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isolation and identification of dibutyl phthalate-degrading bacteria from hydrospheres in tokyo.dibutyl phthalate (dbp) is used widely as a plasticizer and is thought to negatively affect various organisms. to isolate and investigate dbp-degrading bacteria from hydrospheres in tokyo, strains were selected on ynb medium containing dbp as the sole carbon source, and candidate strains were identified by zones of clearing around the colonies. degradation of dbp by the strains was subsequently measured with hplc, and bacterial identification was accomplished using 16s rdna sequences. nineteen s ...200919700919
a conserved hydrogen-bond network stabilizes the structure of beta class glutathione s-transferases.we identified a network of hydrogen bonds that is conserved in the structures of bacterial beta class glutathione s-transferases (gsts). it is formed by three residues: a serine, a histidine and a glutamate, together with a water molecule that links the serine with the histidine. this network connects the first helix of the n-terminal glutaredoxin-like domain with the last helix of the c-terminal gst-specific all helical domain. here we show that substitution of ochrobactrum anthropi gst his15 a ...200919289099
efficiency of the eps emulsifier produced by ochrobactrum anthropi in different hydrocarbon bioremediation assays.ochrobactrum anthropi strain ad2 was isolated from the waste water treatment plant of an oil refinery and was identified by analysis of the sequence of the gene encoding 16s rdna. this bacterium produced exopolysaccharides in glucose nutrient broth media supplemented with various hydrocarbons (n-octane, mineral light and heavy oils and crude oils). the exopolysaccharide ad2 (eps emulsifier) synthesized showed a wide range of emulsifying activity but none of them had surfactant activity. yield pr ...200818784947
isolation of functional single cells from environments using a micromanipulator: application to study denitrifying bacteria.we developed a novel method to isolate functionally active single cells from environmental samples and named it the functional single-cell (fsc) isolation method. this method is based on a combination of substrate-responsive direct viable counts, live-cell staining with 5-carboxyfluorescein diacetate acetoxymethyl ester, and micromanipulation followed by cultivation in a medium. to evaluate this method, we applied it to study a denitrifying community in rice paddy soil. similar denitrifier count ...201019936739
isolation of marine benzo[a]pyrene-degrading ochrobactrum sp. bap5 and proteins characterization.a bacterial strain bap5 with a relatively high degradation ability of benzo[a]pyrene (bap) was isolated from marine sediments of xiamen western sea, china and identified as ochrobactrum sp. according to 16s rrna gene sequence as well as biolog microbial identification system. strain bap5 could grow in mineral salt medium with 50 mg/l of bap and degrade about 20% bap after 30 d of incubation. ochrobactrum sp. bap5 was able to utilize other polycyclic aromatic hydrocarbons (pahs) (such as phenanth ...200920000001
stratified bacterial community in the bladder of the medicinal leech, hirudo verbana.most animals harbour symbiotic microorganisms inside their body, where intimate interactions occur between the partners. the medicinal leech, hirudo verbana, possesses 17 pairs of excretory bladders that harbour a large number of intracellular and extracellular symbiotic bacteria. in this study, we characterized the bladder symbionts using molecular phylogenetic analyses, transmission electron microscopy (tem) and fluorescence in situ hybridization (fish). restriction fragment length polymorphis ...200919678832
aidh, an alpha/beta-hydrolase fold family member from an ochrobactrum sp. strain, is a novel n-acylhomoserine lactonase.n-acylhomoserine lactones (ahls) are signaling molecules in many quorum-sensing (qs) systems that regulate interactions between various pathogenic bacteria and their hosts. quorum quenching by the enzymatic inactivation of ahls holds great promise in preventing and treating infections, and several such enzymes have been reported. in this study, we report the characterization of a novel ahl-degrading protein from the soil bacterium ochrobactrum sp. strain t63. this protein, termed aidh, shares no ...201020525860
nitrite reductase genes in halobenzoate degrading denitrifying bacteria.abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ...200319719666
isolation from ochrobactrum anthropi of a novel class ii 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.applying the genomic library construction process and colony screening, a novel aroa gene encoding 5-enopyruvylshikimate-3-phosphate synthase from ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.201020601515
characterization and phylogenetic analysis of biodemulsifier-producing bacteria.based on demulsification performance, twenty biodemulsifier-producing strains were isolated from various environmental sources. five of them achieved nearly or over 90% of emulsion breaking ratio within 24 h. with the aid of biochemical and physiological tests and 16s rdna analysis, these isolates were classified into eleven genera, in which six genera (brevibacillus sp., dietzia sp., ochrobactrum sp., pusillimonas sp., sphingopyxis sp. and achromobacter sp.) were firstly reported as demulsifyin ...201019720525
[ochrobactrum anthropi bacteremia in a child with cystic fibrosis]. 201019811858
