TitleAbstractYear(sorted descending)
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
bacteriological quality of aquacultured freshwater fish portions in prepackaged trays stored at 3 degrees c.fresh trout fillets and salmon slices packed in trays were obtained from two multinational chain supermarkets and evaluated for freshness and bacteriological quality immediately after packaging and during storage at 3 degrees c. initial aerobic counts at 30 and 25 degrees c were significantly (p < 0.05) lower in trout fillets (5.27 +/- 0.57 and 4.87 +/- 0.80 log cfu/g, respectively) than in salmon slices, where levels in excess of 6 log cfu/g were found. in both products, initial enterobacteriac ...200111563518
lactate dehydrogenase release assay from vero cells to distinguish verotoxin producing escherichia coli from non-verotoxin producing strains.the vero cell assay presently used for virulence testing of verotoxigenic escherichia coli (vtec) requires at least 48-96 h where cytotoxicity effects are examined under a microscope. here, a complimentary rapid assay was developed that measures endogenous lactate dehydrogenase (ldh) release from vero or hep-2 cells as an indicator of cytotoxicity. toxin preparations from 24 vtec strains induced 36-89% ldh from vero cells and 15-62% ldh from hep-2 cells in 12-16 h. a verotoxin-positive but enter ...200111118652
host and geographical factors influence the thermal niche of enteric bacteria isolated from native australian mammals.the thermal profiles of 118 bacterial strains, representing six species of the family enterobacteriaceae, isolated from a variety of native australian mammals were determined under in vitro conditions. each of the bacterial species had a unique thermal profile and differed in their minimum or maximum temperature for growth and in their response to changing temperatures. the taxonomic classification of the host from which the bacterial strains were isolated explained a significant amount of the v ...200111742550
the eaea gene is not found in hafnia alvei from patients with diarrhea in aragón, spain.a total of 102 hafnia alvei clinical strains isolated from different patients with diarrhea has been tested, using polymerase chain reaction and dot-blot hybridization, for the enteropathogenic escherichia coli attaching and effacing a (eaea) gene to establish their role as a causative agent of diarrhea in our environment. none of them was positive for the eaea gene. we cannot consider the eaea gene as the virulence-associated factor implicated in the h. alvei strains isolated from diarrheal fec ...200111770828
damage threshold prediction of hafnia-silica multilayer coatings by nondestructive evaluation of fluence-limiting defects.a variety of microscopic techniques were employed to characterize fluence-limiting defects in hafnia-silica multilayer coatings manufactured for the national ignition facility, a fusion laser with a wavelength of 1.053 mum and a pulse width of 3 ns. photothermal microscopy, with the surface thermal lens effect, was used to map the absorption and thermal characteristics of 3 mm x 3 mm areas of the coatings. high-resolution subaperture scans, with a 1-mum step size and a 3-mum pump-beam diameter, ...200118357189
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
a note on escherichia coli o157:h7 serotype in turkish meat products.255 minced beef, 101 soudjouk and 50 uncooked hamburger samples were analyzed for the presence of escherichia coli o157:h7 serotype. m-ec and lst broths were used as selective enrichment media and smac agar was used as a selective isolation medium. a total of 3 e. coli o157 were isolated by conventional culture techniques; one from each of minced beef, uncooked hamburger and soudjouk but none were identified as the h7 serotype. for determination of selective media-growing cohabitant bacteria, 26 ...200022062162
the effect of biogenic amine production by single bacterial cultures and metabiosis on cold-smoked salmon.biogenic amines are important indicators of spoilage in vacuum-packed cold-smoked salmon. it is the aim of this study to identify bacteria responsible for biogenic amine production in cold-smoked salmon.200011123465
[diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis].a new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over endo medium in its differentiating and inhibiting properties. this medium permitted the easy differentiation of yersinia pseudotuberculosis from y. enterocolitica, as well as from escherichia coli, shigella flexneri, klebsiella pneumoniae, k. rhinoscleromatis, hafnia, enterobacter and citrobacter by color; from proteus inconstans by swarming. ...200011210625
topical antibiotics on tracheostoma prevents exogenous colonization and infection of lower airways in children.patients requiring long-term ventilation are at high risk of lower airway infections, generally of endogenous development. patients on long-term ventilation, in particular via a tracheostomy, may develop tracheobronchitis or pneumonia of exogenous pathogenesis, ie, caused by microorganisms not carried in the oropharynx. the frequency of exogenous colonization or infection has previously been reported to be as high as 33%. a prospective observational cohort study of 2 years was undertaken to eval ...200010669698
immunochemical aspects of hafnia alvei o the article the composition and structure of the o-specific polysaccharide chains and core region of the o antigens isolated from over 20 h. alvei strains is overviewed. moreover, the correlation between the structure and immunospecificity of the o antigens is presented and discussed.200010683466
hb ube-2 [alpha68(e17)asn-asp] and hb hafnia [beta116(g18)his-->gln] observed during neonatal screening in brussels. 200010722118
[empyema due to hafnia alvei]. 200010736806
changes in protein composition and hydrolytic enzyme activity of escherichia coli and hafnia alvei grown in human fluids.growing of escherichia coli and hafnia alvei cells in several cell-free human fluids, such as normal serum, serum from diabetic patients, pleural, ascitic and spinal fluid, revealed that various biochemical changes occurred. protein profile on sds-page as well as acid and alkaline phosphohydrolytic enzymes on native gels of cell extracts were affected after culturing of bacteria in the above fluids. gelatinolytic and hyaluronolytic activity was of interest because both of them are histolytic enz ...200010772159
