| performances of vitek 2 colorimetric cards for identification of gram-positive and gram-negative bacteria. | the purpose of this study was to evaluate the new vitek 2 identification cards that use colorimetric reading to identify gram-positive and gram-negative bacteria (gp and gn cards, respectively) in comparison to fluorimetric cards (id-gpc and id-gnb, respectively). a total of 580 clinical isolates and stock collection strains belonging to 116 taxa were included in the study. of the 249 gram-positive strains tested with both the id-gpc and gp cards, 218 (87.5%) and 235 (94.4%) strains were correct ... | 2005 | 16145083 |
| clinical characteristics and prognosis of acute bacterial meningitis in elderly patients over 65: a hospital-based study. | to examine the clinical characteristics of bacterial meningitis in elderly patients. | 2011 | 22204457 |
| the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identifying clinically relevant bacteria: a comparison with automated microbiology system. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has been developed as a new-type soft ionization mass spectrometry in the recent year. increasing number of clinical microbiological laboratories consider it as an innovate approach for bacterial identification. | 2014 | 24822117 |
| elevated plasma matrix metalloproteinase-9 and its correlations with severity of disease in patients with ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) increases patient mortality and medical expenditure, and a real-time and reliable method for the rapid diagnosis of vap may help reduce fatal complications. matrix metalloproteinases-9 (mmp-9) is considered significant in the pathogenesis of lung inflammation and infection. therefore, we examined its relationship with the clinical course of vap. this retrospective observational study recruited 30 healthy volunteers, 12 patients who used mechanical ventilatio ... | 2016 | 27499696 |
| evaluation of the filmarray blood culture identification panel: results of a multicenter controlled trial. | sepsis is a major cause of morbidity, mortality, and increased medical expense. rapid diagnosis improves outcomes and reduces costs. the filmarray blood culture identification panel (biofire diagnostics llc, salt lake city, ut), a highly multiplexed pcr assay, can identify 24 etiologic agents of sepsis (8 gram-positive, 11 gram-negative, and 5 yeast species) and three antimicrobial resistance genes (meca, vana/b, and blakpc) from positive blood culture bottles. it provides results in about 1 h w ... | 2016 | 26739158 |
| identification and characterization of repetitive extragenic palindromes (rep)-associated tyrosine transposases: implications for rep evolution and dynamics in bacterial genomes. | bacterial repetitive extragenic palindromes (reps) compose a distinct group of genomic repeats. they usually occur in high abundance (>100 copies/genome) and are often arranged in composite repetitive structures - bacterial interspersed mosaic elements (bimes). in bimes, regularly spaced reps are present in alternating orientations. bimes and reps have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. th ... | 2010 | 20085626 |
| evaluation of the wider system, a new computer-assisted image-processing device for bacterial identification and susceptibility testing. | the wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. it has been adapted to be able to read and interpret commercial microscan panels. two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family enterobacteriaceae, 25 nonfermentative gram-negative rods [nfgnrs], and 81 gram-positive cocci) were tested. in addition, 100 enterobacterial strains with known beta-lactam re ... | 2000 | 10747104 |
| emerging organisms in a tertiary healthcare set up. | one-tenth of all infectious diseases are attributable to emerging organisms. as emerging organisms sporadically affect a relatively small percentage of population they are not studied at large. this study was aimed at studying the characteristics of emerging organisms encountered from various clinical samples in an apex tertiary care multispeciality teaching and research hospital. | 2013 | 24843199 |
| emerging organisms in a tertiary healthcare set up. | one-tenth of all infectious diseases are attributable to emerging organisms. as emerging organisms sporadically affect a relatively small percentage of population they are not studied at large. this study was aimed at studying the characteristics of emerging organisms encountered from various clinical samples in an apex tertiary care multispeciality teaching and research hospital. | 2013 | 24843199 |
| comparative evaluation of automated reading versus visual interpretation with the bbl crystal enteric/nonfermenter identification system in a clinical setting. | | 2007 | 17520304 |
| self-inflicted chronic bacterial keratoconjunctivitis using self semen. | this case report describes a case of self-inflicted chronic bacterial keratoconjunctivitis involving the patient's own semen. a 20-year-old male soldier was referred to our clinic for the evaluation of refractory chronic bacterial conjunctivitis. over the previous 4 months, he had been treated for copious mucous discharge, conjunctival injection, and superficial punctate keratitis in both eyes at an army hospital and a local eye clinic. despite the use of topical and systemic antibiotics accordi ... | 2013 | 24311933 |
| pediatric multicenter evaluation of the verigene gram-negative blood culture test for rapid detection of inpatient bacteremia involving gram-negative organisms, extended-spectrum beta-lactamases, and carbapenemases. | we evaluated the investigational use only (iuo) version of the rapid verigene gram-negative blood culture test (bc-gn), a microarray that detects 9 genus/species targets (acinetobacter spp., citrobacter spp., enterobacter spp., escherichia coli/shigella spp., klebsiella oxytoca, klebsiella pneumoniae, proteus spp., pseudomonas aeruginosa, and serratia marcescens) and 6 antimicrobial resistance determinants (blactx-m, blakpc, blandm, blavim, blaimp, and blaoxa) directly from positive blood cultur ... | 2014 | 24759724 |
| comparative study of maldi-tof ms and vitek 2 in bacteria identification. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. this study aimed to evaluate the accuracy of maldi-tof ms in clinical microbiology diagnosis by comparing it with commonly-used vitek 2 or gene sequencing. | 2014 | 24822115 |
