TitleAbstractYear(sorted ascending)
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates].this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ...19902287060
dna sequences of genes encoding acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of dna sequences within genes during their evolutionary divergence.the dna sequence of a 2,391-base-pair hindiii restriction fragment of acinetobacter calcoaceticus dna containing the pcachg genes is reported. the dna sequence reveals that a. calcoaceticus pca genes, encoding enzymes required for protocatechuate metabolism, are arranged in a single transcriptional unit, pcaefdbchg, whereas homologous genes are arranged differently in pseudomonas putida. the pcag and pcah genes represent separate reading frames respectively encoding the alpha and beta subunits o ...19902298704
an evolutionary comparison of acinetobacter calcoaceticus trpf with trpf genes of several organisms.the deduced amino acid sequence of acinetobacter calcoaceticus n-(5'-phosphoribosyl) anthranilate isomerase (prai), which is coded by trpf, was compared with trpf of caulobacter crescentus, escherichia coli, bacillus subtilis, saccharomyces cerevisiae, neurospora crassa, and aspergillus nidulans. sixty percent of identical or similar amino acids were located in alpha/beta tim (triose-phosphate isomerase) barrels and in residues important in substrate binding and catalysis. in addition, the analy ...19902299982
the in-vitro activity of ceftibuten against 475 clinical isolates of gram-negative bacilli, compared with cefuroxime and cefadroxil.the in-vitro activity of ceftibuten was compared with cefuroxime and cefadroxil against 475 clinically-significant, epidemiologically-distinct isolates of gram-negative bacilli: 170 from blood, 212 from urine and 93 from a supplementary collection of multiply-resistant strains known to have resistance plasmids, to have caused sporadic or epidemic nosocomial infection, or both. ceftibuten mics ranged from 0.003 to greater than 32 mg/l, with a modal mic of 0.01 mg/l: 95% of all isolates had ceftib ...19902329100
[studies on respiratory infections in primary care clinic (ii). distribution and antibiotic sensitivity to 45 agents of bacteria isolated from patients with respiratory infections visiting a doctor in private practice].the bacteriology of the isolates from the sputum or the throat swab of patients with respiratory infections visiting a doctor in private practice in sendai city during the period from march in 1988 to february in 1989 was documented, and their sensitivity to 45 antimicrobial agents was determined. of the 568 patients, 514 cases had acute pharyngitis, 8 cases each had acute tonsillitis and acute bronchitis, 7 cases were acute pneumonia, 6 cases had herpangina, 18 cases had hand-foot-mouth disease ...19902335751
identification and nucleotide sequence of the acinetobacter calcoaceticus encoded trpe gene.the trpe gene from acinetobacter calcoaceticus encoding the anthranilate synthase component i was cloned, identified by deletion analysis and sequenced. it encodes a predicted polypeptide of 497 amino acids with a calculated molecular weight of 55,323. its primary structure shows 49% identical amino acids with the enzyme from clostridium thermocellum, 45% with that of thermus thermophilus and only 35% with that of escherichia coli. the codon usage of the trpe genes encoding the most homologous e ...19902338941
[evaluation of imipenem/cilastatin sodium in the treatment of respiratory tract infections].imipenem/cilastatin sodium (ipm/cs) was administered to 55 patients with respiratory tract infections (rti). a clinical evaluation of ipm/cs was carried out in 51 patients, 28 with pneumonia, 4 with pulmonary abscess, 1 with pyothorax, 6 with bronchitis, 9 with bronchiectasis, 1 with diffuse panbronchiolitis and 2 with rti with chronic obstructive pulmonary disease, and the clinical efficacy rate was 78.4%. causative organisms were isolated in 23 strains out of 20 patients, such as staphylococcu ...19902348550
concerning the sensitivity of acinetobacter calcoaceticus.the strains of acinetobacter calcoaceticus, var. anitratus (a. c. a.) were isolated in the nosocomial environment as an opportune pathogen. the therapy of choice may be determined after in vitro tests. our results show following therapeutical possibilities: beta-lactam antibiotics--cephalosporins of iiird generation (cefotaxime), also combinations of antimicrobials have shown good results: amoxycillin or ticarcillin with clavulanic acid. best synergistic effect was found in combination ticarcill ...19902351822
airborne microbial contents in two types of swine confinement buildings in quebec.airborne microorganisms were isolated with a sampler in two types of swine confinement buildings (farrowing units and fattening units). respirable (particles less than 5 microns) and total dust fractions were obtained. samplings were repeated every 2 weeks for a total of 6 samplings per unit between january and april. the predominant microorganisms isolated were bacteria (up to 1.25 x 10(6) cfu/m3) with an important fraction in the respirable size range (up to 0.5 x 10(6) cfu/m3). only small qua ...19902353639
in vitro studies of ciprofloxacin and survey of resistance patterns in current isolates.we studied the activity of ciprofloxacin and other antibiotics against both routine and multiresistant (multi-r) clinical isolates. ciprofloxacin inhibited more than 98% of most species of enterobacteriaceae at a concentration of 2 micrograms/ml. only acinetobacter calcoaceticus, and to a much lesser degree, providencia and serratia, were resistant. most pseudomonas aeruginosa isolates were susceptible. only 1% of staphylococci were resistant; the test panel included 1200 mrsa. for most species ...19902369816
oral pefloxacin mesylate in the treatment of continuous ambulatory peritoneal dialysis associated peritonitis: an open non-comparative study.twenty-two (15 female and 7 male) patients with continuous ambulatory peritoneal dialysis (capd) associated peritonitis were entered into an open non-comparative evaluation of oral pefloxacin mesylate. an initial loading dose of 800 mg was followed by 400 mg twice daily. five patients were subsequently excluded or withdrawn. the mean age of the patients was 44 +/- 12.6 years. all three gram-negative infections (escherichia coli, acinetobacter calcoaceticus, serratia liquefaciens) and all three c ...19902373667
investigation of an outbreak of acinetobacter calcoaceticus var. anitratus infections in an adult intensive care unit. 19902403220
