acinetobacter meningitis - a diagnostic pitfall. a report of 3 cases.three fatal cases of meningitis caused by acinetobacter calcoaceticus var. anitratus are reported, 1 in a premature infant who was being artificially ventilated and 2 in adults who had had neurosurgical operations. the microscopic resemblance of this organism to neisseria meningitidis is high-lighted and the problems of antimicrobial therapy are discussed.19826801786
[rubredoxin reductase in crude extracts of acinetobacter calcoaceticus in relation to carbon source and growth phase].by immunization of rabbits with discelectrophoretically purified rubredoxin-reductase from ac. calcoaceticus an antiserum against this enzyme was prepared. the antiserum was monospecifical to a diaphoretic activity, which had a rf-value identical with the rubredoxin-reductase-rf value. it has been shown by discelectrophoresis, immunoelectrophoresis, ouchterlony technique and kinetic investigations that crude extracts of bacteria grown on c16, malate, succinate or acetate contain rubredoxin-reduc ...19826803449
the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1.the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. in the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. the kinetics of antiserum-induced agglutination was quantified by measuring the time-dependent decrease in turbidity. for mutant isolation, a sample of the cells remaining in the supernata ...19816806434
[studies on acinetobacter calcoaceticus. the virulence and the therapeutic efficacy of antibiotics in mice]. 19826816860
[studies on acinetobacter calcoaceticus. protective effects of antiserum and vaccine to infection]. 19826816861
outbreak of pyrogenic reactions at a dialysis center. association with infusion of heparinized saline solution.twenty-three pyrogenic reactions occurred in 16 patients undergoing hemodialysis at a private dialysis center in the south central united states between november 23 and december 2, 1978. no deaths were attributed to reactions; however, 10 patients were hospitalized for observation after experiencing a reaction. cultures of all blood specimens obtained from the patients gave negative results. chills (75 percent), nausea and/or vomiting (30 percent), and fever (90 percent) were the most common sig ...19836829590
reversible effects of cross-linking on the regulatory cooperativity of acinetobacter citrate synthase.citrate synthase was purified from acinetobacter calcoaceticus and treated with the cleavable cross-linking reagent dithiobis(succinimidyl propionate). cross-linking of the enzyme resulted in the abolition of the sigmoidal responses to inhibition by nadh and re-activation by amp displayed by the native enzyme. inhibition and re-activation were still observed but without any cooperativity. cleavage of the disulphide bonds in the cross-links by treatment with dithiothreitol restored the sigmoidal ...19836832355
[bacteria of the species acinetobacter calcoaceticus as the possible etiological factor in infectious complications in burns].the results of the study of the etiological role of acinetobacter calcoaceticus in infectious complications of burning lesions are presented. these bacteria were shown to occur in 28% of burn wounds, quite frequently as a monoculture or association with diphtheroids. a. calcoaceticus were capable of persisting in the wounds during the whole period of treatment (up to 2-3 months). a high level of the contamination of burn wounds with these microorganisms, reaching 10(5)-10(6) cells per 1 g of tis ...19836837196
[acinetobacter calcoaceticus strains in the environmental objects of a burn unit]. 19836840587
biological activities of the lipopolysaccharide and lipid a from acinetobacter calcoaceticus. 19836842571
acinetobacter mediastinitis and pneumonia in a thorotrastoma patient. the oropharyngeal flora as source of infection.a case of bilateral pneumonia, mediastinitis and septicaemia caused by acinetobacter calcoaceticus and candida albicans is described. the infections occurred after a palliative operation for an esophagotracheal fistula in a thorotrastoma patient. the oropharynx was colonized by the two microorganisms at admission and is presented as the source of these infections. clinical management and antimicrobial policy, including oropharyngeal decontamination, leading to a good outcome are reported.19836863724
acinetobacter calcoaceticus--a pathogen? 19836886450
emulsan production by acinetobacter calcoaceticus in the presence of chloramphenicol.when exponentially growing cultures of acinetobacter calcoaceticus rag-1 or rag-92 were either treated with inhibitors of protein synthesis or starved for a required amino acid, there was a stimulation in the production of emulsan, an extracellular polyanionic emulsifier. emulsan synthesis in the presence of chloramphenicol was dependent on utilizable sources of carbon and nitrogen and was inhibited by cyanide or azide or anaerobic conditions. radioactive tracer experiments indicated that the en ...19826896872
progressive resistance in a single strain of acinetobacter calcoaceticus recovered during a nosocomial outbreak.a study was undertaken to investigate a nosocomial outbreak of acinetobacter calcoaceticus. the outbreak occurred in a shock-trauma intensive care unit (icu) during a 6-month period and involved a total of 37 cases. although no deaths were attributable to acinetobacter, 60% of patients from whom acinetobacter was isolated were determined to have a lower respiratory tract infection. a significant difference (p less than 0.05) was noted between the length to stay in the unit of patients who acquir ...19826919395
