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microbial baeyer-villiger reaction of bicyclo[3.2.0]heptan-6-ones--a novel approach to sarkomycin a.racemic (1 alpha, 2 alpha, 5 alpha)- and (1 beta, 2 alpha, 5 beta)-2- bromobicyclo[3.2.0]heptan-6-one (rac-7, rac-10, respectively), (1 alpha, 2 alpha, 5 beta)- and (1 beta, 2 alpha, 5 beta)-2- benzyloxybicyclo[3.2.0]heptan-6-one (rac-15, rac-13, respectively), (1 beta, 2 alpha, 5 beta)-2-hydroxybicyclo[3.2.0]heptan-6-one (rac-17) and cis-bicyclo[3.2.0]hept-2-en-7-one (rac-18) were subjected to a microbial baeyer-villiger reaction by acinetobacter calcoaceticus ncib 9871. in each case both regio ...19947858964
molecular typing of acinetobacter baumannii-acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections.ribotype, biotype and resistance phenotype were used to characterize 37 acinetobacter baumannii-a. calcoaceticus complex isolates responsible for nosocomial infections in buenos aires. nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. by ribotyping isolates were classified into five different clones of a. baumannii biotype 2, 3 of a. baumannii biotype 9, and 3 of acinetobacter genospecies 13 ...19957867730
spontaneous mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase in acinetobacter calcoaceticus.bacteria containing spontaneous null mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase, were selected by exposure of an acinetobacter calcoaceticus strain to physiological conditions in which expression of the genes prevents growth. the parental bacterial strain exhibits high competence for natural transformation, and this procedure was used to characterize 94 independently isolated spontaneous mutations. four of the mutations were caused by integration of a newly id ...19957868609
degradation of propanil by bacterial isolates and mixed populations from a pristine lake.the microbial transformation rates of propanil, a commonly used herbicide, were investigated using water from a pristine lake in northeast georgia. microbial degradation rates were measured using natural water microflora, the natural water microflora amended with five bacterial species (aerobacter aerogenes, aeromonas hydrophila, acinetobacter calcoaceticus, proteus mirabilis, and aeromonas salmonicida) isolated from the same lake, and the five isolates individually. transformation rate constant ...19957874462
nucleotide sequences transferred by gene conversion in the bacterium acinetobacter calcoaceticus.exchange of nucleotide (nt) sequences between the catijf and pcaljf regions of the acinetobacter calcoaceticus chromosome appears to contribute to frequent repair of mutations, including removal of a tn5 insertion from pcaj. repaired nt sequences are the products of nonreciprocal genetic exchange. the length of donor catijf nt tracts recovered in repaired pcaijf dna ranged from less than 315 nt to more than 881 nt. this evidence does not distinguish gene conversion from natural transformation as ...19957883173
regulation of the hema gene during 5-aminolevulinic acid formation in pseudomonas aeruginosa.the general tetrapyrrole precursor 5-aminolevulinic acid is formed in bacteria via two different biosynthetic pathways. members of the alpha group of the proteobacteria use 5-aminolevulinic acid synthase for the condensation of succinyl-coenzyme a and glycine, while other bacteria utilize a two-step pathway from aminoacylated trna(glu). the trna-dependent pathway, involving the enzymes glutamyl-trna reductase (encoded by hema) and glutamate-1-semialdehyde-2,1-aminomutase (encoded by heml), was d ...19957883699
analysis of the interaction between autochthonous bacteria and packaging material in pvc-bottled mineral water.a study with about 10,000 bottles produced by a mineral water company was undertaken in order to identify the causal agent of an off-odour occurrence in the bottled water. some physiological attributes of the dominant species over an 8-month period, as well as their interaction with packaging material, were investigated. pseudomonas maltophilia, p. acidovorans, acinetobacter calcoaceticus var. lowffi, frequently associated with bottles having an off-odour, seemed to play a decisive role in the p ...19947921893
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...19947933531
[susceptibilities of glucose non-fermentative gram-negative bacilli to antibiotics].glucose non-fermentative gram-negative bacilli are important nosocomial pathogens. this study concerned with susceptibilities to antibacterial agents of strains of glucose non-fermentative gram-negative bacilli that were isolated from cultures of clinical materials at 123 hospital laboratories throughout japan from september to december of 1991. the tests for susceptibilities were performed according to the disk dilution method recommended by nccls. the following bacteria were tested: pseudomona ...19947933532
[laboratory and clinical studies on combined effects of fosfomycin plus sulbactam/cefoperazone for mixed infections of mrsa and pseudomonas aeruginosa].the time-lag/sequential/step by step chemotherapy by fosfomycin plus sulbactam/cefoperazone including a short time small dose of steroid was done for 27 severely infected patients suffered from various cancer or other severe basal diseases. and the staggered "intensive" chemotherapy with added arbekacin or vancomycin to the previous staggered chemotherapy was done against 13 severely infected patients having mrsas among their infecting pathogens. 1. bacteriological effects pseudomonas aeruginosa ...19947933542
properties of acinetobacter calcoaceticus reca and its contribution to intracellular gene conversion.the acinetobacter calcoaceticus pcaj and catj genes, nearly identical in dna sequence, differ in transcriptional control and are separated by more than 20 kb of chromosomal dna. the pcaj3125 mutation is repaired frequently in organisms containing the wild-type catj gene. this high-frequency repair is eliminated in strains lacking the catj gene, which suggests that recombination between the homologous catj and pcaj genes contributes to the high-frequency repair of the pcaj3125 mutation. we report ...19947934905
community-acquired bacteremias from tunneled central intravenous lines: results from studies of a single vendor.tunneled central intravenous catheters are a common method for rendering prolonged outpatient intravenous therapy. their safety, however, has not been well studied. we conducted a retrospective evaluation of bacteremias associated with tunneled central intravenous catheters managed by a single home health care vendor during a 1-year period. all catheters were inserted in the operating room under sterile conditions. to calculate total line days, the dates of catheter insertion and removal were ob ...19947943925