| real-time pcr taqman assay for rapid screening of bloodstream infection. | sepsis is one of the main causes of mortality and morbidity. the rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. this study aimed to accelerate the detection and discrimination of gram-positive (gp) and gram-negative (gn) bacteria and candida species in blood culture samples by molecular methods. | 2014 | 24393579 |
| the complications of sinusitis in a tertiary care hospital: types, patient characteristics, and outcomes. | objective. to study the complications of sinusitis in a referral hospital and the outcome of the treatment according to the type of complication. methods. a retrospective study was performed on patients with sinusitis who were admitted to a referral hospital from 2003 to 2012. the data for the sinusitis patients who had complications were reviewed. results and discussion. eighty-five patients were included in the study, of whom 50 were male (58.8%). fourteen of the cases were less than 15 years ... | 2015 | 25722725 |
| comparison of vitek gni and gni+ cards for identification of gram-negative bacteria. | the gni+ card has been developed by biomerieux vitek as an improvement over the gni card for the identification of certain species of aerobic and facultative anaerobic bacteria. in this study, we tested 304 organisms from 30 different species on both the gni and gni+ cards. the gni card correctly identified 285 (93.8%) of the isolates tested, and the gni+ card correctly identified 287 (94.4%) of the isolates tested. the average time to reporting was 4.1 h for the gni+ card compared to 5.7 h for ... | 1998 | 9705437 |
| rapid identification of bacteria from positive blood cultures by fluorescence-based pcr-single-strand conformation polymorphism analysis of the 16s rrna gene. | bacteremia continues to result in significant morbidity and mortality, particularly in patients who are immunocompromised. currently, patients with suspected bacteremia are empirically administered broad-spectrum antibiotics, as definitive diagnosis relies upon the use of blood cultures, which impose significant delays in and limitations to pathogen identification. to address the limitations of growth-based identification, the sequence variability of the 16s rrna gene of bacteria was targeted fo ... | 2000 | 10655337 |
| characterization of a novel chaperone/usher fimbrial operon present on kpgi-5, a methionine trna gene-associated genomic island in klebsiella pneumoniae. | several strain-specific klebsiella pneumoniae virulence determinants have been described, though these have almost exclusively been linked with hypervirulent liver abscess-associated strains. through pcr interrogation of integration hotspots, chromosome walking, island-tagging and fosmid-based marker rescue we captured and sequenced kpgi-5, a novel genomic island integrated into the met56 trna gene of k. pneumoniae kr116, a bloodstream isolate from a patient with pneumonia and neutropenic sepsis ... | 2012 | 22520965 |
| characterization of pseudomonas aeruginosa lpxt reveals dual positional lipid a kinase activity and co-ordinated control of outer membrane modification. | gram-negative bacteria have evolved modification machinery to promote a dynamic outer membrane in response to a continually fluctuating environment. the kinase lpxt, for example, adds a phosphate group to the lipid a moiety of some gram-negatives including escherichia coli and salmonella enterica. lpxt activity is inhibited under conditions that compromise membrane integrity, resulting instead in the addition of positively charged groups to lipid a that increase membrane stability and provide re ... | 2014 | 25223756 |
| development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry. | bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. aiming at the development of a rapid, simplified and efficient protocol, we developed and compared two in-house preparatory methods for the direct identification of bacteria from positive blood culture flasks (bd bactec fx system) by using matrix-assisted ... | 2015 | 26546000 |
| the identification and characterization of non-coding and coding rnas and their modified nucleosides by mass spectrometry. | the analysis of ribonucleic acids (rna) by mass spectrometry has been a valuable analytical approach for more than 25 years. in fact, mass spectrometry has become a method of choice for the analysis of modified nucleosides from rna isolated out of biological samples. this review summarizes recent progress that has been made in both nucleoside and oligonucleotide mass spectral analysis. applications of mass spectrometry in the identification, characterization and quantification of modified nucleo ... | 2014 | 25616408 |
| the identification and characterization of non-coding and coding rnas and their modified nucleosides by mass spectrometry. | the analysis of ribonucleic acids (rna) by mass spectrometry has been a valuable analytical approach for more than 25 years. in fact, mass spectrometry has become a method of choice for the analysis of modified nucleosides from rna isolated out of biological samples. this review summarizes recent progress that has been made in both nucleoside and oligonucleotide mass spectral analysis. applications of mass spectrometry in the identification, characterization and quantification of modified nucleo ... | 2014 | 25616408 |
| global patterns of protein domain gain and loss in superkingdoms. | domains are modules within proteins that can fold and function independently and are evolutionarily conserved. here we compared the usage and distribution of protein domain families in the free-living proteomes of archaea, bacteria and eukarya and reconstructed species phylogenies while tracing the history of domain emergence and loss in proteomes. we show that both gains and losses of domains occurred frequently during proteome evolution. the rate of domain discovery increased approximately lin ... | 2014 | 24499935 |
| a bioinformatics analysis reveals a group of mocr bacterial transcriptional regulators linked to a family of genes coding for membrane proteins. | the mocr bacterial transcriptional regulators are characterized by an n-terminal domain, 60 residues long on average, possessing the winged-helix-turn-helix (whth) architecture responsible for dna recognition and binding, linked to a large c-terminal domain (350 residues on average) that is homologous to fold type-i pyridoxal 5'-phosphate (plp) dependent enzymes like aspartate aminotransferase (aat). these regulators are involved in the expression of genes taking part in several metabolic pathwa ... | 2016 | 27446613 |