comparative in vitro study of temocillin (brl 17421), a new penicillin.the activity of temocillin (brl 17421), a new penicillin, was tested in vitro against 653 isolates of gram-negative bacilli and gram-positive cocci. the drug was compared with other beta-lactam antibiotics and tobramycin. it inhibited the majority of gram-negative bacilli tested except for pseudomonas aeruginosa and acinetobacter calcoaceticus, which were highly resistant. it was active against more than 50% of the multiresistant strains tested. temocillin was more active than mezlocillin agains ...19826919412
a common source outbreak of acinetobacter pulmonary infections traced to wright respirometers.over a 30-day period, acinetobacter calcoaceticus var. antiratus was the responsible pathogen for hospital-acquired pneumonia in 10 patients, and resulted in the colonization of the upper respiratory tract in an additional 9 patients. wright respirometers contaminated by this organism were shown to be the common source for the outbreak as indicated by the recovery of a single serotype (8j), the inability to recover acinetobacter from any other environmental source, and the demonstration that moi ...19806930637
purification and characterization of rhodanese from acinetobacter calcoaceticus.rhodanese (thiosulfate : cyanide sulfur transferase, ec 2,8,1,1) was found to be contitutively present as an intracellular enzyme in acinetobacter calcoaceticus. the soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, cm-sephadex batchwise separation, qae-50 ion exchange chromatography, and socrose density gradient ultracentrifugation. the enxyme had a molecular weight of approximately 35000 with a ph optimum of 8-8.5. activity was subs ...19806931629
a case of neonatal meningitis caused by acinetobacter calcoaceticus var anitratus. 19826981750
evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures.a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ...19806999020
studies on glucose nonfermentative gram-negative bacilli. ii. transferable drug resistance in acinetobacter calcoaceticus. 19807000977
solubilization and stabilization of l(+)-mandelate dehydrogenase from acinetobacter calcoaceticus. 19807004952
restriction mediated by pav2 affects the transfer of plasmids in acinetobacter calcoaceticus.the naturally occurring plasmid pav2 restricts the entry of the p class plasmid rp4 and the w class plasmids r388 and s-a into acinetobacter calcoaceticus strain ebf65/65 from escherichia coli. the w class plasmids only transfer from e. coli into pav2-strains. plasmid rp4 is modified in the presence of pav2 such that it is no longer restricted on entry into pav2 recipients of strain ebf65/65.19807021765
behaviour of bacteriophage mu in acinetobacter calcoaceticus ebf65/65.transfer of rp4::mu plasmids from escherichia coli k12 to acinetobacter calcoaceticus ebf65/65 was very inefficient compared with rp4 and was only detectable to strains of ebf65/65 lacking pav2, a cryptic plasmid thought to code for a restriction/modification system. rp4::mu-derived plasmids transferred from pav2-strains of ebf65/65 back to e. coli k12 were found to carry defective prophages which had lost the ability to produce detectable phage particles. re-transfer of these defective plasmids ...19807021766
trimethoprim-sulfamethoxazole therapy for infective endocarditis.a man with stenosis of the aortic valve acquired endocarditis after abdominal surgery. klebsiella pneumoniae and acinetobacter calcoaceticus were cultured from his blood. the blood cultures remained positive despite intravenous gentamicin and cephalothin to which the organisms were sensitive in vitro. ultimately, the blood was sterilized by a combination of gentamicin and trimethoprim-sulfamethoxazole taken orally. the course of the patient was complicated by cardiac arrest and pericardial tampo ...19817029718
quinoprotein alcohol dehydrogenase from a non-methylotroph, acinetobacter calcoaceticus.acinetobacter calcoaceticus grown on ethanol contains an nad(p)+-independent alcohol dehydrogenase which resembles methanol dehydrogenase from methylotrophic bacteria in many respects. likewise, the prosthetic group of this enzyme appears to be identical to that of methanol dehydrogenase, namely, pyrrolo quinoline quinone. the organism is unable to grow on methanol, which means that quinoprotein alcohol dehydrogenases are not restricted to methylotrophs. arguments are presented for the idea that ...19817033448
evaluation of the automicrobic system for identification of glucose-nonfermenting gram-negative rods.with the enterobacteriaceae plus biochemical card, the automicrobic system (vitek systems, inc., hazelwood, mo.) is purported to identify members of the family enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: pseudomonas aeruginosa, p. fluorescens, p. putida, p. maltophilia, p. cepacia, and acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). the latter capability was examined in this investigation. of 410 glucose-nonfermenting rods included in the ...19827040451
isolation, purification, and chemical analysis of the lipopolysaccharide and lipid a of acinetobacter calcoaceticus nctc 10305.the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305 (london) was obtained by a modified phenol/chloroform/light petroleum method from the bacterial cells and from the culture medium in yields of 1.6% and 2.2% respectively (based on the bacterial dry weight). on chemical analysis, both preparations proved to be identical. the lipopolysaccharide obtained from the cells was purified by repeated ultracentrifugation, electrodialysis, and precipitation with sodium chloride. it was free of ...19827060573
