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lactate dehydrogenase release assay from vero cells to distinguish verotoxin producing escherichia coli from non-verotoxin producing strains.the vero cell assay presently used for virulence testing of verotoxigenic escherichia coli (vtec) requires at least 48-96 h where cytotoxicity effects are examined under a microscope. here, a complimentary rapid assay was developed that measures endogenous lactate dehydrogenase (ldh) release from vero or hep-2 cells as an indicator of cytotoxicity. toxin preparations from 24 vtec strains induced 36-89% ldh from vero cells and 15-62% ldh from hep-2 cells in 12-16 h. a verotoxin-positive but enter ...200111118652
characterisation of hafnia alvei isolates from human clinical extra-intestinal specimens: haemagglutinins, serum resistance and siderophore synthesis.extra-intestinal hafnia alvei isolates are rarely considered to be pathogenic. to investigate whether such strains are able to produce virulence factors, a total of 70 clinical h. alvei isolates was compared with clinical extra-intestinal isolates of other members of the enterobacterial tribe klebsiellae (kiebsiella pneumoniae, enterobacter cloacae, serratia marcescens). whereas mannose-sensitive haemagglutination (msha) was less common in h. alvei (59%) than in k. pneumoniae (86%) and e. cloaca ...200111232764
identification and characterization of integron-mediated antibiotic resistance among shiga toxin-producing escherichia coli isolates.a total of 50 isolates of shiga toxin-producing escherichia coli (stec), including 29 o157:h7 and 21 non-o157 stec strains, were analyzed for antimicrobial susceptibilities and the presence of class 1 integrons. seventy-eight (n = 39) percent of the isolates exhibited resistance to two or more antimicrobial classes. multiple resistance to streptomycin, sulfamethoxazole, and tetracycline was most often observed. class 1 integrons were identified among nine stec isolates, including serotypes o157: ...200111282605
structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c.the following structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c was established using sugar and methylation analyses and nmr spectroscopy, including 2d cosy, tocsy, noesy, and 1h, 13c heteronuclear single-quantum coherence (hsqc) experiments: (struture: see text). the main d-mannan chain of the polysaccharide studied has the same structure as the o-specific polysaccharide of escherichia coli o9, klebsiella pneumoniae o3, and hafnia alvei pcm 1223.200111454340
comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development.bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ...200111516937
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
[spontaneous bacterial peritonitis caused by hafnia alvei]. 200111333694
structural and serological studies on hafnia alvei o-specific polysaccharide of alpha-d-mannan type isolated from the lipopolysaccharide of strain pcm 1223.on the basis of chemical and methylation analyses, one- and two-dimensional (1)h- and (13)c-nmr spectroscopy, including cosy, tocsy, noesy and (1)h, (13)c hsqc experiments, a neutral o-specific polysaccharide isolated from hafnia alvei strain pcm 1223 lipopolysaccharide (lps) was found to be an alpha-mannan composed of pentasaccharide repeating units having the following structure:-->3)-alpha-d-manp-(1-->3)-alpha-d-manp-(1-->2)-alpha-d-manp-(1-->2)-alpha-d-manp-(1-->2)-alpha-d-manp-(1-->. immuno ...200111335142
[pneumonia caused by hafnia alvei in a patient infected with human immunodeficiency virus]. 200111256254
structure of the o-specific polysaccharide of hafnia alvei pcm 1196.an acidic o-specific polysaccharide was isolated from hafnia alvei pcm 1196 lipopolysaccharide and studied by sugar and methylation analyses along with one- and two-dimensional 1h and 13c nmr spectroscopy, including noesy and hmbc experiments. the following structure of the pentasaccharide repeating unit was established: -->4)-alpha-d-galpa-(1-->3)-beta-d-glcpnac-(1-->2)-beta-d-galp-(1-->6)-alpha-d-glcp-(1-->6)-alpha-d-glcpnac-(1-->.200111269405
[bilateral lung tuberculosis and over-infection by hafnia alvei]. 200212481504
evolutionary relationship of alw26i, eco31i and esp3i, restriction endonucleases that recognise overlapping sequences.type ii restriction endonucleases (enases) have served as models for understanding the enzyme-based site-specific cleavage of dna. using the knowledge gained from the available crystal structures, a number of attempts have been made to alter the specificity of enases by mutagenesis. the negative results of these experiments argue that the three-dimensional structure of dna-enase complexes does not provide enough information to enable us to understand the interactions between dna and enases in de ...200212172806
[occurrence of beta-lactamase types esbl and ampc among gram-negative rods isolated from food].the aim of the study was to isolate extended spectrum beta-laktamases (esbl) and chromosomal beta-laktamases ampc producing strains from food products. a total of 739 gram-negative bacteria were tested with double disc diffusion method using cefotaxim, ceftasidim and amoxycillin with clavulanic acid. one strain producing esbl and belonging to stenotrophomonas maltophilia was detected from the ice cream (0.14% of all strains). from different food products a total of 14 microorganisms (1.9%) havin ...200212185694
[enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital].to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms.200211939120
the structure of the o-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of hafnia alvei that has characteristics of a new species under the genus escherichia.the structure of the o-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in bangladesh was studied with sugar, and methylation analysis, nmr spectroscopy, mass spectrometry and partial acid hydrolysis. the strain was first designated as hafnia alvei, but later found to be a possible new species in the genus escherichia. two different polysaccharides were detected, a major and a minor one. the structure of the major polysaccharide is given below, while the structure of the ...200212084070
an analysis of human pathogens found in horse/mule manure along the john muir trail in kings canyon and sequoia and yosemite national parks.to determine the prevalence of microorganisms that are potentially pathogenic for humans in horse/mule manure along the john muir trail (jmt).200212092962
phenotypic and genotypic properties of the genus hafnia.the present study characterised 73 hafnia alvei isolates and five escherichia isolates (originally identified as h. alvei) isolated from cases of diarrhoeal disease by the international centre for diarrhoeal disease research branch (icddrb) in bangladesh. based upon the hydrolysis of arbutin and aesculin and the fermentation of salicin and d-arabinose, four distinct biotypes could be recognised among the 73 h. alvei isolates tested; biotype 1 (d-(-)-arabinose-positive only) accounted for 75% of ...200212132774
theoretical evaluation of zirconia and hafnia as gate oxides for si microelectronics.parameters determining the performance of the crystalline oxides zirconia (zro2) and hafnia (hfo2) as gate insulators in nanometric si electronics are estimated via ab initio calculations of the energetics, dielectric properties, and band alignment of bulk and thin-film oxides on si (001). with their large dielectric constants, stable and low-formation-energy interfaces, large valence offsets, and reasonable (though not optimal) conduction offsets (electron injection barriers), zirconia and hafn ...200212484834
modification of niven's medium for the enumeration of histamine-forming bacteria and discussion of the parameters associated with its use.the objective of this study was to improve niven's medium (nm) for the optimized enumeration of histamine-forming bacteria (hfb). the parameters modified related to solidification of the agar at low ph values (ph 5.3 to 5.8), incubation time (24, 48, and 72 h) and temperature (30 and 37 degrees c), number of colonies developed on the plate to allow enumeration of hfb, and color differentiation. strains of hfb, morganella morganii, klebsiella pneumoniae, and hafnia alvei were examined for their a ...200211899055
comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.a 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees c and transfer to 44 +/- 1 degrees c for 22 h) using fecal coliform agar (fca) was compared with the 24-h standardized violet red bile lactose agar (vrbl) method. fca and vrbl have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as hafnia alvei, which could form typical colonies on fca and vrbl. recovery of cold-stressed ...200211916678
mechanism of interstitial oxygen diffusion in hafnia.we have performed density functional calculations of oxygen incorporation and diffusion in monoclinic hafnia (hfo2) for a range of oxygen charge states. the calculations demonstrate that oxygen favors atomic incorporation and that o2- is the most stable species. we find that oxygen interstitials diffuse via exchange with lattice oxygen sites in hafnia, and that o- species have the smallest diffusion barrier.200212485083
[pathogenicity islands in opportunistic enterobacteria].the presence of fragments of genomes hlya, hlyb, papah, papc, sfag, sfaa and kps mt, associated with the pathogenicity islands of escherichia coli, in clinical strains of other genera of the family enterobacteriaceae, has been experimentally evaluated with the use of pcr. the presence of dna fragments specific to the known genes of the pathogenicity clusters of e. coli in representatives of the genera citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, serratia and y ...200212506618
gas formation in ground beef chubs due to hafnia alvei is reduced by multiple applications of antimicrobial interventions to artificially inoculated beef trim stock.gas-forming microorganisms were isolated from gas-swollen ground beef chubs obtained from a commercial source and were phenotypically identified as hafnia alvei. in in situ experiments, the isolated h. alvei strains produced gas in inoculated irradiation-sterilized ground beef chubs. a five-strain cocktail of h. alvei isolates was inoculated on beef trim. the inoculated beef trim samples were treated with either a water wash (w) at 65 psi for five passes (a pass refers to the application of succ ...200212380755
