TitleAbstractYear(sorted ascending)
effect of plasmid rp1 on phase changes in inner and outer membranes and lipopolysaccharide from acinetobacter calcoaceticus: a fourier transform infrared study.the successful transfer of the resistance plasmid rp1 into the gram-negative bacterium acinetobacter calcoaceticus resulted in increased resistance of this microorganism to the antibiotics kanamycin and tetracycline. microorganisms harboring the rp1 plasmid showed altered fatty acid composition in the lipopolysaccharide fraction and increased outer membrane permeability compared to organisms without the plasmid. thermotropic gel to liquid crystal lipid phase changes were detected in both inner a ...19873427032
typing of acinetobacter calcoaceticus strains isolated from hospital patients by cell envelope protein profiles.the usefulness of sodium dodecyl sulphate-polyacrylamide gel electrophoresis patterns of cell envelope proteins for classifying strains of acinetobacter calcoaceticus was studied using 129 isolates from 16 in-patients in a teaching hospital. in 11 patients, all of the isolates from each patient exhibited the same pattern irrespective of the body site or time of isolation. the patterns of the isolates from four other patients were indistinguishable, with the exception of one isolate per patient. ...19873428372
protein fingerprinting for the determination of relatedness in acinetobacter calcoaceticus subspecies anitratus isolated from patients in a surgical intensive care unit.the use of protein fingerprinting for the establishment of relatedness among isolates of acinetobacter calcoaceticus subspecies anitratus, isolated from patients in a surgical intensive care unit was examined. polyacrylamide gel electrophoresis was used to analyze the cellular proteins from whole cell lysates of 14 intensive care unit a calcoaceticus subspecies anitratus isolates and 11 control strains. antimicrobial susceptibilities and plasmid profiles were also determined for all isolates. al ...19873429162
[antibacterial activity of species of the genus allium].garlic, onion and shallot were tested for antimicrobial activity against pathogenic aerobic and anaerobic bacteria. the mic of aqueous and petroleum ether extracts were determined. garlic showed the greater activity; the combination garlic-antibiotic is synergistic against acinetobacter calcoaceticus and leads to indifference against anaerobic bacteria. the active constituent is not probably allicin alone.19873438321
enzymatic resistance to beta-lactams and aminoglycosides in acinetobacter calcoaceticus. 19873440769
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
[cytochrome p-450 and the respiratory activity of acinetobacter calcoaceticus growing on n-nonane]. 19873448466
pww174: a large plasmid from acinetobacter calcoaceticus encoding benzene catabolism by the beta-ketoadipate pathway.acinetobacter calcoaceticus rje74 contains a large transmissible catabolic plasmid, pww174, of about 200 kb, which encodes its ability to grow on benzene (bzn+). pww174 was unstable in acinetobacter hosts and was lost at high frequency in the absence of selection for bzn+. the catabolic pathway appeared to be via benzene cis-glycol, catechol and the beta-ketoadipate (ortho) pathway. pww174 encodes a catechol 1,2-oxygenase which is significantly more thermolabile than the chromosomally determined ...19873449741
isolation and characterization of the extracellular lipase of acinetobacter calcoaceticus 69 v.the extracellular lipase of acinetobacter calcoaceticus 69 v was purified by hydrophobic interaction chromatography to homogeneity as suggested by gel electrophoretic analysis. the lipase existed as a high molecular complex of about 300 kda, with a subunit molecular weight of 30.5 kda being obtained by sds-page. the hydrodynamic molecular radius obtained by gel electrophoresis was 3.27 nm. the lipase had an isoelectric point of 5.5 and was stimulated by additions of deoxycholate. the activation ...19873453402
combined resistance to quinolones and beta-lactams after in vitro transfer on single drugs.single and combined resistance to quinolones and beta-lactams was determined after serial transfers of 18 selected strains of pseudomonas aeruginosa (5), acinetobacter calcoaceticus (3) and beta-lactamase producing enterobacterial strains (10), in broth dilutions of 4 quinolones and 8 beta-lactams. two definitions for resistance were used: (i) 8-fold mic increase and stability of acquired resistance after five transfers in drug-free broth; and (ii) 8-fold mic increase over the breakpoint level. ...19873109816
adhesion of bacteria from mixed cell suspension to solid surfaces.the attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. three types of experiment were done: attachment of bacteria from suspensions containing two species (termed "simultaneous attachment") was compared to attachment of each species in pure culture, the attachment of one species of bacterium to surfaces already colonized by a second sp ...19873115220
[epidemiologic studies of the microbial colonization of severely burned patients].bacteria isolated between 1/1/1983 and 12/31/1984 from the burns of 122 patients in a burns unit at the ruhr-universität bochum were studied. grampositive bacteria were predominant in colonizing the burn wounds (62.5% of all strains isolated). in the patients with more than 40% of total body surface area (tbsa) burn, isolation of staph aureus was most frequent. the exogenous colonization rate with staph. aureus was 86%. coagulase negative staphylococci were identified in 89.6% of all patients (7 ...19873116791
serum bactericidal activity and postantibiotic effect in serum of patients with urinary tract infection receiving high-dose amikacin.ten patients received a 30-min infusion of amikacin (30 mg/kg) on day 1 and 15 mg/kg on day 2. mean serum creatinine was 1.1 +/- 0.3 (standard deviation) mg/dl before and 1.0 +/- 0.3 mg/dl 3 days after the second infusion. mean serum amikacin concentrations before, at the end of infusion, and 1, 6, 12, and 24 h after 30 and 15 mg/kg were 0, 157, 79, 31, 16, 5, 5, 85, 51, 19, 12, and 5 mg/liter, respectively. five strains each of staphylococcus aureus, staphylococcus epidermidis susceptible and r ...19873116918
