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the application of 1h/13c inversely correlated nmr spectroscopy to the determination of acylation and glycosylation sites in the o-specific polysaccharide from hafnia alvei 1187.an inversely correlated 1h/13c nmr spectrum defined the amino sugars acylated by acetyl or 3-hydroxybutyryl groups and revealed partial sequences and glycosylation sites in a tetrasaccharide repeating unit of the title polysaccharide, (----2dglc alpha 1----3dglcnacyl alpha 1----4dgalnac alpha 1----3dgalnac beta 1----)n, where acyl = 3-hydroxybutyryl.19873619933
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
a study of predominant aerobic microflora of black bears (ursus americanus) and grizzly bears (ursus arctos) in northwestern alberta.swab specimens were obtained from nasal, rectal, and preputial or vaginal areas of 37 grizzly and 17 black bears, captured during may to june of 1981 to 1983, to determine the types and frequency of predominant aerobic microflora. bacterial genera most frequently isolated from bears were escherichia, citrobacter, hafnia, proteus, staphylococcus, and streptococcus species, comprising about 65% of the isolates. erwinia, xanthomonas, agrobacterium, rhizobium, and gluconobacter/acetobacter were also ...19873447691
[comparative activity of habekacin and 4 other aminoglycosides against gram-negative bacilli. evolution of resistance].the activity of 5 aminoglycosides compounds (habekacin, amikacin, gentamicin, netilmicin and tobramycin) was studied by an agar dilution method, against 235 strains of enterobacteriaceae and 146 other gram negative bacilli. 79 to 98% of susceptible strains were observed, according to the aminoglycoside compound. habekacin and amikacin were the most effective, specially against the more frequently resistant bacteria: enterobacter, serratia, hafnia, acinetobacter, pseudomonas aeruginosa. in compar ...19883136424
further differentiation of enterobacteriaceae by means of siderophore-pattern analysis.by means of a combination of 5 siderophore bioassays using several indicator strains, genera, species and subspecies of enterobacteriaceae can be further differentiated. enterobactin, aerobactin and other siderophores produced can be detected. each strain shows specific pattern which we called siderophore-pattern. it is easy to separate morganella, proteus, providencia, yersinia strains from the genera salmonella, shigella, escherichia coli, enterobacter, citrobacter, klebsiella, serratia. enter ...19882970195
hafnia alvei septicemia in an infant with necrotizing enterocolitis.hafnia alvei is an infrequently reported pathogen in children, and its isolation in a newborn is even more unusual. this organism is rarely associated with invasive disease. this article reports the first case of a neonate with necrotizing enterocolitis and subsequent ileal perforation who had h. alvei isolated from both blood and stool.19883057140
[biochemical typing of enterobacter isolated from several clinical materials].a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ...19883078792
[the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar].from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified.19883240566
outer membrane protein composition of hafnia alvei.the outer membrane proteins (omp) obtained from 33 strains of hafnia of different origin, were analysed by sds-polyacrylamide gel electrophoresis. about 21 protein bands could be distinguished in omp of each strain. the major proteins of the outer membrane were of apparent molecular weight 34.000 (34k), 37.000 (37k), 39.000 (39k) and 41.000 (41k). on the basis of the electrophoregrams obtained, four groups of strains possessing different composition of major omp were distinguished: (1) 34k and 3 ...19883254120
hafnia alvei lipopolysaccharides: isolation, sugar composition and sds-page analysis.lipopolysaccharides (lps) of 33 strains of hafnia alvei were isolated and purified. lps content of the dry bacterial mass ranged from 1.2 to 4.5%. all examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some h. alvei strains. sialic acid was present in lps of one strain. d-3-hydroxybutyryl groups also were identified in lipopolysaccharides of 5 ...19883273465
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
hemoglobin hafnia: alpha 2 (beta 116 (g18) his----gln)2; a new hemoglobin variant mistaken for glycated hemoglobin.isoelectric focusing of hemolysate from patients with diabetes mellitus is routinely performed to measure their level of hba1c (glycated hemoglobin). for a 6 year old boy with diabetes mellitus this analysis showed an hba1c fraction of approx. 50%, which is very unlikely to occur. the possibility of a hemoglobin variant was considered, and by hplc-separation the presence of two different beta-chains was shown. one tryptic fragment was found to deviate from the normal, and amino-acid analysis and ...19883395624
a conductance medium to distinguish between salmonella and citrobacter spp.published methods for detecting salmonella by conductance methods yield some citrobacter spp. as false positives. a medium is described which, when used in conjunction with selenite-cystine/trimethylamine oxide/mannitol (sc/t/m) resolves this difficulty. it is based on the ability of salmonella to decarboxylate lysine in the presence of selenite (4 g/l), after pre-enrichment in buffered peptone water supplemented with glucose and lysine for 7-17 h. salmonella produced a conductance change of 250 ...19883152806