ion-assisted deposition of moisture-stable hafnium oxide films for ultraviolet applications.a design-of-experiments statistical approach was taken to determine the optimum ion gun operating parameters for the deposition of moisture-stable, low-absorbing hafnium oxide films by ion-assisted electron-beam evaporation. factors identified as affecting the quality of hafnia films were chamber pressure, deposition rate, ion gun source gas composition, and ion gun current. both oxygen and argon were used as source gases. high and low levels of the factors were chosen on the basis of our experi ...200212064403
evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies.phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ...200211931166
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1546.an acidic o-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of hafnia alvei pcm 1546 is composed of d-gal, d-glc, d-glca, d-galnac and o-acetyl groups in the ratios 1:1:1:2:1.6. on the basis of sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [see equation in text].200314505884
electron energy-loss spectroscopy study of thin film hafnium aluminates for novel gate dielectrics.we have used conventional high-resolution transmission electron microscopy and electron energy-loss spectroscopy (eels) in scanning transmission electron microscopy to investigate the microstructure and electronic structure of hafnia-based thin films doped with small amounts (6.8 at.%) of al grown on (001) si. the as-deposited film is amorphous with a very thin (approximately 0.5 nm) interfacial siox layer. the film partially crystallizes after annealing at 700 degrees c and the interfacial sio2 ...200312694419
biochemical properties of a newly described escherichia species, escherichia albertii.five strains of a newly described escherichia species, escherichia albertii, were extensively characterized by conventional biochemical methods and by commercial identification panels. e. albertii is an indole-negative species that ferments d-mannitol but not d-xylose. because these strains are not included in the databases of commercial systems at present, they were most often identified as hafnia, salmonella, escherichia coli, or, on one system (microscan dried overnight panels), yersinia ruck ...200314532241
[nosocomial pneumonia caused by hafnia alvei]. 200314624659
caseinolysis in cheese by enterobacteriaceae strains of dairy origin.to study the effect of enterobacteriaceae strains of dairy origin on caseins under cheese manufacture and ripening conditions.200314633113
detection of morganella morganii, a prolific histamine former, by the polymerase chain reaction assay with 16s rdna-targeted primers.a polymerase chain reaction (pcr) assay for the rapid and sensitive detection of the most prolific histamine former, morganella morganii, was developed. 16s rdna targeted pcr primers were designed, and the primer specificity and sensitivity of the pcr assay were evaluated. the 16s rdna sequence (1,503 bp) for m. morganii showed 95% identity to those for enteric bacteria, i.e., enterobacter spp., klebsiella spp., citrobacter spp., hafnia alvei, proteus spp., and providencia spp. the unique primer ...200312929824
[clinical and microbiological features of acute enteric mixed infection caused by hafnia alvei and rotavirus].the data obtained in the clinical and laboratory study of 72 hospitalized patients with acute enteric infection are presented. the observed outbreak was caused by h. alvei producing heat-stable enterotoxin. the role of this etiological agent is also confirmed by simultaneous occurrence of the disease after using the same foodstuff, a short incubation period, the severity of the course of the disease with pronounced symptoms of neurotoxicosis, a high detection rate of h. alvei in material taken f ...200312886610
evaluation of increased incubation temperature and cefixime-tellurite treatment for the isolation of escherichia coli o157:h7 from minced beef.to improve enrichment and isolation of escherichia coli o157:h7, this study evaluated increased incubation temperature and cefixime-tellurite (ct) on five strains of each of the following bacteria, e. coli, hafnia alvei, enterobacter spp., citrobacter freundii and e. coli o157:h7, and two strains of e. coli o157:nh7. these were grown in pure culture in lst broth with varying cefixime-tellurite concentrations. a range of incubation temperatures from 37 to 46 degrees c was investigated for the inh ...200312927704
a molecular phylogeny of enteric bacteria and implications for a bacterial species concept.a molecular phylogeny for seven taxa of enteric bacteria (citrobacter freundii, enterobacter cloacae, escherichia coli, hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, and serratia plymuthica) was made from multiple isolates per taxa taken from a collection of environmental enteric bacteria. sequences from five housekeeping genes (gapa, groel, gyra, ompa, and pgi) and the 16s rrna gene were used to infer individual gene trees and were concatenated to infer a composite molecular phylogen ...200314640415
