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biodegradation of triton x-100 and its primary metabolites by a bacterial community isolated from activated sludge.a set of studies was carried using a continuous flow biodegradation unit in order to isolate a microbial community capable of efficient and complete utilization of octylphenol ethoxylates from activated sludge. increasing concentrations of triton x-100 (in the range of 1-1000 mg/l) were applied over a time period of 35 days in order to select microorganisms, which exhibit high tolerance towards this surfactant. the fate of the surfactant and its primary degradation products was assessed by hplc/ ...201323770380
isolation and structural characterization of a (kdo-isosteric) d-glycero-α-d-talo-oct-2-ulopyranosidonic acid (ko) interlinking lipid a and core oligosaccharide in the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305.in acinetobacter calcoaceticus nctc 10305 and a. haemolyticus nctc 10305 lipopolysaccharide (lps) a kdo (3-deoxy-d-manno-oct-2-ulosonic acid)-related octulosonic acid (ko) interlinks the lipid a with the core-oligosaccharide. this ko replaces the first kdo (kdo(i)) attached to the lipid a backbone in the lps. the only structural difference between kdo and ko is the 3-hydroxylation. after the discovery of the final step in ko-biosynthesis it is now generally accepted that ko is structurally relat ...201323743267
development of a whole-cell biocatalyst with nadph regeneration system for biosulfoxidation.a formate dehydrogenase gene (fdh) originated from candida boidinii was co-expressed in e. coli bl21 (de3) with the cyclohexanone monooxygenase gene (chmo) cloned from acinetobacter calcoaceticus ncimb 9871. the co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide (pmso) from thioanisole (pms) and the reaction conditions were investigated. when the initial concentration of pms was 20 mm, the specific productivity of pmso in this system was 2 ...201323729190
[effect of univalent cations on synthesis of surfactants by acinetobacter calcoaceticus imv b-7241].the effect of univalent cations on activity of key enzymes of c2-metabolism has been investigated in the producer of biosurfactants, acinetibacter calcoaceticus imv b-7241 grown on ethanol. it was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (nadp-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (phep)-carboxikinase), while ammonium cati ...201323720959
identification and functional characteristics of chlorpyrifos-degrading and plant growth promoting bacterium acinetobacter calcoaceticus.a bacterial strain d10 with strong ability of degrading chlorpyrifos was isolated from rhizosphere of chives contaminated with pesticide. it was found that it's capable of utilizing chlorpyrifos as the sole source of carbon for growth, and within the first 4 days the extent of degradation at initial concentration of 100 mg l(-1) was 60.0%. it also showed a high ability of degrading chlorpyrifos in sterilized soil, and the degradation reached up to 60.2% after 18 days. in addition, the strain d10 ...201423712768
acinetobacter pittii and acinetobacter nosocomialis among clinical isolates of the acinetobacter calcoaceticus-baumannii complex in sichuan, china.among 82 clinical isolates of the acinetobacter calcoaceticus-baumannii complex recovered in 13 hospitals of sichuan, china, in 2011, 13 were acinetobacter pittii and 2 were acinetobacter nosocomialis. multilocus sequence typing revealed a novel sequence type (st) of a. nosocomialis and 7 novel sts of a. pittii. most isolates were hospital-acquired and colonized in the respiratory tract, while 6 cases with pneumonia due to a. pittii were identified. this study provided a snapshot of the local in ...201323639796
label-free and selective bacteria detection using a film with transferred bacterial configuration.specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface of overoxidized polypyrrole (oppy) film. the recognition of target bacteria was successfully carried out in real time using oppy film in combination with dielectrophoresis. the unique combination of both techniques made the specific detection of template bacilli possible at concentrations as low as 10(3) cfu/ml within 3 min, without any bacterial pretreatment. the observation of ...201323577783
inoculation of phosphate solubilizing bacteria for the improvement of lead accumulation by brassica juncea.two phosphate-solubilizing bacterial strains were isolated and identified as acinetobacter calcoaceticus yc-5a and enterobacter agglomerans kmc-7 based on the 16s rrna gene sequence analysis. a. calcoaceticus yc-5a is less well known as a phosphate-solubilizing plant-associated bacterium. the plant growth-promoting properties of the phosphate-solubilizing bacteria (psb) were characterized in vitro, including their phosphate-solubilizing activities and their capabilities for producing indole-3-ac ...201323530360
[effect of cu2+ on synthesis of biosurfactants of acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5017].synthesis of biosurfactants (surface-active substances, sas) was investigated under the conditions of growth of rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241 on hydrophobic (n-hexadecane, liquid paraffins, sunflower oil) and hydrophilic (ethanol) substrates depending on concentration (0.01-0.5 mm) and time of copper cations introduction in the medium. it is established that cu2+ addition in the exponential phase of growth of the strains imv b-7241 and imv ac-501 ...201623516834
