TitleAbstractYear(sorted ascending)
establishment of a gene expression system in ochrobactrum anthropi.genetic studies of ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. we constructed a set of vectors containing several promoters and a his tag fusion in the n terminus to facilitate protein detection and purification. the new vectors should significantly enhance the genetic manipulation and characterization of o. anthropi.200617021239
alkane utilization by rhodococcus strain ntu-1 alone and in its natural association with bacillus fusiformis l-1 and ochrobactrum sp.linear (n-hexadecane) and branched (pristane) alkanes were degraded by a mixed culture isolated from an oil-contaminated field. the degradation was accompanied by formation of biofloccules. the culture was composed of rhodococcus strain ntu-1, bacillus fusiformis l-1, and ochrobactrum sp. rhodococcus strain ntu-1 carried out the degradation of the alkane via a hydroxylase. bacillus fusiformis l-1 and ochrobactrum sp. did not degrade the alkanes but aided the flocculation by forming more rigid ba ...200617022676
ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes.the use of two-phase centrifugal decanters has been widely adopted in the olive oil extraction industry in order to reduce the huge quantities of wastewaters produced during the traditional three-phase extraction process. the resulting sludge-like byproduct, widely known as "alpeorujo", has a ph of 4-6, low water activity (a(w)) and high phytotoxicity. addition of ca(oh)(2) to alpeorujo, which is commonly performed at the olive oil mill to handle disposal problems related to acidic ph and odor e ...200616307869
biodegradation kinetics of the nitramine explosive cl-20 in soil and microbial cultures.the cyclic nitramine explosive cl-20 (c(6)h(6)n(12)o(12), 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12 -hexaazaisowurtzitane) is a relatively new energetic compound which could be a persistent organic pollutant. to follow its biodegradation dynamics, cl-20 was added to soil alone or together with organic co-substrates and n-source and incubated under oxic and anoxic conditions. without co-substrates, the cl-20 degradation was detectable only under anoxic conditions. the highest degradation rate was fou ...200717091356
biodegradation of nonylphenol in soil.we investigated the effects of various factors (brij 30, brij 35, yeast extract, hydrogen peroxide and compost) on the aerobic degradation of nonylphenol (np) in soil and characterized the structure of the microbial community in that soil. residues of np were measured using gas chromatography-mass spectrometry (gc-ms) and a change of microbial communities was demonstrated using denaturing gradient gel electrophoresis (dgge). the results showed that taichung sandy clay loam had higher np degradat ...200717092543
high-level heterologous gene expression in ochrobactrum anthropi using an a-rich up element.dna regions that flank a gene's promoter play an important role in determining transcription efficiency by interacting with the carboxy-terminal domain of rna polymerase alpha-subunit. we placed an adenine-rich upstream element (up) between -38 and -59 of the core trc promoter to enhance gene expression in ochrobactrum anthropi up to 66-fold. the high level of expression achieved by the up element and the n-terminus fusion of a 6xhis epitope tag facilitated detection and purification of heterolo ...200716944128
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
biodegradation of nicotine from tobacco waste extract by ochrobactrum intermedium dn2.ochrobactrum intermedium dn2 was used to degrade nicotine in tobacco waste extracts. the optimal temperature and ph of nicotine degradation by strain dn2 was 30-37 degrees c and 7.0, respectively. under these optimal conditions, the average degradation rate of nicotine in a 30l fed-batch culture was 140.5 mg 1(-1) h(-1). the results of this study indicate that strain dn2 may be useful for reducing the nicotine content of reconstituted tobacco.200717333093
ochrobactrum cytisi sp. nov., isolated from nodules of cytisus scoparius in spain.two strains named esc1(t) and esc5 were isolated from nodules of cytisus scoparius growing in a spanish soil. phylogenetic analysis of the 16s rrna gene showed that these strains belong to the genus ochrobactrum, their closest relatives being ochrobactrum anthropi and ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. despite this high similarity, the results of dna-dna hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a ...200717392207
influences of metals on kinetics of methyl tert-butyl ether biodegradation by ochrobactrum cytisi.the influence of zinc, manganese, and nickel on the degradation of mtbe (methyl tert-butyl ether), by an aerobic mtbe-degrading strain, ochrobactrum cytisi, were investigated. the result showed that unlike previous findings, o. cytisi was able to degrade mtbe through direct metabolism when mtbe was present as the only carbon source. the degradation rate of mtbe was rapid, completed within 80 h. mtbe biodegradation by this strain was stimulated at low concentrations of zn(2+) (1-5 mg l(-1)) and m ...200717555790
a semi-quantitative gelc-ms analysis of temporal proteome expression in the emerging nosocomial pathogen ochrobactrum anthropi.the alpha-proteobacteria are capable of interaction with eukaryotic cells, with some members, such as ochrobactrum anthropi, capable of acting as human pathogens. o. anthropi has been the cause of a growing number of hospital-acquired infections; however, little is known about its growth, physiology and metabolism. we used proteomics to investigate how protein expression of this organism changes with time during growth.200717567905
an epidemic of chronic pseudophakic endophthalmitis due to ochrobactrum anthropi: clinical findings and managements of nine consecutive report an epidemic of o. anthropi pseudophakic endophthalmitis.200718085486
[ochrobactrum anthropi bacteremia developed after cholangiopancreatography].ochrobactrum anthropi (formerly achromobacter spp.) is an aerobic, motile, oxidase positive and lactose negative gram negative bacillus which is widely distributed in the environment and water sources. in recent publications, o. anthropi has an increasing importance as a nosocomial infection agent. the aim of this report was to present a case of o. anthropi bacteremia developed after endoscopic retrograde cholangiopancreatography (ercp). a 89-year old female patient presented with high fever one ...200717933261
