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late-onset neonatal sepsis due to hafnia alvei.hafnia alvei infections are uncommon, and usually occur in patients with underlying illnesses, mainly adults. the authors describe a rare case of late-onset community-acquired neonatal sepsis in an infant without any underlying disease. the infant was successfully treated with cefotaxime.200415000567
studies on the hafnia isolated from normal human. 196314099091
analysis of ampc beta-lactamase expression and sequence in biochemically atypical ceftazidime-resistant enterobacteriaceae from paediatric patients.to analyse the variation of ampc beta-lactamase gene sequence and expression in biochemically atypical enterobacteriaceae isolates, and to identify them definitively.200415014062
photobacterium phosphoreum caused a histamine fish poisoning incident.an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ...200415033270
a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe. 199811864254
ion-assisted deposition of moisture-stable hafnium oxide films for ultraviolet applications.a design-of-experiments statistical approach was taken to determine the optimum ion gun operating parameters for the deposition of moisture-stable, low-absorbing hafnium oxide films by ion-assisted electron-beam evaporation. factors identified as affecting the quality of hafnia films were chamber pressure, deposition rate, ion gun source gas composition, and ion gun current. both oxygen and argon were used as source gases. high and low levels of the factors were chosen on the basis of our experi ...200212064403
[research on a severe hafnia epizootic in young laboratory rats]. 196314076976
sanitary and bacteriological aspects of sewage treatment.a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ...200315058814
a molecular phylogeny of enteric bacteria and implications for a bacterial species concept.a molecular phylogeny for seven taxa of enteric bacteria (citrobacter freundii, enterobacter cloacae, escherichia coli, hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, and serratia plymuthica) was made from multiple isolates per taxa taken from a collection of environmental enteric bacteria. sequences from five housekeeping genes (gapa, groel, gyra, ompa, and pgi) and the 16s rrna gene were used to infer individual gene trees and were concatenated to infer a composite molecular phylogen ...200314640415
presence of acylated homoserine lactones (ahls) and ahl-producing bacteria in meat and potential role of ahl in spoilage of meat.quorum-sensing (qs) signals (n-acyl homoserine lactones [ahls]) were extracted and detected from five commercially produced vacuum-packed meat samples. ninety-six ahl-producing bacteria were isolated, and 92 were identified as enterobacteriaceae. hafnia alvei was the most commonly identified ahl-producing bacterium. thin-layer chromatographic profiles of supernatants from six h. alvei isolates and of extracts from spoiling meat revealed that the major ahl species had an r(f) value and shape simi ...200415240313
[bilateral lung tuberculosis and over-infection by hafnia alvei]. 200212481504
characterisation of hafnia alvei isolates from human clinical extra-intestinal specimens: haemagglutinins, serum resistance and siderophore synthesis.extra-intestinal hafnia alvei isolates are rarely considered to be pathogenic. to investigate whether such strains are able to produce virulence factors, a total of 70 clinical h. alvei isolates was compared with clinical extra-intestinal isolates of other members of the enterobacterial tribe klebsiellae (kiebsiella pneumoniae, enterobacter cloacae, serratia marcescens). whereas mannose-sensitive haemagglutination (msha) was less common in h. alvei (59%) than in k. pneumoniae (86%) and e. cloaca ...200111232764
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
theoretical evaluation of zirconia and hafnia as gate oxides for si microelectronics.parameters determining the performance of the crystalline oxides zirconia (zro2) and hafnia (hfo2) as gate insulators in nanometric si electronics are estimated via ab initio calculations of the energetics, dielectric properties, and band alignment of bulk and thin-film oxides on si (001). with their large dielectric constants, stable and low-formation-energy interfaces, large valence offsets, and reasonable (though not optimal) conduction offsets (electron injection barriers), zirconia and hafn ...200212484834
detection of ticarcillin-clavulonic acid susceptibility with microdilution method in citrobacter, hafnia, proteus and some gram negative bacteria.the broth dilution method has been regarded as a good alternative test for detection of susceptibilities to antimicrobial agents. in this study, the antimicrobial activity of ticarcillin-clavulonic acid (tim) was investigated by the minimal inhibitory concentration (mic) method on strains of aeromonas, citrobacter, hafnia, morganella, proteus, pseudomonas and gram negative bacteria isolated from raw milk. the isolate collection included 91 gram negative strains. fifty-one (56.04%) isolates were ...200415508433