crystallization and purification of the enzyme anthranilate phosphoribosyl transferase.anthranilate phosphoribosyl transferase from the bacterium hafnia alvei has been crystallized. this enzyme is one of a small number that constitute the biosynthetic pathway for tryptophan. large cubic crystals were grown at 4 degrees c by dialyzing away the glycerol from a protein solution that included ammonium sulfate, polyethylene glycol and glycerol. the crystals were much more temperature stable and resistant to x-ray deterioration than a previous, similar crystal form that had included gly ...19883199444
[gram-negative flora of horticultural produce destined for consumption mainly in the raw state].a survey has been carried out to evaluate the recovery of enterobacteriaceae in freshly consumed horticultural products. 64 samples of these vegetables random chosen in different stores in the general vegetable market of ferrara have been examined among the families of compositae (lettuce, prickly lettuce, cabbage lettuce, common chicory, artichoke), umbrelliferae (curly parsley, carrot, celery, fennel), cruciferae (garden cabbage, red radish), liliaceae (onion), and solanaceae (tomato). 654 bac ...19892483908
o-specific polysaccharides of hafnia alvei lipopolysaccharides isolated from two serologically related strains: atcc 13337 and 1187. a serological and structural study using chemical methods, gas chromatography/mass spectrometry and nmr spectroscopy at 500 mhz.the o-specific polysaccharides of hafnia alvei atcc 13337 standard strain and 1187 strain have been isolated and characterized. by means of 1h-nmr spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where acyl = d-3-hydroxybutyryl, and 3-o-acetylation was to about 66%. the structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were deter ...19892606108
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
in-vitro interactions of fce 22101 with aminoglycosides against gram-negative rods.agar dilution mics of fce 22101 were measured for 894 consecutively-isolated gram-negative rods from clinical specimens (413 escherichia coli, 104 klebsiella spp., 54 enterobacter spp., 19 citrobacter spp., 131 proteus spp., 43 acinetobacter spp., 9 serratia spp., 3 providencia spp., 4 yersinia spp., 3 hafnia spp. and 111 pseudomonas spp.). excluding pseudomonas spp., 98% of these isolates were susceptible to 8 mg/l fce 22101. resistance (mic greater than 8 mg/l) varied from 11% in serratia spp. ...19892732132
volatile compounds associated with microbial growth on normal and high ph beef stored at chill temperatures.volatile compounds produced by pseudomonas fragi and mixed, natural floras on beef of normal ph (5.5-5.8; glucose greater than 1500 micrograms/g) and high ph (6.3-6.8; glucose less than 10 micrograms/g) included a range of alkyl esters and a number of sulphur-containing compounds including dimethylsulphide but not hydrogen sulphide. production of the last was a property common to the other gram-negative organisms tested viz. hafnia alvei, enterobacter agglomerans, serratia liquefaciens, alteromo ...19892753835
hemoglobinopathies in danish families.based on cases referred for investigation, as well as a questionnaire sent to all medical and pediatric departments in denmark, 48 cases of hemoglobinopathy in 15 families of danish ancestry are reviewed. 18 danes in six families have been identified as having beta-thalassemia, and remarkably one - a homozygote - has beta-thalassemia intermedia requiring treatment with iron-chelation therapy. a further 36 danes in 9 families have a hemoglobin variant: five unstable hemoglobins (volga, niteroi, a ...19892772554
antibacterial activity of tigemonam dicholate (sq 30836) and interaction with beta-lactamases of gram-negative bacteria.sq 30836 is an orally absorbed salt of tigemonam, a new monobactam similar to aztreonam in structure and microbiologic properties. when assayed against 400 clinical isolates, tigemonam's activity was similar to that of aztreonam and carumonam. it was highly effective against enterobacteriaceae but showed poor activity against gram-positive organisms. it inhibited 90% of escherichia coli, klebsiella, shigella, yersinia, proteus, providencia, and morganella strains at 0.5 micrograms/ml or less, an ...19892809698
[serious risk of anesthetic systems built over a hafnia-d-system or a 3-way scuttle. erroneous assembling of anesthetic systems might cause fatal pulmonary damage]. 19892911918
microbiological characteristics of precooked, vacuum-packaged uncured beef and pork.microbiological examination of 29 packages of precooked, vacuum-packaged beef and pork products purchased at local and regional supermarkets along with 110 packages of precooked, vacuum-packaged, sliced roast beef obtained from a major meat processor did not disclose the presence of escherichia coli, coagulase-positive staphylococcus aureus, clostridium perfringens or salmonella. the effects of storage temperature and length of storage on the microflora of 110 packages of sliced roast beef were ...198922056107
optical properties of cubic hafnia stabilized with yttria.the composition, transparency, refractive index, and infrared reflectance of yttria-stabilized cubic hafnia (c-hfo(2)) single crystals were measured. the material is transparent from the ultraviolet to the mid-infrared and for 9.6-mol % y(2)o(3), the index is slightly smaller than for comparable cubic zirconia c-zro(2) or for diamond, but the dispersion (n(f) - n(c) = 0.02811) is larger than that of diamond. the index vs wavelength from 0.36 microm in the ultraviolet to 5.0 microm in the infrare ...199020556154