different forms of quinoprotein aldose-(glucose-) dehydrogenase in acinetobacter calcoaceticus.the ratios of the oxidation rates of aldose sugars, determined in cell-free extracts of acinetobacter calcoaceticus, vary with the strain and growth conditions used. three distinct forms of glucose dehydrogenase with different substrate specificities, occurring in variable proportions in these extracts, are responsible for this effect. one form is the already known "soluble glucose dehydrogenase", the other two forms are complexes containing enzyme and components of the respiratory chain. the pr ...19827065812
weigle reactivation in acinetobacter calcoaceticus. 19827071193
[acinetobacter colcoaceticus: its properties, identification key and a study of its role in acute intestinal diseases].the authors present their new scheme for the identification of acinetobacter calcoaceticus, requiring a lesser number of test, and the characterstics of 84 strains isolated from patients with acute intestinal diseases and from the environment. some strains are atypical. acinetobacter has been detected in 7.8 +/- 1.0% of patients with acute intestinal diseases. the authors' observations confirm that the detection of acinetobacter in washings from such patients is more convincing for etiological d ...19827080744
the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods.we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ...19827087800
hand carriage of aerobic gram-negative rods by health care personnel.a quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic gram-negative rods by various groups of health care workers and 104 control subjects. overall, 31% of health care workers carried aerobic gram-negative rods on their hands compared to 59% of control subjects (p < 0.001). enterobacter agglomerans accounted for 40% of the isolates, and other enterobacter spp. 7%. other organisms included acinetobacter ...19827097001
acinetobacter meningitis: acquired infection in a neonatal intensive care unit.a cluster of 4 cases of meningitis due to acinetobacter calcoaceticus var anitratus occurred during a 5-day period in a neonatal intensive care unit. three of the infants were preterm and all had a history of other medical problems. initiation of intravenous therapy with carbenicillin was accompanied by clinical recovery and a bacteriological cure. intensive bacteriological investigation failed to show a common source for the infections.19827103551
nosocomial bacteremia due to acinetobacter 1980, 13 patients with positive blood cultures for acinetobacter calcoaceticus were detected in an 800 bed university medical center. twelve of the 13 isolates were identified as acinetobacter calcoaceticus var. anitratus and one as var. lwoffi. in the same period there were 361 positive specimens of a. calcoaceticus. eight of the patients were classified as having significant bacteremia (group a) with a serious infection in which acinetobacter was considered a significant pathogen. five addi ...19827107014
glucose-nonfermenting gram-negative bacilli associated with clinical veterinary specimens.glucose-nonfermenting gram-negative bacilli (nfb) have been recognized recently as opportunistic pathogens of humans. with few exceptions, strains of nfb have not been considered important enough to be identified when isolated from animals. in this study, all nfb isolated during a 1-year period in a clinical veterinary microbiology laboratory were identified to determine their prevalence. of the 347 strains of nfb obtained, the most common species were pseudomonas aeruginosa, acinetobacter calco ...19827107835
[antibiotic sensitivity of acinetobacter calcoaceticus in patients with infected burns].sensitivity of acinetobacter calcoaceticus var. anitratum isolated from infected burn wounds was studied with respect to 24 antibiotics. it was found that the isolates were multiple drug resistant. sensitivity was observed at least to 8 out of 24 antibiotics. the majority of the isolates were resistant to 10--15 drugs. many strains were resistant to 16--17 antibiotics. 11 antibacterial drugs, i. e. tobramycin, sisomicin, gentamicin, amicacin, neomycin, carbenicillin, polymyxin m, erythromycin, t ...19827114831
bacteriuria caused by acinetobacter calcoaceticus biovars in a normal population and in general practice.a total of 14 strains of acinetobacter calcoaceticus biovar anitratus and 93 strains of a. calcoaceticus biovar lwoffi from a total of 97 patients participating in studies of significant bacteriuria were cultured. these studies involved an interview survey of asymptomatic significant bacteriuria and a multiple-center investigation of urinary tract infection diagnosed in general practice. antibiotic susceptibilities of the strains as determined by a disk diffusion technique were generally in agre ...19827130359
[optimization of culture conditions for acinetobacter calcoaceticus grown on n-alkanes in a laboratory fermenter].the growth conditions for the cultivation of acinetobacter calcoaceticus in a commercial laboratory fermenter have been optimized. this was done taking former results of shake cultures as a basis. at the end of the logarithmic phase up to 10 g/l dry weight were obtained. the following culture conditions have been used: the medium contained 10 ml/l n-alkane and 6 g/l nh4cl. the ph was adjusted to 6.5 and po2 employed to 70% saturation. a 12 times higher amount of dry weight was observed compared ...19827136011
[meningitis caused by acinetobacter calcoaceticus]. 19827144321
sensitivity of 341 non-fermentative gram-negative bacteria to seven beta-lactam antibiotics.susceptibility of 341 isolates of non-fermentative gram-negative bacteria to carbenicillin, piperacillin, cefoperazone, moxalactam, cefotaxime, ceftizoxime, and n-formimidoyl thienamycin was determined by the agar dilution and disc diffusion methods. piperacillin was the most active agent against pseudomonas aeruginosa, thienamycin the most active against pseudomonas fluorescens and pseudomonas putida, and moxalactam the most active against pseudomonas maltophilia. piperacillin and thienamycin w ...19827173181