| comparative activity of cefepime with several antimicrobials against aerobic gram-negative and gram-positive organisms isolated from patients across canada in 1993. | to compare the activity of cefepime, a fourth-generation cephalosporin, with several available antimicrobials, in vitro susceptibility studies were carried out on bacteria commonly associated with various infections, including sepsis. ten tertiary care hospital laboratories in six provinces provided 1276 clinically relevant isolates of aerobic gram-negative bacilli and gram-positive cocci during 1993. when the activity of each of the antimicrobials was determined against all isolates submitted, ... | 1995 | 22514408 |
| simplified technique for detection of significant bacteriuria by microscopic examination of urine. | a comparative study of microscopic examination of 10 microl (simplified loop technique) and 50 microl (traditional drop technique) of uncentrifuged gram-stained urine specimens for detection of significant bacteriuria was carried out. the results demonstrated that the 10-microl loop technique can be used as an alternative to the 50-microl drop technique for presumptive diagnosis of urinary-tract infection in bacteriological practice, with the advantages of greater rapidity and ease of performanc ... | 1998 | 9508322 |
| in vitro activities of sch27899 alone and in combination with 17 other antimicrobial agents. | sch27899, an everninomicin antibiotic, was tested for its in vitro activity against 718 bacterial isolates representing 27 species. the enterobacteriaceae and nonenteric gram-negative bacilli were resistant to > or = 8.0 microg/ml, but all others were inhibited by < or = 1.0 microg/ml. when tested in combination with 17 other antimicrobial agents against 110 strains, sch27899 demonstrated no significant antagonism or synergy. consequently, combination therapy is not contraindicated. | 1999 | 10582895 |
| comparison of agar dilution, disk diffusion, microscan, and vitek antimicrobial susceptibility testing methods to broth microdilution for detection of fluoroquinolone-resistant isolates of the family enterobacteriaceae. | fluoroquinolone resistance appears to be increasing in many species of bacteria, particularly in those causing nosocomial infections. however, the accuracy of some antimicrobial susceptibility testing methods for detecting fluoroquinolone resistance remains uncertain. therefore, we compared the accuracy of the results of agar dilution, disk diffusion, microscan walk away neg combo 15 conventional panels, and vitek gns-f7 cards to the accuracy of the results of the broth microdilution reference m ... | 1999 | 9986809 |
| monotherapy with intravenous followed by oral high-dose ciprofloxacin versus combination therapy with ceftazidime plus amikacin as initial empiric therapy for granulocytopenic patients with fever. | the aim of the present study was to obtain clinical experience with the use of high-dose ciprofloxacin as monotherapy for the treatment of febrile neutropenia episodes (granulocyte count, <500/mm(3)) compared to a standard regimen and to clarify whether ciprofloxacin administration may be switched to the oral route. in a prospective randomized study ciprofloxacin was given at 400 mg three times a day (t.i.d.) for at least 72 h followed by oral administration at 750 mg twice a day (b.i.d). that r ... | 2000 | 11083625 |
| how novel methods can help discover more information about foodborne pathogens. | considerable emphasis is being placed on quantitative risk assessment modelling as a basis for regulation of trade in food products. however, for models to be accurate, information about the behaviour of potential pathogens in foods needs to be available. the question is how to obtain this knowledge in a simple and cost effective way. one technique that has great potential is the use of reporter bacteria which have been genetically modified to express a phenotype that can be easily monitored, su ... | 2000 | 18159282 |
| in vitro activities of sitafloxacin (du-6859a) and six other fluoroquinolones against 8,796 clinical bacterial isolates. | the in vitro activities of sitafloxacin, ciprofloxacin, trovafloxacin, levofloxacin, clinafloxacin, gatifloxacin, and moxifloxacin against 5,046 gram-negative bacteria, 3,344 gram-positive cocci, and 406 anaerobes were determined. sitafloxacin was the most active agent against gram-positive cocci and anaerobes. against enterobacteriaceae and nonfermenters, its activity was either equivalent to or better than that of clinafloxacin. | 2000 | 10722524 |
| survival of aerobic and anaerobic bacteria in purulent clinical specimens maintained in the copan venturi transystem and becton dickinson port-a-cul transport systems. | recovery of aerobic and anaerobic bacteria from clinical specimens maintained in the copan venturi transystem and the becton dickinson port-a-cul transport was assessed. of 54 anaerobes, 53 were recovered after 4 h, and 52 were recovered after 24 h, from both systems. after 48 h, 45 and 50 were recovered from the two systems, respectively. | 2000 | 10655410 |
| potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance. | there has recently been much controversy surrounding the increased use of antibacterial substances in a wide range of consumer products and the possibility that, as with antibiotics, indiscriminate use of biocides might contribute to the overall pattern of susceptibility in the general environment and in the clinic. such speculation, based on the isolation of resistant mutants from in vitro monoculture experiments, is not reflected by an emergence of biocide-resistant strains in vivo. this revie ... | 2003 | 12692093 |
| clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants. | twenty percent of very-low-birth-weight (<1500 g) preterm infants experience a serious systemic infection, and despite advances in neonatal intensive care and antimicrobials, mortality is as much as threefold higher for these infants who develop sepsis than their counterparts without sepsis during their hospitalization. outcomes may be improved by preventative strategies, earlier and accurate diagnosis, and adjunct therapies to combat infection and protect the vulnerable preterm infant during an ... | 2004 | 15258097 |
| effect of medium age and supplementation with the biocatalytic oxygen-reducing reagent oxyrase on in vitro activities of tigecycline against recent clinical isolates. | in determining the quality control limits for the clinical laboratory standards institute-recommended quality control organisms with tigecycline, a number of inconsistencies in the results were encountered that appeared to be related to the age of the mueller-hinton broth ii. this study was performed to examine the effect of medium age and supplementation with oxyrase on the activity of tigecycline using a large number of clinical isolates. | 2005 | 16127070 |