structural studies on hafnia alvei 114-60 o-antigen.the structure of hafnia alvei 114-60 o-antigen has been established using sugar and methylation analyses as well as 1h-nmr spectroscopy. the results obtained proved that the repeating unit of hafnia alvei 114-60 o-antigen is identical to that of hafnia alvei atcc 13337 standard strain and has the following structure: [formula: see text]19901718234
[the identification of lactose-negative enterobacteria].study of the morphologic and enzymatic characteristics of 10 strains of lactose-negative enterobacteria, sent to the l. a. tarasevich state institute for control and standardization as b. paracoli in order to determine their taxonomic status in accordance with the 1974 and 1984 bergey classifications has referred 9 of these strains to hafnia alvei.19901705611
identification of the bacterium associated with haemorrhagic septicaemia in rainbow trout as hafnia alvei.an unidentified bacterium responsible for epizootic haemorrhagic septicaemic disease in rainbow trout was recently described. this organism exhibited some antigenic similarity to brucella abortus and to yersinia ruckeri and was presumed to belong to the family enterobacteriaceae. further biochemical characterization and dna relatedness studies showed that pathogenic fish strains are hafnia alvei.19902120748
immunogenic and protective properties of outer membrane proteins of hafnia alvei.outer membrane proteins (omp) extracted from antigenically distinct or related strains of hafnia alvei containing defined composition of major proteins proved to be immunogenic. intraperitoneal immunization of mice with a single dose of such preparations protected the animals against homologous and heterologous hafnia strains. the omp preparations were also found to induce protection with varying intensity against escherichia, proteus, shigella and salmonella.19902130804
the normal microbial flora of the outer ear canal in healthy norwegian individuals.the microbial flora of the outer ear canal was determined for 77 healthy individuals (m = 44, f = 33). no growth of any microbe was found in 5% of males and 15% of females. coagulase-negative staphylococci, dominated by staphylococcus epidermidis, were the commonest microbe group found (83% of persons sampled). staphylococcus aureus was found in 7% males and no females. diptheroids were cultured from 32% of the samples. the only gram-negative rod found was hafnia alvei in 4% of individuals. no v ...19902216049
[typical nosocomial infection with an unusual cause: hafnia alvei. report of 2 cases and literature review].the route of infection and the course of two typical nosocomial infections are described in two patients infected with a rare gram-negative bacterium. both patients underwent cardiovascular surgery. they were placed close to each other in the intensive care unit for several days and suffered from pneumonia and from wound infection respectively. in both patients bacterial culture grew hafnia alvei. successful antibiotic treatment was achieved with netilmicin and imipenem. urinary tract, respirato ...19902218242
evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria.a comprehensive phylogenetic tree for virtually the entire assemblage of enteric bacteria is presented. character states of aromatic amino acid biosynthesis are used as criteria, and the results are compared with partial trees based upon sequencing of 16s rrna, 5s rrna, and tryptophan leader peptide. three major clusters are apparent. enterocluster 1 possesses a gene fusion (trpg-trpd) encoding anthranilate synthase: anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase of tryptop ...19902298692
endogenous endophthalmitis due to salmonella arizonae and hafnia alvei.enteric pathogens rarely involve organs other than those of the gastrointestinal system. we have reported the case of a woman with rheumatoid arthritis who had endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. the infection probably resulted from the use of snake powder as a food seasoner. after appropriate intravenous, intraocular, subconjunctival, topical, and oral antibiotic therapy based on laboratory susceptibility studies, the patient's condition clinically improved, ...19902346566
hafnia alvei infection. 19902402660
the structure of the biological repeating unit of the o-antigen of hafnia alvei o39.on mild acid-catalysed degradation of the lipopolysaccharide from hafnia alvei o39 followed by gel filtration of sephadex g-50, the o-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 o-antigen repeating-units. on the basis of sugar and methylation analyses, 13c-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13c-n.m.r. analysis of the smith-degraded polysaccharide, it was conclude ...19901703456
hafnia alvei, a probable cause of diarrhea in humans.hafnia alvei, a member of the family enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. the isolated strain of h. alvei failed to produce heat-labile or heat-stable enterotoxins or shiga-like toxin i or ii and did not invade hela cells, nor did it cause keratoconjunctivitis (determined by the sereny test) in a guinea pig's eye. the strain, however, induced diarrhea in 8 of 12 adult rabbits ...19912004829