emerging threat of multidrug resistant bugs--acinetobacter calcoaceticus baumannii complex and methicillin resistant staphylococcus aureus.infections caused by bacteria such as multidrug resistant (mdr) acinetobacter spp. and methicillin-resistant staphylococcus aureus (mrsa) constitute a worldwide pandemic. without gathering information about these strains, we cannot reduce the morbidity and mortality due to infections caused by these notorious bugs.201323497675
repeated colonization by multi-drug-resistant acinetobacter calcoaceticus-a. baumannii complex and changes in antimicrobial susceptibilities in surgical intensive care units.a nosocomial outbreak of multi-drug-resistant acinetobacter calcoaceticus-a. baumannii (mdr-acb) complex infection occurred in a newly constructed building at a 2,500-bed tertiary medical center in taiwan.201323425282
appraising freeze-drying for storage of bacteria and their ready access in a rapid toxicity assessment assay.direct toxicity assessment (dta) techniques seek to measure the impact of toxic chemicals on biological materials resident in the environment. this study features the use of freeze-dried bacterial cells in combination with a rapid dta analyser, scitox. the effects of three factors-cryoprotectant type, bacterial strain, and storage temperature-were tested in order to validate the shelf life of the freeze-dried cells. three freeze-dried gram-negative bacterial strains, acinetobacter calcoaceticus, ...201323377787
[production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol with organic acids].the effect of fumarate (c4-dicarboxylic acid, a gluconeogenesis precursor) and citrate (a lipid synthesis regulator) on the production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol has been studied. simultaneous addition of fumarate and citrate to concentrations of 0.01-0.02% at the end of the log phase of k-4 growth in a medium with 2 vol% ethanol increases the nominal surfactant concentration by 45-55% in comparison with a culture without organic acids. the increased level ...201323330390
antibacterial and antiplasmodial constituents of beilschmiedia cryptocaryoides.four new beilschmiedic acid derivatives, cryptobeilic acids a-d (1-4), and tsangibeilin b (5) have been isolated from the bark of beilschmiedia cryptocaryoides collected from madagascar. their structures were elucidated using detailed spectroscopic and spectrometric methods. cryptobeilic acid a (1) and tsangibeilin b (5) structures were confirmed by single-crystal x-ray diffraction analysis. compounds 1-5 displayed moderate antibacterial activity against escherichia coli 6r3, acinetobacter calco ...201323320609
[effects of oil-refining microbes (genus acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].the objective of this study was to assess ability of oil-refining bacteria acinetobacter calcoaceticus and a. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. it was found that the cultures infected with a. calcoaceticus showed significantly high frequencies of cytogenetical effects and chromosomal aberrant cells as compared to the intact cultures and cultures infected with a. valentis. the most of chromosomal aberrations, mainly chroma ...201323285752
[intensification of biosurfactant synthesis by acinetobacter calcoaceticus imv b-7241 on a hexadecane–glycerol mixture ]. 201323234072
cloning and characterization of the l-ribose isomerase gene from cellulomonas parahominis mb426.a newly isolated bacterium, cellulomonas parahominis mb426, produced l-ribose isomerase (celri) on a medium containing l-ribose as a sole carbon source. a 32 kda protein isomerizing l-ribose to l-ribulose was purified to homogeneity from this bacterium. a set of degenerated primers were synthesized based on amino acid sequences of the purified celri, and a 747 bp gene encoding celri was cloned, sequenced and overexpressed in escherichia coli. this gene encoded a 249 amino acid protein with a cal ...201323207370
biodegradation of the herbicide propanil, and its 3,4-dichloroaniline by-product in a continuously operated biofilm reactor.the persistence of propanil in soil and aquatic environments along with the possible accumulation of toxic degradation products, such as chloroanilines, is of environmental concern. in this work, a continuous small-scale bioprocess to degrade the herbicide propanil, its main catabolic by-product, 3,4-dichloroaniline (3,4-dca), and the herbicide adjuvants is carried out. a microbial consortium, constituted by nine bacterial genera, was selected. the isolated strains, identified by amplification a ...201323117676
antibiotic resistance patterns of acinetobacter calcoaceticus-a. baumannii complex species from colombian hospitals.only automated phenotypic methods are currently used in colombian hospitals for identifying isolates of the acinetobacter calcoaceticus-a. baumannii complex (acb). the phenotypical similarities in these species mean that they cannot be differentiated by manual or automated methods, thereby leading to their identification as a. baumannii, or acb complex in clinical settings. our objective was to identify to the species level 60 isolates, from four hospitals, evaluate their antibiotic susceptibili ...201323021300