ochrobactrum pituitosum sp. nov., isolated from an industrial environment.strain ccug 50899, a gram-negative, rod-shaped, non-spore-forming, motile bacterium isolated from industrial environment in sweden and tentatively assigned to the species ochrobactrum anthropi, was studied in order to clarify its taxonomic status. 16s rrna gene sequence similarities placed the strain in the genus ochrobactrum, sharing highest similarity with the type strains of ochrobactrum rhizosphaerae (99.3 %), ochrobactrum thiophenivorans (98.7 %), ochrobactrum pseudogrignonense (98.6 %) and ...201019651740
bioremediation of glyphosate-contaminated soils.based on the results of laboratory and field experiments, we performed a comprehensive assessment of the bioremediation efficiency of glyphosate-contaminated soddy-podzol soil. the selected bacterial strains achromobacter sp. kg 16 (vkm b-2534d) and ochrobactrum anthropi gpk 3 (vkm b-2554d) were used for the aerobic degradation of glyphosate. they demonstrated high viability in soil with the tenfold higher content of glyphosate than the recommended dose for the single in situ treatment of weeds. ...201020676632
electrochemical activation of nitrate reduction to nitrogen by ochrobactrum sp. g3-1 using a noncompartmented electrochemical bioreactor.a denitrification bacterium was isolated from riverbed soil and identified as ochrobactrum sp., whose specific enzymes for denitrification metabolism were biochemically assayed or confirmed with specific coding genes. the denitrification activity of strain g3-1 was proportional to glucose/nitrate balance, which was consistent with the theoretical balance (0.5). the modified graphite felt cathode with neutral red, which functions as a solid electron mediator, enhanced the electron transfer from e ...200919734723
glyphosate bioavailability in soil.biodegradation of glyphosate in sod-podzol soil by both the indigenous micro flora and the introduced strain ochrobactrum anthropi gpk 3 was studied with respect to its sorption and mobility. the experiments were carried out in columns simulating the vertical soil profile. soil samples studied were taken from soil horizons 0-10, 10-20, and 20-30 cm deep. it was found out that the most of the herbicide (up to 84%) was adsorbed by soil during the first 24 h; the rest (16%) remained in the soluble ...201019902365
bacterial bloodstream infections in hiv-infected adults attending a lagos teaching hospital.an investigation was carried out during october 2005-september 2006 to determine the prevalence of bloodstream infections in patients attending the outpatient department of the hiv/aids clinic at the lagos university teaching hospital in nigeria. two hundred and one patients--86 males and 115 females--aged 14-65 years were recruited for the study. serological diagnosis was carried out on them to confirm their hiv status. their cd4 counts were done using the micromagnetic bead method. twenty ml o ...201020824974
characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses.diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ...201120859703
beta-aminopeptidase-catalyzed biotransformations of beta(2)-dipeptides: kinetic resolution and enzymatic coupling.we have previously shown that the beta-aminopeptidases bapa from sphingosinicella xenopeptidilytica and dmpa from ochrobactrum anthropi can catalyze reactions with non-natural beta(3)-peptides and beta(3)-amino acid amides. here we report that these exceptional enzymes are also able to utilize synthetic dipeptides with n-terminal beta(2)-amino acid residues as substrates under aqueous conditions. the suitability of a beta(2)-peptide as a substrate for bapa or dmpa was strongly dependent on the s ...201020340152
ochrobactrum anthropi peritonitis in an immunocompetent capd patient. 200919910572
different physiological responses to chromate and dichromate in the chromium resistant and reducing strain ochrobactrum tritici 5bvl1.studies of cr(vi) toxicity are generally performed using chromate salts in solution, both when studying the effects on prokaryotes and eukaryotes. some studies on human carcinogenesis and toxicology on bacteria were done using dichromate, but comparison with chromate was never reported before, and dichromate existence was never taken into consideration and usually overlooked. this paper studied comparatively the effect of dichromate and chromate on the physiology of ochrobactrum tritici strain 5 ...201020390438
"in situ" phytostabilisation of heavy metal polluted soils using lupinus luteus inoculated with metal resistant plant-growth promoting rhizobacteria.the aim of this work is the evaluation of metal phytostabilisation potential of lupinus luteus inoculated with bradyrhizobium sp. 750 and heavy metal resistant pgprs (plant-growth promoting rhizobacteria), for in situ reclamation of multi-metal contaminated soil after a mine spill. yellow lupines accumulated heavy metals mainly in roots (cu, cd and especially pb were poorly translocated to shoots). this indicates a potential use of this plant in metal phytostabilisation. furthermore, as accumula ...201020056325
[sorption and microbial degradation of glyphosphate in soil suspensions].sorption and microbial destruction of glyphosphate, the active agent of the herbicide groundbio, in suspensions of sod-podzolic and gray forest soils has been studied. according to the values of the adsorptive capacity (3560 and 8200 mg/kg, respectively) and the freundlich constants (kf, 15.6 and 18.7, respectively), these soils had a relatively high sorption capacity as related to the herbicide. sorbed glyphosphate is represented by extractable and bound (inextractable) fractions. after long-te ...200920067150