biochemical-genetic characterization and regulation of expression of an acc-1-like chromosome-borne cephalosporinase from hafnia alvei.a naturally occurring ampc beta-lactamase (cephalosporinase) gene was cloned from the hafnia alvei 1 clinical isolate and expressed in escherichia coli. the deduced ampc beta-lactamase (acc-2) had a pi of 8 and a relative molecular mass of 37 kda and showed 50 and 47% amino acid identity with the chromosome-encoded ampcs from serratia marcescens and providentia stuartii, respectively. it had 94% amino acid identity with the recently described plasmid-borne cephalosporinase acc-1 from klebsiella ...200010817695
outbreak of klebsiella pneumoniae producing transferable ampc-type beta-lactamase (acc-1) originating from hafnia alvei.fifty-two strains of klebsiella pneumoniae producing an ampc-type plasmid-mediated beta-lactamase were isolated from 13 patients in the same intensive care unit between march 1998 and february 1999. these strains were resistant to ceftazidime, cefotaxime and ceftriaxone, but susceptible to cefoxitin, cefepime and aztreonam. plasmid content and genomic dna restriction pattern analysis suggested dissemination of a single clone. two beta-lactamases were identified, tem-1 and acc-1. we used internal ...200010828397
the capacity of enterobacteriaceae species to produce biogenic amines in cheese.the amino acid decarboxylating activity and production of biogenic amines by 104 cheese-associated enterobacteriaceae species (58 enterobacter, 18 serratia, eight escherichia, seven hafnia, six arizona, four citrobacter and three klebsiella) were investigated. all strains could decarboxylate at least two amino acids in möller's broth and in niven's medium, and the decarboxylase activity was strain specific. in a laboratory medium containing all free amino acids, all strains could produce more th ...200010972722
soybean vegetable protein (tofu) preserved with high pressure.tofu is a soybean vegetable protein that asians have long consumed; its intake is increasing in other countries. tofu was purchased at a local shop. the tofu samples were already preserved in plastic bags subjected to vacuum and storage (5 c). tofu samples were subjected to high pressure (hp) of 400 mpa at 5 c for 5, 30, and 45 mm. microbial analysis, sensorial evaluation, and structure were determined. hp treatment in tofu reduces the microbial population. most of the microorganisms found in th ...200011032484
heterogeneity of ampc cephalosporinases of hafnia alvei clinical isolates expressing inducible or constitutive ceftazidime resistance phenotypes.ten unrelated hafnia alvei clinical isolates were grouped according to either their low-level and inducible cephalosporinase production or their high-level and constitutive cephalosporinase production phenotype. their ampc sequences shared 85 to 100% amino acid identity. the immediate genetic environment of ampc genes was conserved in h. alvei isolates but was different from that found in other ampc-possessing enterobacterial species.200011036059
extraintestinal infection due to hafnia alvei.the aim of this study was to establish the clinical features of extraintestinal infections caused by hafnia alvei. over a 5-year period (1994-1998), data were collected regarding inpatients (n = 8) with nosocomial (n = 5) or community-acquired (n = 3) infections caused by hafnia alvei. the mean age of the patients was 47 +/- 21 years. three patients had hospital-acquired urinary tract infections. hafnia alvei also caused community-acquired cholangitis, cholecystitis, appendicitis, psoas abscess ...200011057506
structural and serological studies of the related o-specific polysaccharides of proteus vulgaris o21 and proteus mirabilis o48 having oligosaccharide-phosphate repeating units.the o-specific polysaccharide chains (o-antigens) of the lipopolysaccharides (lpss) of proteus mirabilis o48 and proteus vulgaris o21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. polysaccharides and an oligosaccharide were derived from the lpss by various degradation procedures and studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c and 1h,31p hmqc experiments. the following rel ...200011082201
structure of the lipopolysaccharide core region of hafnia alvei strains 1185 and 1204.sugar and methylation analyses using gas chromatography/mass spectrometry and nmr spectroscopy proved that the core oligosaccharides of hafnia alvei strains 1185 and 1204 have the following formula: carbohydrate sequence [see text] where kdo = 3-deoxy-oct-2-ulosonic acid and p-petn = diphosphorylethanolamine. the structure shown above is a slight modification of the typical core region of h. alvei lipopolysaccharides. the difference refers to one sugar only: terminal galactose is present in the ...200011086705
[hafnia alvei septicemia revealing pyocholecystitis complicated by liver abscess in an immunocompetent patient]. 200011094615
in vitro activity of gemifloxacin (sb-265805) compared with 14 other antimicrobials against intestinal pathogens.we studied the in vitro activity of gemifloxacin (sb-265805) and 14 comparator antimicrobials against 288 recent isolates of enteropathogenic bacteria (106 salmonella: spp., 32 hafnia alvei, 22 yersinia enterocolitica, 21 shigella: spp., 16 aeromonas: spp. and 91 campylobacter jejuni). gemifloxacin, the other fluoroquinolones and cefotaxime were very active against all microorganisms tested except for c. jejuni. seventy-seven per cent of isolates of c. jejuni were inhibited by erythromycin < or ...200011102426
[mitral valve prosthetic endocarditis due to hafnia alvei]. 199910091216
important aspects of the colonization of central venous catheter.this study comprises five different kind of venous central catheters, 103 in total, made of polyurethane tecoflex, polyurethane vialon, ptfe and pvc, and the influence of their raw material on the microbial colonization. patients age and sex, besides their clinical conditions, were taken into account, and neither considered as a sample vicious, nor associated with colonization. when the tips of the catheters were asseptically inoculated in tryptic soy broth and tioglicolate, colonization was det ...199910326311