studies on the chemical structure of the core-lipid a region of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305. detection of a new 2-octulosonic acid interlinking the core oligosaccharide and lipid a component.lipopolysaccharide of acinetobacter calcoaceticus nctc 10305 was treated with acid (0.1 m hcl, 100 degrees c, 1 h). the product obtained (lpsdegr) was subjected to various modification and degradation procedures including reduction, hydrazinolysis and strong acid hydrolysis. methylation analysis of purified part structures revealed the presence of a 4'-phosphorylated (beta 1'-6)-linked d-glucosamine disaccharide (lipid a backbone), which carried in position 6' a hitherto unknown 2-octulosonic ac ...19873830168
uv-inducible dna repair in acinetobacter calcoaceticus.bacterial mutation frequency after uv irradiation and phage mutation frequency under conditions of w-reactivation were determined in a. calcoaceticus. with the exception of streptomycin resistance, there was no increase in the frequency of the assayed markers above the background level. the increased survival of phage during w-reactivation was not followed by an increase in the frequency of mutation from turbid to clear plaque formers among phage survivors. the findings suggested that the uv-ind ...19873574328
prosthetic valve endocarditis caused by acinetobacter calcoaceticus subsp. lwoffi.acinetobacter spp. are uncommon etiologic agents of prosthetic valve endocarditis. two patients with acinetobacter calcoaceticus subsp. lwoffi prosthetic valve endocarditis are described. the patients were successfully treated with antibiotics (cefotaxime sodium and gentamicin sulfate); thus, we suggest medical treatment rather than early valve replacement in this particular type of infection.19873584434
comparative activity in vitro of ticarcillin and ticarcillin plus clavulanic acid against enterobacteriaceae, pseudomonas aeruginosa and acinetobacter calcoaceticus. 19873151358
benzoate and muconate, structurally dissimilar metabolites, induce expression of cata in acinetobacter calcoaceticus.biosynthetic regulation of cata, the gene encoding catechol 1,2-dioxygenase (ec, was studied in an acinetobacter calcoaceticus mutant strain unable to metabolize benzoate. benzoate and muconate independently induced the enzyme. in glucose-grown cells, benzoate yielded higher enzyme levels than did muconate, whereas muconate was the more effective inducer in succinate-grown cells.19873793718
evaluation of enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis in genital tract enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis was evaluated on genital specimens from 96 men and 272 women attending a clinic for sexually transmitted diseases (std clinic). compared with a direct immunofluorescence test for chlamydial elementary bodies, the enzyme immunoassay had a sensitivity of 58% on specimens from men, a specificity of 90%, a positive predictive value of 93%, and a negative predictive value of 88%; the assay had a sensitivity of 67% on specimens ...19873546397
the epidemiology of acinetobacter calcoaceticus: biotype and resistance-pattern of 328 strains consecutively isolated from clinical specimens.328 clinical isolates of acinetobacter calcoaceticus from hospitalized patients and persons attending general practitioners were characterized according to biotype and resistance-pattern. 117 strains of biotype (b.) anitratus, 200 of b.lwoffi, 11 of b. haemolyticus and no strains of b. alcaligenes were found. b. anitratus was more frequently isolated from patients in hospitals than b.lwoffi; b.lwoffi was more often found in general practice. strains of b. anitratus had more resistance traits tha ...19873565019
reconstitution of emulsifying activity of acinetobacter calcoaceticus bd4 emulsan by using pure polysaccharide and protein.acinetobacter calcoaceticus bd4 and bd413 produce extracellular emulsifying agents when grown on 2% ethanol medium. for emulsifying activity, both polysaccharide and protein fractions were required, as demonstrated by selective digestion of the polysaccharide with a specific bacteriophage-borne polysaccharide depolymerase, deproteinization of the extracellular emulsifying complex with hot phenol, and reconstitution of emulsifier activity with pure polysaccharide and a polysaccharide-free protein ...19873566271
[kinetics of membrane-bound aldehyde dehydrogenase from acinetobacter calcoaceticus].in recent investigations we were able to demonstrate that the nadp-dependent aldehyde dehydrogenase of acinetobacter calcoaceticus is an inducible enzyme localized in intracytoplasmic membranes limiting alkane inclusions. long-chain aliphatic hydrocarbons and alkanols are inducers of the enzyme. it was purified by us and now kinetically characterized using the enzyme-micelle form, which contains bacterial phospholipids and a detergent (sodium cholate), too. the ph optimum of aldehyde dehydrogena ...19873566912
defensins mediate the microbicidal activity of human neutrophil granule extract against acinetobacter acid extract of human neutrophil granules was fractionated on a sephadex g-100 column and tested for microbicidal activity against acinetobacter calcoaceticus ho-1 as described previously (m.c. modrzakowski and c. m. paranavitana, infect. immun. 32:668-674, 1981). the low-molecular-weight protein fraction, peak d, accounted for about 30% of the protein and nearly all of the activity of the crude extract against strain ho-1. peak d protein proved to be a mixture of the three human defensin pep ...19873570467
[effect of the slime from acinetobacter calcoaceticus on the function of phagocytic cells]. 19873127500
dienelactone hydrolase from pseudomonas sp. strain b13.dienelactone hydrolase (ec catalyzes the conversion of cis- or trans-4-carboxymethylenebut-2-en-4-olide (dienelactone) to maleylacetate. an approximately 24-fold purification from extracts of 3-chlorobenzoate-grown pseudomonas sp. strain b13 yielded a homogeneous preparation of the enzyme. the purified enzyme crystallized readily and proved to be a monomer with a molecular weight of about 30,000. each dienelactone hydrolase molecule contains two cysteinyl side chains. one of these was ...19873804973