biotransformation of alkyl and aryl carbonates. microbial enriched mixed culture was successfully grown on model alkyl and aryl carbonates. these compounds were degraded by microorganisms at different rates. p-chlorophenyl-2-octyl carbonate and p-nitrobenzyl-2-octyl carbonate were metabolized through the formation of p-chlorophenol and p-nitrobenzyl alcohol respectively. a strain of acinetobacter calcoaceticus isolated from the mixed culture utilized phenyl-2-octyl carbonate by an intracellular hydrolase to phenol and 2-octanol which were further me ...19901366389
natural transformation in acinetobacter calcoaceticus.acinetobacter calcoaceticus is a metabolically versatile microorganism that is naturally competent for dna uptake and incorporation. we have exploited the natural state of competency for studies involving the cloning, organization and expression of genes encoding catabolic enzymes. a. calcoaceticus is able to take up, at high efficiency, genetically engineered dna, incorporate the dna and stably maintain and express the dna. sequence analysis of cloned a. calcoaceticus dna reveals a great deal o ...19901366417
carbohydrate utilization in streptococcus thermophilus: characterization of the genes for aldose 1-epimerase (mutarotase) and udpglucose 4-epimerase.the complete nucleotide sequences of the genes encoding aldose 1-epimerase (mutarotase) (galm) and udpglucose 4-epimerase (gale) and flanking regions of streptococcus thermophilus have been determined. both genes are located immediately upstream of the s. thermophilus lac operon. to facilitate the isolation of gale, a special polymerase chain reaction-based technique was used to amplify the region upstream of galm prior to cloning. the galm protein was homologous to the mutarotase of acinetobact ...19901694527
[gram-negative flora of horticulture products prevalently to be consumed fresh. ii, acinetobacter calcoaceticus]. 19901711345
in-vitro and in-vivo activities of sulbactam and ytr830h against acinetobacter calcoaceticus.the antibacterial activities of sulbactam and ytr830h, both beta-lactamase inhibitors, were studied against acinetobacter calcoaceticus, in vitro and in vivo. in-vitro susceptibility tests showed both to be very active against 80 clinical isolates of a. calcoaceticus. against an intraperitioneal infection in mice, caused by a. calcoaceticus ac54, the 50% effective doses of sulbactam and ytr830h were 1.62 and 16.7 mg/kg, respectively. against a. calcoaceticus s31, a strain resistant to gentamicin ...19901964157
the survival of acinetobacter calcoaceticus inoculated on fingertips and on formica.when inoculated on the fingers of three volunteers, strains of acinetobacter calcoaceticus var. anitratus survived better than strains of var. lwoffii; 60 min after an inoculum of 10(4) cfu/finger, washings yielded between 2.6 x 10(1) (for a sporadic strain of var. lwoffii) and 7.2 x 10(2) cfu/finger (for an epidemic strain of var. anitratus). all five test strains survived better on formica than on skin, and from an inoculum of 10(4) cfu, between 6.4 x 10(2) and 2.2 x 10(3) cfu/finger were reco ...19901971628
[a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 3. inhibition of the enzyme].the alanine aminopeptidase from acinetobacter calcoaceticus is inhibited by sh-reagents like p-hydroxymercuribenzote, ellman's reagent, n-bromosuccinimide, and metal chelating agents like 1,10-phenanthroline. the aap is competitively inhibited by l-amino acids such as leucine, phenylalanine, and valine having hydrophobic side chains. bacitracin (ki = 2.0.10(-6) mol/l) inhibits aap stronger than puromycin (ki = 8.0.10(-6) mol/l). in contrast, the aeromonas aminopeptidase (ec is stronge ...19901980190
[epidemiologic study of pathogens causing nosocomial infections].nosocomial infection is a global problem. it significantly adds to the expected duration of hospitalization resulting with an increased economic burden and represents a leading cause of death. the characteristics of nosocomial infection vary from country to country and also changes with time. in order to understand the pathogens causing nosocomial infection in the large teaching hospital in taiwan, we collected and analysed the surveillence data of nosocomial infection at the national taiwan uni ...19901982123
mechanisms for biopolymer accumulation in immobilized acinetobacter calcoaceticus system.the gram-positive bacteria, acinetobacter calcoaceticus, is capable of accumulating biopolymer in the carrier matrix of an immobilized cell system. several possible mechanisms for the biopolymer accumulation are evaluated. it appears that direct solid surface polymer adsorption and polymer diffusion limitation within the pore space are minor factors in biopolymer accumulation. calculations demonstrate that the cell bound polymer to dry cell weight ratio is much higher for immobilized cells than ...199018595094
effect of cetylpyridinium chloride on microbial adhesion to hexadecane and polystyrene.microbial adhesion at the oil-water interface is a subject of both basic interest (e.g., as a technique for the measurement of hydrophobicity) and applied interest (e.g., for use in two-phase oil-water mouthwashes for the desorption of oral microorganisms). in general, surfactants inhibit microbial adhesion to oils and other hydrophobic surfaces. in the present study, we demonstrated that the cationic surfactant cetylpyridinium chloride (cpc) significantly enhanced microbial adhesion to hexadeca ...199016348211
diagnosis of cellulitis in the immunocompromised host.a prospective study of diagnostic techniques in cellulitis was performed on 28 patients with malignancy. twenty-two (78%) of the fine needle aspiration cultures and 10 (35%) of the blood cultures were positive in this immunocompromised population. the incidence of positive fine needle aspiration cultures (p<0.005) or bacteremia (p<0.0005) was significantly higher than results obtained in an immunocompetent population with cellulitis at the same institution. staphylococci or streptococci were rec ...199022553455
self-cycling fermentation applied to acinetobacter calcoaceticus rag-1.the self-cycling fermentation (scf) technique was applied to a culture of acinetobacter calcoaceticus rag-1. this method was shown to result in synchronization of the cells, achieving a 77% improvement in cell synchrony over that of the batch case. cellular occurrences, averaged out by asynchronous batch cultures, were magnified by the temporal alignment of metabolic events brought about by the synchronization associated with scfs. the cell population doubled only once per cycle, thus establishi ...199116348564