etiologic role of hafnia alvei in human diarrheal illness. 19911937837
[hafnia alvei septicemia with shock and dic in an adult with postoperative lung cancer].in japan, we experienced the first case of hafnia alvei septicemia with shock and disseminated intravascular coagulation (dic) in an adult with postoperative lung cancer. a 63 year-old male, who had been followed up in our department since 1987, was admitted to our hospital with the complaints of fever, hemoptysis and dyspnea on june 25, 1989. after admission, he was treated with sulbactam/cefoperazone 4 g/day intravenously for suspicion of respiratory-tract infection. after antibiotic administr ...19911761901
structure of the o-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1h nmr spectroscopy.mild acid hydrolysis of hafnia alvei strain 2 lipopolysaccharide released no o-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with n-acetylneuraminic acid as the reducing terminus. in addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the o-specific polysaccharide chain and the core region, were obtained in minute amounts. on the basis of the sugar and methylation analyses, periodate oxidation, and 1h nm ...19912036370
[the etiologic structure of acute intestinal infections caused by opportunistic microorganisms (irkutsk, 1984-1988)].the authors analyze the results of bacteriologic studies carried out in patients with intestinal infections at the laboratory of the sanitary epidemiologic center of the town of irkutsk over 1984-1988. the records of 24,490 case histories show infections induced by opportunistic microflora in 37 percent of cases. the most frequent etiologic agents were staphylococcus aureus and enterobacteriaceae, genera: proteus, enterobacter, citrobacter, klebsiella, hafnia. analysis of the incidence of acute ...19911715437
o-specific polysaccharide of hafnia alvei lipopolysaccharide isolated from strain 1211. structural study using chemical methods, gas-liquid chromatography/mass spectrometry and nmr spectroscopy.the hafnia alvei strain 1211 o-specific polysaccharide is composed of 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactose, n-acetyl-d-galactosamine, n-acetyl-d-glucosamine and d-glucose (1:1:2:2). on the basis of sugar and methylation analyses, smith degradation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, the polysaccharide was shown to be an o-acetylated polymer of the repeating hexasaccharide unit, ----2d(4-oac)fucp3nacyl beta 1----6dglcpnac alpha 1---- (dglcp beta 1----3)4 ...19911716201
evolution of the ferric enterobactin receptor in gram-negative bacteria.using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ...19911717434
identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test.we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ...19911774276
serological characterization of lipopolysaccharides of hafnia alvei 1, 17, 31, 32, 38 and 39.lipopolysaccharides of hafnia alvei strains 1, 17, 31, 32, 38 and 39 indicate strong cross-reactivity in passive hemagglutination. all lipopolysaccharides were examined by rocket immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting.19911804049
what is the diagnostic value of beta-d-glucuronidase (bdg) activity of bacteria using fluorocult ecd agar for their cultivation?a total of 1510 strains from 15 genera of gram-negative and gram-positive bacteria were studied. more than 94% of 327 escherichia coli strains showed beta-d-glucuronidase (bdg) activity. seventeen serotypes from 170 e. coli o serogroup representatives were negative. relationship between the existence of bdg positive and negative e. coli strains in the same serogroup or serotype has not been observed. the rate of bdg positivity was 42% among salmonella arizonae strains and 42.2% among shigella st ...19911805501
plasma desorption mass spectrometry as a tool in characterization of abnormal proteins. application to variant human hemoglobins.we here report the application of plasma desorption mass spectrometry in combination with reversed-phase high-performance liquid chromatography and automatic edman sequencing for the characterization of hemoglobin variants. by use of plasma desorption mass spectrometry to obtain molecular weight information of purified globin peptides it is possible to minimize the number of candidate positions for substitutions allowing an optimal use of automatic edman degradation. each variant can be characte ...19911812782
[clinical significance of the isolation of hafnia alvei from the feces of children with acute gastroenteritis]. 19911822161
expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco.a possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. the ldcgene from hafnia alvei was therefore (a) placed under the control of the 1' promoter of the bidirectional tr promoter from agrobacterium tumefaciens ...19911883998
esp3i--a novel type iis restriction endonuclease from hafnia alvei that recognizes the sequence 5'-cgtctc(n)1/5-3'. 19911923776
l-prolineaminopeptidase activity as a tool for identification and differentiation of serratia marcescens, serratia liquefaciens and hafnia alvei strains.s. marcescens (316 strains), s. liquefaciens (10 strains) and hafnia alvei (20 strains), in contrast to 18 other enterobacteriaceae species, hydrolyzed l-proline-4-nitroanilide within 30 min at 37 degrees c. in this way, rapid identification of these species is possible. the substrate is applied in solution or in a paper disc. the substrate optimum of hafnia alvei strains proved to be 16 times lower than that of the serratia spp. application of a tenth of the substrate concentration necessary fo ...19911930558