[degradation of oil derivatives by acinetobacter calcoaceticus mm5].this paper describes the isolation of microorganisms from polluted heating oil. the growth of one of them has been studied (acinetobacter calcoaceticus mm5) in several linear and branched hydrocarbons as well as the effect of its growth on commercial diesel oil. acinetobacter calcoaceticus mm5 is not capable of using glucose as its only source of carbon, and it needs the presence of nitrogen and phosphorus sources to degrade any petroleum by-product.19947946119
a gene of acinetobacter calcoaceticus bd413 encodes a periplasmic peptidyl-prolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth.downstream of the acinetobacter calcoaceticus esta gene, encoding a cell-bound esterase, an open reading frame (orf) was identified, which may encode a protein with a mass of 20.4 kda. this protein shows extensive similarity to both prokaryotic and eukaryotic peptidyl-prolyl cis-trans isomerases (ppiases) of the cyclophilin sub-class, especially to the periplasmic rotamase (rota) of escherichia coli. a putative signal sequence suggests that the product of the acinetobacter gene, we termed rota, ...19947948017
inducible cell lysis system for the study of natural transformation and environmental fate of dna released by cell death.two novel conditional broad-host-range cell lysis systems have been developed for the study of natural transformation in bacteria and the environmental fate of dna released by cell death. plasmid pdkl02 consists of lysis genes s, r, and rz from bacteriophage lambda under the control of the ptac promoter. the addition of inducer to escherichia coli, acinetobacter calcoaceticus, or pseudomonas stutzeri containing plasmid pdkl02 resulted in cell lysis coincident with the release of high amounts of ...19947961508
purification and properties of a novel type of malonate decarboxylase from acinetobacter calcoaceticus.a novel type of malonate decarboxylase which is induced and located in periplasm of acinetobacter calcoaceticus grown on malonate as sole carbon and energy source was purified to homogeneity. the purified 185-kda decarboxylase was found to be an oligomer composed of three different polypeptides in a ratio of 2:1:1, designated alpha (65 kda), beta (32 kda), and gamma (25 kda). optimum ph was 6.8, while pi was 6.39. the enzyme was highly specific for malonate with km 1.4 mm. this enzyme contained ...19947961952
[hydrophobic properties of acinetobacter calcoaceticus].evaluation of hydrophobic properties of 309 strains of acinetobacter calcoaceticus was performed by a sat method. among investigated strains cultured at 22 degrees c, 103 strains were autoaggregating, representing very strong hydrophobic properties. most of strains aggregated at concentration of 0.4-1.0 m (nh4)2so4, exhibiting strong hydrophobic activities. only 38 strains during culture at 22 degrees c exhibited hydrophilic surface properties. differences between strains of anitratus variety an ...19947967921
[adhesion of acinetobacter calcoaceticus to cheek epithelial cells].adhesive properties of 309 strains of acinetobacter calcoaceticus were determined toward cells of cheek epithelium obtained from 13 healthy women. most of strains adhered in number of > 0-20 bacterial cells for one epithelial cell. adherence of a. calcoaceticus to cheek epithelium was dependent not only of variety, strain and temperature of bacterial culture, but also of individual properties of epithelial cells obtained from donors. relation between hydrophobic properties and adherence to cheek ...19947967922
[clinical evaluation of sulbactam/cefoperazone for infections during the chemotherapy of hematologic malignancy].in 28 patients with hematologic malignancy, the clinical effect and the safety of sulbactam/cefoperazone (sbt/cpz) were studied on 45 episodes during granulocytopenic stage after antineoplastic chemotherapy. the overall efficacy rate obtained with sbt/cpz in combination with other drugs was 62%. the rates for sepsis, suspected sepsis and pneumonia were 67%, 94% and 38%, respectively. the efficacy in those cases that showed granulocyte count less than 300/microliters during the course of administ ...19947990260
l-carnitine via enzyme-catalyzed oxidative kinetic resolution.l-carnitine of high optical purity was prepared via kinetic resolution using a mutant strain of acinetobacter calcoaceticus atcc 39647. this organism preferentially metabolized the d-enantiomer of the racemate to furnish l-carnitine. recovery of l-carnitine was 93%, providing a total weight yield of 46.5% in 92% enantiomeric excess. the mode of degradation of carnitine was shown to proceed via a monooxygenase-catalyzed oxidative cleavage resulting in the formation of trimethylamine and malic aci ...19948000862
bactericidal activity of cefpirome (hr 810) against 513 gram-negative bacteria isolated from blood of septicemic patients.the profile of antibacterial activity of cefpirome was compared with that of nine other antimicrobial agents against 513 gram-negative bacteria isolated from septicemic patients. all strains were evaluated for their sensitivity by disc diffusion and broth dilution tests (mic and mbc). cefpirome was compared to cefazolin, cefuroxime, ceftazidime, ceftriaxone, aztreonam, imipenem, ticarcillin, tobramycin and ciprofloxacin. among the five cephalosporins tested in this study, cefpirome was the most ...19948002094
the pca-pob supraoperonic cluster of acinetobacter calcoaceticus contains quia, the structural gene for quinate-shikimate dehydrogenase.an 18-kbp acinetobacter calcoaceticus chromosomal segment contains the pcaijfbdkchg operon, which is required for catabolism of protocatechuate, and pobsra, genes associated with conversion of p-hydroxybenzoate to protocatechuate. the genetic function of the 6.5 kbp of dna between pcag and pobs was unknown. deletions in this dna were designed by removal of fragments between restriction sites, and the deletion mutations were introduced into a. calcoaceticus by natural transformation. the mutation ...19948002591
regulation of p-hydroxybenzoate hydroxylase synthesis by pobr bound to an operator in acinetobacter calcoaceticus.pobr is a transcriptional activator required for the expression of poba, the structural gene for p-hydroxybenzoate hydroxylase. the poba and pobr genes are divergently transcribed and separated by 134 bp in the acinetobacter calcoaceticus chromosome. primer extension analysis revealed that the poba transcript begins 22 bp upstream from the structural gene and the pobr transcript begins 69 bp upstream from the regulatory gene. this arrangement requires superimposition of the -10 base pair and -35 ...19948021213
inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate.the conversion of 2-chloro-cis,cis-muconate by muconate cycloisomerase from pseudomonas putida prs2000 yielded two products which by nuclear magnetic resonance spectroscopy were identified as 2-chloro- and 5-chloromuconolactone. high-pressure liquid chromatography analyses showed the same compounds to be formed also by muconate cycloisomerases from acinetobacter calcoaceticus adp1 and pseudomonas sp. strain b13. during 2-chloro-cis,cis-muconate turnover by the enzyme from p. putida, 2-chloromuco ...19948021223