non-typical lipopolysaccharide core regions of some hafnia alvei strains: structural and serological studies.the lipopolysaccharides of hafnia alvei strains 23, 1222 and 39 were found to have non-typical core region. on the basis of sugar and methylation analyses, 1h-nuclear magnetic resonance spectra and matrix-assisted laser-desorption ionization-time of flight mass spectrometry, it was concluded that the core oligosaccharide of strains 23 and 1222 has the same structure as escherichia coli r4 core region, and the core oligosaccharide of strain 39 has the structure of salmonella ra core. using the se ...199910340714
prototypal diarrheagenic strains of hafnia alvei are actually members of the genus escherichia.we analyzed five bacterial strains, designated 19982, 9194, 10457, 10790, and 12502, that were isolated from stool specimens of individuals with diarrheal illness by the international centre for diarrhoeal disease research in dhaka, bangladesh (m. j. albert, s. m. faruque, m. ansaruzzaman, m. m. islam, k. haider, k. alam, i. kabir, and r. robins-browne, j. med. microbiol. 37:310-314, 1992). the strains were initially identified as hafnia alvei with a commercial identification system and were rep ...199910405374
the distribution of enteric bacteria from australian mammals: host and geographical effects.bacteria of the family enterobacteriaceae were isolated from 642 mammalian hosts, representing 16 families and 79 species, collected from throughout australia. escherichia coli was the most common of the 24 enteric species recovered and represented almost half of the isolates. association analysis revealed that most other species of bacteria were less likely to be recovered from hosts in which e. coli was present. the composition of the enteric community of a host was found to be determined by b ...199910537188
the genetic structure of enteric bacteria from australian mammals.a total of 246 isolates representing five species of the family enterobacteriaceae, taken from a variety of australian mammal species, were characterized using multi-locus enzyme electrophoresis. genome diversity estimates varied significantly among species, with the klebsiella pneumoniae sample exhibiting the lowest diversity and the citrobacter freundii sample the highest. multi-locus linkage disequilibrium estimates revealed that alleles were non-randomly associated in all five species sample ...199910537189
structural studies of the o-specific polysaccharide of hafnia alvei strain pcm 1207 lipopolysaccharide.the structure of the o-specific side-chain of the hafnia alvei strain pcm 1207 lipopolysaccharide (lps) has been investigated. methylation analysis, partial acid hydrolysis, matrix-assisted laser-desorption ionization time-of-flight (maldi-tof) ms, fast atom bombardment (fab)-ms/ms and 1h- and 13c-nmr spectroscopy were the principal methods used. glycerol phosphate was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was establishe ...199910542050
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
identification of hafnia alvei with the microscan walkaway system.hafnia alvei is a gram-negative facultatively anaerobic bacillus that belongs to the family enterobacteriaceae. this organism is a causative agent of intestinal disorders and is found in different environments. h. alvei has received increased clinical attention as a cause of different infections in humans. this study was performed to compare the microscan walkaway automated identification system in conjunction with the new microscan combo negative type 1s panels with conventional biochemical met ...199910565961
characterization of very dense mineral oxide-gel composites for fluidized-bed adsorption of biomolecules.efficient design of fluidized-bed biomolecule adsorption from crude feed stock requires particles with elevated density, large adsorption capacity and broad chemical stability. moreover, combinations of small particle diameters with high densities allow for high fluidization velocities while preserving a rapid mass transfer. this approach has been implemented by combining stable porous mineral oxide of high density (2.2, 4.7, 5.7, 9.4 g/ml) with functionalized hydrogels. the cross-linked hydroge ...199910734563
a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe. 199811864254
hafnium germanate from a hydrous hafnium germanium oxide gel.the gel chemistry of germanium is explored through the formation and composition of a hydrous metal oxide precursor gel used in the preparation of the hfgeo(4) and hfgeo(4):ti x-ray phosphors. the enhanced solubility of hexagonal geo(2) in dilute ammoniacal solutions is exploited to give a convenient and high-yield precipitation. the precursor gel is shown by ft-ir to be a diphasic mixture of hydrous hafnia and an ammonium germanate gel. thermal treatment drives the crystallization of a hafnium- ...199811670345
structural heterogeneity of the sialic-acid-containing oligosaccharides from the lipopolysaccharide of hafnia alvei strain 2 as detected by fabms studies.the structure of four oligosaccharide fractions from the hafnia alvei strain 2 lipopolysaccharide (lps) have been assigned by fabms. this approach corroborates data previously established by nmr spectroscopy for the major oligosaccharides in these fractions [a. gamian, e. romanowska, u. dabrowski, j. dabrowski, biochemistry 30 (1991) 5032-5038; e. katzenellenbogen, a. gamian, e. romanowska, u. dabrowski, j. dabrowski, biochem. biophys. res. commun. 194 (1993) 1058-1064; n. ravenscroft, a. gamian ...199810335589
immunochemical studies of the lipopolysaccharide o-specific polysaccharide of hafnia alvei pcm 1199 related to h. alvei pcm 1205.on the basis of chemical analyses and nmr spectroscopic studies, it was found that the o-specific polysaccharide (o-ps) isolated from the hafnia alvei pcm 1199 lipopolysaccharide (lps) is a glycerol teichoic-acid-like polymer having a repeating unit of the following structure: [structure in text] where qui4nac is 4-acetamido-4,6-dideoxyglucose and o-acetylation at both positions is non-stoichiometric. the glycosidic linkage of the lateral beta-d-glcpnac residue is acid-labile and cleaved from th ...19989490075
amino acid decarboxylase capability of microorganisms isolated in spanish fermented meat products.enterobacteria, lactic acid bacteria (lab) and gram-positive cocci were isolated from spanish meat products. the most frequent species in the meat products studied were identified as lactobacillus sake, lactobacillus plantarum and lactobacillus curvatus from de man-rogosa-sharpe agar; staphylococcus xylosus, staphylococcus saprophyticus and micrococcus varians from mannitol salt phenol-red agar; and hafnia alvei, escherichia coli, pseudomonas fluorescens, enterobacter amnigenes and enterobacter ...19989553801