escherichia albertii sp. nov., a diarrhoeagenic species isolated from stool specimens of bangladeshi children.the taxonomic position of a group of five d-sorbitol- and lactose-negative enterobacterial isolates recovered from diarrhoeal stools of children at the international centre for diarrhoeal disease research, bangladesh (icddr,b), was investigated by dna-dna hybridization, phenotypic characterization and 16s rdna sequencing. these strains were originally identified as 'hafnia alvei-like' with the api 20e system but, in fact, show more phenotypic and genotypic resemblance to members of the genus esc ...200312807204
[hafnia alvei bacteremia with liver and muscle abscess: a case report].hafnia alvei is a commensal organism of the digestive tract. it is best known as an agent of pediatric gastro-enteritis. in adults, this organism may exceptionally be responsible for extra-intestinal infections. in such cases, infections occur mainly in unusually susceptible patients, due to an underlying condition.200312876518
sanitary and bacteriological aspects of sewage treatment.a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ...200315058814
genetic and ecological structure of hafnia alvei in australia.multilocus enzyme electrophoresis of 161 hafnia alvei isolates from 158 hosts and 3 water column samples collected in australia revealed that this species consists of two genetically distinct groups. the two groups of h. alvei differed significantly in their genetic structure and host distribution. the taxonomic class of the host but not geographic locality explained a significant proportion of the observed genetic and biochemical variation among strains within each genetic group.200314666987
conjugative plasmid mediated inducible nickel resistance in hafnia alvei 5-5.hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree sebertia acuminata (sapotaceae) in new caledonia, was found to be resistant to 30 mm ni(2+) or 2 mm co(2+). the 70-kb plasmid, pejh 501, was transferred by conjugation to escherichia coli, serratia marcescens, and klebsiella oxytoca. transconjugant strains expressed inducible nickel resistance to between 5 and 17 mm ni(2+), and cobalt resistance to 2 mm co(2+). a 4.8-kb sal- ecori fra ...200312730713
nucleotide sequence and expression of the ncr nickel and cobalt resistance in hafnia alvei 5-5.the structural genes for the nickel and cobalt resistance of the conjugative plasmid pejh 501 of hafnia alvei 5-5, contained on a sali-ecori fragment of 4.8 kb, were cloned and sequenced. the dna sequence included five genes in the following order: ncra, ncrb, ncrc, ncry, and ncrx. the predicted amino acid sequences of ncra were homologous to the amino acid sequences of nreb of achromobacter xylosoxidans 31a. expression of ncr with the t7 rna polymerase-promoter system allowed escherichia coli b ...200415179604
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1529.the following structure of the pentasaccharide repeating unit of an acidic o-polysaccharide of hafnia alvei pcm 1529 was established by sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy: [carbohydrate structure: see text].200415013412
analysis of ampc beta-lactamase expression and sequence in biochemically atypical ceftazidime-resistant enterobacteriaceae from paediatric patients.to analyse the variation of ampc beta-lactamase gene sequence and expression in biochemically atypical enterobacteriaceae isolates, and to identify them definitively.200415014062
photobacterium phosphoreum caused a histamine fish poisoning incident.an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ...200415033270
evaluation of novel beta-ribosidase substrates for the differentiation of gram-negative bacteria.to synthesize novel substrates for the detection of beta-ribosidase and assess their potential for the differentiation of gram-negative bacteria.200414678171
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
the o-acetylation patterns in the o-antigens of hafnia alvei strains pcm 1200 and 1203, serologically closely related to pcm 1205.serological tests revealed immunochemical similarities between the lipopolysaccharides of hafnia alvei strains pcm 1200, 1203 and 1205. immunoblotting and elisa showed cross-reactions between the strains. nmr spectroscopy showed that the o-deacetylated o-specific polysaccharides isolated from lipopolysaccharides of h. alvei strains pcm 1200 and 1203 possessed the same composition and sequence as the o-deacetylated o-specific polysaccharide of h. alvei strain pcm 1205, that is a glycerol teichoic ...200415476713
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
chimeric nature of two plasmids of hafnia alvei encoding the bacteriocins alveicins a and b.the complete nucleotide sequences of two bacteriocin-encoding plasmids isolated from hafnia alvei (palva and palvb) were determined. both plasmids resemble cole1-type replicons and carry mobilization genes, as well as colicin-like bacteriocin operons. these bacteriocins appear to be chimeras consisting of translocation domains from tol-dependent colicins, unique binding domains, and killing and immunity domains similar to those of the pore-forming colicin ia. just as is found for colicin ia, the ...200414996789
