| comparative resistance of nonsporogenic bacteria to low-temperature gamma irradiation. | a total of 36 microorganisms, comprising 19 species of 11 genera, were screened for radiation resistance with (60)co gamma rays at a radiation temperatore of -80 +/- 2 c in phosphate buffer (ph 7.0) under vacuum. micrococcus radiodurans was the most resistant organism. an initial population of 2.8 x 10(5) cells per dose of this species survived 2.4 but not 2.7 mrad. of the remaining 18 species with initial populations of about 10(6) cells per dose, streptococcus faecium survived 0.9 to 1.5 mrad, ... | 1973 | 4572982 |
| nucleotide sequence divergence in the -chain-structural genes of tryptophan synthetase from escherichia coli, salmonella typhimurium, and aerobacter aerogenes. | two different estimates were obtained for the extent of nucleotide sequence divergence in the structural genes of the tryptophan synthetase alpha-chains of escherichia coli, salmonella typhimurium, and aerobacter aerogenes. one estimate was based on comparisons of the amino acid sequences of the respective alpha chains. the other was derived from measurements of the thermal stability of rna-dna hybrids formed with phage dna carrying the alpha-chain structural gene of e. coli and labeled messenge ... | 1973 | 4577791 |
| evaluation of radiometric system for detecting bacteremia. | an automated radiometric system (bactec, johnston laboratories) for detection of bacteremia was evaluated in parallel with a standard blood culture system in use in our laboratory. of 1,445 blood cultures from 484 patients with possible bacteremia, 106 sets of cultures (excluding 39 presumed contaminated), representing 56 patients, were positive by both methods. the conventional system yielded 85 positive cultures from 48 patients, whereas the bactec system yielded 84 positive cultures from 43 p ... | 1973 | 4584580 |
| pressure transducer method for measuring gas production by microorganisms. | a simple method for measuring gas production by microorganisms by using a pressure transducer to sense pressure buildup was developed and tested with members of the coliform group. the test system consisted of a 5.0 lb/in(2) pressure transducer and a pressure equalizer valve attached to the metal cap of a test tube (20 by 150 mm); gas pressure was recorded on a strip-chart recorder. gas pressure response curves consisted of (i) a lag period with no marked increase in pressure, (ii) a rapid press ... | 1974 | 4589123 |
| purification and characteristics of aerocin 2 produced by enterobacter aerogenes. | | 1973 | 4589300 |
| effects of minerals on the production of valine by aerobacter aerogenes. | | 1973 | 4590568 |
| ultrastructure of the capsule of klebsiella pneumoniae and slime of enterobacter aerogenes revealed by freeze etching. | | 1973 | 4591533 |
| evaluating the membrane fecal coliform test by using escherichia coli as the indicator organism. | the fecal coliform membrane filter method (mfc) currently used in water pollution analysis was evaluated by using two strains of escherichia coli, a known fecal coliform, as the indicator organism. a large relative error in the results obtained with this method was found to be dependent upon the brand of membrane filter employed, the medium, and the temperature of incubation. mfc densities varied between 10 and 60% of the densities determined by means of total bacteria counts and total coliform ... | 1974 | 4596755 |
| subphrenic abscess: comparison between operative and antibiotic management. | subphrenic abscess is still a significant hazard which complicates surgical procedures as well as certain abdominal catastrophes. this is a report of 88 patients with subphrenic abscess at st. vincent's hospital and medical center of new york from 1954 through 1971. there were 46 males and 42 females, ranging from 2 to 88 years. operations on the stomach, duodenum and biliary tract were the major causes. the causative organisms in order of frequency were: e coli (41.6%), staphylococcus (41.6%), ... | 1974 | 4601985 |
| procaryote phylogeny. i. concerning the relatedness of aerobacter aerogenes to escherichia coli. | | 1974 | 4606938 |
| the lactose system in klebsiella aerogenes v9a. 4. a comparison of the lac operons of klebsiella and escherichia coli. | | 1974 | 4618214 |