enterobacteriaceae found in high numbers in fish, minced meat and pasteurised milk or cream and the presence of toxin encoding genes.enterobacteriaceae were found in high numbers after storage at 7 degrees c in 6% of consumers packs of pasteurised milk or cream, in 31% of retailed fish and in 100% of retail packs of minced meat. seventy two fresh-water fishes, 40 packs of minced meat and 430 milk packs were sampled. one hundred and eighty four isolates were randomly picked from tryptone glucose extract (tge) agar (30 degrees c for 3d) or violet red bile glucose (vrbg) agar (37 degrees c for 1d). in minced meat, serratia lique ...19989562874
pulsed-field gel electrophoresis typing of hafnia alvei isolated from chub-packed and retail ground beef.thirty-eight isolates of hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed-field gel electrophoresis (pfge) patterns. the isolates were recovered from chub-packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). biochemical profiling differentiated the 38 isolates into five groups and a commercial ribotyping method recognized 11 groups, whereas pfge differentiated the same 38 isolates into 19 groups. these ...19989569690
[hafnia alvei in acute gastroenteritis in children]. 19989608106
espe, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kda protein.shiga toxin-producing escherichia coli (stec), enteropathogenic e. coli (epec) and some strains of hafnia alvei are capable of inducing attaching and effacing (a/e) lesions, characterized by tight apposition of the bacteria to the eukaryotic membrane and formation of actin-based pedestals. in this study, we report on the identification of espe, a novel secreted 80 kda protein of a/e bacteria. during infection, espe is delivered into the cytoplasm of the infected host cell, where it is detected a ...19989632251
reinvestigation of the o-specific polysaccharides of hafnia alvei lipopolysaccharides isolated from strains atcc 13337 and 1187.the structure of the o-specific polysaccharides of the lipopolysaccharides produced by hafnia alvei strains atcc 13337 and 1187 was reinvestigated. the position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1h detected, 31p edited nmr spectra. according to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: [formula: see text] where acyl = d-3-hydroxylbutyryl, and 3-o-ace ...19989658571
thermal death times of hafnia alvei cells in a model suspension and in artificially contaminated hot-smoked kahawai (arripis trutta).in new zealand the product most frequently implicated in cases of scombroid poisoning is hot-smoked kahawai (arripis trutta). using a hafnia alvei strain, previously isolated from a portion of hot-smoked kahawai with a histamine level of 1,659.4 mg/kg, thermal death trials were carried out in a model suspension (0.1% peptone) at 54, 55, 56, 57, and 58 degrees c. from the linear regression line (r2 = 0.98) fitted to observed d values plotted against temperature, calculated d values for 54, 55, 56 ...19989713770
[the characteristics of the causative agents of suppurative-inflammatory complications in hemophiliacs].morphological-cultural and physiological-biochemical properties of 24 strains of microorganisms agents of pyo-inflammatory complications of different localization in patients with hemophilia have been studied. microorganisms strains presented by the following species: staphylococcus aureus, s. epidermidis, s. saprophyticus, proteus vulgaris, p. morganii, hafnia alvei, serratia marcescens, have been identified. it was found out that in monoculture staphylococci prove to be the leading etiological ...19989859645
an homogeneous fluorescence polymerase chain reaction assay to identify salmonella.we have developed a semiquantitative homogeneous fluorescence assay that combines polymerase chain reaction (pcr) amplification with direct fluorescence detection (hf-pcr). the assay eliminates the need to perform gel electrophoresis on test samples. using a set of salmonella-specific primers, this system was used to verify suspect colonies from culture plates as salmonella. the fluorescence signal is generated by a nucleic acid dye, yo-pro-1, that is included in the amplification reaction. this ...19979056213
prevalence and mechanism of resistance to 'third-generation' cephalosporins in clinically relevant isolates of enterobacteriaceae from 43 hospitals in the uk, a uk survey of the occurrence of extended spectrum beta-lactamases, 96 hospitals submitted a total of 3951 non-selected, non-duplicate isolates of enterobacteriaceae from 100 patients in each hospital, 206 of these cultures being mixed and, therefore, discarded. these isolates were initially screened for strains likely to produce extended-spectrum beta-lactamases (esbls) by mic determination of beta-lactams followed by a bioassay, then disc approximation test and isoelectric focusing (ief). i ...19979069538
structural studies of the o-specific chain of hafnia alvei strain pcm 1190 lipopolysaccharide.the structure of the o-specific side-chain of the lipopolysaccharide of hafnia alvei strain pcm 1190 has been investigated. methylation analysis, partial acid hydrolysis, smith degradation, nmr spectroscopy, maldi-tof, and fab mass spectrometry in combination with collision-induced-decomposition ms/ms were the principal methods used. it was concluded that the polysaccharide is composed of heptasaccharide repeating units having the following structure: [formula: see text] only 80% of the repeatin ...19979090816
minimum growth temperatures of hafnia alvei and other enterobacteriaceae isolated from refrigerated meat determined with a temperature gradient incubator.minimum growth temperatures of hafnia alvei (n = 156) and other enterobacteriaceae isolates (n = 162) from refrigerated meat samples (n = 88) and control strains of h. alvei (n = 81) from clinical and environmental samples were determined with a plate-type continuous temperature gradient incubator on nutrient agar. the dominant species, hafnia alvei and serratia liquefaciens had mean minimum growth temperatures of 2.6 (range, 0.2-3.7 degrees c) and 1.7 (range, 0.2-2.6 degrees c), respectively. v ...19979105940
