TitleAbstractYear(sorted descending)
beta-ketoadipate enol-lactone hydrolases i and ii from acinetobacter calcoaceticus.beta-ketoadipate enol-lactone hydrolase catalyzes a common step in the utilization of protocatechuate and cis,cis-muconate by bacteria. either of the two compounds elicits the synthesize of an enol-lactone hydrolase in acinetobacter. the enol-lactone hydrolase that is induced by each compound was purified, and the properties of the proteins were compared. both enzymes appear to be dimers with molecular weights of approximately 25,000. the amino acid compositions of the enzymes differ, and the tw ...19751158871
metabolism of monofluorobenzoates by acinetobacter calcoaceticus n.c.i.b. 8250. formation of monofluorocatechols.none of the monofluorobenzoates serves as sole source of carbon and energy for growth of acinetobacter calcoaceticus but all can contribute to growth on other substrates. the monofluorobenzoates are oxidised by bacteria pre-induced for benzoate oxidation and can themselves induce the appropriate enzymes. the initial products of oxidation have been separated and identified by gas-liquid chromatography. 2-flurobenzoate is oxidised to catechol, fluoride and 3-fluorocatechol; 3-fluorobenzoate gives ...19751182155
[inhibiton of isocitrate dehydrogenase and isocitrate lyase from acinetobacter calcoaceticus by acids of the citrate and glyoxylate cycle].acinetobacter calcoaceticus contains two forms of nadp+-dependent isocitrate dehydrogenases differing, among others, by their molecular weights and regulatory properties. the regulation of the high-molecular form of isocitrate dehydrogenase and of isocitrate lyase by organic acids, either belonging or related to the citrate and glyoxalate cycle, is investigated. while alpha-ketoglutarate and oxalacetate competitively inhibit the isocitrate dehydrogenase against ds-isocitrate, glyoxylate and pyru ...19751199592
regulation of growth of acinetobacter calcoaceticus ncib8250 on l-mandelate in batch culture.batch culture of acinetobacter calcoaceticus in l-mandelate- or phenylglyoxylate-salts medium showed an unusual non-exponential pattern unless the inoculum had been grown on benzyl alcohol. there were transient accumulations of benzaldehyde and benzyl alcohol caused by the limitation of l-mandelate oxidation by low activities of benzaldehyde dehydrogenase and the diversion of reducing power to the formation of benzyl alcohol. in vivo enzymic activities were estimated from patterns of substrate u ...19751206374
[effect on growing conditions on protein content and amino acid composition of acinetobacter calcoaticus].the protein content and amino acid composition of acinetobacter calcoaceticus were studied as dependent on the stages of development and different hydrocarbon sources. the protein content was always the greatest at the logarithmic stage and decreased approaching the stationary stage. when cultivating on different hyrocarbon sources only an insignificant differedce in the content of protein was observed. a relative amino acid composition of ac. calcoaceticus remained practically constant during t ...19751209770
inhibition of the malic enzyme from acinetobacter calcoaceticus by nadph and nadh.the malic enzyme enriched from acinetobacter calcoaceticus is inhibited by nadph and nadh. the inhibition afforded by the reduced coenzymes is not affected by nad+, amp and 3'.5'-amp. against l-malate, nadph inhibits the enzyme in a noncompetitive linear fashion (ki = 1.5 x 10(-4) m), against nadp+, competitively linearly (ki = 5.0 x 10(-5) m). while nadph acted as a product inhibitor, nadh seems to be an allosteric effector of the malic enzyme, because with l-malate as the variable substrate in ...19759762
properties of the nadp+-dependent isocitrate dehydrogenases of acinetobacter calcoaceticus. 1975849
pyridine nucleotide-independent oxidation of long chain aliphatic alcohols by an enzyme of acinetobacter calcoaceticus. 1975850
fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria.the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ...197546774
the behaviour of nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation.the behaviour of the nicotinamide adenine dinucleotides nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation was studied, acetate and succinate being used as reference carbon sources. the intracellular concentration of the two nucleotides was found to increase during the exponential growth phase, reaching its maximum in the phase of decreasing growth rates. in the exponential phase, the nad+/nadh quotients were less than 1 and showed only unimportant variations. in the phase ...1975170172
the influence of changes in the environment on twitching examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. incubation should take place in a humid atmosphere. in fact, everything points to the humidity as a factor of the utmost importance. using strains of acinetobacter calcoaceticus, it ...1975239520
relationships among enzymes of the beta-ketoadipate pathway. iv. muconolactone isomerase from acinetobacter calcoaceticus and pseudomonas putida. 19744215816
[regulation of citrate synthase activity in acinetobacter calcoaceticus]. 19744371720
the effect of imidoesters on the protocatechuate 3,4-dioxygenase activity of acinetobacter calcoaceticus. 19744419544
dna polymerase i from acinetobacter calcoaceticus: enzymatic properties. 19744452356
multiple dna polymerases in acinetobacter calcoaceticus. 19744604798