| ribitol dehydrogenase from klebsiella aerogenes. purification and subunit structure. | ribitol dehydrogenase has been purified to homogeneity from several strains of klebsiella aerogenes. one strain yields 3-6g of pure enzyme from 1kg of cells. the enzyme is a tetramer of four subunits, mol.wt. 27000. preliminary studies of the activity of the enzyme are reported. peptide ;maps' together with the amino acid composition indicate that the subunits are identical. | 1974 | 4618776 |
| a mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from klebsiella aerogenes. | the first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. the overall sequencing strategy evolved is asse ... | 1974 | 4618777 |
| changes in antibiotic sensitivity patterns of gram-negative bacilli in burns. | sensitivity tests with 12 antibiotics on 1,018 strains of gram-negative bacilli isolated in a burns unit between 1969 and 1971 showed some important differences from results in similar tests on a series of strains isolated between 1965 and 1967. these changes included the emergence of a large proportion of kanamycin-resistant strains of klebsiella aerogenes, proteus mirabilis, and escherichia coli and of smaller proportions of trimethoprim- and gentamicin-resistant strains; also the complete rep ... | 1972 | 4622793 |
| l-ribulose 5-phosphate 4-epimerase from aerobacter aerogenes. evidence for a role of divalent metal ions in the epimerization reaction. | | 1972 | 4623844 |
| micro-complement fixation in klebsiella classification. | the alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera aerobacter, klebsiella and enterobacter were compared by the micro-complement fixation technique. on the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to aerobacter aerogenes and enterobacter aerogenes be assigned to the genus klebsiella. | 1972 | 4624110 |
| cellobiose metabolism in aerobacter aerogenes. 3. cleavage of cellobiose monophosphate by a phospho- -glucosidase. | | 1972 | 4624114 |
| isolation and action pattern of maltohexaose producing amylase from aerobacter aerogenes. | | 1972 | 4636744 |
| studies of the acetate kinase-phosphotransacetylase and the butanediol-forming systems in aerobacter aerogenes. | mutants of aerobacter aerogenes devoid of acetate kinase and phosphotransacetylase activities were isolated by selection for resistance to fluoroacetate on lactate medium. the mutants were used to study the role of the acetate kinase-phosphotransacetylase system in growth on acetate and glucose. acetate kinase-negative and phosphotransacetylase-negative mutants were unable to grow on acetate minimal medium. their growth rates on glucose minimal medium were identical with that of the parent strai ... | 1972 | 4640502 |
| metabolism of gentiobiose in aerobacter aerogenes. | cleavage of gentiobiose in cell extracts of gentiobiose-grown aerobacter aerogenes was dependent on the presence of adenosine 5'-triphosphate (atp). the enzymes that participate in the overall reaction were shown to be a beta-glucoside kinase, which catalyzes the phosphorylation of gentiobiose with atp to form gentiobiose monophosphate [6-o-phosphoryl-beta-d-glucopyranosyl-(1 --> 6)-d-glucose], and a phospho-beta-glucosidase, which catalyzes the hydrolytic cleavage of gentiobiose monophosphate t ... | 1972 | 4640505 |
| evidence for vectorial phosphorylation of d-fructose by intact cells of aerobacter aerogenes. | extracellular d-fructose was metabolized preferentially over intracellular d-fructose in growing cells of a d-fructokinaseless mutant of aerobacter aerogenes. this finding is interpreted to indicate that phosphorylation of d-fructose by the membrane-bound phosphoenolpyruvate: d-fructose 1-phosphotransferase system in intact cells is coupled to unidirectional transport. | 1972 | 4640508 |
| [kinetics of the transformation of 1,2-propanediol to propionic aldehyde, catalyzed by glycerol dehydratase from aerobacter aerogenes]. | | 1972 | 4643736 |
| stimulation of growth on a deficient medium of a klebsiella aerogenes species by sulphonamide, para-amino benzoic acid, nitrofurantoin, and some other substances. | a strain of klebsiella aerogenes is described which was unable to grow from light inocula on 5% lysed horse blood diagnostic sensitivity test (dst) agar. it was able to grow on dst agar without added horse cells and macconkey and nutrient agar. growth on a deficient medium was promoted by sulphonamides and nitrofurantoin as well as by para-amino benzoic acid, para-amino salicylic acid, procaine hydrochloride, thymine, and uracil. | 1972 | 4648542 |