community-acquired hafnia alvei infection. 19979114222
structural studies of the o-specific polysaccharide of hafnia alvei strain pcm 1206 lipopolysaccharide containing d-allothreonine.the structure of the o-specific side-chain of the hafnia alvei strain pcm 1206 lipopolysaccharide has been investigated. methylation analysis, partial acid hydrolysis, fab-ms/ms and 1h-nmr and 13c-nmr spectroscopy were the principal methods used. d-allothreonine (d-athr), amide-linked to the d-galacturonic acid, was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established: [structure: see text].19979119027
characteristics of alcohol dehydrogenases of certain aerobic bacteria representing human colonic flora.we have recently proposed the existence of a bacteriocolonic pathway for ethanol oxidation [i.e., ethanol is oxidized by alcohol dehydrogenases (adhs) of intestinal bacteria resulting in high intracolonic levels of reactive and toxic acetaldehyde]. the aim of this in vitro study was to characterize further adh activity of some aerobic bacteria, representing the normal human colonic flora. these bacteria were earlier shown to possess high cytosolic adh activities (escherichia coli ih 133369, kleb ...19979161610
structural studies of the o-specific chains of hafnia alvei strains 744, pcm 1194 and pcm 1210 lipopolysaccharides.the structures of the o-specific chains of the hafnia alvei strain 744 and pcm strains 1194 and 1210 lipopolysaccharides have been investigated. methylation analysis, dephosphorylation, nmr spectroscopy, matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry and fast-atom-bombardment mass spectrometry were the principal methods used. it was concluded that the polysaccharides of strains 744 and pcm 1194 are composed of the same pentasaccharide repeating unit having the follo ...19979183004
hafnia alvei infection after liver transplantation. 19979195098
structure of the o-polysaccharide from the lps of a hafnia alvei strain isolated from a patient with suspect yersinosis.the carbohydrate backbone of the hafnia alvei strain y166/91 lipopolysaccharide (lps) was isolated by mild acid hydrolysis followed by gel permeation chromatography and studied by nmr spectroscopy and methylation analysis. treatment with periodate and hypoiodite gave a modified polysaccharide which was also characterised. it was concluded that the polysaccharide has the following structure with two distinct regions, which are connected. the chain length parameters m and n were not determined but ...19979203343
hafnia alvei septicemia in two patients with digestive malignancies. 19979238449
inhibition of microbial growth in chub-packed ground beef by refrigeration (2 degrees c) and medium-dose (2.2 to 2.4 kgy) irradiation.two studies were conducted to monitor microbial growth in chub-packed ground beef. in the first study, the effect of storage temperatures (2 and 7 degrees c) on chubs was examined for up to 18 days. storage of chubs at 2 degrees c slowed microbial growth such that total counts exceeded 7.5 log10 cfu/g within 10 days compared to within 4 days for chubs stored at 7 degrees c. microbial counts > or = 7.5 log10 cfu/g were associated with off-odors and samples were considered spoiled. the predominant ...19979310852
[hafnia alvei bacteremia in an aids patient]. 19979324603
the escherichia coli ldcc gene encodes another lysine decarboxylase, probably a constitutive enzyme.a gene (designated ldcc) mapped at 4.6 min on the escherichia coli chromosome codes for a protein of 713 amino acids (aa) that shows strong similarities in both size and amino-acid sequence (69% identical residues and 85% conserved residues) to lysine decarboxylase (ldc) from e. coli (cada, acid-inducible ldc, 715 aa) or from hafnia alvei (739 aa). a puc18 derivative carrying the ldcc gene conferred high ldc activities on an e. coli strain devoid of the functional cada gene, even when the bacter ...19979339543
inhibition of salmonella typhimurium attachment to chicken cecal mucus by intestinal isolates of enterobacteriaceae and lactobacilli.the ability of selected strains of enterobacteriaceae or lactobacilli isolated from the intestines of adult chickens to inhibit in vitro attachment of salmonella typhimurium 3333/o to cecal mucus in the presence or absence of d-mannose was determined. attachment in the absence of mannose was reduced by prior exposure of mucus to cultures of two isolates of enterobacteriaceae, an escherichia coli and a hafnia alvei strain, but not to a third isolate, an enterobacter agglomerans strain. attachment ...19979356699
septicemia associated with hafnia alvei in laying the present work hafnia alvei was isolated from laying hens displaying a reduction in egg production, loss of appetite, opisthotonus, and death. multifocal necrotizing hepatitis and splenitis were the most prominent lesions. the organism was identified microbiologically. laying hens were experimentally inoculated by the oral and intraperitoneal route to show the pathogenicity of the organism. a very similar clinicopathologic effect resulted from this trial. several experimentally infected lay ...19979356725
electrochemical measurement of trace concentrations of biological hydrogen produced by enterobacteriaceae.accurate measurements of the hydrogen gas produced by escherichia coli and hafnia alvei pure cultures during glucose metabolism were performed under different growth conditions: stagnant, with magnetic stirring or with vibrational shaking. these measurements were carried out using an electrochemical hydrogen sensor based on a platinum-coated solid polymer electrolyte membrane (pt-spe). the results obtained were dependent on the hydrodynamic conditions of the growth, with greater hydrogen product ...19979404509
l-ornithine decarboxylase from hafnia alvei has a novel l-ornithine oxidase activity.a novel activity producing gamma-aminobutyric acid (gaba) from l-ornithine in the presence of nad(p)+ was found in the crude extract of l-ornithine-induced hafnia alvei, in addition to l-ornithine decarboxylase (odc) activity. the reaction system for the former activity consisted of two enzymes, l-ornithine oxidase (decarboxylating, ood) and gamma-aminobutyraldehyde (gabl) dehydrogenase (gdh). ood catalyzed the conversion of l-ornithine into gabl, co2, nh3, and h2o2 in the presence of o2, and gd ...19979443811
