PMID(sorted ascending)
intensive care unit-acquired infections in a tertiary care hospital: an epidemiologic survey and influence on patient outcomes.nosocomial infections are common in intensive care units (icus), but the pattern of infections and the distribution of microorganisms vary. we studied the icu-acquired infections and their effect on patient outcomes in our icu.201626944004
acinetobacter seifertii isolated from china: genomic sequence and molecular epidemiology analyses.clinical infections caused by acinetobacter spp. have increasing public health concerns because of their global occurrence and ability to acquire multidrug resistance. acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex encompasses a. calcoaceticus, a. baumannii, a. pittii (formerly genomic species 3), and a nosocomial (formerly genomic species 13tu), which are predominantly responsible for clinical pathogenesis in the acinetobacter genus. in our previous study, a putative novel sp ...201626945401
blood stream infections caused by acinetobacter baumannii group in japan - epidemiological and clinical investigation.acinetobacter calcoaceticus-acinetobacter baumannii complex, especially a. baumannii, acinetobacter pittii and acinetobacter nosocomialis, constitutes an important group of nosocomial pathogens; however, epidemiological or clinical characteristics and prognosis is limited in japan. from 2009 to 2013, 47 blood stream infection cases resulting from a. baumannii group were reviewed at the national defense medical college, an 800-bed tertiary hospital. to determine the genospecies, further comparati ...201626993173
biodegradation of phenol by bacteria strain acinetobacter calcoaceticus pa isolated from phenolic wastewater.a phenol-degrading bacterium strain pa was successfully isolated from the effluent of petrochemical wastewater. based on its morphological, physiological and biochemical characteristics, the strain pa was characterized as a gram-negative, strictly aerobic, nonmotile and short rod-shaped bacterium that utilizes phenol as a sole carbon and energy source. 16s rdna sequence analysis revealed that this strain is affiliated to acinetobacter calcoaceticus in the group of gammaproteobacteria. the strain ...201627005648
antibiotic resistance profiles and genetic similarities within a new generation of carbapenem-resistant acinetobacter calcoaceticus-a. baumannii complex resistotypes in bosnia and herzegovina.acinetobacter calcoaceticus-a. baumannii complex (acb complex) is a nosocomial pathogen. due to its high ability to develop antibiotic resistance, it has become a problematic challenge in the modern healthcare system. the molecular and genetic mechanisms of gaining multidrug resistance in acb complex are well known. this study focuses on providing an overview of the antibiotic resistance profiles, genetic similarities and resistotypes, and general characteristics of carbapenem-resistant acb comp ...201627082787
isolation and characterization of fipronil degrading acinetobacter calcoaceticus and acinetobacter oleivorans from rhizospheric zone of zea enrichment culture technique was used for the isolation of bacteria capable of utilizing fipronil as a sole source of carbon and energy. based on morphological, biochemical characteristics and phylogenetic analysis of 16s rrna sequence, the bacterial strains were identified as acinetobacter calcoaceticus and acinetobacter oleivorans. biodegradation experiments were conducted in loamy sand soil samples fortified with fipronil (50 µg kg(-1)) and inoculated with acinetobacter sp. cells (45 × 10( ...201627084098
the effects of sodium hypochlorite against selected drinking water-isolated bacteria in planktonic and sessile states. 201627156214
molecular epidemiology and clinical impact of acinetobacter calcoaceticus-baumannii complex in a belgian burn wound center.multidrug resistant acinetobacter baumannii and its closely related species a. pittii and a. nosocomialis, all members of the acinetobacter calcoaceticus-baumannii (acb) complex, are a major cause of hospital acquired infection. in the burn wound center of the queen astrid military hospital in brussels, 48 patients were colonized or infected with acb complex over a 52-month period. we report the molecular epidemiology of these organisms, their clinical impact and infection control measures taken ...201627223476
effect of plant growth-promoting bacteria on the growth and fructan production of agave americana l.the effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in agave americana was assessed. the bacterial strains aco-34a, aco-40, and aco-140, isolated from the a. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. the three bacterial strains were evaluated via plant inoculation assays, and azospirillum brasilense cd served as a control strain. phylogenetic analysis based on the 16s rrna gene showe ...201627268113
