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two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
hemoglobin hafnia: alpha 2 (beta 116 (g18) his----gln)2; a new hemoglobin variant mistaken for glycated hemoglobin.isoelectric focusing of hemolysate from patients with diabetes mellitus is routinely performed to measure their level of hba1c (glycated hemoglobin). for a 6 year old boy with diabetes mellitus this analysis showed an hba1c fraction of approx. 50%, which is very unlikely to occur. the possibility of a hemoglobin variant was considered, and by hplc-separation the presence of two different beta-chains was shown. one tryptic fragment was found to deviate from the normal, and amino-acid analysis and ...19883395624
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
hafnia alvei lipopolysaccharides: isolation, sugar composition and sds-page analysis.lipopolysaccharides (lps) of 33 strains of hafnia alvei were isolated and purified. lps content of the dry bacterial mass ranged from 1.2 to 4.5%. all examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some h. alvei strains. sialic acid was present in lps of one strain. d-3-hydroxybutyryl groups also were identified in lipopolysaccharides of 5 ...19883273465
outer membrane protein composition of hafnia alvei.the outer membrane proteins (omp) obtained from 33 strains of hafnia of different origin, were analysed by sds-polyacrylamide gel electrophoresis. about 21 protein bands could be distinguished in omp of each strain. the major proteins of the outer membrane were of apparent molecular weight 34.000 (34k), 37.000 (37k), 39.000 (39k) and 41.000 (41k). on the basis of the electrophoregrams obtained, four groups of strains possessing different composition of major omp were distinguished: (1) 34k and 3 ...19883254120
[the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar].from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified.19883240566
crystallization and purification of the enzyme anthranilate phosphoribosyl transferase.anthranilate phosphoribosyl transferase from the bacterium hafnia alvei has been crystallized. this enzyme is one of a small number that constitute the biosynthetic pathway for tryptophan. large cubic crystals were grown at 4 degrees c by dialyzing away the glycerol from a protein solution that included ammonium sulfate, polyethylene glycol and glycerol. the crystals were much more temperature stable and resistant to x-ray deterioration than a previous, similar crystal form that had included gly ...19883199444
bacterial reduction of fensulfothion and its hydrolysis product 4-methylsulfinyl phenol.oxygen-limited cultures of klebsiella pneumoniae reduced 4-methylsulfinyl phenol to 4-methylthiophenol. a study of the effect of 4-methylthiophenol on the growth of k. pneumoniae revealed that the specific growth rate was retarded by 40% in the presence of 200 micrograms of the phenol per ml. a soil bacterium, hafnia sp., was isolated that could reduce the organophosphorus insecticide fensulfothion to fensulfothion sulfide.19853156557
a conductance medium to distinguish between salmonella and citrobacter spp.published methods for detecting salmonella by conductance methods yield some citrobacter spp. as false positives. a medium is described which, when used in conjunction with selenite-cystine/trimethylamine oxide/mannitol (sc/t/m) resolves this difficulty. it is based on the ability of salmonella to decarboxylate lysine in the presence of selenite (4 g/l), after pre-enrichment in buffered peptone water supplemented with glucose and lysine for 7-17 h. salmonella produced a conductance change of 250 ...19883152806
[comparative activity of habekacin and 4 other aminoglycosides against gram-negative bacilli. evolution of resistance].the activity of 5 aminoglycosides compounds (habekacin, amikacin, gentamicin, netilmicin and tobramycin) was studied by an agar dilution method, against 235 strains of enterobacteriaceae and 146 other gram negative bacilli. 79 to 98% of susceptible strains were observed, according to the aminoglycoside compound. habekacin and amikacin were the most effective, specially against the more frequently resistant bacteria: enterobacter, serratia, hafnia, acinetobacter, pseudomonas aeruginosa. in compar ...19883136424
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...19863088346
[biochemical typing of enterobacter isolated from several clinical materials].a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ...19883078792
hafnia alvei septicemia in an infant with necrotizing enterocolitis.hafnia alvei is an infrequently reported pathogen in children, and its isolation in a newborn is even more unusual. this organism is rarely associated with invasive disease. this article reports the first case of a neonate with necrotizing enterocolitis and subsequent ileal perforation who had h. alvei isolated from both blood and stool.19883057140
further differentiation of enterobacteriaceae by means of siderophore-pattern analysis.by means of a combination of 5 siderophore bioassays using several indicator strains, genera, species and subspecies of enterobacteriaceae can be further differentiated. enterobactin, aerobactin and other siderophores produced can be detected. each strain shows specific pattern which we called siderophore-pattern. it is easy to separate morganella, proteus, providencia, yersinia strains from the genera salmonella, shigella, escherichia coli, enterobacter, citrobacter, klebsiella, serratia. enter ...19882970195
