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aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis angusticollis.to demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis.200211849325
ochrobactrum anthropi bacteremia in pediatric oncology patients.ochrobactrum anthropi is an emerging pathogen in immunocompromised hosts, particularly in patients with indwelling catheters. we report the characteristics of 14 o. anthropi bacteremic episodes in 11 children with hickman-type central catheters. children presented with fever and nonspecific clinical manifestations. bacteremia was successfully treated with antibiotics, but catheter removal was necessary to achieve cure in four cases.200211791106
recombinant ochrobactrum anthropi expressing brucella abortus cu,zn superoxide dismutase protects mice against b. abortus infection only after switching of immune responses to th1 type.the members of the genus brucella are gram-negative, facultatively intracellular bacterial pathogens that cause brucellosis in many animal species and humans. although live, attenuated vaccines are available to protect several animal species from the disease, there is no safe and effective vaccine for human use. here we report that a bacterium that is closely related to brucella species, ochrobactrum anthropi, can be used as a vaccine vector for the delivery of brucella antigens to mice, leading ...200211953393
diversity of chromium-resistant and -reducing bacteria in a chromium-contaminated activated sludge.this study attempts to establish a relationship between the cr(vi) resistance of the culturable microbial community and the cr(vi) resistance and cr(vi)-reducing ability of representative strains of each population, in order to assess whether these are exclusive characteristics of one microbial group or abilities shared among many groups.200211972686
monoassociation of scid mice with helicobacter muridarum, but not four other enterics, provokes ibd upon receipt of t cells.recently, a number of animal models for different aspects of inflammatory bowel disease (ibd) have been developed. the aim of this study was to use one of these to determine whether particular, ostensibly innocuous, intestinal bacteria could provoke or exacerbate ibd.200211984521
toxicity and biodegradation of diamines.phytotoxicity and cytotoxicity of 2,4-diaminotoluene (2,4-d), 4,4'-methylenedianiline (4,4-d), and 1,6-hexanediamine (1,6-d) were investigated by observing the germination of young radish seeds and the viability of hela cells, respectively. 2,4-d showed the highest, 4,4-d intermediate, and 1,6-d lowest cytotoxicity. however, the phytotoxicity decreased in the order of 4,4-d > 2,4-d > 1,6-d. contrary to the results previously reported, in the modified sturm test the activated sludge degraded 2,4- ...200211990359
bacterial colonisation in the gut of phlebotomus duboseqi (diptera: psychodidae): transtadial passage and the role of female diet.bacteria isolated from the gut of different developmental stages of philebotomus duboseqi neveu-lcmaire, 1906 belonged almost all to aerobic or facultatively anaerobic gram-negative rods. in females, the highest bacterial counts were observed two days after bloodfeeding; seven days after bloodfeeding the bacterial counts returned to pre-feeding levels. most isolates were identified phenotypically as ochrobactrum sp. the distinctiveness and homogeneity of the phenotypic and genotypic characterist ...200211993554
d,l-hydantoinase activity of an ochrobactrum anthropi strain.a microorganism with the ability to release methionine from d,l-(2-methylthioethyl) hydantoin (strain 245) was isolated from soil. the aim of this study was the identification of the strain and the adjustment of the conditions of growth and of the enzymatic reaction, in order to achieve high specific activities of bioconversion of the hydantoin.200212010542
molecular characterization of a salt-tolerant bacterial community in the rice rhizosphere.the diversity of salt-tolerant bacteria present in the rhizosphere of oryza sativa was investigated. fourteen bacterial strains, isolated after enrichment in nitrogen-free, semi-solid medium and showing tolerance to 3% nacl, were analyzed by restriction patterns produced by amplified dna coding for 16s rdna (ardra) with enzymes sau3ai, alui and rsai which showed that they were represented by 4 ardra types. biodiversity among the 14 strains was also analyzed by the random amplified polymorphic dn ...200212455705
development and characterization of murine monoclonal antibodies specific for dissimilatoric copper nitrite reductase.several hybridoma cell lines from mice were established, producing monoclonal antibodies (mabs) directed against the dissimilatoric copper nitrite reductase (dnir) to detect actual denitrifying bacteria at the single cell level under nondestructive conditions in the environment. the mice were immunized with native or recombinant enzyme gained from two different bacteria, ochrobactrum anthropi and alcaligenes faecalis. the antibodies obtained could be divided into two groups according to their di ...200212470477
population analysis of a binary bacterial culture by multi-parametric flow cytometry.to study the degradation of a xenobiotic that requires a mixed culture it is essential to monitor the proportions and to control the population dynamics of the component strains. for these purposes fluorochromising techniques and multi-parametric flow cytometry were used to follow rhodococcus erythropolis k2-3 and ochrobactrum anthropi k2-14, both of which are needed to degrade 4-(2,4-dichlorophenoxy)butyric acid (2,4-db). although the two strains can grow in constant proportions in mixed cultur ...200212067522
the effects of nacl and some heavy metals on the denitrification activity of ochrobactrum anthropi.ochrobactrum anthropi is a well-known gram-negative bacterium, with the ability to degrade atrazine, urea-formaldehyde and chlorophenols. investigation were made of the nitrate and nitrite reduction capacities of the strain in succinate and glucose media, and the tolerance of its denitrification to nacl and some heavy metals. succinate proved to be a better carbon source to drive denitrification by o. anthropi. batch fermentation studies in anaerobic succinate medium indicated reduction capaciti ...200212210551