| the lactose system in klebsiella aerogenes v9a. 1. characteristics of two lac mutant phenotypes which revert to wild-type. | | 1972 | 4654235 |
| butirosin, a new aminoglycosidic antibiotic complex: antibacterial activity in vitro and in mice. | butirosin is a new aminoglycosidic antibiotic complex which has broad gram-negative and gram-positive inhibitory antibacterial activity, as well as some bactericidal properties. significantly susceptible bacteria include strains of staphylococcus aureus and streptococcus pyogenes, and pathogenic gram-negative species such as enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis and p. vulgaris, salmonella enteritidis and s. typhimurium, shigella flexneri and s. sonne ... | 1972 | 4670492 |
| evidence that the inducible phosphoenolpyruvate:d-fructose 1-phosphotransferase system of aerobacter aerogenes does not require "hpr". | | 1973 | 4712203 |
| the production of enzymes involved in exopolysaccharide synthesis in klebsiella aerogenes types 1 and 8. | | 1973 | 4717925 |
| regulation of the l-arabinose catabolic pathway in aerobacter aerogenes. | | 1973 | 4718778 |
| the lactose system in klebsiella aerogenes v9a. 2. galactoside permeases which accumulate lactose or melibiose. | | 1973 | 4731640 |
| regulation of d-xylose and d-arabitol catabolism by aerobacter aerogenes. | aerobacter aerogenes strain prl-r3 possesses inducible enzyme pathways for the catabolism of d-xylose and d-arabitol. d-xylose is the apparent inducer for d-xylose isomerase and d-xylulokinase. d-arabitol is the apparent inducer for d-arabitol dehydrogenase and a separate d-xylulokinase. both kinases had similar k(m) values and substrate specificities, and could not be separated by sucrose gradient centrifugation or polyacrylamide gel electrophoresis. they could be differentiated, however, by th ... | 1973 | 4734863 |
| s-acetyl phosphopantetheine: deacetyl citrate lyase s-acetyl transferase from klebsiella aerogenes. | | 1973 | 4741546 |
| derepression of arylsulfatase synthesis in aerobacter aerogenes by tyramine. | studies were made on the effect of tyramine on arylsulfatase synthesis in mutants of aerobacter aerogenes atcc 9621 deficient in enzymes involved in tyramine degradation. as shown previously, some sulfur compounds, such as inorganic sulfate, repressed enzyme synthesis while others, such as methionine, did not. tyramine caused derepression of enzyme synthesis, which is repressed by inorganic sulfate. the present work showed that, although tyramine readily derepressed arylsulfatase synthesis, meta ... | 1973 | 4745414 |
| further studies on anthranilate n-acetyltransferase and the metabolism of n-acetylanthranilic acid in aerobacter aerogenes. | | 1973 | 4748369 |
| phenotypic variability of the envelope proteins of klebsiella aerogenes. | | 1973 | 4762920 |
| [formation of glycerol dehydratase by a culture of aerobacter aerogenes, its partial purification and various properties]. | | 1973 | 4772149 |
| the occurrence of a stepwise-decreasing respiration rate during oxidative assimilation of different substrates by resting klebsiella aerogenes in a system open to oxygen. | the addition of an oxidizable substrate to a continuous culture of klebsiella aerogenes is known to cause an increased respiration rate that decreases in discrete steps as the added substrate is being exhausted. we have used a simple new technique to show that this phenomenon is also produced by washed, resting cells harvested from batch or continuous growth culture. the stepwise-decreasing respiration rate is caused by the exhaustion of different pools of intermediates. each plateau of respirat ... | 1973 | 4786531 |
| genetic transfer of nitrogen fixation from rhizobium trifolii to klebsiella aerogenes. | | 1974 | 4828191 |
| distribution of the isopropylmalate pathway to leucine among diverse bacteria. | alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ... | 1974 | 4829932 |
| effect of complex nutrients, vitamins and amino acids on valine production by aerobacter aerogenes. | | 1974 | 4836928 |