pneumonia caused by acinetobacter calcoaceticus var. anitratus. 19827186001
infective endocarditis due to acinetobacter calcoaceticus. 19807205689
influence of growth phase and carbon source on the content of rubredoxin in acinetobacter calcoaceticus.the rubredoxin content of acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, l-malate) and on the growth phase was studied by means of a radioimmunoassay. the method used was specific for rubredoxin and acinetobacter calcoaceticus. the formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. after growth of acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not ...19807212930
aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans.garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ...19817240404
[epidemiology of acinetobacter calcoaceticus]. 19807243591
a comparison of the in vitro activity of cefoxitin and cephalothin against 7,312 clinical isolates.the antibacterial activity of cefoxitin and cephalothin was tested against 7,312 clinical isolates of aerobic pathogens. cefoxitin was found to have a broad spectrum of activity. among the gram-positive cocci, 99.1% of staphylococcus aureus, 94.4% of staphylococcus epidermidis and 98.1% of streptococci group b had a minimal inhibitory concentration of 16 microgram/ml or less, while only 4.6% of enterococci were sensitive to this concentration of cefoxitin. among the gram-negative rods 94.4% of e ...19817249786
adherence of acinetobacter calcoaceticus rag-1 to human epithelial cells and to hexadecane.the ability of acinetobacter calcoaceticus rag-1 to adhere to human epithelial cells was investigated and compared with its ability to adhere to a test hydrocarbon (hexadecane). rag-1, a microorganism originally isolated for growth on hydrocarbon, adhered to epithelial cells when grown under conditions which promote its adherence to hexadecane; similarly, rag-1 cells adhered poorly to epithelial cells when grown under conditions which cause the cells to possess low affinity towards hexadecane. a ...19817263064
naturally occurring plasmids in acinetobacter calcoaceticus: pav2, a plasmid which influences the fertility of the sex factor pav1.acinetobacter calcoaceticus strain ebf65/65 harbours a cryptic plasmid, pav2, which has been shown by electrophoretic separation on agarose gels to have a molecular mass of approximately 13.5 megadaltons (md). transfer of the previously described sex factor pav1 (hinchliffe & vivian, 1980 a,b) from the hospital strainjc17 into strains possessing pav2 occurs only at a low frequency, whereas transfer to similar strains lacking pav2 occurs at a much higher frequency. in ebf65/65, pav1 may be presen ...19807264602
microbial flora of in-use, display eye shadow testers and bacterial challenges of unused eye shadows.we surveyed 15 different brands of eye shadow on display for customer use in different retail stores for microbial contamination. this was the first reported microbial surveillance of in-use eye shadow display testers in retail establishments. cultures were obtained at each retail store. sterile dacron swabs were rolled and rubbed over the entire used surface of each shadow, and each inoculum was streaked onto the surfaces of blood agar plates. of the 1,345 individual samples obtained, 67% were ...19817283427
role of adherence in growth of acinetobacter calcoaceticus rag-1 on hexadecane.the high affinity of acinetobacter calcoaceticus rag-1 for liquid hydrocarbons permitted the isolation of a spontaneous nonadherent mutant, mr-481. strain mr-481 exhibited no significant affinity for three test hydrocarbons, yet resembled the wild type in many properties, including production of the extracellular emulsifying agent emulsan. to study the role of adherence in growth on hydrocarbons, rag-1 and mr-481 were compared for growth on hexadecane under conditions of limited agitation and at ...19817287631
a cluster of acinetobacter pneumonia in foundry a 3-month period, three men who had worked for 5 to 19 years as welders or grinders of steel castings in a foundry acquired pneumonia caused by acinetobacter calcoaceticus variety anitratus serotype 7j. two of the men died, and postmortem examination showed mixed-dust pneumoconiosis with iron particles in the lungs. a calcoaceticus variety anitratus serotype 7j was isolated from the air in the foundry but the source was not found. the prevalence of antibody titers of 64 or greater to the 7j s ...19817305146
acinetobacter calcoaceticus-an opportunistic pathogen. 19817338827
isolation of acinetobacter calcoaceticus from an aborted equine foetus. 19817342938
evidence of plasmid-mediated production of aminoglycoside-modifying enzymes not previously described in acinetobacter.two blood culture isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) were recently observed to be unusually resistant to aminoglycosides. each strain was found to contain aminoglycoside-modifying enzymes which have not been described previously in this species, including 2"-adenylyltransferase, 3"-adenylyltransferase, 3'-phosphotransferase-iii, and 3-acetyltransferase. treatment of one strain (h-s) with novobiocin led to a loss of resistance to multiple antimicrobial ...19807352749
regulation of phosphoenolpyruvate carboxylase from acinetobacter calcoaceticus by nucleotides. 19807353640
naturally occurring plasmids in acinetobacter calcoaceticus: a p class r factor of restricted host range.a naturally occurring transmissible plasmid, designated pav1, has been isolated in acinetobacter calcoaceticus. it specifies resistance to sulphonamides and is capable of mobilizing two non-transmissible resistance determinants for tetracycline and neomycin, respectively, within strains of a. calcoaceticus. it is incompatible with the p class r factors rp4 and r751 in a. calcoaceticus. on this basis we conclude that pav1 is a member of the p incompatibility group. however, unlike most other p gr ...19807365457
repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida.muconolactone delta-isomerase (ec and beta-ketoadipate enol-lactone hydrolase (ec mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ...19807391022
evolutionary divergence of co-selected beta-ketoadipate enol-lactone hydrolases in acinetobacter calcoaceticus.muconolactone isomerase (ec and beta-ketoadipate enol-lactone hydrolase (ec mediate consecutive catabolic steps in bacteria. separately inducible beta-ketoadipate enol-lactone hydrolases i and ii are formed in representatives of acinetobacter calcoaceticus. when subjected to deae-cellulose chromatography, acinetobacter enol-lactone hydrolase i displays heterogeneous behavior which, in whole or in part, appears to be due to modifications of sulfhydryl groups in the protein; the ...19807391023
homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases.gamma-carboxymuconolactone decarobxylase (ec and muconolactone isomerase (ec mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ...19807391024
pyrogenic reactions after inadvertent infusion of endotoxin during cardiac april 1976 the attack rate of chills and fever with or without falls in blood pressure increased in association with cardiac catheterizations. fevers were associated with coronary angiography and right and left heart catheterizations. blood cultures were negative, and reactions did not correlate with amounts of contrast materials infused or with procedures done by a single operator. significant numbers of acinetobacter calcoaceticus (var. anitratus) and a pseudomonas species were cultured fro ...19807396310
gene transfer in acinetobacter calcoaceticus: fertility variants of the sex factor pav1.the naturally occurring transmissible plasmid pav1 mediates chromosome transfer and can exhibit two distinct levels of transmissibility in acinetobacter calcoaceticus strain ebf65/65. the two states of pav1 have been arbitrarily designated pav1a (low frequency variant) and pav1b (high frequency variant). both variants have the same incompatibility and host range properties and each mobilizes two non-transmissible resistance determinants for tetracycline and neomycin. sex factor activity has been ...19807411116
symptomatic acinetobacter calcoaceticus peritonitis. "a complication of peritoneal dialysis".acinetobacter calcoaceticus peritonitis was seen during the course of peritoneal dialysis in two patients with end-stage kidney disease within a 3-month period. in one patient, the isolate was a. calcoaceticus varient anitratus (formerly herellea vaginicola) and in the other it was a. calcoaceticus lwoffi (formerly mima polymorpha). both were successfully treated with continuous peritoneal antibiotic lavage.19807440841
meningitis caused by acinetobacter calcoaceticus var anitratus. a specific hazard in neurosurgical patients.acinetobacter calcoaceticus var anitratus caused meningitis in five patients between 1968 and 1978 at two hospitals affiliated with boston university school of medicine. all patients had had head trauma or neurosurgical procedures prior to the development of meningitis. the course of the disease was relatively indolent in that fulminant disease did not occur even when initial therapy was inappropriate and bacteria persisted in csf. all five patients survived. on gram's stain of csf, a calcoaceti ...19817469844
[the efficacy of sulbactam-ampicillin in a urinary infection due to acinetobacter calcoaceticus var. anitratus]. 19957475461
purification and characterization of acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in escherichia coli.4-hydroxybenzoate 3-hydroxylase [ec] from acinetobacter calcoaceticus was purified to homogeneity following the 40-fold overexpression of this gene (poba) in escherichia coli. overexpression was accomplished by placing the fola gene (encoding trimethoprim-resistant dihydrofolate reductase) directly downstream of the poba gene, and demanding growth of recombinants on elevated concentration of trimethoprim. presumably, the surviving variants have undergone a genetic alteration which allo ...19957490269
use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates.a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ...19937504429
a novel ancestral protein of drosophila alcohol dehydrogenase in streptomyces?polyclonal antibodies raised against purified drosophila alcohol dehydrogenase (adh) were used in western blot analyses to search for structurally and/or immunologically related proteins in prokaryotes and eukaryotes. no immunological-reactive protein was detected in a flesh fly, a locust, and butterflies. immunological similarity with the 50-kda pqq-glucose dehydrogenase (gludh)-b enzyme of acinetobacter calcoaceticus was found, but the cross-reactivity apparently is dependent on the high hydro ...19937510103
phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis.we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ...19947520730
pcr-based preparation of 23s rrna-targeted group-specific polynucleotide probes.dna coding for a variable region within domain iii of bacterial 23s rrna was used as the target for group-specific polynucleotide hybridization probes. the corresponding rdna was amplified in vitro by the pcr technique in combination with a pair of primers specific for flanking conserved target sites. the amplified fragments were cloned or used directly as probes. rna probes were generated by in vitro transcription of cloned or amplified rdna. the probes were labeled by incorporating modified nu ...19947524442