[serious risk of anesthetic systems built over a hafnia-d-system or a 3-way scuttle. erroneous assembling of anesthetic systems might cause fatal pulmonary damage]. 19892911918
antibacterial activity of tigemonam dicholate (sq 30836) and interaction with beta-lactamases of gram-negative bacteria.sq 30836 is an orally absorbed salt of tigemonam, a new monobactam similar to aztreonam in structure and microbiologic properties. when assayed against 400 clinical isolates, tigemonam's activity was similar to that of aztreonam and carumonam. it was highly effective against enterobacteriaceae but showed poor activity against gram-positive organisms. it inhibited 90% of escherichia coli, klebsiella, shigella, yersinia, proteus, providencia, and morganella strains at 0.5 micrograms/ml or less, an ...19892809698
hemoglobinopathies in danish families.based on cases referred for investigation, as well as a questionnaire sent to all medical and pediatric departments in denmark, 48 cases of hemoglobinopathy in 15 families of danish ancestry are reviewed. 18 danes in six families have been identified as having beta-thalassemia, and remarkably one - a homozygote - has beta-thalassemia intermedia requiring treatment with iron-chelation therapy. a further 36 danes in 9 families have a hemoglobin variant: five unstable hemoglobins (volga, niteroi, a ...19892772554
volatile compounds associated with microbial growth on normal and high ph beef stored at chill temperatures.volatile compounds produced by pseudomonas fragi and mixed, natural floras on beef of normal ph (5.5-5.8; glucose greater than 1500 micrograms/g) and high ph (6.3-6.8; glucose less than 10 micrograms/g) included a range of alkyl esters and a number of sulphur-containing compounds including dimethylsulphide but not hydrogen sulphide. production of the last was a property common to the other gram-negative organisms tested viz. hafnia alvei, enterobacter agglomerans, serratia liquefaciens, alteromo ...19892753835
in-vitro interactions of fce 22101 with aminoglycosides against gram-negative rods.agar dilution mics of fce 22101 were measured for 894 consecutively-isolated gram-negative rods from clinical specimens (413 escherichia coli, 104 klebsiella spp., 54 enterobacter spp., 19 citrobacter spp., 131 proteus spp., 43 acinetobacter spp., 9 serratia spp., 3 providencia spp., 4 yersinia spp., 3 hafnia spp. and 111 pseudomonas spp.). excluding pseudomonas spp., 98% of these isolates were susceptible to 8 mg/l fce 22101. resistance (mic greater than 8 mg/l) varied from 11% in serratia spp. ...19892732132
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
o-specific polysaccharides of hafnia alvei lipopolysaccharides isolated from two serologically related strains: atcc 13337 and 1187. a serological and structural study using chemical methods, gas chromatography/mass spectrometry and nmr spectroscopy at 500 mhz.the o-specific polysaccharides of hafnia alvei atcc 13337 standard strain and 1187 strain have been isolated and characterized. by means of 1h-nmr spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where acyl = d-3-hydroxybutyryl, and 3-o-acetylation was to about 66%. the structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were deter ...19892606108
[gram-negative flora of horticultural produce destined for consumption mainly in the raw state].a survey has been carried out to evaluate the recovery of enterobacteriaceae in freshly consumed horticultural products. 64 samples of these vegetables random chosen in different stores in the general vegetable market of ferrara have been examined among the families of compositae (lettuce, prickly lettuce, cabbage lettuce, common chicory, artichoke), umbrelliferae (curly parsley, carrot, celery, fennel), cruciferae (garden cabbage, red radish), liliaceae (onion), and solanaceae (tomato). 654 bac ...19892483908
[taxonomy and systematics of pathogenic microorganisms].the modern state of the problem of the systematics of microorganisms is analyzed and the data on the taxonomy of bacteria belonging to the family enterobacteriaceae are presented. the importance of studies on the taxonomy of microorganisms is emphasized. these studies play a vital role in the development of diagnostic preparations and techniques for the identification of infective agents, as well as in the realization of epidemiological surveillance. much attention is given to the works of sovie ...19862432744
[intergeneric antigenic relation of hafnia and shigella (s. flexneri and s. boydii) bacteria].the o-antigenic relationships between hafnia and shigellae (s. flexneri and s. boydii) have been studied. for the first time the presence of antigenic relationship between hafnia o19, o4, o9, o33, o5, o16, o12, o7, o29, o28, o10, o32, o24, o25, o18, o1, o13, o3, o22, o30, o37, o14, o11, o25, o23, o21, o28, o16, o24, o8, o26, o27 and s. flexneri la, lb, 2a, 2b, 4a, 4b, 6, 5a, 5b, as well as between hafnia o10, o21, o35, o36, o9, o28, o8, o30 and s. boydii 1, 3, 6, 14, 2, 5, 2, 12 have been reveal ...19862431565
[a test for the identification of hafnia alvei bacteria by the color reaction with a complex of 5-nitro-8-hydroxyquinoline + iron ions]. 19862429042
hafnia alvei infection. 19902402660