nmr spectroscopic elucidation of the structure of a glycerol teichoic acid-like o-specific polysaccharide of the bacterium hafnia alvei strain pcm 1199.the structure of the acidic o-specific polysaccharide of a gram-negative bacterium, h. alvei strain pcm 1199, was studied by nmr spectroscopy including two-dimensional correlation spectroscopy (cosy), total correlation spectroscopy (tocsy), nuclear overhauser effect spectroscopy (noesy), 1h, 13c heteronuclear single-quantum coherence (hsqc), 1h, 13c heteronuclear multiple-bond correlation (hmbc), and one-dimensional 1h, 31p heteronuclear multiple-quantum coherence (hmqc) experiments. it was foun ...19979481879
[structural studies of carbohydrate lipopolysaccharide sections in hafnia alvei].the structures of 14 o-specific polysaccharides isolated from hafnia alvei lipopolysaccharides have been described. the structures of the common core existing in most hafnia alvei strains examined so far and the core-like trisaccharide found in some hafnia alvei strains have been also presented.19969072760
[lipopolysaccharides containing sialic acid].sialic acids which are important constituents of animal tissue glycoconjugates are also present in antigens of some bacterial strains. capsular polysaccharides with sialic acid have been extensively studied whereas little is known on lipopolysaccharides which contain sialic acid. the paper presents review of the data concerning structure of bacterial endotoxic lipopolysaccharides. methodological peculiarities were described. specially emphasized were endotoxins of escherichia coli o24, o56, o104 ...19969072762
[structural and serologic characteristics of o-specific polysaccharides of hafnia alvei pcm 1185 and 1199].this work describes results of studies of o-specific oligosaccharide repeating units from lipopolysaccharides of two related hafnia alvei serotypes. the linkage between o-antigen and the core region in pcm 1199 lps has been also established. the o-acetyl residues present in the polysaccharides are involved in formation of epitopes.19969072768
structural studies of the o-specific polysaccharide of hafnia alvei strain 1209 lipopolysaccharide.the structure of the o-specific side chains of the hafnia alvei strain 1209 lipopolysaccharide has been investigated. methylation analysis and 1h-nmr and 13c-nmr spectroscopy were the principal methods used. it is concluded that the polysaccharide is composed of pentasaccharide repeating units that have the following structure: -->3)-beta-d-galp-(1-->4)-alpha-d-glcp-(1-->4)-beta-d-glepa-(1--> 3)-beta-d-galpnac-(1 --> 4 increases 1 alpha-l-rhap the relative intensity of the signals from the termi ...19968647107
the occurrence of glycine in bacterial lipopolysaccharides.the aminoacyl analysis of endotoxic lipopolysaccharides (lps) isolated from several bacteria revealed essential amounts of glycine, among the inherent lps components. significant amounts of the glycine was detected in lipopolysaccharides isolated from over 30 strains of escherichia, salmonella, hafnia, citrobacter and shigella species. glycine as a single amino acid was found only in a core part of lps. molar ratio of glycine in core oligosaccharide fraction ranged from 0.2 to 0.6 per 3 heptoses ...19968739188
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...19968742354
structure of the o-specific polysaccharide, containing a glycerol phosphate substituent, of hafnia alvei strain 1220 lipopolysaccharide.the o-specific polysaccharide of the lipopolysaccharide produced by hafnia alvei strain 1220 contained d-glucose, d-galactose, n-acetyl-d-glucosamine, n-acetyl-l-fucosamine (2-acetamido-2,6-dideoxy-l-galactose), glycerol, and phosphate. it was proved by composition and methylation analyses, smith degradation, dephosphorylation, and one- and two-dimensional 1h nmr spectroscopy to be a teichoic acid-like polymer with a branched hexasaccharide repeating unit of the following structure. [sequence: s ...19968765061
clinical significance of extraintestinal hafnia alvei isolates from 61 patients and review of the literature.hafnia alvei is a gram-negative bacterium that is rarely isolated from human specimens and is rarely considered to be pathogenic. it has been associated with gastroenteritis, meningitis, bacteremia, pneumonia, nosocomial wound infections, endophthalmitis, and a buttock abscess. we studied 80 h. alvei isolates recovered from 61 patients within a period of 30 months. h. alvei was cultured from sites that included the respiratory tract (n = 38), the gastrointestinal tract (n = 16), and the urogenit ...19968783707
the sensitivity of hafnia alvei strains to the bactericidal effect of serum.most hafnia alvei strains are sensitive to the bactericidal action of normal bovine serum (nbs) as well as to a serum in which the alternative pathway of complement activation has been thermally blocked. introduction of polysaccharides (ps) to nbs lowers the bactericidal effect. in a serum in which the alternative pathway of complement activation is blocked, ps completely cancels the bacterial effect.19968821399
bacteremia caused by hafnia alvei in a human immunodeficiency virus-infected child. 19968822302
prolyl aminopeptidase is also present in enterobacteriaceae: cloning and sequencing of the hafnia alvei enzyme-gene and characterization of the expressed enzyme.the hafnia alvei prolyl aminopeptidase gene (hpap) was cloned and sequenced, the expressed enzyme (hpap) was purified to homogeneity and thoroughly characterized. an open reading frame of 1,281 bp was found to code for the enzyme, resulting in a protein of 427 amino acids with a molecular weight of 48,577. hpap resembles the aeromonas sobria enzyme, having 45% identity and the same distinctive properties with respect to size and substrate specificities. both enzyme show similar chromatographic b ...19968830041
structural studies of the o-specific chain of hafnia alvei strain 32 lipopolysaccharide.the structure of the o-specific side chain of the hafnia alvei strain 32 lipopolysaccharide has been investigated. methylation analysis, partial acid hydrolysis, smith degradations, nmr spectroscopy, maldi-tof and fab mass spectrometry in combination with collision-induced decomposition ms/ms were the principal methods used. it is concluded that the polysaccharide is composed of pentasaccharide repeating units having the following structure which is partially o-acetylated in the 2- (20%) and 3- ...19968870241