identification of an unusual brucella strain (bo2) from a lung biopsy in a 52 year-old patient with chronic destructive pneumonia.brucellosis is primarily a zoonotic disease caused by brucella species. there are currently ten brucella spp. including the recently identified novel b. inopinata sp. isolated from a wound associated with a breast implant infection. in this study we report on the identification of an unusual brucella-like strain (bo2) isolated from a lung biopsy in a 52-year-old patient in australia with a clinical history of chronic destructive pneumonia.201020105296
genomic comparisons of brucella spp. and closely related bacteria using base compositional and proteome based methods.classification of bacteria within the genus brucella has been difficult due in part to considerable genomic homogeneity between the different species and biovars, in spite of clear differences in phenotypes. therefore, many different methods have been used to assess brucella taxonomy. in the current work, we examine 32 sequenced genomes from genus brucella representing the six classical species, as well as more recently described species, using bioinformatical methods. comparisons were made at t ...201020707916
tightly regulated expression vectors for ochrobactrum anthropi.genetic studies of ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. a tightly regulated gene-expression system was developed for o. anthropi using the laci(q) gene and a re-engineered coliphage t5 promoter containing completely symmetrical dna segment that binds more efficiently to the lactose repressor. the beta-galactosidase activity was increased 57-fold when the expressi ...201020237980
cloning of the chlorothalonil-degrading gene cluster and evidence of its horizontal transfer.strain ochrobactrum lupine tp-d1 was found to degrade chlorothalonil (tpn) to 4-hydroxy-chlorothalonil (tpn-oh). to clone the related degrading gene, genomic library of tp-d1 was constructed using escherichia coli dh10b and two positive clones 889 and 838 were gained. however, no plasmid was detected in clone 889. and in clone 838, a 3494 bp fragment was cloned which contains a 984 bp hydrolytic dehalogenase (chd) gene and a 1926 bp insertion element is-olup. the insertion element contains a tra ...201021127873
peritonitis in type 2 diabetes mellitus due to ochrobactrum anthropi complicating automated peritoneal dialysis.epidemiology data predict that by the year 2025, diabetes will affect about 380 million people worldwide with a significant increase in patients with chronic renal disease progressing to hemodialysis. diabetes-related peripheral vascular disease is a major risk factor for vascular access failure in patients on extracorporeal hemodialysis. although peritoneal dialysis is a valid option for diabetics, peritonitis is still a main complication for these patients. we report the case of a 71-year-old ...201020563608
ribosomal rna sequence analysis of brucella infection misidentified as ochrobactrum anthropi infection.a brucella isolate was identified from purulent material collected during a hip surgery. two previous blood cultures from the same patient yielded ochrobactrum anthropi. after rrna sequencing, all the isolates were identified as brucella species and subsequently serotyped as brucella suis. misidentification of brucella species remains a problem with bacterial identification systems.201121209167
bacterial genome chimaerism and the origin of mitochondria.many studies have sought to determine the origin and evolution of mitochondria. although the alphaproteobacteria are thought to be the closest relatives of the mitochondrial progenitor, there is dispute as to what its particular sister group is. some have argued that mitochondria originated from ancestors of the order rickettsiales, or more specifically of the rickettsiaceae family, while others believe that ancestors of the family rhodospirillaceae are also equally likely the progenitors. to re ...201121217797
ochrobactrum ciceri sp. nov., isolated from nodules of cicer arietinum.a gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, ca-34(t), was isolated from nodules of chickpea (cicer arietinum) in pakistan and studied for its taxonomic affiliation. the almost full-length 16s rrna gene sequence showed highest similarities to those of strains of the genus ochrobactrum. based on results of maldi-tof ms and 16s rrna gene sequence similarity (98.6 %), strain ca-34(t) and ochrobactrum intermedium lmg 3301(t) are phylogenetic neighbours; the two ...201019684324
brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis.brucellosis is a zoonotic infection transmitted from animals to humans by the ingestion of infected food products, direct contact with an infected animal or inhalation of aerosols. the last method is remarkably efficient given the relatively low concentration of organisms (10 - 100 bacteria) needed to establish infection in humans, and has brought renewed attention to this old disease. brucella is a facultative intracellular pathogen that has the ability to survive and multiply in the phagocytes ...201021346896
application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents.a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ...201121419572