ochrobactrum anthropi bloodstream infection complicating hemodialysis.we report two cases of bacteremia with ochrobactrum anthropi in patients on hemodialysis. bacteremia became clinically manifest by recurrent febrile episodes during and after dialysis. in one patient, bacteremia persisted after ciprofloxacin therapy and was cleared only by removal of the dialysis catheter and a 3-week course of gentamicin. the second patient remained intermittently bacteremic for more than 3 months, although the dialysis catheter had been replaced. a medline search revealed only ...200212324938
microbial population in a hydrogen-dependent denitrification reactor.the bacterial population in an h2-dependent denitrification system was studied. the laboratory set-up was designed for the treatment of potable water and consisted of an electrochemical cell, where the water to be treated was enriched with h2 prior to entering a bioreactor. bioreactors (columns packed with granulated active carbon) were inoculated with denitrifying bacterial strains isolated from a previous reactor, then sampled immediately after inoculation, or after 1 or 3 months of continuous ...200212405417
ochrobactrum anthropi bacteraemia in immunocompetent children.ochrobactrum anthropi is an emerging pathogen in immunocompromised patients but infections with the bacterium have very rarely been documented in normal hosts. we report the characteristics of o. anthropi bacteraemia in 11 immunocompetent children, aged 2 months to 7 y, hospitalized in a general hospital during a 5-y period. children commonly presented with fever, non-specific respiratory or gastrointestinal manifestations, leukocytosis and neutrophilia and had a rapid recovery, even when they d ...200212578145
[species identification and molecular epidemiology of bacteria belonging to ochrobactrum genus].two species of medical interest belong to the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium. they are members of the microbiota of soil and an increasing number of works report the isolation of o. anthropi from clinical specimen, especially from immunocompromised patients and nosocomial infection. involving of each species in human infection is poorly estimated due to unclear differential phenotypic characters. we performed 16s rdna sequencing for identification of 20 cl ...200312628286
misidentification of brucella melitensis as ochrobactrum anthropi by api 20ne. 200312721322
development of a plating medium for selection of helicobacter pylori from water samples.the goal of this study was to develop a simple plating medium to allow large-scale screening of water samples for the presence of helicobacter pylori. five conventional plating media (brain heart infusion, brucella agar, columbia blood agar base, campylobacter agar kit skirrow, and hpspa medium), each containing a commercial antibiotic supplement, were initially evaluated. eight strains selected as common waterborne organisms (acinetobacter, aeromonas, bacillus, escherichia coli, enterobacter, e ...200312732566
palmate newt mortality at an english breeding site. 200312733567
heavy metal biosorption by biomass of ochrobactrum anthropi producing exopolysaccharide in activated sludge.the removal of chromium, cadmium and copper, toxic metals of high environmental priority due to their toxicity, from dilute aqueous solutions has been studied in the present work, applying a dead exopolysaccharide producing bacterium, ochrobactrum anthropi, isolated from activated sludge. particularly, the effect of ph, metal concentration and the effects of contact time were considered. optimum adsorption ph values of chromium(vi), cadmium(ii) and copper(ii) were 2.0, 8.0 and 3.0 respectively. ...200312835060
towards a standardized format for the description of a novel species (of an established genus): ochrobactrum gallinifaecis sp. nov.a format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. the abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: a gram-negative, rod- ...200312807218
liquid chromatography-electrospray mass spectrometry study of cysteine-10 s-glutathiolation in recombinant glutathione s-transferase of ochrobactrum anthropi.glutathione s-transferase of ochrobactrum anthropi (oagst), a bacterium isolated from soils contaminated by xenobiotic pollutants, was recently purified, cloned and characterised in our laboratories. the recombinant oagst (roagst), highly expressed in escherichia coli, when purified by glutathione-affinity chromatography and then analysed by electrospray ionisation mass spectrometry (esi-ms), has evidenced a disulphide bond with glutathione (s-glutathiolation), which was removable by reduction w ...200312650762
detection methods for the expression of the dissimilatory copper-containing nitrite reductase gene (dnirk) in environmental samples.in situ assays, based on monoclonal antibodies (mabs), were developed to study the microbial expression of the bacterial dissimilatory copper-containing nitrite reductase gene (dnirk), one of the key enzymes involved in denitrification, in different ecosystems. with a combination of an anti-dnirk mab and phylogenetic oligonucleotide probes, it is possible to bring structural and functional aspects of microbial communities together. to perform a double labelling, yielding a high signal strength f ...200314499994
characterisation of bacterial cultures enriched on the chlorophenoxyalkanoic acid herbicides 4-(2,4-dichlorophenoxy) butyric acid and 4-(4-chloro-2-methylphenoxy) butyric acid.the aim of this study was to enrich and characterise bacterial consortia from soils around a herbicide production plant through their capability to degrade the herbicides 4-(2,4-dichlorophenoxy) butyric acid (2,4-db) and 4-(4-chloro-2-methylphenoxy) butyric acid (mcpb). partial 16s rrna gene sequencing revealed members of the genera stenotrophomonas, brevundimonas, pseudomonas, and ochrobactrum in the 2,4-db- and mcpb-degrading communities. the degradation of 2,4-db and mcpb was facilitated by t ...200314513383
ochrobactrum anthropi misidentified as shewanella putrefaciens. 200312958302
analysis of bacterial dna patterns--an approach for controlling biotechnological processes.optimisation of biotechnological processes catalysed by microbial cells requires detailed information about operational limits of the single cells. their performance is correlated with distinct physiological states. we related these states to cell cycle events, which were found to proceed extremely diversely in different bacterial strains. characteristic dna patterns were found flow cytometrically, depending on the type of strain, substrates and growth conditions involved; this information can b ...200314607431