early bacterial dependent induction of inducible nitric oxide synthase (inos) in epithelial cells upon transfer of cd45rb(high) cd4(+) t cells in a model for experimental colitis.both the role of inducible nitric oxide synthase (inos) in the development of inflammatory bowel disease (ibd) as well as the molecular details governing its mucosal induction remain unclear.200717879278
chromate-tolerant bacteria for enhanced metal uptake by eichhornia crassipes (mart.).a total of 85 chromate-resistant bacteria were isolated from the rhizosphere of water hyacinth grown in mariout lake, egypt, as well as the sediment and water of this habitat. only 4 (11%), 2 (8%), and 2 (8%) of isolates from each of the environments, respectively, were able to tolerate 200 mg cr (vi) l(-1). when these eight isolates were tested for their ability to tolerate other metals or to reduce chromate, they were shown to also be resistant to zn, mn, and pb, and to display different degre ...200718246718
enzyme-catalyzed formation of beta-peptides: beta-peptidyl aminopeptidases bapa and dmpa acting as beta-peptide-synthesizing recent studies, we discovered that the three beta-peptidyl aminopeptidases, bapa from sphingosinicella xenopeptidilytica 3-2w4, bapa from s. microcystinivorans y2, and dmpa from ochrobactrum anthropi lmg7991, possess the unique feature of cleaving n-terminal beta-amino acid residues from beta- and alpha/beta-peptides. herein, we investigated the use of the same three enzymes for the reverse reaction catalyzing the oligomerization of beta-amino acids and the synthesis of mixed peptides with n- ...200717886858
ochrobactrum anthropi bacteremia in a child with inborn error of mitochondrial fatty acid oxidation.objective: to report an incident of bacteremia caused by ochrobactrum anthropi. case presentation and intervention: the case of a female child aged 2 years and 10 months with a known history of long-chain 3-hydroxyacyl-coenzyme a dehydrogenase deficiency who developed o. anthropi bacteremia during hospital stay is presented. patient's history, clinical findings, laboratory and radiological investigations were thoroughly reviewed. the cultured organism was identified using microscan walkaway 96 s ...200717917448
[ochrobactrum intermedium anki, a nitrogen-fixing bacterium able to decolorize azobenzene].morphological and biochemical properties of the nitrogen-fixing strain ochrobactrum intermedium anki, intensely growing on media with azo compounds, and its resistance to various common xenobiotics were investigated. the kinetics of azobenzene conversion by o. intermedium anki was studied. under cometabolism conditions, up to 40 mg of azobenzene per liter of medium were decolorized within one week. it was shown that the strain possessed molybdenum-dependent nitrogenase activity, and its nitrogen ...200717929573
community structure analysis of reverse osmosis membrane biofilms and the significance of rhizobiales bacteria in biofouling.the biofilm community structure of a biofouled reverse osmosis (ro) membrane was examined using a polyphasic approach, and the dominant phylotypes retrieved were related to the order rhizobiales, a group of bacteria that is hitherto not implicated in membrane biofouling. a comparison with two other membrane biofilms using t-rflp fingerprinting also revealed the dominance of rhizobiales organisms. when pure culture ro biofilm isolates were cultivated aerobically in biolog microplates, most rhizob ...200717695921
reduction of toxic hexavalent chromium by ochrobactrum intermedium strain sdcr-5 stimulated by heavy metals.a cr(vi) resistant bacterial strain sdcr-5, identified as ochrobactrum intermedium on the basis of 16s rrna gene sequencing, was tolerant to high concentrations of cr(vi) up to 15 mg ml(-1) in acetate minimal medium. o. intermedium sdcr-5 reduced cr(vi) under a wide range of concentrations from 100 to 1500 microg ml(-1) and reduction was optimum at 37 degrees c and ph 7. it reduced 200 and 721 microg ml(-1) cr(vi) within 72 and 96 h, respectively. the rate of cr(vi) reduction increased with conc ...200716488604
degradation of aromatic compounds and degradative pathway of 4-nitrocatechol by ochrobactrum sp. b2.the potential capacity of a soil methyl parathion-degrading bacterium strain, ochrobactrum sp. b2, for degrading various aromatic compounds were investigated. the results showed b2 was capable of degrading diverse aromatic compounds, but amino-substituted benzene compounds, at a concentration up to 100 mg l(-1) in 4 days. b2 could use 4-nitrocatechol (4-nc) as a sole carbon and energy source with release of nitrite ion. the pathway for 4-nc degradation via 1,2,4-benzenetriol (bt) and hydroquinon ...200718074282
biocontrol and pgpr features in native strains isolated from saline soils of argentina.a bacterial collection of approximately one thousand native strains, isolated from saline soils of cordoba province (argentina), was established. from this collection, a screening to identify those strains showing plant growth promotion and biocontrol activities, as well as salt tolerance, was performed. eight native strains tolerant to 1 m: nacl and displaying plant growth promotion and/or biocontrol features were selected for further characterization. strains mep(2 )18, mrp(2 )26, mep(2 )11a, ...200717700983
partial protection against brucella infection in mice by immunization with nonpathogenic alphaproteobacteria.previous findings indicate that brucella antigens and those from nonpathogenic alphaproteobacteria (npap) are cross-recognized by the immune system. we hypothesized that immunization with npap would protect mice from brucella infection. mice were immunized subcutaneously with heat-killed ochrobactrum anthropi, sinorhizobium meliloti, mesorhizobium loti, agrobacterium tumefaciens, or brucella melitensis h38 (standard positive control) before intravenous challenge with brucella abortus 2308. cross ...200717715332
characterization of a simple bacterial consortium for effective treatment of wastewaters with reactive dyes and cr(vi).a microbial consortia consisting of three bacteria isolated from tanning and textile wastewaters revealed high capacity to simultaneously bioaccumulate dye and cr(vi). the identity of the bacteria were determined by 16s rrna gene analysis to be closely related to ochrobactrium sp., salmonella enterica and pseudomonas aeruginosa. dependence of initial ph values and range of concentrations of the dye reactive black b (33.2-103.1 mg l(-1)) and cr(vi) (19.9-127.6 mg l(-1)) were examined to find the ...200717217991
spontaneous bacterial peritonitis due to ochrobactrum anthropi: a case report.we report a case of spontaneous bacterial peritonitis from ochrobactrum anthropi. o. anthropi is recognized as an emerging pathogen in immunocompromised patients. in contrast to most previously described cases, the patient reported here had no indwelling catheter. to our knowledge, no case of o. anthropi spontaneous bacterial peritonitis has been reported in the medical literature until now.200717449955