arsenic-resistant bacteria associated with roots of the wild cirsium arvense (l.) plant from an arsenic polluted soil, and screening of potential plant growth-promoting characteristics.a rhizobacterial community, associated with the roots of wild thistle cirsium arvense (l.) growing in an arsenic polluted soil, was studied by fluorescence in situ hybridization (fish) analysis in conjunction with cultivation-based methods. in the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative picture obtained by fish analysis of the main phylogenetic bacterial groups was similar and was predominantly comprised of alphaproteobacteria, betaproteobacteria, and gammaproteo ...201020303688
long-term hg pollution-induced structural shifts of bacterial community in the terrestrial isopod (porcellio scaber) gut.in previous studies we detected lower species richness and lower hg sensitivity of the bacteria present in egested guts of porcellio scaber (crustacea, isopoda) from chronically hg polluted than from unpolluted environment. basis for such results were further investigated by sequencing of 16s rrna genes of mercury-resistant (hgr) isolates and clone libraries. we observed up to 385 times higher numbers of hgr bacteria in guts of animals from polluted than from unpolluted environment. the majority ...201020724045
new approaches to identification and activity estimation of glyphosate degradation enzymes.we propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (n-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation in vivo and in vitro. using the developed approach we show that glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains: in ochrobactrum anthropi gpk3 the initial cleavage reaction is catalyzed by glyphosate-oxidoreductase wit ...201121639854
oil phytoremediation potential of hypersaline coasts of the arabian gulf using rhizosphere technology.the rhizosphere and phyllosphere of the halophyte halonemum strobilaceum naturally inhabiting hypersaline coastal areas of the arabian gulf harbor up to 8.1 x 10(4)g(-1) and 3 x 10(2)g(-1), respectively, of extremely halophilic oil-utilizing microorganisms. such organisms were 14- to 38-fold more frequent in the rhizosphere than in the plant-free soil. frequent genera in the rhizosphere were affiliated to the archaea halobacterium sp. and halococcus sp., the firmicute brevibacillus borstenlensis ...201020303746
distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria ochrobactrum anthropi and achromobacter sp.bacterial strains capable of utilizing methylphosphonic acid (mp) or glyphosate (gp) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. the strains isolated from mp-contaminated soils grew on mp and failed to grow on gp. one group of the isolates from gp-contaminated soils grew only on mp, while the other one grew on mp and gp. strains achromobacter sp. mps 12 (vkm b-2694), mp degraders group, and ochrobactrum anthropi gpk 3 (vkm b-2554d), gp d ...201121789492
[biodegradation of nicotine in tobacco extracts for making reconstituted tobacco by strain dn2].the purpose of the study is to use o. intermedium dn2 to degrade nicotine in tobacco extracts for making reconstituted tobacco. firstly, we studied the effects of various factors on degradation of nicotine in the extracts by strain dn2. when we added 0.1% yeast extract into the extracts, adjusted its ph value to 7.0 by ammonia solution, inoculated 15% cultures and maintained fermentation temperature of 30 degrees c, the degradation rate of nicotine by strain dn2 was the fastest. furthmore, under ...200919777819
degradation of di-n-butyl phthalate by newly isolated ochrobactrum sp.a gram negative isolate designated jdc-41 was obtained from river sludge using mixtures of phthalate esters as the sole source and energy. the isolate was identified as ochrobactrum sp. based on its 16s rrna gene sequence. over 87% of supplied di-n-butyl phthalate (dbp) was degraded by jdc-41 in a ph neutral mineral salts medium at 30 degrees c within 48 h. increased dbp (50-500 mg/l) in the culture correspondingly increased degradation half-life from 3.83 to 18.12 h. dbp induced cells more rapi ...201020658278
[ochrobactrum anthropi and polymicrobial peritonitis in peritoneal dialysis: a resistance predictor]. 201020882099
bacterial community associated with healthy and diseased reef coral mussismilia hispida from eastern brazil.in order to characterize the bacterial community diversity associated to mucus of the coral mussismilia hispida, four 16s rdna libraries were constructed and 400 clones from each library were analyzed from two healthy colonies, one diseased colony and the surrounding water. nine bacterial phyla were identified in healthy m. hispida, with a dominance of proteobacteria, actinobacteria, acidobacteria, lentisphaerae, and nitrospira. the most commonly found species were related to the genera azospiri ...201020352207
[catheter- associated bacteremia caused by ochrobactrum anthropi].ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as achromobacter sp or cdc group vd. it has been isolated from the environment and from infections in usually immunocompromised human beings. the documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. here it is presente ...200920053608
characterization of a cd(2+)-resistant strain of ochrobactrum sp. isolated from slag disposal site of an iron and steel factory.a cadmium-resistant bacterium designated as cdsp9 was isolated from the slag disposal site of iisco, burnpur, west bengal, india. the isolate was identified as ochrobactrum sp. on the basis of 16s rdna sequence-based molecular phylogenetic approach and phenotypic characteristics. it is a gram negative, short rod (0.5-1.0 micro), aerobic bacterium, growing well in lb medium between temperatures 10-42 degrees c, ph 6.0-9.0, and between 2 and 6% nacl. the most preferred nitrogen and carbon sources ...201020091408
