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imipenem coadministered with cilastatin compared with moxalactam: integration of serum pharmacokinetics and microbiologic activity following single-dose administration to normal volunteers.we administered 1 g of imipenem along with equal amounts of cilastatin (a dehydropeptidase i inhibitor) or 2 g of moxalactam intravenously over a period of 30 min to six volunteers in a crossover manner 1 week apart. the antibiotic concentrations and pharmacokinetics for each drug were determined and integrated with the microbiologic activity by measuring the duration of time that the free drug concentrations remained above the mics for 90% of 581 clinical isolates and by measuring serum bacteri ...19863459390
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria.the iron uptake test of szabó and vandra has been modified and used for the differentiation of gram-negative bacteria. nutrient agar containing 20 g per litre of ferric ammonium citrate was distributed into narrow tubes and solidified so as to form butts and slants. considering the localization of the rusty-brown coloration produced after seeding and incubation, 2367 strains were classified into four groups. (1) unchanged medium: escherichia coli, shigella spp., yersinia spp., hafnia alvei and m ...19863529797
pneumatosis intestinalis in children after allogeneic bone marrow transplantation.four children, ages 3 to 8 years, developed pneumatosis intestinalis (pi) after allogeneic bone marrow transplantation (bmt) for acute leukemia or severe aplastic anemia. pi was detected at a median of 48 days (range, 10-63 days) after bmt and was associated with abdominal symptoms and clinical signs. all patients had severe systemic and/or high-grade cutaneous acute graft-versus-host disease (agvhd) at some time after bmt and were receiving corticosteroids at the time of development of pi; howe ...19873547276
enterobacteriaceae, coliforms, faecal coliforms and salmonellas in raw ewes' milk.thirty-nine samples of freshly drawn ewes' milk collected at 13 farms, and 120 samples of raw ewes' milk collected on arrival at a dairy were examined. farm samples had geometric mean counts of 4.4 x 10(2) enterobacteriaceae/ml, 3.9 x 10(2) coliforms/ml and 2.0 x 10(2) faecal coliforms/ml, whereas the respective mean counts were 6.2 x 10(3)/ml, 5.4 x 10(3)/ml and 1.3 x 10(3)/ml for dairy samples. salmonellas were not detected by enrichment procedures in any of the 159 samples examined. escherich ...19873597207
the application of 1h/13c inversely correlated nmr spectroscopy to the determination of acylation and glycosylation sites in the o-specific polysaccharide from hafnia alvei 1187.an inversely correlated 1h/13c nmr spectrum defined the amino sugars acylated by acetyl or 3-hydroxybutyryl groups and revealed partial sequences and glycosylation sites in a tetrasaccharide repeating unit of the title polysaccharide, (----2dglc alpha 1----3dglcnacyl alpha 1----4dgalnac alpha 1----3dgalnac beta 1----)n, where acyl = 3-hydroxybutyryl.19873619933
o-nitrophenyl-beta-d-galactopyranoside-urease-indole broth, a new composite tube medium for salmonella screening.a new composite broth medium combining o-nitrophenyl-beta-d-galactopyranoside (onpg) and urease and indole tests in a single tube is described. high-level agreement with individual conventional tests was recorded in comparative studies with 2,412 cultures of members of the family enterobacteriaceae, i.e., 100% agreement with the exception of hafnia spp. (96.3% agreement) for the onpg test and citrobacter, enterobacter, and hafnia spp. (75, 86.4, and 98.2% agreement, respectively) for the urease ...19863639886
inhibition of yersinia enterocolitica serotype o3 by natural microflora of pork.yersinia enterocolitica serotype o3 did not grow but did survive in inoculated raw ground pork kept at 6 and 25 degrees c. the antagonistic effect of microbial flora, especially hafnia alvei and environmental yersinia organisms, on the growth of y. enterocolitica serotype o3 in raw ground pork was evident. these results were supported by evidence of the inhibition of growth of y. enterocolitica serotype o3 by enterobacteriaceae, especially h. alvei and environmental yersinia organisms, in mixed ...19863729395
bacterial sources of putrescine and cadaverine in chill stored vacuum-packaged beef.of the meat strains of streptobacteria, leuconostocs, enterobacteriaceae and brochothrix thermosphacta tested, only hafnia alvei and serratia liquefaciens showed diamine-producing potential during growth in pure culture on beef stored in vacuum packs at 1 degree c. both organisms produced cadaverine at concentrations similar to those reported previously in naturally contaminated beef stored under the same conditions. putrescine concentrations produced by the two organisms, however, were an order ...19863771410
rapid catalase supplemental test for identification of members of the family enterobacteriaceae.a simple, rapid, semiquantitative slide catalase test useful for differentiating members of the family enterobacteriaceae is described. judging by the time required for appearance of oxygen bubbles in 3% hydrogen peroxide, the immediate catalase reactors were yersinia, serratia, proteus, morganella, providencia, cedecea, and hafnia spp. the delayed catalase reactors were escherichia, shigella, klebsiella, enterobacter, salmonella, citrobacter, edwardsiella, kluyvera, and tatumella spp. this info ...19873818934