nucleotide sequence and functional analysis of the genes encoding 2,4,5-trichlorophenoxyacetic acid oxygenase in pseudomonas cepacia ac1100.pseudomonas cepacia ac1100 is able to use the chlorinated aromatic compound 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) as the sole source of carbon and energy. one of the early steps in this pathway is the conversion of 2,4,5-t to 2,4,5-trichlorophenol (2,4,5-tcp). 2,4,5-tcp accumulates in the culture medium when ac1100 is grown in the presence of 2,4,5-t. a dna region from the ac1100 genome has been subcloned as a 2.7-kb ssti-xbai dna fragment, which on transfer to pseudomonas aeruginosa pao1 ...19947527626
cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs.the two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. the gene cluster encoding this enzyme, cbdabc, was localized on a 70-kbp conjugative plasmid designated pbah1. the nucleotide sequences of cbdabc and flanking regions were determined. in the deduced amino acid sequence of the large subunit of the terminal o ...19957530709
comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of acinetobacter isolates.seventy-three isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex, including 26 isolates from 10 hospital outbreaks, were typed by ribotyping with ecori and clai and by pulsed-field gel electrophoresis (pfge) of genomic dna after digestion with apai. ribotyping with ecori distinguished 31 ribopatterns. digestion with clai generated another eight ribotypes. pfge, in contrast, identified 49 distinct patterns with seven variants. both methods detected all outbreak-related is ...19957542274
the in-vitro activity of cp 99,219, a new naphthyridone antimicrobial agent: a comparison with fluoroquinolone agents.the in-vitro activity of a new fluoroquinolone, cp 99,219 was compared with that of ciprofloxacin, du 6859, sparfloxacin and levofloxacin. cp 99,219 showed generally similar in-vitro activity to the other compounds tested against the enterobacteriaceae (mic90 of all agents < 0.5 mg/l except morganella morganii). it was found to be more active than ciprofloxacin, sparfloxacin and levofloxacin against the strains of acinetobacter calcoaceticus tested (mic90 1 mg/l). cp 99,219 was found to be four- ...19957559198
biosensor based on an enzyme modified electrode for highly-sensitive measurement of polyphenols.the use of glucose dehydrogenase from acinetobacter calcoaceticus for highly sensitive measurement of polyphenols, based on bioelectrocatalytic analyte recycling, has been demonstrated. a polyphenol (analyte) is oxidized on the surface of a glassy carbon electrode at an anodic potential and is regenerated by immobilized glucose dehydrogenase (gdh) in the presence of glucose, resulting in an amplified response. the dynamic properties of the enzyme-modified glassy carbon electrode allow the conven ...19957576439
catm encodes a lysr-type transcriptional activator regulating catechol degradation in acinetobacter calcoaceticus.on the basis of the constitutive phenotypes of two catm mutants of acinetobacter calcoaceticus, the catm protein was proposed to repress expression of two different loci involved in catechol degradation, cata and catbcijfd (e. neidle, c. hartnett, and l. n. ornston, j. bacteriol. 171:5410-5421, 1989). in spite of its proposed negative role as a repressor, catm is similar in amino acid sequence to positive transcriptional activators of the lysr family. investigating this anomaly, we found that in ...19957592340
unusual ancestry of dehydratases associated with quinate catabolism in acinetobacter calcoaceticus.catabolism of quinate to protocatechuate requires the consecutive action of quinate dehydrogenase (quia), dehydroquinate dehydratase (quib), and dehydroshikimate dehyratase (quic), genes for catabolism of protocatechuate are encoded by the pca operon in the acinetobacter calcoaceticus chromosome. observations reported here demonstrate that a. calcoaceticus qui genes are clustered in the order quibcxa directly downstream from the pca operon. sequence comparisons indicate that quix encodes a porin ...19957592351
distance between alleles as a determinant of linkage in natural transformation of acinetobacter calcoaceticus.cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor acinetobacter calcoaceticus dna. a different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. ligation of unlinked chromosomal dna fragments allowed them to be linked genetically through natural transformation.19957592360
characterization of the extracellular lipase, lipa, of acinetobacter calcoaceticus bd413 and sequence analysis of the cloned structural gene.the extracellular lipase from acinetobacter calcoaceticus bd413 was purified to homogeneity, via hydrophobic-interaction fast performance liquid chromatography (fplc), from cultures grown in mineral medium with hexadecane as the sole carbon source. the enzyme has an apparent molecular mass of 32 kda on sds-polyacrylamide gels and hydrolyses long acyl chain p-nitrophenol (pnp) esters, like pnp palmitate (pnpp), with optimal activity between ph 7.8 and 8.8. additionally, the enzyme shows activity ...19957596283
mixed meningitis: association of acinetobacter calcoaceticus var lwoffi and streptococcus faecium.we describe mixed bacterial meningitis in a young man due to acinetobacter calcoaceticus var lwoffi and streptococcus faecium. the combination of a calcoaceticus var lwoffi and s faecium as aetiology of mixed bacterial meningitis has not been previously reported. the patient recovered completely without neurologic sequelae on chloramphenicol and penicillin.19957596936