molecular determinants for substrate selectivity of ω-transaminases.ω-transaminase (ω-ta) is an industrially important enzyme for production of chiral amines. about 20 (s)-specific ω-tas known to date show remarkably similar substrate selectivity characterized by stringent steric constraint precluding entry of a substituent larger than an ethyl group in the small binding pocket (s) and dual recognition of an aromatic substituent as well as a carboxylate group in the large pocket (l). the strictly defined substrate selectivity of the available ω-tas remains a lim ...201121983703
identification and characterization of the chromium (vi) responding protein from a newly isolated ochrobactrum anthropi cts-325.a gram-negative, chromium(vi) tolerant and reductive strain cts-325, isolated from a chinese chromate plant, was identified as ochrobactrum anthropi based on its biochemical properties and 16s rdna sequence analysis. it was able to tolerate up to 10 mmol/l cr(vi) and completely reduce 1 mmol/l cr(vi) to cr(iii) within 48 h. when the strain cts-325 was induced with cr(vi), a protein increased significantly in the whole cell proteins. liquid chromatography tandem mass spectrometry (lc-ms/ms) analy ...200920131597
hydrolytic dechlorination of chlorothalonil by ochrobactrum sp. ctn-11 isolated from a chlorothalonil-contaminated soil.a bacterial strain, designated as ctn-11, capable of degrading chlorothalonil (ctn), was isolated from a chlorothalonil-contaminated soil in china. based on the morphological, biochemical characteristics and comparative analysis of the 16s rrna genes, strain ctn-11 was identified as ochrobactrum sp. strain ctn-11 could degrade 50 mg l(-1) ctn to a non-detectable level within 48 h, and efficiently degrade ctn in a relatively broad range of temperatures from 20 to 40 degrees c and initial ph value ...201020148249
differences in two-component signal transduction proteins among the genus brucella: implications for host preference and pathogenesis.two-component systems (tcss) are the predominant bacterial signal transduction mechanisms. species of the genus brucella are genetically highly related and differ mainly in mammalian host adaptation and pathogenesis. in this study, tcs proteins encoded in the available genome sequences of brucella species have been identified using bioinformatic methods. all the brucella species share an identical set of tcs proteins, and the number of tcs proteins in the closely related opportunistic human path ...201020153589
biliary sepsis caused by ochrobactrum anthropi.ochrobactrum anthropi is an emerging pathogen in immunocompromised patients, with the majority of human cases being central venous catheter-related infections. in contrast, o. anthropi-related biliary sepsis is much rare. herein we report the clinical and microbiological characteristics of o. anthropi-related biliary sepsis in order to increase awareness of the potential role of o. anthropi in this infection. further extensive epidemiologic studies should be carried out to ascertain the etiologi ...201021099098
ochrobactrum daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.a gram-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated mj11t, was isolated from sludge of a leachate treatment plant in daejeon (south korea) and was characterized taxonomically by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj11t belongs to the family brucellaceae, class alphaproteobacteria, and is related most closely to ochrobactrum ciceri ca-34t (97.9 % sequence similarity) and ochrobactrum pituitosum ccug 50899t ( ...201021169456
antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria).the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ...201122095134
Generation of continuous packed bed reactor with PVA-alginate blend immobilized Ochrobactrum sp. DGVK1 cells for effective removal of N,N-dimethylformamide from industrial effluents.Effective removal of dimethylformamide (DMF), the organic solvent found in industrial effluents of textile and pharma industries, was demonstrated by using free and immobilized cells of Ochrobactrum sp. DGVK1, a soil isolate capable of utilizing DMF as a sole source of carbon, nitrogen. The free cells have efficiently removed DMF from culture media and effluents, only when DMF concentration was less than 1% (v/v). Entrapment of cells either in alginate or in polyvinyl alcohol (PVA) failed to inc ...201222079508
biodegradation of beta-cypermethrin and 3-phenoxybenzoic acid by a novel ochrobactrum lupini dg-s-01.a newly isolated bacterium dg-s-01 from activated sludge utilized beta-cypermethrin (beta-cp) and its major metabolite 3-phenoxybenzoic acid (3-pba) as sole carbon and energy source for growth in mineral salt medium (msm). based on the morphology, physio-biochemical characteristics, and 16s rdna sequence analysis, dg-s-01 was identified as ochrobactrum lupini. dg-s-01 effectively degraded beta-cp with total inocula biomass a(590 nm) = 0.1-0.8, at 20-40 °c, ph 5-9, initial beta-cp 50-400 mg l(-1) ...201121282001
chromium resistance strategies and toxicity: what makes ochrobactrum tritici 5bvl1 a strain highly resistant.large-scale industrial use of chromium (cr) resulted in widespread environmental contamination with hexavalent chromium (cr(vi)). the ability of microorganisms to survive in these environments and detoxify chromate requires the presence of specific resistance systems. several cr(vi) resistant species, belonging to a variety of genera, have been isolated in recent years. ochrobactrum tritici strain 5bvl1 is a model for a highly cr(vi)-resistant and reducing microorganism, with different strategie ...201121472416
