| selection and characterization of aerobic bacteria capable of degrading commercial mixtures of low-ethoxylated nonylphenols. | isolation and characterization of new bacterial strains capable of degrading nonylphenol ethoxylates (npneo) with a low ethoxylation degree, which are particularly recalcitrant to biodegradation. | 2008 | 17850311 |
| early bacterial dependent induction of inducible nitric oxide synthase (inos) in epithelial cells upon transfer of cd45rb(high) cd4(+) t cells in a model for experimental colitis. | both the role of inducible nitric oxide synthase (inos) in the development of inflammatory bowel disease (ibd) as well as the molecular details governing its mucosal induction remain unclear. | 2007 | 17879278 |
| pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus ochrobactrum. | the alpha-proteobacterial genus ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. the genomes of nine strains of ochrobactrum anthropi and eight strains of ochrobactrum intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of i-ceui dige ... | 2005 | 15995979 |
| molecular and phenotypic features for identification of the opportunistic pathogens ochrobactrum spp. | among the six species characterized within the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium are currently reported as opportunistic pathogens in humans. since the species identification is mainly based on 16s rdna analysis, the aim of this study was to search for other characteristics useful for ochrobactrum species discrimination. ribotyping, morphological and biochemical analyses, and antimicrobial susceptibility testing were performed for a panel of 35 clinical isola ... | 2005 | 16157548 |
| [ochrobactrum intermedium anki, a nitrogen-fixing bacterium able to decolorize azobenzene]. | morphological and biochemical properties of the nitrogen-fixing strain ochrobactrum intermedium anki, intensely growing on media with azo compounds, and its resistance to various common xenobiotics were investigated. the kinetics of azobenzene conversion by o. intermedium anki was studied. under cometabolism conditions, up to 40 mg of azobenzene per liter of medium were decolorized within one week. it was shown that the strain possessed molybdenum-dependent nitrogenase activity, and its nitrogen ... | 2007 | 17929573 |
| [ochrobactrum anthropi bacteremia developed after cholangiopancreatography]. | ochrobactrum anthropi (formerly achromobacter spp.) is an aerobic, motile, oxidase positive and lactose negative gram negative bacillus which is widely distributed in the environment and water sources. in recent publications, o. anthropi has an increasing importance as a nosocomial infection agent. the aim of this report was to present a case of o. anthropi bacteremia developed after endoscopic retrograde cholangiopancreatography (ercp). a 89-year old female patient presented with high fever one ... | 2007 | 17933261 |
| difficulty in the identification and differentiation of clinically relevant ochrobactrum species. | | 2007 | 17965364 |
| novel brucella strain (bo1) associated with a prosthetic breast implant infection. | we report the microbiological, biochemical, and molecular characterization of an unusual brucella strain (bo1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on dna-dna reassociation and the presence of multiple copies of is711 element suggested that the isolate was a brucella-like ... | 2008 | 17977982 |
| complete mineralisation of dimethylformamide by ochrobactrum sp. dgvk1 isolated from the soil samples collected from the coalmine leftovers. | a bacterial strain dgvk1 capable of using n,n-dimethylformamide (dmf) as sole source of carbon and nitrogen was isolated from the soil samples collected from the coalmine leftovers. the molecular phylogram generated using the complete sequence of 16s rdna of the strain dgvk1 showed close links to the bacteria grouped under brucellaceae family that belongs to alphaproteobacteria class. specifically, the 16s rdna sequence of strain dgvk1 has shown 97% similarity to ochrobactrum anthropi lmg 3331 ( ... | 2006 | 16211382 |
| brucellosis of the common vole (microtus arvalis). | a systemic disease occurred in a wild population of the common vole microtus arvalis in south moravia (czech republic) during the years 1999-2003. acute infections were characterized by edema of extremities, occasionally with colliquating abscesses, arthritis, lymphadenitis, perforations of the skin resulting from colliquated abscesses, orchitis, and peritoneal granulomas. from the clinical samples, small gram-negative coccobacilli were isolated and identified as ochrobactrum intermedium by api ... | 2007 | 18021023 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| specific detection and differentiation of ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. by a multi-primer pcr that targets the reca gene. | ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. consequently, difficulties in their identification and differentiation have been reported. in this study, a sensitive reca gene-based multi-primer single-target pcr (mp-st-pcr) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. the specificity of the assa ... | 2008 | 18065669 |
| degradation of aromatic compounds and degradative pathway of 4-nitrocatechol by ochrobactrum sp. b2. | the potential capacity of a soil methyl parathion-degrading bacterium strain, ochrobactrum sp. b2, for degrading various aromatic compounds were investigated. the results showed b2 was capable of degrading diverse aromatic compounds, but amino-substituted benzene compounds, at a concentration up to 100 mg l(-1) in 4 days. b2 could use 4-nitrocatechol (4-nc) as a sole carbon and energy source with release of nitrite ion. the pathway for 4-nc degradation via 1,2,4-benzenetriol (bt) and hydroquinon ... | 2007 | 18074282 |
| isolation and identification of a novel strain of the genus ochrobactrum with phenol-degrading activity. | a bacterial strain as1 belonging to the genus ochrobactrum, was isolated from an enriched phenol-activated sludge in egypt. this strain grew aerobically on phenol as the sole carbon source using the meta-cleavage pathway at high phenol-degrading rates compared with those in a previous report. | 2003 | 16233528 |
| comparative sequence analysis of the internal transcribed spacer 1 of ochrobactrum species. | the internal 16s/23s rdna (rrs/rrl) internal spacer region 1 (its1) of 54 ochrobactrum strains and close relatives was analysed. separation of its1 containing pcr products by gel-electrophoresis, dgge, cloning and sequencing revealed its1 length and sequence heterogeneity. we found up to 5 different allelic its1 stretches within a single strain (ochrobactrum intermedium lmg 3301t), and 2-3 different its1 alleles in o. tritici. within its1, its1c, being part of the conserved double-stranded rrn p ... | 2006 | 16352412 |