comparative serum bactericidal activities of three doses of ciprofloxacin administered intravenously.the pharmacokinetics and serum bactericidal activities of three intravenous doses of ciprofloxacin were studied comparatively in 30 patients. single 200-, 300-, and 400-mg intravenous doses of ciprofloxacin were given over 30 min to 10 patients each, and serum samples were obtained at 0.5, 1, 2, 3, 4, 8, and 12 h after the start of the infusion. serum drug concentrations were determined by high-pressure liquid chromatography. pharmacokinetic parameters were estimated by using noncompartmental an ...19948031055
regulation of spvr gene expression of salmonella virulence plasmid pkdsc50 in salmonella choleraesuis serovar choleraesuis.the expression regulation of spvr, a regulatory gene on the virulence plasmid (pkdsc50) of salmonella choleraesuis serovar choleraesuis, was investigated by spvr-lacz translational fusion. the spvr gene was found to be positively regulated by its own product, the spvr protein, and this unusual positive autoregulation was repressed by the products of spva and spvb, virulence-associated genes present downstream from the spvr gene. amino acid sequence analysis revealed that the amino-terminal regio ...19948052129
contrasting patterns of evolutionary divergence within the acinetobacter calcoaceticus pca operon.the six enzymes required for catabolism of protocatechuate to succinate and acetylcoa are encoded by the pca genes in the gram-bacterium, acinetobacter calcoaceticus. the clustered a. calcoaceticus cat genes encode an analogous set of enzymes associated with the metabolic dissimilation of catechol. the nucleotide (nt) sequences of pcaijfb and pcak, reported here, complete evidence showing that all of the pca structural genes are tightly grouped in the order pcaijfbdkchg within a single operon. t ...19948063101
comparison of three typing methods in hospital outbreaks of acinetobacter calcoaceticus infection.during a period of 11 months acinetobacter baumanii was isolated from 27 patients and 21 environmental samples in the intensive care unit (icu) and in one surgical unit of a university hospital. the isolates were characterized by biotyping, antibiograms and plasmid profiles and compared with co-isolates. plasmid fingerprinting distinguished three outbreaks, whereas other typing methods were less sensitive and discriminatory. although plasmid profiles seem to be a simple and reproducible marker f ...19938097217
crystallization and preliminary x-ray analysis of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus.x-ray quality single crystals of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus were obtained by the hanging drop method. the intradiol dioxygenase crystallizes in the cubic space group i23 with unit cell dimensions a = b = c = 145.5 a. the dodecahedral crystals diffract to beyond 2.5 a resolution. the asymmetric unit contains one twelfth of the enzyme (alpha beta fe+3)12 complex.19948107118
community-acquired bacteraemic acinetobacter pneumonia with survival.a 65 year old man was admitted with segmental consolidation of the left upper lobe after having stayed in a hotel for 2 days. he deteriorated rapidly on conventional antibiotic therapy and required ventilatory support. acinetobacter calcoaceticus var. anitratus was grown from the sputum and blood cultures, which was treated with a combination of anti-pseudomonal agent, aminoglycoside and cotrimoxazole. he made a slow but remarkable recovery from the pneumonia. acinetobacter is a rare potentially ...19938121870
unusual g + c content and codon usage in catijf, a segment of the ben-cat supra-operonic cluster in the acinetobacter calcoaceticus chromosome.the nucleotide (nt) sequence of a 5.3-kb dna segment containing the gram- acinetobacter calcoaceticus catbcijfd operon is reported. this information completes determination of a 16-kb nt sequence containing the twelve ben and cat structural genes encoding enzymes required for catabolism of benzoate via the beta-ketoadipate pathway. many of these genes can be traced to a common ancestry with genes from other organisms containing dna with widely divergent g + c content. the a. calcoaceticus ben an ...19948125318
isolation, phenotypic characterization, and complementation analysis of mutants of methylobacterium extorquens am1 unable to synthesize pyrroloquinoline quinone and sequences of pqqd, pqqg, and pqqc.aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (pqq) as a prosthetic group. seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with pqq have been isolated in the facultative methanol utilizer methylobacterium extorquens am1. in addition, 12 previously isolated methanol oxidation mutants of m. extorquens am1 were shown to be able to grow on methanol in the prese ...19948132470
azide-resistant mutants in acinetobacter calcoaceticus a2 are defective in protein secretion.azide, an inhibitor of atpase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in acinetobacter calcoaceticus a2. two such mutants were isolated that were azide-resistant and defective in the general protein transport system. the mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40 degrees c. the existence of protein secretion mutants with a selectable phenotype may be useful for the g ...19948150267
nadp-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from acinetobacter calcoaceticus and saccharomyces cerevisiae.an nadp-dependent constitutive alcohol dehydrogenase that can oxidize hexan-1-ol was detected in several gram-positive and gram-negative eubacteria and in two yeasts. the enzyme was purified to homogeneity from acinetobacter calcoaceticus ncib 8250 and from saccharomyces cerevisiae d273-10b. the bacterial enzyme appears to be a tetramer of subunit m(r) 40,300 and the yeast enzyme appears to be a monomer of subunit m(r) 43,500. the n-terminal amino acid sequence of the bacterial enzyme has 34% id ...19948162187
combined therapy of granulocyte transfusions, intravenous opsonins and antibiotics for gram-negative pneumonia in neutropenic cancer patients.four patients with hematological malignancies, following bone marrow transplantation, who developed documented gram-negative [klebsiella pneumoniae (2), pseudomonas aeruginosa or acinetobacter calcoaceticus] pneumonia during absolute neutropenia, were treated with a combination of antimicrobial therapy, granulocyte transfusions, and high-dose intravenous immunoglobulin. the patients recovered following this regimen, including 2 who had septic shock and respiratory failure, necessitating intubati ...19948171936
udp-n-acetylglucosamine 1-carboxyvinyl-transferase from acinetobacter calcoaceticus.we have analyzed the sequence downstream of rpon from acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to udp-n-acetylglucosamine 1-carboxyvinyl-transferase (murz). multicopy plasmids encoding this enzyme conferred phosphomycin resistance to a. calcoaceticus. the polar effect of a rpon mutation on the phosphomycin resistance level suggests that murz is, in part, cotranscribed with rpon. these observations confirm that a. calcoaceticus repre ...19948181716