multitrophic interaction in the rhizosphere of maize: root feeding of western corn rootworm larvae alters the microbial community composition.larvae of the western corn rootworm (wcr) feeding on maize roots cause heavy economical losses in the us and in europe. new or adapted pest management strategies urgently require a better understanding of the multitrophic interaction in the rhizosphere. this study aimed to investigate the effect of wcr root feeding on the microbial communities colonizing the maize rhizosphere.201222629377
a five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by acinetobacter radioresistens sh164.acinetobacter calcoaceticus sw1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (tauxy). blast searches of newly available genome sequences using the tauxy sequences revealed a 5-gene cluster, taurxypi, in acinetobacter radioresistens sh164. we hypothesized that tauxypi (hmpref0018_00717-hmpref0018_00720) encodes proteins that are orthologs of the undefined pathway from st ...201222588221
[synthesis of surfactants acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5070 in the medium with glycerol].it was established that glycerol, a byproduct of biodiesel production, may be used as substrate for synthesis of surfactants rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241. maximum indices of surfactants synthesis by the strain imv b-7241 have been fixed, when the medium with glycerol included yeast autolysate and trace elements. it was shown that the surfactants synthesis could be intensified when cultivating a. calcoaceticus imv b-7241 and r. erythropolis imv a ...201322545440
domiciliary cockroaches found in restaurants in five zones of kuala lumpur federal territory, peninsular malaysia.the following domiciliary cockroaches were collected from restaurants in five zones of kuala lumpur federal territory, malaysia using 1l glass beaker traps baited with ground mouse-pellets: periplaneta americana (linnaeus) (n = 820), periplaneta brunnea burmeister (n = 46), blattella germanica (linnaeus) (n = 12504), supella longipalpa (fabricius) (n = 321), symploce pallens stephens (n = 29) and neostylopyga rhombifolia (stoll) (n = 5). the following bacteria were isolated from 10 cockroach spe ...201222543619
infections due to different genospecies of the acinetobacter calcoaceticus-a baumannii complex. 201222526214
vertical immobilization of viable bacilliform bacteria into polypyrrole films.we have successfully developed a novel technique for inserting viable bacilliform bacteria into polypyrrole films. all of the five different bacterial cells (pseudomonas aeruginosa, acinetobacter calcoaceticus, serratia marcescens, bacillus subtilis and escherichia coli) studied in this work were inserted normal to the film surface, and the viability of p. aeruginosa was unaffected by this immobilization procedure. it was also found that the polypyrrole layer was important to keep the cells aliv ...201222498456
polypeptide requirement of multicomponent monooxygenase dsoabcdef for dimethyl sulfide oxidizing activity.in our previous study, the multicomponent monooxygenase dsoabcdef in acinetobacter sp. strain 20b was cloned based on its ability to oxidize dimethyl sulfide (dms) to dimethyl sulfoxide (dmso) in e. coli cells, which had high sequence similarity with phenol hydroxylase mopklmnop in acinetobacter calcoaceticus ncib8250, dmpklmnop in pseudomonas sp. cf600 and some other multicomponent monooxygenases. in this study, dsob, c, d, e, and f were found to be needed for dms-oxidizing activity in polypept ...199926300166
2,2'-oxo-1,1'-azobenzene: microbial transformation of rye (secale cereale l.) allelochemical in field soils byacinetobacter calcoaceticus: iii.acinetobacter calcoaceticus, a gram-negative bacterium isolated from field soil, was found to be responsible for the biotransformation of 2(3h)-benzoxazolinone (boa) to 2,2'-oxo-1,1'-azobenzene (azob). experiments were conducted to evaluate the transformation of boa to azob by this microbe in sterile and nonsterile soil. transformation studies with soils inoculated witha. calcoaceticus indicated that the production of azob increased linearly with the concentration of boa in sterile soil and show ...199124257881
bacterial desorption from food container and food processing surfaces.the desorption ofstaphylococcus aureus, acinetobacter calcoaceticus, and a coryneform from the surfaces of materials used for manufacturing food containers (glass, tin plate, and polypropylene) or postprocess canning factory conveyor belts (stainless steel and nylon) was investigated. the effect of time, ph, temperature, and adsorbed organic layers on desorption was studied.s. aureus did not detach from the substrata at any ph investigated (between ph 5 and 9).a. calcoaceticus and the coryneform ...198824203003