| environmental conditions affecting exopolysaccharide production by pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp. | three different chromium-resistant microorganisms (pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp.) were tested with regard to their eps production at different ph levels, temperatures, cr(vi) concentrations, and incubation periods. the optimum ph level was 7 for p. aeruginosa and micrococcus sp., while it was 8 for ochrobactrum sp. according to the highest eps amount at 100 mg/l cr(vi) concentration. the highest production of epss by the three bacteria was obtained under different ... | 2008 | 18155834 |
| daeguia caeni gen. nov., sp. nov., isolated from sludge of a textile dye works. | a gram-negative, non-spore-forming, rod-shaped bacterial strain, k107(t), was isolated from sludge collected from a textile dye works in korea and its taxonomic position was investigated by means of a polyphasic analysis. strain k107(t) contained q-10 as the predominant ubiquinone. the major fatty acids (>10% of total fatty acids) were c(18:1)omega7c and c(18:1) 2-oh. the dna g+c content was 57.0 mol%. a phylogenetic analysis based on 16s rrna gene sequences showed that strain k107(t) was closel ... | 2008 | 18175704 |
| the effects of salinity and other factors on nitrite reduction by ochrobactrum anthropi 49187. | the nitrite reductase (nir) gene was cloned from ochrobactrum anthropi 49187 and found to contain an open reading frame of 1131 nucleotides, encoding a polypeptide of 376 amino acids. the o. anthropi nir gene encodes a copper-type dissimilatory reductase based on sequence homology with other genes. the polypeptide product is predicted to form a trimeric holoenzyme of 37 kda subunits based on molecular weight estimates of extracts in activity gels. expression of the enzyme is up-regulated by nitr ... | 2006 | 16463313 |
| beneficial role of hydrophytes in removing cr(vi) from wastewater in association with chromate-reducing bacterial strains ochrobactrum intermedium and brevibacterium. | this study deals with the use of three chromium-resistant bacterial strains (ochrobactrum intermedium crt-1, brevibacterium crt-13, and crm-1) in conjunction with eichornia crassipes for the removal of toxic chromium from wastewater. bacterial strains resulted in reduced uptake of chromate into inoculated plants as compared to noninoculated control plants. in the presence of different heavy metals, chromium uptake into the plants was 28.7 and 7.15% less at an initial k2cro4 concentration of 100 ... | 2005 | 16463540 |
| genetic diversity and phylogenetic relationships of bacteria belonging to the ochrobactrum-brucella group by reca and 16s rrna gene-based comparative sequence analysis. | the genetic diversity and phylogenetic interrelationships among 106 ochrobactrum strains (o. anthropi: 72, o. intermedium: 22, o. tritici: 5, o. oryzae: 2, o. grignonense: 2, o. gallinifaecis: 1, o. lupini: 2), the type strains of the eight brucella species and other closely related taxa were studied by reca and rrs gene (16s rrna) comparative sequence analysis. both markers correctly delineated the various ochrobactrum species; however, resolution at the subspecies level was considerably higher ... | 2008 | 18222618 |
| reduction of toxic hexavalent chromium by ochrobactrum intermedium strain sdcr-5 stimulated by heavy metals. | a cr(vi) resistant bacterial strain sdcr-5, identified as ochrobactrum intermedium on the basis of 16s rrna gene sequencing, was tolerant to high concentrations of cr(vi) up to 15 mg ml(-1) in acetate minimal medium. o. intermedium sdcr-5 reduced cr(vi) under a wide range of concentrations from 100 to 1500 microg ml(-1) and reduction was optimum at 37 degrees c and ph 7. it reduced 200 and 721 microg ml(-1) cr(vi) within 72 and 96 h, respectively. the rate of cr(vi) reduction increased with conc ... | 2007 | 16488604 |
| herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops. | acetohydroxyacid synthase (ahas) inhibitors interfere with branched-chain amino acid biosynthesis by inhibiting ahas. glyphosate affects aromatic amino acid biosynthesis by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (epsps). glufosinate inhibits glutamine synthetase and blocks biosynthesis of glutamine. ahas gene variants that confer tolerance to ahas inhibitors have been discovered in plants through selection or mutagenesis. imidazolinone-tolerant crops have been commercialized base ... | 2006 | 16547651 |
| growth stimulatory effect of ochrobactrum intermedium and bacillus cereus on vigna radiata plants. | this study assessed the plant growth-promoting ability of the bacterial strains ochrobactrum intermedium (isolate crt-1) and bacillus cereus (isolate s-6). | 2006 | 16965380 |
| alkane utilization by rhodococcus strain ntu-1 alone and in its natural association with bacillus fusiformis l-1 and ochrobactrum sp. | linear (n-hexadecane) and branched (pristane) alkanes were degraded by a mixed culture isolated from an oil-contaminated field. the degradation was accompanied by formation of biofloccules. the culture was composed of rhodococcus strain ntu-1, bacillus fusiformis l-1, and ochrobactrum sp. rhodococcus strain ntu-1 carried out the degradation of the alkane via a hydroxylase. bacillus fusiformis l-1 and ochrobactrum sp. did not degrade the alkanes but aided the flocculation by forming more rigid ba ... | 2006 | 17022676 |
| multidimensional proteomic analysis of the soluble subproteome of the emerging nosocomial pathogen ochrobactrum anthropi. | we report the first large-scale gel-free proteomic analysis of the soluble subproteome of the emerging pathogen ochrobactrum anthropi. utilizing our robust offline multidimensional protein identification protocol, a total of 57 280 peptides were initially identified utilizing automated ms/ms analysis software. we describe our investigation of the heuristic protein validation tool provalt and demonstrate its ability to increase the speed and accuracy of the curation process of large-scale proteom ... | 2006 | 17081066 |
| [genotypic and phenotypic similarity between bacteria of brucella genus and rhizobiaceae family as a basis for reconsideration of brucella taxonomy]. | accumulated data on assessment of genome of bacteria from brucella genus and rhizobiaceae family (results of sequencing, dna-rrna hybridization, 16s rrna gene sequencing etc.) as well as their phenotypic characteristics (first of all, composition of cell fatty acids) were summarized. data point to phylogenetic proximity of these bacteria and possibility to unite them in one rhizobiaceae family together with the closest relatives of brucella--first of all, with bacteria from ochrobactrum genus). ... | 2008 | 18368759 |