endogenous endophthalmitis due to salmonella arizonae and hafnia alvei.enteric pathogens rarely involve organs other than those of the gastrointestinal system. we have reported the case of a woman with rheumatoid arthritis who had endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. the infection probably resulted from the use of snake powder as a food seasoner. after appropriate intravenous, intraocular, subconjunctival, topical, and oral antibiotic therapy based on laboratory susceptibility studies, the patient's condition clinically improved, ...19902346566
evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria.a comprehensive phylogenetic tree for virtually the entire assemblage of enteric bacteria is presented. character states of aromatic amino acid biosynthesis are used as criteria, and the results are compared with partial trees based upon sequencing of 16s rrna, 5s rrna, and tryptophan leader peptide. three major clusters are apparent. enterocluster 1 possesses a gene fusion (trpg-trpd) encoding anthranilate synthase: anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase of tryptop ...19902298692
[typical nosocomial infection with an unusual cause: hafnia alvei. report of 2 cases and literature review].the route of infection and the course of two typical nosocomial infections are described in two patients infected with a rare gram-negative bacterium. both patients underwent cardiovascular surgery. they were placed close to each other in the intensive care unit for several days and suffered from pneumonia and from wound infection respectively. in both patients bacterial culture grew hafnia alvei. successful antibiotic treatment was achieved with netilmicin and imipenem. urinary tract, respirato ...19902218242
the normal microbial flora of the outer ear canal in healthy norwegian individuals.the microbial flora of the outer ear canal was determined for 77 healthy individuals (m = 44, f = 33). no growth of any microbe was found in 5% of males and 15% of females. coagulase-negative staphylococci, dominated by staphylococcus epidermidis, were the commonest microbe group found (83% of persons sampled). staphylococcus aureus was found in 7% males and no females. diptheroids were cultured from 32% of the samples. the only gram-negative rod found was hafnia alvei in 4% of individuals. no v ...19902216049
immunogenic and protective properties of outer membrane proteins of hafnia alvei.outer membrane proteins (omp) extracted from antigenically distinct or related strains of hafnia alvei containing defined composition of major proteins proved to be immunogenic. intraperitoneal immunization of mice with a single dose of such preparations protected the animals against homologous and heterologous hafnia strains. the omp preparations were also found to induce protection with varying intensity against escherichia, proteus, shigella and salmonella.19902130804
identification of the bacterium associated with haemorrhagic septicaemia in rainbow trout as hafnia alvei.an unidentified bacterium responsible for epizootic haemorrhagic septicaemic disease in rainbow trout was recently described. this organism exhibited some antigenic similarity to brucella abortus and to yersinia ruckeri and was presumed to belong to the family enterobacteriaceae. further biochemical characterization and dna relatedness studies showed that pathogenic fish strains are hafnia alvei.19902120748
structure of the o-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1h nmr spectroscopy.mild acid hydrolysis of hafnia alvei strain 2 lipopolysaccharide released no o-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with n-acetylneuraminic acid as the reducing terminus. in addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the o-specific polysaccharide chain and the core region, were obtained in minute amounts. on the basis of the sugar and methylation analyses, periodate oxidation, and 1h nm ...19912036370
hafnia alvei, a probable cause of diarrhea in humans.hafnia alvei, a member of the family enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. the isolated strain of h. alvei failed to produce heat-labile or heat-stable enterotoxins or shiga-like toxin i or ii and did not invade hela cells, nor did it cause keratoconjunctivitis (determined by the sereny test) in a guinea pig's eye. the strain, however, induced diarrhea in 8 of 12 adult rabbits ...19912004829
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
induction of the alka gene of escherichia coli in gram-negative bacteria.a broad-host-range plasmid containing a fusion of the alka and lacz genes of escherichia coli was introduced into various aerobic and facultative gram-negative bacteria--33 species belonging to 19 genera--to study the induction of expression of the alka gene by alkylating agents. the bacteria included species of the families enterobacteriaceae, pseudomonadaceae, rhizobiaceae, vibrionaceae, neisseriaceae, rhodospirillaceae, and azotobacteraceae. results obtained show that all bacteria tested, exc ...19911938974
etiologic role of hafnia alvei in human diarrheal illness. 19911937837
l-prolineaminopeptidase activity as a tool for identification and differentiation of serratia marcescens, serratia liquefaciens and hafnia alvei strains.s. marcescens (316 strains), s. liquefaciens (10 strains) and hafnia alvei (20 strains), in contrast to 18 other enterobacteriaceae species, hydrolyzed l-proline-4-nitroanilide within 30 min at 37 degrees c. in this way, rapid identification of these species is possible. the substrate is applied in solution or in a paper disc. the substrate optimum of hafnia alvei strains proved to be 16 times lower than that of the serratia spp. application of a tenth of the substrate concentration necessary fo ...19911930558