induction of the alka gene of escherichia coli in gram-negative bacteria.a broad-host-range plasmid containing a fusion of the alka and lacz genes of escherichia coli was introduced into various aerobic and facultative gram-negative bacteria--33 species belonging to 19 genera--to study the induction of expression of the alka gene by alkylating agents. the bacteria included species of the families enterobacteriaceae, pseudomonadaceae, rhizobiaceae, vibrionaceae, neisseriaceae, rhodospirillaceae, and azotobacteraceae. results obtained show that all bacteria tested, exc ...19911938974
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...19911657086
the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin).the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ...19911664418
studies on immunity in mice immunized with outer membrane proteins of hafnia.nonspecific protection induced in mice after administration outer membrane proteins of hafnia alvei against infection with homologous and heterologous bacteria was transferred into other mice with lymphocytes isolated from spleens of mice immunized with outer membrane proteins. it was also found that mice sensitized with outer membrane proteins derived from h. alvei or with living bacteria induced in animals delayed hypersensitivity (dth) in homologous and heterologous systems. the observed type ...19921299169
some biological properties of outer membrane proteins of hafnia.outer membrane proteins (omp) isolated from four antigenically different strains of hafnia alvei were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 doses for the studied preparations determined on inbred mice were 18, 20, 28 and 34 mg/kg. these differences in the toxicity of the preparations were reflected in manifestation of shwartzman reaction; more toxic preparations induced strongest necrohemorrhagic ...19921299170
epidemiological role of arthropods detectable in health facilities.a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ...19921350604
[occurrence of p.fimbrii in strains from selected genera of enterobacteriaceae].this study was aimed at recognition of frequency of occurrence of p fimbriae in strains of escherichia coli isolated from samples of feces of children with symptoms of diarrhoea and at search of these adhesions in strains representing other than escherichia genera of enterobacteriaceae strains. one hundred forty laboratory strains were investigated. they belonged to genus citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, salmonella, shigella, and yersinia. also wer ...19921363960
sheen formation and growth response of groundwater bacteria to reduced oxygen concentrations during incubation of m-endo medium.in vitro pure-culture studies were conducted to assess growth and sheen formation of groundwater bacteria on m-endo medium incubated under reduced oxygen concentrations (0, 4, 8, 12, and 16%). coliform and noncoliform bacteria were isolated from 17 untreated, rural groundwater supplies on m-endo medium. all 16 coliform isolates tested were capable of sheen formation at oxygen concentrations of 4% or greater, yet some of these same isolates (enterobacter aerogenes, enterobacter cloacae, and hafni ...19921393829
the structure of a glycerol teichoic acid-like o-specific polysaccharide of hafnia alvei 1205.the o-specific polysaccharide of hafnia alvei 1205 contained d-glucose, d-galactose, 2-acetamido-2-deoxy-d-glucose, 4-acetamido-4,6-dideoxy-d-glucose (qui4nac), glycerol, phosphate, and o-acetyl groups. on the basis of 1d and 2d shift-correlated homonuclear and 13c-1h heteronuclear nmr spectroscopy, methylation analysis, smith degradation, and dephosphorylation with hydrofluoric acid, it was concluded that the o-antigen was a partially o-acetylated teichoic acid-like polysaccharide having the fo ...19921394318
the structure of the o-specific polysaccharide from hafnia alvei strain 38 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide from h. alvei strain 38 has been established by nmr spectroscopy (13c and 1h) and methylation analysis to have the repeating unit-->4)-beta-d-manpnac-(1-->4)-alpha-d-glcpnac(1-->.19921394327
alw26i, eco31i and esp3i--type iis methyltransferases modifying cytosine and adenine in complementary strands of the target dna.the specificity of three dna methyltransferases m.alw26i, m.eco31i and m.esp3i, isolated from acinetobacter iwoffi rfl26, escherichia coli rfl31 and hafnia alvei rfl3+, respectively, was determined. all the enzymes methylate both strands of asymmetric recognition sites yielding m5c in the top-strand and m6a in the bottom-strand, as below: 5'-gtm5ctc 5'-ggtm5ctc 5'-cgtm5ctc 3'-cm6agag 3'-ccm6agag 3'-gcm6agag (m.alw26i) (m.eco31i) (m.esp3i) they are the first members of type iis methyltransferases ...19921408816
sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic escherichia coli and hafnia alvei.seven strains of hafnia alvei isolated from diarrhoeal stools of children resembled enteropathogenic escherichia coli (epec) in that they produced attaching-effacing (ae) lesions in rabbit ileal loops and fluorescent actin staining in infected hep-2 cells. in addition, a dna probe from a chromosomal gene required by epec to produce ae lesions, hybridised to chromosomal dna from all seven h. alvei strains. these findings indicate that there is a sharing of virulence-associated properties at the p ...19921433251
analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry.fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ...19921447059
in vitro activity and beta-lactamase stability of ljc 10,627.the in vitro activity of ljc 10,627, a new carbapenem, was compared with those of imipenem, cefotaxime, ceftazidime, and gentamicin. ljc 10,627 inhibited 90% of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter agglomerans, enterobacter cloacae, hafnia alvei, citrobacter freundii, citrobacter diversus, proteus mirabilis, morganella morganii, proteus rettgeri, serratia marcescens, pseudomonas cepacia, salmonellae, shigellae, aeromonas, and yersiniae ...19921510436
nucleotide sequence of the escherichia coli cad operon: a system for neutralization of low extracellular ph.lysine decarboxylase of escherichia coli has been the subject of enzymological studies, and the gene encoding lysine decarboxylase (cada) and a regulatory gene (cadr) have been mapped. this enzyme is induced at low ph in the presence of lysine and achieves maximal level under anaerobic conditions. the induction of lysine decarboxylase increases the ph of the extracellular medium and provides a distinctive marker in tests of clinical strains. we report the sequence of the cad operon encoding lysi ...19921556085
ru 29 246, the active compound of the cephalosporin-prodrug-ester hr 916. i. antibacterial activity in vitro.the aminothiazolyl-cephalosporin ru 29 246 is the active metabolite of the prodrug-pivaloyl-oxyethyl-ester hr 916. ru 29 246 in vitro activity includes a wide range of clinically relevant bacterial pathogens. against methicillin-sensitive staphylococci ru 29 246 (mic90 of 0.25 approximately 2 micrograms/ml) was clearly more active than cefaclor, cefuroxime, cefpodoxime, cefixime and ceftibuten, but slightly less active than cefdinir. ru 29 246 inhibited hemolytic streptococci of the serogroups a ...19921592683
[colitis and hafnia alvei]. 19921602859
acute bacterial gastroenteritis caused by hafnia alvei. 19921623093
humoral response in mice immunized with outer membrane proteins of hafnia alvei. protective activity of anti-omp-antibodies.intraperitoneal immunization of mice with outer membrane proteins (omp) of hafnia alvei induced in the animals a synthesis of specific antibodies. the antibody levels, determined by elisa test, were found to be relatively low in the sera of mice immunized with a single dose (5 micrograms) of omp and after a second immunization. however, they were higher in mice immunized with three doses of omp. the antibodies were present in circulation for a relatively short time after immunization. serum cont ...19921340186
raman spectrum of zirconia-hafnia mixed crystals. 199210001722
testing of the durability of single-crystal calcium fluoride with and without antireflection coatings for use with high-power krf excimer lasers.the transmission of uncoated and antireflection (ar)-coated caf(2) windows has been measured during long krf laser exposures. samples were tested for up to 75 million pulses in front of an industrial-grade krf excimer laser at high repetition rates (200-300 hz) and moderately high energy fluence (220-550 mj/cm(2)). in this fluence range bare caf(2) performs much better than the best fused silicas currently available. various ar coatings (whose structures were initially unknown to us) were deposi ...199220733809
reactive evaporation of low-defect density hafnia.motivation for this work includes observations at lawrence livermore national laboratory of a correlation between laser damage thresholds and both the absorption and the nodular-defect density of coatings. activated oxygen is used to increase the metal-oxidation kinetics at the coated surface during electron-beam deposition. a series of hafnia layers are made with various conditions: two µ-wave configuations, two sources (hafnium and hafnia), and two reactive oxygen pressures. laser damage thres ...199320856370
multipoint identification of enterobacteriaceae: report of the british society for microbial technology collaborative study.to evaluate the accuracy and reproducibility of multipoint identification schemes in a multicentre trial.19938157751
3-deoxy-octulosonic acid-containing trisaccharide fragment of an unusual core type of some hafnia alvei lipopolysaccharides.the 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of hafnia alvei strains 2 and 1211 using gel filtration. for purification the trisaccharide fraction was rechromatographed on a bio-gel p2 column. on the basis of sugar and methylation analyses and 1h nmr spectroscopy the trisaccharide was identified as 3-deoxy-7-o-(6-o-acetyl-alpha-d-galactopyranosyl)-4-o-(alpha-l-glycero-d - mannoheptopyran ...19937688960
a novel strategy for the isolation of luxi homologues: evidence for the widespread distribution of a luxr:luxi superfamily in enteric bacteria.the pheromone n-(3-oxohexanoyl)-l-homoserine lactone (ohhl) regulates expression of bioluminescence in the marine bacterium vibrio fischeri, the production of carbapenem antibiotic in erwinia carotovora and exoenzymes in both e. carotovora and pseudomonas aeruginosa. a characteristic feature of this regulatory mechanism in v. fischeri is that it is cell density-dependent, reflecting the need to accumulate sufficient pheromone to trigger the induction of gene expression. using a lux plasmid-based ...19937968529