koserella trabulsii, a new genus and species of enterobacteriaceae formerly known as enteric group 45.the name koserella trabulsii is proposed for a group of enterobacteriaceae formerly called enteric group 45. this group consists of 12 strains that were originally identified as atypical hafnia alvei. k. trabulsii strains were negative for indole production, voges-proskauer, h2s production, urea hydrolysis, phenylalanine deaminase, and acid production from glycerol, lactose, sucrose, and d-sorbitol; they were positive for methyl red, citrate (simmons), lysine and ornithine decarboxylases, argini ...19853968202
[identification of hafnia bacteria]. 19724124730
relation of beta-lactamase activity and cellular location to resistance of enterobacter to penicillins and cephalosporins.the enterobacter species e. aerogenes, e. cloacae, and e. hafnia were examined for resistance to penicillin and cephalosporin derivatives. all were resistant to benzyl penicillin, ampicillin, 6 [d(-)alpha-amino-p-hydroxyphenylacetamido] penicillanic acid, cephaloridine, cephalothin, and cephalexin. a significant number were sensitive to carbenicillin and 6 [d(-)alpha-carboxy-3-thienylacetamido] penicillanic acid. no differences among the three species were noted. the beta-lactamase activity was ...19724218941
[the provisional genus hafnia and its systemic classification within the tribe enterobacteriaceae]. 19664224560
[a provisional diagnostic scheme for differentiating hafnia bacteria according to their o-antigens]. 19744454845
[enzyme characteristics of bacteria of the genera citrobacter and hafnia and their identification during laboratory diagnosis of intestinal infections]. 19744454846
on the taxonomy of the genus hafnia. 19684874471
further use of deoxyribonuclease in a screening test for serratia.the deoxyribonuclease (dnase) test is useful in classifying serratia bacilli and in differentiating it from the hafnia group, e. cloacae, and e. liquefaciens. it is recommended as a differential test in enteric bacteriology because of its specificity, the convenience of its use, and the rapidity with which it can be performed.19684880362
taxonomic investigations on expressed and cryptic phospho-beta-glucosidases in enterobacteriaceae.in the enterobacteriaceae, beta-glucosides are catabolized by a complex system formed of three permeases, with partly overlapping substrate specificities, and two hydrolytic enzymes, phospho-beta-glucosidase a and b, which hydrolyze only phosphorylated beta-glucosides. some enterobacteriaceae such as klebsiella-aerobacter (enterobacter) possess the complete system; others possess only parts of it or may have a cryptic phospho-beta-glucosidase activity without permease activity. a screening test ...19694897109
[characteristics of acute gastro-intestinal diseases caused by bacteria of the genus hafnia]. 19725069112
simplified differentiation from the aerobacter and paracolobactrum (hafnia) groups. 19675342710
[the biochemical properties of bacterial of the genus hafnia]. 19705503341
hafnia septicemia. 19715552027
diagnostic value of a hafnia-specific bacteriophage. 19685674062
[on various strains of hafnia isolated from food]. 19685756724
persistent septicemia due to hafnia alvei. report of a case. 19695770669
[taxonomic position of hafnia in the family enterobacteriaceae]. 19655852423
use of the deoxyribonuclease test as an aid in the differentiation of paracolobactrum (hafnia) from serratia. 19665903113
on the antigenic relationships of certain citrobacter and hafnia cultures. 19665953824
hafnia: an unusual cause of postoperative gram-negative bacteriae. 19676018152
[a new modification of the hafnia circle]. 19676021366
soda-lime consumption in the hafnia respiratory circuit. 19676056543
[interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)].gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ...19806104464
in-vitro activity of the novel oxa-beta-lactam antibiotic moxalactam (ly 127935) against dense populations of selected gram-negative bacilli.the activity of moxalactam, a new beta-lactam antibiotic with a novel molecular structure, was examined against dense populations of a variety of gram-negative bacilli. the lytic activity of moxalactam against ampicillin-sensitive strains was similar to ampicillin, but the activity was maintained against ampicillin-resistant strains, including pseudomonas aeruginosa, irrespective of whether the ampicillin resistance was 'intrinsic' (non-enzymic) or due to beta-lactamase. of the 18 strains tested ...19816265424
[genetic transfer of pap38, pap39, and pap41 plasmids into e. coli, erwinia, and hafnia strains].genetic transfer factors pap38, pap39 and pap41 can be transferred to e. coli, both typed and untypecd, as well as to erwinia and hafnia strains, with different frequency (10(-2) to 10(-8)). the transconjugates thus obtained possess donor activity and can transfer the factors they have received to recipient e. coli strains. after transfer these factors are stably maintained in a new host for at least 10 days. under the action of ethidium bromide or elevated temperature the elimination of the tra ...19826295035
[in vitro bacteriostatic activity of cefmenoxime (sce 1365), cefotaxime and moxalactam].the in vitro activity of cefmenoxime (sce 1365), a new cephalosporin derivate was compared with two other "third generation" cephalosporins: cefotaxime and moxalactam. cefmenoxime as cefotaxime and moxalactam were very active against 305 cephalosporinase-producing and cephalosporinase-non-producing enterobacteriaceae. cefmenoxime was the most active against serratia marcescens, citrobacter freundii, morganella morganii, salmonella, shigella and yersinia enterocolitica with a mean mic at least tw ...19836312395