| synthesis of s-alkyl-l-cysteine from pyruvate, ammonia and alkyl-mercaptan by cysteine desulfhydrase of aerobacter aerogenes. | | 1974 | 4854588 |
| studies on the interaction of homologues of spermine with deoxyribonucleic acid and with bacterial protoplasts. | four homologues of the naturally occurring polyamine spermine, of the type h(2)n.[ch(2)](3).nh.[ch(2)] (n).nh.[ch(2)](3).nh(2) where n=2, 3, 5 and 6, have been synthesized. their ability to stabilize escherichia coli protoplasts against osmotic lysis was compared with that of spermine. all homologues were approximately as effective as spermine. the effect of low concentrations of the homologues on the t(m) of calf thymus dna and of aerobacter aerogenes dna in 0.03m-sodium chloride-1mm-potassium ... | 1967 | 4860546 |
| the reversal of phenylarsenoxide inhibition of keto acid oxidation in mitochondrial and bacterial suspensions by lipoic acid and other disulphides. | 1. inhibition of pyruvate oxidation in suspensions of aerobacter aerogenes cells and of isolated mitochondria from rat heart and liver by phenylarsenoxide is prevented by an excess of lipoic acid, whereas inhibition due to certain bivalent cations is not. 2. in both systems inhibition persists when the bacteria and mitochondria are recovered and resuspended in fresh media in the absence of the inhibitor. persistent inhibition due to preincubation with phenylarsenoxide, but not with the metal ion ... | 1967 | 4860547 |
| ribosyl and deoxyribosyl transfer by bacterial enzyme systems. | the enzymatic transfer of ribose and deoxyribose residues in pyrimidine nucleosides to purines was catalyzed by cell-free extracts of various bacteria. almost all the strains belonging to enterobacteriaceae were capable of catalyzing the transfer reactions. the transfer activities were also detected among some bacterial strains of other families: pseudomonadaceae, corynebacteriaceae, micrococcaceae, bacteriaceae, and bacillaceae. the rates of the transfer reactions were greatly enhanced in the p ... | 1967 | 4863982 |
| the cell size and macromolecular composition of aerobacter aerogenes in various systems of continuous culture. | | 1967 | 4864933 |
| the action of drugs on aerobacter aerogenes in various systems of continuous culture. | | 1967 | 4864934 |
| induction of adenosine deaminase in escherichia coli. | supplementing the salts-glucose medium of escherichia coli with adenine initiates induction of adenosine deaminase (adenosine aminohydrolase, ec 3.5.4.4), growth inhibition, and an increased potential for the net deamination of adenine. the extent and duration of these events are proportional to the initial adenine concentration and are dependent upon adenylate pyrophosphorylase and repression of histidine biosynthesis for maximal expression. the conversion of adenine to hypoxanthine, though lim ... | 1968 | 4874315 |
| the influence of bacteriocins on resistance to infection by gram-negative bacteria. ii. colicin action, transfer of colicinogeny, and transfer of antibiotic resistance in urinary infections. | dual urinary infections were produced in rats with colicinogenic escherichia coli cf1, elaborating colicin v in the urine, and colicine-sensitive e. coli 9224 by injecting each organism into the medulla of opposite kidneys. the colicin-sensitive organism was eradicated from the urine of 24.3% of rats and the degree of infection by e. coli 9224 reduced to less than half of the control group. colicin-resistant mutants of e. coli 9224 were not inhibited in mixed infections with colicin producing e. ... | 1968 | 4875004 |
| biosynthesis of diacetyl in bacteria and yeast. | both diacetyl and acetoin were produced by cell-free extracts and cultures of pseudomonas fluorescens, aerobacter aerogenes, lactobacillus brevis, and saccharomyces cerevisiae 299, whereas only acetoin was produced by cell-free extracts and cultures of streptococcus lactis, serratia marcescens, escherichia coli, and s. cerevisiae strains 513 and 522. cell-free extracts that produced diacetyl did not produce it from acetoin; they produced it from pyruvate, but only if acetyl-coenzyme a was was ad ... | 1968 | 4876127 |