cell envelope protein profiles of acinetobacter calcoaceticus strains isolated in hospitals.the cell envelope protein patterns of 78 strains of acinetobacter calcoaceticus, mainly isolated in hospitals, were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). the patterns were stable and reproducible. comparison of the protein profiles made possible differentiation between two groups of strains. the patterns of the first group could be classified on the basis of concordance. some profiles appeared to be associated with the epidemiological origin of the st ...19873585964
a simple spectrophotometric assay for arogenate dehydratase.a simple spectrophotometric assay for arogenate dehydratase, the enzyme that catalyzes the formation of l-phenylalanine from l-arogenate, is presented. the method couples the arogenate dehydratase reaction with that of an aromatic aminotransferase partially purified from acinetobacter calcoaceticus. in the presence of 2-ketoglutarate, phenylpyruvate formation is measured at 320 nm at basic ph. the method was compared with two other methods already in use in our laboratory for arogenate dehydrata ...19873619011
chronic acinetobacter calcoaceticus var anitratus pneumonia.gram-negative bacteria most often affect the lung in an acute, suppurative process; however, these organisms may also produce chronic pneumonia. acinetobacter calcoaceticus has not been reported previously as a cause of chronic pneumonia. we present a patient with fatal, chronic community-acquired acinetobacter pneumonia with chest wall invasion at autopsy.19873631123
acinetobacter calcoaceticus infection following a dog bite.a frequent complication of dog bite wounds is bacterial infection. the choice of antibiotics is based upon the most likely organisms. failure to achieve wound healing suggests that an uncommon organism(s) is present and should lead the clinician to culture the wound. a case of acinetobacter calcoaceticus infection following a dog bite is described.19873668199
drag reduction by acinetobacter calcoaceticus bd4.the encapsulated bacterium acinetobacter calcoaceticus bd4 at a density of 3.6 x 10(9) cells per ml reduced the friction of turbulent water in a narrow pipe by 55%. this drag reduction was due to the tightly bound polysaccharide capsules (0.4 mg per ml) of culture. capsule-deficient mutants of bd4 failed to reduce drag. the cell-bound polysaccharide demonstrated a threefold-higher drag-reducing activity than the polymer which was free in solution.19873674875
isolation and identification of a rhamnosyl-rhamnosyl-3-deoxy-d-manno-octulosonic acid trisaccharide from the lipopolysaccharide of acinetobacter calcoaceticus (10303 ntcc london). 19873690574
epidemiology of nosocomial infections due to acinetobacter calcoaceticus. 19872889762
biotyping of acinetobacter calcoaceticus using the api 2one system.the api 2one system for the identification of non-fermentative gram-negative bacilli enables the discrimination of a possible 209 different biotypes of acinetobacter spp. and consequently has potential for use as an acinetobacter spp. typing system. a total of 122 separate strains of acinetobacter spp. isolated in nottingham hospitals over a 4 year period from a wide variety of clinical specimens, divided into 31 different biotypes which were stable over a 1 year storage period. two biotypes pre ...19872889767
endemic occurrence of acinetobacter calcoaceticus biovar anitratus in an intensive care strain of acinetobacter calcoaceticus biovar anitratus caused colonization of 111 patients admitted to an intensive care unit (icu) during a 2-year period. all patients were intubated and had received antibiotic therapy prior to colonization. morbidity due to the organism was about 1%. the colonization rate showed a decreasing trend during the study period, but no seasonal variation. the strain was found in the air in a low concentration and on the hands of 8-13% of the members of the staff. ...19872891755
involvement of a plasmid in growth on and dispersion of crude oil by acinetobacter calcoaceticus ra57.a crude-oil-degrading acinetobacter species, acinetobacter calcoaceticus ra57, was isolated by standard enrichment culture techniques on the basis of its ability to utilize the oily sludge found in the vicinity of a local gas station. strain ra57 was found to contain four plasmids: psr1 (5.1 kilobases [kb]), psr2 (5.4 kb), psr3 (10.5 kb), and psr4 (20 kb). both supercoiled and open circular forms of the first three plasmids were identified by two-dimensional gel electrophoresis. restriction endo ...19872821903
cloning and expression in escherichia coli of acinetobacter calcoaceticus genes for benzoate degradation.the catabolic genes necessary for the conversion of benzoate to catechol have been cloned from acinetobacter calcoaceticus into escherichia coli. the cloned genes, benabcd, encoded both a benzoate 1,2-dioxygenase system, composed of nadh-cytochrome c reductase and terminal oxygenase components, and a cis-diol dehydrogenase. the dioxygenase system appears to be encoded by three genes, benabc, whose products, 53-, 19-, and 38-kilodalton proteins, correspond in size to those of components in other ...19872824437
[alpha-aminomethylketones as inhibitors of a membrane-bound alanine aminopeptidase].methyl ketone derivatives of l-amino acids are substrate analogous inhibitors of membrane bound alanine aminopeptidase (ec from acinetobacter calcoaceticus. the type of inhibition is competitive. compounds with a branched aliphatic side chain are most effective so that valine methyl ketone was found to be the best inhibitor of the enzyme with a k-value of 5.5.10(-7) mol.l-1.19872895641
cloning and genetic organization of the pca gene cluster from acinetobacter calcoaceticus.the beta-ketoadipate pathway of acinetobacter calcoaceticus comprises two parallel metabolic branches. one branch, mediated by six enzymes encoded by the cat genes, converts catechol to succinate and acetyl coenzyme a (acetyl-coa); the other branch, catalyzed by products of the pca genes, converts protocatechuate to succinate and acetyl-coa by six metabolic reactions analogous or identical to those of the catechol sequence. we used the expression plasmid puc18 to construct expression libraries o ...19873036773