development and evaluation of species-specific pcr for detection of nine acinetobacter species.molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ...201627312551
incidence, management, and visual outcomes in pediatric endophthalmitis following cataract surgery by a single report the incidence of endophthalmitis following cataract surgery by a single surgeon in children and to describe its microbiological profile, management, and visual outcomes.201627343836
structural basis of selective aromatic pollutant sensing by the effector binding domain of mopr, an ntrc family transcriptional regulator.phenol and its derivatives are common pollutants that are present in industrial discharge and are major xenobiotics that lead to water pollution. to monitor as well as improve water quality, attempts have been made in the past to engineer bacterial in vivo biosensors. however, due to the paucity of structural information, there is insufficiency in gauging the factors that lead to high sensitivity and selectivity, thereby impeding development. here, we present the crystal structure of the sensor ...201627362503
rapid species identification and epidemiological analysis of carbapenem-resistant acinetobacter spp. by a pcr-based open reading frame typing method.the spread of carbapenem-resistant acinetobacter spp. has become a global problem. in this study, 18 carbapenem-resistant acinetobacter calcoaceticus-baumannii (acb) complexes, identified using a conventional biochemical method at our hospital during 2004-2013, were studied for species identification and epidemiological analyses. species identification was performed using matrix-assisted laser desorption ionization-time-of-flight ms, a partial sequence analysis of rpob and a pcr-based orf typing ...201627412356
acinetobacter dijkshoorniae sp. nov., a member of the acinetobacter calcoaceticus-acinetobacter baumannii complex mainly recovered from clinical samples in different countries.the recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of the genus acinetobacter. in the present study, phenotypic and molecular methods have been used to determine the taxonomic position of a group of 12 genotypically distinct strains belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, initially described by gerner-smidt and tjernberg in 1993, that are closely related to acinetobacter pittii. strains chara ...201627432448
characteristics of invasive acinetobacter species isolates recovered in a pediatric academic center.acinetobacter species are associated with increasing mortality due to emerging drug-resistance. pediatric acinetobacter infections are largely undefined in developed countries and clinical laboratory identification methods do not reliably differentiate between members of the acinetobacter calcoaceticus-baumannii complex, leading to improper identification. therefore we aimed to determine risk factors for invasive acinetobacter infections within an academic, pediatric setting as well as defining ...201627449800
tigecycline-based versus sulbactam-based treatment for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex.the treatment options for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii (mdr acb) complex are limited, and the optimal treatment has not been established.201627496018
the role of the genetic elements bla oxa and is aba 1 in the acinetobacter calcoaceticus-acinetobacter baumannii complex in carbapenem resistance in the hospital setting.members of the acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. oxacillinases are primarily responsible for resistance to carbapenem antibiotics. higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the isaba1 sequence, near bla oxa genes. the present study examined the occurrence of the genetic elements bla oxa and isaba1 and their relationship with susc ...201727598629
pcr assay based on the gyrb gene for rapid identification of acinetobacter baumannii-calcoaceticus complex at specie level.the genus acinetobacter sp. comprises more than 50 species, and four are closely related and difficult to be distinguished by either phenotypic or genotypic methods: the acinetobacter calcoaceticus-baumannii complex (abc). the correct identification at species level is necessary mainly due to the epidemiological aspects.201627605498