mechanism of interstitial oxygen diffusion in hafnia.we have performed density functional calculations of oxygen incorporation and diffusion in monoclinic hafnia (hfo2) for a range of oxygen charge states. the calculations demonstrate that oxygen favors atomic incorporation and that o2- is the most stable species. we find that oxygen interstitials diffuse via exchange with lattice oxygen sites in hafnia, and that o- species have the smallest diffusion barrier.200212485083
hafnia alvei strains possessing the alpha antigen of stamp and stone. 196113696727
conjugative plasmid mediated inducible nickel resistance in hafnia alvei 5-5.hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree sebertia acuminata (sapotaceae) in new caledonia, was found to be resistant to 30 mm ni(2+) or 2 mm co(2+). the 70-kb plasmid, pejh 501, was transferred by conjugation to escherichia coli, serratia marcescens, and klebsiella oxytoca. transconjugant strains expressed inducible nickel resistance to between 5 and 17 mm ni(2+), and cobalt resistance to 2 mm co(2+). a 4.8-kb sal- ecori fra ...200312730713
escherichia albertii sp. nov., a diarrhoeagenic species isolated from stool specimens of bangladeshi children.the taxonomic position of a group of five d-sorbitol- and lactose-negative enterobacterial isolates recovered from diarrhoeal stools of children at the international centre for diarrhoeal disease research, bangladesh (icddr,b), was investigated by dna-dna hybridization, phenotypic characterization and 16s rdna sequencing. these strains were originally identified as 'hafnia alvei-like' with the api 20e system but, in fact, show more phenotypic and genotypic resemblance to members of the genus esc ...200312807204
the structure of the o-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of hafnia alvei that has characteristics of a new species under the genus escherichia.the structure of the o-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in bangladesh was studied with sugar, and methylation analysis, nmr spectroscopy, mass spectrometry and partial acid hydrolysis. the strain was first designated as hafnia alvei, but later found to be a possible new species in the genus escherichia. two different polysaccharides were detected, a major and a minor one. the structure of the major polysaccharide is given below, while the structure of the ...200212084070
evaluation of increased incubation temperature and cefixime-tellurite treatment for the isolation of escherichia coli o157:h7 from minced beef.to improve enrichment and isolation of escherichia coli o157:h7, this study evaluated increased incubation temperature and cefixime-tellurite (ct) on five strains of each of the following bacteria, e. coli, hafnia alvei, enterobacter spp., citrobacter freundii and e. coli o157:h7, and two strains of e. coli o157:nh7. these were grown in pure culture in lst broth with varying cefixime-tellurite concentrations. a range of incubation temperatures from 37 to 46 degrees c was investigated for the inh ...200312927704
[hafnia alvei septicemia revealing pyocholecystitis complicated by liver abscess in an immunocompetent patient]. 200011094615
adansonian analysis and deoxyribonucleic acid base composition of serratia marcescens.colwell, r. r. (georgetown university, washington, d.c.), and m. mandel. adansonian analysis and deoxyribonucleic acid base composition of serratia marcescens. j. bacteriol. 89:454-461. 1965.-a total of 33 strains of serratia marcescens were subjected to adansonian analysis for which more than 200 coded features for each of the organisms were included. in addition, the base composition [expressed as moles per cent guanine + cytosine (g + c)] of the deoxyribonucleic acid (dna) prepared from each ...196514255714
studies on the hafnia group of enterobacteriaceae. 196114496196
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1546.an acidic o-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of hafnia alvei pcm 1546 is composed of d-gal, d-glc, d-glca, d-galnac and o-acetyl groups in the ratios 1:1:1:2:1.6. on the basis of sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [see equation in text].200314505884
detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor.biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ...200415466560
dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates.enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ...200415046311
biochemical properties of a newly described escherichia species, escherichia albertii.five strains of a newly described escherichia species, escherichia albertii, were extensively characterized by conventional biochemical methods and by commercial identification panels. e. albertii is an indole-negative species that ferments d-mannitol but not d-xylose. because these strains are not included in the databases of commercial systems at present, they were most often identified as hafnia, salmonella, escherichia coli, or, on one system (microscan dried overnight panels), yersinia ruck ...200314532241
nucleotide sequence and expression of the ncr nickel and cobalt resistance in hafnia alvei 5-5.the structural genes for the nickel and cobalt resistance of the conjugative plasmid pejh 501 of hafnia alvei 5-5, contained on a sali-ecori fragment of 4.8 kb, were cloned and sequenced. the dna sequence included five genes in the following order: ncra, ncrb, ncrc, ncry, and ncrx. the predicted amino acid sequences of ncra were homologous to the amino acid sequences of nreb of achromobacter xylosoxidans 31a. expression of ncr with the t7 rna polymerase-promoter system allowed escherichia coli b ...200415179604