[the detection of antagonistic strains in representatives of the genera bacillus and hafnia]. 19938079538
[studies of humoral immunity induced by proteins of the cell wall of hafnia alvei].it was found that cell wall proteins (cwp) of hafnia alvei applied intraperitoneally to mice are inducing, beside previously detected cellular immunity, also humoral immunity. applying elisa immunoenzymatic test in which a conjugate enabling demonstration of igg antibodies was applied, it was found that cwp of hafnia alvei induce appearance of specific antibodies in a high titer, but their presence in mouse serum is short and independent from applied doses of cwp. humoral immunity induced by cwp ...19938189802
study of enterobacteriaceae throughout the manufacturing and ripening of hard goats' cheese.the evolution of the counts and the species of enterobacteriaceae as well as some physico-chemical parameters (ph, aw and nacl and moisture contents) during manufacturing and ripening of a hard spanish goats' cheese of the armada-sobado variety were studied. enterobacteriaceae (mean log counts 4.45 g-1 in milk) increased 0.71-2.18 log units in curd and afterwards decreased until they disappeared after 2-4 weeks of ripening. this premature disappearance seems to be due to the decrease in aw value ...19938244902
ceftazidime resistance in hafnia alvei.two morphotypes of hafnia alvei differed in their susceptibilities to beta-lactam antibiotics. both produced an inducible bush group 1 beta-lactamase. hyperinducibility of this enzyme was associated with reduced susceptibility in one morphotype.19938328790
a multidrug resistance regulatory chromosomal locus is widespread among enteric bacteria.constitutive expression of the mar operon (marrab) in escherichia coli produces a multiple antibiotic resistance phenotype mediated by the expression of multiple genetic loci in response to regulatory proteins in the operon. a mar-specific dna probe was used to search for the operon in bacterial strains representing 53 species and 27 genera. among these, 6 other enterobacteriaceae, salmonella, shigella, klebsiella, citrobacter, hafnia, and enterobacter species, contained dna sequences that hybri ...19938335992
nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of escherichia coli.arginine decarboxylase (encoded by adi) is induced under conditions of acidic ph, anaerobiosis, and rich medium. the dna sequence of a 3-kb fragment of the escherichia coli chromosome encoding biodegradative arginine decarboxylase was determined. this sequence encodes a protein of 755 amino acids with a molecular size of 84,420 daltons. the molecular weight and predicted adi amino acid composition agree with those found in earlier work. the amino acid sequence of arginine decarboxylase showed ho ...19938383109
analysis of 7-substituted sialic acid in some enterobacterial lipopolysaccharides.sialic acid-containing lipopolysaccharides (lps) were isolated from six bacterial strains of the family enterobacteriaceae. sialic acid was released from permethylated lps by methanolysis, and partially o-methylated n-acetyl-n-methyl-neuraminic acid methyl ester methyl glycosides were analyzed by gas-liquid chromatography-electron ionization mass spectrometry. it was proved that all lps contain n-acetylneuraminic acid (neuac). the occurrence of 7-substituted neuac in escherichia coli serotypes o ...19938444811
acute gastroenteritis caused by hafnia alvei in children. 19938452958
structure of the o-specific polysaccharide containing pentitol phosphate, isolated from hafnia alvei strain pcm 1191 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide produced by hafnia alvei strain pcm 1191 was shown by composition and methylation analyses, periodate oxidation and one- and two-dimensional 1h- and 31p-nmr spectroscopy to be a polymer of a branched hexasaccharide repeating units having the structure: [formula: see text] where laraol = l-arabitol the polysaccharide of 1191 strain has teichoic-acid-like character. its peculiar feature is the presence of arabitol phosphate, a component obser ...19938504816
phenotypic diversity of anaerobic glycerol dissimilation shown by seven enterobacterial species.the anaerobic glycerol pathway was studied in seven enterobacterial species selected as representative of different behaviours in terms of anaerobic glycerol dissimilation. the presence of oxidative and reductive pathways of the dha regulon in klebsiella pneumoniae enabled the cells to grow fermentatively on glycerol. the first two enzymes of the dha regulon (glycerol dehydrogenase type i and dihydroxyacetone kinase) represent the oxidative branch, while the latter two (glycerol dehydratase and ...19948090993
serological and structural features of hafnia alvei lipopolysaccharides containing d-3-hydroxybutyric acid.the serological heterogeneity of hafnia alvei lipopolysaccharides from strains atcc 13337, 1187, 1221, 114/60, 1211 and 1216, that contain d-3-hydroxybutyric acid, was analyzed by rocket immunoelectrophoresis, immunoblotting and passive hemagglutination. the significance of d-3-hydroxybutyric acid component for their cross-reactivity has been discussed. the results obtained allowed us to place four h. alvei strains (atcc 13337, 1187, 1221 and 114/60) in one serotype (a) and to consider two other ...19948156055