| controlled clinical comparison of plastic versus glass bottles of bact/alert pf medium for culturing blood from children. | the plastic pediatric bact/alert (biomérieux, durham, n.c.) pf (ppf) is a new nonvented aerobic culture medium in a clear plastic bottle designed to prevent breakage. we compared the performance of the new ppf bottle to that of the present glass bact/alert pf bottle for the recovery of microorganisms as well as for the time to detection of growth in samples of blood obtained for culture from children. we found that the ppf and pf bottles were comparable for recovery of microorganisms and that th ... | 2005 | 15635011 |
| in vitro antibacterial activity of ce-156811, a novel analog derived from hygromycin a. | we evaluated a novel truncated hygromycin a analog in which the furanose ring was replaced with a 2-fluoro-2-cyclopropylethyl substituent for its activity against multidrug resistant gram-positive bacteria and compared its activity to the activities of linezolid, quinupristin-dalfopristin, and vancomycin. ce-156811 demonstrated robust in vitro activity against gram-positive bacteria that was comparable to that of linezolid. | 2008 | 18426902 |
| solubilization of insoluble inorganic phosphate by burkholderia cepacia da23 isolated from cultivated soil. | a mineral phosphate solubilizing bacterium, burkholderia cepacia da23 has been isolated from cultivated soils. phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. when 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. mineral phosphate solubilization was directly related to the ph drop by the strain. analysis of the culture medium by high pressure liquid c ... | 2008 | 24031195 |
| pharmacokinetic-pharmacodynamic-model-guided doripenem dosing in critically ill patients. | the growing number of infections caused by multidrug-resistant pathogens has prompted a more rational use of available antibiotics given the paucity of new, effective agents. monte carlo simulations were utilized to determine the appropriateness of several doripenem dosing regimens based on the probability of attaining the critical drug exposure metric of time that drug concentrations remain above the drug mic (t>mic) for 35% (and lower thresholds) of the dosing interval in >80 to 90% of the pop ... | 2010 | 20385857 |
| comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. | bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is an emerging technology newly applied to the problem of bacterial species identification. we evaluated two maldi-tof ms systems with 720 consecutively isolated bacterial colonies under routine clinical laboratory conditions. isolates were analyzed ... | 2010 | 20164271 |
| rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish) from colony and blood culture material. | multi-drug-resistant strains of the acinetobacter baumannii complex cause nosocomial infections. rapid identification of acinetobacter spp. is desirable in order to facilitate therapeutic or hygiene decisions. we evaluated a newly designed dna probe that can be used under standard conditions in both a microwave oven and a slide chamber for the rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish). using fish, the new probe correctly identified 81/81 acinetobacte ... | 2011 | 24516735 |
| opportunistic microorganisms in patients undergoing antibiotic therapy for pulmonary tuberculosis. | antimicrobial therapy may cause changes in the resident oral microbiota, with the increase of opportunistic pathogens. the aim of this study was to compare the prevalence of candida, staphylococcus, pseudomonas and enterobacteriaceae in the oral cavity of fifty patients undergoing antibiotic therapy for pulmonary tuberculosis and systemically healthy controls. oral rinsing and subgingival samples were obtained, plated in sabouraud dextrose agar with chloramphenicol, mannitol agar and macconkey a ... | 2011 | 24031759 |
| multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. | the use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. we aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. selective cultures for enterobacteria (ent), non-fermenting gram-negative rods (nfr) and gram-positive cocci (gpc) were obtained from water samples collected in two different year seasons. the isolated bacteria were biochemically identified and a ... | 2012 | 22972388 |
| globally dispersed mobile drug-resistance genes in gram-negative bacterial isolates from patients with bloodstream infections in a us urban general hospital. | mobile drug-resistance genes with identical nucleic acid sequences carried by multidrug-resistant escherichia coli strains that cause community-acquired infections are becomingly increasingly dispersed worldwide. over a 2-year period, we analysed gram-negative bacterial (gnb) pathogens from the blood of inpatients at an urban public hospital to determine what proportion of these isolates carried such globally dispersed drug-resistance genes. of 376 gnb isolates, 167 (44 %) were escherichia coli, ... | 2012 | 22493279 |
| enhanced diagnostic yields of bacteremia and candidemia in blood specimens by pcr-electrospray ionization mass spectrometry. | a prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. a total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were recruited during a 10-month study. pcr amplification coupled with electrospray ionization mass spectromet ... | 2013 | 23966503 |
| pcr-reverse blot hybridization assay for screening and identification of pathogens in sepsis. | rapid and accurate identification of the pathogens involved in bloodstream infections is crucial for the prompt initiation of appropriate therapy, as this can decrease morbidity and mortality rates. a pcr-reverse blot hybridization assay for sepsis, the reverse blot hybridization assay (reba) sepsis-id test, was developed; it uses pan-probes to distinguish gram-positive and -negative bacteria and fungi. in addition, the assay was designed to identify bacteria and fungi using six genus-specific a ... | 2013 | 23447637 |
| multilaboratory study of the biomic automated well-reading instrument versus microscan walkaway for reading microscan antimicrobial susceptibility and identification panels. | this study compared the biomic automated well reader results to the microscan walkaway results for reading microscan antimicrobial susceptibility and identification panels at four different sites. routine fresh clinical isolates and quality control (qc) organisms were tested at each study site. a total of 46,176 microscan panel drug-organism combinations were read. the biomic category agreement for 3,117 gram-negative bacteria was 98.4%, with 1.4% minor and 0.2% major discrepancies. the biomic c ... | 2013 | 23486713 |
