klebsiella pneumoniae in bloodstream infections in china. 201728811245
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
dipicolinic acid derivatives as inhibitors of new delhi metallo-β-lactamase-1.the efficacy of β-lactam antibiotics is threatened by the emergence and global spread of metallo-β-lactamase (mbl) mediated resistance, specifically new delhi metallo-β-lactamase-1 (ndm-1). by utilization of fragment-based drug discovery (fbdd), a new class of inhibitors for ndm-1 and two related β-lactamases, imp-1 and vim-2, was identified. on the basis of 2,6-dipicolinic acid (dpa), several libraries were synthesized for structure-activity relationship (sar) analysis. inhibitor 36 (ic50 = 80 ...201728809565
aggregation-induced-emission materials with different electric charges as an artificial tongue: design, construction, and assembly with various pathogenic bacteria for effective bacterial imaging and discrimination.imaging-based total bacterial count and type identification of bacteria play crucial roles in clinical diagnostics, public health, biological and medical science, and environmental protection. herein, we designed and synthesized a series of tetraphenylethenes (tpes) functionalized with one or two aldehyde, carboxylic acid, and quaternary ammonium groups, which were successfully used as fluorescent materials for rapid and efficient staining of eight kinds of representative bacterial species, incl ...201728809473
first case report of non-human primates (alouatta clamitans) with the hypervirulent klebsiella pneumoniae serotype k1 strain st 23: a possible emerging wildlife pathogen.hypervirulent strain of klebsiella pneumoniae genotype k1 isolates have recently emerged, causing severe pyogenic liver abscess complicated by devastating metastatic infections in humans.201728809435
rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of gram-negative clinical isolates.bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both gram-positive and gram-negative bacterial species requires at least 48-72 h. recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the micro ...201728808348
clinical outcome of dual colistin- and carbapenem-resistant klebsiella pneumoniae bloodstream infections: a single-center retrospective study of 75 cases in india.in this study, we retrospectively evaluated clinical outcomes of 75 patients with dual colistin- and carbapenem-resistant klebsiella pneumoniae bloodstream infections over a 5-year period in a single tertiary care hospital in india. we observed a high in-hospital mortality rate of 69.3%. our findings indicate the urgent need for new antibiotics to treat these infections.201728807425
incx3 plasmid harboring a non-tn4401 genetic element (ntekpc) in a hospital-associated clone of kpc-2-producing klebsiella pneumoniae st340/cg258.incx-type plasmids have achieved clinical significance for their contribution in the dissemination of genes confering resistance to carbapenems (most blakpc- and blandm-type genes) and polymyxins (mcr-type genes), both antibiotics considered last resort for multidrug-resistant gram-negative infections. in this study, we report the identification and complete sequence analysis of an incx3 plasmid (designated pkp1194a) carrying a non-tn4401 genetic element (ntekpc) of tnpr-tnpa (partial)-blakpc-2- ...201728807400
a five-year surveillance of carbapenemase-producing klebsiella pneumoniae in a pediatric hospital in china reveals increased predominance of ndm-1.to characterize carbapenem (cpm)-non-susceptible klebsiella pneumoniae (k. pneumoniae) and carbape-nemase produced by these strains isolated from beijing children's hospital based on a five-year surveillance.201728807096
simultaneous detection of 13 key bacterial respiratory pathogens by combination of multiplex pcr and capillary electrophoresis.lower respiratory tract infections continue to pose a significant threat to human health. it is important to accurately and rapidly detect respiratory bacteria. to compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (pcr) and capillary electrophoresis (mpce) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including streptococcus pneumoniae, haemophilus influ ...201728807095
high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria.we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea.201728805537
effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumoniae.objective to explore the effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumonia (kp).methods the broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of scutellaria baicalensis for tw518. vitek-32 system was used to assay tw518 susceptibility to antibiotics. kp biofilms were formed in vitro and stained with baclight live/dead stain. the class integron geneⅰ1 mrna expression was ...201628803607
isobutanol and 2-ketoisovalerate production by klebsiella pneumoniae via a native pathway.isobutanol is a valuable chemical and is considered a new generation biofuel. construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. here, we show that an isobutanol synthesis pathway exists naturally in klebsiella pneumoniae; however, this pathway is dormant in the wild-type bacterium. k. pneumoniae is a 2,3-butanediol producer, and the synthesis pathways of 2,3-butanediol, valine and isobutanol all start from condensation of two pyruvate molecules to ...201728802880