outer membrane permeability of acinetobacter calcoaceticus mediates susceptibility to rat polymorphonuclear leukocyte granule contents.growth of acinetobacter calcoaceticus on specific alkanes altered the outer membrane permeability of the organism, as indicated by a change in sensitivity to the antibiotic actinomycin d. as the carbon length of the alkane energy source decreased, outer membrane permeability and susceptibility to actinomycin d increased. concomitant with the increase in outer membrane permeability, a. calcoaceticus became more susceptible to the oxygen-independent antimicrobial activity of extracted contents fro ...19873040593
bacterial nad(p)-independent quinate dehydrogenase is a quinoprotein.acinetobacter calcoaceticus lmd 79.41 produced significant amounts of pyrrolo-quinoline quinone (pqq) in its culture medium when grown on quinic acid or shikimic acid. studies with lmd 79.41 and pqq- -mutants of this strain demonstrated that this organism contains an nad(p)-independent quinate dehydrogenase (qdh) (ec 1.1.99.-), catalyzing the first degradation step of these compounds, and that the enzyme contains pqq as a cofactor, i.e. is a quinoprotein. synthesis of qdh was induced by protocat ...19883044290
chorismate mutase:prephenate dehydratase from acinetobacter calcoaceticus. purification, properties and immunological cross-reactivity.the bifunctional p protein (chorismate mutase: prephenate dehydratase) from acinetobacter calcoaceticus has been purified. it was homogeneous in polyacrylamide gels and was more than 95% pure on the basis of the immunostaining of purified p protein with the antibodies raised against the p protein. the native enzyme is a homodimer (mr = 91,000) composed of 45-kda subunits. a twofold increase in the native molecular mass of the p protein occurred in the presence of l-phenylalanine (inhibitor of bo ...19883046943
a belgian multicentre in-vitro study of a large multicentre study, the susceptibility of 2171 bacterial isolates to ofloxacin and other antibiotics has been examined by the kirby-bauer method. susceptibility to ofloxacin (breakpoint less than or equal to 2 mg/l) was observed in 99.2% of 1104 strains enterobacteriaceae, 88.9% of 217 pseudomonas aeruginosa, 93.6% of 31 other pseudomonas spp., 96.3% of 27 acinetobacter calcoaceticus, 99.2% of 118 haemophilus influenzae, 97.4% of 423 staphylococci, 79.4% of 107 streptococcus faecalis a ...19883053578
comparison of mandelate dehydrogenases from various strains of acinetobacter calcoaceticus: similarity of natural and 'evolved' previous work it had been shown that acinetobacter calcoaceticus wild-type strain ncib 8250 had only an l-mandelate deydrogenase but it could give rise to mutants that contained an evolved d-mandelate dehydrogenase; conversely, wild-type strain ebf 65/65 had only a d-mandelate dehydrogenase but gave rise to mutants that possessed an evolved l-mandelate dehydrogenase. several other wild-type strains of a. calcoaceticus have now been shown to grow on both enantiomers of mandelate. in every case ...19883053984
benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus. substrate specificities and inhibition studies.the apparent km and maximum velocity values of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus were determined for a range of alcohols and aldehydes and the corresponding turnover numbers and specificity constants were calculated. benzyl alcohol was the most effective alcohol substrate for benzyl alcohol dehydrogenase. perillyl alcohol was the second most effective substrate, and was the only non-aromatic alcohol oxidized. the other substrates of b ...19883060114
flow cytometric screening and isolation of escherichia coli clones which express surface antigens of the oil-degrading microorganism acinetobacter calcoaceticus rag-1.flow cytometry (fcm) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of escherichia coli clones containing a genomic library from the oil-degrading microorganism acinetobacter calcoaceticus rag-1 surface antigens. reconstruction experiments using mixed populations indicated that rag-1 cells could be clearly distinguished at a ratio of one rag-1 cell to 500 escherichia coli cells. using this technique two clones, wm143 and wm191, were isolated and shown ...19883061370
acinetobacter calcoaceticus bacteremia 1984-1987. 19883068198
comparative in vitro activity of lomefloxacin and other antimicrobials against 597 microorganisms causing bacteremia.the in vitro activity of lomefloxacin, a new fluorinated quinolone antimicrobial, was compared to that of enoxacin, norfloxacin, ofloxacin, imipenem, cefotaxime, ceftazidime, and tobramycin against 597 microorganisms isolated from bacteremic patients at a university hospital. overall, lomefloxacin had activity similar to that of enoxacin and norfloxacin but less than that of ofloxacin. lomefloxacin had excellent activity against members of the family enterobacteriaceae and haemophilus influenzae ...19883071448
cloning and expression of pca genes from pseudomonas putida in escherichia coli.beta-ketoadipate elicits expression of five structural pca genes encoding enzymes that catalyse consecutive reactions in the utilization of protocatechuate by pseudomonas putida. three derivatives of p. putida prs2000 were obtained, each carrying a single copy of tn5 dna inserted into a separate region of the genome and preventing expression of different sets of pca genes. selection of tn5 in or near the pca genes in these derivatives was used to clone four structural pca genes and to enable the ...19883076176
antibiogram, biotyping and plasmid profile analysis of acinetobacter calcoaceticus var. anitratus.antibiogram, biotyping, and plasmid profile analysis were studied on 115 isolates (40 nosocomial and 75 community strains) of acinetobacter calcoaceticus var. anitratus isolated from clinical specimens. by disk diffusion method, we found 14 antimicrobial susceptibility patterns. among them, the gentamicin-resistant patterns were recovered more frequently from nosocomial origin than from community-acquired origin. fifteen patterns of biocodes were found by using gni card of automicrobic system (v ...19883076859