serological studies on chloridazon-degrading bacteria.agglutination tests and immunofluorescence tests with antisera against four strains of chloridazon-degrading bacteria revealed the serological uniformity of a group of 22 chloridazon-degrading bacterial strains. no serological relationship could be found between chloridazon-degrading bacteria and representatives of other gram-negative bacteria. this was demonstrated by agglutination tests, including testing of the antiserum against acinetobacter calcoaceticus, and by immunofluorescence tests, in ...198323194804
degradation of aromatic carboxylic acids by acinetobacter.the degradation of the aromatic carboxylic acids 3-phenylpropionic acid, cinnamic acid and l-phenylalanine was investigated in several strains of acinetobacter calcoaceticus var. lwoffii. evidence is presented for the conversion of 3-phenylpropionic acid into the cis-2,3-dihydrodihydroxy-derivative, which is further metabolized to 3-(2,3-dihydroxyphenyl-)propionic acid, followed by cleavage of the aromatic ring in meta-proximal position. when pre-grown on 3-phenylpropionic acid, cinnamic acid as ...198323194732
an in vitro synthetic biosystem based on acetate for production of phloroglucinol.phloroglucinol is an important chemical, and the biosynthesis processes which can convert glucose to phloroglucinol have been established. however, due to approximate 80% of the glucose being transformed into undesirable by-products and biomass, this biosynthesis process only shows a low yield with the highest value of about 0.20 g/g. the industrial applications are usually hindered by the low current productivity and yield and also by the high costs. generally, several different aspects limit t ...201728789688
development and evaluation of a core genome multilocus typing scheme for whole-genome sequence-based typing of acinetobacter baumannii.we have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgmlst) scheme for acinetobacter baumannii. to define a core genome we downloaded a total of 1,573 putative a. baumannii genomes from ncbi as well as representative isolates belonging to the eight previously described international a. baumannii clonal lineages. the core genome was then employed against a total of fifty-three carbapenem-resistant a. baumannii isolates that were previously typ ...201728594944
carbapenem-resistant acinetobacter baumannii contamination in an intensive care unit.acinetobacter baumannii is a major pathogen causing infections in intensive care units (icus). in this study, we aimed to evaluate the presence of a. baumannii in an icu environment and gloves from icu workers and to characterize the antimicrobial resistance of the isolates in comparison with those isolated from icu patients at the same hospital.201728562751
erratum for ho et al., "complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin". 201728774994
structure guided design of protein biosensors for phenolic pollutants.phenolic aromatic compounds are a major source of environmental pollution. currently there are no in situ methods for specifically and selectively detecting these pollutants. here, we exploit the nature's biosensory machinery by employing acinetobacter calcoaceticus ncib8250 protein, mopr, as a model system to develop biosensors for selective detection of a spectrum of these pollutants. the x-ray structure of the sensor domain of mopr was used as a scaffold for logic-based tunable biosensor desi ...201728723202
first report of cavitary pneumonia due to community-acquired acinetobacter pittii, study of virulence and overview of pathogenesis and treatment.acinetobacter pittii is a nosocomial pathogen rarely involved in community-acquired infections. we report for the first time that a. pittii can be responsible for cavitary community-acquired pneumonia and study its virulence, and discuss its pathogenesis and treatment options.201728683724
complete genome sequence of acinetobacter calcoaceticus ca16, a bacterium capable of degrading diesel and lignin.we report here the complete assembled genome sequence of acinetobacter calcoaceticus ca16, which is capable of utilizing diesel and lignin as a sole carbon source. ca16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. the assembled sequences will help elucidate potential metabolic pathways and mechanisms responsible for ca16's hydrocarbon degradation ability.201728619790
characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation.bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. in this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for diesel-degrading potential, biosurfactant production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. diesel degradation rates were determined in vitro in minimal med ...201728598213
epidemiology and trends in the antibiotic susceptibilities of gram-negative bacilli isolated from patients with intra-abdominal infections in the asia-pacific region, 2010-2013.this study was conducted to investigate the epidemiology and antimicrobial susceptibility patterns of gram-negative bacilli (gnb) isolated from intra-abdominal infections (iais) in the asia-pacific region (apr) from 2010-2013. a total of 17 350 isolates were collected from 54 centres in 13 countries in the apr. the three most commonly isolated gnb were escherichia coli (46.1%), klebsiella pneumoniae (19.3%) and pseudomonas aeruginosa (9.8%). overall, the rates of extended-spectrum β-lactamase (e ...201728435019
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