late-onset neonatal sepsis due to hafnia alvei.hafnia alvei infections are uncommon, and usually occur in patients with underlying illnesses, mainly adults. the authors describe a rare case of late-onset community-acquired neonatal sepsis in an infant without any underlying disease. the infant was successfully treated with cefotaxime.200415000567
volatile compounds produced in cheese by enterobacteriaceae strains of dairy origin.the formation of volatile compounds in fresh cheese by 10 enterobacteriaceae strains of dairy origin (4 hafnia alvei, 2 serratia liquefaciens, 1 enterobacter cloacae, 1 enterobacter sakazakii, and 2 escherichia coli strains) was investigated. small cheeses were made from pasteurized cow's milk separately inoculated with 1-3 x 10(3) cfu/ml of each of the enterobacteriaceae strains, with glucono-8-lactone added to achieve a ph value of 5.2 in the curds. all strains reached counts close to 10(8) cf ...200415035375
dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates.enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ...200415046311
presence of acylated homoserine lactones (ahls) and ahl-producing bacteria in meat and potential role of ahl in spoilage of meat.quorum-sensing (qs) signals (n-acyl homoserine lactones [ahls]) were extracted and detected from five commercially produced vacuum-packed meat samples. ninety-six ahl-producing bacteria were isolated, and 92 were identified as enterobacteriaceae. hafnia alvei was the most commonly identified ahl-producing bacterium. thin-layer chromatographic profiles of supernatants from six h. alvei isolates and of extracts from spoiling meat revealed that the major ahl species had an r(f) value and shape simi ...200415240313
hafnia alvei infection in pullets in italy.this paper describes an outbreak of disease caused by hafnia alvei in pullets. cloudy swelling and the fatty degeneration of liver associated with splenic lymphocytic depletion were the most preminent lesions. the organism was identified by biochemical tests and by a hafnia-specific bacteriophage test. laying hens and pullets were infected experimentally with the organism by the oral and intraperitoneal route, and the clinical and pathological effects were similar to those observed in naturally ...200415276988
detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor.biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ...200415466560
detection of ticarcillin-clavulonic acid susceptibility with microdilution method in citrobacter, hafnia, proteus and some gram negative bacteria.the broth dilution method has been regarded as a good alternative test for detection of susceptibilities to antimicrobial agents. in this study, the antimicrobial activity of ticarcillin-clavulonic acid (tim) was investigated by the minimal inhibitory concentration (mic) method on strains of aeromonas, citrobacter, hafnia, morganella, proteus, pseudomonas and gram negative bacteria isolated from raw milk. the isolate collection included 91 gram negative strains. fifty-one (56.04%) isolates were ...200415508433
antimicrobial susceptibility patterns, beta-lactamases, and biochemical identification of yokenella regensburgei strains.yokenella regensburgei is an opportunistic human pathogen that phenotypically resembles hafnia alvei. the susceptibility of 10 y. regensburgei strains to 75 antimicrobial agents was examined, applying a microdilution procedure in cation-adjusted mueller-hinton broth (camhb) and isosensitest broth (isb). beta-lactamases were characterized phenotypically with beta-lactamase activity and induction assays. genotypically, pcr experiments applying degenerated primer pairs for the detection of ampc bet ...200414761716
[nosocomial hafnia alvei sepsis in a neonatal intensive care unit].hafnia alvei is a member of the enterobacteriaceae family, which is rarely associated with infection in pediatric patients and exceptionally in the neonatal period. infections caused by this organism are usually opportunistic. h. alvei bacteremias are mostly associated with abdominal disease or immunosuppression. we report four cases of nosocomial sepsis in preterm infants with positive blood cultures to h. alvei that were treated in our institution within a short period of time.200414987520
[histopathologic studies in experimental uveitis].experimental uveitis is one of the main models in the diseases of autoimmunological background. the purpose of this paper was to analyze the quantitative histological changes in the experimental uveitis, induced by different types of homogenous endotoxin salts of havnia alvei. we studied 74 eyes of lewis rats (males) divided into 4 groups. each group received a homogenous salt of havnia alvei in a single subcutaneous injection. in the 1 group-lps ca++, in 2 group-lps na++, in 3 group-lps 981, in ...200415636226