ochrobactrum pseudintermedium sp. nov., a novel member of the family brucellaceae, isolated from human clinical samples.three novel gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. they shared more than 99.8 % of the 16s rrna gene nucleotide positions. the strains were related to ochrobactrum intermedium with about 97.48 % 16s rrna gene sequence similarity. in 16s rrna gene-, dnak- and rpob-based phylogenies, the strains were grouped in a lineage that was distinct from other ochrobactrum species in the family brucellaceae. fatty acid composition, polar lipids, quinone syste ...200717473249
pseudo-outbreak of ochrobactrum anthropi bacteremia related to cross-contamination from erythrocyte sedimentation tubes. 200717520561
crystal structure and functional characterization of a d-stereospecific amino acid amidase from ochrobactrum anthropi sv3, a new member of the penicillin-recognizing proteins.d-amino acid amidase (daa) from ochrobactrum anthropi sv3, which catalyzes the stereospecific hydrolysis of d-amino acid amides to yield the d-amino acid and ammonia, has attracted increasing attention as a catalyst for the stereospecific production of d-amino acids. in order to clarify the structure-function relationships of daa, the crystal structures of native daa, and of the d-phenylalanine/daa complex, were determined at 2.1 and at 2.4 a resolution, respectively. both crystals contain six s ...200717331533
the characteristics of rhizosphere microbes associated with plants in arsenic-contaminated soils from cattle dip sites.soil microorganisms and plants were studied in samples of arsenic-contaminated soil from two cattle dip sites. the aim was to delineate the parameters that will determine the feasibility of future remediation by growing arsenic-accumulating plants, including the identity and characteristics of some rhizosphere soil microbes. the soil samples contained high total, but low soluble arsenic concentrations which, together with other properties, resembled the previously reported characteristics of dip ...200717407787
brucellosis of the common vole (microtus arvalis).a systemic disease occurred in a wild population of the common vole microtus arvalis in south moravia (czech republic) during the years 1999-2003. acute infections were characterized by edema of extremities, occasionally with colliquating abscesses, arthritis, lymphadenitis, perforations of the skin resulting from colliquated abscesses, orchitis, and peritoneal granulomas. from the clinical samples, small gram-negative coccobacilli were isolated and identified as ochrobactrum intermedium by api ...200718021023
[microbial diversity in scorpion intestine (buthus martensii karsch)].scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ...200718062268
role of ser11 in the stabilization of the structure of ochrobactrum anthropi glutathione transferase.gsts (glutathione transferases) are a multifunctional group of enzymes, widely distributed and involved in cellular detoxification processes. in the xenobiotic-degrading bacterium ochrobactrum anthropi, gst is overexpressed in the presence of toxic concentrations of aromatic compounds such as 4-chlorophenol and atrazine. we have determined the crystal structure of the gst from o. anthropi (oagst) in complex with gsh. like other bacterial gsts, oagst belongs to the beta class and shows a similar ...200717223798
[study on nitrate-nitrogen removal and community analysis of denitrifying bacteria in downflow fixed-bed bioreactor].to study the community of denitrifying bacteria and nitrate-nitrogen removal in downflow fixed-bed bioreactor.200717424841
new enzymatic method of chiral amino acid synthesis by dynamic kinetic resolution of amino acid amides: use of stereoselective amino acid amidases in the presence of alpha-amino-epsilon-caprolactam racemase.d- and l-amino acids were produced from l- and d-amino acid amides by d-aminopeptidase from ochrobactrum anthropi c1-38 and l-amino acid amidase from pseudomonas azotoformans iam 1603, respectively, in the presence of alpha-amino-epsilon-caprolactam racemase from achromobacter obae as the catalyst by dynamic kinetic resolution of amino acid amides.200717586677
ochrobactrum haematophilum sp. nov. and ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens.three gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. on the basis of 16s rrna gene sequence similarities, these strains (ccug 30717t, ccug 43892 and ccug 38531t) were shown to belong to the alphaproteobacteria, most closely related to ochrobactrum grignonense (99.0 and 98.2% similarity to the type strain). chemotaxonomic data (major ubiquinone q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipi ...200717978211
difficulty in the identification and differentiation of clinically relevant ochrobactrum species. 200717965364
deduced catalytic mechanism of d-amino acid amidase from ochrobactrum anthropi sv3.d-amino acid amidase (daa) from ochrobactrum anthropi sv3 catalyzes d-stereospecific hydrolysis of amino acid amides. daa has attracted attention as a catalyst for the stereospecific production of d-amino acids, although the mechanism that drives the reaction has not been clear. previously, the structure of daa was classified into two types, a substrate-bound state with an ordered omega loop, and a ground state with a disordered omega loop. because the binding of the substrate facilitates orderi ...200818421151
isolation of the exoelectrogenic bacterium ochrobactrum anthropi yz-1 by using a u-tube microbial fuel cell.exoelectrogenic bacteria have potential for many different biotechnology applications due to their ability to transfer electrons outside the cell to insoluble electron acceptors, such as metal oxides or the anodes of microbial fuel cells (mfcs). very few exoelectrogens have been directly isolated from mfcs, and all of these organisms have been obtained by techniques that potentially restrict the diversity of exoelectrogenic bacteria. a special u-tube-shaped mfc was therefore developed to enrich ...200818359834
[genotypic and phenotypic similarity between bacteria of brucella genus and rhizobiaceae family as a basis for reconsideration of brucella taxonomy].accumulated data on assessment of genome of bacteria from brucella genus and rhizobiaceae family (results of sequencing, dna-rrna hybridization, 16s rrna gene sequencing etc.) as well as their phenotypic characteristics (first of all, composition of cell fatty acids) were summarized. data point to phylogenetic proximity of these bacteria and possibility to unite them in one rhizobiaceae family together with the closest relatives of brucella--first of all, with bacteria from ochrobactrum genus). ...200818368759
chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor.using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ...200818392868
novel 3-dimensional bioelectrode for mediatorless bioelectrochemical denitrification.a novel bioelectrochemical method for denitrification was developed using electricity as the electron donor. the novel electrode contained both ochrobactrum anthropi sy509, which was permeabilized as a biocatalyst, and copper powder as a conducting material. using this electrode, a high denitrification efficiency of 1 mmol n-no(3)(-)/g dry cell x h was achieved via direct electron transfer without using mediator.200818414792
ochrobactrum rhizosphaerae sp. nov. and ochrobactrum thiophenivorans sp. nov., isolated from the environment.two gram-negative, rod-shaped, non-spore-forming bacteria, pr17(t) and dsm 7216(t), isolated from the potato rhizosphere and an industrial environment, respectively, were studied for their taxonomic allocation. by rrs (16s rrna) gene sequencing, these strains were shown to belong to the alphaproteobacteria, most closely related to ochrobactrum pseudogrignonense (98.4 and 99.3 % similarity to the type strain, respectively). chemotaxonomic data (major ubiquinone q-10; major polyamines spermidine, ...200818523190
reporter genes for real-time in vive monitoring of ochrobactrum anthropi infection.despite the increasing interest in ochrobactrum anthropi as an emerging nosocomial pathogen resistant to most commonly used antimicrobials, relatively little is known about the pathogenesis and factors contributing to its virulence. also, many aspects of interaction between ochrobactrum spp. and their hosts remain unclear. the ability to monitor o. anthropi infection in the host will facilitate our understanding of the pathogenic mechanisms and will lead to better choices of antimicrobial or add ...200818637041
sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain ochrobactrum tritici scii24t.arsenic (as) is a natural metalloid, widely used in anthropogenic activities, that can exist in different oxidation states. throughout the world, there are several environments contaminated with high amounts of arsenic where many organisms can survive. the most stable arsenical species are arsenate and arsenite that can be subject to chemically and microbiologically oxidation, reduction and methylation reactions. organisms surviving in arsenic contaminated environments can have a diversity of me ...200818554386
functional assembly of a microbial consortium with autofluorescent and mineralizing activity for the biodegradation of organophosphates.organophosphorus pesticides (ops) cause serious environmental problems, and bioremediation using bacterial enzymes may provide an efficient and economical method for their detoxification. green fluorescent protein (gfp) is a stable and easily detectable marker in monitoring genetically engineered microorganisms (gems) in the environment. in our research, the methyl parathion hydrolase gene (mpd) and enhanced green fluorescent protein gene (egfp) were successfully coexpressed using petduet vector ...200818693742
efficiency of the eps emulsifier produced by ochrobactrum anthropi in different hydrocarbon bioremediation assays.ochrobactrum anthropi strain ad2 was isolated from the waste water treatment plant of an oil refinery and was identified by analysis of the sequence of the gene encoding 16s rdna. this bacterium produced exopolysaccharides in glucose nutrient broth media supplemented with various hydrocarbons (n-octane, mineral light and heavy oils and crude oils). the exopolysaccharide ad2 (eps emulsifier) synthesized showed a wide range of emulsifying activity but none of them had surfactant activity. yield pr ...200818784947
effect of glutaraldehyde treatment on stability of permeabilized ochrobactrum anthropi sy509 in nitrate removal.for practical application, the stability of permeabilized ochrobactrum anthropi sy509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. as the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. however, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. thus, wholecell immobilization u ...200819047825
isolation of urease-positive ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north india.ochrobactrum intermedium is an opportunistic human pathogen found in immunocompromised individuals. we report the case of a north indian patient with non-ulcer dyspepsia whose gastric biopsy revealed the presence of o. intermedium, along with helicobacter pylori. further description of o. intermedium was performed with 16s rrna (1500 nucleotides) and reca (1065 nucleotides) gene sequencing, and the identity and phylogenetic affiliation of the isolate was confirmed by 100% nucleotide similarity w ...200818473108
performance of a pilot-scale submerged membrane bioreactor (mbr) in treating bathing wastewater.bathing wastewater was treated by a pilot-scale submerged membrane bioreactor (mbr) for more than 60 days. the results showed that the removal rates of main pollutants of wastewater such as cod(cr), las, nh(4)(+)-n and total nitrogen (tn) were above 93%, 99%, 99%, and 90%, respectively. the results of denaturing gel gradient electrophoresis (dgge) and fluorescent in situ hybridization (fish) indicated that the bacteria were stable. the abundant nitrobacteria intercepted by the membrane led to th ...200818359626
[microbial degradation of organophosphonates by soil bacteria].bacteria that can utilize glyphosate (gp) or methylphosphonic acid (mpa) as a sole phosphorus source have been isolated from soil samples polluted with organophosphonates (op). no matter which of these compounds was predominant in the native habitat of the strains, all of them utilized methylphosphonate. some of the strains isolated from gp-polluted soil could utilize both phosphorus sources. strains growing on glyphosate only were not isolated. the isolates retained high destructive activity af ...200819004352
first isolation of two colistin-resistant emerging pathogens, brevundimonas diminuta and ochrobactrum anthropi, in a woman with cystic fibrosis: a case report.cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections.200819061488