| biodegradation kinetics of the nitramine explosive cl-20 in soil and microbial cultures. | the cyclic nitramine explosive cl-20 (c(6)h(6)n(12)o(12), 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12 -hexaazaisowurtzitane) is a relatively new energetic compound which could be a persistent organic pollutant. to follow its biodegradation dynamics, cl-20 was added to soil alone or together with organic co-substrates and n-source and incubated under oxic and anoxic conditions. without co-substrates, the cl-20 degradation was detectable only under anoxic conditions. the highest degradation rate was fou ... | 2007 | 17091356 |
| removal of methyl parathion from artificial off-gas using a bioreactor containing a constructed microbial consortium. | methyl parathion (mp), a highly toxic organophosphorus pesticide, was widely used for agriculture crop protection. during the production of mp and the process of mp-containing wastewater treatment, mp can release into the atmosphere and will do great harm to adjacent communities. a consortium comprised of an engineered microorganism and a natural p-nitrophenol (pnp) degrader was assembled for complete mineralization of mp. we genetically engineered escherichia coli bl21 (de3) enabling the overex ... | 2008 | 18409649 |
| novel 3-dimensional bioelectrode for mediatorless bioelectrochemical denitrification. | a novel bioelectrochemical method for denitrification was developed using electricity as the electron donor. the novel electrode contained both ochrobactrum anthropi sy509, which was permeabilized as a biocatalyst, and copper powder as a conducting material. using this electrode, a high denitrification efficiency of 1 mmol n-no(3)(-)/g dry cell x h was achieved via direct electron transfer without using mediator. | 2008 | 18414792 |
| deduced catalytic mechanism of d-amino acid amidase from ochrobactrum anthropi sv3. | d-amino acid amidase (daa) from ochrobactrum anthropi sv3 catalyzes d-stereospecific hydrolysis of amino acid amides. daa has attracted attention as a catalyst for the stereospecific production of d-amino acids, although the mechanism that drives the reaction has not been clear. previously, the structure of daa was classified into two types, a substrate-bound state with an ordered omega loop, and a ground state with a disordered omega loop. because the binding of the substrate facilitates orderi ... | 2008 | 18421151 |
| biodegradation of 2,4,6-tribromophenol by ochrobactrum sp. strain tb01. | a bacterium that utilizes 2,4,6-tribromophenol (2,4,6-tbp) as sole carbon and energy source was isolated from soil contaminated with brominated pollutants. this bacterium, designated strain tb01, was identified as an ochrobactrum species. the organism degraded 100 microm of 2,4,6-tbp within 36 h in a growing culture. in addition, it released 3 mol of bromine ions from 1 mol of 2,4,6-tbp during the complete degradation of 2,4,6-tbp in a resting cell assay. moreover, cells grown on 2,4,6-tbp degra ... | 2008 | 18460800 |
| isolation of urease-positive ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north india. | ochrobactrum intermedium is an opportunistic human pathogen found in immunocompromised individuals. we report the case of a north indian patient with non-ulcer dyspepsia whose gastric biopsy revealed the presence of o. intermedium, along with helicobacter pylori. further description of o. intermedium was performed with 16s rrna (1500 nucleotides) and reca (1065 nucleotides) gene sequencing, and the identity and phylogenetic affiliation of the isolate was confirmed by 100% nucleotide similarity w ... | 2008 | 18473108 |
| biodegradation of nonylphenol in soil. | we investigated the effects of various factors (brij 30, brij 35, yeast extract, hydrogen peroxide and compost) on the aerobic degradation of nonylphenol (np) in soil and characterized the structure of the microbial community in that soil. residues of np were measured using gas chromatography-mass spectrometry (gc-ms) and a change of microbial communities was demonstrated using denaturing gradient gel electrophoresis (dgge). the results showed that taichung sandy clay loam had higher np degradat ... | 2007 | 17092543 |
| ochrobactrum rhizosphaerae sp. nov. and ochrobactrum thiophenivorans sp. nov., isolated from the environment. | two gram-negative, rod-shaped, non-spore-forming bacteria, pr17(t) and dsm 7216(t), isolated from the potato rhizosphere and an industrial environment, respectively, were studied for their taxonomic allocation. by rrs (16s rrna) gene sequencing, these strains were shown to belong to the alphaproteobacteria, most closely related to ochrobactrum pseudogrignonense (98.4 and 99.3 % similarity to the type strain, respectively). chemotaxonomic data (major ubiquinone q-10; major polyamines spermidine, ... | 2008 | 18523190 |
| sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain ochrobactrum tritici scii24t. | arsenic (as) is a natural metalloid, widely used in anthropogenic activities, that can exist in different oxidation states. throughout the world, there are several environments contaminated with high amounts of arsenic where many organisms can survive. the most stable arsenical species are arsenate and arsenite that can be subject to chemically and microbiologically oxidation, reduction and methylation reactions. organisms surviving in arsenic contaminated environments can have a diversity of me ... | 2008 | 18554386 |
| degradation of polycyclic aromatic hydrocarbons (pahs) by indigenous mixed and pure cultures isolated from coastal sediments. | the goal of this paper was to quantify and characterize microorganisms (bacteria) in sediment samples contaminated with polycyclic aromatic hydrocarbons (pahs: fluorene and naphthalene). the isolated organisms were evaluated for their ability to degrade pahs compounds. the results indicated that the total number of recovered heterotrophic colony forming units was higher than zone forming units produced by the pahs compounds. there was a relationship between the biomass of the bacteria recovered ... | 1997 | 18576139 |
| detection and characterization of bacteria from the potato rhizosphere degrading n-acyl-homoserine lactone. | quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium pectobacterium carotovorum subsp. carotovorum. the signal molecules involved in quorum sensing in p. carotovorum subsp. carotovorum belong to the group of n-acyl homoserine lactones (ahls). in our study, we screened bacteria isolated from the potato rhizosphere for the ability to degrade ahls produced by p. carotovorum subsp. carotovorum. six isolates able to degrade ahls were selected for ... | 2006 | 17110970 |