effect of growth rate and nutrient limitation on the composition and biomass yield of acinetobacter calcoaceticus.acinetobacter calcoaceticus was grown on ethanol in a chemostat as a model system for single-cell protein production. the substrate yield coefficient (y(s), grams of biomass/gram of ethanol), protein yield coefficient (y(p), grams of protein/gram of ethanol), and biomass composition were measured as a function of the specific growth rate. nucleic acid, protein, y(p), and y(s) all increased at higher growth rates. although protein content increased only 14% (from 53 to 67%), y(p) almost doubled o ...19744844268
the nature of phospholipase c from acinetobacter calcoaceticus: effects on whole red cells and red cell membranes. 19734271908
anthranilate synthetase of acinetobacter calcoaceticus. separation and partial characterization of subunits. 19734349869
pyrimidine metabolism in acinetobacter calcoaceticus.the metabolism of thymine, thymidine, uracil, and uridine has been investigated in five different strains of acinetobacter calcoaceticus. attempts to isolate thymine and thymidine auxotrophic mutants were not successful. consistent with this finding was the observation that uptake of radioactive thymine or thymidine could not be demonstrated. search for enzymes capable of transforming thymine via thymidine to thymidine-5'-monophosphate in crude extracts was performed, and the following enzymes w ...19734355484
growth of acinetobacter calcoaceticus on ethanol.a soil microorganism, identified as acinetobacter calcoaceticus, was cultivated on ethanol as a sole source of carbon. this organism grew with a maximum specific growth rate of 0.7/h. the ph optimum for growth was between 6.5 and 7.5, and the temperature optimum was between 32 and 35 c. ethanol metabolism by this organism was inducible by ethanol, and the presence of acetate led to the repression of ethanol dehydrogenase. at higher cell densities the cessation of growth on ethanol was accompanie ...19734715559
role of carbohydrates in the metabolism of acinetobacter calcoaceticus. 19734748364
purification and properties of l-asparaginase a from acinetobacter calcoaceticus. 19734770738
leukolytic activity of acinetobacter calcoaceticus. 19724630253
properties of purified phospholipase c from acinetobacter calcoaceticus. 19724630254
acute villous inflammation in the placenta following intrauterine transfusion.infection is well recognized as a complication of intrauterine transfusion. the majority of cases are fortunately mild and consist merely of chorio-amnionitis. the present case, of severe type, resulted from contamination of the donor blood with acinetobacter calcoaceticus. spread of infection from foetus to mother has been carefully studied and an entirely new type of lesion in the placenta described. this takes the form of acute villous inflammation with resultant micro-abscess formation benea ...19724646299
mapping of the tryptophan genes of acinetobacter calcoaceticus by transformation.auxotrophs of acinetobacter calcoaceticus blocked in each reaction of the synthetic pathway from chorismic acid to tryptophan were obtained after n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis. one novel class was found to be blocked in both anthranilate and p-aminobenzoate synthesis; these mutants (trpg) require p-aminobenzoate or folate as well as tryptophan (or anthranilate) for growth. the loci of six other auxotrophic classes requiring only tryptophan were defined by growth, accumulation, ...19725086660
regulation of the synthesis of enzymes of tryptophan dissimilation in acinetobacter calcoaceticus. 19724117534
haemolytic activity of acinetobacter calcoaceticus. 19715280419
phospholipase activity of acinetobacter calcoaceticus. 19715283982
production of phospholipase c in acinetobacter calcoaceticus. 19715290406
comparison of the two isofunctional enol-lactone hydrolases from acinetobacter calcoaceticus.the rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (elh i and elh ii) of acinetobacter calcoaceticus were determined. the molecular weights of elh i and elh ii were found by gel filtration to be approximately 24,000 and 21,000, respectively. in crude extract at 45 c the two enzymes showed a marked difference in rate of thermal denaturation. after chromatography on sephadex g-100, however, the rates were nearly identical. the thermolabilit ...19715573730
regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 2. the role of protocatechuate as inducer. 19685645525
regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 4. constitutive synthesis of beta-ketoadipate succinyl-coa transferases ii and 3. 19685645526
regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 3. effects of 3-hydroxy-4-methylbenzoate on the synthesis of enzymes of the protocatechuate branch. 19685650850
regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 1. general aspects. 19676060184
[pathogenic or saprophytic bacteria transforming glucose to gluconic acid, bacterium anitratum, b5w, moraxella lwoffi var. glucidolytica, neisseria winogradskyi]. 195413171653
[degradation metabolism of 2-3 butanediol and of acetoin by microorganisms; considerations on neisseria winogradskyi. i. investigations on 2-3 butanediol dehydrogenase]. 195313124973
[utilization in competition of sodium beta-hydroxybutyrate by neisseria winogradskyi]. 195213017136
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