characterization of the eaea gene from rabbit enteropathogenic escherichia coli strain rdec-1 and comparison to other eaea genes from bacteria that cause attaching-effacing lesions.a number of enteric pathogens, including enteropathogenic (epec) and enterohemorrhagic (ehec) escherichia coli, hafnia alvei, a strain of citrobacter freundii, and rabbit epec strain rdec-1 cause attaching-effacing (ae) lesions in the gut mucosa. these bacteria have a pathogenicity cassette (locus of enterocyte effacement or lee) containing the eaea gene. this gene encodes intimin, an outer membrane protein required for production of ae lesions. rdec-1, a non-invasive enteropathogen in young rab ...19968900070
specific spoilage organisms in breweries and laboratory media for their detection.the gram positive bacteria are generally regarded as the most hazardous beer spoilage organisms in modern breweries, especially the lactobacilli: l. brevis, l. lindneri, l. curvatus, l. casei, l. buchneri, l. coryneformis, l. plantarum, l. brevisimilis, l. malefermentans and l. parabuchneri and the pediococci: p damnosus, p. inopinatus and p. dextrinicus. micrococcus kristinae is the only species within the micrococci relevant to brewing. the gram negative strictly anaerobic bacteria are apparen ...19968913814
structural study and serological characterisation of the o-specific polysaccharide of hafnia alvei pcm 1185, another hafnia o-antigen that contains 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucose.the o-specific polysaccharide of h. alvei strain pcm 1185 contains d-glucose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, and 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]-d-glucose (qui3nacyl) in the ratios 2:1:1:1 as well as o-acetyl groups. on the basis of sugar and methylation analyses of the polysaccharide before and after chemical modifications (o-deacetylation, carboxyl reduction, smith degradation), as well as 1h and 13c nmr spectroscopy, including 1d sequential, selective spin-decouplin ...19968916544
characterization of espc, a 110-kilodalton protein secreted by enteropathogenic escherichia coli which is homologous to members of the immunoglobulin a protease-like family of secreted proteins.enteropathogenic escherichia coli (epec) secretes at least five proteins. two of these proteins, espa and espb (previously called eaeb), activate signal transduction pathways in host epithelial cells. while the role of the other three proteins (39, 40, and 110 kda) remains undetermined, secretion of all five proteins is under the control of pera, a known positive regulator of several epec virulence factors. on the basis of amino-terminal protein sequence data, we cloned and sequenced the gene wh ...19968932311
heterogeneity in phenotypic and genotypic characteristics among strains of hafnia alvei.hafnia alvei is an emerging human pathogen associated with sporadic cases and outbreaks of diarrhea. bangladeshi isolates of h. alvei possess the escherichia coli attaching and effacing (eaea) gene and demonstrate an attaching and effacing phenotype. in the present study we examined 11 canadian h. alvei isolates and strain 19,982 from bangladesh to determine if the formation of attaching and effacing lesions is a property shared among multiple isolates. attaching and effacing lesions were detect ...19968940433
structure of the o-specific polysaccharide of hafnia alvei pcm 1222 containing 2-aminoethyl phosphate.the o-specific polysaccharide of h. alvei strain pcm 1222 has a branched hexasaccharide repeating unit containing d-galactose, l-rhamnose, d-ribose, d-galacturonic acid, and 2-acetamido-2-deoxy-d-glucose in the ratios 1:2:1:1:1, as well as 2-aminoethyl phosphate (etnp) and o-acetyl groups in nonstoichiometric amounts. the polysaccharide was modified by carboxyl reduction, o-deacetylation, and dephosphorylation with 48% hydrofluoric acid, the last reaction being accompanied by removal of the late ...19969002188
re-speciation of the original reference strains of serovars in the citrobacter freundii (bethesda-ballerup group) antigenic scheme of west and edwards.the antigenic scheme for the bethesda-ballerup group of bacteria established by west and edwards in 1954 has continued to be applied as a serotyping scheme for citrobacter freundii. in 1993, however, the classification of the citrobacter was drastically revised and the species c. freundii redefined by brenner et al. accordingly, to judge the propriety to continuously use a single antigenic scheme for the c. freundii complex, the 90 reference strains listed in the antigenic scheme for c. freundii ...19969013489
optical properties of hafnia and coevaporated hafnia:magnesium fluoride thin films.mixed films of hfo(2) and mgf(2) were prepared by the e-beam coevaporation of two source materials with or without the presence of ion assistance. optical properties and hardness of mixed films were compared among various compositions that were achieved by controlling the deposition rate of mgf(2). results obtained from pure materials confirmed the densification of hfo(2) and the increased absorption in mgf(2) with a higher discharge voltage. a refractive index of a composite film from 1.38 to 1 ...199621102941
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...199516535048
[evaluation of the usefulness of tests for production of beta-d-glucuronidase and propylene glycol utilization for the differentiation of enterobacteriaceae rods].the aim of the study was to inquire about the diagnostic usefulness of determining the activity of glucuronidase and utilisation of propylene glycol in enterobacteriaceae rods. the study included 1511 strains: 411- e. coli, 278 - klebsiella, 231 - salmonella, 159 - yersinia, 97 - citrobacter, 75 - shigella and 260 strains representing 6 other kinds of enteric rods. determination was performed in a liquid medium containing in 1 ml 25 mcg mug and 100 mcg onpg. propylene glycol (pg) utilisation was ...19958833927
lipopolysaccharide core region of hafnia alvei: serological characterization.covalent glycoconjugates containing, as a ligand lipopolysaccharide core, oligosaccharides of hafnia alvei standard strain atcc 13337 and r mutant 1 m were used to produce anti-h. alvei core antibodies. the sera obtained were tested in rocket immunoelectrophoresis, immunoblotting and elisa using h. alvei lipopolysaccharides of various strains. the experiments were carried out to study the antigenic relationships between lipopolysaccharide core regions in the h. alvei genus.19957719279