evaluation of the updated ms-2 bacterial identification system in comparison with the api 20e system.the recently updated ms-2 bacterial identification system software (abbott laboratories, diagnostic division, irving, tex.) was compared with the original ms-2 bacterial identification software and the api 20e, using 968 strains of enterobacteriaceae. the updated ms-2 software correctly identified 94.4% of the isolates tested. api 20e and the original ms-2 software correctly identified 91 and 85.3% of the strains, respectively. ms-2 responses were considered to be equivocal (needing additional t ...19836350342
[influence of hemolytic plasmids on the development of the "killing" effect in various species of enterobacteria].experiments on isogenic strains of different enterobacterial species, both carrying hemolytic plasmids and free from them, have demonstrated that their "killing" effect on mice is linked with the yield of hemolysin and does not depend on the origin of hly plasmids or the chemotype of bacteria. plasmid p ie 567 coding the synthesis of alpha-hemolysin has been shown to be capable of expression in enterobacter aerogenes, hafnia alvei and salmonella typhimurium strains, but e. aerogenes 3/43, h. alv ...19836356727
effect of chlorination on antibiotic resistance profiles of sewage-related bacteria.a total of 1,900 lactose-fermenting bacteria were isolated from raw sewage influent and chlorinated sewage effluent from a sewage treatment plant, as well as from chlorinated and neutralized dilute sewage, before and after a 24-h regrowth period in the laboratory. of these isolates, 84% were resistant to one or more antibiotics. chlorination of influent resulted in an increase in the proportion of bacteria resistant to ampicillin and cephalothin, the increase being most marked after regrowth occ ...19846476832
[study of lincomycin concentrations in hepatocystic duct].penetration of lincomycin (lcm) in choledochal and cholecystic bile as well as in the gallbladder tissue and liver tissue was investigated together with bacteria detectable in the bile in order to evaluate basically usefulness of this antibiotic in the treatment of infections of the hepatocystic duct. intravenous drip infusion of lcm 1.5 g (in 500 ml of 5% glucose solution) over 1.5--2 hours resulted in mean drug concentrations of 33.9 and 10.1 micrograms/ml in serum at 2 and 4 hours post start ...19846502929
kinetic mechanism and location of rate-determining steps for aspartase from hafnia alvei.coupled spectrophotometric assays that monitor the formation of fumarate and ammonia in the direction of aspartate deamination and aspartate in the direction of fumarate amination were used to collect initial velocity data for the aspartase reaction. data are consistent with rapid equilibrium ordered addition of mg2+ prior to aspartate but completely random release of mg2+, nh4+, or fumarate. in addition to mg2+, mn2+ can also be used as a divalent metal with vmax 80% and a kaspartate 3.5-fold l ...19846509019
retardation of wool growth in merino sheep caused by bacteria.a condition evidenced by retarded growth of wool with alteration of the yolk into a yellow, sticky, wax-like substance was investigated. the condition was associated with hyperaemia and cellular infiltration into the dermis in the affected areas. three bacterial species, viz. enterobacter aerogenes, e. agglomerans and hafnia alvei, which could grow on the water-extractable component of wool-yolk, were incriminated as the cause of the condition.19836676689
comparison of three methods for identification of enterobacteriaceae.this study compares the ability of three commercial overnight methods, api 20e, minitek and enteric-tek, to accurately and completely identify 368 clinically isolated enterobacteriaceae without supplemental tests. organisms included escherichia coli (54 strains), shigella spp. (7), edwardsiella tarda (1), salmonella enteritidis (10), citrobacter spp. (30), klebsiella spp. (55), enterobacter spp. (68), hafnia alvei (2), serratia spp. (33), proteus spp. (64), morganella morganii (24), providencia ...19826756905
control of carbon dioxide in modified mapleson a and d (hafnia) anaesthetic systems. an experimental model.the effects of varying ventilations (ve) and fresh gas flows (fgf) on end-expiratory co2 (feco2) levels were investigated in an experimental model lung, employing the hafnia modification of the mapleson a and d anaesthetic systems during co2-absorption and co2-wash-out (rebreathing). identical results were found in both systems: feco2 was constant and independent of fgf with co2-absorption and constant ve, whereas rebreathing resulted in increasing feco2 levels as fgf was decreased. as control o ...19816797219
rhodanese activity: a simple and reliable taxonomic tool for gram-negative bacteria.the thiosulphate: cyanide sulphurtransferase (rhodanese) test of vandenbergh, bawdon and berk (1979) has been simplified and 2469 strains from a wide variety of sources representing different biochemical, serological or phage-pattern entities were tested. the percentages of rhondanese-producing strains were: escherichia coli 98%, shigella flexneri serovars 1-5%, x and y 0%, other shigellae 73-100%, yersinia spp. 0%, salmonella subgenera i-iv 0%, citrobacter freundi 16%, klebsiella 37%, enterobac ...19826958873
evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures.a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ...19806999020