| characterization of a simple bacterial consortium for effective treatment of wastewaters with reactive dyes and cr(vi). | a microbial consortia consisting of three bacteria isolated from tanning and textile wastewaters revealed high capacity to simultaneously bioaccumulate dye and cr(vi). the identity of the bacteria were determined by 16s rrna gene analysis to be closely related to ochrobactrium sp., salmonella enterica and pseudomonas aeruginosa. dependence of initial ph values and range of concentrations of the dye reactive black b (33.2-103.1 mg l(-1)) and cr(vi) (19.9-127.6 mg l(-1)) were examined to find the ... | 2007 | 17217991 |
| functional assembly of a microbial consortium with autofluorescent and mineralizing activity for the biodegradation of organophosphates. | organophosphorus pesticides (ops) cause serious environmental problems, and bioremediation using bacterial enzymes may provide an efficient and economical method for their detoxification. green fluorescent protein (gfp) is a stable and easily detectable marker in monitoring genetically engineered microorganisms (gems) in the environment. in our research, the methyl parathion hydrolase gene (mpd) and enhanced green fluorescent protein gene (egfp) were successfully coexpressed using petduet vector ... | 2008 | 18693742 |
| isolation and characterization of a cr(vi)-reduction ochrobactrum sp. strain cscr-3 from chromium landfill. | a strain cscr-3 with high cr(vi)-reducing ability under alkaline conditions was isolated from a chromium landfill and identified as ochrobactrum sp. on the basis of 16s rrna gene sequence analysis. the cells were rod shaped, gram-negative and motile. the physiological characteristics and cr(vi)-reduction of the strain were also studied. the results showed that the ochrobactrum sp. strain cscr-3 was tolerant to very high concentration of cr(vi) (800 mg/l) and capable of reducing different forms o ... | 2009 | 18722054 |
| identification and antimicrobial susceptibilities of ochrobactrum spp. | ochrobactrum (o.) anthropi is an opportunistic emerging pathogen closely related to the genus brucella. identification and differentiation from brucellae and other ochrobactrum spp. using routine biochemical test systems is not reliable due to the high phenotypic similarity. in this study, antibiotic susceptibilities of 103 ochrobactrum isolates were determined using etest for 19 clinically relevant antimicrobial agents. ochrobactrum strains were highly resistant to beta-lactam antibiotics, susc ... | 2009 | 18755630 |
| the chromate-inducible chrbacf operon from the transposable element tnotchr confers resistance to chromium(vi) and superoxide. | large-scale industrial use of chromium(vi) has resulted in widespread contamination with carcinogenic chromium(vi). the abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. here we report identification of the transposon-located (tnotchr) chromate resistance genes from the highly tolerant strain ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mm. the 7,189-bp-long tnotchr of the mixed tn21/t ... | 2008 | 18776016 |
| [isolation and characterization of a sodium dodecyl benzene sulfonate degrading bacterial strain]. | a bacterial strain, designated as wzr-a, which could utilize sodium dodecyl benzene sulfonate (sdbs) as sole carbon and energy source for growth, was isolated from contaminated river. the strain was identified as ochrobactrum anthropi based on its morphological and physiological properties, and 16s rdna sequence analysis. the optimum ph and temperature for cell growth and sdbs degradation were 7.0 and 30 degrees u, respectively. the degradation rate of sdbs by strain wzr-a was higher than 80% wh ... | 2006 | 17302167 |
| crystal structure and functional characterization of a d-stereospecific amino acid amidase from ochrobactrum anthropi sv3, a new member of the penicillin-recognizing proteins. | d-amino acid amidase (daa) from ochrobactrum anthropi sv3, which catalyzes the stereospecific hydrolysis of d-amino acid amides to yield the d-amino acid and ammonia, has attracted increasing attention as a catalyst for the stereospecific production of d-amino acids. in order to clarify the structure-function relationships of daa, the crystal structures of native daa, and of the d-phenylalanine/daa complex, were determined at 2.1 and at 2.4 a resolution, respectively. both crystals contain six s ... | 2007 | 17331533 |
| transfer of antibiotic multiresistant plasmid rp4 from escherichia coli to activated sludge bacteria. | in situ transfer of a self-transmissible, antibiotic-multiresistant plasmid rp4 from a laboratory escherichia coli strain c600 to indigenous activated sludge bacteria was investigated using filter mating. the transfer frequency of rp4 from the donor e. coli to the bacteria that was sampled from two wastewater treatment plants was 5.1x10(-2) to 7.5x10(-1) and 4.6x10(-3) to 1.3x10(-2)/potential recipient. the isolated transconjugants showed resistance to ap, km, and tc and the presence of a plasmi ... | 2008 | 18930008 |
| development of an autofluorescent whole-cell biocatalyst by displaying dual functional moieties on escherichia coli cell surfaces and construction of a coculture with organophosphate-mineralizing activity . | surface display of the active proteins on living cells has enormous potential in the degradation of numerous toxic compounds. here, we report the codisplay of organophosphorus hydrolase (oph) and enhanced green fluorescent protein (gfp) on the cell surface of escherichia coli by use of the truncated ice nucleation protein (inpnc) and lpp-ompa fusion systems. the surface localization of both inpnc-oph and lpp-ompa-gfp was demonstrated by western blot analysis, immunofluorescence microscopy, and a ... | 2008 | 18952884 |
| isolation of brucella microti from mandibular lymph nodes of red foxes, vulpes vulpes, in lower austria. | from the mandibular lymph nodes of wild red foxes (vulpes vulpes) hunted in the region of gmünd, lower austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. cells were fast growing, nonmotile, and agglutinated with monospecific anti-brucella (m) serum. both strains were biochemically identified as ochrobactrum anthropi by using the api 20ne test. however, sequencing of the 16s rrna and reca genes clearly identified strains 257 ... | 2009 | 18973444 |