| exploring preterm birth as a polymicrobial disease: an overview of the uterine microbiome. | infection is a leading cause of preterm birth (ptb). a focus of many studies over the past decade has been to characterize microorganisms present in the uterine cavity and document any association with negative pregnancy outcome. a range of techniques have been used to achieve this, including microbiological culture and targeted polymerase chain reaction assays, and more recently, microbiome-level analyses involving either conserved, phylogenetically informative genes such as the bacterial 16s r ... | 2014 | 25505898 |
| susceptibility to antibiotics in urinary tract infections in a secondary care setting from 2005-2006 and 2010-2011, in são paulo, brazil: data from 11,943 urine cultures. | urinary tract infection (uti) has a high incidence and recurrence, therefore, treatment is empirical in the majority of cases. | 2014 | 25076433 |
| performance of mass spectrometric identification of bacteria and yeasts routinely isolated in a clinical microbiology laboratory using maldi-tof ms. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is an emerging technology newly applied to identifying bacterial and yeast strains. the aim of this study was to evaluate the clinical performance of the vitek(®) ms system in the identification of bacteria and yeast strains routinely isolated from clinical samples. | 2014 | 24822114 |
| prevalence of porphyromonas gingivalis four rag locus genotypes in patients of orthodontic gingivitis and periodontitis. | porphyromonas gingivalis is considered as a major etiological agent in periodontal diseases and implied to result in gingival inflammation under orthodontic appliance. rag locus is a pathogenicity island found in porphyromonas gingivalis. four rag locus variants are different in pathogenicity of porphyromonas gingivalis. moreover, there are different racial and geographic differences in distribution of rag locus genotypes. in this study, we assessed the prevalence of porphyromonas gingivalis and ... | 2013 | 23593379 |
| three decades of beta-lactamase inhibitors. | since the introduction of penicillin, beta-lactam antibiotics have been the antimicrobial agents of choice. unfortunately, the efficacy of these life-saving antibiotics is significantly threatened by bacterial beta-lactamases. beta-lactamases are now responsible for resistance to penicillins, extended-spectrum cephalosporins, monobactams, and carbapenems. in order to overcome beta-lactamase-mediated resistance, beta-lactamase inhibitors (clavulanate, sulbactam, and tazobactam) were introduced in ... | 2010 | 20065329 |
| reverse transcription-pcr-electrospray ionization mass spectrometry for rapid detection of biothreat and common respiratory pathogens. | electrospray ionization mass spectrometry (esi-ms) analysis of reverse transcription (rt)-pcr amplicons from human respiratory samples allows for broad pathogen identification approximately 8 h after collection. we investigated the performance characteristics of a high-throughput rt-pcr-coupled esi-ms assay for distinguishing biothreat (bt) agents from common bacterial, fungal, and viral respiratory pathogens in bronchoalveolar lavage (bal) fluid specimens from subjects with suspected respirator ... | 2013 | 23903543 |
| maldi-tof mass spectrometry: a powerful tool for clinical microbiology at hôpital principal de dakar, senegal (west africa). | our team in europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms). to evaluate the utility of maldi-tof ms in tropical africa in collaboration with local teams, we installed an apparatus in the hôpital principal de dakar (senegal), performed routine identification of isolates, and confirmed or completed their identification in france. in the case of discordance or a la ... | 2015 | 26716681 |
| multicenter evaluation of the bruker maldi biotyper ca system for the identification of clinical aerobic gram-negative bacterial isolates. | the prompt and accurate identification of bacterial pathogens is fundamental to patient health and outcome. recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) have revolutionized bacterial identification in the clinical laboratory, but uniform incorporation of this technology in the u.s. market has been delayed by a lack of fda-cleared systems. in this study, we conducted a multicenter evaluation of the maldi biotyper ca (mbt-ca) system ... | 2015 | 26529504 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| review on the antimicrobial resistance of pathogens from tracheal and endotracheal aspirates of patients with clinical manifestations of pneumonia in bacolod city in 2013. | microbiological content specifically bacterial and fungal etiologies from tracheal aspirates in a tertiary hospital in bacolod city was reviewed for baseline information. a total of 130 tracheal aspirates were subjected for culture to isolate and identify the pathogen and determine their susceptibilities to various antibiotics. productions of certain enzymes responsible for antibiotic resistance like esbl (extended spectrum beta-lactamase), metallo-β-lactamase, and carbapenemase were also studie ... | 2015 | 26904750 |
| the alpha-defensin test for periprosthetic joint infection responds to a wide spectrum of organisms. | the alpha-defensin test has been previously demonstrated to be highly accurate in the diagnosis of prosthetic joint infection (pji), nearly matching the musculoskeletal infection society definition for pji. however, the relationship between alpha-defensin levels and differing infecting organism has not yet been investigated. | 2015 | 25631170 |
| ceftazidime-avibactam versus doripenem for the treatment of complicated urinary tract infections, including acute pyelonephritis: recapture, a phase 3 randomized trial program. | the global emergence of carbapenem-resistant enterobacteriaceae highlights the urgent need to reduce carbapenem dependence. the phase 3 recapture program compared the efficacy and safety of ceftazidime-avibactam and doripenem in patients with complicated urinary tract infection (cuti), including acute pyelonephritis. | 2016 | 27313268 |