role of nutrients in the utilization of polycyclic aromatic hydrocarbons by halotolerant bacterial strain.a halotolerant bacterial strain va1 isolated from marine environment was studied for its ability to utilize polycylic aromatic hydrocarbons (pahs) under saline condition. anthracene and pyrene were used as representatives for the utilization of pah by the bacterial strain. glucose and sodium citrate were used as additional carbon sources to enhance the pah utilization. the strain va1 was able to utilize anthracene (73%) and pyrene (66%) without any additional substrate. in the presence of additi ...201121517002
Intra-species biodiversity of the genetically homologous species Brucella microti.Brucellosis is one of the major bacterial zoonoses worldwide. In the last decade, an increasing number of atypical Brucella strains and species have been described. Brucella microti in particular attracted attention because this species not only infects mammalian hosts but also persists in soil. An environmental reservoir may pose a new public health risk leading to the re-emergence of brucellosis. In a polyphasic approach, comprising conventional microbiological, extensive biochemical and molec ...201122210211
bioremediation of cr(vi) and immobilization as cr(iii) by ochrobactrum anthropi.bioremediation of cr(vi) through reduction relies on the notion that the produced cr(iii) may be precipitated or efficiently immobilized. however, recent reports suggest that soluble organo-cr(iii) complexes are present in various chromate-reducing bacterial systems. this work was designed to explore the factors that affect the immobilization of cr(iii) in the ochrobactrum anthropi system. x-ray absorption fine structure analysis on the cell debris clearly verified that coordination of cr(iii) o ...201020608725
1,3-propanediol production potential of clostridium saccharobutylicum nrrl b-643.owing to the significant interest in biofuel production in the form of biodiesel, vast amount of glycerol as a waste product is produced all over the world. among the economically viable and ecologically acceptable solutions for the safe disposal of this waste, biotechnological conversion of glycerol into a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) seems to be very promising. in this study, 1,3-pdo production potential of clostridium saccharobutylicum nrrl b-643 was stud ...201020647065
16s rdna-based phylogeny of non-symbiotic bacteria of entorno-pathogenic nematodes from infected insect cadavers.using 16s rdna gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (epns) (steinernema sp. and heterorhabditis sp.) were isolated and identified from infected insect cadavers {galleria mellonella larvae) after 48-hour post infections. sequence similarity analysis revealed that the strains srk3, srk4 and srk5 belong to ochrobactrum cytisi, schineria larvae and ochrobactrum anthropi, respectively. the isolates o. anthropi and s. larvae were f ...201121802047
complex prokaryotic genome structure: rapid evolution of chromosome ii.although many bacteria with two chromosomes have been sequenced, the roles of such complex genome structuring are still unclear. to uncover levels of chromosome i (ci) and chromosome ii (cii) sequence divergence, mauve 2.2.0 was used to align the ci- and cii-specific sequences of bacteria with complex genome structuring in two sets of comparisons: the first set was conducted among the ci and cii of bacterial strains of the same species, while the second set was conducted among the ci and cii of ...201020924417
biodegradation of polycyclic aromatic hydrocarbons by a halotolerant bacterial strain ochrobactrum sp. va1.polycyclic aromatic hydrocarbons (pahs) are ubiquitous pollutants in the environment and are derived from both man-made and natural resources. the present study is focused on the degradation of pahs by a halotolerant bacterial strain under saline conditions. the bacterial strain va1 was isolated from a pah-degrading consortium that was enriched from marine water samples that were collected from different sites at chennai, india. in the present study, a clearing zone formed on pah-amended mineral ...201020934193
ochrobactrum pecoris sp. nov., isolated from farm animals.two gram-negative, rod-shaped, non-spore-forming strains, 08rb2639(t) and 08rb2781-1 isolated from sheep (ovis aries) and boar (sus scrofa domestica), respectively, were studied for their taxonomic position. by 16s rrna gene sequencing, these strains revealed identical sequences and were shown to belong to the alphaproteobacteria with 97.8 % sequence similarity to ochrobactrum rhizosphaerae pr17(t), ochrobactrum pituitosum ccug 50899(t) , ochrobactrum tritici scii24(t) and ochrobactrum haematoph ...201020952542
multilocus sequence typing supports the hypothesis that ochrobactrum anthropi displays a human-associated subpopulation.ochrobactrum anthropi is a versatile bacterial species with strains living in very diverse habitats. it is increasingly recognized as opportunistic pathogen in hospitalized patients. the population biology of the species particularly with regard to the characteristics of the human isolates is being investigated. to address this issue, we proposed a polyphasic approach consisting in multi-locus sequence typing (mlst), multi-locus phylogeny, genomic-based fingerprinting by pulsed-field gel electro ...200920021660