| biodegradation of nicotine from tobacco waste extract by ochrobactrum intermedium dn2. | ochrobactrum intermedium dn2 was used to degrade nicotine in tobacco waste extracts. the optimal temperature and ph of nicotine degradation by strain dn2 was 30-37 degrees c and 7.0, respectively. under these optimal conditions, the average degradation rate of nicotine in a 30l fed-batch culture was 140.5 mg 1(-1) h(-1). the results of this study indicate that strain dn2 may be useful for reducing the nicotine content of reconstituted tobacco. | 2007 | 17333093 |
| [microbial degradation of organophosphonates by soil bacteria]. | bacteria that can utilize glyphosate (gp) or methylphosphonic acid (mpa) as a sole phosphorus source have been isolated from soil samples polluted with organophosphonates (op). no matter which of these compounds was predominant in the native habitat of the strains, all of them utilized methylphosphonate. some of the strains isolated from gp-polluted soil could utilize both phosphorus sources. strains growing on glyphosate only were not isolated. the isolates retained high destructive activity af ... | 2008 | 19004352 |
| ochrobactrum cytisi sp. nov., isolated from nodules of cytisus scoparius in spain. | two strains named esc1(t) and esc5 were isolated from nodules of cytisus scoparius growing in a spanish soil. phylogenetic analysis of the 16s rrna gene showed that these strains belong to the genus ochrobactrum, their closest relatives being ochrobactrum anthropi and ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. despite this high similarity, the results of dna-dna hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a ... | 2007 | 17392207 |
| effect of glutaraldehyde treatment on stability of permeabilized ochrobactrum anthropi sy509 in nitrate removal. | for practical application, the stability of permeabilized ochrobactrum anthropi sy509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. as the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. however, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. thus, wholecell immobilization u ... | 2008 | 19047825 |
| first isolation of two colistin-resistant emerging pathogens, brevundimonas diminuta and ochrobactrum anthropi, in a woman with cystic fibrosis: a case report. | cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections. | 2008 | 19061488 |
| [beta-proteorhizobia and nonrhizobial species--a review]. | the terms alpha- and beta-rhizobia were proposed to distinguish the rhizobial alpha- and beta-proteorhizobia respectively. in this review we present recently development of rhizobial taxonomy about beta-rhizobia in the genus burkholeria, cupriavidus isolated from mimosa and alpha-proteorhizobia belonging to methylobacterium, devosia, ochrobactrum, phyllobacterium and blastobacter lineage. furthermore, we also introduced the evolution of symbiosis related genes such as noda and nifh, and posed so ... | 2008 | 19160827 |
| influences of metals on kinetics of methyl tert-butyl ether biodegradation by ochrobactrum cytisi. | the influence of zinc, manganese, and nickel on the degradation of mtbe (methyl tert-butyl ether), by an aerobic mtbe-degrading strain, ochrobactrum cytisi, were investigated. the result showed that unlike previous findings, o. cytisi was able to degrade mtbe through direct metabolism when mtbe was present as the only carbon source. the degradation rate of mtbe was rapid, completed within 80 h. mtbe biodegradation by this strain was stimulated at low concentrations of zn(2+) (1-5 mg l(-1)) and m ... | 2007 | 17555790 |
| whole-genome-based phylogeny and divergence of the genus brucella. | brucellae are worldwide bacterial pathogens of livestock and wildlife, but phylogenetic reconstructions have been challenging due to limited genetic diversity. we assessed the taxonomic and evolutionary relationships of five brucella species-brucella abortus, b. melitensis, b. suis, b. canis, and b. ovis-using whole-genome comparisons. we developed a phylogeny using single nucleotide polymorphisms (snps) from 13 genomes and rooted the tree using the closely related soil bacterium and opportunist ... | 2009 | 19201792 |
| identification and characterization of sulfur-oxidizing bacteria in an artificial wetland that treats wastewater from a tannery. | wastewater from tanneries contains high concentrations of organic matter, chromium, nitrogen, and sulfur compounds. in this study, an artificial wetland is is used as the tertiary treatment in a tannery in león gto., méxico. it consists of three subplots with an area of about 450 m2. two subplots were planted with typha sp. and the third with scirpus americanus. geochemical analyses along the flowpath of the wetland show that contaminants were effectively attenuated. the most probable number tec ... | 2008 | 19260219 |
| community structure analysis of reverse osmosis membrane biofilms and the significance of rhizobiales bacteria in biofouling. | the biofilm community structure of a biofouled reverse osmosis (ro) membrane was examined using a polyphasic approach, and the dominant phylotypes retrieved were related to the order rhizobiales, a group of bacteria that is hitherto not implicated in membrane biofouling. a comparison with two other membrane biofilms using t-rflp fingerprinting also revealed the dominance of rhizobiales organisms. when pure culture ro biofilm isolates were cultivated aerobically in biolog microplates, most rhizob ... | 2007 | 17695921 |
| biocontrol and pgpr features in native strains isolated from saline soils of argentina. | a bacterial collection of approximately one thousand native strains, isolated from saline soils of cordoba province (argentina), was established. from this collection, a screening to identify those strains showing plant growth promotion and biocontrol activities, as well as salt tolerance, was performed. eight native strains tolerant to 1 m: nacl and displaying plant growth promotion and/or biocontrol features were selected for further characterization. strains mep(2 )18, mrp(2 )26, mep(2 )11a, ... | 2007 | 17700983 |
| in vitro potency of doripenem tested against an international collection of rarely isolated bacterial pathogens. | doripenem, a new 1beta-methyl parenteral carbapenem, has very broad-spectrum activity against gram-positive and gram-negative aerobic bacteria. as noted here, the spectrum and potency extended to many rarely isolated species sampled by the doripenem global surveillance program. among the species or species groups with <or=0.14% prevalence (1959 strains tested), doripenem was active against 98.9% of enterobacteriaceae at <or=0.5 microg/ml. similarly, more than 90% of other rarely isolated gram-ne ... | 2009 | 19302927 |