2,2'-oxo-1,1'-azobenzene: microbial transformation of rye (secale cereale l.) allelochemical in field soils byacinetobacter calcoaceticus: iii.acinetobacter calcoaceticus, a gram-negative bacterium isolated from field soil, was found to be responsible for the biotransformation of 2(3h)-benzoxazolinone (boa) to 2,2'-oxo-1,1'-azobenzene (azob). experiments were conducted to evaluate the transformation of boa to azob by this microbe in sterile and nonsterile soil. transformation studies with soils inoculated witha. calcoaceticus indicated that the production of azob increased linearly with the concentration of boa in sterile soil and show ...199124257881
comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in acinetobacter calcoaceticus and from the tol-plasmid-encoded toluene pathway in pseudomonas putida. n-terminal amino acid sequences, amino acid compositions and immunological cross-reactions.1. n-terminal sequences were determined for benzyl alcohol dehydrogenase, benzaldehyde dehydrogenase i and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus n.c.i.b. 8250, benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the tol plasmid pww53 in pseudomonas putida mt53 and yeast k(+)-activated aldehyde dehydrogenase. comprehensive details of the sequence determinations have been deposited as supplementary publication sup 50161 (5 pages) at the british library d ...19911989592
immune response to acinetobacter calcoaceticus infection in man.after growth in an iron-depleted chemically-defined medium acinetobacter calcoaceticus expressed four high mol. wt outer-membrane proteins (omps) which were repressed under iron supplementation or in a complex laboratory medium. immunoblotting with serum from a septicaemic patient infected with a. calcoaceticus revealed antibody binding to these iron-repressible omps, indicating that they were expressed in vivo, and also to the 42- and 18-kda omps. although the antibody response to the omps did ...19911990144
fatal septicaemia with selenomonas sputigena and acinetobacter calcoaceticus. a case report.a 38-year-old man with a history of alcohol abuse developed rapidly fulminating septicaemia and died. selenomonas sputigena and acinetobacter calcoaceticus were isolated from a blood culture. selenomonas sputigena is a motile anaerobic gram-negative rod rarely associated with systemic disease. difficulties in isolation and taxonomic identification are discussed.19911993118
reliability of phenotypic tests for identification of acinetobacter species.a numerical approach was used for identification of 198 acinetobacter strains assigned to dna groups according to the classification of tjernberg and ursing (i. tjernberg and j. ursing, apmis 97:595-605, 1989). the matrix used was constructed from data published by bouvet and grimont (p.j.m. bouvet and p.a.d. grimont, int. j. syst. bacteriol. 36:228-240, 1986) and bouvet and jeanjean (p.j.m. bouvet and s. jeanjean, res. microbiol. 140:291-299, 1989). the tests chosen were those of the simplified ...19912007635
cellular fatty acid composition of acinetobacter strains.the cellular fatty acids of the different biotypes of acinetobacter calcoaceticus were determined by high resolution gas-liquid chromatography. while all strains exhibited, qualitatively, similar fatty acid compositions, quantitative differences were observed in strains belonging to defined phenotypic subgroups which can be used for differentiation.19912030649
nucleotide sequence analysis reveals linked n-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of streptomyces hygroscopicus.nucleotide sequence analysis of a 5,000-bp region of the bialaphos antibiotic production (bap) gene cluster defined five open reading frames (orfs) which predicted structural genes in the order bah, orf1, orf2, and orf3 followed by the regulatory gene, brpa (h. anzai, t. murakami, s. imai, a. satoh, k. nagaoka, and c.j. thompson, j. bacteriol. 169:3482-3488, 1987). the four structural genes were translationally coupled and apparently cotranscribed from an undefined promoter(s) under the positive ...19912066341
outer membrane permeability of acinetobacter calcoaceticus and its implication in antibiotic order to understand high and broad antibiotic resistance of acinetobacter calcoaceticus, the outer membrane permeability was studied. the permeability coefficients of zwitterionic cephalosporins in the intact cell outer membrane were 0.14-1.12 x 10(-4) cm3/min/mg protein. these values were two to seven times lower than the permeability coefficients of the same beta-lactams in the outer membrane of pseudomonas aeruginosa. the diffusion rates of carbapenems and zwitterionic cephalosporins into ...19911722802
isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of paracoccus denitrificans and characterization of the mutant strain.the periplasmically located cytochrome c553i of paracoccus denitrificans was purified from cells grown aerobically on choline as the carbon source. the purified protein was digested with trypsin to obtain several protein fragments. the n-terminal regions of these fragments were sequenced. on the basis of one of these sequences, a mix of 17-mer oligonucleotides was synthesized. by using this mix as a probe, the structural gene encoding cytochrome c553i (cycb) was isolated. the nucleotide sequence ...19911657873
conjugational transfer of recombinant dna in cultures and in soils: host range of pseudomonas putida tol plasmids.recombinant tol plasmid pwwo-eb62 allows pseudomonas putida to grow on p-ethylbenzoate. this plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rrna group i and escherichia coli. transfer of the recombinant plasmid to erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functi ...19911660698
acinetobacter calcoaceticus bacteremia: analysis of 48 cases.the medical records of 48 patients with acinetobacter calcoaceticus bacteremia over a recent two-year period were reviewed retrospectively to determine the epidemiologic and clinical features. there were 34 males and 14 females with a median age of 51 years. forty-six of the cases were hospital-acquired. the characteristics of these patients included residence in the intensive care unit, intubation and ventilatory support, preceding antibiotic therapy and intravascular catheterization. in vitro ...19911685177