[hafnia alvei isolated in acute gastroenteritis]. 19947996884
reactive arthritis associated with hafnia alvei enteritis. 19948003071
hafnia alvei in stool specimens from patients with diarrhea and healthy controls.we found an epidemiological association of hafnia alvei with diarrhea, because the organism was isolated from 12 of 77 (16%) adult finnish tourists to morocco who developed diarrhea and from 0 of 321 tourists without diarrhea (p < 0.001). from another group of 112 adult finnish diarrheal patients, only 2 (2%) yielded h. alvei. in contrast to some bangladeshi strains of h. alvei, the finnish strains were negative for the attachment-effacement lesion by an in vitro fluorescent acting staining test ...19947814573
[the heterogeneity of the antilysozyme activity in populations of opportunistic enterobacteria].1,484 clones of 104 strains representing 11 genera of the family enterobacteriaceae and having different levels of lysozyme activity were tested for the presence of this activity. in the populations of 53.9% of strains under study the heterogeneity of the levels of antilysozyme activity was noted. in 13.4% of strains under study clones having no antilysozyme activity were detected. among escherichia, hafnia and enterobacter strains without antilysozyme activity individual clones having such acti ...19947856344
comparison of the antibody responses to the 77 klebsiella capsular types in ankylosing spondylitis and various rheumatic diseases.the production of antibodies to klebsiella capsular polysaccharides was measured in sera from either hla-b27-positive (hla-b27+) or hla-b27-negative (hla-b27-) patients with classical ankylosing spondylitis (n = 54). these sera were compared with sera from patients with various rheumatic diseases (n = 82) and hla-b27+ or hla-b27- healthy individuals (n = 85). all sera were analyzed by means of an enzyme-linked immunosorbent assay specific to each of the 77 klebsiella serotypes. the sera from hla ...19947927763
unique fimbriae-like structures encoded by sefd of the sef14 fimbrial gene cluster of salmonella enteritidis.the sef14 gene cluster of salmonella enteritidis was recently shown to contain three genes, sefabc, encoding a unique fimbrin, and proteins homologous to fimbrial chaperones and outer membrane proteins (ushers), respectively. a fourth open reading frame, designated sefd, was found immediately downstream of sefabc and overlapping sefc. the translated protein sequence of sefd was unique, but the composition was similar to that of other bacterial fimbriae. sefd was produced in abundance by wild-typ ...19947934897
the structure of a core oligosaccharide component from hafnia alvei strain 32 and 1192 lipopolysaccharides. 19947511987
the structure of the o-specific polysaccharide of hafnia alvei strain 1216.the o-specific polysaccharide of hafnia alvei strain 1216 is composed of d-galactose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucose, and o-acetyl groups in the ratios 1:1:2:1:1. on the basis of sugar and methylation analyses of the intact and chemically degraded (o-deacetylated, carboxyl-reduced, smith-degraded) polysaccharide and 1h and 13c nmr spectroscopy, including 2d shift-correlated (cosy, relayed cosy, 13c, 1h-cosy) and 1d noe spectros ...19947518747
hafnia alvei. respiratory tract isolates in a community hospital over a three-year period and a literature review.in a retrospective review, a group of seven patients were found to have a sputum culture positive for hafnia alvei. hafnia alvei is a gram-negative enteric and oropharyngeal bacillus and usually is nonpathogenic. all our patients had a chronic underlying illness and one of the patients was endotracheally intubated at the time of the isolation of this organism. six of seven patients had other organisms isolated along with h alvei, and only one patient had a pure growth of h alvei confirmed by a c ...19948162732
characterization of the c-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic escherichia coli, citrobacter freundii, and hafnia alvei.surface proteins called intimins (int), which are homologous to the invasin protein (inv) of yersinia spp., play a role in inducing brush border damage, termed attachment and effacement, which follows infection by enteropathogenic and enterohemorrhagic escherichia coli, citrobacter freundii biotype 4280, and hafnia alvei. maltose-binding protein (mbp) fusions containing the c-terminal 280 amino acids of int-like proteins of strains of enteropathogenic e. coli, enterohemorrhagic e. coli, h. alvei ...19948168946
biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts.we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ...199416349260
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...199516535048
3-deoxy-octulosonic-acid-containing hexasaccharide fragment of unusual core type isolated from hafnia alvei 2 lipopolysaccharide.the hexasaccharide containing 3-deoxy-octulosonic acid (kdo) was isolated from the carbohydrate material obtained after the mild acid hydrolysis of lipopolysaccharide of hafnia alvei strain 2. the hexasaccharide was purified by gel filtration on bio-gel p-4 and p-2 columns. on the basis of sugar and methylation analyses, one- and two-dimensional nmr spectroscopic methods, the hexasaccharide was identified as [formula: see text] the tetrasaccharide linked to position 7 of kdo is also part of the ...19957867651
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