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
[nosocomial pneumonia caused by hafnia alvei]. 200314624659
[enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital].to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms.200211939120
hafnia alvei infection in pullets in italy.this paper describes an outbreak of disease caused by hafnia alvei in pullets. cloudy swelling and the fatty degeneration of liver associated with splenic lymphocytic depletion were the most preminent lesions. the organism was identified by biochemical tests and by a hafnia-specific bacteriophage test. laying hens and pullets were infected experimentally with the organism by the oral and intraperitoneal route, and the clinical and pathological effects were similar to those observed in naturally ...200415276988
[hafnia]. 196414156599
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
caseinolysis in cheese by enterobacteriaceae strains of dairy origin.to study the effect of enterobacteriaceae strains of dairy origin on caseins under cheese manufacture and ripening conditions.200314633113
[beta-lactamases of gram negative bacteria: never-ending clockwork!].the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ...200616420989
evolutionary genetics of a new pathogenic escherichia species: escherichia albertii and related shigella boydii strains.a bacterium originally described as hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic escherichia coli. subsequent studies identified similar h. alvei-like strains that are positive for an intimin gene (eae) probe and, based on dna relatedness, are classified as a distinct escherichia species, escherichia albertii. we determined sequences for multiple housekeeping genes in five e. albertii strains and c ...200515629933
[histopathologic studies in experimental uveitis].experimental uveitis is one of the main models in the diseases of autoimmunological background. the purpose of this paper was to analyze the quantitative histological changes in the experimental uveitis, induced by different types of homogenous endotoxin salts of havnia alvei. we studied 74 eyes of lewis rats (males) divided into 4 groups. each group received a homogenous salt of havnia alvei in a single subcutaneous injection. in the 1 group-lps ca++, in 2 group-lps na++, in 3 group-lps 981, in ...200415636226
[bacteremia caused by hafnia alvei in an intensive care neonatal unit]. 200616650371
genetic and ecological structure of hafnia alvei in australia.multilocus enzyme electrophoresis of 161 hafnia alvei isolates from 158 hosts and 3 water column samples collected in australia revealed that this species consists of two genetically distinct groups. the two groups of h. alvei differed significantly in their genetic structure and host distribution. the taxonomic class of the host but not geographic locality explained a significant proportion of the observed genetic and biochemical variation among strains within each genetic group.200314666987
the eaea gene is not found in hafnia alvei from patients with diarrhea in aragón, spain.a total of 102 hafnia alvei clinical strains isolated from different patients with diarrhea has been tested, using polymerase chain reaction and dot-blot hybridization, for the enteropathogenic escherichia coli attaching and effacing a (eaea) gene to establish their role as a causative agent of diarrhea in our environment. none of them was positive for the eaea gene. we cannot consider the eaea gene as the virulence-associated factor implicated in the h. alvei strains isolated from diarrheal fec ...200111770828
electron energy-loss spectroscopy study of thin film hafnium aluminates for novel gate dielectrics.we have used conventional high-resolution transmission electron microscopy and electron energy-loss spectroscopy (eels) in scanning transmission electron microscopy to investigate the microstructure and electronic structure of hafnia-based thin films doped with small amounts (6.8 at.%) of al grown on (001) si. the as-deposited film is amorphous with a very thin (approximately 0.5 nm) interfacial siox layer. the film partially crystallizes after annealing at 700 degrees c and the interfacial sio2 ...200312694419
isolation and characterization of microorganisms from instruments used by pedicurists operating within lagos metropolis, nigeria.eight bacterial and five fungal species were isolated from swab samples taken from instruments used by pedicurists operating at three different sites in lagos, nigeria. the bacterial isolates included micrococcus luteus, micrococcus roseus, staphylococcus epidermidis, staphylococcus aureus, hafnia spp, shigella spp, bacillus subtilis and bacillus spp. the five fungal isolates were identified as aspergillus niger, aspergillus flavus, mucor spp, trichophyton spp and candida albicans. the presence ...200415816270
[nosocomial hafnia alvei sepsis in a neonatal intensive care unit].hafnia alvei is a member of the enterobacteriaceae family, which is rarely associated with infection in pediatric patients and exceptionally in the neonatal period. infections caused by this organism are usually opportunistic. h. alvei bacteremias are mostly associated with abdominal disease or immunosuppression. we report four cases of nosocomial sepsis in preterm infants with positive blood cultures to h. alvei that were treated in our institution within a short period of time.200414987520