genes for an alkaline d-stereospecific endopeptidase and its homolog are located in tandem on bacillus cereus genome.alkaline d-peptidase (adp) from bacillus cereus df4-b is a d-stereospecific endopeptidase acting on oligopeptides composed of d-phenylalanine and the primary structure deduced from its gene, adp, shows a similarity with d-stereospecific hydrolases from ochrobactrum anthropi strains. we have isolated dna fragments covering the flanking region of adp from df4-b genome and found an additional gene, adp2, located upstream of adp. the deduced amino acid sequence of adp2 showed 96% and 85% identity wi ...200314612229
ochrobactrum anthropi bacteraemia. 200314723384
atypical 16s rrna gene copies in ochrobactrum intermedium strains reveal a large genomic rearrangement by recombination between rrn copies.ochrobactrum intermedium is an opportunistic human pathogen belonging to the alpha 2 subgroup of proteobacteria. the 16s rdna sequences of nine o. intermedium isolates from a collection of clinical and environmental isolates exhibited a 46-bp insertion at position 187, which was present in only one sequence among the 82 complete or partial 16s rdna sequences of ochrobactrum spp. available in data banks. reverse transcription-pcr experiments showed that the 46-bp insertion remained in the 16s rrn ...200312700269
identification of a novel alpha-proteobacterium causing bacteraemia in an immunocompetent patient.an 89-year male with pyrexia and suspected bacteremia was admitted to hospital, where a gram-negative rod was identified from blood culture. the organism was difficult to identify phenotypically and the resulting sequencing of a 559 bp section of the 16s rrna gene did not have a high homology score (>97.0%) with any deposited genbank accession numbers and hence was not able to be assigned to a species within any genus. given that the isolate was a member of the alpha subclass of the proteobacter ...200312860153
septic shock caused by ochrobactrum anthropi in an otherwise healthy host.reported is a case of life-threatening septic shock that occurred in an otherwise healthy host after administration of a peripheral venous infusion of a solution contaminated with ochrobactrum anthropi, an unusual human pathogen. the rapid onset of shock may have been due to a large inoculum caused by nonsterile practices at the time of reconstitution.200312624082
nitrite reductase genes in halobenzoate degrading denitrifying bacteria.abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ...200319719666
isolation and identification of a novel strain of the genus ochrobactrum with phenol-degrading activity.a bacterial strain as1 belonging to the genus ochrobactrum, was isolated from an enriched phenol-activated sludge in egypt. this strain grew aerobically on phenol as the sole carbon source using the meta-cleavage pathway at high phenol-degrading rates compared with those in a previous report.200316233528
bacterial reduction of toxic cr (vi) into cr (iii).two chromium-resistant bacterial strains crt-1 and crt-13, which can tolerate k2cro4 up to 40 mg x ml(-1) on nutrient agar, 25 mg x ml(-1) k2 cro4 in nutrient broth, and up to 10 mg x ml(-1) in acetate-minimal media, were used in this study. on the basis of 16s rrna, strain crt-1 was identified as ochrobactrum intermedium and crt-13 as brevibacterium sp.. uptake of chromate was greater in living cells than in heat-killed cells. ochrobactrum intermedium crt-1 reduced 73% and 41% of cr(v) while br ...200415974008
acquisition of an agrobacterium ri plasmid and pathogenicity by other alpha-proteobacteria in cucumber and tomato crops affected by root mat.root mat of cucumbers and tomatoes has previously been shown to be caused by agrobacterium radiobacter strains harboring a root-inducing ri plasmid (pri). nine other pri-harboring alpha-proteobacteria have subsequently been isolated from root mat-infected crops. fatty acid profiling and partial 16s rrna sequence analysis identified three of these strains as being in the genus ochrobactrum, five as being in the genus rhizobium, and one as being in the genus sinorhizobium: an in vitro pathogenicit ...200415128532
occurrence and potential diagnostic applications of serological cross-reactivities between brucella and other alpha-proteobacteria.agrobacterium, sinorhizobium, and ochrobactrum are genera closely related to brucella but, in contrast to the latter, are not pathogenic for humans and animals. we studied by an indirect enzyme-linked immunosorbent assay (elisa) the reactivities of brucellosis sera against cytosolic (cyt) and membrane (ma) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of these cross-reactions for the diagnosis of brucellosis in humans, sheep, cows, and dogs. canine infecti ...200415358645
a novel symbiotic nitrogen-fixing member of the ochrobactrum clade isolated from root nodules of acacia mangium.ten strains of root nodule bacteria were isolated from the nodules of acacia mangium grown in the philippines and thailand. partial sequences (approx. 300 bp) of the 16s rrna gene of each isolate were analyzed. the nucleotide sequences of strain dasa 35030 indicated high homology (>99%) with members of the genus ochrobactrum in brucellaceae, although the sequences of other isolates were homologous to those of two distinct genera bradyrhizobium and rhizobium. the strain dasa 35030 was strongly su ...200415057707
s-stereoselective piperazine-2-tert-butylcarboxamide hydrolase from pseudomonas azotoformans iam 1603 is a novel l-amino acid amidase.an amidase acting on (r,s)-piperazine-2-tert-butylcarboxamide was purified from pseudomonas azotoformans iam 1603 and characterized. the enzyme acted s-stereoselectively on (r,s)-piperazine-2-tert-butylcarboxamide to yield (s)-piperazine-2-carboxylic acid. n-terminal and internal amino acid sequences of the enzyme were determined. the gene encoding the s-stereoselective piperazine-2-tert-butylcarboxamide amidase was cloned from the chromosomal dna of the strain and sequenced. analysis of 2.1 kb ...200415066172