survival of bacteria under dry conditions; from a viewpoint of nosocomial infection.the viability of non-sporing bacteria in a dry state was examined. the number of viable cells was determined at various time intervals, after inoculation onto cotton lint and a glass plate. viable cells of mycobacterium bovis were detected more than 2 months after inoculation, and this was the most resistant species to dry conditions among the bacteria tested. in the case of the gram-positive cocci tested and one species of gram-negative rods, acinetobacter calcoaceticus var. anitratus, the viab ...19911685507
[membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 4. amino acid chloromethyl ketones as ph-dependent, substrate-derived inhibitors of cysteine protease].the alanine aminopeptidase (aap) from a. calcoaceticus is a metal-ion-dependent cysteine protease bound to the inner membranes of the cells. amino acid chloromethyl ketones are highly specific inhibitors of the enzyme. the aap is inhibited at ph 7.5 (ph-optimum) by mixed-type inhibition. a competitive inhibition type results if the ph value is decreased to 6.2. in contrast, the inhibition of the aap by substrate-derived chloromethyl ketones is of the irreversible type at ph 8.25.19911686704
use of a broad-host-range gyra plasmid for genetic characterization of fluoroquinolone-resistant gram-negative bacteria.the gyra genotypes of ciprofloxacin-resistant clinical isolates of escherichia coli (n = 3), klebsiella pneumoniae (n = 4), providencia stuartii (n = 2), pseudomonas aeruginosa (n = 1), and acinetobacter calcoaceticus (n = 1) were analyzed in a dominance test. this test is based on the dominance of a wild-type gyra gene (gyra+) over the quinolone resistance allele (gyra) in a heterodiploid strain. plasmid pbp515, developed to carry the gyra+ gene of e. coli k-12 on a broad-host-range vector deri ...19911759823
[quantitation of acinetobacter calcoaceticus in mixed bacterial cultures by an enzyme immunoassay].an enzyme-linked immunosorbent assay using polyclonal antibodies from rabbits has been developed for quantification of acinetobacter calcoaceticus. bacteria were added to the wells of a microtiter plate coated with anti-acinetobacter immunoglobulin. for detecting bound cells the peroxidase-labelled immunoglobulin fraction was used. over a distinct range there is a linear correlation between bound bacteria and measured absorbance allowing a quantification of bacteria in an order from 10(7) to 10( ...19911793089
release of transforming plasmid and chromosomal dna from two cultured soil bacteria.the release of chromosomal and plasmid dna from acinetobacter calcoaceticus and bacillus subtilis cultivated in minimal medium and broth over a period of 50 h was monitored and related to growth phase, autolysis, dnase production and natural competence. the released dnas were biologically active in natural transformation. in addition, the circular integrity of a released b. subtilis shuttle vector (phv14) was demonstrated by artificial transformation of escherichia coli. in cultures of both stra ...19911793338
[susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ...19911798067
[sensitivity of acinetobacter calcoaceticus-baumanni complex and acinetobacter lwoffi to common antibiotics]. 19911799617
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
[imipenem-cilastatin cure of meningitis caused by acinetobacter calcoaceticus]. 19911805958
mechanisms of resistance to beta-lactam antibiotics in acinetobacter calcoaceticus.examination of 12 strains of acinetobacter calcoaceticus revealed that the strains expressed different constitutive levels of beta-lactamase. mutants resistant to cefoxitin, cefoperazone or ceftazidime were selected from a strain producing a low level of beta-lactamase. all the mutants showed no change in expression of beta-lactamase, but produced penicillin-binding proteins with altered expression and/or affinity for beta-lactams. in addition, the outer membrane of the mutants showed decreased ...19911816177
[sensitivity of bacteria of the genus acinetobacter to antibiotics and ofloxacin].between 1989-1989 276 strains of acinetobacter genus were isolated which contained: acinetobacter calcoaceticus subsp. anitratus (n = 167), acinetobacter calcoaceticus subsp. iwoffi (n- = 83), acinetobacter haemolyticus (n = 26). their sensitivity to aminoglycoside antibiotics, beta-lactams, tetracyclines, chloramphenicol, colistin, and ofloxacin was tested. more than 90% of strains were sensitive to colistin and ofloxacin. the sensitivity to remaining antibiotics differentiated depending on spe ...19911823372
[acinetobacter in a metropolitan neonatal unit: clinical and microbiological aspects].acinetobacter calcoaceticus var. anitratus (aca) was isolated from two newborn infants and var. iwoffi of the same species from a third patient within a month in the neonatal unit of a general hospital at metropolitan santiago, chile. the first isolate was obtained from a neonate with infection of the skin surrounding a colostomy operation after surgery for tracheo esophageal fistulae, in march 1989. the second case's skin became colonized after he was admitted for surgical repair of severe gast ...19911844163
[intrahospital septicemia due to acinetobacter calcoaceticus var. anitratus in a neonatal intensive care unit].twenty-one cases of sepsis by acinetobacter calcoaceticus biovar anitratus occurred, ten in 1989 and eleven in 1990 (january-april). in this last year, there were nine cases with characteristics of an epidemic outbreak. it was noted that sepsis was predominant in males, babies of less than 2,500 g and less than 37 weeks of gestation. they were hospitalized in our intensive care unit for different problems, but almost all had mechanical ventilation, parenteral nutrition and received antibiotics p ...19911844938
susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to newer antimicrobials.the susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to a variety of newer antimicrobials was determined. sixty-three amikacin-resistant isolates were collected from 61 inpatients at a tertiary-care hospital between 1985 and 1988. the organisms included xanthomonas maltophilia (24 isolates), pseudomonas aeruginosa (16), pseudomonas species (3), serratia marcescens (10), flavobacterium meningosepticum (4), enterobacter species (3), acinetobacter calcoaceticu ...19911860303
nucleotide sequences of the acinetobacter calcoaceticus benabc genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases.the nucleotide sequences of the acinetobacter calcoaceticus benabc genes encoding a multicomponent oxygenase for the conversion of benzoate to a nonaromatic cis-diol were determined. the enzyme, benzoate 1,2-dioxygenase, is composed of a hydroxylase component, encoded by benab, and an electron transfer component, encoded by benc. comparison of the deduced amino acid sequences of benabc with related sequences, including those for the multicomponent toluate, toluene, benzene, and naphthalene 1,2-d ...19911885518