identification and characterization of sulfur-oxidizing bacteria in an artificial wetland that treats wastewater from a tannery.wastewater from tanneries contains high concentrations of organic matter, chromium, nitrogen, and sulfur compounds. in this study, an artificial wetland is is used as the tertiary treatment in a tannery in león gto., méxico. it consists of three subplots with an area of about 450 m2. two subplots were planted with typha sp. and the third with scirpus americanus. geochemical analyses along the flowpath of the wetland show that contaminants were effectively attenuated. the most probable number tec ...200819260219
ochrobactrum anthropi septic arthritis of the acromioclavicular joint in an immunocompetent 17 year old.ochrobactrum anthropi is a ubiquitous, aerobic, gram-negative bacillus of low virulence, most frequently associated with nosocomial infections and infections related to indwelling catheters in immunocompromised hosts. this article presents the first reported case of o anthropi septic arthritis, here occurring at the acromioclavicular joint of an otherwise healthy patient, and provides treatment recommendations based on our experience.200819292339
removal of methyl parathion from artificial off-gas using a bioreactor containing a constructed microbial consortium.methyl parathion (mp), a highly toxic organophosphorus pesticide, was widely used for agriculture crop protection. during the production of mp and the process of mp-containing wastewater treatment, mp can release into the atmosphere and will do great harm to adjacent communities. a consortium comprised of an engineered microorganism and a natural p-nitrophenol (pnp) degrader was assembled for complete mineralization of mp. we genetically engineered escherichia coli bl21 (de3) enabling the overex ...200818409649
biodegradation of 2,4,6-tribromophenol by ochrobactrum sp. strain tb01.a bacterium that utilizes 2,4,6-tribromophenol (2,4,6-tbp) as sole carbon and energy source was isolated from soil contaminated with brominated pollutants. this bacterium, designated strain tb01, was identified as an ochrobactrum species. the organism degraded 100 microm of 2,4,6-tbp within 36 h in a growing culture. in addition, it released 3 mol of bromine ions from 1 mol of 2,4,6-tbp during the complete degradation of 2,4,6-tbp in a resting cell assay. moreover, cells grown on 2,4,6-tbp degra ...200818460800
specific detection and differentiation of ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. by a multi-primer pcr that targets the reca gene.ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. consequently, difficulties in their identification and differentiation have been reported. in this study, a sensitive reca gene-based multi-primer single-target pcr (mp-st-pcr) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. the specificity of the assa ...200818065669
microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates.during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ...200817728184
selection and characterization of aerobic bacteria capable of degrading commercial mixtures of low-ethoxylated nonylphenols.isolation and characterization of new bacterial strains capable of degrading nonylphenol ethoxylates (npneo) with a low ethoxylation degree, which are particularly recalcitrant to biodegradation.200817850311
biodegradation and bioremediation of endosulfan contaminated soil.among the three mixed bacterial culture ae, be, and ce, developed by enrichment technique with endosulfan as sole carbon source, consortium ce was found to be the most efficient with 72% and 87% degradation of alpha-endosulfan and beta-endosulfan, respectively, in 20 days. in soil microcosm, consortium ae, be and ce degraded alpha-endosulfan by 57%, 88% and 91%, respectively, whereas beta-endosulfan was degraded by 4%, 60% and 67% after 30 days. ochrobacterum sp., arthrobacter sp., and burkholde ...200817646098
microscopic investigations of the cr(vi) uptake mechanism of living ochrobactrum anthropi.a basic understanding related to the immobilization of chromium by bacteria is essential for chromate pollutant remediation in the environment. in this work, we studied the cr(vi) uptake mechanism of living ochrobactrum anthropi and the influence of a bacterial culture medium on the cr-immobilization process. it was found that the cr-immobilization ratio of bacteria in tris-hcl buffer is higher than in lb medium. x-ray photoelectron spectroscopy (xps) and electron paramagnetic resonance (epr) an ...200818686976
environmental conditions affecting exopolysaccharide production by pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp.three different chromium-resistant microorganisms (pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp.) were tested with regard to their eps production at different ph levels, temperatures, cr(vi) concentrations, and incubation periods. the optimum ph level was 7 for p. aeruginosa and micrococcus sp., while it was 8 for ochrobactrum sp. according to the highest eps amount at 100 mg/l cr(vi) concentration. the highest production of epss by the three bacteria was obtained under different ...200818155834
daeguia caeni gen. nov., sp. nov., isolated from sludge of a textile dye works.a gram-negative, non-spore-forming, rod-shaped bacterial strain, k107(t), was isolated from sludge collected from a textile dye works in korea and its taxonomic position was investigated by means of a polyphasic analysis. strain k107(t) contained q-10 as the predominant ubiquinone. the major fatty acids (>10% of total fatty acids) were c(18:1)omega7c and c(18:1) 2-oh. the dna g+c content was 57.0 mol%. a phylogenetic analysis based on 16s rrna gene sequences showed that strain k107(t) was closel ...200818175704
novel brucella strain (bo1) associated with a prosthetic breast implant infection.we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ...200817977982
the chromate-inducible chrbacf operon from the transposable element tnotchr confers resistance to chromium(vi) and superoxide.large-scale industrial use of chromium(vi) has resulted in widespread contamination with carcinogenic chromium(vi). the abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. here we report identification of the transposon-located (tnotchr) chromate resistance genes from the highly tolerant strain ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mm. the 7,189-bp-long tnotchr of the mixed tn21/t ...200818776016
cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order rhizobiales, and proposal of cohaesibacteraceae fam. nov.two novel gram-negative bacterial strains, designated cl-gr15(t) and cl-gr35, were isolated from coastal seawater of the east coast of korea. identical 16s rrna gene sequences were found in the two strains, and it was found that the strains represented a distinct and deep evolutionary lineage of descent in the order rhizobiales, and clustered with yet-uncultured marine bacteria. this lineage could not be associated with any of 12 known families in the order rhizobiales. the most closely related ...200818175720