endophytic colonization ability of two deep-water rice endophytes, pantoea sp. and ochrobactrum sp. using green fluorescent protein reporter.colonization ability of the two endophytic bacteria, isolated from surface sterilized seeds of jaisurya variety of deep-water rice viz., pantoea sp. and ochrobactrum sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp). confocal laser scanning microscopy (clsm) of hydroponically grown seedlings of jaisurya rice, inoculated with gfp-tagged endophytes, revealed that both pantoea sp. and ochrobactrum sp. colonized the intercellular s ...200415104142
glutathione-dependent biotransformation of the fungicide chlorothalonil.a gene responsible for the chlorothalonil biotransformation was cloned from the chromosomal dna of ochrobactrum anthropi sh35b, capable of efficiently dissipating the chlorothalonil. the gene encoding glutathione s-transferase (gst) of o. anthropi sh35b was expressed in escherichia coli, and the gst was subsequently purified by affinity chromatography. the fungicide chlorothalonil was rapidly transformed by the gst in the presence of glutathione. lc-ms analysis supported the formation of mono-, ...200415212468
prosthetic mitral valve endocarditis due to ochrobactrum anthropi: case report.we describe a case of infective endocarditis in a prosthetic mitral valve due to ochrobactrum anthropi. although o. anthropi is an emerging pathogen in immunocompromised patients, infections with the bacterium have very rarely been documented in healthy hosts, and endocarditis is rare. to our knowledge, only two cases of o. anthropi endocarditis have been reported in the medical literature.200415243121
interaction of endotoxins with toll-like receptor 4 correlates with their endotoxic potential and may explain the proinflammatory effect of brucella spp. lps.endotoxins displaying differences in the chemical structure of their lipid a were used to induce the expression of chemokines in the human monocytic thp-1 cell line. lps from two enterobacterial species such as escherichia coli and yersinia enterocolitica induced mrna expression of ifn-gamma-inducible protein (ip)-10, macrophage-inflammatory protein (mip)-1alpha, mip-1beta, monocyte chemoattractant protein (mcp)-1 and il-8. lps from the non-enterobacterial genera brucella and ochrobactrum induce ...200415339879
expression of glutathione s-transferase and peptide methionine sulphoxide reductase in ochrobactrum anthropi is correlated to the production of reactive oxygen species caused by aromatic substrates.peptide methionine sulphoxide reductase (msra) and glutathione s-transferases (gsts) are considered as detoxification enzymes. in the xenobiotics-degrading bacterium ochrobactrum anthropi the two enzymes are co-induced by toxic concentrations of aromatic substrates such as phenol and 4-chlorophenol. in aerobic organisms, degradation of aromatic substrates by mono- and dioxygenases leads to a generation of oxidative stress that causes the occurrence of reactive oxygen species (ros). a capillary e ...200415598526
paraquat resistance of transgenic tobacco plants over-expressing the ochrobactrum anthropi pqra gene.transgenic tobacco plants over-expressing the ochrobactrum anthropi pqra gene, which encodes a membrane transporter mediating resistance to paraquat, were generated. transgenic plants displayed higher resistance against paraquat than wild-type plants, as estimated by plant viability, ion leakage and chlorophyll loss, but no resistance against other active oxygen generators, such as h2o2 and menadione. moreover, lower levels of paraquat accumulated in transgenic plants, compared to wild-type plan ...200415604769
comparative study of cr(vi) uptake and reduction in industrial effluent by ochrobactrum intermedium and brevibacterium sp.two chromium-resistant bacterial strains, crt-1 and crt-13, tolerant up to 40 mg k2cro4 ml(-1) on nutrient agar, 25 mg ml(-1) in nutrient broth, and up to 10 mg ml(-1) in acetate-minimal media, were identified as ochrobactrum intermedium and brevibacterium sp., respectively, on the basis of 16s rrna gene sequencing. uptake of chromate was greater in living cells than in heat-killed on dried cells. crt-1 reduced 82%, 28% and 16% of cr(vi) at 100, 500, and 1000 microg ml(-1) after 24 h while crt-1 ...200415604809
ochrobactrum tritici strain 5bvl1 - characterization of a cr(vi)-resistant and cr(vi)-reducing strain.bacterial strain 5bvl1, isolated from a chromium-contaminated wastewater treatment plant and identified as ochrobactrum tritici, was resistant to a broad range of antibiotics, to cr(vi), ni(ii), co(ii), cd(ii), and zn(ii), and was able to grow in the presence of 5% nacl and within the ph range 4-10. characterization showed that strain 5bvl1 could be considered a halotolerant and alkalitolerant microorganism resistant to high concentrations of cr(vi). this strain was able to grow aerobically in u ...200415644923
involvement of linear plasmids in aerobic biodegradation of vinyl chloride.pseudomonas putida strain aj and ochrobactrum strain td were isolated from hazardous waste sites based on their ability to use vinyl chloride (vc) as the sole source of carbon and energy under aerobic conditions. strains aj and td also use ethene and ethylene oxide as growth substrates. strain aj contained a linear megaplasmid (approximately 260 kb) when grown on vc or ethene, but it contained no circular plasmids. while strain aj was growing on ethylene oxide, it was observed to contain a 100-k ...200415466555
use of amplified fragment length polymorphism to identify and type brucella isolates of medical and veterinary interest.amplified fragment length polymorphism (aflp) is a whole-genome fingerprinting method that relies on the selective pcr amplification of restriction fragments. the potential of this approach for the discrimination of brucella isolates at the species and intraspecies level was assessed. a number of different combinations of restriction enzymes and selective primers were examined, and one, using ecori and msei with additional selective tc bases on the msei primer, was selected for full assessment a ...200515695677
dom-fold: a structure with crossing loops found in dmpa, ornithine acetyltransferase, and molybdenum cofactor-binding domain.understanding relationships between sequence, structure, and evolution is important for functional characterization of proteins. here, we define a novel dom-fold as a consensus structure of the domains in dmpa (l-aminopeptidase d-ala-esterase/amidase), oat (ornithine acetyltransferase), and mocobd (molybdenum cofactor-binding domain), and discuss possible evolutionary scenarios of its origin. as shown by a comprehensive structure similarity search, dom-fold distinguished by a two-layered beta/al ...200515937278
bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites.in-situ bioremediation of petroleum waste sludge in landfarming sites of motor oil hellas (petroleum refinery) was studied by monitoring the changes of the petroleum composition of the waste sludge, as well as the changes in the structure of the microbial community, for a time period of 14 months. the analyses indicated an enhanced degradation of the petroleum hydrocarbons in the landfarming areas. a depletion of n-alkanes of approximately 75-100% was obtained. marked changes of the microbial co ...200515730027
[ochrobactrum anthropi infectious endocarditis in an immunocompetent patient]. 200515743587
nodulation of lupinus albus by strains of ochrobactrum lupini sp. nov.the nodulation of legumes has for more than a century been considered an exclusive capacity of a group of microorganisms commonly known as rhizobia and belonging to the alpha-proteobacteria. however, in the last 3 years four nonrhizobial species, belonging to alpha and beta subclasses of the proteobacteria, have been described as legume-nodulating bacteria. in the present study, two fast-growing strains, lup21 and lup23, were isolated from nodules of lupinus honoratus. the phylogenetic analysis ...200515746334
genotyping of ochrobactrum spp. by aflp analysis.aflp was used to analyze the genetic diversity among ochrobactrum strains. aflp patterns showed a great genomic variability that separated the samples into three distinct clusters. ochrobactrum intermedium was found to be closely related to brucella abortus s99.200515774899
dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase.amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ...200515913357
chromium-resistant bacteria and cyanobacteria: impact on cr(vi) reduction potential and plant growth.two chromium-resistant bacterial strains, bacillus cereus s-6 and ochrobactrum intermedium crt-1, and two cyanobacterial strains, oscillatoria sp. and synechocystis sp., were used in this study. at initial chromate concentrations of 300 and 600 microg k2cro4 ml(-1), and an inoculum size of 9.6 x 10(7) cells ml(-1), b. cereus s-6 completely reduced cr(vi), while o. intermedium crt-1 reduced cr(vi) by 98% and 70%, respectively after 96 h. at 100 microg k2cro4 ml(-1), synechocystis sp. mk(s) and os ...200515926088
a dmpa-homologous protein from pseudomonas sp. is a dipeptidase specific for beta-alanyl dipeptides.we have determined the nucleotide sequence of a dna fragment covering the flanking region of the r-stereoselective amidase gene, rama, from the pseudomonas sp. mci3434 genome and found an additional gene, bapa, coding for a protein showing sequence similarity to dmpa aminopeptidase from ochrobactrum anthropi lmg7991 (43% identity). the dmpa (called l-aminopeptidase d-ala-esterase/amidase) hydrolyzes alanine-p-nitroanilide, alaninamide, and alanine methylester with a preference for the d-configur ...200515955066
evaluation of ochrobactrum intermedium bacteremia in a patient with bladder cancer.it is generally believed that ochrobactrum anthropi is the only pathogenic species in the genus ochrobactrum. we report a case of presumptive ochrobactrum intermedium bacteremia in a patient with bladder cancer. identification of a rare species using rapid commercial panel could lead to an erroneous result as this clinical isolate was initially reported to be shewanella putrefaciens. the patient was successfully treated with imipenem and ciprofloxacin.200516168615
specificity inversion of ochrobactrum anthropi d-aminopeptidase to a d,d-carboxypeptidase with new penicillin binding activity by directed mutagenesis.the serine penicillin-recognizing proteins have been extensively studied. they show a wide range of substrate specificities accompanied by multidomain features. their adaptation capacity has resulted in the emergence of pathogenic bacteria resistant to beta-lactam antibiotics. the most divergent enzymatic activities in this protein family are those of the ochrobactrum anthropi d-aminopeptidase and of the streptomyces r61 d,d-carboxypeptidase/transpeptidase. with the help of structural data, we h ...200516131658
x-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster.the orf6 gene from the clavulanic acid biosynthesis gene cluster encodes an oat (ornithine acetyltransferase). similar to other oats the enzyme has been shown to catalyse the reversible transfer of an acetyl group from n-acetylornithine to glutamate. oats are ntn (n-terminal nucleophile) enzymes, but are distinct from the better-characterized ntn hydrolase enzymes as they catalyse acetyl transfer rather than a hydrolysis reaction. in the present study, we describe the x-ray crystal structure of ...200515352873
diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera pseudaminobacter, achromobacter, brucella, and ochrobactrum. southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from plesiomonas sp. strain m6 and were all located on the chromosome. gene libraries were constructed from genomic dna of each of the 7 organophosphorus pesticide-degrading bacter ...200515980896
[isolation and identification of a strain dn2 degrading nicotine].a bacterial strain which was able to utilize nicotine as its sole carbon source was isolated from soil in which tobacco had grown at sanming region in fujian province and named as dn2. upon chemotaxonomic characterization and phylogenetic inference based 16s rdna analysis, the strain dn2 was identified as a-proteobacteria, ochrobactrum intermedium. for dn2 degrading nicotine, the optimal ph and temperature is 6.5, 30 degrees c respectively. it can tolerate high-concentration of nicotine up to 40 ...200515989256
['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp].gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ...200515991478
bacterial cr(vi) reduction concurrently improves sunflower (helianthus annuus l.) growth.four cr(vi)-reducing bacterial strains (ochrobactrum intermedium, crt-2, crt-3 and crt-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (helianthus annuus var sf-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg crcl3 or k2cro4 ml(-1)). severe reduction (20%) in seed germination was observed in cr(vi) stress. plant height decreased (36%) with cr(vi) when compared with chromium-free control, whi ...200516091890