[antigenic relations between enteropathogenic escherichia o151:k, citrobacter and hafnia].the strains isolated from acute dysentery patients and convalescents and identified as e. coli o151:k--were studied. diagnostic errors due to inexact genus determination were revealed, and the isolated strains were found to belong to citrobacter and hafnia. the main biochemical differences allowing to differentiate e. coli o151:k--from these two genera were established. the study also showed the necessity of some tests, such as the tests for citrate assimilation in simmons' medium and sodium mal ...19817023147
studies on virulence of shigella flexneri. protective effect of outer membrane proteins.groups of mice were immunized intraperitoneally with proteins isolated from outer membrane of virulent strain of shigella flexneri 3a and its avirulent variant. all the animals were found to survive challenge with ld100 of virulent sh. flexneri 3a. similar protective effect against challenge with sh. flexneri 3a gave also immunization of mice with outer membrane proteins isolated from sh. flexneri of other serotypes (2a, 4a, 6, and x) and from escherichia coli, hafnia alvei and shigella sonnei.19817025793
[review [new antibiotics series i]: cefuroxime (author's transl)].cefuroxime (cxm) is a new injectable cephalosporin stable against beta-lactamases. the results of preclinical and clinical studies so far carried out in japan on cefuroxime are summarized in this paper. 1. cxm is stable to various kinds of beta-lactamases except the one produced by p. vulgaris gn 76 (richmond type ic). reflecting this action, cxm has been shown to have antibacterial activity, not only against organisms which respond to conventional cephalosporins, but also against citrobacter, e ...19827045446
comparison of two methods for same-day identification of enterobacteriaceae.two commercial methods, api 20e (as modified for same-day enterobacterial identification) and micro-id, were evaluated for ability to provide useful same-day information of 368 clinically isolated enterobacteriaceae. organisms included escherichia coli (54), shigella (7), edwardsiella tarda (1), salmonella enteritidis (10), citrobacter (30), klebsiella (55), enterobacter (68), hafnia alvei (2), serratia (33), proteus (64), morganella morganii (24), providencia (18), and yersinia enterocolitica ( ...19827051807
[antibiotic sensitivity of opportunistic bacteria isolated from patients].sensitivity of 147 enterobacteria strains isolated from feces of various patients was determined with the method of serial dilutions on solid nutrient media. 8 antibiotics were tested. by genera (species) the microorganisms were arranged in the following order: e. coli (65 strains), citrobacter (33 strains), e. cloacae (15 strains), serratia liquefaciens (9 strains), hafnia (6 strains), klebsiella (4 strains), pectobacterium (3 strains), non-identified organisms (13 strains). the majority of the ...19827137975
[heterogeneity of the fatty acid composition of bacteria in the genera klebsiella, enterobacter, hafnia and serratia].the analysis of the chromatograms of methyl esters of fatty acids in bacterial strains of the tribe klebsielleae showed the heterogeneity of the fatty acid composition of bacteria belonging to the genera klebsiella, enterobacter, hafnia and serratia. the bacteria of this tribe could be subdivided into 5 provisory groups in accordance with the composition and profile of their fatty acids. group i comprised k. pneumoniae k1, k. ozaenae and k. rhinoscleromatis strains forming a separate group chara ...19827158162
enterobacteria differentiated by gas-liquid chromatography of metabolites.the short chain acids of 47 strains within the family enterobacteriaceae grown on prereduced, anaerobically sterilized peptone yeast-extract glucose broth (pras py g) under standardized conditions were determined by gas-liquid chromatography (glc). the bacteria could be subdivided into six groups on the basis of their metabolites. acetate, lactate, succinate and ethanol were detected from all strains and constituted a set of basic fermentation products, p, mirabilis, in addition, produced propan ...19817198354
a case with severe diarrhoea and strongyloides stercoralis infection.a case with severe diarrhoea and strongyloides stercoralis infection is described. further examination showed that the patient also had abnormal colonization of the duodenum with hafnia alvei and that this disappeared when the strongyloides infection was treated with mebendazole. symptoms such as abdominal pain, diarrhoea, "skin rash" and malabsorption in association with blood eosinophilia should arouse suspicion of strongyloidiasis.19817234512
aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans.garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ...19817240404
activity of a new cephalosporin antibiotic, ro 13-9904 against dense populations of selected enterobacteria.ro 13-9904 is a new cephalosporin antibiotic with a wide spectrum of activity similar to that of cefotaxime and some other newer beta-lactam compounds. in the present study, the response to ro 13-9904 of 13 strains of enterobacteria, selected on the basis of differential patterns of susceptibility to beta-lactam agents, was investigated. the new cephalosporin displayed high intrinsic activity against most of the enterobacteria tested, but a group of klebsiella and hafnia alvei strains was identi ...19817247361