| nonspecific toxicites in the mouse assay test for botulinum toxin. | in inoculated pack experiments on clostridium botulinum type e, unirradiated and 0.1-mrad irradiated haddock fillets often gave nonspecific toxicities by the mouse assay test for botulinum toxin. samples given 0.2-mrad radiation failed to produce nonspecific reactions. nonspecific deaths sometimes occurred within 24 hr after injection, although deaths between 24 and 48 hr were more common. the symptoms and the pattern of these deaths suggested a septicemia. heart-blood cultured from mice showing ... | 1968 | 4877496 |
| pathogenesis of coliform mastitis in cattle. i. classification of the etiologic agent (aerobacter aerogenes) and "natural" immune response to its antigens in cattle. | | 1968 | 4878528 |
| immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of enterobacteriaceae. | the reactive surface structures of alpha subunits of tryptophan synthase from escherichia coli, shigella dysenteriae, salmonella typhimurium, aerobacter aerogenes, and serratia marcescens were compared by measuring (i) their reactivities in micro-complement-fixation assays with antibodies directed specifically to e. coli wild-type alpha subunit, (ii) their reactivities in enzyme neutralization assays with the same antibodies, and (iii) their binding affinities for tryptophan synthase beta(2) sub ... | 1969 | 4887511 |
| comparison in normal and leukopenic cows of experimental mastitis due to aerobacter aerogenes or escherichia coli endotoxin. | | 1969 | 4888853 |
| oxidation d-malic and beta-alkylmalic acids wild-type and mutant strains of salmonella typhimurium and by aerobacter aerogenes. | a mutant strain of salmonella typhimurium (sl 1634 dml-51) capable of growth on d-malate as sole carbon source was shown to produce d-malic enzyme. this enzyme was absent in the parent wild-type strain which was unable to grow on d-malate. growth of the mutant on d-malate also resulted in a greatly increased level of beta-isopropylmalic enzyme compared with its level in the wild-type strain grown on citrate or l-malate. the d-malic and beta-isopropylmalic enzymes, both of which catalyze a nicoti ... | 1969 | 4889267 |
| polynucleotide sequence relationships among members of enterobacteriaceae. | polynucleotide relationships were examined among many representatives of the enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. the amount of deoxyribonucleic acid (dna) that reassociated was dependent, especially in interspecific reactions, on the annealing temperature. in only three cases: escherichia coli-shigella flexneri, salmonella typhimurium-s. typhi, and proteus mirabilis-p. vulgaris, was relative interspecific duplex formation 80% or higher. in most ca ... | 1969 | 4891264 |
| taxonomic investigations on expressed and cryptic phospho-beta-glucosidases in enterobacteriaceae. | in the enterobacteriaceae, beta-glucosides are catabolized by a complex system formed of three permeases, with partly overlapping substrate specificities, and two hydrolytic enzymes, phospho-beta-glucosidase a and b, which hydrolyze only phosphorylated beta-glucosides. some enterobacteriaceae such as klebsiella-aerobacter (enterobacter) possess the complete system; others possess only parts of it or may have a cryptic phospho-beta-glucosidase activity without permease activity. a screening test ... | 1969 | 4897109 |
| structural analysis of colanic acid from escherichia coli by using methylation and base-catalysed fragmentation. comparison with polysaccharides from other bacterial sources. | essentially the same methanolysis products were obtained after methylation of the slime and capsular polysaccharides from escherichia coli k12 (s53 and s53c sub-strains) and the slime polysaccharides from e. coli k12 (s61), aerobacter cloacae n.c.t.c. 5290 and salmonella typhimurium sl1543. these were the methyl glycosides of 2-o-methyl-l-fucose, 2,3-di-o-methyl-l-fucose, 2,3-di-o-methyl-d-glucuronic acid methyl ester, 2,4,6-tri-o-methyl-d-glucose, 2,4,6-tri-o-methyl-d-galactose and the pyruvic ... | 1969 | 4902692 |
| biologic properties of lethal factors produced by enterobacter aerogenes. | | 1969 | 4905500 |
| evaluation of iron-reducing bacteria in soil and the physiological mechanism of iron-reduction in aerobacter aerogenes. | | 1968 | 4916832 |
| inhibitory effects of ethidium bromide on the growth kinetics of klebsiella aerogenes, adaptation to ethidium and cross-resistance to proflavine. | | 1969 | 4917184 |