in vitro and in vivo evaluation of ro 09-1428, a new parenteral cephalosporin with high antipseudomonal 09-1428, a new parenteral cephalosporin with a catechol moiety attached at position 7 of the cephalosporin ring, showed high in vitro activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, and streptococcus pyogenes, with mics for 90% of strains tested (mic90s) of less than or equal to 0.39 micrograms/ml. morganella morganii, providencia rettgeri, citrobacter freundii, haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae were inhi ...19911906261
community acquired pneumonia: aetiology and prognostic index evaluation.a 10 month prospective study of all adults admitted to waikato hospital with community acquired pneumonia was performed to assess aetiology, mortality, hospital stay, and the value of a prognostic index based on that obtained from a british thoracic society study. the 92 patients in the survey had a mean age of 56 (range 13-97) years. a microbiological diagnosis was established in 72%; streptococcus pneumoniae (33%), mycoplasma pneumoniae (18%), and influenza a virus (8%) were the most common mi ...19911907034
[antimicrobial activity of cefpiramide to fresh clinical isolates].in the subjects of 835 strains of 37 clinically isolated microbial strains, which were separated and identified among materials collected from patients with various infections and which were sent from medical therapeutic institutions throughout japan in 1990, for the purpose of examining the antimicrobial activity of cefpiramide (cpm), its minimum inhibitory concentration (mic), together with those of other cephem antibiotics, was determined, and the following conclusions were obtained. 1. micro ...19911920808
imipenem/cilastatin as secondary therapy for bacterial infections.imipenem/cilastatin (ipm/cs) was used to treat 39 documented infections in patients who had failed to respond to other antibiotic regimens. the overall response rating was 76.9%. respiratory infections responded less frequently (efficacy rating, 55.6%) to ipm/cs than abdominal infections, urinary tract infections, or sepsis. methicillin-resistant staphylococcus aureus, xanthomonas maltophilia, and acinetobacter calcoaceticus were less sensitive to ipm/cs therapy than the other bacterial strains ...19911933995
purification and characterization of acinetobacter calcoaceticus isocitrate lyase.acinetobacter calcoaceticus is capable of growing on acetate or compounds that are metabolized to acetate. during adaptation to growth on acetate, a. calcoaceticus b4 exhibits an increase in nadp(+)-isocitrate dehydrogenase and isocitrate lyase activities. in contrast, during adaptation to growth on acetate, escherichia coli exhibits a decrease in nadp(+)-isocitrate dehydrogenase activity that is caused by reversible phosphorylation of specific serine residues on this enzyme. also, in e. coli, i ...19911938889
potential dna slippage structures acquired during evolutionary divergence of acinetobacter calcoaceticus chromosomal benabc and pseudomonas putida tol pww0 plasmid xylxyz, genes encoding benzoate dioxygenases.the xylxyz dna region is carried on the tol pww0 plasmid in pseudomonas putida and encodes a benzoate dioxygenase with broad substrate specificity. the dna sequence of the region is presented and compared with benabc, the chromosomal region encoding the benzoate dioxygenase of acinetobacter calcoaceticus. corresponding genes from the two biological sources share common ancestry: comparison of aligned xylx-bena, xyly-benb, and xylz-benc amino acid sequences revealed respective identities of 58.3, ...19911938949
microbial growth and endotoxin production in the intravenous anesthetic this study, we measured microbial growth and endotoxin production in the intravenous anesthetic propofol using 10 different microbial strains; 6 isolated from outbreak cases and 4 from laboratory stock cultures.19911940276
association of contaminated gloves with transmission of acinetobacter calcoaceticus var. anitratus in an intensive care unit.acinetobacter calcoaceticus var. anitratus is an important nosocomial pathogen that has been associated with environmental reservoirs. an increased isolation rate of a. anitratus in our intensive care units (icus), from 0.03% (two of 7,800) to 0.5% (seven of 1,300) (p less than 0.00003), prompted an investigation.19911951409
[antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections].in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ...19911960856
a sheep survived for 48 days with the biventricular bypass type total artificial heart.a biventricular bypass type total artificial heart (bvb-tah) utilizing two pusher-plate pumps was developed and implanted in a sheep for 48 days with excellent results. a hall effect sensor was utilized to operate each pump independently with a full stroke at variable rates (vr). with this system, the animal's hemodynamics was kept physiologically, and all metabolic parameters except hemoglobin and hematocrit returned to normal three weeks after implantation. however, signs of infection appeared ...19911962530
inducible high-level expression of heterologous genes in bacillus megaterium using the regulatory elements of the xylose-utilization operon.we have constructed a shuttle plasmid for bacillus megaterium and escherichia coli that contains the promoter and repressor gene of the b. megaterium-borne operon for xylose utilization. a polylinker downstream of the promoter allows versatile cloning of genes under its transcriptional control. we have placed gdha (encoding glucose dehydrogenase) from b. megaterium, lacz (encoding beta-galactosidase) from e. coli, mro (encoding mutarotase) from acinetobacter calcoaceticus, and human puk (encodin ...19911367576
cloning and nucleotide sequences of the acci restriction-modification genes in acinetobacter calcoaceticus.the genes of the acci restriction-modification system specific for gt(a/c) (g/t)ac were cloned from the chromosomal dna of acinetobacter calcoaceticus, and their nucleotides sequenced. the restriction and modification genes coded for polypeptides with calculated molecular weights of 42,494 and 63,078, respectively. both the enzymes were coded by the same dna strand and the restriction gene was upstream of the methylase gene, separated by 2 bp. the restriction gene was significantly expressed in ...19911368703