opportunistic infections in hiv-infected patients differ strongly in frequencies and spectra between patients with low cd4+ cell counts examined postmortem and compensated patients examined antemortem irrespective of the haart era.aids-related mortality has changed dramatically with the onset of highly active antiretroviral therapy (haart), which has even allowed compensated hiv-infected patients to withdraw from secondary therapy directed against opportunistic pathogens. however, in recently autopsied hiv-infected patients, we observed that associations with a broad spectrum of pathogens remain, although detailed analyses are lacking. therefore, we focused on the possible frequency and spectrum shifts in pathogens associ ...201627611681
multi-drug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex infection outbreak in dogs and cats in a veterinary hospital.members of the acinetobacter calcoaceticus-acinetobacter baumannii complex cause severe outbreaks in humans, and are increasingly reported in animals.201627709647
emergence of new delhi metallo-beta-lactamase 1 and other carbapenemase-producing acinetobacter calcoaceticus-baumannii complex among patients in hospitals in ha noi, viet nam.acinetobacter baumannii is an important cause of multidrug-resistant hospital acquired infections in the world. here, we investigate the presence of ndm-1 and other carbapenemases among carbapenem-resistant a. baumannii isolated between august 2010 and december 2014 from three large hospitals in hanoi, vietnam. we identified 23/582 isolates (4 %) (11 from hospital a, five from hospital b, and seven from hospital c) that were ndm-1 positive, and among them 18 carried additional carbapenemase gene ...201727714593
evaluation of an ultraviolet c light-emitting device for disinfection of electronic devices.a tabletop-type ultraviolet c (uvc) light-emitting disinfecting device was evaluated for microbiologic effectiveness, safety, usability, and end-user satisfaction.201627742144
engineering cyclohexanone monooxygenase for the production of methyl propanoate.a previous study showed that cyclohexanone monooxygenase from acinetobacter calcoaceticus (acchmo) catalyzes the baeyer-villiger oxidation of 2-butanone, yielding ethyl acetate and methyl propanoate as products. methyl propanoate is of industrial interest as a precursor of acrylic plastic. here, various residues near the substrate and nadp(+) binding sites in acchmo were subjected to saturation mutagenesis to enhance both the activity on 2-butanone and the regioselectivity toward methyl propanoa ...201727935281
clinical and pathophysiological overview of acinetobacter infections: a century of challenges.summaryacinetobacter is a complex genus, and historically, there has been confusion about the existence of multiple species. the species commonly cause nosocomial infections, predominantly aspiration pneumonia and catheter-associated bacteremia, but can also cause soft tissue and urinary tract infections. community-acquired infections by acinetobacter spp. are increasingly reported. transmission of acinetobacter and subsequent disease is facilitated by the organism's environmental tenacity, resi ...201727974412
draft genome sequence of jvap01t, the type strain of the novel species acinetobacter, we report the draft genome sequence of the type strain of acinetobacter dijkshoorniae, a novel human pathogen within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. strain jvap01(t) has an estimated genome size of 3.9 mb, exhibits a 38.8% g+c content, and carries a plasmid with the blandm-1 carbapenemase gene.201728082506
clinical and microbiological characteristics of bloodstream infections among patients with haematological malignancies with and without neutropenia at a medical centre in northern taiwan, 2008-2013.this study aimed to investigate the epidemiology and microbiology of bloodstream infections (bsis) in patients with haematological malignancies. clinical characteristics and microbiology of 2083 patients with haematological malignancy who were treated for bsi from 2008 to 2013 at a medical centre in taiwan were retrospectively reviewed. lymphoma (38.1%) was the most common, followed by acute myeloid leukaemia (30.9%). of the 2090 non-duplicate bsi isolates, 1310 (62.7%) were recovered from patie ...201728109554
effects of meropenem exposure in persister cells of acinetobacter evaluate the influence of meropenem in the acinetobacter calcoaceticus-baumannii (acb) persister levels.201728111984
lettuce and fruits as a source of multidrug resistant acinetobacter spp.the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ...201728213015
novel oxadiazole thioglycosides as potential anti-acinetobacter agents.the glycosylation of 1,3,4-oxadiazole-2-thiones has been performed with peracetylated β-pyranosyl bromide in the presence of potassium carbonate. deprotection of acetylated thioglycosides was necessary for increasing their antibacterial effects. the structures of nucleosides were confirmed by (1)h nmr, (13)c nmr and hrms. the anomeric protons of nucleosides c1-4 were assigned to the doublet, confirming the β-configuration. the synthesized compounds were tested for their antimicrobial activity ag ...201628243273