calculation of the fresh gas flow requirements of the hafnia a and d anaesthetic circuits.semi-closed anaesthetic circuits are converted into the corresponding hafnia circuits by replacing the expiratory valve by a side tube connected to an ejector flowmeter. theoretical analysis of the hafnia a and d circuits revealed by the fresh gas flow requirements are dependent on the inspiration/expiration time ratio. using a ratio of 1/1.2 and a sine-wave respiratory waveform, the minimal fresh gas requirements were calculated as 2.1 (hafnia a) and 2.5 (hafnia d) times the respiratory minute ...19807376802
attributes of microbial associations of meat growing as xenic batch cultures in a meat juice at 4 degrees c.strains of gram-positive (carnobacterium piscicola, leuconostoc mesenteroides subsp. mesenteroides, brochothrix thermosphacta) and gram-negative (pseudomonas fragi and hafnia alvei) bacteria isolated from minced lamb packaged under a modified atmosphere were cultivated in a meat (lamb) juice at 4 degrees c. carbohydrates were catabolised in the order glucose > glucose 6-phosphate during the development of the population. under an atmosphere enriched with carbon dioxide the gram-negative portion ...19957488524
characterization of hafnia alvei by biochemical tests, random amplified polymorphic dna pcr, and partial sequencing of 16s rrna gene.hafnia alvei strains which possess the attachment-effacement gene (eaea) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other h. alvei strains. we characterized diarrheal h. alvei strains, which were positive in the pcr test for the eaea gene, using biochemical tests not routinely used for identification of members of the family enterobacteriaceae, and compared them with eaea-negative strains isolated from different clinical and nonclin ...19957494030
the structure of a core oligosaccharide component from hafnia alvei strain 32 and 1192 lipopolysaccharides. 19947511987
the structure of the o-specific polysaccharide of hafnia alvei strain 1216.the o-specific polysaccharide of hafnia alvei strain 1216 is composed of d-galactose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucose, and o-acetyl groups in the ratios 1:1:2:1:1. on the basis of sugar and methylation analyses of the intact and chemically degraded (o-deacetylated, carboxyl-reduced, smith-degraded) polysaccharide and 1h and 13c nmr spectroscopy, including 2d shift-correlated (cosy, relayed cosy, 13c, 1h-cosy) and 1d noe spectros ...19947518747
lipopolysaccharide core region of hafnia alvei: structure elucidation using chemical methods, gas chromatography-mass spectrometry, and nmr spectroscopy.sugar and methylation analysis with the use of gas chromatography-mass spectrometry and 1h nmr spectroscopy proved that the core oligosaccharides isolated from lipopolysaccharides of eight hafnia alvei strains have the identical hexasaccharide skeleton. however, 1h, 31p heterocorrelated spectra showed that the phosphorylation pattern is not the same. the branched heptose for the atcc 13337, 1187, 2, 1191, 1196, 1220, and 481l strains is phosphorylated as in the following formula, where p = -o-p( ...19957535188
structural studies of the o-specific chain and a core hexasaccharide of hafnia alvei strain 1192 lipopolysaccharide.the structure of the o-specific side-chain and a core hexasaccharide of the hafnia alvei strain 1192 lipopolysaccharide has been investigated. methylation analysis, nmr spectroscopy, maldi-tof spectrometry, and various specific chemical degradations were the principal methods used. it is concluded that the polysaccharide is composed of hexasaccharide repeating-units having the following structure which is partially o-acetylated in the 2-position of the --> 4)-alpha-d-glc pa-(1-->(70%) and on dif ...19957539717
buttock abscess due to hafnia alvei. 19957620037
structural and serological characterization of hafnia alvei lipopolysaccharide core region.the structures and serological activities of core oligosaccharide of hafnia alvei strains have been investigated. methylation analysis, nmr spectroscopy and various specific degradation procedures were the principal methods used. it is concluded that, core hexasaccharides are identical in the lipopolysaccharides tested and are built of two glucose, three heptose and one 2-keto-3-deoxyoctulosonic acid residues. the antiserum raised against the atcc13337 oligosaccharide core-tetanus toxoid conjuga ...19957653161
3-deoxy-octulosonic acid-containing trisaccharide fragment of an unusual core type of some hafnia alvei lipopolysaccharides.the 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of hafnia alvei strains 2 and 1211 using gel filtration. for purification the trisaccharide fraction was rechromatographed on a bio-gel p2 column. on the basis of sugar and methylation analyses and 1h nmr spectroscopy the trisaccharide was identified as 3-deoxy-7-o-(6-o-acetyl-alpha-d-galactopyranosyl)-4-o-(alpha-l-glycero-d - mannoheptopyran ...19937688960
lipopolysaccharide core region of hafnia alvei: serological characterization.covalent glycoconjugates containing, as a ligand lipopolysaccharide core, oligosaccharides of hafnia alvei standard strain atcc 13337 and r mutant 1 m were used to produce anti-h. alvei core antibodies. the sera obtained were tested in rocket immunoelectrophoresis, immunoblotting and elisa using h. alvei lipopolysaccharides of various strains. the experiments were carried out to study the antigenic relationships between lipopolysaccharide core regions in the h. alvei genus.19957719279