molecular and phenotypic features for identification of the opportunistic pathogens ochrobactrum spp.among the six species characterized within the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium are currently reported as opportunistic pathogens in humans. since the species identification is mainly based on 16s rdna analysis, the aim of this study was to search for other characteristics useful for ochrobactrum species discrimination. ribotyping, morphological and biochemical analyses, and antimicrobial susceptibility testing were performed for a panel of 35 clinical isola ...200516157548
[duplicate publication: infectious endocarditis by ochrobactrum anthropi on immunocompetent patient]. 200516185575
the rhizosphere as a reservoir for opportunistic human pathogenic bacteria.during the last years, the number of human infections caused by opportunistic pathogens has increased dramatically. one natural reservoir of opportunistic pathogens is the rhizosphere, the zone around roots that is influenced by the plant. due to a high content of nutrients, this habitat is a 'microbial hot-spot', where bacterial abundances including those with strong antagonistic traits are enhanced. various bacterial genera, including burkholderia, enterobacter, herbaspirillum, ochrobactrum, p ...200516232283
l-selective amidase with extremely broad substrate specificity from ochrobactrum anthropi ncimb 40321.an industrially attractive l-specific amidase was purified to homogeneity from ochrobactrum anthropi ncimb 40321 wild-type cells. the purified amidase displayed maximum initial activity between ph 6 and 8.5 and was fully stable for at least 1 h up to 60 degrees c. the purified enzyme was strongly inhibited by the metal-chelating compounds edta and 1,10-phenanthroline. the activity of the edta-treated enzyme could be restored by the addition of zn2+ (to 80%), mn2+ (to 400%), and mg2+ (to 560%). s ...200516332774
bacterial communities in petroleum oil in stockpiles.bacterial communities in crude-oil samples from japanese oil stockpiles were investigated by 16s rrna gene cloning, followed by denaturing gradient gel electrophoresis (dgge) analysis. 16s rrna genes were successfully amplified by pcr after isooctane treatment from three kinds of crude-oil sample collected at four oil stockpiles in japan. dgge profiles showed that bacteria related to ochrobactrum anthropi, burkholderia cepacia, stenotrophomonas maltophilia, propionibacterium acnes, and brevundim ...200516233771
delta-(d -alpha-aminoadipoyl)-cleaving amidase of ochrobactrum anthropi.ochrobactrum anthropi cleaved the delta-(d-alpha-aminoadipoyl)-side chain from delta-(d-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin c analogue. in whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. o. anthropi possesses also gamma-d-glutamyltranspeptidase activity, 8 nkat g(-1) dry cell wt, the likely cause of delta-(d-alpha-aminoadipoyl)-cleavage.200516328990
pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus ochrobactrum.the alpha-proteobacterial genus ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. the genomes of nine strains of ochrobactrum anthropi and eight strains of ochrobactrum intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of i-ceui dige ...200515995979
[infective endocarditis due to ochrobactrum anthropi]. 200515970175
beneficial role of hydrophytes in removing cr(vi) from wastewater in association with chromate-reducing bacterial strains ochrobactrum intermedium and brevibacterium.this study deals with the use of three chromium-resistant bacterial strains (ochrobactrum intermedium crt-1, brevibacterium crt-13, and crm-1) in conjunction with eichornia crassipes for the removal of toxic chromium from wastewater. bacterial strains resulted in reduced uptake of chromate into inoculated plants as compared to noninoculated control plants. in the presence of different heavy metals, chromium uptake into the plants was 28.7 and 7.15% less at an initial k2cro4 concentration of 100 ...200516463540
differential inductions of tnf-alpha and igtp, iigp by structurally diverse classic and non-classic lipopolysaccharides.the innate immune system recognizes microbes by characteristic molecules like the gram-negative lipopolysaccharide (lps). lipid a (the lps bioactive moiety) signals through toll-like receptors (tlrs) to induce pro-inflammatory molecules and small gtpases of the p47 family involved in intracellular pathogen control. we tested tnf-alpha and p47-gtpase induction in macrophages using classical lpss [lipid as with glucosamine backbones, ester- and amide-linked c14:0(3-oh) and c12 to c16 in acyloxyacy ...200616469053
involvement of coenzyme m during aerobic biodegradation of vinyl chloride and ethene by pseudomonas putida strain aj and ochrobactrum sp. strain td.the involvement of coenzyme m in aerobic biodegradation of vinyl chloride and ethene in pseudomonas putida strain aj and ochrobactrum sp. strain td was demonstrated using pcr, hybridization, and enzyme assays. the results of this study extend the range of eubacteria known to use epoxyalkane:coenzyme m transferase.200616672529
growth stimulatory effect of ochrobactrum intermedium and bacillus cereus on vigna radiata plants.this study assessed the plant growth-promoting ability of the bacterial strains ochrobactrum intermedium (isolate crt-1) and bacillus cereus (isolate s-6).200616965380
alkane utilization by rhodococcus strain ntu-1 alone and in its natural association with bacillus fusiformis l-1 and ochrobactrum sp.linear (n-hexadecane) and branched (pristane) alkanes were degraded by a mixed culture isolated from an oil-contaminated field. the degradation was accompanied by formation of biofloccules. the culture was composed of rhodococcus strain ntu-1, bacillus fusiformis l-1, and ochrobactrum sp. rhodococcus strain ntu-1 carried out the degradation of the alkane via a hydroxylase. bacillus fusiformis l-1 and ochrobactrum sp. did not degrade the alkanes but aided the flocculation by forming more rigid ba ...200617022676