hand disinfection: a comparison of various agents in laboratory and ward studies.the efficacy of 14 handwashing or disinfectant preparations was compared in laboratory tests on staff volunteers. the test organism, escherichia coli, was applied to the fingertips and log reductions (lr) were measured following treatment with the test agent and control preparations (70% isopropanol and non-medicated bar soap). alcoholic preparations, particularly n-propanol and isopropanol were the most effective showing lrs of 3.1-3.8. chlorhexidine (lr 2.9) and povidone-iodine detergent prepa ...19882899107
endemic occurrence of acinetobacter calcoaceticus biovar anitratus in an intensive care unit. 19882905720
a study of the value of electrophoretic and other techniques for typing acinetobacter calcoaceticus.forty-four isolates of acinetobacter calcoaceticus var anitratus collected during hospital outbreaks were studied using polyacrylamide gel electrophoresis (page), plasmid analysis, antibiograms and biochemical tests to determine their degree of similarity. reproducibility tests were also carried out on the page and biochemical techniques to determine their validity when used to compare bacteria of the same type isolated intermittently. page data was analysed densitometrically and isolates compar ...19882907333
virulence genes, borders, and overdrive generate single-stranded t-dna molecules from the a6 ti plasmid of agrobacterium tumefaciens.agrobacterium tumefaciens transfers the t-dna portion of its ti plasmid to the nuclear genome of plant cells. upon cocultivation of a. tumefaciens a348 with regenerating tobacco leaf protoplasts, six distinct single-stranded t-dna molecules (t strands) were generated in addition to double-stranded t-dna border cleavages which we have previously reported (k. veluthambi, r.k. jayaswal, and s.b. gelvin, proc. natl. acad. sci. usa 84:1881-1885, 1987). the t region of an octopine-type ti plasmid has ...19882832367
cloning of the gene encoding quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus: evidence for the presence of a second enzyme.we cloned the gene coding for the quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. this clone complements gdh mutations in a. calcoaceticus, pseudomonas aeruginosa, and escherichia coli. the gene codes for a protein with an mr of 83,000. evidence is presented for the presence of two different glucose dehydrogenase enzymes in a. calcoaceticus: a protein with an mr of 83,000 and a dimer of two identical subunits with an mr of 50,000.19882834325
cosmid cloning of five zymomonas trp genes by complementation of escherichia coli and pseudomonas putida trp mutants.a library of zymomonas mobilis genomic dna was constructed in the broad-host-range cosmid plafr1. the library was mobilized into a variety of escherichia coli and pseudomonas putida trp mutants by using the helper plasmid prk2013. five z. mobilis trp genes were identified by the ability to complement the trp mutants. the trpf, trpb, and trpa genes were on one cosmid, while the trpd and trpc genes were on two separate cosmids. the organization of the z. mobilis trp genes seems to be similar to th ...19882838460
inactivation of acinetobacter calcoaceticus acetate kinase by diethylpyrocarbonate.acetate kinase purified from acinetobacter calcoaceticus was inhibited by diethylpyrocarbonate with a second-order rate constant of 620 m-1.min-1 at ph 7.4 at 30 degrees c and showed a concomitant increase in absorbance at 240 nm due to the formation of n-carbethoxyhistidyl derivative. activity could be restored by hydroxylamine and the ph curve of inactivation indicates the involvement of a residue with a pka of 6.64. complete inactivation of acetate kinase required the modification of seven re ...19882844264
a search for intermediates in the bacterial biosynthesis of pqq.studies on the biosynthesis of pyrroloquinoline quinone (pqq) were performed with acinetobacter calcoaceticus pqq- -mutants belonging to genetically different complementation groups. all mutants were unable to grow on l-arabinose, the conversion of this substrate by the organism only occurring via membrane-bound quinoprotein (pqq-containing) glucose dehydrogenase. in general, the same observation and conclusion applied to shikimate and quinate, requiring active quinoprotein quinate dehydrogenase ...19882855587
acinetobacter calcoaceticus foot infection secondary to high-pressure injection injury: a case report.injection injuries are surgical emergencies occurring most often in the hand and frequently associated with widespread tissue necrosis and infection. this report presents a case of high-pressure injection injury of the foot associated with extensor hallucis longus laceration and infection with acinetobacter calcoaceticus variant anitratus. this injury occurred with a high-pressure "water-blaster" tool used to remove paint from pavement. similar injuries in the foot have not been reported. a. cal ...19882895055
antimicrobial mechanisms against acinetobacter calcoaceticus of rat polymorphonuclear leukocyte granule extract.the antimicrobial mechanisms of rat polymorphonuclear leukocyte granule extract and isolated extract fractions against acinetobacter calcoaceticus were examined. crude granule extract and a fraction containing low-molecular-weight cationic peptides (peak d) reduced the viability of a. calcoaceticus and inhibited the uptake of radiolabeled macromolecule precursors by cells. the inhibitory activity observed with peak d was not as great as that of crude granule extract containing equivalent amounts ...19882449397
in vitro studies of fleroxacin (ro 23-6240), a new trifluorinated quinolone derivative.the in vitro activity of fleroxacin (ro 23-6240) against 441 bacterial isolates was compared with those of ciprofloxacin, ofloxacin, amoxycillin, cefadroxil, cefuroxime and tobramycin. an agar dilution method was used for the determination of minimal inhibitory concentrations (mics). ciprofloxacin showed the highest activity against the enterobacteriaceae, 95% of the isolates were inhibited by 0.06 mg/l, but fleroxacin and ofloxacin were also highly active (mic 90% = 0.5 and 0.25 mg/l, respectiv ...19883141116