structural elucidation of the biological repeating unit of o-specific polysaccharide from citrobacter serotype o41.sugar analysis of the o-specific polysaccharide produced by citrobacter serotype o41 revealed the presence of a hexasaccharide repeating unit, which includes the unusual 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactosyl residue (fuc3nacyl). the structure of the repeating unit was determined by extensive use of homo- and heteronuclear two-dimensional nmr spectroscopy, including the application of long-range 1h-13c correlation experiments and noe studies to establish the sequence of sugar u ...19957744044
acid-base chemical mechanism of aspartase from hafnia alvei.an acid-base chemical mechanism is proposed for hafnia alvei aspartase in which a proton is abstracted from c-3 of the monoanionic form of l-aspartate by an enzyme general base with a pk of 6.3-6.6 in the absence and presence of mg2+. the resulting carbanion is presumably stabilized by delocalization of electrons into the beta-carboxyl with the assistance of a protonated enzyme group in the vicinity of the beta-carboxyl. ammonia is then expelled with the assistance of a general acid group that t ...19957793969
hafnia alvei in stool specimens from patients with diarrhea and healthy controls.we found an epidemiological association of hafnia alvei with diarrhea, because the organism was isolated from 12 of 77 (16%) adult finnish tourists to morocco who developed diarrhea and from 0 of 321 tourists without diarrhea (p < 0.001). from another group of 112 adult finnish diarrheal patients, only 2 (2%) yielded h. alvei. in contrast to some bangladeshi strains of h. alvei, the finnish strains were negative for the attachment-effacement lesion by an in vitro fluorescent acting staining test ...19947814573
[the heterogeneity of the antilysozyme activity in populations of opportunistic enterobacteria].1,484 clones of 104 strains representing 11 genera of the family enterobacteriaceae and having different levels of lysozyme activity were tested for the presence of this activity. in the populations of 53.9% of strains under study the heterogeneity of the levels of antilysozyme activity was noted. in 13.4% of strains under study clones having no antilysozyme activity were detected. among escherichia, hafnia and enterobacter strains without antilysozyme activity individual clones having such acti ...19947856344
3-deoxy-octulosonic-acid-containing hexasaccharide fragment of unusual core type isolated from hafnia alvei 2 lipopolysaccharide.the hexasaccharide containing 3-deoxy-octulosonic acid (kdo) was isolated from the carbohydrate material obtained after the mild acid hydrolysis of lipopolysaccharide of hafnia alvei strain 2. the hexasaccharide was purified by gel filtration on bio-gel p-4 and p-2 columns. on the basis of sugar and methylation analyses, one- and two-dimensional nmr spectroscopic methods, the hexasaccharide was identified as [formula: see text] the tetrasaccharide linked to position 7 of kdo is also part of the ...19957867651
comparison of the antibody responses to the 77 klebsiella capsular types in ankylosing spondylitis and various rheumatic diseases.the production of antibodies to klebsiella capsular polysaccharides was measured in sera from either hla-b27-positive (hla-b27+) or hla-b27-negative (hla-b27-) patients with classical ankylosing spondylitis (n = 54). these sera were compared with sera from patients with various rheumatic diseases (n = 82) and hla-b27+ or hla-b27- healthy individuals (n = 85). all sera were analyzed by means of an enzyme-linked immunosorbent assay specific to each of the 77 klebsiella serotypes. the sera from hla ...19947927763
unique fimbriae-like structures encoded by sefd of the sef14 fimbrial gene cluster of salmonella enteritidis.the sef14 gene cluster of salmonella enteritidis was recently shown to contain three genes, sefabc, encoding a unique fimbrin, and proteins homologous to fimbrial chaperones and outer membrane proteins (ushers), respectively. a fourth open reading frame, designated sefd, was found immediately downstream of sefabc and overlapping sefc. the translated protein sequence of sefd was unique, but the composition was similar to that of other bacterial fimbriae. sefd was produced in abundance by wild-typ ...19947934897
a novel strategy for the isolation of luxi homologues: evidence for the widespread distribution of a luxr:luxi superfamily in enteric bacteria.the pheromone n-(3-oxohexanoyl)-l-homoserine lactone (ohhl) regulates expression of bioluminescence in the marine bacterium vibrio fischeri, the production of carbapenem antibiotic in erwinia carotovora and exoenzymes in both e. carotovora and pseudomonas aeruginosa. a characteristic feature of this regulatory mechanism in v. fischeri is that it is cell density-dependent, reflecting the need to accumulate sufficient pheromone to trigger the induction of gene expression. using a lux plasmid-based ...19937968529
[hafnia alvei isolated in acute gastroenteritis]. 19947996884
reactive arthritis associated with hafnia alvei enteritis. 19948003071
[the detection of antagonistic strains in representatives of the genera bacillus and hafnia]. 19938079538
phenotypic diversity of anaerobic glycerol dissimilation shown by seven enterobacterial species.the anaerobic glycerol pathway was studied in seven enterobacterial species selected as representative of different behaviours in terms of anaerobic glycerol dissimilation. the presence of oxidative and reductive pathways of the dha regulon in klebsiella pneumoniae enabled the cells to grow fermentatively on glycerol. the first two enzymes of the dha regulon (glycerol dehydrogenase type i and dihydroxyacetone kinase) represent the oxidative branch, while the latter two (glycerol dehydratase and ...19948090993