degradation of trans-ferulic and p-coumaric acid by acinetobacter calcoaceticus dsm 586.cell suspensions of acinetobacter calcoaceticus strain dsm 586 and dsm 590 were able to grow on benzoic, p-hydroxybenzoic and vanillic acid as sole carbon source. testing the utilization of trans-ferulic and p-coumaric acid, we found that the sole a. calcoaceticus dsm 586 efficiently degraded the lignocellulose related monomers. cells induced with trans-ferulic acid were able to oxidize trans-ferulic, p-coumaric, vanillic, p-hydroxybenzoic and protocatechuic acid at rates higher than the uninduc ...19957599157
[analysis of 1116 strains of pathogens isolated from infected burn wounds].we report the analysis of 1,116 strains of pathogens isolated from infected burn wounds of 536 patients hospitalized from 1989 to 1991. from the 1,116 strains of pathogens, 39 species of aerobes and fungi were found, including 217 strains of staphylococcus aureus, 208 strains of pseudomonas aeruginosa and 119 strains of acinetobacter calcoaceticus. the positive rates of the above three bacteria were 19.4%, 18.6% and 10.6% respectively. some opportunistic pathogens, such as bacillus cercus, aeroc ...19957600434
a transposon-like sequence adjacent to the acci restriction-modification operon.we have cloned and sequenced the accirm genes from weeksella zoohelcum (the original identification of this strain as acinetobacter calcoaceticus was incorrect). our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3' end of the dna methyltransferase-encoding gene (accim). we have sequenced approx. 3 kb beyond this operon. two genes were found, convergently transcribed with the r-m operon. the first of these genes encodes a ...19957607529
novel bioemulsifiers from microorganisms for use in foods.the main objective of this study was to test the range of microorganisms for production of extracellular, high molecular weight emulsifiers for potential use in foods. a standard emulsification assay developed specifically for assessing food emulsifiers was used to examine 24 extracellular microbial products from bacteria, yeasts and algae. of the 24 products tested, nine had emulsification ability that was as good as and eight had emulsifying properties that were better than those of the common ...19957632394
purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a klebsiella pneumoniae mutant strain.unlike the parent wild-type strain, the klebsiella pneumoniae mutant strain mao4 has a 4-hba+ phenotype. the capacity of this mutant to take up and metabolize 4-hydroxybenzoate (4-hba) relies on the expression of a permease and an nadph-linked monooxygenase (4-hba-3-hydroxylase). both enzymes are normally expressed at basal levels, and only the presence of 4-hba in the media enhances their activities. strikingly, when the acinetobacter calcoaceticus poba gene encoding 4-hydroxybenzoate-3-hydroxy ...19957646318
genomic species typing of acinetobacters by polymerase chain reaction amplification of the reca gene.the reca gene has been used as a target in screening for the presence of acinetobacters on the genospecies level and differentiation of relevant acinetobacter species from one another by pcr. primers deduced from known reca gene sequences of acinetobacter calcoaceticus and neisseria gonorrhoeae allowed the amplification of dnas from all acinetobacter genospecies. the size of the amplified dna fragment from all genospecies tested was approximately 435-500 bp relative to dna size markers. the ampl ...19957649456
a case of acinetobacter calcoaceticus pneumonia.a case of community acquired pneumonia with acinetobacter calcoaceticus is presented. acinetobacter must be considered in the differential diagnosis of gram negative coccobacillary pneumonia.19957660350
phosphate uptake by immobilized acinetobacter calcoaceticus cells in a full scale activated sludge in situ study of the p-uptake ability of acinetobacter calcoaceticus was carried out using the alginate immobilization technique. immobilized a. calcoaceticus cells displayed a high p-uptake ability (> 97% p-accumulating cells) when immersed in the aerobic zone of an activated sludge system for 30-240 min. the overall p-accumulation pattern of the anaerobic zone depicted a typical p-release mechanism. however, limited p-accumulation was also observed at this stage. growth and anaerobiosis wer ...19957662295
two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in acinetobacter calcoaceticus adp1.mutants of acinetobacter calcoaceticus adp1 unable to grow on dodecane, but retaining the ability to grow on lauric acid were isolated after ethylmethanesulphonate (ems) treatment. this growth deficiency was complemented by a clone from a gene library constructed from chromosomal dna of the wild-type strain. the complementing dna mapped in a gene encoding a polypeptide with homology to rubredoxins. the deduced putative rubredoxin amino acid sequence is more similar to related proteins from gram- ...19957670642
nk-lysin, a novel effector peptide of cytotoxic t and nk cells. structure and cdna cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity.a 78 residue antimicrobial, basic peptide, nk-lysin, with three intrachain disulfide bonds was purified from pig small intestine and characterized. a corresponding clone was isolated from a porcine bone marrow cdna library. the 780 bp dna sequence had a reading frame of 129 amino acids which corresponded to nk-lysin. the clone was used to show that stimulation with human interleukin-2 induced synthesis of nk-lysin-specific mrna in a lymphocyte fraction enriched for t and nk cells. lower levels o ...19957737114
a periplasmic insulin-cleaving proteinase (icp) from acinetobacter calcoaceticus sharing properties with protease iii from escherichia coli and ide from eucaryotes.a periplasmic insulin-cleaving proteinase (icp), purified to its electrophoretic homogeneity in the sds-page from the gram-negative bacterium acinetobacter calcoaceticus, was examined and compared in its properties with the protease iii (protease pi, pitrilysin, ec of escherichia coli and the insulin-destroying proteinase (ide, insulinase, ec from eucaryotes. the enzyme was proven to be a metalloprotease like protease iii and ide, as was shown by the inhibitory effects exer ...19957738784