endemic acinetobacter anitratus in a surgical intensive care unit: mechanical ventilators as reservoir.colonization and infection with an endemic multiresistant strain of acinetobacter calcoaceticus variety anitratus had been observed in the surgical intensive care unit of a university hospital since 1982. an outbreak of infection with this endemic, multiresistant acinetobacter anitratus strain occurred between january and september, 1984. after initial attempts at identification of environmental reservoirs had been unsuccessful, risk factors for the acquisition of acinetobacter anitratus were in ...19883141154
[in vitro antibacterial activity of cefpirome in combination with 4 aminoglycosides and 2 fluoroquinolones].combinations of cefpirome with aminoglycosides (gentamicin, tobramycin, netilmicin, amikacin) and of cefpirome with fluorinated quinolones (pefloxacin, ofloxacin) were tested against 20 enterobacteriaceae, 6 pseudomonas aeruginosa, 6 acinetobacter calcoaceticus, 6 group d streptococci and 6 staphylococcus aureus strains, susceptible, intermediate or resistant with a low level to these different compounds. activity of combinations was evaluated using a broth microdilution checkerboard method and ...19883141888
[serological typing of acinetobacter calcoaceticus subspecies anitratus strains by soft-agar technique]. 19883146608
leukopenic and lethal effects of slime from acinetobacter calcoaceticus. 19883150412
susceptibility of 310 nonfermentative gram-negative bacteria to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin.the susceptibility of 310 mainly clinically isolated gram-negative nonfermenters to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin was determined by agar dilution. all 3 quinolones were very active, with mic90 ranges (micrograms/ml) against all organisms ranging between 0.25 and 8 for ciprofloxacin, and 0.25 and 16.0 for ofloxacin and fleroxacin, respectively. aztreonam and carumonam showed less activity than the quinolones on a weight-for-weight basis, with mic90 values ranging f ...19883127127
[acinetobacter calcoaceticus var. iwoffi as the cause of abscess formation in han:nmri nude mice]. 19882973310
[spontaneous bacterial peritonitis caused by acinetobacter calcoaceticus. a rare nosocomial infection in the cirrhotic patient]. 19882974144
[susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates].susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ...19883163383
dna sequence of the acinetobacter calcoaceticus catechol 1,2-dioxygenase i structural gene cata: evidence for evolutionary divergence of intradiol dioxygenases by acquisition of dna sequence repetitions.the dna sequence of a 1.6-kilobase-pair sali-kpni acinetobacter calcoaceticus restriction fragment carrying cata, the structural gene for catechol 1,2-dioxygenase i, was determined. the 933-nucleotide gene encodes a protein product with a deduced molecular weight of 34,351. the similarly sized pseudomonas clca gene encodes catechol 1,2-dioxygenase ii, an enzyme with relatively broad substrate specificity and relatively low catalytic efficiency. comparison of the cata and clca sequences demonstra ...19883170486
[susceptibility of gram-negative bacterial isolates to six beta-lactam and two aminoglycoside antibiotics at our university hospital].susceptibilities of gram-negative bacterial strains isolated at the university hospital in 1987 to six beta-lactam antibiotics (azthreonam cefmetazole, ceftizoxime, latamoxef, aminobenzyl-penicillin and piperacillin) and two aminoglycoside antibiotics (gentamicin and amikacin) were examined by agar dilution and the agar diffusion method. results obtained from both methods correlated well in most of the strains. most of the strains belonging to escherichia coli, klebsiella oxytoca, klebsiella pne ...19883175388
cytochrome b-562 from acinetobacter calcoaceticus l.m.d. 79.41. its characteristics and role as electron acceptor for quinoprotein glucose dehydrogenase.a soluble cytochrome b was purified from acinetobacter calcoaceticus l.m.d. 79.41. on the basis of the alpha-band maximum of a reduced preparation, measured at 25 degrees c, it is designated as cytochrome b-562. this cytochrome is a basic monomeric protein (pi 10.2; mr 18,000), containing one protohaem group per molecule. the reduced form, at 25 degrees c, showed absorption bands at 428, 532 and 562 nm. at 77 k the alpha-band shifted to 560 nm (with a shoulder at 558 nm). the reduced cytochrome ...19883178744
synergistic interactions of ciprofloxacin and extended spectrum beta-lactams or aminoglycosides against acinetobacter calcoaceticus ss. anitratus.the susceptibility of 54 clinical isolates of acinetobacter calcoaceticus ss. anitratus to 16 antimicrobial agents was determined in vitro with inoculum of 10(4) and 10(6) cfu by a standard agar dilution method. the most active agents were imipenem, sch 34343, ciprofloxacin, difloxacin (a-56619), and a-56620. only imipenem and abbott quinolones (a-56619 and a-56620) remained active when tested with the heavier inoculum. except for ticarcillin and ceftazidime, which showed only moderate activity, ...19883180706
in-vitro and in-vivo activities of t-3262, a new pyridone carboxylic acid.t-3262[p-toluenesulfonic acid salt of dl-7-(3-amino-1-pyrrolidinyl)-1-(2,4-difluorophenyl)-6-fluoro-1, 4-dihydro-4-oxo-1, 8-naphthyridine-3-carboxylic acid monohydrate] is a new pyridone carboxylic acid with a broad spectrum of antibacterial activity against gram-positive and gram-negative bacteria. the activity of t-3262 against most enterobacteriaceae was comparable with that of ciprofloxacin except proteus spp. and providencia rettgeri and exceeded that of ofloxacin and norfloxacin. the activ ...19883182417
multiple intensive care unit outbreak of acinetobacter calcoaceticus subspecies anitratus respiratory infection and colonization associated with contaminated, reusable ventilator circuits and resuscitation bags.acinetobacter calcoaceticus subspecies anitratus (a. anitratus) can cause nosocomially and community acquired pneumonia. source identification of the organism is often difficult. an outbreak of respiratory infection and colonization with a. anitratus affecting 93 ventilated patients in all six of a hospital's intensive care units (icus) over 10 months is described.19883189366