additional new antigens of hafnia group. 196414301054
increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability.there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ...200717007934
volatile compounds produced in cheese by enterobacteriaceae strains of dairy origin.the formation of volatile compounds in fresh cheese by 10 enterobacteriaceae strains of dairy origin (4 hafnia alvei, 2 serratia liquefaciens, 1 enterobacter cloacae, 1 enterobacter sakazakii, and 2 escherichia coli strains) was investigated. small cheeses were made from pasteurized cow's milk separately inoculated with 1-3 x 10(3) cfu/ml of each of the enterobacteriaceae strains, with glucono-8-lactone added to achieve a ph value of 5.2 in the curds. all strains reached counts close to 10(8) cf ...200415035375
the genus hafnia: from soup to nuts.the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ...200616418520
enterobacterial 38-kda outer membrane protein is an age-dependent molecular marker of innate immunity and immunoglobulin deficiency as results from its reactivity with igg and iga antibody.in earlier studies on an animal model we observed protective properties of outer membrane proteins (omps) of shigella, hafnia, and escherichia coli strains. in order to investigate human sera for reactivity with omps we subjected these proteins to immunoblotting with umbilical cord plasma and sera from children and adults. the igg and iga antibodies interacted primarily with a 38-kda protein, in similar way for several enterobacterial strains, but different for pseudomonas aeruginosa. this obser ...200617064276
antimicrobial susceptibility patterns, beta-lactamases, and biochemical identification of yokenella regensburgei strains.yokenella regensburgei is an opportunistic human pathogen that phenotypically resembles hafnia alvei. the susceptibility of 10 y. regensburgei strains to 75 antimicrobial agents was examined, applying a microdilution procedure in cation-adjusted mueller-hinton broth (camhb) and isosensitest broth (isb). beta-lactamases were characterized phenotypically with beta-lactamase activity and induction assays. genotypically, pcr experiments applying degenerated primer pairs for the detection of ampc bet ...200414761716
the effect of electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on raw chicken breasts stored at four degrees celsius for fourteen days.the effect of high-energy electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on commercial chicken breast meat was evaluated. fresh chicken breast meat was purchased from a local poultry processor, inoculated with 8 log10 cfu/ml salmonella, packaged in styrofoam trays and over wrapped with a polyvinyl chloride film, and subjected to 0, 1, 2, or 3 kgy of irradiation. the packaged samples were stored at 4 degrees c and analyzed for salm ...200515971537
raman and brillouin scattering spectroscopy studies of atomic layer-deposited zro(2) and hfo(2) thin films.atomic layer-deposited zro(2) (zirconia) and hfo(2) (hafnia) films with various thicknesses, ranging from 112 to 660 nm, have been studied by raman scattering spectroscopy. spectral analysis of the excellent quality raman data obtained by using freestanding edges of the films has unambiguously demonstrated that a metastable tetragonal t-zro(2) is coexisting with the stable monoclinic phase in zirconia films. even though the raman spectrum signal-to-noise ratio was high, only the monoclinic phase ...200516029867
identification of two distinct hybridization groups in the genus hafnia by 16s rrna gene sequencing and phenotypic methods.a collection of 52 strains belonging to the hafnia alvei complex were subjected to molecular (16s rrna gene sequencing) and biochemical analysis. based upon 16s rrna gene sequencing results, two genetic groups were identified which correspond with previously recognized dna hybridization group 1 (atcc 13337(t) and atcc 29926; n = 23) and dna hybridization group 2 (atcc 29927; n = 29). of 46 biochemical tests used to characterize hafniae, 19 reactions (41%) yielded variable results. of these 19 te ...200516000455
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
natural antimicrobial susceptibility patterns and biochemical identification of escherichia albertii and hafnia alvei strains.bangladeshi diarrheagenic hafnia alvei-like strains have been described recently as the new species escherichia albertii (int j syst evolut microbiol. 2003;53:807-810). the natural susceptibility of 21 e. albertii and 76 h. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in isosensitest broth (for all the strains) and cation-adjusted mueller-hinton broth (for some strains). examining the phenotypic features of both taxa with commercial identification sys ...200515766600
virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l.virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ...200516083446
biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts.we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ...199416349260
detection of n-acyl-l-homoserine lactones degradation by bacillus spp. 200516366266