acquisition of apparent dna slippage structures during extensive evolutionary divergence of pcad and catd genes encoding identical catalytic activities in acinetobacter calcoaceticus.the pca operon from the gram- bacterium acinetobacter calcoaceticus encodes all of the enzymes required for catabolism of protocatechuate to common intermediary metabolites. this report presents the 2754-nucleotide (nt) sequence of a hindiii restriction fragment containing pcad, the 801-bp gene encoding beta-ketoadipate enol-lactone hydrolase i. the deduced primary structure of a. calcoaceticus pcad shares 44% amino acid (aa) sequence identity with the aligned primary structure of catd (beta-ket ...19948181753
[hemagglutinating properties of acinetobacter calcoaceticus].hemagglutinating activity was tested in 309 strains of acinetobacter calcoaceticus with fresh and tannine human, horse, sheep, bovine, chicken and guinea pig erythrocytes. determinations were performed with 0.1% d-mannose and without it, both in temperature 37 and 22 degrees c. hemagglutinating activity was exhibited by 75-85% of strains, more frequently cultured at 22 degrees c a. calcoaceticus var. anitratus equally frequently was agglutinating both types of erythrocytes. strains of a calcoace ...19938189805
in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin.in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin, were compared with those of cefpirome, ceftazidime and flomoxef. the advantages of in vitro activity of fk037 were as follows: (1) a broad-spectrum antibacterial activity, (2) the most potent activity (mic90: 25 micrograms/ml) of the cephalosporins tested against highly methicillin-resistant staphylococcus aureus (h-mrsa), (3) a strong activity against enterobacter spp. and citrobacter freundii resistant to t ...19948205935
rpon (sigma 54) is required for conversion of phenol to catechol in acinetobacter calcoaceticus.members of the sigma 54 protein family, encoded by rpon, are required for the transcription of genes associated with specialized metabolic functions. the ability to grow with phenol appears to be a specialized trait because it is expressed by few of the microorganisms that grow with catechol, the metabolic product of phenol monooxygenase. a mutation preventing the expression of phenol monooxygenase in the bacterial strain acinetobacter calcoaceticus ncib8250 was complemented by wild-type dna seg ...19948206826
isolation, characterization, and sequence analysis of cryptic plasmids from acinetobacter calcoaceticus and their use in the construction of escherichia coli shuttle plasmids.three cryptic plasmids have been discovered in acinetobacter calcoaceticus bd413. these three plasmids, designated pwm10 (7.4 kb), pwm11 (2.4 kb), and pwm12 (2.2 kb), exhibited extensive homology to one another, as shown by southern blot hybridization and restriction site analysis data, and also hybridized with three plasmids having slightly different sizes detected in a second strain, a. calcoaceticus bd4. plasmid pwm11 and a fragment of pwm10 were each subcloned into puc19, yielding plasmids p ...19938215356
s-adenosylhomocysteine hydrolase from acinetobacter calcoaceticus: purification and partial characterization.s-adenosylhomocysteine hydrolase (sahase) was purified to homogeneity from the gram negative strain acinetobacter calcoaceticus 501. the molecular weight of the native enzyme, estimated by gel permeation, was about 288 kda, while sodium dodecyl sulfate polyacrylamide gel electrophoresis yielded a relative molecular mass of 48 kda. the determination of the coenzyme content gave 4 mol of nad+ and 2 mol of nadh per mol of enzyme. the isoelectric point of native sahase was at ph 5.1. when assayed in ...19938220254
periplasmic aminopeptidases in acinetobacter calcoaceticus and pseudomonas aeruginosa.the greater part of the intracellular aminopeptidases in pseudomonas aeruginosa and acinetobacter calcoaceticus is soluble. the localization of aminopeptidases in the cells was examined using the osmotic shock method with some modifications. when the cells of a. calcoaceticus and p. aeruginosa of the logarithmic phase were subjected to an osmotic shock, all aminopeptidases investigated were mainly localized in the sucrose supernatants and in the periplasm. acid phosphatase as marker enzyme for p ...19938229672
an outbreak of gram-negative bacteremia traced to contaminated o-rings in reprocessed dialyzers.to investigate an outbreak of gram-negative bacteremia in an outpatient hemodialysis unit and to identify the source of contaminating bacteria and the route by which bacteria gained access to the bloodstream.19938239225
numerical classification and identification of acinetobacter genomic species.a total of 211 acinetobacter strains (representing all currently recognized genomic species) were tested for 329 biochemical characters. overall similarities of all strains were determined for 145 characters by numerical taxonomic techniques, the upgma algorithm and the s(sm)) and the s(j) coefficients as measures of similarity. seven clusters (two or more strains) and three unclustered strains were recovered at a similarity level of 80.0% (s(sm). at this level a complete correspondence between ...19938244904
infections caused by imipenem-resistant acinetobacter calcoaceticus biotype anitratus. 19938245560
growth-phase-dependent expression of the lipolytic system of acinetobacter calcoaceticus bd413: cloning of a gene encoding one of the esterases.acinetobacter calcoaceticus bd413, when grown in batch culture in nutrient broth, produces both extracellular lipase activity and cell-bound esterase activity during and after the transition between exponential growth and the stationary phase. from a library of a. calcoaceticus dna in escherichia coli, plasmids were isolated that enabled e. coli to grow on media with tributyrin as the sole carbon source. assays with model substrates classified the product of the cloned gene as an esterase. via d ...19938254303
ciprofloxacin as prophylaxis for urinary tract infection: prospective, randomized, cross-over, placebo controlled study in patients with spinal cord lesion.to evaluate the efficacy of low-dose long-term treatment with ciprofloxacin in the prevention of recurrent urinary tract infections in patients with spinal cord lesions and neurogenic bladder dysfunction, a prospective, randomized, cross-over, placebo-controlled study was performed. the study period was 12 months long, including 6 months of treatment with 100 mg. ciprofloxacin at night and 6 months of placebo treatment. the study was completed by 18 men and 3 women, median age 38 years (range 19 ...19948254783
the antimicrobial activity in vitro of cefpirome and 6 other beta-lactam antibacterial agents against clinical isolates.the in vitro activity of cefpirome (cpr) was compared with that of cefazolin (cez), cefotiam (ctm), flomoxef (fmox), cefotaxime (ctx), ceftazidime (caz) and imipenem/cilastatin (ipm/cs) against 384 clinical isolates, for tested by the both microdilution method. the activity of cpr against staphylococcus aureus (mic50, 1 microgram/ml) and enterococcus faecalis (mic50, 8 micrograms/ml) was better than ctx and caz, but slightly less than ipm/cs. cpr and other antibacterial agents were not effect ag ...19938254889