| the isolation of o-acetylated fragments from the k antigen of escherichia coli 08:k27 (a):h by the action of phage-induced enzymes from klebsiella aerogenes. | | 1970 | 4918138 |
| biosynthesis of n-acetylanthranilic acid by aromatic auxotrophs of aerobacter aerogenes and escherichia coli. | | 1970 | 4923918 |
| genetic determination of bacteriocin production by enterobacter aerogenes. | | 1970 | 4924098 |
| factors affecting phosphate uptake by aerobacter aerogenes in a system relating cell numbers to 32p uptake. | the uptake of phosphate, from media limited in this ion, by resting cells of aerobacter aerogenes has been investigated and shown to be dependent upon several factors. an incubation medium composed of 10(-2)m k(+), 5 x 10(-3)m mg(2+), 1 mg of glucose per ml, and 1 muci of (32)po(4) (3-) per ml, buffered at ph 6.55 with 0.05 mn-2-hydroxyethyl-piperazine-n'-2-ethanesulfonic acid (hepes), was found to stimulate optimum accumulation of (32)p-orthophosphate. the temperature of incubation, incubation ... | 1971 | 4928606 |
| n-acetylanthranilic acid biosynthesis in aerobacter aerogenes and escherichia coli. | | 1971 | 4931932 |
| transfer of aerocinogeny in enterobacter aerogenes. | | 1971 | 4938010 |
| biotin production and utilization in a sewage treatment lagoon. | biotin, in a sewage oxidation lagoon also receiving potato processing wastes, was observed to increase two logs during the summer period of waste stabilization and then to decline to near earlier concentrations. three organisms, aerobacter aerogenes, chlorella vulgaris, and thiocapsa floridana, were at least partially responsible for these fluctuations; the latter two organisms were associated with biotin utilization and the former with biotin production. since copious quantities of biotin are p ... | 1971 | 4939123 |
| some relationships between r-factor and chromosomal -lactamase in gram-negative bacteria. | 1. the beta-lactamases specified by klebsiella aerogenes 418 and the r-factor r-7268 have been partially purified. 2. the molecular weights of the k. aerogenes strains 418 and 373, aerobacter cloacae 53, r-7268 and r-tem beta-lactamases were all about 20000; that of the enzymes from escherichia coli strains 419 and 214t was about 31000. 3. these enzymes were also compared by means of their k(m) values for benzylpenicillin and ampicillin, and their behaviour on starch-gel electrophoresis. 4. the ... | 1971 | 4942448 |
| enzyme alterations in tyrosine and phenylalanine auxotrophs of salmonella typhimurium. | the enzyme activities specified by the tyra and phea genes were studied in wildtype strain salmonella typhimurium and in phenylalanine and tyrosine auxotrophs. as in aerobacter aerogenes and escherichia coli, the wild-type enzymes of salmonella catalyze two consecutive reactions: chorismate --> prephenate --> 4-hydroxy-phenylpyruvate (tyra), and chorismate --> prephenate --> phenylpyruvate (phea). a group of tyra mutants capable of interallelic complementation had altered enzymes which retained ... | 1971 | 4945189 |
| [transfer of colicinogenic factors from escherichia coli to aerobacter aerogenes]. | | 1971 | 4947726 |
| metabolic injury to bacteria. i. effect of freezing and storage on the requirements of aerobacter aerogenes and escherichia coli for growth. | | 1966 | 4958829 |
| bacteriostatic action of progesterone on staphylococci and other microorganisms. | yotis, william (loyola university, hines, ill.), and ronald stanke. bacteriostatic action of progesterone on staphylococci and other microorganisms. j. bacteriol. 92:1285-1289. 1966.-progesterone has been examined in vitro for antibacterial activity against 10 microorganisms. turbidimetric and manometric techniques were used to assay the antibacterial activity of progesterone. the organisms tested consisted of staphylococcus aureus, s. epidermidis, gaffkya tetragena, bacillus subtilis, listeria ... | 1966 | 4958877 |
| detection and identification of bacteria by gas chromatography. | ether extracts of cultures of 29 strains representing 6 species of bacillus, and of individual strains of escherichia coli, aerobacter aerogenes, and pseudomonas aeruginosa were examined in a gas chromatograph by use of flame ionization and electron capture detectors. among the products detected were compounds with the chromatographic characteristics of acetic, propionic, and butyric acids, ethyl alcohol, diacetyl, acetoin, and 2,3-butanediol. the differences in peak areas of the various product ... | 1966 | 4959077 |