spinal arthropathy associated with ochrobactrum anthropi in free-ranging cane toads (chaunus [bufo] marinus) in australia.inflammatory or degenerative pathology involving the vertebral bodies and/or ventral intervertebral joints has been described in numerous species, both captive and free ranging, including mammals, birds, and snakes, although never in amphibians. we described 15 cases of a newly recognized spinal arthropathy in adult cane toads (chaunus [bufo] marinus), an invasive species in australia. grossly appreciable lesions consisted primarily of ventral proliferation of bone and cartilage that resulted in ...200818192584
transfer of antibiotic multiresistant plasmid rp4 from escherichia coli to activated sludge situ transfer of a self-transmissible, antibiotic-multiresistant plasmid rp4 from a laboratory escherichia coli strain c600 to indigenous activated sludge bacteria was investigated using filter mating. the transfer frequency of rp4 from the donor e. coli to the bacteria that was sampled from two wastewater treatment plants was 5.1x10(-2) to 7.5x10(-1) and 4.6x10(-3) to 1.3x10(-2)/potential recipient. the isolated transconjugants showed resistance to ap, km, and tc and the presence of a plasmi ...200818930008
development of an autofluorescent whole-cell biocatalyst by displaying dual functional moieties on escherichia coli cell surfaces and construction of a coculture with organophosphate-mineralizing activity .surface display of the active proteins on living cells has enormous potential in the degradation of numerous toxic compounds. here, we report the codisplay of organophosphorus hydrolase (oph) and enhanced green fluorescent protein (gfp) on the cell surface of escherichia coli by use of the truncated ice nucleation protein (inpnc) and lpp-ompa fusion systems. the surface localization of both inpnc-oph and lpp-ompa-gfp was demonstrated by western blot analysis, immunofluorescence microscopy, and a ...200818952884
genetic diversity and phylogenetic relationships of bacteria belonging to the ochrobactrum-brucella group by reca and 16s rrna gene-based comparative sequence analysis.the genetic diversity and phylogenetic interrelationships among 106 ochrobactrum strains (o. anthropi: 72, o. intermedium: 22, o. tritici: 5, o. oryzae: 2, o. grignonense: 2, o. gallinifaecis: 1, o. lupini: 2), the type strains of the eight brucella species and other closely related taxa were studied by reca and rrs gene (16s rrna) comparative sequence analysis. both markers correctly delineated the various ochrobactrum species; however, resolution at the subspecies level was considerably higher ...200818222618
ochrobactrum anthropi septicaemia.ochrobactrum anthropi is an emerging opportunist pathogen in immunocompromised patients. we report a case of septicaemia due to o. anthropi in an elderly male patient with coronary artery disease with severe left ventricular dysfunction admitted in the intensive coronary care unit. following intraaortic balloon pump (iabp) insertion, the patient developed a haematoma at the local site, which led to septicaemia. in spite of intensive treatment, the condition of the patient continued to deteriorat ...200818227608
cysteine 10 is critical for the activity of ochrobactrum anthropi glutathione transferase and its mutation to alanine causes the preferential binding of glutathione to the h-site.the role of the evolutionarily conserved residue cys10 in ochrobactrum anthropi glutathione transferase (oagst) has been examined by replacing it with an alanine. a double mutant c10a/s11a was also prepared. the effect of the replacements on the coniugating and thiotransferase activities, and on the thermal and chemical stability of the enzyme was analyzed. our data support the view that in oagst, in contrast with other beta class gsts that display significant differences in the glutathione-bind ...200818076047
structures of d-amino-acid amidase complexed with l-phenylalanine and with l-phenylalanine amide: insight into the d-stereospecificity of d-amino-acid amidase from ochrobactrum anthropi sv3.the crystal structures of d-amino-acid amidase (daa) from ochrobactrum anthropi sv3 in complex with l-phenylalanine and with l-phenylalanine amide were determined at 2.3 and 2.2 a resolution, respectively. comparison of the l-phenylalanine amide complex with the d-phenylalanine complex reveals that the d-stereospecificity of daa might be achieved as a consequence of three structural factors: (i) the hydrophobic cavity in the region in which the hydrophobic side chain of the substrate is held, (i ...200818323628
[beta-proteorhizobia and nonrhizobial species--a review].the terms alpha- and beta-rhizobia were proposed to distinguish the rhizobial alpha- and beta-proteorhizobia respectively. in this review we present recently development of rhizobial taxonomy about beta-rhizobia in the genus burkholeria, cupriavidus isolated from mimosa and alpha-proteorhizobia belonging to methylobacterium, devosia, ochrobactrum, phyllobacterium and blastobacter lineage. furthermore, we also introduced the evolution of symbiosis related genes such as noda and nifh, and posed so ...200819160827
enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer with manipulated variables and its properties.cupriavidus sp. usmaa1020, a local isolate was able to biosynthesis poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [p(3hb-co-4hb)] copolymer with various 4hb precursors as the sole carbon source. manipulation of the culture conditions such as cell concentration, phosphate ratio and culture aeration significantly affected the synthesis of p(3hb-co-4hb) copolymer and 4hb composition. p(3hb-co-4hb) copolymer with 4hb compositions ranging from 23 to 75 mol% 4hb with various mechanical and thermal prop ...200919189144
isolation of brucella microti from mandibular lymph nodes of red foxes, vulpes vulpes, in lower austria.from the mandibular lymph nodes of wild red foxes (vulpes vulpes) hunted in the region of gmünd, lower austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. cells were fast growing, nonmotile, and agglutinated with monospecific anti-brucella (m) serum. both strains were biochemically identified as ochrobactrum anthropi by using the api 20ne test. however, sequencing of the 16s rrna and reca genes clearly identified strains 257 ...200918973444