characterization of acinetobacter haemolyticus aac(6')-ig gene encoding an aminoglycoside 6'-n-acetyltransferase which modifies amikacin.the amikacin resistance gene acc(6')-ig of acinetobacter haemolyticus bm2685 encoding an aminoglycoside 6'-n-acetyltransferase was characterized. the gene was identified as a coding sequence of 438 bp corresponding to a protein with a calculated mass of 16,522 da. analysis of the deduced amino acid sequence suggested that it was the fourth member of a subfamily of aminoglycoside 6'-n-acetyltransferases. the resistance gene was not transferable either by conjugation to escherichia coli or to acin ...19938257129
acinetobacter meningitis.10,468 csf samples from cases of meningitis in different age groups were cultured during 1988-1991. acinetobacter calcoaceticus was isolated in 12 (5.6%) of 211 positive cultures. the strain were 100% resistant to ampicillin, cotrimoxazole and tetracycline 50% resistant to cephazolin gentamicin and kanamycin but 100% susceptible to chloramphenicol.19938258473
vascular catheter-related bloodstream infection due to acinetobacter johnsonii (formerly acinetobacter calcoaceticus var. lwoffi): report of 13 cases.although acinetobacter baumannii (formerly acinetobacter calcoaceticus var. anitratus) is known to be an important nosocomial pathogen, the clinical impact of other acinetobacter species is not fully understood. acinetobacter johnsonii (formerly a subset of a. calcoaceticus var. lwoffii) is considered a commensal on human skin, and infections due to this organism have not been reported previously. during a study period of 18 months, however, 13 patients at 6 hospitals developed catheter-related ...19938268343
acinetobacter in denmark: ii. molecular studies of the acinetobacter calcoaceticus-acinetobacter baumannii complex.the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four closely related "genospecies" or dna groups: dna group 1 (a. calcoaceticus), dna group 2 (a. baumannii), dna 3, and tjernberg & ursing's dna group 13. strains in this complex are so similar phenotypically that it is often impossible to identify them to the dna group level by the use of biochemical tests. twenty-three danish clinical strains from 23 patients phenotypically identified to the a. calcoaceticus-a. bauman ...19938286091
molecular characterization of an aberrant mercury resistance transposable element from an environmental acinetobacter strain.we present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pklh2 from an environmental bacterium, acinetobacter calcoaceticus, isolated from a mercury mine. the pklh2 mer operon has essentially the same gene organization as that of tn21 and tn501 from clinical bacteria. the pklh2 mer operon nucleotide sequence shows 85.5% identity with the tn501 and 80.9% identity with the tn21 sequences. vestigial sequences have been found at the ends of the pklh2 mer ope ...19938302940
[bacteriologic examinations of patients with burns].bacteriological studies were performed on 189 patients treated during 1985-1989. 800 samples were tested which were taken from various sites of burn wound (3-5 smears from a patient). surface of burn amounted to 15-86% and degree of burn was iib/iii. in 772 samples, 2073 bacterial strains were found. most frequent were pseudomonas aeruginosa (31%) and staphylococcus aureus (29%). remaining strains consisted chiefly of proteus mirabilis, pseudomonas cepacia, klebsiella pneumoniae, escherichia col ...19938309304
identification of the transcriptional activator pobr and characterization of its role in the expression of poba, the structural gene for p-hydroxybenzoate hydroxylase in acinetobacter calcoaceticus.we have identified pobr, a gene encoding a transcriptional activator that regulates expression of poba, the structural gene for p-hydroxybenzoate hydroxylase (poba) in acinetobacter calcoaceticus adp1. inducible expression of cloned poba in escherichia coli depended upon the presence of a functional pobr gene, and mutations within pobr prevented poba expression in a. calcoaceticus. a poba-lacz operon fusion was used to demonstrate that poba expression in a. calcoaceticus is enhanced up to 400-fo ...19938331077
synthesis of allyl o-[sodium(alpha-d-glycero-d-talo-2- octulopyranosyl)onate]-(2-->6)-2-acetamido-2-deoxy-beta-d-glucopyranosi de, a core constituent of the lipopolysaccharide from acinetobacter calcoaceticus nctc 10305.reaction of methyl 2,6-anhydro-2,3-dideoxy-d-manno-2-octenoate 1 with 3-chloroperoxybenzoic acid gave the 2,3-anhydro derivative 2, which was converted into the per-o-acetylated anomeric methyl glycosides of d-glycero-d-galacto-2-octulopyranosylonic acid in good yield. subsequent inversion of the configuration at c-3 and deprotection afforded sodium (methyl beta-d-glycero-d-talo-2-octulopyranosid)onate. alternatively, 2 was transformed into methyl (alpha-d-glycero-d-talo-2- octulopyranosyl bromi ...19938339301
microbial purification technique of mineral dressing plants reject waters.earlier investigations on laboratory and pilot plant scale have shown the resort to microorganisms to be a practicable approach to the problem of purifying mineral dressing plant reject waters from residual flotation reagents and/or metal ions. in spite of the proven effectiveness of this method, one major drawback, namely the pathogenicity of some microorganisms, has so far hampered its application on a commercial scale. a research programme, aimed at developing a microbial reject water purific ...19938357615
isolation of acinetobacter calcoaceticus from the gastrointestinal tracts of scid mice.acinetobacter calcoaceticus colonization was observed in the gastrointestinal tracts of c.-b17-scid/scid (scid) mice, while it was not observed in c.b17-scid/+ and c.b17-+/+ mice with normal immunity housed under the same conditions. a. calcoaceticus and other viable enteric bacteria were not isolated from any organs other than gastrointestinal tract in scid mice. the mice colonized with this organism were apparently healthy and no significant visceral lesion was observed.19938366666
resistance to third-generation cephalosporins in barbados.resistance to third-generation, or extended-spectrum, cephalosporins caused by induction of class i beta-lactamases has been reported rarely from developing countries. seven isolates of cephalosporin-resistant gram-negative rods were recovered recently from urine, burns and ulcers in the queen elizabeth hospital, barbados. the isolates were identified as acinetobacter calcoaceticus (2), citrobacter freundii (1), enterobacter cloacae (1), morganella morganii (1), providencia stuartii (1) and prot ...19938367967