the o-acetylation patterns in the o-antigens of hafnia alvei strains pcm 1200 and 1203, serologically closely related to pcm 1205.serological tests revealed immunochemical similarities between the lipopolysaccharides of hafnia alvei strains pcm 1200, 1203 and 1205. immunoblotting and elisa showed cross-reactions between the strains. nmr spectroscopy showed that the o-deacetylated o-specific polysaccharides isolated from lipopolysaccharides of h. alvei strains pcm 1200 and 1203 possessed the same composition and sequence as the o-deacetylated o-specific polysaccharide of h. alvei strain pcm 1205, that is a glycerol teichoic ...200415476713
[genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal diseases].in the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from enterobacteriaceae family 89 microorganisms were isolated including klebsiella spp. (37 isolates), citrobacter spp. (9 isolates), enterobacter spp. (17 isolates), hafnia alvei (1 isolate), morganella morganii (11 isolates), proteus spp. (14 isolates). presence of genes associated with pathogenicity islands (pais): hlya, hlyb (hemolysin), sfag (fimbria antigen type s), cnf1 (cytot ...200717523471
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...199516535048
metal oxide monolithic columns.metal oxide monoliths composed of zro2 and hfo2 have been synthesized in situ inside capillary columns. the material shows globular-like structure and through pores. capillary electrochromatography and capillary liquid chromatography were performed in a monolithic column with the hfo2 material. separation of a simple sample mixture showed the potential of the new metal oxide monolithic columns.200516038327
isolation of vibrio cholerae from neonates admitted to an urban diarrhoeal diseases hospital in bangladesh.very little is known about vibrio cholerae infection in neonates with diarrhoea.200516156982
[hafnia alvei in a rare case of severe pneumonia in a postanesthesia recovery unit].an 85-year-old man with a history of moderate chronic obstructive pulmonary disease in treatment with anticholinergic drugs was admitted to the recovery unit for postoperative monitoring after right inguinal herniorrhaphy surgery and removal of a strangulated intestinal section. on the third day after surgery the patient developed radiographic signs consistent with pneumonia and required mechanical ventilation. two blood cultures and a respiratory secretion sample grew a microorganism identified ...200516038176
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1529.the following structure of the pentasaccharide repeating unit of an acidic o-polysaccharide of hafnia alvei pcm 1529 was established by sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy: [carbohydrate structure: see text].200415013412
comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice.the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ...200616416921
abdominal abscess and hafnia alvei septicemia occurring during the aplastic phase after autologous stem-cell transplantation in a patient with diffuse large b-cell lymphoma.hafnia alvei is a motile gram-negative bacterium that is rarely isolated from human specimens, but that sometimes can be found as part of the gastrointestinal flora. here we report a rare case of hafnia alvei septicemia with an abdominal abscess in a 60-year-old woman with diffuse large b-cell lymphoma involving the spleen, liver, and then lymph nodes. she initially received a splenectomy, and, over a 2-year period, four courses of chemotherapy. after achieving complete remission status, she und ...200416163467
the bryophyte genus sphagnum is a reservoir for powerful and extraordinary antagonists and potentially facultative human pathogens.sphagnum plants grow in natural, species-poor carpets at low ph but without any known substantial fungal disease. to investigate this phenomenon, we analysed bacterial populations associated with two sphagnum species with different ecological behaviour, namely s. magellanicum and s. fallax, from three sites in germany and three in norway, with a special focus on the functional group of antagonists. the screening of 493 bacterial isolates for antagonistic activity against fungal pathogens resulte ...200717484734
[pathogenicity factors of opportunistic enterobacteria and its role in development of diarrhea].the data of pathogenicity factors of opportunistic enterobacteria, including klebsiella, enterobacter, citrobacter, proteus, providencia and hafnia species are submitted. the genetic control and a role of pathogenicity factors of opportunistic enterobacteria in development of diarrhea syndrome are presented. data about adhesins, hemolysins, cytotoxic necrotizing factors and bacterial modulins are described. the characteristic of cytotonic and cytotoxic enterotoxins, including lt, st, shiga-like ...200717523440
clinical characteristics of nosocomial and community-acquired extraintestinal infections caused by hafnia alvei.36 episodes (25 nosocomial and 11 community-acquired) of infections caused by hafnia alvei showed that this bacterium is responsible for serious infections in adults, specially in hospitalized patients with underlying chronic diseases, subjected to invasive procedures or even under antibiotic treatment.200516308222