| damaging effects of ethylenediaminetetra-acetate and penicillins on permeability barriers in gram-negative bacteria. | 1. the permeability barrier against benzylpenicillin has been found to be passive in four strains of penicillinase-producing gram-negative bacteria (three of klebsiella aerogenes and one of escherichia coli). 2. if the three k. aerogenes strains are grown in the presence of sub-inhibitory concentrations of benzylpenicillin, ampicillin or phenethicillin the resultant bacterial cells have deficient permeability barriers. concentrations of ampicillin or benzylpenicillin less than one-tenth of those ... | 1966 | 4961352 |
| influence of egg albumin-induced leukopenia on experimental aerobacter aerogenes mastitis and on natural infection of mammary gland with coagulase-negative staphylococcus in a cow. | | 1967 | 4964095 |
| oxalacetate decarboxylase of aerobacter aerogenes. i. inhibition by avidin and requirement for sodium ion. | | 1967 | 4965256 |
| the biosynthesis of tyrosine in aerobacter aerogenes: partial purification of the t protein. | | 1967 | 4965258 |
| rapid and sensitive detection of bacteria by gas chromatography. | a gas chromatograph fitted with electron capture and flame ionization detectors was employed for the rapid detection of bacteria by analysis for their metabolic products. the presence of proteus vulgaris, streptococcus faecalis, s. liquefaciens, escherichia coli b, bacillus cereus, and b. popilliae was detected in 2 to 4 hr in media inoculated with less than 10(4) cells per ml, whereas a 7- to 12-hr growth period was required for the detection of products formed in cultures of serratia marcescen ... | 1968 | 4967452 |
| effects in hyperoxia upon microorganisms. i. membrane culture technique for exposing cells directly to test atmospheres. | a membrane culture technique was developed for directly exposing microorganisms to test atmospheres. inhibition and killing were calculated from comparisons with air-grown cultures. direct colony counts were used with low inocula. with mass inocula, plate colony counts and optical-density measurements were made on resuspended filter populations. bacteria, including escherichia coli, were more sensitive to oxygen than previously reported. with inocula of a few hundred cells per membrane, five of ... | 1968 | 4967757 |
| influence of growth condition on the concentration of potassium in bacillus subtilis var. niger and its possible relationship to cellular ribonucleic acid, teichoic acid and teichuronic acid. | 1. mg(2+)-limited bacillus subtilis var. niger, growing in a chemostat in a simple salts medium, contained considerably more potassium and phosphorus than mg(2+)-limited aerobacter aerogenes growing in a similar medium at corresponding dilution rates. 2. growth of the bacillus in a k(+)-limited environment did not lower the cellular potassium and phosphorus contents, the molar proportions of cell-bound magnesium, potassium, rna (as nucleotide) and phosphorus being approximately constant at 1:13: ... | 1968 | 4976492 |
| capacity of aspartic acid to increase the bacterial count on suspensions of escherichia coli after freezing. | the addition of 2% trypticase to a minimal salts-glucose plating medium increased the bacterial count of frozen and thawed suspensions of escherichia coli 451b cells, even when precautions were taken to remove toxic trace elements from the plating diluent. hydrolysis of the trypticase with hcl or h(2)so(4) reduced its count-increasing activity. treatment of the h(2)so(4) hydrolysate with a cation-exchange resin greatly improved its capacity to replace trypticase. addition of a mixture of amino a ... | 1969 | 4977483 |
| degradation of uric acid by certain aerobic bacteria. | we have isolated and identified nine cultures of aerobic bacteria capable of growing on an elective medium containing uric acid as the only source of carbon, nitrogen, and energy. four of these cultures were identified as aerobacter aerogenes, two as klebsiella pneumoniae, and the remainder as serratia killiensis, pseudomonas aeruginosa, and bacillus species. another culture identified as p. fluorescens required both glucose and uric acid for growth. when 23 laboratory stock cultures were inocul ... | 1968 | 4979099 |
| organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria. | ultracentrifugation in sucrose density gradients was employed to estimate the molecular weights and to determine possible physical aggregation of the five enzymes catalyzing steps two to six in the prechorismic acid portion of the polyaromatic synthetic pathway in six species of bacteria: escherichia coli, salmonella typhimurium, aerobacter aerogenes, bacillus subtilis, pseudomonas aeruginosa, and streptomyces coelicolor. the five enzymes were not aggregated in extracts of any of the species exa ... | 1969 | 4979440 |
| enzymatic analysis of the requirement for sodium in aerobic growth of salmonella typhimurium on citrate. | na(+) was required for the aerobic growth of salmonella typhimurium on citrate, but not on l-malate, glucose, or glycerol. the maximal growth rate and the maximal total growth occurred with 6 to 7 mm na(+). na(+) could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). sonically treated extracts of citrate-grown cells contained the enzymes of the citrate fermentation pathway (citritase and oxalacetate decarboxylase) and all of the enzymes of the citric acid cycle. thus, two separate rou ... | 1969 | 4980062 |
| the oxidative degradation of benzoate and catechol by klebsiella aerogenes (aerobacter aerogenes). | | 1970 | 4987140 |
| experimental technique for studying aerosols of lyophilized bacteria. | an experimental technique is presented for studying aerosols generated from lyophilized bacteria by using escherichia coli b, bacillus subtilis var. niger, enterobacter aerogenes, and pasteurella tularensis. an aerosol generator capable of creating fine particle aerosols of small quantities (10 mg) of lyophilized powder under controlled conditions of exposure to the atmosphere is described. the physical properties of the aerosols are investigated as to the distribution of number of aerosol parti ... | 1970 | 4992657 |
| the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. the effect of 2-oxobutyrate upon the enzyme activity. | | 1971 | 4997852 |
| interaction of nalidixic acid with klebsiella (aerobacter) aerogenes growing in continuous culture. | | 1970 | 5002637 |
| a potassium-dependent citric acid transport system in aerobacter aerogenes. | | 1972 | 5015237 |
| transport of citric acid by aerobacter aerogenes. | | 1972 | 5017252 |
| l-ribulose 5-phosphate 4-epimerase from aerobacter aerogenes. kinetic isotope effect with tritiated substrate. | | 1972 | 5027743 |
| cellobiose metabolism in aerobacter aerogenes. ii. phosphorylation of cellobiose with adenosine 5'-triphosphate by a -glucoside kinase. | | 1972 | 5030625 |
| effect of several clay minerals and humic acid on the survival of klebsiella aerogenes exposed to ultraviolet irradiation. | the effect of various clay minerals and humic acid on the survival of klebsiella aerogenes exposed to ultraviolet (uv) irradiation was investigated. a protective effect was observed and found to depend on the specific light absorption and light scattering properties of the clay minerals and the humic acid used. the higher the specific absorption, the better was the survival of k. aerogenes after uv irradiation. bacterial survival was lower in clays saturated with divalent cations (ca, zn) than i ... | 1972 | 5031559 |
| molecular characterization of pullulanase from aerobacter aerogenes. | | 1972 | 5043329 |
| myo-inositol transport in aerobacter aerogenes. | | 1972 | 5049009 |
| a study of the effect of growth conditions on chemostat-grown klebsiella aerogenes and kinetic changes of a 500-nm absorption band. | | 1972 | 5049019 |
| an improved purification of cell-bound pullulanase from aerobacter aerogenes. | | 1972 | 5065077 |
| venereal transmission of klebsiella aerogenes in a thoroughbred stud from a persistently infected stallion. | | 1972 | 5067026 |
| beta-galactosidase synthesis in klebsiella aerogenes growing in continuous culture. | | 1972 | 5071389 |
| pullulanase synthesis in klebsiella (aerobacter) aerogenes. | | 1972 | 5075238 |
| the effect of growth conditions on the chemical composition and endotoxicity of walls of aerobacter aerogenes n.c.t.c. 418. | | 1972 | 5076218 |