| origin and evolution of antibiotic resistance: the common mechanisms of emergence and spread in water bodies. | the environment, and especially freshwater, constitutes a reactor where the evolution and the rise of new resistances occur. in water bodies such as waste water effluents, lakes, and rivers or streams, bacteria from different sources, e.g., urban, industrial, and agricultural waste, probably selected by intensive antibiotic usage, are collected and mixed with environmental species. this may cause two effects on the development of antibiotic resistances: first, the contamination of water by antib ... | 2012 | 22303296 |
| microarray-based identification of bacteria in clinical samples by solid-phase pcr amplification of 23s ribosomal dna sequences. | the rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. we describe a dna microarray-based pcr approach for the quick detection and identification of bacteria from cervical swab specimens from mares. this on-chip pcr method combines the amplification of a variable region of bacterial 23s ribosomal dna and the simultaneous sequence-specific detection on a solid phase. the solid phase contains bacterial species-specific primers co ... | 2004 | 15004052 |
| zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes. | a total of 232 isolates of gram-negative bacteria were recovered from mammals, reptiles, and birds housed at asa zoological park, hiroshima prefecture, japan. forty-nine isolates (21.1%) showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. pcr and dna sequencing identified class 1 and class 2 integrons and many beta-lactamase-encoding genes, in addition to a novel ampc beta-lactamase gene, bla(cmy-26). furthermore, the plasmid-mediated quinolone resista ... | 2007 | 17720829 |
| signals, regulatory networks, and materials that build and break bacterial biofilms. | biofilms are communities of microorganisms that live attached to surfaces. biofilm formation has received much attention in the last decade, as it has become clear that virtually all types of bacteria can form biofilms and that this may be the preferred mode of bacterial existence in nature. our current understanding of biofilm formation is based on numerous studies of myriad bacterial species. here, we review a portion of this large body of work including the environmental signals and signaling ... | 2009 | 19487730 |
| cumulative clinical experience from over a decade of use of levofloxacin in urinary tract infections: critical appraisal and role in therapy. | the treatment of urinary tract infections (utis) continues to evolve as common uropathogens increasingly become resistant to previously active antimicrobial agents. in addition, bacterial isolates, which were once considered to be either colonizers or contaminants, have emerged as true pathogens, likely related to the more complex array of settings where health care is now delivered. even though the reliability of many antimicrobial agents has become less predictable, the fluoroquinolone group o ... | 2011 | 22114510 |
| microbial manipulation of the amyloid fold. | microbial biofilms are encased in a protein, dna, and polysaccharide matrix that protects the community, promotes interactions with the environment, and helps cells adhere together. the protein component of these matrices is often a remarkably stable, β-sheet-rich polymer called amyloid. amyloids form ordered, self-templating fibers that are highly aggregative, making them a valuable biofilm component. some eukaryotic proteins inappropriately adopt the amyloid fold, and these misfolded protein a ... | 2012 | 23108148 |
| multiple transcription-activating sequences regulate the rsmz regulatory small rna of pseudomonas brassicacearum. | the muts-rpos region is known to be a highly polymorphic segment of the chromosome owing to horizontal gene transfer and evolutionary processes. in pseudomonas, muts-fdxa-rsmz-rpos organization is highly conserved, as well as the promoter region of the rsmz small rna (srna)-encoding gene. one exception to this conservation is in pseudomonas brassicacearum, where a 308-nucleotide (nt) sequence, predicted to form a hairpin structure in single-stranded dna (ssdna), is inserted between the rpos and ... | 2012 | 22773788 |
| promiscuous cross-seeding between bacterial amyloids promotes interspecies biofilms. | amyloids are highly aggregated proteinaceous fibers historically associated with neurodegenerative conditions including alzheimers, parkinsons, and prion-based encephalopathies. polymerization of amyloidogenic proteins into ordered fibers can be accelerated by preformed amyloid aggregates derived from the same protein in a process called seeding. seeding of disease-associated amyloids and prions is highly specific and cross-seeding is usually limited or prevented. here we describe the first stud ... | 2012 | 22891247 |
| regulation and evolution of malonate and propionate catabolism in proteobacteria. | bacteria catabolize malonate via two pathways, encoded by the mdc and mat genes. in various bacteria, transcription of these genes is controlled by the gntr family transcription factors (tfs) matr/mdcy and/or the lysr family transcription factor mdcr. propionate is metabolized via the methylcitrate pathway, comprising enzymes encoded by the prp and acn genes. prpr, the fis family sigma 54-dependent transcription factor, is known to be a transcriptional activator of the prp genes. here, we report ... | 2012 | 22505679 |
| phylogenomic analysis identifies gene gains that define salmonella enterica subspecies i. | comparative methods for analyzing whole genome sequence (wgs) data enable us to assess the genetic information available for reconstructing the evolutionary history of pathogens. we used the comparative approach to determine diagnostic genes for salmonella enterica subspecies i. s. enterica subsp. i strains are known to infect warm-blooded organisms regularly while its close relatives tend to infect only cold-blooded organisms. we found 71 genes gained by the common ancestor of salmonella enteri ... | 2013 | 24204679 |
| molecular basis of active copper resistance mechanisms in gram-negative bacteria. | copper is a metallic element that is crucial for cell metabolism; however, in extended concentrations, it is toxic for all living organisms. the dual nature of copper has forced organisms, including bacteria, to keep a tight hold on cellular copper content. this challenge has led to the evolution of complex mechanisms that on one hand enable them to deliver the essential element and on the other to protect cells against its toxicity. such mechanisms have been found in both eukaryotic and prokary ... | 2013 | 24072389 |
| [bacterial ecology of nosocomial infection in intensive care unit of laquintinie hospital douala, cameroon]. | | 2013 | 23785545 |