evaluation of a commercial dna purification system for plasmid analysis of nosocomial bacterial pathogens.this study evaluated the promega magic minipreps (mm) (promega corporation, madison, wi) dna purification system for use in plasmid analysis of common nosocomial bacterial pathogens. the mm system is a kit that includes lysis solutions and buffers and incorporates a minicolumn of dna binding resin for recovery of plasmid dna. the mm system was used according to the manufacturer's directions to recover plasmids for agarose gel electrophoresis from clinical isolates of acinetobacter calcoaceticus, ...19938379541
transposon mutagenesis in acinetobacter calcoaceticus rag-1.molecular genetic studies of acinetobacter spp. have been greatly limited by the lack of a method for transposon mutagenesis. in this study, a genetically engineered derivative of tn10, mini-tn10pttkm, was conjugally transferred in plate matings from escherichia coli sm10[(lambda pir)(plofpttkm)] to acinetobacter calcoaceticus rag-1. transfer frequencies were dependent on mating ratios and varied from 7.9 x 10(-8) to 3.4 x 10(-7) per recipient cell. the 27 lipase-deficient transconjugants which ...19938383667
[community-acquired meningitis caused by acinetobacter calcoaceticus var. lwoffi in a healthy host]. 19938399482
crystallization and preliminary crystallographic investigations of the soluble glucose dehydrogenase from acinetobacter calcoaceticus.single crystals of the soluble glucose dehydrogenase (gdh) from acinetobacter calcoaceticus have been grown by the vapour diffusion method. these crystals diffract to beyond 2.1 a and are suitable for x-ray crystallography. the space group was determined to be p2(1) with unit cell parameters a = 55.5 a, b = 104.5 a, c = 86.5 a and beta = 99.8 degrees. one asymmetric unit contains a dimer of the gdh molecule.19938411180
effect of sulbactam on infections caused by imipenem-resistant acinetobacter calcoaceticus biotype anitratus.a recent outbreak of multiresistant strains of acinetobacter calcoaceticus biotype anitratus was observed mostly, but not exclusively, in the surgical intensive care unit in our hospital. disk diffusion and microdilution susceptibility studies demonstrated resistance to imipenem, all aminoglycosides, and all individual beta-lactam antibiotics. only ampicillin plus sulbactam, cefoperazone plus sulbactam, and polymyxin produced zone sizes and mics in the susceptible ranges. determination of mics a ...19938421178
physiological characterization of natural transformation in acinetobacter calcoaceticus.acinetobacter calcoaceticus bd413 develops competence for natural transformation immediately after the start of the exponential growth-phase and remains competent up to e few hours into the stationary phase, after which competence gradually declines. the transformation frequencies obtained strongly depend on the kind of transforming dna and the incubation time with dna. up to 25% of the cells in a culture can be transformed. dna uptake in acinetobacter does not display sequence specificity, is m ...19938436948
microbial contamination of enteral feeding solution and its prevention.in an investigation of microbial contamination of enteral feeding solutions, all 22 residual solutions obtained immediately after administration were contaminated at concentrations of 10(3) to 10(6) viable counts/ml. major contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas aeruginosa and acinetobacter calcoaceticus var anitratus. contamination seemed to have been caused by frequent reuse of bag-type containers and the infusion tubes connected to the bags, neither o ...19938442520
evolutionary divergence of poba, the structural gene encoding p-hydroxybenzoate hydroxylase in an acinetobacter calcoaceticus strain well-suited for genetic analysis.the poba gene encoding p-hydroxybenzoate hydroxylase (poba) from acinetobacter calcoaceticus has been developed as a genetic tool for the analysis of structure-function relationships in this enzyme. by exploiting the favorable genetic system of a. calcoaceticus strain adp1, it is possible both to select and to map mutations which disturb poba activity; characterization and sequence determination of mutants derived in this manner may complement site-directed studies with the homologous pseudomona ...19938449410
precise deletions in large bacterial genomes by vector-mediated excision (vex). the trfa gene of promiscuous plasmid rk2 is essential for replication in several gram-negative hosts.we have developed a simple and efficient method of vector-mediated excision (vex) for in vivo generation of precisely defined deletions in large bacterial genomes. the system uses homologous recombination with small cloned fragments on specialized pvex plasmids to insert directly repeated bacteriophage p1 loxp sites at positions flanking the region to be deleted. in the presence of cre recombinase, the loxp sites are efficiently recombined to produce the deletion. deletion endpoints can be direc ...19938450534
acinetobacter, an infrequent cause of community acquired bacterial meningitis.a 19-year-old previously healthy woman developed meningitis due to acinetobacter calcoaceticus. this is a gram-negative coccobacillus belonging to the family neisseriaceae. most reported cases of acinetobacter meningitis were hospital-acquired and due to strains highly resistant to amoxycillin. our patient was treated with amoxycillin and recovered without sequelae.19938453820
comparison of the nucleotide sequences of the meta-cleavage pathway genes of tol plasmid pww0 from pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution.tol plasmid pww0 from pseudomonas putida mt-2 encodes catabolic enzymes required for the oxidation of toluene and xylenes. the structural genes for these catabolic enzymes are clustered into two operons, the xylcmabn operon, which encodes a set of enzymes required for the transformation of toluene/xylenes to benzoate/toluates, and the xylxyzltegfjqkih operon, which encodes a set of enzymes required for the transformation of benzoate/toluates to krebs cycle intermediates. the latter operon can be ...19938510667
antibacterial properties of am-1155, a new 8-methoxy quinolone.am-1155 is a new 8-methoxy quinolonecarboxylic acid with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of methicillin-susceptible staphylococcus aureus, streptococci, enterococcus faecalis, most of the enterobacteriaceae, acinetobacter calcoaceticus and haemophilus influenzae at the concentration of 0.39 mg/l. am-1155 was 2- to 16-fold more active than ciprofloxacin against gram-positive organisms including methicillin-resistant staphylococci. the an ...19958522459
tn5-directed cloning of pqq genes from pseudomonas fluorescens cha0: mutational inactivation of the genes results in overproduction of the antibiotic pyoluteorin.pseudomonas fluorescens cha0 produces several secondary metabolites, e.g., the antibiotics pyoluteorin (plt) and 2,4-diacetylphloroglucinol (phl), which are important for the suppression of root diseases caused by soil-borne fungal pathogens. a tn5 insertion mutant of strain cha0, cha625, does not produce phl, shows enhanced plt production on malt agar, and has lost part of the ability to suppress black root rot in tobacco plants and take-all in wheat. we used a rapid, two-step cloning-out proce ...19958526497
tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria.a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ...19958554762
acinetobacter endocarditis in children: a case report and review of the literature.acinetobacter calcoaceticus, a gram-negative bacterium ubiquitous in soil, water and sewage, is a rare cause of endocarditis in children. the first case of acinetobacter endocarditis in an infant is described. this patient had underlying tetralogy of fallot with absent pulmonary valve. a review of the literature in english revealed only four other cases of acinetobacter endocarditis in children; three of whom had underlying congenital heart disease. like the other reported cases, this patient re ...19958557392
phenol degradation by acinetobacter calcoaceticus ncib 8250.acinetobacter calcoaceticus ncib 8250 utilizes phenol as sole source of carbon and energy via an ortho-cleavage pathway. the presence of ethanol in mixed substrate cultivations repressed the utilization of phenol. in fed batch cultivation the phenol tolerance was increased at least 2-fold. maximum degradation rates of 150 mg phenol/(1 h) and 280 mg phenol/(g h), respectively were observed. phenol hydroxylase is induced by its substrate and in parallel the catechol-1,2-dioxygenase is detectable. ...19958568644
differentiation of acinetobacter calcoaceticus sensu stricto from related acinetobacter species by electrophoretic polymorphism of malate dehydrogenase, glutamate dehydrogenase and catalase.acinetobacter baumannii, unnamed acinetobacter species 3 (studied by p.j.m. bouvet and p.a.d. grimont) and unnamed dna group 13 (studied by i. tjernberg and j. ursing) are the most prevalent acinetobacter species in hospitals. using the identification scheme of bouvet and grimont, it is sometimes difficult to differentiate these species from a. calcoaceticus sensu stricto, a species of the natural environment that has seldom been found associated with human infection. genetically identified acin ...19958584799
the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species.the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ...19958587109
study of a hydrocarbon-utilizing and emulsifier-producing acinetobacter calcoaceticus strain isolated from heating oil.twenty bacterial strains were isolated from a sample of contaminated heating oil and screened for their ability to use petroleum and several common fuels as the sole source of carbon and energy. one of the isolates, named mm5, was able to grow on petroleum derivatives and brought about an emulsification of those compounds. gas chromatography studies showed that strain mm5 was able to degrade hydrocarbons of heating oil. mm5 has been tentatively identified as a strain of acinetobacter calcoacetic ...19958588840
infection of the pancreatic duct following pancreas transplantation with bladder drainage.combined kidney-pancreas transplantation represents a widely accepted therapy for endstage diabetic nephropathy. drainage of the pancreatic juice into the urinary bladder is the preferred surgical technique in most centers. between january 1987 and december 1994 a total of 85 pancreas transplantations with pancreatocystostomy were performed at the innsbruck university hospital. in all cases a polyvinylcatheter was placed into the pancreatic duct and drained transvesically to the exterior. during ...19958596128
genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in acinetobacter calcoaceticus ncib8250.we have mutated acinetobacter calcoaceticus ncib-8250 to growth deficiency on phenol as sole carbon source and isolated genes with similarity to phenol hydroxylase and catechol 1,2-dioxygenase by complementation. sequence analysis reveals the presence of six open reading frames (orfs) with similarities to a pseudomonas multicomponent phenol hydroxylase which are followed by an orf with similarity to cata from a. calcoaceticus adp1. transformation of these genes to adp1 confers the ability to gro ...19958596453
a comparative study of different pcr-based dna fingerprinting techniques for typing of the acinetobacter calcoaceticus-a. baumannii complex.different pcr-based dna fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the acinetobacter calcoaceticus-a. baumannii complex. seven isolates belonged to a previously defined outbreak while 19 isolates were unrelated epidemiologically. the pcr-based dna fingerprinting techniques used were: (i) repetitive extragenic palindromic (rep) pcr; (ii) enterobacterial repetitive intergenic consensus (eric) pcr; (iii) randomly amplified polymorphic dna with m13 forward ...19968636966
antibiotic resistance of acinetobacter calcoaceticus strains isolated from patients treated in intensive care units.the distribution according to specimens and susceptibility to antimicrobial agents of 481 acinetobacter calcoaceticus strains isolated from patients treated in intensive care units were studied. they occurred most frequently in tracheal specimens and pus. using disk diffusion test the strains proved to be multiple resistant to ampicillin (86.3%), azlocillin (86.7%), mezlocillin (84.0%), cefamandole (99.7%), cefoxitin (94.1%), cefuroxime (90.6%), cefoperazone (84.9%), cefotaxime (82.0%), ceftriax ...19958689090
cloning and expression of dipeptidase from acinetobacter calcoaceticus atcc 23055.the gene encoding dipeptidase was cloned from acinetobacter calcoaceticus atc 23055. determination of the nucleotide sequence revealed that the gene had an open reading flame of 1,050 bp coding a protein of 350 amino acids. the deduced amino acid sequence showed 48.8% similarity to human renal dipeptidases and conserved two amino acid residues identified in human and pig renal dipeptidases as essential ones for the catalytic activity. purified recombinant enzyme expressed in escherichia coli did ...19958690717
cloning of catbcijfd genes for catechol degradation into chromosomal poba and genetic stability of the recombinant acinetobacter calcoaceticus.a possible obstacle in the development of hybrid strains of acinetobacter calcoaceticus by the introduction of a metabolic pathway into the chromosome is genetic instability of the resulting recombinant strains. therefore, the possibility that the poba gene can be used as a chromosomal cloning site where the transposed genes can be maintained and expressed, was explored in this study. for this purpose, two model hybrid strains of a. calcoaceticus were created, in which a dna fragment carrying ca ...19968695911
natural transformation in river epilithon.natural transformation was demonstrated in unenclosed experiments incubated in river epilithon. strains of acinetobacter calcoaceticus were transformed to prototrophy by either free dna (lysates) or live donor cells. the sources of transforming dna and recipient culture were immobilized on filters, secured to stones, and incubated midstream in the river. the transfer frequency generally increased with temperature. no transfer was detected in the river taff below 10 degrees c. the age of the reci ...19968702292
