Publications

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de novo biosynthesis of biodiesel by escherichia coli in optimized fed-batch cultivation.biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (fames) and fatty acid ethyl esters (faees), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (tags). tags are mainly obtained from oilseed plants and microalgae. a sustainable supply of tags is a major bottleneck ...201121629774
Engineering yield and rate of reductive biotransformation in Escherichia coli by partial cyclization of the pentose phosphate pathway and PTS-independent glucose transport.Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral ß-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) to the chiral hydroxy ester (R)-methyl 3-hydroxybutyrate (MHB) using glucose as substrate for the generation of NADPH. Deletion of the phosphofructokinase gene pfkA almost doubled th ...201122002070
analysis of experimental errors in bioprocesses. 1. production of lactobionic acid and sorbitol using the gfor (glucose-fructose oxidoreductase) enzyme from permeabilized cells of zymomonas mobilis.the proper determination of experimental errors in bioprocesses can be very important because experimental errors can exert a major impact on the analysis of experimental results. despite this, the effect of experimental errors on the analysis of bioprocess data has been largely overlooked in the literature. for this reason, we performed detailed statistical analyses of experimental errors obtained during the production of lactobionic acid and sorbitol in a system utilizing as catalyst the gfor ...201121328074
adaptive evolution of escherichia coli inactivated in the phosphotransferase system operon improves co-utilization of xylose and glucose under anaerobic conditions.modification of the phosphoenolpyruvate/sugar phosphotransferase system (pts) has shown improvement in sugar coassimilation in escherichia coli production strains. however, in preliminary experiments under anaerobic conditions, e. coli strains with an inactive pts and carrying ploi1594, which encodes pyruvate decarboxylase and alcohol dehydrogenase from zymomonas mobilis, were unable to grow. these pts⁻ strains were previously evolved under aerobic conditions to grow rapidly in glucose (pts⁻ glu ...201120740380
conditioning of dilute-acid pretreated corn stover hydrolysate liquors by treatment with lime or ammonium hydroxide to improve conversion of sugars to ethanol.dilute-acid pretreatment of lignocellulosic biomass enhances the ability of enzymes to hydrolyze cellulose to glucose, but produces many toxic compounds that inhibit fermentation of sugars to ethanol. the objective of this study was to compare the effectiveness of treating hydrolysate liquor with ca(oh)2 and nh4oh for improving ethanol yields. corn stover was pretreated in a pilot-scale reactor and then the liquor fraction (hydrolysate) was extracted and treated with various amounts of ca(oh)2 o ...201120801647
electron transport and oxidative stress in zymomonas mobilis respiratory mutants.the ethanol-producing bacterium zymomonas mobilis is of great interest from a bioenergetic perspective because, although it has a very high respiratory capacity, the respiratory system does not appear to be primarily required for energy conservation. to investigate the regulation of respiratory genes and function of electron transport branches in z. mobilis, several mutants of the common wild-type strain zm6 (atcc 29191) were constructed and analyzed. mutant strains with a chloramphenicol-resist ...201222228443
Conceptual design of cost-effective and environmentally-friendly configurations for fuel ethanol production from sugarcane by knowledge-based process synthesis.In this work, the hierarchical decomposition methodology was used to conceptually design the production of fuel ethanol from sugarcane. The decomposition of the process into six levels of analysis was carried out. Several options of technological configurations were assessed in each level considering economic and environmental criteria. The most promising alternatives were chosen rejecting the ones with a least favorable performance. Aspen Plus was employed for simulation of each one of the tech ...201222137752
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