differences in microbial activity and microbial populations of peat associated with suppression of damping-off disease caused by pythium sylvaticum.the microbiological characteristics associated with disease-suppressive peats are unclear. we used a bioassay for pythium sylvaticum-induced damping-off of cress seedlings to identify conducive and suppressive peats. microbial activity in unconditioned peats was negatively correlated with the counts of p. sylvaticum at the end of the bioassay. denaturing gradient gel electrophoresis (dgge) profiling and clone library analyses of small-subunit rrna gene sequences from two suppressive and two cond ...200617021192
establishment of a gene expression system in ochrobactrum anthropi.genetic studies of ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. we constructed a set of vectors containing several promoters and a his tag fusion in the n terminus to facilitate protein detection and purification. the new vectors should significantly enhance the genetic manipulation and characterization of o. anthropi.200617021239
pelvic abscess due to ochrobactrum intermedium [corrected] in an immunocompetent host: case report and review of the literature.ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. we report a case of pelvic abscess with o. intermedium [corrected] after a routine appendectomy in an immunocompetent patient and review the literature on o. intermedium [corrected] infection in patients with normal immune function.200616517927
herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops.acetohydroxyacid synthase (ahas) inhibitors interfere with branched-chain amino acid biosynthesis by inhibiting ahas. glyphosate affects aromatic amino acid biosynthesis by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (epsps). glufosinate inhibits glutamine synthetase and blocks biosynthesis of glutamine. ahas gene variants that confer tolerance to ahas inhibitors have been discovered in plants through selection or mutagenesis. imidazolinone-tolerant crops have been commercialized base ...200616547651
genotyping of ochrobactrum anthropi by reca-based comparative sequence, pcr-rflp, and 16s rrna gene analysis.a reca-pcr restriction fragment length polymorphism assay was developed to study intraspecies variation among ochrobactrum anthropi. primers deduced from the known reca gene sequence of the genetically closely related genus brucella allowed the specific amplification of a 1065 bp reca fragment from each of the 38 o. anthropi and the eight brucella strains investigated. reca was also amplified from the type strains of o. intermedium, o. tritici, and o. lupini but could not be generated from o. gr ...200616553826
ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in india.a non-pigmented, motile, gram-negative bacterium designated mtcc 4195(t) was isolated from surface-sterilized seeds and plant tissue from deep-water rice (oryza sativa) cultivated in suraha tal lake in northern india. this isolate was shown to reinfect and colonize deep-water rice endophytically. the highest level of 16s rrna sequence similarity (96.8 %) to strain mtcc 4195(t) was shown by ochrobactrum gallinifaecis dsm 15295(t). strain mtcc 4195(t) utilized gamma-hydroxybutyric acid, adonitol, ...200616825649
description of pseudochrobactrum gen. nov., with the two species pseudochrobactrum asaccharolyticum sp. nov. and pseudochrobactrum saccharolyticum sp. nov.two gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (ccug 46016(t) and ccug 33852(t)), isolated from a knee aspirate of a 66-year-old man and an industrial glue, respectively, were studied for their taxonomic position. on the basis of chemotaxonomic data [i.e. major ubiquinone (q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine) and major fatty acids (c(18 : 1)omega7c and c(19 : 0) cyclo omega8c)] and 16s rrna gen ...200616902015
complete mineralisation of dimethylformamide by ochrobactrum sp. dgvk1 isolated from the soil samples collected from the coalmine leftovers.a bacterial strain dgvk1 capable of using n,n-dimethylformamide (dmf) as sole source of carbon and nitrogen was isolated from the soil samples collected from the coalmine leftovers. the molecular phylogram generated using the complete sequence of 16s rdna of the strain dgvk1 showed close links to the bacteria grouped under brucellaceae family that belongs to alphaproteobacteria class. specifically, the 16s rdna sequence of strain dgvk1 has shown 97% similarity to ochrobactrum anthropi lmg 3331 ( ...200616211382
ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes.the use of two-phase centrifugal decanters has been widely adopted in the olive oil extraction industry in order to reduce the huge quantities of wastewaters produced during the traditional three-phase extraction process. the resulting sludge-like byproduct, widely known as "alpeorujo", has a ph of 4-6, low water activity (a(w)) and high phytotoxicity. addition of ca(oh)(2) to alpeorujo, which is commonly performed at the olive oil mill to handle disposal problems related to acidic ph and odor e ...200616307869
comparative sequence analysis of the internal transcribed spacer 1 of ochrobactrum species.the internal 16s/23s rdna (rrs/rrl) internal spacer region 1 (its1) of 54 ochrobactrum strains and close relatives was analysed. separation of its1 containing pcr products by gel-electrophoresis, dgge, cloning and sequencing revealed its1 length and sequence heterogeneity. we found up to 5 different allelic its1 stretches within a single strain (ochrobactrum intermedium lmg 3301t), and 2-3 different its1 alleles in o. tritici. within its1, its1c, being part of the conserved double-stranded rrn p ...200616352412
the effects of salinity and other factors on nitrite reduction by ochrobactrum anthropi 49187.the nitrite reductase (nir) gene was cloned from ochrobactrum anthropi 49187 and found to contain an open reading frame of 1131 nucleotides, encoding a polypeptide of 376 amino acids. the o. anthropi nir gene encodes a copper-type dissimilatory reductase based on sequence homology with other genes. the polypeptide product is predicted to form a trimeric holoenzyme of 37 kda subunits based on molecular weight estimates of extracts in activity gels. expression of the enzyme is up-regulated by nitr ...200616463313
recurrent outbreaks of root mat in cucumber and tomato are associated with a monomorphic, cucumopine, ri-plasmid harboured by various alphaproteobacteria.recurrent outbreaks of root mat have occurred in the uk and france in cucumber and tomato. root mat is caused by bacterial strains harbouring a ri-plasmid (pri). fifteen root mat-associated (rma) cucumopine pri were analysed by pcr-restriction fragment length polymorphism (rflp) and southern blotting. these pri were harboured by agrobacterium biovar 1 strains isolated during a 1970s outbreak of root mat in uk soil grown cucumber, and also by agrobacterium biovar 1, ochrobactrum, rhizobium and si ...200616630268