isolation of acinetobacter calcoaceticus from septicaemic hens. 19883195030
taxonomic studies of acinetobacter species based on the electrophoretic analysis of enzymes.thirty six strains of acinetobacter, 22 strains from the culture collections and 14 isolates from soil and cotton samples, were examined for the presence of twelve enzymes using polyacrylamide gel electrophoresis. a numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. the strains used were divided into four clusters (z-1, z-2, z-3 and z-4), and cluster z-1 was further divided into three subclusters (z-1a, z-1b and z-1c) ...19883210151
[characteristics of acinetobacter sp. strains isolated from a hospital environment. iii. anitracinogenic properties of acinetobacter calcoaceticus isolated from clinical specimens and other sources]. 19883210835
[utilization of 3-chlorobenzoic acid by a mixed culture of microorganisms].a mixed microbial culture (acinetobacter calcoaceticus inmi-kz-3 and alcaligenes faecalis inmi-kz-5) completely utilises 3-chlorobenzoic acid (3-cba), and the liberation of chlorine atoms is 100% of the theoretically possible one. a. faecalis growth in the mixed culture at the account of 2-chloro-cis,cis-muconic acid which is accumulated in the course of 3-cba metabolism by a. calcoaceticus inmi-kz-3.19883211007
community-acquired bacteremic acinetobacter calcoaceticus var. anitratus pneumonia. 19883215692
[acinetobacter calcoaceticus: epidemiologic aspects and distribution in the natural and hospital environment]. 19883226936
[antibiotic sensitivity of nonfermenting gram-negative bacteria].antibiotic resistance of 132 strains of nonfermenting gram-negative bacteria (ngnb) was studied. 43, 20, 17, 14 and 12 of them belonged to acinetobacter calcoaceticus (anitratus and lwoffi), pseudomonas cepacia, alcaligenes faecalis, p. stutzeri and p. maltophilia, respectively. with rare exceptions all the strains were resistant to benzylpenicillin, oxacillin, lincomycin, ampicillin and cephaloridine. sensitivity to the other antibiotics varied within wide ranges. amikacin (94.3 per cent) and t ...19883228322
antimicrobial activity of mdl 19,592: an oral cephalosporin.mdl 19,592, a semisynthetic oral cephalosporin, has an antimicrobial spectrum principally directed against gram-positive cocci (mic50, 0.25-4 micrograms/ml), branhamella catarrhalis (mic50, 1-2 micrograms/ml), and pathogenic neisseria spp. such as meningococci (mic50, 4 micrograms/ml). enterobacteriaceae, enterococci, pseudomonas spp., corynebacterium jeikeium, and acinetobacter calcoaceticus strains had mdl 19,592 mic50 of greater than or equal to 32 micrograms/ml. although mdl 19,592 generally ...19883246143
effect of hexadecane-induced vesiculation on the outer membrane of acinetobacter calcoaceticus.lipopolysaccharide-rich vesicles were released from acinetobacter calcoaceticus 69v during growth on hexadecane. vesicle formation occurred over the whole surface of the cell as demonstrated by scanning electron microscopy. in contrast, the surface of acetate-grown cells, for which little lipopolysaccharide was found in the growth medium, appeared smooth. the overall chemical composition as well as the protein and phospholipid composition of the outer membranes of both cell types was very simila ...19883246592
[hemolytic properties of acinetobacter calcoaceticus strains]. 19883246922
[the synthesis of group b vitamins under various conditions for the cultivation of acinetobacter calcoaceticus on ethanol media]. 19883249582
occurrence, transfer and mobilization in epilithic strains of acinetobacter of mercury-resistance plasmids capable of transformation.a 7.8 kb plasmid (pqm17) encoding mercury resistance was isolated from two epilithic strains of acinetobacter calcoaceticus. the plasmid had a broad host range when mobilized by rp1, transferring into pseudomonas aeruginosa, p. putida, p. fluorescens, escherichia coli, proteus vulgaris and chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. the plasmid could be transferred into a. calcoaceticus bd413 using intact cells of donor and recipient bacteria (i. ...19883254940
distribution of beta-lactamases and phenotype analysis in clinical strains of acinetobacter hundred clinical strains of acinetobacter calcoaceticus isolated from 1981 to 1986 were screened for enzymatic resistance to beta-lactam antibiotics. fourteen beta-lactam antibiotics were tested and four phenotypes were defined on the basis of enzymatic resistance and of susceptibility to the following beta-lactams: ticarcillin, piperacillin, cefotaxime, and ceftazidime. the resistance of a. calcoaceticus to beta-lactam antibiotics was predominantly due to beta-lactamases, which were produce ...19883264830
benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus. purification and preliminary characterization.a quick, reliable, purification procedure was developed for purifying both benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from a single batch of acinetobacter calcoaceticus n.c.i.b. 8250. the procedure involved disruption of the bacteria in the french pressure cell and preparation of a high-speed supernatant, followed by chromatography on deae-sephacel, affinity chromatography on blue sepharose cl-6b and matrex gel red a, and finally gel filtration through a superose 12 fast-prot ...19883291854
production of a phosphatidylglycerol-like substance by genital flora bacteria.bacteria contaminating amniotic fluid have anecdotally been shown to produce a phosphatidylglycerol-like substance, thus giving false positive tests for phosphatidylglycerol in vaginal pool fluid from patients with premature rupture of the membranes. isolates of escherichia coli, proteus mirabilis, acinetobacter calcoaceticus, klebsiella pneumoniae, staphylococcus aureus, coagulase-negative staphylococcus, streptococcus faecalis, and streptococcus agalactiae (group b streptococcus) were grown in ...19883293449