efficacy of teicoplanin and imipenem on respiratory infections in high risk patients.the efficacy of teicoplanin and imipenem was assayed on microorganisms, mainly hospital opportunistic organisms, resistant to common therapy, isolated from bronchoaspirates of high risk hospitalized patients. imipenem has shown remarkable activity against all isolated microorganisms with sensitivities ranging from 91.93% against acinetobacter calcoaceticus to 75.86% against pseudomonas sp. teicoplanin has shown excellent activity against staphylococcus aureus (sensitivity 83.66%).199112041770
ribotyping of the acinetobacter calcoaceticus-acinetobacter baumannii complex.the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four genotypically distinct but phenotypically very similar bacterial species or dna groups: a. calcoaceticus (dna group 1), a. baumannii (dna group 2), unnamed dna group 3 (p. j. m. bouvet and p. a. d. grimont, int. j. syst. bacteriol. 36:228-240, 1986), and unnamed dna group 13 (i. tjernberg and j. ursing, apmis 97:595-605, 1989). because strains in this complex cause nosocomial outbreaks, it is important to be able to ...19921383266
long-chain alcohol and aldehyde dehydrogenase activities in acinetobacter calcoaceticus strain ho1-n.three alcohol dehydrogenases have been identified in acinetobacter calcoaceticus sp. strain ho1-n: an nad(+)-dependent enzyme and two nadp(+)-dependent enzymes. one of the nadp(+)-dependent alcohol dehydrogenases was partially purified and was specific for long-chain substrates. with tetradecanol as substrate an apparent km value of 5.2 microm was calculated. this enzyme has a pi of 4.5 and a molecular mass of 144 kda. all three alcohol dehydrogenases were constitutively expressed. three aldehyd ...19921402794
analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ...19921447059
characterization of a high-affinity iron transport system in acinetobacter baumannii.analysis of a clinical isolate of acinetobacter baumannii showed that this bacterium was able to grow under iron-limiting conditions, using chemically defined growth media containing different iron chelators such as human transferrin, ethylenediaminedi-(o-hydroxyphenyl)acetic acid, nitrilotriacetic acid, and 2,2'-bipyridyl. this iron uptake-proficient phenotype was due to the synthesis and secretion of a catechol-type siderophore compound. utilization bioassays using the salmonella typhimurium i ...19921447137
in vitro and in vivo activities of dq-2556 and its mode of action.dq-2556 is a recently developed cephalosporin with a broad spectrum of antibacterial activity against both gram-positive and gram-negative bacteria. its in vitro activity was roughly comparable to those of cefuzonam and cefpirome and greater than those of ceftazidime, cefepime, and cefclidin against gram-positive bacteria. against gram-negative bacteria, dq-2556 showed almost the same activity as those shown by cefpirome and cefepime. the activity was largely unaffected by culture medium ph or t ...19921482128
[antimicrobial activities of ceftazidime on fresh clinical isolates].antimicrobial activity of ceftazidime (caz) was compared with those of other cephem antibiotics against clinically isolated strains sent to us by medical institutions throughout japan in 1989 and 1991. those strains separated and identified from samples collected from patients with various infections were also examined, and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic90 of caz in 1991 we ...19921494227
purification of a periplasmic insulin-cleaving proteinase from acinetobacter calcoaceticus.cells of acinetobacter calcoaceticus contain a constitutive periplasmic metalloproteinase showing similar properties as the periplasmic metalloproteinase of escherichia coli. the periplasmic proteinase of a. calcoaceticus was purified, starting from periplasm, by ammonium sulfate precipitation, hydrophobic interaction chromatography and chromatofocusing up to the homogeneity of the enzyme in sds-electrophoresis with a yield of 6.7% and a purification factor of 417. the enzyme has a molecular mas ...19921510571
co-existence of beta-lactamase and penicillin acylase in bacteria; detection and quantitative determination of enzyme activities.twenty-six bacteria were examined for the presence of penicillin acylase and beta-lactamase. a copper reducing assay, which was sensitive in the analytical range 2-20 micrograms/ml, was used for determination of penicilloates and a fluorescamine assay was used to determine 6-aminopenicillanic acid concentrations when both substances were produced by the action of the enzymes on a single substrate. seventeen bacteria contained beta-lactamases, six contained penicillin acylases and four contained ...19921512174
purification and characterization of l-2,4-diaminobutyrate decarboxylase from acinetobacter calcoaceticus.acinetobacter calcoaceticus atcc 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. the enzyme responsible, l-2,4-diaminobutyrate (daba) decarboxylase (ec 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. the native enzyme had an m(r) of approximately 108,000, with a pi of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent m(r) 53,000. the enzyme showed hyperbolic kinetics with a km of 1.59 mm for daba a ...19921512577
microbial contamination of enteral feeding solution and its an investigation of microbial contamination of enteral feeding solutions, all 22 residual solutions obtained immediately after administration were contaminated at concentrations of 10(3) to 10(6) viable counts/ml. major contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas aeruginosa and acinetobacter calcoaceticus var anitratus. contamination seemed to have been caused by frequent reuse of bag-type containers and the infusion tubes connected to the bags, neither o ...19921524268
purification and characterization of an extracellular beta-lactamase produced by acinetobacter calcoaceticus.a beta-lactamase was purified 430-fold from the culture supernatant of acinetobacter calcoaceticus by ion exchange chromatography on cm-sephadex and affinity chromatography on phenylboronic-acid-agarose. the purified enzyme was homogeneous as judged by sds-page, and was characterized with respect to molecular mass (38 and 41 kda by gel filtration on sephadex g-75 and sds-page, respectively), ph optimum (ph 7.0), temperature optimum (45 degrees c) and isoelectric point (9.3). the beta-lactamase s ...19921527494
