Publications

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draft genome sequence of moraxella bovoculi strain 237t (atcc baa-1259t) isolated from a calf with infectious bovine keratoconjunctivitis.moraxella bovoculi is a recently identified species, recovered from the bovine eye, which is under investigation as an etiological agent of infectious bovine keratoconjunctivitis. a draft genome sequence of the moraxella bovoculi type strain 237(t) has been determined to identify features that may be important during host colonization.201424970830
moraxella lacunata infection associated with septicemia, endocarditis, and bilateral septic arthritis in a patient undergoing hemodialysis: a case report and review of the literature.we report the first case of both endocarditis and bilateral septic arthritis in a patient caused by moraxella lacunata and successful management of the infection with antimicrobial therapy. the route of entry leading to bacteremia may have been the oral cavity given the poor oral hygiene of the patient as evidenced by bleeding gums. we hypothesize that the bacteremia led to septic arthritis and mitral valve infective endocarditis. in this case report, we also review the literature on m. lacunata ...201424462428
[undulant fever and autoimmune hemolytic anemia in a 20-year-old exchange student from jordan - the human brucellosis as an important differential diagnosis in migrants].a 20-year-old jordanian exchange student presents with recurrent fever, night sweats, cough, and swelling and redness around the ankle. physical examination further reveals bilateral ankle arthritis and painful cervical lymphadenopathy.201526625239
development of two real-time multiplex pcr assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections.the frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (lrti) is an important obstacle to antimicrobial stewardship. patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results are awaited, but, because these are often slow, negative, or inconclusive, de-escalation to narrow-spectrum agents rarely occurs in clinical practice. the aim of this study was to develop and evaluate two multiplex real- ...201525980353
bacterial spectrum and antibiotic resistance patterns of ocular infection: differences between external and intraocular diseases.this study aimed to compare the differences of microbial spectrum and antibiotic resistance patterns between external and intraocular bacterial infections in an eye hospital in south china. a total of 737 bacteria isolates from suspected ocular infections were included in this retrospective study covering the period 2010-2013. the organisms cultured from the ocular surface (cornea, conjunctiva) accounted for the majority of the isolates (82.77%, n = 610), followed by the intraocular (aqueous hum ...201526576294
a population-based acute meningitis and encephalitis syndromes surveillance in guangxi, china, may 2007-june 2012.acute meningitis and encephalitis (ame) are common diseases with the main pathogens being viruses and bacteria. as specific treatments are different, it is important to develop clinical prediction rules to distinguish aseptic from bacterial or fungal infection. in this study we evaluated the incidence rates, seasonal variety and the main etiologic agents of ame, and identified factors that could be used to predict the etiologic agents.201526633824
the delta high-density lipoprotein cholesterol ratio: a novel parameter for gram-negative sepsis.to study changes in blood lipid metabolism in sepsis patients, especially high-density lipoprotein cholesterol (hdl-c) changes in the diagnosis of sepsis and the type of bacteria involved.201627462492
the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members.the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members.201627448413
development of a simple clinical risk score for early prediction of severe dengue in adult patients.we aimed to develop and validate a risk score to aid in the early identification of laboratory-confirmed dengue patients at high risk of severe dengue (sd) (i.e. severe plasma leakage with shock or respiratory distress, or severe bleeding or organ impairment). we retrospectively analyzed data of 1184 non-sd patients at hospital presentation and 69 sd patients before sd onset. we fit a logistic regression model using 85% of the population and converted the model coefficients to a numeric risk sco ...201627138448
evaluation of the bruker maldi biotyper for identification of fastidious gram-negative rods.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has entered clinical laboratories, facilitating identification of bacteria. here, we evaluated the maldi biotyper (bruker daltonics) for the identification of fastidious gram-negative rods (gnr). three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. direct colony transfe ...201626659214
draft genome sequence of psychrobacter sp. ennn9_iii, a strain isolated from water in a polluted temperate estuarine system (ria de aveiro, portugal).the genus psychrobacter includes gram-negative coccobacilli that are non-pigmented, oxidase-positive, non-motile, psychrophilic or psychrotolerant, and halotolerant. psychrobacter strain ennn9_iii was isolated from water in a polluted temperate estuarine system, contaminated with hydrocarbons and heavy metals. the genome has a g + c content of 42.7%, 2618 open reading frames (orfs), three copies of the rrnas operon, and 29 trna genes. twenty-five sequences related to the degradation of aromatic ...201627114904
spectrum and sensitivity of bacterial keratitis isolates in auckland.background. the bacteria isolated from severe cases of keratitis and their antibiotic sensitivity are recognised to vary geographically and over time. objectives. to identify the most commonly isolated bacteria in keratitis cases admitted over a 24-month period to a public hospital in auckland, new zealand, and to investigate in vitro sensitivity to antibiotics. methods. hospital admissions for culture-proven bacterial keratitis between january 2013 and december 2014 were identified. laboratory ...201627213052
in vitro activity of pexiganan and 10 comparator antimicrobials against 234 isolates including 93 pasteurella spp. and 50 anaerobic bacteria recovered from animal bite wounds.animal bite wounds affect more than 5 million americans resulting in 300,000 emergency department visits, 10,000 hospitalizations and untold number of physician office visits annually. various forms of topical therapy are empirically self-employed by many patients prior to seeking medical attention. pexiganan, a 22-amino acid synthetic cationic analogue of peptide magainin ii, acts by selectively damaging bacterial cell membranes. we determined the mics for pexiganan and other antimicrobial agen ...201728373186
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