structural elucidation of the biological repeating unit of o-specific polysaccharide from citrobacter serotype o41.sugar analysis of the o-specific polysaccharide produced by citrobacter serotype o41 revealed the presence of a hexasaccharide repeating unit, which includes the unusual 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactosyl residue (fuc3nacyl). the structure of the repeating unit was determined by extensive use of homo- and heteronuclear two-dimensional nmr spectroscopy, including the application of long-range 1h-13c correlation experiments and noe studies to establish the sequence of sugar u ...19957744044
acid-base chemical mechanism of aspartase from hafnia acid-base chemical mechanism is proposed for hafnia alvei aspartase in which a proton is abstracted from c-3 of the monoanionic form of l-aspartate by an enzyme general base with a pk of 6.3-6.6 in the absence and presence of mg2+. the resulting carbanion is presumably stabilized by delocalization of electrons into the beta-carboxyl with the assistance of a protonated enzyme group in the vicinity of the beta-carboxyl. ammonia is then expelled with the assistance of a general acid group that t ...19957793969
3-deoxy-octulosonic-acid-containing hexasaccharide fragment of unusual core type isolated from hafnia alvei 2 lipopolysaccharide.the hexasaccharide containing 3-deoxy-octulosonic acid (kdo) was isolated from the carbohydrate material obtained after the mild acid hydrolysis of lipopolysaccharide of hafnia alvei strain 2. the hexasaccharide was purified by gel filtration on bio-gel p-4 and p-2 columns. on the basis of sugar and methylation analyses, one- and two-dimensional nmr spectroscopic methods, the hexasaccharide was identified as [formula: see text] the tetrasaccharide linked to position 7 of kdo is also part of the ...19957867651
structure of the hafnia alvei strain pcm 1188 o-specific polysaccharide.the lipopolysaccharide was extracted from cells of hafnia alvei pcm 1188 strain and, after mild acid hydrolysis, the o-specific polysaccharide isolated and characterized. on the basis of sugar and methylation analysis, fab mass spectrometry and nmr spectroscopy of the polysaccharide and oligosaccharides obtained after smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the o-specific polysaccharide was shown to be the pentasaccharide: [formula: see text]19958556734
[gram-negative bacteria contaminating the process of producing lysine].the authors have isolated gram-negative bacteria of the enterobacteriaceae family from the culture liquid of industrial fermenters with low yield of lysine. most of them possessed the characters typical of klebsiella pneumoniae and escherichia coli, the rest were identified as the representatives of genera proteus, providencia, enterobacter, hafnia. strains of klebsiella pneumoniae, e.coli, proteus rettgeri manifested lysine-decarboxylase activity. the capacity of some strains to destruct lysine ...19958563944
structure of the o-specific polysaccharide of hafnia alvei 1204 containing 3,6-dideoxy-3-formamido-d-glucose.the o-specific polysaccharide of hafnia alvei strain 1204 has a hexasaccharide repeating unit containing d-mannose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, and 3,6-dideoxy-3-formamido-d-glucose (qui3nfo) in the ratios 2:1:1:1:1 as well as o-acetyl groups. on the basis of methylation analysis of the intact, carboxyl-reduced, and smith-degraded polysaccharide as well as 1d and 2d nmr spectroscopy, including 1d total correlation spectroscopy, 1d noe spectr ...19958565006
attributes of microbial associations of meat growing as xenic batch cultures in a meat juice at 4 degrees c.strains of gram-positive (carnobacterium piscicola, leuconostoc mesenteroides subsp. mesenteroides, brochothrix thermosphacta) and gram-negative (pseudomonas fragi and hafnia alvei) bacteria isolated from minced lamb packaged under a modified atmosphere were cultivated in a meat (lamb) juice at 4 degrees c. carbohydrates were catabolised in the order glucose > glucose 6-phosphate during the development of the population. under an atmosphere enriched with carbon dioxide the gram-negative portion ...19957488524
characterization of hafnia alvei by biochemical tests, random amplified polymorphic dna pcr, and partial sequencing of 16s rrna gene.hafnia alvei strains which possess the attachment-effacement gene (eaea) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other h. alvei strains. we characterized diarrheal h. alvei strains, which were positive in the pcr test for the eaea gene, using biochemical tests not routinely used for identification of members of the family enterobacteriaceae, and compared them with eaea-negative strains isolated from different clinical and nonclin ...19957494030
lipopolysaccharide core region of hafnia alvei: structure elucidation using chemical methods, gas chromatography-mass spectrometry, and nmr spectroscopy.sugar and methylation analysis with the use of gas chromatography-mass spectrometry and 1h nmr spectroscopy proved that the core oligosaccharides isolated from lipopolysaccharides of eight hafnia alvei strains have the identical hexasaccharide skeleton. however, 1h, 31p heterocorrelated spectra showed that the phosphorylation pattern is not the same. the branched heptose for the atcc 13337, 1187, 2, 1191, 1196, 1220, and 481l strains is phosphorylated as in the following formula, where p = -o-p( ...19957535188
structural studies of the o-specific chain and a core hexasaccharide of hafnia alvei strain 1192 lipopolysaccharide.the structure of the o-specific side-chain and a core hexasaccharide of the hafnia alvei strain 1192 lipopolysaccharide has been investigated. methylation analysis, nmr spectroscopy, maldi-tof spectrometry, and various specific chemical degradations were the principal methods used. it is concluded that the polysaccharide is composed of hexasaccharide repeating-units having the following structure which is partially o-acetylated in the 2-position of the --> 4)-alpha-d-glc pa-(1-->(70%) and on dif ...19957539717
buttock abscess due to hafnia alvei. 19957620037
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