esp3i--a novel type iis restriction endonuclease from hafnia alvei that recognizes the sequence 5'-cgtctc(n)1/5-3'. 19911923776
expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco.a possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. the ldcgene from hafnia alvei was therefore (a) placed under the control of the 1' promoter of the bidirectional tr promoter from agrobacterium tumefaciens ...19911883998
[clinical significance of the isolation of hafnia alvei from the feces of children with acute gastroenteritis]. 19911822161
plasma desorption mass spectrometry as a tool in characterization of abnormal proteins. application to variant human hemoglobins.we here report the application of plasma desorption mass spectrometry in combination with reversed-phase high-performance liquid chromatography and automatic edman sequencing for the characterization of hemoglobin variants. by use of plasma desorption mass spectrometry to obtain molecular weight information of purified globin peptides it is possible to minimize the number of candidate positions for substitutions allowing an optimal use of automatic edman degradation. each variant can be characte ...19911812782
what is the diagnostic value of beta-d-glucuronidase (bdg) activity of bacteria using fluorocult ecd agar for their cultivation?a total of 1510 strains from 15 genera of gram-negative and gram-positive bacteria were studied. more than 94% of 327 escherichia coli strains showed beta-d-glucuronidase (bdg) activity. seventeen serotypes from 170 e. coli o serogroup representatives were negative. relationship between the existence of bdg positive and negative e. coli strains in the same serogroup or serotype has not been observed. the rate of bdg positivity was 42% among salmonella arizonae strains and 42.2% among shigella st ...19911805501
serological characterization of lipopolysaccharides of hafnia alvei 1, 17, 31, 32, 38 and 39.lipopolysaccharides of hafnia alvei strains 1, 17, 31, 32, 38 and 39 indicate strong cross-reactivity in passive hemagglutination. all lipopolysaccharides were examined by rocket immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting.19911804049
identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test.we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ...19911774276
[hafnia alvei septicemia with shock and dic in an adult with postoperative lung cancer].in japan, we experienced the first case of hafnia alvei septicemia with shock and disseminated intravascular coagulation (dic) in an adult with postoperative lung cancer. a 63 year-old male, who had been followed up in our department since 1987, was admitted to our hospital with the complaints of fever, hemoptysis and dyspnea on june 25, 1989. after admission, he was treated with sulbactam/cefoperazone 4 g/day intravenously for suspicion of respiratory-tract infection. after antibiotic administr ...19911761901
structural studies on hafnia alvei 114-60 o-antigen.the structure of hafnia alvei 114-60 o-antigen has been established using sugar and methylation analyses as well as 1h-nmr spectroscopy. the results obtained proved that the repeating unit of hafnia alvei 114-60 o-antigen is identical to that of hafnia alvei atcc 13337 standard strain and has the following structure: [formula: see text]19901718234
evolution of the ferric enterobactin receptor in gram-negative bacteria.using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ...19911717434
o-specific polysaccharide of hafnia alvei lipopolysaccharide isolated from strain 1211. structural study using chemical methods, gas-liquid chromatography/mass spectrometry and nmr spectroscopy.the hafnia alvei strain 1211 o-specific polysaccharide is composed of 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactose, n-acetyl-d-galactosamine, n-acetyl-d-glucosamine and d-glucose (1:1:2:2). on the basis of sugar and methylation analyses, smith degradation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, the polysaccharide was shown to be an o-acetylated polymer of the repeating hexasaccharide unit, ----2d(4-oac)fucp3nacyl beta 1----6dglcpnac alpha 1---- (dglcp beta 1----3)4 ...19911716201
[the etiologic structure of acute intestinal infections caused by opportunistic microorganisms (irkutsk, 1984-1988)].the authors analyze the results of bacteriologic studies carried out in patients with intestinal infections at the laboratory of the sanitary epidemiologic center of the town of irkutsk over 1984-1988. the records of 24,490 case histories show infections induced by opportunistic microflora in 37 percent of cases. the most frequent etiologic agents were staphylococcus aureus and enterobacteriaceae, genera: proteus, enterobacter, citrobacter, klebsiella, hafnia. analysis of the incidence of acute ...19911715437
[the identification of lactose-negative enterobacteria].study of the morphologic and enzymatic characteristics of 10 strains of lactose-negative enterobacteria, sent to the l. a. tarasevich state institute for control and standardization as b. paracoli in order to determine their taxonomic status in accordance with the 1974 and 1984 bergey classifications has referred 9 of these strains to hafnia alvei.19901705611