| analysis of ten brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. | the facultative intracellular bacterial pathogen brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis. currently, there are nine recognized brucella species based on host preferences and phenotypic differences. the availability of 10 different genomes consisting of two chromosomes and representing six of the species allowed for a detailed comparison among themselves and relatives in the order rhizobiales. phylogenomic analysis of ortholog families show ... | 2009 | 19346311 |
| bacterial communities in a crude oil gathering and transferring system (china). | bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ... | 2009 | 19351561 |
| enzyme-catalyzed formation of beta-peptides: beta-peptidyl aminopeptidases bapa and dmpa acting as beta-peptide-synthesizing enzymes. | in recent studies, we discovered that the three beta-peptidyl aminopeptidases, bapa from sphingosinicella xenopeptidilytica 3-2w4, bapa from s. microcystinivorans y2, and dmpa from ochrobactrum anthropi lmg7991, possess the unique feature of cleaving n-terminal beta-amino acid residues from beta- and alpha/beta-peptides. herein, we investigated the use of the same three enzymes for the reverse reaction catalyzing the oligomerization of beta-amino acids and the synthesis of mixed peptides with n- ... | 2007 | 17886858 |
| evaluation of ochrobactrum anthropi trs-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease. | to evaluate ochrobactrum anthropi trs-2 isolated from tea rhizosphere and its talc based formulation for growth promotion and management of brown root rot disease of tea. | 2009 | 19426277 |
| ochrobactrum anthropi bacteremia in a child with inborn error of mitochondrial fatty acid oxidation. | objective: to report an incident of bacteremia caused by ochrobactrum anthropi. case presentation and intervention: the case of a female child aged 2 years and 10 months with a known history of long-chain 3-hydroxyacyl-coenzyme a dehydrogenase deficiency who developed o. anthropi bacteremia during hospital stay is presented. patient's history, clinical findings, laboratory and radiological investigations were thoroughly reviewed. the cultured organism was identified using microscan walkaway 96 s ... | 2007 | 17917448 |
| [cellulose-utilising bacterial association]. | | 2009 | 19449742 |
| identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium ochrobactrum tritici scii24. | microbial biotransformations have a major impact on environments contaminated with toxic elements, including arsenic, resulting in an increasing interest in strategies responsible for how bacteria cope with arsenic. in the present work, we investigated the metabolism of this metalloid in the bacterium ochrobactrum tritici scii24. this heterotrophic organism contains two different ars operons and is able to oxidize arsenite to arsenate. the presence of arsenite oxidase genes in this organism was ... | 2009 | 19525272 |
| the differential interaction of brucella and ochrobactrum with innate immunity reveals traits related to the evolution of stealthy pathogens. | during evolution, innate immunity has been tuned to recognize pathogen-associated molecular patterns. however, some alpha-proteobacteria are stealthy intracellular pathogens not readily detected by this system. brucella members follow this strategy and are highly virulent, but other brucellaceae like ochrobactrum are rhizosphere inhabitants and only opportunistic pathogens. to gain insight into the emergence of the stealthy strategy, we compared these two phylogenetically close but biologically ... | 2009 | 19529776 |
| novel denitrifying bacterium ochrobactrum anthropi yd50.2 tolerates high levels of reactive nitrogen oxides. | most studies of bacterial denitrification have used nitrate (no3-) as the first electron acceptor, whereas relatively less is understood about nitrite (no2-) denitrification. we isolated novel bacteria that proliferated in the presence of high levels of no2- (72 mm). strain yd50.2, among several isolates, was taxonomically positioned within the alpha subclass of proteobacteria and identified as ochrobactrum anthropi yd50.2. this strain denitrified no2-, as well as no3-. the gene clusters for den ... | 2009 | 19542343 |
| ochrobactrum haematophilum sp. nov. and ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. | three gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. on the basis of 16s rrna gene sequence similarities, these strains (ccug 30717t, ccug 43892 and ccug 38531t) were shown to belong to the alphaproteobacteria, most closely related to ochrobactrum grignonense (99.0 and 98.2% similarity to the type strain). chemotaxonomic data (major ubiquinone q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipi ... | 2007 | 17978211 |
| development of a pcr assay for typing and subtyping of brucella species. | in the course of this study, examinations were carried out to develop a pcr-based test which allows discrimination of brucella species and biovars not targeted by the currently established gel-based pcr assays. appropriate primers were designed based on specific deletions and insertions in the different brucella genomes as determined by rapd-pcr and whole-genome comparisons. after testing the specificity of the primers with a set of 22 brucella reference strains of all species and biovars, they ... | 2009 | 19560966 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| metalworking fluids biodiversity characterization. | hypersensitivity pneumonitis of machinists associated with metalworking fluids (mwf) was recently linked to mycobacterium immunogenum. in addition to mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as pseudomonas, were little studied. this report intended to quantify and characterize the microbial load of 44 in-use mwf. | 2010 | 19614850 |
| cysteine 10 is critical for the activity of ochrobactrum anthropi glutathione transferase and its mutation to alanine causes the preferential binding of glutathione to the h-site. | the role of the evolutionarily conserved residue cys10 in ochrobactrum anthropi glutathione transferase (oagst) has been examined by replacing it with an alanine. a double mutant c10a/s11a was also prepared. the effect of the replacements on the coniugating and thiotransferase activities, and on the thermal and chemical stability of the enzyme was analyzed. our data support the view that in oagst, in contrast with other beta class gsts that display significant differences in the glutathione-bind ... | 2008 | 18076047 |
| multidrug resistant gram-negative bacilli as predominant bacteremic pathogens in liver transplant recipients. | bacteremias, which are often caused by gram-negative bacteria, are the most frequently occurring infectious complications after liver transplantation (lt). the aim of this study was to investigate bacteremic incidence, pathogenic spectrum, risk factors for bacteremia due to multidrug resistant (mdr) gram-negative bacilli, and its impact on mortality after lt. | 2009 | 19638006 |