degradation of phenanthrene via meta-cleavage of 2-hydroxy-1-naphthoic acid by ochrobactrum sp. strain pwtjd.the present study describes the assimilation of phenanthrene by an aerobic bacterium, ochrobactrum sp. strain pwtjd, isolated from municipal waste-contaminated soil sample utilizing phenanthrene as a sole source of carbon and energy. the isolate was identified as ochrobactrum sp. based on the morphological, nutritional and biochemical characteristics as well as 16s rrna gene sequence analysis. a combination of chromatographic analyses, oxygen uptake assay and enzymatic studies confirmed the degr ...201020964703
enhanced thermostability of methyl parathion hydrolase from ochrobactrum sp. m231 by rational engineering of a glycine to proline mutation.protein thermostability can be increased by some glycine to proline mutations in a target protein. however, not all glycine to proline mutations can improve protein thermostability, and this method is suitable only at carefully selected mutation sites that can accommodate structural stabilization. in this study, homology modeling and molecular dynamics simulations were used to select appropriate glycine to proline mutations to improve protein thermostability, and the effect of the selected mutat ...201020977676
erratum to: peritonitis in type 2 diabetes mellitus due to ochrobactrum anthropi complicating automated peritoneal dialysis. 201021061136
comparison of exoelectrogenic bacteria detected using two different methods: u-tube microbial fuel cell and plating method.in a microbial fuel cell (mfc), exoelectrogens, which transfer electrons to the electrode, have been regarded as a key factor for electricity generation. in this study, u-tube mfc and plating methods were used to isolate exoelectrogens from the anode of an mfc. disparate microorganisms were identified depending on isolation methods, despite the use of an identical source. denaturing gel gradient electrophoresis (dgge) analysis showed that certain microorganisms became dominant in the u-tube mfc. ...201122129603
enzymatic properties of the glycine d-alanine aminopeptidase of aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji).the gdaa gene encoding s12 family glycine-d: -alanine aminopeptidase (gdaa) was found in the industrial fungus aspergillus oryzae. gdaa shares 43% amino acid sequence identity with the d: -aminopeptidase of the gram-negative bacterium ochrobactrum anthropi. gdaa purified from an a. oryzae gdaa-overexpressing strain exhibited high d: -stereospecificity and efficiently released n-terminal glycine and d: -alanine of substrates in a highly specific manner. the optimum ph and temperature were 8 to 9 ...201122005737
antifouling activities against colonizer marine bacteria of extracts from marine invertebrates collected in the colombian caribbean sea and on the brazilian coast (santa catarina).the growth inhibition of 12 native marine bacteria isolated from aplysina sponge surfaces, the shell of a bivalve, and phytagel immersed for 48 h in sea water were used as indicator of the antifouling activity of the extracts of 39 marine organisms (octocorals, sponges, algae, and zoanthid) collected in the colombian caribbean sea and on the brazilian coast (santa catarina). gram-negative bacteria represented 75% of the isolates; identified strains belonged to oceanobacillus iheyensis, ochrobact ...201122191218
Improvement of glyphosate resistance through concurrent mutations in three amino acids of the Ochrobactrum 5-enopyruvylshikimate-3-phosphate synthase.A mutant of 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified after four rounds of DNA shuffling and screening. Its ability to restore the growth of the mutant ER2799 cell on an M9 minimal medium containing 300 mM glyphosate led to its identification. The mutant had mutations in seven amino acids: E145G, N163H, N267S, P318R, M377V, M425T, and P438L. Among these mutations, N267S, P318R, and M425T have never been previously reported as important residues for glyp ...201121948846
Distribution of microbial biomass and the potential for anaerobic respiration in Hanford Site 300 Area subsurface sediment.Subsurface sediments were recovered from a 52 m deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the oxic coarse-grained Hanford formation (9-17.4 m), the oxic fine-grained upper Ringold Formation (17.7-18.1 m), and the reduced Ringold Formation (18.3-52m). Microbial biomass (measu ...201122138990
genetic and functional characterization of a yet-unclassified rhizobial dtr (dna-transfer-and-replication) region from a ubiquitous plasmid conjugal system present in sinorhizobium meliloti, in sinorhizobium medicae, and in other nonrhizobial gram-negative bacteria.rhizobia are gram-negative bacteria that live in soils and associate with leguminous plants to establish nitrogen-fixing symbioses. the ability of these bacteria to undergo horizontal gene transfer (hgt) is thought to be one of the main features to explain both the origin of their symbiotic life-style and the plasticity and dynamics of their genomes. in our laboratory we have previously characterized at the species level the non-psym plasmid mobilome in sinorhizobium meliloti, the symbiont of me ...201222233546
isolation of estrogen-degrading bacteria from an activated sludge bioreactor treating swine waste, including a strain that converts estrone to ß-estradiol.an estrogen-degrading bacterial consortium from a swine wastewater biotreatment was enriched in the presence of low concentrations (1 mg/l) of estrone (e1), 17ß-estradiol (ße2), and equol (eqo) as sole carbon sources. the consortium removed 99% ± 1% of these three estrogens in 48 h. estrogen removal occurred even in the presence of an ammonia monooxygenase inhibitor, suggesting that nitrifiers are not involved. five strains showing estrogen-metabolizing activity were isolated from the consortium ...201121770814