[hafnia alvei bacteremia with liver and muscle abscess: a case report].hafnia alvei is a commensal organism of the digestive tract. it is best known as an agent of pediatric gastro-enteritis. in adults, this organism may exceptionally be responsible for extra-intestinal infections. in such cases, infections occur mainly in unusually susceptible patients, due to an underlying condition.200312876518
failure to detect salmonella species in a population of wild tuatara (sphenodon punctatus).to assess the prevalence of faecal excretion of salmonella serovars by wild tuatara (sphenodon punctatus) on stephens island, new zealand.200717534416
absidia corymbifera in an immune competent accident victim with multiple abdominal injuries: case report.we report a case of mucormycosis in a healthy 17-year-old accident victim with multiple abdominal injuries which was caused by infection with absidia corymbifera, a ubiquitous saphrophyte in the ground.200717531089
characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1.to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples.200616430517
[obstructive pyocholecystitis and septicaemia related from hafnia alvei: influence of diabetes mellitus]. 200616716560
evidence of temporal postdischarge decontamination of bacteria by gliding electric discharges: application to hafnia alvei.this study aimed to characterize the bacterium-destroying properties of a gliding arc plasma device during electric discharges and also under temporal postdischarge conditions (i.e., when the discharge was switched off). this phenomenon was reported for the first time in the literature in the case of the plasma destruction of microorganisms. when cells of a model bacterium, hafnia alvei, were exposed to electric discharges, followed or not followed by temporal postdischarges, the survival curves ...200717557841
the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression.there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ...200818283240
isolation of toxigenic hafnia alvei from a probable case of hemolytic uremic syndrome.an 11-year-old girl presented to a central california children's hospital with a 3-day history of erythematous lesions on her forehead, neck, and trunk, abdominal pain, persistent emesis, and decreased urinary output. one day prior to admission she had a mild bout of diarrhea with a small amount of blood in her stool. upon admission her condition rapidly worsened with acute renal failure, anemia, and thrombocytopenia. one of the possible causes of this condition included hemolytic uremic syndrom ...200616896583
microbiological characteristics of "androlla", a spanish traditional pork sausage.counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ...200716943094
genetic environment of acquired bla(acc-1) beta-lactamase gene in enterobacteriaceae isolates.we studied the genetic organization of bla(acc-1) in 14 isolates of enterobacteriaceae from france, tunisia, and germany. in a common ancestor, isecp1 was likely involved in the mobilization of this gene from the hafnia alvei chromosome to a plasmid. other genetic events involving insertion sequences (particularly is26), transposons (particularly tn1696), or suli-type integrons have occurred, leading to complex genetic environments.200616982793
spontaneous bacterial peritonitis due to hafnia alvei in a patient with peritoneal mesothelioma. 200515756379
misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system.nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ...200617050821
structure and serological analysis of the hafnia alvei 481-l o-specific polysaccharide containing phosphate in the backbone chain.the lipopolysaccharide was extracted from cells of hafnia alvei 481-l bacterial strain and, after mild acid hydrolysis, the o-specific polysaccharide was isolated and characterised. on the basis of chemical analyses and nmr spectroscopic studies of the polysaccharide and oligosaccharides obtained after smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the o-specific polysaccharide is a phosphorylated hexasaccharide: [see text]. the biological repeating un ...200617081508
a complex of lactoferrin with monophosphoryl lipid a is an efficient adjuvant of the humoral and cellular immune response in mice.our recent investigations demonstrated adjuvant properties of lactoferrin (lf). other studies proved efficacy and safety of monophosphoryl lipid a (mpl) as an adjuvant in humans. in an attempt to construct more efficient and safer adjuvants, we evaluated the activity of lf-mpl complex, formed by incubation of lf and mpl from hafnia alvei at 20:1 w/w ratio, and verified its characteristics by sds-page analysis. binding kinetics was determined by surface plasmon resonance analysis using a biacore ...200616838169
examination of the new alpha-(2'z-fluoro)vinyl trigger with lysine decarboxylase: the absolute stereochemistry dictates the reaction course. 200717212389
population-based laboratory surveillance of hafnia alvei isolates in a large canadian health region.hospital-based series have characterized hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness.200616707023