discovery of novel highly active and stable aspartate dehydrogenases.aspartate family amino acids (afaas) have important commercial values due to their wide spectrum of applications. most if not all afaas are produced under aerobic conditions which is energy-intensive. to establish a cost-effective anaerobic process for production of afaas, it holds great promise to develop a new pathway enabling the conversion of oxoloacetate into aspartate through direct amination which is catalyzed by aspartate dehydrogenase (aspdh). compared with the well studied aspartate am ...201728801651
occurrence of carbapenemase-producing enterobacteriaceae isolates in the wildlife: first report of oxa-48 in wild boars in algeria.the aim of the present study was to screen for the presence of carbapenemase-producing enterobacteriaceae (cpe) isolates from wild boars and barbary macaques in algeria. fecal samples were collected from wild boars (n = 168) and barbary macaques (n = 212), in bejaia, algeria, between september 2014 and april 2016. the isolates were identified and antimicrobial susceptibility was determined. carbapenem resistance determinants were studied using pcr and sequencing, while clonal relatedness was per ...201728799835
p1220-ctxm, a pkp048-related incfiik plasmid carrying bla ctx-m-14 and qnrb4.this study aimed to characterize plasmid-mediated antimicrobial resistance in clinical klebsiella pneumoniae 1220 carrying bla ctx-m-14 and qnrb4.201728799786
prostatic abscess after stapled hemorrhoidopexy caused by esbl extended spectrum beta lactamase producing klebsiella pneumoniae: an additional challenge to postoperative sepsis.postoperative septic complications of hemorrhoids surgical interventions are rare, but very serious with high mortality rate. early diagnosis and prompt therapy are essential to save patient's life. there are a good number of articles and case reports about these septic complications. we are presenting a case report of a prostatic abscess caused by extended spectrum beta lactamase (esbl) producing klebsiella pneumoniae after hemorrhoidopexy. our patient was a healthy middle aged saudi male who h ...201728798879
evolving beta-lactamase epidemiology in enterobacteriaceae from italian nationwide surveillance, october 2013: kpc-carbapenemase spreading among outpatients.extended-spectrum beta-lactamases (esbls), ampc-type beta-lactamases (acbls) and carbapenemases are among the most important resistance mechanisms in enterobacteriaceae. this study investigated the presence of these resistance mechanisms in consecutive non-replicate isolates of escherichia coli (n = 2,352), klebsiella pneumoniae (n = 697), and proteus mirabilis (n = 275) from an italian nationwide cross-sectional survey carried out in october 2013. overall, 15.3% of isolates were non-susceptible ...201728797330
prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients attending a secondary care hospital in zambia.antimicrobial resistance is an increasing global health problem. very little data on resistance patterns of pathogenic bacteria in low-income countries exist. the aim of this study was to measure the prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients in the resource constraint setting of a secondary care hospital in zambia. nasal and rectal samples from 50 in- and 50 outpatients were collected. patients were randomly selected and informed consent was obtained. nas ...201728797299
impact of carbapenem-resistant klebsiella pneumoniae (cr-kp) infections in kidney transplantation.carbapenem-resistant klebsiella pneumoniae (cr-kp) infections in solid organ transplant patients are progressively increasing and are associated with worse outcomes, although potential risk factors and therapeutic strategies are still not well defined.201728796391
successful containment of the first kpc-producing klebsiella pneumoniae outbreak in croatia.we report the first outbreak caused by klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae sequence type 258 (st 258) clone that occurred in croatia from may to december 2012.201728795847
the first enterobacter cloacae co-producing ndm and oxa-48 carbapenemases and interhospital spread of oxa-48 and ndm-producing klebsiella pneumoniae in turkey.the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae.201728792697
device-associated healthcare-associated infections (da-hai) and the caveat of multiresistance in a multidisciplinary intensive care unit.device-associated healthcare-associated infections (da-hai), including ventilator-associated pneumonia (vap), central-line-associated blood stream infection (clabsi), and catheter-related urinary tract infection (cauti), are considered as principal contributors to healthcare hazard and threat to patient safety as they can cause prolonged hospital stay, sepsis, and mortality in the icu. the study intends to characterize da-hai in a tertiary care multidisciplinary icu of a teaching hospital in eas ...201728790779