plasmid transformation of naturally competent acinetobacter calcoaceticus in non-sterile soil extract and groundwater.the natural transformation of acinetobacter calcoaceticus bd413 (trpe27) was characterized with respect to features that might be important for a possible gene transfer by extracellular dna in natural environments. transformation of competent cells with chromosomal dna (marker trp+) occurred in aqueous solutions of single divalent cations. uptake of dna into the dnase i-resistant state but not the binding of dna to cells was strongly stimulated by divalent cations. an increase of transformation ...19921590708
infective endocarditis of a native valve due to acinetobacter: case report and review.a case of community-acquired infective endocarditis of a native valve that was caused by acinetobacter calcoaceticus subspecies anitratus is presented. the previously reported cases are reviewed, and therapy for this disorder is discussed. the presence of a transient maculopapular rash involving the palms and soles but sparing the face is suggested as a possible early clinical clue to the diagnosis. native valve endocarditis caused by acinetobacter species is an acute, aggressive illness that is ...19921600019
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...19921629326
characterization of transformation-deficient mutants of acinetobacter calcoaceticus.three acinetobacter calcoaceticus transformation-deficient mutants, obtained by insertional mutagenesis with the nptll gene, have been characterized physiologically. one mutant (aac211) was found to be completely transformation deficient, while two others, aac213 and aac214, were severely impaired in transformation efficiency (100-1000 times lower than the wild type). the latter applied to both chromosomal as well as plasmid dna. analysis of the chromosomal dna fragments flanking the nptll gene ...19921630314
outer membranes of gram-negative bacteria are permeable to steroid probes.the permeability of bacterial outer membranes was assayed by coupling the influx of highly hydrophobic probes, 3-oxosteroids, with their subsequent oxidation catalysed by 3-oxosteroid delta 1-dehydrogenase, expressed from a gene cloned from pseudomonas testosteroni. in salmonella typhimurium producing wild-type lipopolysaccharide, the permeability coefficients for uncharged steroids were 0.45 to 1 x 10(-5) cm s-1, and the diffusion appeared to occur mainly through the lipid bilayer domains of th ...19921640833
in vitro and in vivo antibacterial activities of meropenem, a new carbapenem antibiotic.the in vitro and in vivo antibacterial activities of meropenem were compared with those of imipenem, ceftazidime, flomoxef, cefuzonam and cefotiam. meropenem showed a broad antibacterial spectrum against clinical isolates of gram-positive and gram-negative bacteria. against gram-negative bacteria, with the exception of acinetobacter calcoaceticus, meropenem exhibited the most potent activity among the drugs tested. it inhibited all 330 strains of enterobacteriaceae at 0.78 mg/l. meropenem was se ...19921644010
leprosy in hiv-positive and syphilitic young paraguayan a borderline lepromatous patient a positive serology for syphilis and hiv 1 has been detected. the patient also had an urinary tract infection with acinetobacter calcoaceticus. in paraguay up till now no leprosy cases infected with hiv have been reported.19921293911
a simultaneous bilateral attack of infectious corneal ulcers in an extended-wear soft contact lens wearer: a case report.a case study was made of a 23-year-old female with simultaneous bilateral infectious corneal ulcers related to extended-wear soft contact lenses. cultures revealed pseudomonas aeruginosa and acinetobacter calcoaceticus. multiple high risk factors were combined, including extended-wear, poor lens care, bilateral alternate wearing, and smoking. amphetamine addiction may also have played a role. after treatment, a visual acuity of 20/20 was achieved in both eyes by the use of rigid gas-permeable co ...19921296043
antibacterial in vitro-activity of meropenem against 200 clinical isolates in comparison to 11 selected antibiotics.the antimicrobial activity of meropenem, a new parenteral carbapenem, was tested in vitro by an agar dilution method against 200 clinical isolates (gram-negative/positive aerobes and anaerobes). meropenem was compared with imipenem, ceftazidime, cefotaxime, piperacillin, ciprofloxacin, gentamicin; and metronidazole, cefoxitin, chloramphenicol, clindamycin, vancomycin when appropriate. meropenem and imipenem exhibited an extended spectrum of activity with low minimal inhibitory concentrations (mi ...19921303691
a 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme least four genes are required for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone (pqq) in acinetobacter calcoaceticus. the dna region where one of these genes was mapped codes for a polypeptide of only 24 amino acids. here we show that indeed this small peptide is essential for pqq synthesis. site-directed mutagenesis shows that at least one glutamate and one tyrosine residue of the polypeptide are essential for its function.19921310505
nucleotide sequence and structure of the klebsiella pneumoniae pqq operon.a 6940 bp klebsiella pneumoniae chromosomal dna fragment, containing genes involved in pyrroloquinoline quinone (pqq) biosynthesis, was sequenced. six open reading frames, pqqa, pqqb, pqqc, pqqd, pqqe and pqqf were identified in the pqq operon, which coded for polypeptides of 2764 (23 amino acids), 33,464, 28,986, 10,436, 42,881 and 83,616 da, respectively. the transcription startpoint was mapped by primer extension analysis, upstream of pqqa, and promoter boxes could be identified. the gene pro ...19921313537
plasmid profiles of acinetobacter and enterobacter species of hospital origin: restriction endonuclease analysis of plasmid dna and transformation of escherichia coli by r plasmids.a total of 37 multi-resistant strains (20 acinetobacter calcoaceticus and 17 enterobacter cloacae) were isolated from patients of the intensive care units. all the isolates were examined for resistance to a battery of antimicrobial agents by the disk diffusion method. plasmid profiles and restriction endonuclease analysis of plasmid dna by ecor1 revealed the spread of one a. calcoaceticus and two e. cloacae endemic strains. transformation experiments on escherichia coli competent cells by three ...19921321240
acli, a new restriction endonuclease from acinetobacter calcoaceticus recognizing 5'-a decreases cgtt-3'. 19921322531