the structure of the biological repeating unit of the o-antigen of hafnia alvei o39.on mild acid-catalysed degradation of the lipopolysaccharide from hafnia alvei o39 followed by gel filtration of sephadex g-50, the o-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 o-antigen repeating-units. on the basis of sugar and methylation analyses, 13c-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13c-n.m.r. analysis of the smith-degraded polysaccharide, it was conclude ...19901703456
in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin).the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ...19911664418
the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...19911657086
acute bacterial gastroenteritis caused by hafnia alvei. 19921623093
[colitis and hafnia alvei]. 19921602859
ru 29 246, the active compound of the cephalosporin-prodrug-ester hr 916. i. antibacterial activity in vitro.the aminothiazolyl-cephalosporin ru 29 246 is the active metabolite of the prodrug-pivaloyl-oxyethyl-ester hr 916. ru 29 246 in vitro activity includes a wide range of clinically relevant bacterial pathogens. against methicillin-sensitive staphylococci ru 29 246 (mic90 of 0.25 approximately 2 micrograms/ml) was clearly more active than cefaclor, cefuroxime, cefpodoxime, cefixime and ceftibuten, but slightly less active than cefdinir. ru 29 246 inhibited hemolytic streptococci of the serogroups a ...19921592683
nucleotide sequence of the escherichia coli cad operon: a system for neutralization of low extracellular ph.lysine decarboxylase of escherichia coli has been the subject of enzymological studies, and the gene encoding lysine decarboxylase (cada) and a regulatory gene (cadr) have been mapped. this enzyme is induced at low ph in the presence of lysine and achieves maximal level under anaerobic conditions. the induction of lysine decarboxylase increases the ph of the extracellular medium and provides a distinctive marker in tests of clinical strains. we report the sequence of the cad operon encoding lysi ...19921556085
in vitro activity and beta-lactamase stability of ljc 10,627.the in vitro activity of ljc 10,627, a new carbapenem, was compared with those of imipenem, cefotaxime, ceftazidime, and gentamicin. ljc 10,627 inhibited 90% of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter agglomerans, enterobacter cloacae, hafnia alvei, citrobacter freundii, citrobacter diversus, proteus mirabilis, morganella morganii, proteus rettgeri, serratia marcescens, pseudomonas cepacia, salmonellae, shigellae, aeromonas, and yersiniae ...19921510436
analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry.fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ...19921447059
sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic escherichia coli and hafnia alvei.seven strains of hafnia alvei isolated from diarrhoeal stools of children resembled enteropathogenic escherichia coli (epec) in that they produced attaching-effacing (ae) lesions in rabbit ileal loops and fluorescent actin staining in infected hep-2 cells. in addition, a dna probe from a chromosomal gene required by epec to produce ae lesions, hybridised to chromosomal dna from all seven h. alvei strains. these findings indicate that there is a sharing of virulence-associated properties at the p ...19921433251
alw26i, eco31i and esp3i--type iis methyltransferases modifying cytosine and adenine in complementary strands of the target dna.the specificity of three dna methyltransferases m.alw26i, m.eco31i and m.esp3i, isolated from acinetobacter iwoffi rfl26, escherichia coli rfl31 and hafnia alvei rfl3+, respectively, was determined. all the enzymes methylate both strands of asymmetric recognition sites yielding m5c in the top-strand and m6a in the bottom-strand, as below: 5'-gtm5ctc 5'-ggtm5ctc 5'-cgtm5ctc 3'-cm6agag 3'-ccm6agag 3'-gcm6agag (m.alw26i) (m.eco31i) (m.esp3i) they are the first members of type iis methyltransferases ...19921408816
the structure of the o-specific polysaccharide from hafnia alvei strain 38 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide from h. alvei strain 38 has been established by nmr spectroscopy (13c and 1h) and methylation analysis to have the repeating unit-->4)-beta-d-manpnac-(1-->4)-alpha-d-glcpnac(1-->.19921394327
the structure of a glycerol teichoic acid-like o-specific polysaccharide of hafnia alvei 1205.the o-specific polysaccharide of hafnia alvei 1205 contained d-glucose, d-galactose, 2-acetamido-2-deoxy-d-glucose, 4-acetamido-4,6-dideoxy-d-glucose (qui4nac), glycerol, phosphate, and o-acetyl groups. on the basis of 1d and 2d shift-correlated homonuclear and 13c-1h heteronuclear nmr spectroscopy, methylation analysis, smith degradation, and dephosphorylation with hydrofluoric acid, it was concluded that the o-antigen was a partially o-acetylated teichoic acid-like polysaccharide having the fo ...19921394318
sheen formation and growth response of groundwater bacteria to reduced oxygen concentrations during incubation of m-endo medium.in vitro pure-culture studies were conducted to assess growth and sheen formation of groundwater bacteria on m-endo medium incubated under reduced oxygen concentrations (0, 4, 8, 12, and 16%). coliform and noncoliform bacteria were isolated from 17 untreated, rural groundwater supplies on m-endo medium. all 16 coliform isolates tested were capable of sheen formation at oxygen concentrations of 4% or greater, yet some of these same isolates (enterobacter aerogenes, enterobacter cloacae, and hafni ...19921393829