serological and structural features of hafnia alvei lipopolysaccharides containing d-3-hydroxybutyric acid.the serological heterogeneity of hafnia alvei lipopolysaccharides from strains atcc 13337, 1187, 1221, 114/60, 1211 and 1216, that contain d-3-hydroxybutyric acid, was analyzed by rocket immunoelectrophoresis, immunoblotting and passive hemagglutination. the significance of d-3-hydroxybutyric acid component for their cross-reactivity has been discussed. the results obtained allowed us to place four h. alvei strains (atcc 13337, 1187, 1221 and 114/60) in one serotype (a) and to consider two other ...19948156055
multipoint identification of enterobacteriaceae: report of the british society for microbial technology collaborative study.to evaluate the accuracy and reproducibility of multipoint identification schemes in a multicentre trial.19938157751
hafnia alvei. respiratory tract isolates in a community hospital over a three-year period and a literature review.in a retrospective review, a group of seven patients were found to have a sputum culture positive for hafnia alvei. hafnia alvei is a gram-negative enteric and oropharyngeal bacillus and usually is nonpathogenic. all our patients had a chronic underlying illness and one of the patients was endotracheally intubated at the time of the isolation of this organism. six of seven patients had other organisms isolated along with h alvei, and only one patient had a pure growth of h alvei confirmed by a c ...19948162732
characterization of the c-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic escherichia coli, citrobacter freundii, and hafnia alvei.surface proteins called intimins (int), which are homologous to the invasin protein (inv) of yersinia spp., play a role in inducing brush border damage, termed attachment and effacement, which follows infection by enteropathogenic and enterohemorrhagic escherichia coli, citrobacter freundii biotype 4280, and hafnia alvei. maltose-binding protein (mbp) fusions containing the c-terminal 280 amino acids of int-like proteins of strains of enteropathogenic e. coli, enterohemorrhagic e. coli, h. alvei ...19948168946
[studies of humoral immunity induced by proteins of the cell wall of hafnia alvei].it was found that cell wall proteins (cwp) of hafnia alvei applied intraperitoneally to mice are inducing, beside previously detected cellular immunity, also humoral immunity. applying elisa immunoenzymatic test in which a conjugate enabling demonstration of igg antibodies was applied, it was found that cwp of hafnia alvei induce appearance of specific antibodies in a high titer, but their presence in mouse serum is short and independent from applied doses of cwp. humoral immunity induced by cwp ...19938189802
study of enterobacteriaceae throughout the manufacturing and ripening of hard goats' cheese.the evolution of the counts and the species of enterobacteriaceae as well as some physico-chemical parameters (ph, aw and nacl and moisture contents) during manufacturing and ripening of a hard spanish goats' cheese of the armada-sobado variety were studied. enterobacteriaceae (mean log counts 4.45 g-1 in milk) increased 0.71-2.18 log units in curd and afterwards decreased until they disappeared after 2-4 weeks of ripening. this premature disappearance seems to be due to the decrease in aw value ...19938244902
ceftazidime resistance in hafnia alvei.two morphotypes of hafnia alvei differed in their susceptibilities to beta-lactam antibiotics. both produced an inducible bush group 1 beta-lactamase. hyperinducibility of this enzyme was associated with reduced susceptibility in one morphotype.19938328790
a multidrug resistance regulatory chromosomal locus is widespread among enteric bacteria.constitutive expression of the mar operon (marrab) in escherichia coli produces a multiple antibiotic resistance phenotype mediated by the expression of multiple genetic loci in response to regulatory proteins in the operon. a mar-specific dna probe was used to search for the operon in bacterial strains representing 53 species and 27 genera. among these, 6 other enterobacteriaceae, salmonella, shigella, klebsiella, citrobacter, hafnia, and enterobacter species, contained dna sequences that hybri ...19938335992
nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of escherichia coli.arginine decarboxylase (encoded by adi) is induced under conditions of acidic ph, anaerobiosis, and rich medium. the dna sequence of a 3-kb fragment of the escherichia coli chromosome encoding biodegradative arginine decarboxylase was determined. this sequence encodes a protein of 755 amino acids with a molecular size of 84,420 daltons. the molecular weight and predicted adi amino acid composition agree with those found in earlier work. the amino acid sequence of arginine decarboxylase showed ho ...19938383109
analysis of 7-substituted sialic acid in some enterobacterial lipopolysaccharides.sialic acid-containing lipopolysaccharides (lps) were isolated from six bacterial strains of the family enterobacteriaceae. sialic acid was released from permethylated lps by methanolysis, and partially o-methylated n-acetyl-n-methyl-neuraminic acid methyl ester methyl glycosides were analyzed by gas-liquid chromatography-electron ionization mass spectrometry. it was proved that all lps contain n-acetylneuraminic acid (neuac). the occurrence of 7-substituted neuac in escherichia coli serotypes o ...19938444811