isolation and characterization of microorganisms from instruments used by pedicurists operating within lagos metropolis, nigeria.eight bacterial and five fungal species were isolated from swab samples taken from instruments used by pedicurists operating at three different sites in lagos, nigeria. the bacterial isolates included micrococcus luteus, micrococcus roseus, staphylococcus epidermidis, staphylococcus aureus, hafnia spp, shigella spp, bacillus subtilis and bacillus spp. the five fungal isolates were identified as aspergillus niger, aspergillus flavus, mucor spp, trichophyton spp and candida albicans. the presence ...200415816270
abdominal abscess and hafnia alvei septicemia occurring during the aplastic phase after autologous stem-cell transplantation in a patient with diffuse large b-cell lymphoma.hafnia alvei is a motile gram-negative bacterium that is rarely isolated from human specimens, but that sometimes can be found as part of the gastrointestinal flora. here we report a rare case of hafnia alvei septicemia with an abdominal abscess in a 60-year-old woman with diffuse large b-cell lymphoma involving the spleen, liver, and then lymph nodes. she initially received a splenectomy, and, over a 2-year period, four courses of chemotherapy. after achieving complete remission status, she und ...200416163467
clinical characteristics of nosocomial and community-acquired extraintestinal infections caused by hafnia alvei.36 episodes (25 nosocomial and 11 community-acquired) of infections caused by hafnia alvei showed that this bacterium is responsible for serious infections in adults, specially in hospitalized patients with underlying chronic diseases, subjected to invasive procedures or even under antibiotic treatment.200516308222
the effect of electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on raw chicken breasts stored at four degrees celsius for fourteen days.the effect of high-energy electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on commercial chicken breast meat was evaluated. fresh chicken breast meat was purchased from a local poultry processor, inoculated with 8 log10 cfu/ml salmonella, packaged in styrofoam trays and over wrapped with a polyvinyl chloride film, and subjected to 0, 1, 2, or 3 kgy of irradiation. the packaged samples were stored at 4 degrees c and analyzed for salm ...200515971537
raman and brillouin scattering spectroscopy studies of atomic layer-deposited zro(2) and hfo(2) thin films.atomic layer-deposited zro(2) (zirconia) and hfo(2) (hafnia) films with various thicknesses, ranging from 112 to 660 nm, have been studied by raman scattering spectroscopy. spectral analysis of the excellent quality raman data obtained by using freestanding edges of the films has unambiguously demonstrated that a metastable tetragonal t-zro(2) is coexisting with the stable monoclinic phase in zirconia films. even though the raman spectrum signal-to-noise ratio was high, only the monoclinic phase ...200516029867
[hafnia alvei in a rare case of severe pneumonia in a postanesthesia recovery unit].an 85-year-old man with a history of moderate chronic obstructive pulmonary disease in treatment with anticholinergic drugs was admitted to the recovery unit for postoperative monitoring after right inguinal herniorrhaphy surgery and removal of a strangulated intestinal section. on the third day after surgery the patient developed radiographic signs consistent with pneumonia and required mechanical ventilation. two blood cultures and a respiratory secretion sample grew a microorganism identified ...200516038176
isolation of vibrio cholerae from neonates admitted to an urban diarrhoeal diseases hospital in bangladesh.very little is known about vibrio cholerae infection in neonates with diarrhoea.200516156982
metal oxide monolithic columns.metal oxide monoliths composed of zro2 and hfo2 have been synthesized in situ inside capillary columns. the material shows globular-like structure and through pores. capillary electrochromatography and capillary liquid chromatography were performed in a monolithic column with the hfo2 material. separation of a simple sample mixture showed the potential of the new metal oxide monolithic columns.200516038327
natural antimicrobial susceptibility patterns and biochemical identification of escherichia albertii and hafnia alvei strains.bangladeshi diarrheagenic hafnia alvei-like strains have been described recently as the new species escherichia albertii (int j syst evolut microbiol. 2003;53:807-810). the natural susceptibility of 21 e. albertii and 76 h. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in isosensitest broth (for all the strains) and cation-adjusted mueller-hinton broth (for some strains). examining the phenotypic features of both taxa with commercial identification sys ...200515766600
evolutionary genetics of a new pathogenic escherichia species: escherichia albertii and related shigella boydii strains.a bacterium originally described as hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic escherichia coli. subsequent studies identified similar h. alvei-like strains that are positive for an intimin gene (eae) probe and, based on dna relatedness, are classified as a distinct escherichia species, escherichia albertii. we determined sequences for multiple housekeeping genes in five e. albertii strains and c ...200515629933
virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l.virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ...200516083446
detection of n-acyl-l-homoserine lactones degradation by bacillus spp. 200516366266
structure of the o-polysaccharide of hafnia alvei strain pcm 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation.the o-polysaccharide of hafnia alvei pcm 1189 consists of d-glucose, d-galactose, d-galnac and d-glca and lacks the strict regularity. the intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and nmr spectroscopy. the following structure was established for the o-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral ...200515639246
spontaneous bacterial peritonitis due to hafnia alvei in a patient with peritoneal mesothelioma. 200515756379
identification of two distinct hybridization groups in the genus hafnia by 16s rrna gene sequencing and phenotypic methods.a collection of 52 strains belonging to the hafnia alvei complex were subjected to molecular (16s rrna gene sequencing) and biochemical analysis. based upon 16s rrna gene sequencing results, two genetic groups were identified which correspond with previously recognized dna hybridization group 1 (atcc 13337(t) and atcc 29926; n = 23) and dna hybridization group 2 (atcc 29927; n = 29). of 46 biochemical tests used to characterize hafniae, 19 reactions (41%) yielded variable results. of these 19 te ...200516000455
structures of the biological repeating units in the o-chain polysaccharides of hafnia alvei strains having a typical lipopolysaccharide outer core region.earlier, the structures of the o-chain polysaccharides of the lipopolysaccharides (lps) of a number of hafnia alvei strains have been established. however, it remained unknown, which is the first and the last monosaccharide of the o-chain. this is defined by the structure of the so-called biological repeating unit (o-unit), which is pre-assembled and then polymerised in the course of biosynthesis of bacterial polysaccharides by the wzy-dependent pathway. now we report on the structures of the o- ...200515961298
genetic environment of acquired bla(acc-1) beta-lactamase gene in enterobacteriaceae isolates.we studied the genetic organization of bla(acc-1) in 14 isolates of enterobacteriaceae from france, tunisia, and germany. in a common ancestor, isecp1 was likely involved in the mobilization of this gene from the hafnia alvei chromosome to a plasmid. other genetic events involving insertion sequences (particularly is26), transposons (particularly tn1696), or suli-type integrons have occurred, leading to complex genetic environments.200616982793
misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system.nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ...200617050821
a complex of lactoferrin with monophosphoryl lipid a is an efficient adjuvant of the humoral and cellular immune response in mice.our recent investigations demonstrated adjuvant properties of lactoferrin (lf). other studies proved efficacy and safety of monophosphoryl lipid a (mpl) as an adjuvant in humans. in an attempt to construct more efficient and safer adjuvants, we evaluated the activity of lf-mpl complex, formed by incubation of lf and mpl from hafnia alvei at 20:1 w/w ratio, and verified its characteristics by sds-page analysis. binding kinetics was determined by surface plasmon resonance analysis using a biacore ...200616838169
structure and serological analysis of the hafnia alvei 481-l o-specific polysaccharide containing phosphate in the backbone chain.the lipopolysaccharide was extracted from cells of hafnia alvei 481-l bacterial strain and, after mild acid hydrolysis, the o-specific polysaccharide was isolated and characterised. on the basis of chemical analyses and nmr spectroscopic studies of the polysaccharide and oligosaccharides obtained after smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the o-specific polysaccharide is a phosphorylated hexasaccharide: [see text]. the biological repeating un ...200617081508
[beta-lactamases of gram negative bacteria: never-ending clockwork!].the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ...200616420989
isolation of toxigenic hafnia alvei from a probable case of hemolytic uremic syndrome.an 11-year-old girl presented to a central california children's hospital with a 3-day history of erythematous lesions on her forehead, neck, and trunk, abdominal pain, persistent emesis, and decreased urinary output. one day prior to admission she had a mild bout of diarrhea with a small amount of blood in her stool. upon admission her condition rapidly worsened with acute renal failure, anemia, and thrombocytopenia. one of the possible causes of this condition included hemolytic uremic syndrom ...200616896583
population-based laboratory surveillance of hafnia alvei isolates in a large canadian health region.hospital-based series have characterized hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness.200616707023
comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice.the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ...200616416921