in vitro activity of the tricyclic beta-lactam gv104326.gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...19968723475
validation of use of whole-cell repetitive extragenic palindromic sequence-based pcr (rep-pcr) for typing strains belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak.acinetobacter spp. are being reported with increasing frequency as causes of nosocomial infection. in order to identify reservoirs of infection as quickly as possible, a rapid typing method that can differentiate epidemic strains from environmental and nonepidemic strains is needed. in 1993, a cluster of acinetobacter baumannii isolates from five patients in the adult intensive therapy unit of our tertiary-care teaching hospital led us to develop and optimize a rapid repetitive extragenic palind ...19968727902
microbial colonization of large wounds.this study determines the nature of microbial wound colonization in 28 patients with large burns admitted to the burn centre, haukeland university hospital, bergen. altogether, 748 swabs were taken in 141 sampling procedures. a total of 414 microbial isolates were detected and their resistance patterns to a variety of systemic antimicrobial agents determined. the most frequent isolates were coagulase-negative staphylococci (21.5 per cent) and staphylococcus aureus (14 per cent), followed by ente ...19958747728
[identification and typing of hospital strains of acinetobacter calcoaceticus-acinetobacter baumanni complex].a collection of 95 strains of the acinetobacter calcoaceticus-acinetobacter baumannii complex, isolated between 1991 and 1993 in the prague burn center (bc), was studied. ninety-one strains were isolated from 43 patients: 50 of them from burnt sites, 22 from endotracheal tube, 13 from urine, 3 from blood and 3 from venous catheter, and 4 strains were isolated from the hospital environment and the nursing staff. the strains were classified by restriction endonuclease fingerprinting of total dna, ...19968756404
is1236, a newly discovered member of the is3 family, exhibits varied patterns of insertion into the acinetobacter calcoaceticus chromosome.analysis of spontaneous mutations in acinetobacter calcoaceticus revealed a 1237 bp insertion sequence named is1236 and possessing a nucleotide sequence resembling those of members of the ls3 family. the chromosome of a. calcoaceticus strain adp1 contains seven copies of is1236 which appears to insert preferentially into pobr, the transcriptional activator of the structural gene for p-hydroxybenzoate hydroxylase. is1236 creates tandem 3 bp dna duplications flanking the sites of its insertion in ...19968757745
a reappraisal of the diversity and class distribution of aspartate transcarbamoylases in gram-negative bacteria.recently, the subunit composition of class a aspartate transcarbamoylases (atcases) in fluorescent pseudomonads has been clarified. we present evidence that distribution of this type of atcase may be more widespread than at first suspected. bacterial atcases exist in three forms: class a (molecular mass approximately 450-500 kda); class b, typified by escherichia coli atcase (approximately 300 kda); and class c, typified by bacillus subtilis atcase (approximately 100 kda). using gradient gel ele ...19968757751
evaluation of the ability of a commercial system to identify acinetobacter genomic species.a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ...19968781881
[in vitro antibacterial activity of a new parenteral penem, sulopenem].eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ...19968786624
[in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic].the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ...19968786626
changing trends in the antimicrobial susceptibility of clinical isolates of acinetobacter baumannii.acinetobacter baumannii has recently emerged as an important nosocomial pathogen because of its resistance to the majority of commonly used antimicrobial agents. therefore, the pattern of isolation of acinetobacter baumannii in our laboratory and the in vitro susceptibilities to various antimicrobial agents were studied. one hundred and two clinical isolates of previously identified acinetobacter calcoaceticus var. anitratus from different patients were collected from the diagnostic bacteriology ...19968799001
ptim3, a plasmid delivery vector for a transposon-based inducible marker gene system in gram-negative bacteria.ptim3 is a suicide plasmid vector for the delivery of a transposition defective derivative of tn5, expressing inducible mercury resistance (hgr) and catechol 2,3-dioxygenase (c23o) activity, to a range of gram-negative microorganisms. ptim3 was constructed by a four-stage process from pnmm1, a derivative of psup5011 containing a modified tn5 where antibiotic-resistance determinants have been replaced by the mer operon of tn501 and tdnc (encoding a c23o). in ptim3, tdnc is fused to merd, to bring ...19958825369
physiological factors affecting production of extracellular lipase (lipa) in acinetobacter calcoaceticus bd413: fatty acid repression of lipa expression and degradation of lipa.the extracellular lipase (lipa) produced by acinetobacter calcoaceticus bd413 is required for growth of the organism on triolein, since mutant strains that lack an active lipase fail to grow with triolein as the sole carbon source. surprisingly, extracellular lipase activity and expression of the structural lipase gene (lipa), the latter measured through lacz as a transcriptional reporter, are extremely low in triolein cultures of lipa+ strains. the explanation for this interesting paradox lies ...19968830702
emergence of resistant isolates of acinetobacter calcoaceticus- a. baumannii complex in a spanish hospital over a five-year period.acinetobacter calcoaceticus-a. baumannii complex species have emerged as a relevant cause of nosocomial infection and colonization over the past 20 years, mainly in intensive care units. the aim of this study was to investigate the in vitro activity of 14 antimicrobial agents against 177 clinical isolates from patients admitted to a spanish teaching hospital over a five-year period. susceptibility rates or 99%, 99%, and 74% were obtained for imipenem, meropenem, ampicillin plus sulbactam, and am ...19968839649
in vitro and in vivo activities of lb10522, a new catecholic cephalosporin.in vitro activity of lb10522 was compared with those of cefpirome, ceftazidime, ceftriaxone, and cefoperazone against clinical isolates. against gram-positive bacteria, lb10522 was most active among the compounds tested. it was fourfold more active than cefpirome against methicillin-susceptible staphylococcus aureus and enterococcus faecalis. lb10522 was highly effective against most members of the family enterobacteriaceae tested. ninety percent of isolates of escherichia coli, klebsiella oxyto ...19968843288
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