one-step process for debromination and aerobic mineralization of tetrabromobisphenol-a by a novel ochrobactrum sp. t isolated from an e-waste recycling site.a novel bacterium, ochrobactrum sp. t, capable of simultaneous debromination and aerobic mineralization of tetrabromobisphenol-a (tbbpa), was isolated from a sludge sample collected from an electronic-waste recycling site. the bacterium exhibited maximal debrominase activity at ph 6.5, 35°c, and 200rpm in luria-bertani culture medium. initial tbbpa concentration and ph had more significant effects on degradation efficiency than those of temperature and inoculum size. degradation and debrominatio ...201121764300
isolation and characterization of lead-tolerant ochrobactrum intermedium and its role in enhancing lead accumulation by eucalyptus camaldulensis.in this study, the potential of rhizospheric bacteria in promoting the growth and pb accumulation by the woody plant eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. three pb-tolerant bacteria were isolated from the rhizosphere of e. camaldulensis grown in pb-contaminated soils in the bo ngam pb mine, thailand. based on analysis of partial 16s rrna gene sequence, the three isolates were identified as microbacterium paraoxydans bn-2, ochrobactrum intermedi ...201121764101
genome of ochrobactrum anthropi atcc 49188t, a versatile opportunistic pathogen and symbiont of several eukaryotic hosts.ochrobactrum anthropi is a common soil alphaproteobacterium that colonizes a wide spectrum of organisms and is being increasingly recognized as an opportunistic human pathogen. potentially life-threatening infections, such as endocarditis, are included in the list of reported o. anthropi infections. these reports, together with the scant number of studies and the organism's phylogenetic proximity to the highly pathogenic brucellae, make o. anthropi an attractive model of bacterial pathogenicity. ...201121685287
improving the thermostability of a methyl parathion hydrolase by adding the ionic bond on protein surface.the thermostability of the methyl parathion hydrolase (mph_och) from ochrobactrum sp. m231 was improved using site-directed mutagenesis. two prolines (pro76 and pro78) located on the protein surface were selected for mutations after inspection of the sequence alignment of mph_och and ophc2, a thermostable organophosphorus hydrolase from pseudomonas pseudoalcaligenes c2-1. the temperature of the double-point mutant (p76d/p78k) at which the mutant lost 50% of its activity (t50) was approximately 6 ...201121728027
Recurrent Ochrobactrum anthropi and Shewanella putrefaciens bloodstream infection complicating hemodialysis.Bloodstream infections (BSIs) are common in hemodialysis, especially when the access is a catheter. These infections are more commonly gram-positive bacteria or gram-negative bacilli and on some occasions, fungi. Ochrobactrum anthropi and Shewanella putrefaciens are ubiquitous hydrophilic gram-negative bacilli. There have been three cases of O.?anthropi BSI reported in hemodialysis patients (one from the United States and two from Vienna) and two cases of S.?putrefaciens BSI in hemodialysis pati ...201122099546
isolation and characterization of ochrobactrum anthropi and ochrobactrum pecoris from caecal content of commercial turkeys.two gram negative, micro-aerophilic, non-motile and non-spore-forming coccoid bacteria were isolated from female turkey caecal samples collected from a slaughterhouse. the biochemical reaction profiles (api 20 e and api 20 ne) typed both strains as ochrobactrum anthropi. on the basis of 16s rrna gene and reca gene sequence similarities the strains were identified as o. anthropi and ochrobactrum pecoris, respectively. both strains were highly resistant against beta-lactam antibiotics, chloramphen ...201121955448
hessian fly-associated bacteria: transmission, essentiality, and composition.plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ...201121858016
environmental microbiota represents a natural reservoir for dissemination of clinically relevant metallo-beta-lactamases.a total of 10 metallo-β-lactamase-producing isolates of six different species, including brevundimonas diminuta (n = 3), rhizobium radiobacter (n = 2), pseudomonas monteilii (n = 1), pseudomonas aeruginosa (n = 2), ochrobactrum anthropi (n = 1), and enterobacter ludwigii (n = 1), were detected in the sewage water of a hospital. the presence of bla(vim-13) associated with a tn1721-class 1 integron structure was detected in all but one of the isolates (e. ludwigii, which produced vim-2), and in tw ...201121859934
metal-induced phosphate extracellular nanoparticulate formation in ochrobactrum tritici 5bvl1.hexavalent chromium (cr(vi)) is a toxic environmental contaminant which detoxification consists in reduction to cr(iii). in this work, the cr(vi)-resistant and reducing ochrobactrum tritici 5bvl1 produced phosphate nanoparticles upon exposure to cr(vi) and fe(iii), effectively removing chromium from solution. under cr(vi) stress, higher siderophore production by strain 5bvl1 was observed. cr(vi) toxicity was decreased in presence of fe(iii), increasing the growth and cr(vi)-reduction rates in ce ...201122018866
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