etiologic agents of cervicovaginitis in turkish women.to investigate the distribution of microbiologic agents causing cervicovaginitis.200617013472
enterobacterial 38-kda outer membrane protein is an age-dependent molecular marker of innate immunity and immunoglobulin deficiency as results from its reactivity with igg and iga antibody.in earlier studies on an animal model we observed protective properties of outer membrane proteins (omps) of shigella, hafnia, and escherichia coli strains. in order to investigate human sera for reactivity with omps we subjected these proteins to immunoblotting with umbilical cord plasma and sera from children and adults. the igg and iga antibodies interacted primarily with a 38-kda protein, in similar way for several enterobacterial strains, but different for pseudomonas aeruginosa. this obser ...200617064276
characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1.to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples.200616430517
occurrence of proteolytic activity and n-acyl-homoserine lactone signals in the spoilage of aerobically chill-stored proteinaceous raw foods.proteolytic pseudomonads dominate the spoilage flora of aerobically chill-stored proteinaceous raw foods. proteolysis during spoilage of these food systems affects both food quality and the dynamics of the bacterial community because it increases the availability of nutrients to the community as a whole. quorum sensing, or cell-cell signaling, is associated closely with ecological interactions among bacteria in mixed communities. the potential role of quorum sensing in proteolytic food spoilage ...200617133818
the genus hafnia: from soup to nuts.the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ...200616418520
[obstructive pyocholecystitis and septicaemia related from hafnia alvei: influence of diabetes mellitus]. 200616716560
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
[bacteremia caused by hafnia alvei in an intensive care neonatal unit]. 200616650371
young-infant sepsis combined with urinary tract infection due to hafnia alvei.hafnia alvei infections are uncommon and occur mainly in adult patients featuring underlying illnesses. its isolation in pediatric cases is even more unusual. we report a rare case of sepsis combined with urinary tract infection caused by h. alvei in a 39-day-old infant who did not appear to feature any underlying disease. the infant was successfully treated with ceftriaxone over a 14-day period. in this case, we want to remind clinicians that the possibility of an extraintestinal invasive infec ...200717493908
microbiological characteristics of "androlla", a spanish traditional pork sausage.counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ...200716943094
increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability.there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ...200717007934
failure to detect salmonella species in a population of wild tuatara (sphenodon punctatus).to assess the prevalence of faecal excretion of salmonella serovars by wild tuatara (sphenodon punctatus) on stephens island, new zealand.200717534416
[genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal diseases].in the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from enterobacteriaceae family 89 microorganisms were isolated including klebsiella spp. (37 isolates), citrobacter spp. (9 isolates), enterobacter spp. (17 isolates), hafnia alvei (1 isolate), morganella morganii (11 isolates), proteus spp. (14 isolates). presence of genes associated with pathogenicity islands (pais): hlya, hlyb (hemolysin), sfag (fimbria antigen type s), cnf1 (cytot ...200717523471
hafnia alvei endocarditis following pyelonephritis in a permanent pacemaker carrier. 200717256223
possible involvement of psychrotolerant enterobacteriaceae in blown pack spoilage of vacuum-packaged raw meats.recent investigations of blown pack spoilage in new zealand chilled vacuum-packaged meats have found moderate to high numbers of enterobacteriaceae in the spoilage flora, but no clostridia, such as c. estertheticum and c. gasigenes, that are usually associated with blown pack spoilage. this study showed that pyschrotolerant enterobacteriaceae produced gas in a lamb homogenate model under anaerobic conditions and that these organisms could cause blown pack spoilage of vacuum-packaged chilled meat ...200717870199
contamination of milk by enterococci and coliforms from bovine faeces.to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ...200717953550
antibiotic resistance and adherence properties of hafnia alvei clinical isolates: a 19-month study in the hospital of orléans, france.we studied the epidemiology of antibiotic resistance and adherence properties among all hafnia alvei clinical isolates collected from august 2003 to february 2005 from patients hospitalized in the hospital of orléans, france. the isolates were typed by random amplified polymorphic dna (rapd), screened for antibiotic resistance and bacterial adherence to a549 respiratory and t24 bladder cells. six intestinal, 3 respiratory, and 8 isolates from different body sites were collected. a total of 12 ra ...200718230550
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