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production of lactobionic acid and sorbitol from lactose/fructose substrate using gfor/gl enzymes from zymomonas mobilis cells: a kinetic study.in this work, we have investigated the kinetics of the biotechnological production of lactobionic acid (lba) and sorbitol by the catalytic action of glucose-fructose oxidoreductase (gfor) and glucono-δ-lactonase (gl) enzymes. the cells of bacterium zymomonas mobilis atcc 29191 containing this enzymatic complex were submitted to permeabilization and reticulation procedures. the effect of the concentration of substrates on the rate of product formation using a mobilized cell system was investigate ...201122112407
Biotransformation of pineapple juice sugars into dietetic derivatives by using a cell free oxidoreductase from Zymomonas mobilis together with commercial invertase.An easy procedure for cell free biotransformation of pineapple juice sugars into dietetic derivatives was accomplished using a commercial invertase and an oxidoreductase from Zymomonas mobilis. First, pineapple juice sucrose was quantitatively converted into glucose and fructose by invertase, thus increasing the concentration of each monosaccharide in the original juice to almost twice. In a second step, glucose-fructose oxidoreductase (GFOR) transformed glucose into gluconolactone, and fructose ...201122112775
Biochemical and molecular characterization of NAD(+) -dependent isocitrate dehydrogenase from the ethanologenic bacterium Zymomonas mobilis.An isocitrate dehydrogenase from Zymomonas mobilis was overexpressed in Escherichia coli as a fused protein (ZmIDH). The molecular mass of recombinant ZmIDH, together with its 6× His partner, was estimated to be 74 kDa by gel filtration chromatography, suggesting a homodimeric structure. The purified recombinant ZmIDH displayed maximal activity at 55 °C, pH 8.0 with Mn(2+) and pH 8.5 with Mg(2+) . Heat inactivation studies showed that the recombinant ZmIDH was rapidly inactivated above 40 °C. I ...201122117777
Conceptual design of cost-effective and environmentally-friendly configurations for fuel ethanol production from sugarcane by knowledge-based process synthesis.In this work, the hierarchical decomposition methodology was used to conceptually design the production of fuel ethanol from sugarcane. The decomposition of the process into six levels of analysis was carried out. Several options of technological configurations were assessed in each level considering economic and environmental criteria. The most promising alternatives were chosen rejecting the ones with a least favorable performance. Aspen Plus was employed for simulation of each one of the tech ...201222137752
activation-independent cyclization of monoterpenoids.biosynthesis of cyclic monoterpenes (c(10)) generally requires cyclization of an activated linear precursor (geranyldiphosphate) by specific terpene cyclases. cyclic triterpenes (c(30)) on the other hand originate from the linear precursor squalene by the action of squalene-hopene cyclases (shcs) or oxidosqualene cyclases (oscs). here, we report on a novel terpene cyclase from zymomonas mobilis (zmo1548-shc) with the unique property to cyclize citronellal to isopulegol. to our knowledge zmo1548- ...201122156419
Fermentation of Reactive-Membrane-Extracted and Ammonium-Hydroxide-Conditioned Dilute-Acid-Pretreated Corn Stover.Acid-pretreated biomass contains various compounds (acetic acid, etc.) that are inhibitory to fermentative microorganisms. Removing or deactivating these compounds using detoxification methods such as overliming or ammonium hydroxide conditioning (AHC) improves sugar-to-ethanol yields. In this study, we treated the liquor fraction of dilute-acid-pretreated corn stover using AHC and a new reactive membrane extraction technique, both separately and in combination, and then the sugars in the treate ...201122161211
electron transport and oxidative stress in zymomonas mobilis respiratory mutants.the ethanol-producing bacterium zymomonas mobilis is of great interest from a bioenergetic perspective because, although it has a very high respiratory capacity, the respiratory system does not appear to be primarily required for energy conservation. to investigate the regulation of respiratory genes and function of electron transport branches in z. mobilis, several mutants of the common wild-type strain zm6 (atcc 29191) were constructed and analyzed. mutant strains with a chloramphenicol-resist ...201222228443
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