anomalous behavior of the dielectric constant of hafnium silicates: a first principles study.we present an extensive ab initio study of the structural and dielectric properties of hafnium silicates hf(x)si(1-x)o(2) that accounts for the observed anomalous dependence on composition of the static dielectric constant in the entire x range. the results reveal that this complex behavior reflects that of the structural development with x, from silica to hafnia, and clarify how different growth processes can also lead to scattered sets of data. several simple models proposed thus far to explai ...200717358729
etiologic agents of cervicovaginitis in turkish women.to investigate the distribution of microbiologic agents causing cervicovaginitis.200617013472
first total synthesis of a pentasaccharide repeating unit of the o-antigen of hafnia alvei pcm 1529.a pentasaccharide repeating unit of the o-antigen of hafnia alvei has been synthesized in a concise manner. high yielding glycosylation steps and minimum number of protecting group manipulation steps are the key features of this synthesis. a two-step, one-pot phase transfer oxidation protocol has been applied for the preparation of d-galacturonic acid.200817659441
calorimetric measurements of energetics of defect interactions in fluorite oxides.direct measurement by oxide melt solution calorimetry of energetics of mixing in rare earth and yttrium doped zirconia, hafnia, and ceria systems provides support for spectroscopic and computational studies of the location and clustering of vacancies in these systems. strongly negative heats of mixing are seen when the vacancy is transferred from being nearest neighbor to y or re in the sesquioxide to being nearest neighbor to zr or hf in the cubic solid solution. in the absence of such redistri ...200717326568
young-infant sepsis combined with urinary tract infection due to hafnia alvei.hafnia alvei infections are uncommon and occur mainly in adult patients featuring underlying illnesses. its isolation in pediatric cases is even more unusual. we report a rare case of sepsis combined with urinary tract infection caused by h. alvei in a 39-day-old infant who did not appear to feature any underlying disease. the infant was successfully treated with ceftriaxone over a 14-day period. in this case, we want to remind clinicians that the possibility of an extraintestinal invasive infec ...200717493908
possible involvement of psychrotolerant enterobacteriaceae in blown pack spoilage of vacuum-packaged raw meats.recent investigations of blown pack spoilage in new zealand chilled vacuum-packaged meats have found moderate to high numbers of enterobacteriaceae in the spoilage flora, but no clostridia, such as c. estertheticum and c. gasigenes, that are usually associated with blown pack spoilage. this study showed that pyschrotolerant enterobacteriaceae produced gas in a lamb homogenate model under anaerobic conditions and that these organisms could cause blown pack spoilage of vacuum-packaged chilled meat ...200717870199
ultrathin oxides: bulk-oxide-like model surfaces or unique films?to better understand the electronic and chemical properties of wide-gap oxide surfaces at the atomic scale, experimental work has focused on epitaxial films on metal substrates. recent findings show that these films are considerably thinner than previously thought. this raises doubts about the transferability of the results to surface properties of thicker films and bulk crystals. by means of density-functional theory and approximate gw corrections for the electronic spectra we demonstrate for t ...200717930957
contamination of milk by enterococci and coliforms from bovine faeces.to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ...200717953550
microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457.concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457 is reported. construction of the tetrasaccharide as its 4-methoxyphenyl glycoside was achieved by condensation of less abundant monosaccharide units such as, d-galactofuranose, n-acetyl-d-galactosamine and n-acetylneuraminic acid. the synthetic strategy consists of the preparation of suitably protected required monosaccharide intermediates from the commercially available reducing sugars and high ...200818074224
influence of environmental conditions on biofilm formation by hafnia alvei strains.hafnia alvei is considered an opportunistic pathogen of animal and humans, affecting a wide range of homeothermic and poikilothermic hosts with different body temperatures. in this work, h. alvei strains isolated from different sources were studied with regard to their capacity to form biofilms under different environmental conditions. strain, growth phase, temperature and culture media dependent changes of biofilm formation were semiquantitatively monitored using a microtiter plate method. our ...200818093756
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
structure of the o-polysaccharide of hafnia alvei strain pcm 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation.the o-polysaccharide of hafnia alvei pcm 1189 consists of d-glucose, d-galactose, d-galnac and d-glca and lacks the strict regularity. the intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and nmr spectroscopy. the following structure was established for the o-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral ...200515639246
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