different relative importances of the par operons and the effect of conjugal transfer on the maintenance of intact promiscuous plasmid rk2.the par region of the broad-host-range, incp alpha plasmid rk2 has been implicated as a stability determinant by its ability to enhance the maintenance of mini-rk2 plasmids or heterologous replicons in a growing population of host cells. the region consists of two operons: parcba, which encodes a multimer resolution system, and parde, which specifies a postsegregational response mechanism that is toxic to plasmidless segregants. to assess the importance of this region to the stable maintenance o ...19957751288
thermostable chimeric pqq glucose dehydrogenase.the thermal stability of pqq glucose dehydrogenases (pqqgdhs) which were chimeras with more than 95% made up of the n-terminal region of escherichia coli pqqgdh and the rest made up of the c-terminal region of acinetobacter calcoaceticus pqqgdh was investigated. among the chimeric pqqgdhs, e97a3 (e. coli 97% and a. calcoaceticus 3%) and e95a5 were found to possess higher thermal stability than parental e. coli pqqgdh. further detailed characterization of the thermal stability was carried out, fo ...19957758590
acinetobacter calcoaceticus liberates chromosomal dna during induction of competence by cell lysis.a transformation assay was used to assay the amount of dna present in the extracellular medium of a growing culture of acinetobacter calcoaceticus. it was observed that small amounts of dna were liberated during the entire exponential growth phase in a batch culture. release of dna could be fully accounted for by lysis of cells. lysis was quantified via simultaneous measurement of beta-galactosidase activity of cells and supernatant, with a strain that contained a plasmid (papa100) with lacz und ...19957765885
the glucose dehydrogenase-mediated energization of acinetobacter calcoaceticus as a tool for evaluating its susceptibility to, and defence against, hazardous chemicals.cells of acinetobacter calcoaceticus 69-v could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. the velocities of glucose oxidation-driven atp syntheses were relatively constant in the range from ph 5.4 to 7.5. with decreasing ph values (7.0, 6.0, 5.4) atp synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same ph value glucose oxidation was nearly unimpaired or inhibited more weakly. this finding is expressed by a de ...19957765916
characterization of lipase-deficient mutants of acinetobacter calcoaceticus bd413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase.acinetobacter calcoaceticus bd413 produces an extracellular lipase, which is encoded by the lipa gene. five lipase-deficient mutants have been generated via random insertion mutagenesis. phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in a. calcoaceticus. biochemical evidence classified four of the mutants as export mutants, which presumably are defective in translocation of the lipase across the outer membrane. the additional mutant, desig ...19957768830
elucidation of the region responsible for edta tolerance in pqq glucose dehydrogenases by constructing escherichia coli and acinetobacter calcoaceticus chimeric enzymes.we constructed various chimeric pqq glucose dehydrogenases (pqqgdhs) from an edta-sensitive pqqgdh from escherichia coli and an edta-tolerant pqqgdh from acinetobacter calcoaceticus by homologous recombination of their structural genes. the edta tolerance of the resulting chimeric enzymes was investigated. our results demonstrated that edta tolerance of pqqgdhs can be completely altered by substituting each corresponding region. the edta tolerance of a. calcoaceticus pqqgdh is mostly within a re ...19957779095
characterization of the chromosomal aac(6')-ij gene of acinetobacter sp. 13 and the aac(6')-ih plasmid gene of acinetobacter baumannii.the amikacin resistance genes aac(6')-ih of acinetobacter baumannii bm2686 and aac(6')-ij of acinetobacter sp. 13 bm2689 encoding aminoglycoside 6'-n-acetyltransferases were characterized. the 441-bp coding sequences predict proteins with calculated masses of 16,698 and 16,677 da, respectively. analysis of the deduced amino acid sequences indicated that the proteins belonged to a subfamily of 6'-aminoglycoside acetyltransferase type i enzymes from gram-negative bacteria. the aac(6')-ih gene of b ...19947810994
in vitro antibacterial activity and beta-lactamase stability of sy5555, a new oral penem antibiotic.the antibacterial activity of sy5555, a new oral penem antibiotic, was compared with those of cefaclor, cefixime, and cefteram. sy5555 was more active than the comparison agents against methicillin-susceptible staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, enterococcus faecalis, citrobacter freundii, enterobacter cloacae, morganella morganii, acinetobacter calcoaceticus, clostridium spp., and bacteroides fragilis. against providencia spp., proteus spp., and haemophi ...19947811005
in-vitro activity of ampicillin/sulbactam against multiresistant acinetobacter calcoaceticus var. anitratus clinical isolates. 19947814296
acinetobacter calcoaceticus anitratus outbreak in the intensive care unit traced to a peak flow meter.a cluster of seven cases of acinetobacter caleoaceticus anitratus in a community teaching hospital intensive care unit was discovered (the seventh case was located in a step-down unit next to an infected patient recently transferred from the intensive care unit.)19947847640
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