| an epidemic of chronic pseudophakic endophthalmitis due to ochrobactrum anthropi: clinical findings and managements of nine consecutive cases. | to report an epidemic of o. anthropi pseudophakic endophthalmitis. | 2007 | 18085486 |
| cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order rhizobiales, and proposal of cohaesibacteraceae fam. nov. | two novel gram-negative bacterial strains, designated cl-gr15(t) and cl-gr35, were isolated from coastal seawater of the east coast of korea. identical 16s rrna gene sequences were found in the two strains, and it was found that the strains represented a distinct and deep evolutionary lineage of descent in the order rhizobiales, and clustered with yet-uncultured marine bacteria. this lineage could not be associated with any of 12 known families in the order rhizobiales. the most closely related ... | 2008 | 18175720 |
| brucella microti: the genome sequence of an emerging pathogen. | using a combination of pyrosequencing and conventional sanger sequencing, the complete genome sequence of the recently described novel brucella species, brucella microti, was determined. b. microti is a member of the genus brucella within the alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. in contrast to all other brucella species, b. microti is a fast growing and biochemically very active microorganism with a phenotype more simila ... | 2009 | 19653890 |
| spinal arthropathy associated with ochrobactrum anthropi in free-ranging cane toads (chaunus [bufo] marinus) in australia. | inflammatory or degenerative pathology involving the vertebral bodies and/or ventral intervertebral joints has been described in numerous species, both captive and free ranging, including mammals, birds, and snakes, although never in amphibians. we described 15 cases of a newly recognized spinal arthropathy in adult cane toads (chaunus [bufo] marinus), an invasive species in australia. grossly appreciable lesions consisted primarily of ventral proliferation of bone and cartilage that resulted in ... | 2008 | 18192584 |
| paenochrobactrum gallinarii gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of pseudochrobactrum glaciei as paenochrobactrum glaciei comb. nov. | a gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (sa25(t)) was isolated from air of a duck barn. 16s rrna gene and reca sequence analyses clearly placed the isolate in the vicinity of the brucella-ochrobactrum-pseudochrobactrum group, with the closest relative being pseudochrobactrum glaciei kmm 3858(t). this allocation was confirmed by analyses of the quinone system (ubiquinone q-10), fatty acid data (major fatty acids c(18 : 1)omega7c and c(19 : 0) cyclo o ... | 2010 | 19684318 |
| ochrobactrum ciceri sp. nov., isolated from nodules of cicer arietinum. | a gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, ca-34(t), was isolated from nodules of chickpea (cicer arietinum) in pakistan and studied for its taxonomic affiliation. the almost full-length 16s rrna gene sequence showed highest similarities to those of strains of the genus ochrobactrum. based on results of maldi-tof ms and 16s rrna gene sequence similarity (98.6 %), strain ca-34(t) and ochrobactrum intermedium lmg 3301(t) are phylogenetic neighbours; the two ... | 2010 | 19684324 |
| isolation and identification of dibutyl phthalate-degrading bacteria from hydrospheres in tokyo. | dibutyl phthalate (dbp) is used widely as a plasticizer and is thought to negatively affect various organisms. to isolate and investigate dbp-degrading bacteria from hydrospheres in tokyo, strains were selected on ynb medium containing dbp as the sole carbon source, and candidate strains were identified by zones of clearing around the colonies. degradation of dbp by the strains was subsequently measured with hplc, and bacterial identification was accomplished using 16s rdna sequences. nineteen s ... | 2009 | 19700919 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |
| characterization and phylogenetic analysis of biodemulsifier-producing bacteria. | based on demulsification performance, twenty biodemulsifier-producing strains were isolated from various environmental sources. five of them achieved nearly or over 90% of emulsion breaking ratio within 24 h. with the aid of biochemical and physiological tests and 16s rdna analysis, these isolates were classified into eleven genera, in which six genera (brevibacillus sp., dietzia sp., ochrobactrum sp., pusillimonas sp., sphingopyxis sp. and achromobacter sp.) were firstly reported as demulsifyin ... | 2010 | 19720525 |
| ochrobactrum anthropi septicaemia. | ochrobactrum anthropi is an emerging opportunist pathogen in immunocompromised patients. we report a case of septicaemia due to o. anthropi in an elderly male patient with coronary artery disease with severe left ventricular dysfunction admitted in the intensive coronary care unit. following intraaortic balloon pump (iabp) insertion, the patient developed a haematoma at the local site, which led to septicaemia. in spite of intensive treatment, the condition of the patient continued to deteriorat ... | 2008 | 18227608 |
| percutaneous balloon pericardiotomy for the treatment of infected pericardial effusion with tamponade. | percutaneous balloon pericardiotomy has been used as an alternative for surgically created pericardial window mainly for the management of malignant pericardial effusions in critically ill patients. we describe a patient with purulent pericardial effusion and cardiac tamponade who was treated successfully and without complications with percutaneous balloon pericardiotomy. | 2006 | 16816447 |
| performance of a pilot-scale submerged membrane bioreactor (mbr) in treating bathing wastewater. | bathing wastewater was treated by a pilot-scale submerged membrane bioreactor (mbr) for more than 60 days. the results showed that the removal rates of main pollutants of wastewater such as cod(cr), las, nh(4)(+)-n and total nitrogen (tn) were above 93%, 99%, 99%, and 90%, respectively. the results of denaturing gel gradient electrophoresis (dgge) and fluorescent in situ hybridization (fish) indicated that the bacteria were stable. the abundant nitrobacteria intercepted by the membrane led to th ... | 2008 | 18359626 |