cloning of an erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in escherichia coli hb101: nucleotide sequence and probable involvement in biosynthesis of the coenzyme pyrroloquinoline quinone.escherichia coli is capable of synthesizing the apo-glucose dehydrogenase enzyme (gdh) but not the cofactor pyrroloquinoline quinone (pqq), which is essential for formation of the holoenzyme. therefore, in the absence of exogenous pqq, e. coli does not produce gluconic acid. evidence is presented to show that the expression of an erwinia herbicola gene in e. coli hb101(pmcg898) resulted in the production of gluconic acid, which, in turn, implied pqq biosynthesis. transposon mutagenesis showed th ...19921325965
in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ...19921332587
epidemiological role of arthropods detectable in health facilities.a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ...19921350604
genetic analysis of supraoperonic clustering by use of natural transformation in acinetobacter calcoaceticus.dna within escherichia coli colonies carrying cloned acinetobacter calcoaceticus genes transforms mutant a. calocaceticus cells with high efficiency. therefore, e. coli colonies containing such cloned genes can be identified by replica plating onto a lawn of a. calcoaceticus mutant cells. transformation of a. calcoaceticus also facilitates gap repair and thus allows recovery of specified chromosomal segments in recombinant plasmids. these procedures were used to demonstrate the clustering of a. ...19921729209
phosphorylation of acinetobacter isocitrate lyase.during growth on succinate, acinetobacter calcoaceticus contains two forms of the enzyme isocitrate dehydrogenase. addition of acetate to a lag-phase culture grown on succinate causes a dramatic increase in activity of form ii of isocitrate dehydrogenase and in isocitrate lyase. form ii of isocitrate dehydrogenase may be responsible for the partition of isocitrate between the tca cycle and the glyoxylate by-pass. this report describes the phosphorylation of the enzyme isocitrate lyase from a. ca ...19921731794
cis-diol dehydrogenases encoded by the tol pww0 plasmid xyll gene and the acinetobacter calcoaceticus chromosomal bend gene are members of the short-chain alcohol dehydrogenase the aerobic degradation of benzoate by bacteria, benzoate is first dihydroxylated by a ring-hydroxylating dioxygenase to form a cis-diol (1,2-dihydroxycyclohexa-3,4-diene carboxylate) which is subsequently transformed to a catechol by an nad(+)-dependent cis-diol dehydrogenase. the structural gene for this dehydrogenase, encoded on tol plasmid pww0 of pseudomonas putida (xyll) and that encoded on the chromosome of acinetobacter calcoaceticus (bend), were sequenced. they encode polypeptides of ...19921740120
hydrocarbon degradation by acinetobacter calcoaceticus rag-1 using the self-cycling fermentation technique.the use of self-cycling fermentations (scfs) as a method for dealing with insoluble carbon substrates was examined. the emulsan-producing acinetobacter calcoaceticus rag-1 was used as the test organism. limiting concentrations of hexadecane, 1-hexadecene, or 1-chlorohexadecane were used as the carbon substrate. the parameters monitored were residual hydrocarbon concentration, cycle time (doubling time), biomass concentration and emulsan concentration. cycle-to-cycle variations of the measured pa ...199218601183
modeling and experimental validation of carbon dioxide evolution in alkalophilic cultures.the chemical reactions involving carbon dioxide in mineral culture media are considered. a mathematic model is set up, based on published data, which is valid at ph values below 9, and in which the nonideality of the solution is taken into account. the crucial parameter is the constant expressing the equilibrium between carbon dioxide and bicarbonate, k(1).the reactions were studied in three different aqueous solutions: water, mineral salt medium, and a suspension with nongrowing bacterial cells ...199218600908
self-cycling fermentation in a stirred tank reactor.self-cycling fermentations (scfs) were conducted in a stirred tank apparatus using bacillus subtilis and acinetobacter calcoaceticus. the systems were very stable and the experiments lasted through many cycles. the variation of parameters such as biomass and doubling time from cycle to cycle was small. the stirred tank reactor (str) allowed a much better control of the working volume in the fermentor from cycle to cycle, compared to the cyclone column, and it was not necessary to make periodic c ...199318609666
natural transformation of acinetobacter calcoaceticus by plasmid dna adsorbed on sand and groundwater aquifer is known that plasmid dna and linear duplex dna molecules adsorb to chemically purified mineral grains of sand and to particles of several clay fractions. it seemed desirable to examine whether plasmid dna would also adsorb to nonpurified mineral materials taken from the environment and, particularly, whether adsorbed plasmid dna would be available for natural transformation of bacteria. therefore, microcosms consisting of chemically pure sea sand plus buffered cacl(2) solution were compared ...199316348943
ci-990 (pd 131112): a new quinolone vitro susceptibility of 1021 strains of recent clinical isolates was determined against the new fluoroquinolone ci-990 (pd 131112) and compared with ci-960, ciprofloxacin, fleroxacin, lomefloxacin, norfloxacin, perfloxacin, sparfloxacin, and temafloxacin. the minimum inhibitory concentrations (mic) of ci-990 in mg/l required for >90% isolates were 0.03-0.5 for members of enterobacteriaceae, 0.12-2.0 for pseudomonas aeruginosa, 0.03-1.0 for aeromonas hydrophilia, 1.0-2.0 for acinetobacter calc ...199317588022
acinetobacter meningitis.10,468 csf samples from cases of meningitis in different age groups were cultured during 1988-1991. acinetobacter calcoaceticus was isolated in 12 (5.6%) of 211 positive cultures. the strain were 100% resistant to ampicillin, cotrimoxazole and tetracycline 50% resistant to cephazolin gentamicin and kanamycin but 100% susceptible to chloramphenicol.19938258473
comparison of three typing methods in hospital outbreaks of acinetobacter calcoaceticus infection.during a period of 11 months acinetobacter baumanii was isolated from 27 patients and 21 environmental samples in the intensive care unit (icu) and in one surgical unit of a university hospital. the isolates were characterized by biotyping, antibiograms and plasmid profiles and compared with co-isolates. plasmid fingerprinting distinguished three outbreaks, whereas other typing methods were less sensitive and discriminatory. although plasmid profiles seem to be a simple and reproducible marker f ...19938097217
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