structure and membrane affinity of new amphiphilic siderophores produced by ochrobactrum sp. sp18.the coastal alpha-proteobacterium ochrobactrum sp. sp18 produces a suite of three citrate-derived, cell-associated amphiphilic siderophores, ochrobactins a-c. the ochrobactins are composed of a citric acid backbone amide-linked to two lysine residues. each epsilon-amine of lysine is hydroxylated and acylated forming two hydroxamic acid moieties. one of the acylated appendages of each ochrobactin is (e)-2-decenoic acid. the other acylated appendages for ochrobactins a-c are (e)-2-octenoic acid, o ...200616791646
percutaneous balloon pericardiotomy for the treatment of infected pericardial effusion with tamponade.percutaneous balloon pericardiotomy has been used as an alternative for surgically created pericardial window mainly for the management of malignant pericardial effusions in critically ill patients. we describe a patient with purulent pericardial effusion and cardiac tamponade who was treated successfully and without complications with percutaneous balloon pericardiotomy.200616816447
genetic and phenotypic microdiversity of ochrobactrum spp.the diversity of ochrobactrum anthropi, ochrobactrum intermedium, ochrobactrum tritici and ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. o. anthropi was isolated both from soil and from the rhizoplane, o. intermedium and grignonense only from bulk soil, and o. tritici only from the wheat rhizoplane. on the genetic level, the immunotrapped isolates and a number of strains from culture collection mainly of clinical origin were compared with rep-pcr pro ...200616629756
optimization of a medium for enhancing nicotine biodegradation by ochrobactrum intermedium dn2.to optimize a medium for nicotine degradation by ochrobactrum intermedium dn2 in presence of yeast extract, glucose and tween 80 using response surface methodology (rsm).200616907819
isolation and characterization of a bacterial strain of the genus ochrobactrum with methyl parathion mineralizing activity.to isolate and characterize a methyl parathion (mp)-mineralizing bacterium, and to elucidate the degradative pathway of mp and localize the responsible degrading genes.200617040221
multidimensional proteomic analysis of the soluble subproteome of the emerging nosocomial pathogen ochrobactrum anthropi.we report the first large-scale gel-free proteomic analysis of the soluble subproteome of the emerging pathogen ochrobactrum anthropi. utilizing our robust offline multidimensional protein identification protocol, a total of 57 280 peptides were initially identified utilizing automated ms/ms analysis software. we describe our investigation of the heuristic protein validation tool provalt and demonstrate its ability to increase the speed and accuracy of the curation process of large-scale proteom ...200617081066
an outbreak of extrinsic alveolitis at a car engine plant.twelve workers from a car engine-manufacturing plant presented with extrinsic allergic alveolitis (eaa), with heterogeneous clinical, radiological and pathological findings. they were exposed to metalworking fluids (mwf) that cooled, lubricated and cleaned the machines.200617095570
ochrobactrum anthropi endocarditis and septic shock in a patient with no prosthetic valve or rheumatic heart disease: case report and review of the literature.although ochrobactrum anthropi is an opportunistic pathogen in immunocompromised patients, it is increasingly being recognized to be a causative agent in healthy hosts. in this paper, we report a case of o. anthropi endocarditis and septic shock in a patient who had no prosthetic valve or rheumatic heart disease, in contrast to previous reports.200616936348
detection and characterization of bacteria from the potato rhizosphere degrading n-acyl-homoserine lactone.quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium pectobacterium carotovorum subsp. carotovorum. the signal molecules involved in quorum sensing in p. carotovorum subsp. carotovorum belong to the group of n-acyl homoserine lactones (ahls). in our study, we screened bacteria isolated from the potato rhizosphere for the ability to degrade ahls produced by p. carotovorum subsp. carotovorum. six isolates able to degrade ahls were selected for ...200617110970
cloning, expression and characterization of immunogenic aminopeptidase n from brucella melitensis.a 97-kda purified aminopeptidase n (pepn) of brucella melitensis was previously identified to be immunogenic in humans. the b. melitensis pepn gene was cloned, expressed in escherichia coli and purified by affinity chromatography. the recombinant pepn (rpepn) exhibited the same biochemical properties, specificity and susceptibility to inhibitors as the native pepn. rpepn was evaluated as a diagnostic antigen in an indirect enzyme-linked immunosorbent assay (elisa) using sera from patients with a ...200617064279
[construction of a versatile degrading bacteria pseudomonas putida kt2440-dop and its degrading characteristics].triazophos is a kind of organophosphorous pesticide which was widely used by farmers all over the world in 1990's. it is effective in controlling pesticide but harmful to human beings. bioremediation is an effective and economic method to treat environment that has been polluted by hazardous organic compounds, so researchers paid much attention in this area. most of which focused on isolating functional bacteria, studying its degrading mechanism, and cloning degradation-related genes. mp-4 was i ...200617172025
enzymatic degradation of beta- and mixed alpha,beta-oligopeptides.one of the main and most astonishing characteristics of peptides comprised of beta-amino acids with proteinogenic side chains is their extraordinarily high stability towards enzymatic degradation. so far, only certain microbial enzymes have been shown to cleave n-terminal beta(3)-homoamino acid residues from peptides. in this work, the l-aminopeptidase-d-amidase/esterase (dmpa) from ochrobactrum anthropi lmg7991 is compared to two closely related beta-peptidyl aminopeptidases (bapa), which origi ...200617193247
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