| impact of tongue biofilm removal on mechanically ventilated patients. | to evaluate the effectiveness of a tongue cleaner in the removal of tongue biofilm in mechanically ventilated patients. | 2013 | 23887759 |
| the disulfide bonding system suppresses csgd-independent cellulose production in escherichia coli. | the bacterial extracellular matrix encases cells and protects them from host-related and environmental insults. the escherichia coli master biofilm regulator csgd is required for the production of the matrix components curli and cellulose. csgd activates the diguanylate cyclase adra, which in turn stimulates cellulose production through cyclic di-gmp (c-di-gmp). here, we identified and characterized a csgd- and adra-independent cellulose production pathway that was maximally active when cultures ... | 2014 | 25112475 |
| the first 1000 cultured species of the human gastrointestinal microbiota. | the microorganisms that inhabit the human gastrointestinal tract comprise a complex ecosystem with functions that significantly contribute to our systemic metabolism and have an impact on health and disease. in line with its importance, the human gastrointestinal microbiota has been extensively studied. despite the fact that a significant part of the intestinal microorganisms has not yet been cultured, presently over 1000 different microbial species that can reside in the human gastrointestinal ... | 2014 | 24861948 |
| identification of glutathione (gsh)-independent glyoxalase iii from schizosaccharomyces pombe. | reactive carbonyl species (rcs), such as methylglyoxal (mg) and glyoxal (go), are synthesized as toxic metabolites in living systems. mechanisms of rcs detoxification include the glutathione (gsh)-dependent system consisting of glyoxalase i (glo1) and glyoxalase ii (glo2), and gsh-independent system involving glyoxalase iii (glo3). hsp31 and dj-1 proteins are weakly homologous to each other and belong to two different subfamilies of the dj-1/hsp31/pfpi superfamily. recently, the escherichia coli ... | 2014 | 24758716 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| the molecular characterisation of escherichia coli k1 isolated from neonatal nasogastric feeding tubes. | the most common cause of gram-negative bacterial neonatal meningitis is e. coli k1. it has a mortality rate of 10-15 %, and neurological sequelae in 30-50 % of cases. infections can be attributable to nosocomial sources, however the pre-colonisation of enteral feeding tubes has not been considered as a specific risk factor. | 2015 | 26497222 |
| nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers. | treatment of micro-polluted source water is receiving increasing attention because of environmental awareness on a global level. we isolated and identified aerobic denitrifying bacteria zoogloea sp. n299, acinetobacter sp. g107, and acinetobacter sp. 81y and used these to remediate samples of their native source water. we first domesticated the isolated strains in the source water, and the 48-h nitrate removal rates of strains n299, g107, and 81y reached 33.69%, 28.28%, and 22.86%, respectively, ... | 2015 | 25867475 |
| direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry. | early diagnosis of urinary tract infections (utis) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. microbiological confirmation takes 24 to 48 h. the use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. in addition, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a widely established technique in clinical microbiology laborator ... | 2016 | 26818668 |
| gfp-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of white mustard. | the introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. in this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pprobe-nt) bearing ... | 2011 | 22661921 |
| gfp-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of white mustard. | the introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. in this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pprobe-nt) bearing ... | 2011 | 22661921 |
| characteristics of urinary tract infection pathogens and their in vitro susceptibility to antimicrobial agents in china: data from a multicenter study. | this study assessed the characteristics of pathogens identified in clinical isolates from patients with urinary tract infection (uti) and their in vitro sensitivity to commonly used antibiotics in the clinical setting in china. | 2013 | 24334199 |
| so depression is an inflammatory disease, but where does the inflammation come from? | we now know that depression is associated with a chronic, low-grade inflammatory response and activation of cell-mediated immunity, as well as activation of the compensatory anti-inflammatory reflex system. it is similarly accompanied by increased oxidative and nitrosative stress (o&ns), which contribute to neuroprogression in the disorder. the obvious question this poses is 'what is the source of this chronic low-grade inflammation?' | 2013 | 24228900 |
| the flavin reductase msue is a novel nitroreductase that can efficiently activate two promising next-generation prodrugs for gene-directed enzyme prodrug therapy. | bacterial nitroreductase enzymes that can efficiently catalyse the oxygen-independent reduction of prodrugs originally developed to target tumour hypoxia offer great potential for expanding the therapeutic range of these molecules to aerobic tumour regions, via the emerging cancer strategy of gene-directed enzyme prodrug therapy (gdept). two promising hypoxia prodrugs for gdept are the dinitrobenzamide mustard pr-104a, and the nitrochloromethylbenzindoline prodrug nitro-cbi-dei. we describe here ... | 2013 | 24202330 |
| occurrence of enterobacteriaceae in raw meat and in human samples from egyptian retail sellers. | the present study was performed to assess the presence of enterobacteriaceae in raw meat and handlers in egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). a total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at mansoura city, egypt. fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial is ... | 2014 | 27379312 |
| wastewater treatment using integrated anaerobic baffled reactor and bio-rack wetland planted with phragmites sp. and typha sp. | the purpose of this study is to examine the potential use of anaerobic baffled reactor (abr) followed by bio-rack wetland planted with phragmites sp. and typha sp. for treating domestic wastewater generated by small communities (751 mg cod/l, 500 scod mg/l, 348 mg bod5/l). two parallel laboratory-scale models showed that the process planted with phragmites sp. and typha sp. are capable of removing cod by 87% & 86%, scod by 90% & 88%, bod5 by 93% & 92%, tss by 88% & 86%, tn by 79% & 77%, po4-p by ... | 2014 | 25379186 |