| isolation of the exoelectrogenic bacterium ochrobactrum anthropi yz-1 by using a u-tube microbial fuel cell. | exoelectrogenic bacteria have potential for many different biotechnology applications due to their ability to transfer electrons outside the cell to insoluble electron acceptors, such as metal oxides or the anodes of microbial fuel cells (mfcs). very few exoelectrogens have been directly isolated from mfcs, and all of these organisms have been obtained by techniques that potentially restrict the diversity of exoelectrogenic bacteria. a special u-tube-shaped mfc was therefore developed to enrich ... | 2008 | 18359834 |
| ochrobactrum anthropi peritonitis in an immunocompetent capd patient. | | 2009 | 19910572 |
| chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor. | using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ... | 2008 | 18392868 |
| microbial conversion of 5-sulfoisophthalic acid into 5-hydroxyisophthalic acid by ochrobactrum anthropi s9. | 5-hydroxyisophthalic acid-producing microorganisms were isolated from enrichment cultures using 5-sulfoisophthalic acid as a sulfur source. one bacterium, ochrobactrum anthropi s9, had the highest 5-sulfoisophthalic acid-degrading activity, and stoichiometrically formed 5-hydroxyisophthalic acid, a raw material for polymer synthesis. under optimum culture conditions, 1.3 mm 5-hydroxyisophthalic acid accumulated in the medium by 60 h. the addition of na(2)so(4), l: -methionine or l: -cysteine at ... | 2010 | 19941033 |
| chronic endophthalmitis after cataract surgery secondary to ochrobactrum anthropi. | | 2009 | 18483495 |
| multilocus sequence typing supports the hypothesis that ochrobactrum anthropi displays a human-associated subpopulation. | ochrobactrum anthropi is a versatile bacterial species with strains living in very diverse habitats. it is increasingly recognized as opportunistic pathogen in hospitalized patients. the population biology of the species particularly with regard to the characteristics of the human isolates is being investigated. to address this issue, we proposed a polyphasic approach consisting in multi-locus sequence typing (mlst), multi-locus phylogeny, genomic-based fingerprinting by pulsed-field gel electro ... | 2009 | 20021660 |
| [catheter- associated bacteremia caused by ochrobactrum anthropi]. | ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as achromobacter sp or cdc group vd. it has been isolated from the environment and from infections in usually immunocompromised human beings. the documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. here it is presente ... | 2009 | 20053608 |
| reporter genes for real-time in vive monitoring of ochrobactrum anthropi infection. | despite the increasing interest in ochrobactrum anthropi as an emerging nosocomial pathogen resistant to most commonly used antimicrobials, relatively little is known about the pathogenesis and factors contributing to its virulence. also, many aspects of interaction between ochrobactrum spp. and their hosts remain unclear. the ability to monitor o. anthropi infection in the host will facilitate our understanding of the pathogenic mechanisms and will lead to better choices of antimicrobial or add ... | 2008 | 18637041 |
| microscopic investigations of the cr(vi) uptake mechanism of living ochrobactrum anthropi. | a basic understanding related to the immobilization of chromium by bacteria is essential for chromate pollutant remediation in the environment. in this work, we studied the cr(vi) uptake mechanism of living ochrobactrum anthropi and the influence of a bacterial culture medium on the cr-immobilization process. it was found that the cr-immobilization ratio of bacteria in tris-hcl buffer is higher than in lb medium. x-ray photoelectron spectroscopy (xps) and electron paramagnetic resonance (epr) an ... | 2008 | 18686976 |
| characterization of a cd(2+)-resistant strain of ochrobactrum sp. isolated from slag disposal site of an iron and steel factory. | a cadmium-resistant bacterium designated as cdsp9 was isolated from the slag disposal site of iisco, burnpur, west bengal, india. the isolate was identified as ochrobactrum sp. on the basis of 16s rdna sequence-based molecular phylogenetic approach and phenotypic characteristics. it is a gram negative, short rod (0.5-1.0 micro), aerobic bacterium, growing well in lb medium between temperatures 10-42 degrees c, ph 6.0-9.0, and between 2 and 6% nacl. the most preferred nitrogen and carbon sources ... | 2010 | 20091408 |
| identification of an unusual brucella strain (bo2) from a lung biopsy in a 52 year-old patient with chronic destructive pneumonia. | brucellosis is primarily a zoonotic disease caused by brucella species. there are currently ten brucella spp. including the recently identified novel b. inopinata sp. isolated from a wound associated with a breast implant infection. in this study we report on the identification of an unusual brucella-like strain (bo2) isolated from a lung biopsy in a 52-year-old patient in australia with a clinical history of chronic destructive pneumonia. | 2010 | 20105296 |
| efficiency of the eps emulsifier produced by ochrobactrum anthropi in different hydrocarbon bioremediation assays. | ochrobactrum anthropi strain ad2 was isolated from the waste water treatment plant of an oil refinery and was identified by analysis of the sequence of the gene encoding 16s rdna. this bacterium produced exopolysaccharides in glucose nutrient broth media supplemented with various hydrocarbons (n-octane, mineral light and heavy oils and crude oils). the exopolysaccharide ad2 (eps emulsifier) synthesized showed a wide range of emulsifying activity but none of them had surfactant activity. yield pr ... | 2008 | 18784947 |
| ochrobactrum anthropi bacteremia in a preterm infant with meconium peritonitis. | ochrobactrum anthropi is a non-fermenting gram-negative rod that was identified as a pathogenic microorganism during the past decade. o. anthropi is extensively distributed in the environment, and has been found in hospital and environmental water sources. o. anthropi infection is rare in childhood. we report a case of o. anthropi bacteremia in a preterm infant with a peritoneal lavage catheter and meconium peritonitis. | 2009 | 18842433 |
| differences in two-component signal transduction proteins among the genus brucella: implications for host preference and pathogenesis. | two-component systems (tcss) are the predominant bacterial signal transduction mechanisms. species of the genus brucella are genetically highly related and differ mainly in mammalian host adaptation and pathogenesis. in this study, tcs proteins encoded in the available genome sequences of brucella species have been identified using bioinformatic methods. all the brucella species share an identical set of tcs proteins, and the number of tcs proteins in the closely related opportunistic human path ... | 2010 | 20153589 |