role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons.polycyclic aromatic hydrocarbons are ubiquitous pollutants in the environment, and most high molecular weight pahs cause mutagenic, teratogenic and potentially carcinogenic effects. while several strains have been identified that degrade pahs, the present study is focused on the degradation of pahs in a marine environment by a moderately halophilic bacterial consortium. the bacterial consortium was isolated from a mixture of marine water samples collected from seven different sites in chennai, i ...200918995870
identification and antimicrobial susceptibilities of ochrobactrum spp.ochrobactrum (o.) anthropi is an opportunistic emerging pathogen closely related to the genus brucella. identification and differentiation from brucellae and other ochrobactrum spp. using routine biochemical test systems is not reliable due to the high phenotypic similarity. in this study, antibiotic susceptibilities of 103 ochrobactrum isolates were determined using etest for 19 clinically relevant antimicrobial agents. ochrobactrum strains were highly resistant to beta-lactam antibiotics, susc ...200918755630
analysis of ten brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle.the facultative intracellular bacterial pathogen brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis. currently, there are nine recognized brucella species based on host preferences and phenotypic differences. the availability of 10 different genomes consisting of two chromosomes and representing six of the species allowed for a detailed comparison among themselves and relatives in the order rhizobiales. phylogenomic analysis of ortholog families show ...200919346311
analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by dgge.a microbial consortium was obtained by enrichment culture of sea water samples collected from botan oil port in xiamen, china, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial composition and community dynamic changes based on pcr amplification of 16s rrna genes during batch culture enrichment. using the spray-plate method, three bacteria, designated a ...200919409577
electrochemical activation of nitrate reduction to nitrogen by ochrobactrum sp. g3-1 using a noncompartmented electrochemical bioreactor.a denitrification bacterium was isolated from riverbed soil and identified as ochrobactrum sp., whose specific enzymes for denitrification metabolism were biochemically assayed or confirmed with specific coding genes. the denitrification activity of strain g3-1 was proportional to glucose/nitrate balance, which was consistent with the theoretical balance (0.5). the modified graphite felt cathode with neutral red, which functions as a solid electron mediator, enhanced the electron transfer from e ...200919734723
[biodegradation of nicotine in tobacco extracts for making reconstituted tobacco by strain dn2].the purpose of the study is to use o. intermedium dn2 to degrade nicotine in tobacco extracts for making reconstituted tobacco. firstly, we studied the effects of various factors on degradation of nicotine in the extracts by strain dn2. when we added 0.1% yeast extract into the extracts, adjusted its ph value to 7.0 by ammonia solution, inoculated 15% cultures and maintained fermentation temperature of 30 degrees c, the degradation rate of nicotine by strain dn2 was the fastest. furthmore, under ...200919777819
isolation of ochrobactrum sp.qz2 from sulfide and nitrite treatment system.a bacterial strain qz2 was isolated from sludge of anoxic sulfide-oxidizing (aso) reactor. based on 16s rdna sequence analysis and morphology, the isolate was identified as ochrobactrum sp. qz2. the strain was facultative chemolithotroph, able of using sulfide to reduce nitrite anaerobically. it produced either elemental sulfur or sulfate as the product of sulfide oxidation, depending on the initial sulfide and nitrite concentrations. the optimum growth ph and temperature for ochrobactrum sp. qz ...200919010594
a conserved hydrogen-bond network stabilizes the structure of beta class glutathione s-transferases.we identified a network of hydrogen bonds that is conserved in the structures of bacterial beta class glutathione s-transferases (gsts). it is formed by three residues: a serine, a histidine and a glutamate, together with a water molecule that links the serine with the histidine. this network connects the first helix of the n-terminal glutaredoxin-like domain with the last helix of the c-terminal gst-specific all helical domain. here we show that substitution of ochrobactrum anthropi gst his15 a ...200919289099
[cellulose-utilising bacterial association]. 200919449742
development of a pcr assay for typing and subtyping of brucella the course of this study, examinations were carried out to develop a pcr-based test which allows discrimination of brucella species and biovars not targeted by the currently established gel-based pcr assays. appropriate primers were designed based on specific deletions and insertions in the different brucella genomes as determined by rapd-pcr and whole-genome comparisons. after testing the specificity of the primers with a set of 22 brucella reference strains of all species and biovars, they ...200919560966
isolation of marine benzo[a]pyrene-degrading ochrobactrum sp. bap5 and proteins characterization.a bacterial strain bap5 with a relatively high degradation ability of benzo[a]pyrene (bap) was isolated from marine sediments of xiamen western sea, china and identified as ochrobactrum sp. according to 16s rrna gene sequence as well as biolog microbial identification system. strain bap5 could grow in mineral salt medium with 50 mg/l of bap and degrade about 20% bap after 30 d of incubation. ochrobactrum sp. bap5 was able to utilize other polycyclic aromatic hydrocarbons (pahs) (such as phenanth ...200920000001
multidrug resistant gram-negative bacilli as predominant bacteremic pathogens in liver transplant recipients.bacteremias, which are often caused by gram-negative bacteria, are the most frequently occurring infectious complications after liver transplantation (lt). the aim of this study was to investigate bacteremic incidence, pathogenic spectrum, risk factors for bacteremia due to multidrug resistant (mdr) gram-negative bacilli, and its impact on mortality after lt.200919638006
multilocus sequence typing supports the hypothesis that ochrobactrum anthropi displays a human-associated subpopulation.ochrobactrum anthropi is a versatile bacterial species with strains living in very diverse habitats. it is increasingly recognized as opportunistic pathogen in hospitalized patients. the population biology of the species particularly with regard to the characteristics of the human isolates is being investigated. to address this issue, we proposed a polyphasic approach consisting in multi-locus sequence typing (mlst), multi-locus phylogeny, genomic-based fingerprinting by pulsed-field gel electro ...200920021660