[occurrence of p.fimbrii in strains from selected genera of enterobacteriaceae].this study was aimed at recognition of frequency of occurrence of p fimbriae in strains of escherichia coli isolated from samples of feces of children with symptoms of diarrhoea and at search of these adhesions in strains representing other than escherichia genera of enterobacteriaceae strains. one hundred forty laboratory strains were investigated. they belonged to genus citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, salmonella, shigella, and yersinia. also wer ...19921363960
epidemiological role of arthropods detectable in health facilities.a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ...19921350604
humoral response in mice immunized with outer membrane proteins of hafnia alvei. protective activity of anti-omp-antibodies.intraperitoneal immunization of mice with outer membrane proteins (omp) of hafnia alvei induced in the animals a synthesis of specific antibodies. the antibody levels, determined by elisa test, were found to be relatively low in the sera of mice immunized with a single dose (5 micrograms) of omp and after a second immunization. however, they were higher in mice immunized with three doses of omp. the antibodies were present in circulation for a relatively short time after immunization. serum cont ...19921340186
some biological properties of outer membrane proteins of hafnia.outer membrane proteins (omp) isolated from four antigenically different strains of hafnia alvei were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 doses for the studied preparations determined on inbred mice were 18, 20, 28 and 34 mg/kg. these differences in the toxicity of the preparations were reflected in manifestation of shwartzman reaction; more toxic preparations induced strongest necrohemorrhagic ...19921299170
studies on immunity in mice immunized with outer membrane proteins of hafnia.nonspecific protection induced in mice after administration outer membrane proteins of hafnia alvei against infection with homologous and heterologous bacteria was transferred into other mice with lymphocytes isolated from spleens of mice immunized with outer membrane proteins. it was also found that mice sensitized with outer membrane proteins derived from h. alvei or with living bacteria induced in animals delayed hypersensitivity (dth) in homologous and heterologous systems. the observed type ...19921299169
[frequency of detecting bacteria of the genera citrobacter and hafnia in different contingents of examined adults].a study of the incidence of bacteria of the citrobacter and hafnia genus in adults permitted to establish a greater occurrence of the mentioned microbes in the patients with various acute intestinal diseases in comparison with the healthy ones. bacteria of the citrobacter and hafnia genus were revealed in the patients with acute intestinal diseases of obscure etiology, which often were diagnosed as gastroenteritis, enteritis, etc. bacteria of the citrobacter and hafnia genus were revealed in the ...19751236718
effects of chemotherapeutics on bacterial ecology in the water of ponds and the intestinal tracts of cultured fish, ayu (plecoglossus altivelis).drug-resistant gram-negative bacilli conferred with r factors were isolated with high frequencies from the intestinal tracts of ayu (plecoglossus altivelis) cultured in ponds, in which chemotherapeutics had often been used, and with relatively low frequencies from ayu which received no administration of chemotherapeutics. drug-resistant bacteria were also isolated at low frequencies from the intestinal tracts of wild ayu in rivers, as well as from the water of ayu-culturing ponds and some of the ...19751160204
bactericidal and opsonic activities of normal sheep serum against gram-negative bacteria.the bactericidal and opsonic activities of normal sheep serum against 9 smooth and 4 rough strains of gram-negative bacteria were measured. three smooth strains-escherichia coli 3662, salmonella typhimurium, and salmonella gallinarum-were resistant to the bactericidal action of normal sheep serum with or without complement. six smooth strains-e coli o78:k80(b), salmonella arizonae, proteus inconstans, klebsiella pneumoniae, salmonella stanley, and salmonella abortus-equi-were moderately sensitiv ...19751098532
chronic polymicrobial bacteremia.a 13-year-old girl had chronic polymicrobial bacteremia with enterobacter hafnia and enterobacter agglomerans in the absence of any demonstrable underlying illness, use of immunosuppressive drugs, or discovery of portal of entry of the bacteria. she was treated successfully with a prolonged course of carbenicillin and aminoglycoside antibiotics which were tolerated well. the only side effect of the therapy was a transient episode of vestibular dysfunction which was reversible following cessation ...19751078798
[nationwide fire inspection following the hafnia catastrophe shows: a large number of nursing homes are purely firetraps]. 19751040338
[identification of conditionally pathogenic enterobacteria in laboratory practice in the diagnosis of acute intestinal diseases].identification of 361 cultures isolated from patients suffering from various acute intestinal diseasesand from persons who had sustained them, as well as from contacts and persons examined prophylactically with the use of various biochemical tests showed that ty their taxonomic properties the cultures were referred to conditionally-pathogenic representatives of enterobacteriaceae of the corresponding genera:citrobacter, hafnia, klebsiella, proteus, providencia. serological typing of the strains ...19761007731