| molecular method for detection of total coliforms in drinking water samples. | this work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different pcr assays targeting the lacz, wecg, and 16s rrna genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). pcr assays were first compared to the culture-based colilert and mi agar methods to determine their ability to detect 147 coliform strains representing 76 species of enterobacteriaceae encountered in fecal and ... | 2014 | 24771030 |
| maldi-tof identification of the human gut microbiome in people with and without diarrhea in senegal. | in africa, there are several problems with the specific identification of bacteria. recently, maldi-tof mass spectrometry has become a powerful tool for the routine microbial identification in many clinical laboratories. | 2014 | 24784934 |
| characterization of an allylic/benzyl alcohol dehydrogenase from yokenella sp. strain wzy002, an organism potentially useful for the synthesis of α,β-unsaturated alcohols from allylic aldehydes and ketones. | a novel whole-cell biocatalyst with high allylic alcohol-oxidizing activities was screened and identified as yokenella sp. wzy002, which chemoselectively reduced the c=o bond of allylic aldehydes/ketones to the corresponding α,β-unsaturated alcohols at 30°c and ph 8.0. the strain also had the capacity of stereoselectively reducing aromatic ketones to (s)-enantioselective alcohols. the enzyme responsible for the predominant allylic/benzyl alcohol dehydrogenase activity was purified to homogeneity ... | 2014 | 24509923 |
| markers of oxidative stress and neuroprogression in depression disorder. | major depression is multifactorial disorder with high prevalence and alarming prognostic in the nearest 15 years. several mechanisms of depression are known. neurotransmitters imbalance and imbalance between neuroprogressive and neuroprotective factors are observed in major depression. depression is accompanied by inflammatory responses of the organism and consequent elevation of proinflammatory cytokines and increased lipid peroxidation are described in literature. neuropsychiatric disorders in ... | 2015 | 26078821 |
| procalcitonin levels in gram-positive, gram-negative, and fungal bloodstream infections. | procalcitonin (pct) can discriminate bacterial from viral systemic infections and true bacteremia from contaminated blood cultures. the aim of this study was to evaluate pct diagnostic accuracy in discriminating gram-positive, gram-negative, and fungal bloodstream infections. a total of 1,949 samples from patients with suspected bloodstream infections were included in the study. median pct value in gram-negative (13.8 ng/ml, interquartile range (iqr) 3.4-44.1) bacteremias was significantly highe ... | 2015 | 25852221 |
| unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway. | 3-dehydroquinate dehydratase (dhqase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. in this study, 3180 prokaryotic genomes were examined and 459 dhqase sequences were retrieved. based on sequence analysis and their original hosts, 38 dhqase genes were selected for chemical synthesis. the selected dhqases were translated into new dna sequences according to the genetic codon usage bias by both escherichia coli and corynebacterium glutamicum. ... | 2015 | 25852984 |
| thigh abscess and necrotizing fasciitis following an inside-out transobturator tape intervention: a case report. | tension-free vaginal transobturator tapes are used worldwide in the treatment of urinary incontinence in women. very few severe complications have been described following this procedure, with no standard treatment yet established. | 2016 | 27256334 |
| evaluation of heavy metal removal from wastewater in a modified packed bed biofilm reactor. | for the effective application of a modified packed bed biofilm reactor (pbbr) in wastewater industrial practice, it is essential to distinguish the tolerance of the system for heavy metals removal. the industrial contamination of wastewater from various sources (e.g. zn, cu, cd and ni) was studied to assess the impacts on a pbbr. this biological system was examined by evaluating the tolerance of different strengths of composite heavy metals at the optimum hydraulic retention time (hrt) of 2 hour ... | 2016 | 27186636 |
| a multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. | functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. moreover, highly diverse environments are used as metagenomic sources. however, such methods suffer from low hit rates of positive clones and hence the dis ... | 2016 | 26822785 |
| aerobic bacterial microflora of broad-snouted caiman (caiman latirostris) oral cavity and cloaca, originating from parque zoológico arruda câmara, paraíba, brazil. | the objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's samples, collected from broad-snouted caiman (caiman latirostris), born and bred in captivity at parque zoológico arruda câmara, joão pessoa, paraíba, brazil. the most common bacteria were staphylococcus sp. (14.74%), corynebacterium sp. (13.68%), escherichia coli (13.68%) and shigella sp.(11.58%), and the less common were citrobacter sp. (1.05%), klebsiella pneumonia ... | 2009 | 24031343 |
| the gut microbiota of larvae of rhynchophorus ferrugineus oliver (coleoptera: curculionidae). | the red palm weevil (rpw) rhynchophorus ferrugineus olivier (coleoptera: curculionidae) is one of the major pests of palms. the larvae bore into the palm trunk and feed on the palm tender tissues and sap, leading the host tree to death. the gut microbiota of insects plays a remarkable role in the host life and understanding the relationship dynamics between insects and their microbiota may improve the biological control of insect pests. the purpose of this study was to analyse the diversity of t ... | 2014 | 24884866 |
| implementation of a computerized decision support system to improve the appropriateness of antibiotic therapy using local microbiologic data. | a prospective quasi-experimental study was undertaken in 218 patients with suspicion of nosocomial infection hospitalized in a polyvalent icu where a new electronic device (gerb) has been designed for antibiotic prescriptions. two gerb-based applications were developed to provide local resistance maps (lrms) and preliminary microbiological reports with therapeutic recommendation (pmrtrs). both applications used the data in the laboratory information system of the microbiology department to repor ... | 2014 | 25197643 |