comparative in vitro activity of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone against hospital strains of gram-negative and -positive bacteria.the in vitro antibacterial activities of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone were compared against 330 gram-negative and gram-positive strains isolated from clinical specimens received at the king abdulaziz university hospital (kauh) in saudi arabia. the antibacterial susceptibility was determinated by stokes method and by the minimal inhibitory concentration (mic) using an agar dilution method. ceftazidime and ceftriaxone were the most active antibiotics, inhibiting ...19883293818
criteria for disk susceptibility tests and quality control guidelines for the cefoperazone-sulbactam combination.for in vitro susceptibility tests with cefoperazone and sulbactam (a beta-lactamase inhibitor), 75/30-micrograms disks may be used with the interpretive zone size breakpoints that are currently used for 75-micrograms cefoperazone disks. for dilution tests, a 2:1 ratio of cefoperazone to sulbactam is recommended. for quality control purposes, mic limits that are used to monitor cefoperazone tests were also applied to tests with the combination of drugs. for gram-negative control strains, zone siz ...19883343304
community-acquired acinetobacter pneumonia in adults in papua new guinea.acinetobacter calcoaceticus, an aerobic gram-negative coccobacillus, is a rare cause of community-acquired pneumonia. it most commonly causes nosocomial infections, particularly in elderly debilitated patients who have undergone surgery, instrumentation, and antibiotic therapy. in a study of acute pneumonia in adults, five cases of community-acquired acinetobacter pneumonia were observed over an 11-month period. other than chronic pulmonary disease (two patients), no serious underlying diseases ...19883393786
nucleotide sequence of the gene coding for quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. 19883399393
nosocomial respiratory tract colonization and infection with aminoglycoside-resistant acinetobacter calcoaceticus var anitratus: epidemiologic characteristics and clinical significance.during the period july 1983 through december 1984, aminoglycoside-resistant acinetobacter calcoaceticus var anitratus (aca) were isolated from 98 patients in a university hospital. eighty-seven percent of patients (85/98) acquired aminoglycoside-resistant aca in the intensive care unit (icu) and 92% (90/98) of all initial isolates were from sputum. icu patients with respiratory colonization/infection with aminoglycoside-resistant aca were compared with matched icu controls with other gram-negati ...19883403939
production of biodispersan by acinetobacter calcoaceticus a2.bacterial strains a2 and he5, isolated by enrichment culture techniques, were shown to produce extracellular, nondialyzable materials which disperse limestone powders in water. these materials are referred to as biodispersans. strains a2 and he5 were classified as acinetobacter calcoaceticus by physiological and genetic tests. an assay for limestone-dispersing activity was developed which is based on the settling time of a standard 10% limestone-in-water mixture. the assay was proportional to bi ...198816347544
purification and chemical properties of acinetobacter calcoaceticus a2 biodispersan.the extracellular dispersant of acinetobacter calcoaceticus a2, referred to as biodispersan, was concentrated by ammonium sulfate precipitation and deproteinized by hot phenol treatment. the active component was an anionic polysaccharide (ps-a2). the specific activity of ps-a2 was approximately three times greater than that of crude biodispersan. ps-a2 had a sedimentation constant of 1.39 s, a diffusion coefficient of 18.8 x 10 cm s, and a partial molar volume of 0.65 cm g, yielding an average m ...198816347545
bacterial desorption from food container and food processing surfaces.the desorption ofstaphylococcus aureus, acinetobacter calcoaceticus, and a coryneform from the surfaces of materials used for manufacturing food containers (glass, tin plate, and polypropylene) or postprocess canning factory conveyor belts (stainless steel and nylon) was investigated. the effect of time, ph, temperature, and adsorbed organic layers on desorption was studied.s. aureus did not detach from the substrata at any ph investigated (between ph 5 and 9).a. calcoaceticus and the coryneform ...198824203003
effect of emulsan on biodegradation of crude oil by pure and mixed bacterial cultures.crude oil was treated with purified emulsan, the heteropolysaccharide bioemulsifier produced by acinetobacter calcoaceticus rag-1. a mixed bacterial population as well as nine different pure cultures isolated from various sources was tested for biodegradation of emulsan-treated and untreated crude oil. biodegradation was measured both quantitatively and qualitatively. recovery of co(2) from mineralized c-labeled substrates yielded quantitative data on degradation of specific compounds, and capil ...198916347832
biodegradation of asphalt cement-20 by aerobic gram-negative, aerobic bacteria were isolated from a mixed culture enriched for asphalt-degrading bacteria. the predominant genera of these isolates were pseudomonas, acinetobacter, alcaligenes, flavimonas, and flavobacterium. the mixed culture preferentially degraded the saturate and naphthene aromatic fractions of asphalt cement-20. a residue remained on the surface which was resistant to biodegradation and protected the underlying asphalt from biodegradation. the most potent asphalt-deg ...198916347928
cell adsorption and local accumulation of extracellular polysaccharide in an immobilized acinetobacter calcoaceticus system.acinetobacter calcoaceticus can be immobilized on celite by adsorption. the salt concentrations suitable for immobilized cell fermentation are between 10 and 50 mm phosphate concentration. low salt concentrations cause desorption of immobilized cells while high salt concentrations inhibit the adsorption of cells on celite. it is also found that cell adsorption is better at lower ph than at higher ph. an airlift fermentation using immobilized cells at 300 g/l celite loading shows that about 70% o ...198918588066
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