the hafnia a circuit: a valveless non-polluting anaesthetic system. 1976998159
[taxonomic importance of fermentation balances of enterobacteriaceae (author's transl)].fermentation balances have been studied on 66 strains from 9 taxa of enterobacteriaceae. in addition, 6 strains of aeromonas have been investigated. among these fermentation balances, 7 types could be distinguished. it was shown, that the taxa of enterobacteriaceae differed with respect to the balance types involved. this is true especially for vp-positive taxa. for example, hafnia, the klebsiella-enterobacter-serratia group, enterobacter cloacae and erwinia are to some extend characterized by d ...1976998036
[biochemical characteristics of strains from the citrobacter and hafnia genera and their laboratory identification].a study of biochemical properties of 176 strains of coprocultures isolated from the patients and persons who sustained various acute enteric diseases, and also from contacts and persons examined for prophylaxis showed that they corresponded to the taxonomic definition of the citrobacter and hafnia genus. in connection with the similarity by biochemical properties between bacteria of citrobacter and salmonella genus and bacteria of hafnia and shigella genus it is of expedience in their identifica ...1976961244
the 600 gram co2 absorption canister: an experimental study.model investigations were performed on a canister containing 600 g of sodasorb indicator soda lime in a transparent lime compartment used in the hafnia circle. the co2 input was 300 ml per min. carbon dioxide will be efficiently eliminated from the gas mixture conveyed to the patient connection in this system for at least 4 h, irrespective of the rate of fresh gas flow. there was proportionality between the fresh gas inflow and the duration of effective co2 elimination. the relative efficacy of ...1977910616
serologic relationship of fimbriae among enterobacteriaceae.the existence of serological relationship of fimbriae among serotypes of the same genus and among genera of enterobacteriaceae was determined by agglutination and absorption procedures. it was found that fimbriae of different serotypes of the same genus and among the genera a) escherichia, shigella and klebsiella, and b) salmonella, arizona and citrobacter were more or less antigentically related. no relationship was found among fimbriae of the edwardsiella, enterobacter, hafnia, serratia, prote ...1977880000
[isolation of bacteria of the germs hafnia from chickens and poultry maids and their virulence]. 1977865327
[on the growth of various enterobacteriaceae, pseudomonas aeruginosa and alkaligenes spec. in distilled water, de-ionized water, tap water, and mineral salt solution (author's transl)].the behaviour of 12 strains of gram-negative bacteria was analysed in media having a low content of nutrients. sterile tap water, de-ionized water, aqua bidestillata and mineral salt solution were used. it was found that serratia marcescens, pseudomonas aeruginosa, enterobacter, klebsiella and citrobacter are those with the most frequently observed growth. in the mineral salt solution survived also providencia, e. coli, erwinia, hafnia, and alkaligenes and reached max. colony numbers of some 100 ...1976828367
[development of a differentiation scheme for hafnia using the h antigen].the authors suggest a differentiation scheme for hafnia bacteria by the h-antigen, including 36 varieties of the flagellar antigen. a collection of h-test-strains which can be used in elaboration of technology of preparation of hafnia h-sera was determined.1976795242
[characteristics of the enterobacteria strains isolated from the urine in urinary tract infections].study of biochemical properties of 421 strains of enterobacteria permitted to refer them to the following taxonomic groups of which e. coli constituted 83,4 +/- 3.6%, enterobactercloacae--97 +/- 3.0%, klebsiella 4.0 +/- 2.0%. enterobacter aerogenes, enterobacter hafnia, citrobacter, proteus rettgeri, proteus vulgaris were revealed in individual cases (0.2-1.0%). as a result of serological typing there were revealed 105 serological types of escherichia differing by 55 o-antigen variants, 26 h-ant ...1978747014
enterobacter hafnia meningitis. 1978722430
[resistance to antibiotics of conditionally pathogenic enterobacteria of the genera, citrobacter and hafnia].antibiotic sensitivity of 363 strains of citrobacter and 124 strains of hafnia isolated from patients with acute intestine infections and healthy persons was studied. it was found that 44.6 per cent of the citrobacter isolates and 36.3 per cent of the hafnia isolates were resistant to one or more antibiotics. different sensitivity of the bacteria of these 2 genera of the conditionally pathogenic enterobacteria to definite antibiotics was shown. the resistant strains of citrobacter to negramon, m ...1978655688
[study of the hafnia isolated from honey]. 1978654096
flow requirements in the hafnia modifications of the mapleson circuits during spontaneous respiration.the mapleson a, b, c and d circuits can be changed into non-polluting circuits by employing continuous gas evacuation directly from the circuit, via an ejector flowmeter (jørgensen 1974); mapleson a and c circuits with this modification have been described previously as the hafnia a and c circuits (christensen 1976, thomsen & jørgensen 1976). if evacuation from a closed reservoir is employed, total removal of the expired and surplus gases from the operating theatre is obtained (jørgensen & thoms ...1978636803