structural and functional insights into the lipopolysaccharide abc transporter lptb2fg.the cell surface of most gram-negative bacteria contains lipopolysaccharide that is essential for their viability and drug resistance. a 134-kda protein complex lptb2fg is unique among atp-binding cassette transporters because it extracts lipopolysaccharide from the external leaflet of the inner membrane and propels it along a filament that extends across the periplasm to directly deliver lipopolysaccharide into the external leaflet of the outer membrane. here we report the crystal structure of ...201728790314
molecular epidemiology of klebsiella pneumoniae invasive infections over a decade at kilifi county hospital in kenya.multidrug resistant (mdr) klebsiella pneumoniae is a common cause of nosocomial infections worldwide. recent years have seen an explosion of resistance to extended-spectrum β-lactamases (esbls) and emergence of carbapenem resistance. here, we examine 198 invasive k. pneumoniae isolates collected from over a decade in kilifi county hospital (kch) in kenya. we observe a significant increase in mdr k. pneumoniae isolates, particularly to third generation cephalosporins conferred by esbls. using who ...201728789913
diversity, virulence, and antimicrobial resistance of the kpc-producing klebsiella pneumoniae st307 clone.the global spread of klebsiella pneumoniae producing klebsiella pneumoniae carbapenemase (kpc) has been mainly associated with the dissemination of high-risk clones. in the last decade, hospital outbreaks involving kpc-producing k. pneumoniae have been predominantly attributed to isolates belonging to clonal group (cg) 258. however, results of recent epidemiological analysis indicate that kpc-producing sequence type (st) 307, is emerging in different parts of the world and is a candidate to beco ...201728785421
geographical variation in therapy for bloodstream infections due to multidrug-resistant enterobacteriaceae: a post hoc analysis of the increment study.we aimed to describe regional differences in therapy for bloodstream infection (bsi) caused by extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) or carbapenemase-producing enterobacteriaceae (cpe). 1,482 patients in 12 countries were included from an observational study of bsi caused by esbl-e or cpe. multivariate logistic regression was used to calculate adjusted odds ratios (aors) for the influence of country of recruitment on empirical use of β-lactam/β-lactamase inhibitors ...201728782704
β-lacta(®) test performance for detection of extended-spectrum-β-lactamase-producing gram negative bacilli directly on bronchial aspirates samples: a validation study.incidence of extended spectrum β-lactamase-producing gram negative bacilli (esbl-p-gnb)-related infections is worryingly increasing worldwide. esbl-p-gnb detection directly on bronchial aspirate samples (bas) performed for suspected pneumonia may help save empirical carbapenems. our objectives were to optimize β-lacta(®) test (blt) realization and evaluate blt performance for esbl-p-gnb detection directly on bas.201728782646
emergence of low virulent carbapenem-resistant hypermucoviscous klebsiella pneumoniae in china. 201728782563
the occurrence of ctx-m-25-producing enterobacteriaceae in day-old broiler chicks in japan.day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between january and july 2016. sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant enterobacteriaceae. cefotaxime (ctx)-resistant klebsiella pneumoniae (1 isolate) and enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. conjugation experiments revealed that th ...201728781343
antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers.there has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. however, resistance of bacteria from hospital environments has been less frequently investigated.201728780198
photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles.multidrug resistant (mdr) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. nanoparticle-based photodynamic therapy (pdt) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with pdt is quite unspecific. herein, we show concanavalin-a (cona) directed dextran capped gold nanoparticles (gnpdex-cona) enhanced the efficacy and selectivity of methylene blue (mb) induced killing of ...201728778019
analysis of site-directed mutations in the α-and β-subunits of klebsiella pneumoniae nitrogenase.using directed mutagenesis, amino acid substitutions have been made in the α- and β-subunits of the klebsiella pneumoniae nitrogenase component 1 at positions normally occupied by conserves cysteine or tyrosine residues. nif', nif and intermediate pheno-types have been obtained. to extend our earlier biochemical characterization (kent et al, 1989) the electrophoretic mobiliy of component 1 of the mutant and wild-type nitrogenases has been analysed by non-denaturing gel electrophoresis. the major ...199028776819
characteristics of meningitis following transsphenoidal endoscopic surgery: a case series and a systematic literature review.meningitis occurs in 0.8-1.5% of patients undergoing neurosurgery. the aim of the study was to evaluate the characteristics of meningitis after endoscopic endonasal transsphenoidal surgery (eets) comparing the findings retrieved to those highlighted by literature search.201728776163