complete genome sequence and methylome analysis of acinetobacter calcoaceticus 65.acinetobacter calcoaceticus 65 is the original source strain for the restriction enzyme acc65i. its complete sequence and full methylome were determined using single-molecule real-time (smrt) sequencing.201728336599
epidemiology and trends in the antibiotic susceptibilities of gram-negative bacilli isolated from patients with intra-abdominal infections in the asia-pacific region, 2010-2013.this study was conducted to investigate the epidemiology and antimicrobial susceptibility patterns of gram-negative bacilli (gnb) isolated from intra-abdominal infections (iais) in the asia-pacific region (apr) from 2010-2013. a total of 17 350 isolates were collected from 54 centres in 13 countries in the apr. the three most commonly isolated gnb were escherichia coli (46.1%), klebsiella pneumoniae (19.3%) and pseudomonas aeruginosa (9.8%). overall, the rates of extended-spectrum β-lactamase (e ...201728435019
carbapenem-resistant acinetobacter baumannii contamination in an intensive care unit.acinetobacter baumannii is a major pathogen causing infections in intensive care units (icus). in this study, we aimed to evaluate the presence of a. baumannii in an icu environment and gloves from icu workers and to characterize the antimicrobial resistance of the isolates in comparison with those isolated from icu patients at the same hospital.201728562751
development and evaluation of a core genome multilocus typing scheme for whole-genome sequence-based typing of acinetobacter baumannii.we have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgmlst) scheme for acinetobacter baumannii. to define a core genome we downloaded a total of 1,573 putative a. baumannii genomes from ncbi as well as representative isolates belonging to the eight previously described international a. baumannii clonal lineages. the core genome was then employed against a total of fifty-three carbapenem-resistant a. baumannii isolates that were previously typ ...201728594944
characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation.bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. in this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for diesel-degrading potential, biosurfactant production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. diesel degradation rates were determined in vitro in minimal med ...201728598213
complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin.we report here the complete assembled genome sequence of acinetobacter calcoaceticus ca16, which is capable of utilizing diesel and lignin as a sole carbon source. ca16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. the assembled sequences will help elucidate potential metabolic pathways and mechanisms responsible for ca16's hydrocarbon degradation ability.201728619790
first report of cavitary pneumonia due to community-acquired acinetobacter pittii, study of virulence and overview of pathogenesis and treatment.acinetobacter pittii is a nosocomial pathogen rarely involved in community-acquired infections. we report for the first time that a. pittii can be responsible for cavitary community-acquired pneumonia and study its virulence, and discuss its pathogenesis and treatment options.201728683724
structure guided design of protein biosensors for phenolic pollutants.phenolic aromatic compounds are a major source of environmental pollution. currently there are no in situ methods for specifically and selectively detecting these pollutants. here, we exploit the nature's biosensory machinery by employing acinetobacter calcoaceticus ncib8250 protein, mopr, as a model system to develop biosensors for selective detection of a spectrum of these pollutants. the x-ray structure of the sensor domain of mopr was used as a scaffold for logic-based tunable biosensor desi ...201728723202
erratum for ho et al., "complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin". 201728774994
an in vitro synthetic biosystem based on acetate for production of phloroglucinol.phloroglucinol is an important chemical, and the biosynthesis processes which can convert glucose to phloroglucinol have been established. however, due to approximate 80% of the glucose being transformed into undesirable by-products and biomass, this biosynthesis process only shows a low yield with the highest value of about 0.20 g/g. the industrial applications are usually hindered by the low current productivity and yield and also by the high costs. generally, several different aspects limit t ...201728789688
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