[microfloral study of bull seminal fluid stored at low temperatures].hundred twenty three samples of bull semen fluid frozen at 196 degrees c including 83 plastic ampules, 20 granules and 20 plastic straws obtained from the containers of the insemination stations of 10 farms from the sofia district were investigated. two hundred twelve strains were isolated and identified as: escherichia coli--25 strains, hafnia--16 strains, citrobacter, enterobacter and proteus mirabilis--9 strains of each. the remaining gram-negative genera and species were more rarely encounte ...1979545852
[diagnostic value of the acid production from galacturonate to differentiate strains of "salmonella" sub-genus i and monophasic sub-genus iii from the other "salmonella" strains belonging to sub-genera ii, diphasic iii, iv, "citrobacter" and "hafnia alvei" (author's transl)].two methods were compared: (1) the ability to use sodium glacturonate as source of carbon and energy in defined medium; (2) the ability to produce acid by fermentation of this substrate in peptone water with phenol red. results of growth on, acid formation from, galacturonate are identical. salmonella strains of sub-genus i and monophasic serotypes of sub-genus iii do not use galacturonate. salmonella of sub-genera ii and iv as well as diphasic serotypes of sub-genus iii use galacturonate. glact ...1979533071
differentiation of selected enterobacteriaceae by pyrolysis-gas-liquid chromatography.pyrolysis-gas-liquid chromatography was used to differentiate selected species of enterobacteriaceae. individual cultures of salmonella typhi, hafnia alvei, and proteus vulgaris, and 12 strains of yersinia enterocolitica were grown in nutrient broth. after harvest and lyophilization, the bacterial samples were pyrolyzed at 900 degrees c, and their volatile fractions were separated on a 50-m capillary column coated with carbowax 20m. the resulting pyrolysis elution patterns (pyrograms) of the fou ...1979393169
[transmission of plasmid rldrd19 from e. coli to hafnia].investigations carried out demonstrated a possibility of transmission of plasmid rldrd19 from e. coli to hafnia. the incidence of the plasmid transmission varied from 10(-7) to 10(-9) and depended on the properfies of hafnia strains. tra-operon of plasmid rldrd19 in the hafnia 614 strain functioned with the same efficacy as in e. coli. plasmid rldrd19 in hafnia was unstable and was eliminated from the cells in case of storage at low temperatures. as shown, plasmid rldrd19 was under strict replic ...1978354287
[study of the role of conditionally pathogenic enterobacteria in acute intestinal diseases].conditionally pathogenic enterobacteria were isolated in 34.2% of the patients with acute intestinal diseases (1559 cases examined in all). such representatives of enterobacteria as klebsiella, hafnia, citrobacter of 4, 8, and 22 serological groups. proteus mirabilis of 6, 10, 13, 26, and 28 serological groups, and proteus morganii mostly played the etiological role. in some of the patients conditionally pathogenic microbes only aggravated the main disease, taking part in the development of dysb ...1977335739
[enterobacteria erroneously identified as salmonella in human faeces (author's transl)].to differentiate citrobacter freundii, enterobacter hafniae, proteus mirabilis and alteromonas putrefaciens from salmonella strains isolated in faeces, the author recommends the use of "hajna-kligler" medium, "nitrate-motility-mannitol" medium, "urease-indole-trytophane desaminase" medium and glycerol peptonated water. the lysis of salmonella and e. hafniae strains by specific phages (felix and callow o: 1 salmonella phage; guinee and valkenburg hafnia phage) is emphasized.1977335345
comparison of anti-pollution modifications of mapleson a and d anaesthetic systems.hafnia a and hafnia d anaesthetic systems are non-polluting modifications of the mapleson a and d systems. the excess gas is vented in the hafnia systems directly and continuously via a calibrated ejector flowmeter. sixteen adult patients anaesthetized for abdominal surgery were ventilated and either hafnia a or d systems at six different fresh gas flows. no difference between the hafnia a and d systems could be demonstrated in the values of arterial carbon dioxide tension measured with the same ...1979159068
a simple method of monitoring carbon dioxide output in anaesthetized patients.the mean co2 output during anaesthesia in paralyzed patients can be monitored by continuous capnographic analysis of the total exhaled gases, the latter being mechanically integrated by pumice canisters. the gas is evacuated from the hafnia a circuit via an ejector flowmeter. the results are not influenced by the flow rates employed.1977143865
arterial carbon dioxide tensions during anaesthesia with manual ventilation. a descriptive study of the effects of various non-polluting circuits.in 660 supine, intubated and anaesthetized, healthy patients scheduled for various elective surgical procedures, the distribution of arterial carbon dioxide tension (paco2) was investigated during manual non-monitored ventilation. the study comprised six equal groups: group 1: ventilation with a circle circuit absorber system; group 2: ventilation with the hafnia a circuit using a total fresh gas flow (fgf) of 100 ml . kg-1 . min-1; groups 3-6: ventilation with a hafnia d circuit with fresh gas ...197939415
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