impact of dust exposure on mixed bacterial cultures and during eukaryotic cell co-culture infections.on a daily basis, humans, and their colonizing microbiome, are exposed to both indoor and outdoor dust, containing both deleterious organic and inorganic contaminants, through dermal contact, inhalation, and ingestion. recent studies evaluating the dust exposure responses of opportunistic pathogens, such as escherichia coli and pseudomonas aeruginosa, revealed significant increases in biofilm formation following dust exposure. in this study, the effects of dust exposure on mixed bacterial cultur ...201728776099
whole-genome sequencing of human clinical klebsiella pneumoniae isolates reveals misidentification and misunderstandings of klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae.klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. the emergence of highly drug-resistant strains is particularly concerning. there has been a recognition and division of klebsiella pneumoniae into three distinct phylogenetic groups: klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae. k. variicola and k. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans tha ...201728776045
antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran.endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran.201728775818
synthesis and structural characterization of silver nanoparticles stabilized with 3-mercapto-1-propansulfonate and 1-thioglucose mixed thiols for antibacterial applications.the synthesis, characterization and assessment of the antibacterial properties of hydrophilic silver nanoparticles (agnps) were investigated with the aim to probe their suitability for innovative applications in the field of nanobiotechnology. first, silver nanoparticles were synthetized and functionalized with two capping agents, namely 3-mercapto-1-propansulfonate (3mps) and 1-β-thio-d-glucose (tg). the investigation of the structural and electronic properties of the nano-systems was carried o ...201628774148
novel durable antimicrobial ceramic with embedded copper sub-microparticles for a steady-state release of copper ions.using pottery clay, porous ceramic stones were molded and then decorated with copper sub-microparticles inside the pores. copper added antimicrobial functionality to the clay-based ceramic and showed ability in disinfecting water. populations of both staphylococcus aureus and klebsiella pneumoniae in contaminated water were reduced by >99.9% in 3 h when exposed to an antimicrobial stone. this antimicrobial performance is attributed to a slow release of copper into water at both room and elevated ...201728773135
automated patterning and probing with multiple nanoscale tools for single-cell analysis.the nano-manipulation approach that combines focused ion beam (fib) milling and various imaging and probing techniques enables researchers to investigate the cellular structures in three dimensions. such fusion approach, however, requires extensive effort on locating and examining randomly-distributed targets due to limited field of view (fov) when high magnification is desired. in the present study, we present the development that automates 'pattern and probe' particularly for single-cell analy ...201728772204
high frequency of the combined presence of qrdr mutations and pmqr determinants in multidrug-resistant klebsiella pneumoniae and escherichia coli isolates from nosocomial and community-acquired infections.plasmid-mediated quinolone resistance (pmqr) determinants combined with mutations in quinolone resistance-determining regions (qrdrs) and clonal dissemination were investigated in 40 fluoroquinolone-resistant klebsiellapneumoniae and escherichiacoli isolates from nosocomial and community-acquired infections. we observed nucleotide substitutions in gyra (ser83ile, val37leu, lys154arg, ser171ala, ser19asn, ile198val, ser83tyr, ser83leu, asp87asn and asp87gly) and parc genes (ser80ile, glu84lys, al ...201728771139
successful ceftazidime-avibactam treatment of mdr-kpc-positive klebsiella pneumoniae infection in a patient with traumatic brain injury: a case report.carbapenem-resistant enterobacteriaceae infections are a serious health care problem, because of the high mortality. carbapenem resistance is mainly caused by carbapenemases production, including klebsiella pneumoniae carbapenemase (kpc). ceftazidime-avibactam is a new cephalosporin/β-lactamase inhibitor combination for the treatment of complicated urinary, intra-abdominal infections, and nosocomial pneumonia caused by gram negative, or other serious gram-negative infections.201728767588
an assessment on the role of endophytic microbes in the therapeutic potential of fagonia indica.natural products of animals, plants and microbes are potential source of important chemical compounds, with diverse applications including therapeutics. endophytic bacteria that are especially associated with medicinal plants presents a reservoir of therapeutic compounds. fagonia indica has been recently investigated by numerous researchers because of its striking therapeutic potential especially in cancer. it is also reported that endophytes play a vital role in the biosynthesis of various meta ...201728764775
antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study.because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ...201728761777
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