acute gastroenteritis caused by hafnia alvei in children. 19938452958
structure of the o-specific polysaccharide containing pentitol phosphate, isolated from hafnia alvei strain pcm 1191 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide produced by hafnia alvei strain pcm 1191 was shown by composition and methylation analyses, periodate oxidation and one- and two-dimensional 1h- and 31p-nmr spectroscopy to be a polymer of a branched hexasaccharide repeating units having the structure: [formula: see text] where laraol = l-arabitol the polysaccharide of 1191 strain has teichoic-acid-like character. its peculiar feature is the presence of arabitol phosphate, a component obser ...19938504816
structure of the hafnia alvei strain pcm 1188 o-specific polysaccharide.the lipopolysaccharide was extracted from cells of hafnia alvei pcm 1188 strain and, after mild acid hydrolysis, the o-specific polysaccharide isolated and characterized. on the basis of sugar and methylation analysis, fab mass spectrometry and nmr spectroscopy of the polysaccharide and oligosaccharides obtained after smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the o-specific polysaccharide was shown to be the pentasaccharide: [formula: see text]19958556734
[gram-negative bacteria contaminating the process of producing lysine].the authors have isolated gram-negative bacteria of the enterobacteriaceae family from the culture liquid of industrial fermenters with low yield of lysine. most of them possessed the characters typical of klebsiella pneumoniae and escherichia coli, the rest were identified as the representatives of genera proteus, providencia, enterobacter, hafnia. strains of klebsiella pneumoniae, e.coli, proteus rettgeri manifested lysine-decarboxylase activity. the capacity of some strains to destruct lysine ...19958563944
structure of the o-specific polysaccharide of hafnia alvei 1204 containing 3,6-dideoxy-3-formamido-d-glucose.the o-specific polysaccharide of hafnia alvei strain 1204 has a hexasaccharide repeating unit containing d-mannose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, and 3,6-dideoxy-3-formamido-d-glucose (qui3nfo) in the ratios 2:1:1:1:1 as well as o-acetyl groups. on the basis of methylation analysis of the intact, carboxyl-reduced, and smith-degraded polysaccharide as well as 1d and 2d nmr spectroscopy, including 1d total correlation spectroscopy, 1d noe spectr ...19958565006
structural studies of the o-specific polysaccharide of hafnia alvei strain 1209 lipopolysaccharide.the structure of the o-specific side chains of the hafnia alvei strain 1209 lipopolysaccharide has been investigated. methylation analysis and 1h-nmr and 13c-nmr spectroscopy were the principal methods used. it is concluded that the polysaccharide is composed of pentasaccharide repeating units that have the following structure: -->3)-beta-d-galp-(1-->4)-alpha-d-glcp-(1-->4)-beta-d-glepa-(1--> 3)-beta-d-galpnac-(1 --> 4 increases 1 alpha-l-rhap the relative intensity of the signals from the termi ...19968647107
the occurrence of glycine in bacterial lipopolysaccharides.the aminoacyl analysis of endotoxic lipopolysaccharides (lps) isolated from several bacteria revealed essential amounts of glycine, among the inherent lps components. significant amounts of the glycine was detected in lipopolysaccharides isolated from over 30 strains of escherichia, salmonella, hafnia, citrobacter and shigella species. glycine as a single amino acid was found only in a core part of lps. molar ratio of glycine in core oligosaccharide fraction ranged from 0.2 to 0.6 per 3 heptoses ...19968739188
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...19968742354
structure of the o-specific polysaccharide, containing a glycerol phosphate substituent, of hafnia alvei strain 1220 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide produced by hafnia alvei strain 1220 contained d-glucose, d-galactose, n-acetyl-d-glucosamine, n-acetyl-l-fucosamine (2-acetamido-2,6-dideoxy-l-galactose), glycerol, and phosphate. it was proved by composition and methylation analyses, smith degradation, dephosphorylation, and one- and two-dimensional 1h nmr spectroscopy to be a teichoic acid-like polymer with a branched hexasaccharide repeating unit of the following structure. [sequence: s ...19968765061
clinical significance of extraintestinal hafnia alvei isolates from 61 patients and review of the literature.hafnia alvei is a gram-negative bacterium that is rarely isolated from human specimens and is rarely considered to be pathogenic. it has been associated with gastroenteritis, meningitis, bacteremia, pneumonia, nosocomial wound infections, endophthalmitis, and a buttock abscess. we studied 80 h. alvei isolates recovered from 61 patients within a period of 30 months. h. alvei was cultured from sites that included the respiratory tract (n = 38), the gastrointestinal tract (n = 16), and the urogenit ...19968783707
the sensitivity of hafnia alvei strains to the bactericidal effect of serum.most hafnia alvei strains are sensitive to the bactericidal action of normal bovine serum (nbs) as well as to a serum in which the alternative pathway of complement activation has been thermally blocked. introduction of polysaccharides (ps) to nbs lowers the bactericidal effect. in a serum in which the alternative pathway of complement activation is blocked, ps completely cancels the bacterial effect.19968821399
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