[sorption and microbial degradation of glyphosphate in soil suspensions].sorption and microbial destruction of glyphosphate, the active agent of the herbicide groundbio, in suspensions of sod-podzolic and gray forest soils has been studied. according to the values of the adsorptive capacity (3560 and 8200 mg/kg, respectively) and the freundlich constants (kf, 15.6 and 18.7, respectively), these soils had a relatively high sorption capacity as related to the herbicide. sorbed glyphosphate is represented by extractable and bound (inextractable) fractions. after long-te ...200920067150
identification and characterization of the chromium (vi) responding protein from a newly isolated ochrobactrum anthropi cts-325.a gram-negative, chromium(vi) tolerant and reductive strain cts-325, isolated from a chinese chromate plant, was identified as ochrobactrum anthropi based on its biochemical properties and 16s rdna sequence analysis. it was able to tolerate up to 10 mmol/l cr(vi) and completely reduce 1 mmol/l cr(vi) to cr(iii) within 48 h. when the strain cts-325 was induced with cr(vi), a protein increased significantly in the whole cell proteins. liquid chromatography tandem mass spectrometry (lc-ms/ms) analy ...200920131597
kinetic resolution of aliphatic beta-amino acid amides by beta-aminopeptidases.the growing demand for enantiomerically pure beta-amino acids to be used in the pharmaceutical industry and as fine chemicals requires the development of new strategies for their synthesis. the beta-aminopeptidases bapa from sphingosinicella xenopeptidilytica 3-2w4, bapa from sphingosinicella microcystinivorans y2, and dmpa from ochrobactrum anthropi lmg7991 are hydrolases that possess the unique ability of cleaving n-terminal beta-amino acids from peptides and amides. hydrolysis of racemic beta ...200919449346
isolation and characterization of a cr(vi)-reduction ochrobactrum sp. strain cscr-3 from chromium landfill.a strain cscr-3 with high cr(vi)-reducing ability under alkaline conditions was isolated from a chromium landfill and identified as ochrobactrum sp. on the basis of 16s rrna gene sequence analysis. the cells were rod shaped, gram-negative and motile. the physiological characteristics and cr(vi)-reduction of the strain were also studied. the results showed that the ochrobactrum sp. strain cscr-3 was tolerant to very high concentration of cr(vi) (800 mg/l) and capable of reducing different forms o ...200918722054
[catheter- associated bacteremia caused by ochrobactrum anthropi].ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as achromobacter sp or cdc group vd. it has been isolated from the environment and from infections in usually immunocompromised human beings. the documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. here it is presente ...200920053608
bradyrhizobium spp. and sinorhizobium fredii are predominant in root nodules of vigna angularis, a native legume crop in the subtropical region of china.adzuki bean (vigna angularis) is an important legume crop native to china, but its rhizobia have not been well characterized. in the present study, a total of 60 rhizobial strains isolated from eight provinces of china were analyzed with amplified 16s rrna gene rflp, igs-rflp, and sequencing analyses of 16s rrna, atpd, reca, and nodc genes. these strains were identified as genomic species within rhizobium, sinorhizobium, mesorhizobium, bradyrhizobium, and ochrobactrum. the most abundant groups w ...200919557346
ochrobactrum anthropi peritonitis in an immunocompetent capd patient. 200919910572
role of a serine-type d-alanyl-d-alanine carboxypeptidase on the survival of ochrobactrum sp. 11a under ionic and hyperosmotic stress.the plant growth-promoting rhizobacterium, ochrobactrum sp. 11a displays a high intrinsic salinity tolerance and has been used in this work to study the molecular basis of bacterial responses to high concentrations of nacl. a collection of ochrobactrum sp. 11a mutants was generated by tn5-b21 mutagenesis and screened for sensitivity to salinity. one clone, designated pbp and unable to grow on glutamate mannitol salt agar medium supplemented with 300 mm nacl was selected and further characterized ...200919646181
zhangella mobilis gen. nov., sp. nov., a new member of the family hyphomicrobiaceae isolated from coastal seawater.a gram-negative, pale-yellow, non-spore-forming bacterium, motile with single polar flagella, strain e6t, was isolated from coastal seawater collected from tianjin, china, and its taxonomic position was investigated using a polyphasic approach. strain e6t requires nacl for growth and grows optimally at ph 8.2 and 36 degrees c and in the presence of 2.0% (w/v) nacl. it is positive for catalase and oxidase, and reduces nitrate to nitrite. the major fatty acids (>10%) are c18:1omega7c (64.71%) and ...200919620375
whole-genome-based phylogeny and divergence of the genus brucella.brucellae are worldwide bacterial pathogens of livestock and wildlife, but phylogenetic reconstructions have been challenging due to limited genetic diversity. we assessed the taxonomic and evolutionary relationships of five brucella species-brucella abortus, b. melitensis, b. suis, b. canis, and b. ovis-using whole-genome comparisons. we developed a phylogeny using single nucleotide polymorphisms (snps) from 13 genomes and rooted the tree using the closely related soil bacterium and opportunist ...200919201792
isolation and identification of dibutyl phthalate-degrading bacteria from hydrospheres in tokyo.dibutyl phthalate (dbp) is used widely as a plasticizer and is thought to negatively affect various organisms. to isolate and investigate dbp-degrading bacteria from hydrospheres in tokyo, strains were selected on ynb medium containing dbp as the sole carbon source, and candidate strains were identified by zones of clearing around the colonies. degradation of dbp by the strains was subsequently measured with hplc, and bacterial identification was accomplished using 16s rdna sequences. nineteen s ...200919700919
brucella microti: the genome sequence of an emerging pathogen.using a combination of pyrosequencing and conventional sanger sequencing, the complete genome sequence of the recently described novel brucella species, brucella microti, was determined. b. microti is a member of the genus brucella within the alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. in contrast to all other brucella species, b. microti is a fast growing and biochemically very active microorganism with a phenotype more simila ...200919653890
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