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discrimination of the acinetobacter calcoaceticus-acinetobacter baumannii complex species by fourier transform infrared spectroscopy.the main goal of this work was to assess the ability of fourier transform infrared spectroscopy with attenuated total reflectance (ftir-atr) to discriminate between the species of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, i.e. a. baumannii, a. nosocomialis, a. pittii, a. calcoaceticus, genomic species "between 1 and 3" and genomic species "close to 13tu". a total of 122 clinical isolates of the acb complex previously identified by rpob sequencing were studied. ftir-a ...201424563251
identification of 50 class d β-lactamases and 65 acinetobacter-derived cephalosporinases in acinetobacter spp.whole-genome sequencing of a collection of 103 acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class d β-lactamases and 65 new acinetobacter-derived cephalosporinases (adc). all oxacillinases (oxa) contained the three typical motifs of class d β-lactamases, stfk, (f/y)gn, and k(s/t)g. the phylogenetic tree drawn from the oxa sequences led to an increase in the number of oxa groups from 7 to 18. the topologies of th ...201424277043
types and prevalence of carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex in northern taiwan.the frequency of the carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii (cracb) complex increases annually in our hospitals. however, the types and prevalence of carbapenemases among isolates still remain unclear. in this study, we identified and collected 672 carbapenem-resistant isolates from a medical center in northern taiwan between april and december of 2010. there were 577 genospecies 2 (acinetobacter baumannii), 79 genospecies 13tu, and 16 genospecies 3 isolates. th ...201424145535
[overexpression, purification and characterization of phospholipase c from acinetobacter calcoaceticus].in this study, we constructed two recombinant escherichia coli strains to produce phospholipase c (plc) from acinetobacter calcoaceticus. the recombinant enzymes were purified to homogeneity and characterized. [methods] we cloned the plc encoding gene plc1, plc2 from genome dna of a. calcoaceticus atcc17902. the amplified fragments were inserted into pet28a(+ to obtain expression plasmids. e. coli bl21 (de3) harboring the above plasmids were cultivated and induced with isopropyl-beta-d-thiogalac ...201425803900
multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp.bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ...201424288029
identification and functional characteristics of chlorpyrifos-degrading and plant growth promoting bacterium acinetobacter calcoaceticus.a bacterial strain d10 with strong ability of degrading chlorpyrifos was isolated from rhizosphere of chives contaminated with pesticide. it was found that it's capable of utilizing chlorpyrifos as the sole source of carbon for growth, and within the first 4 days the extent of degradation at initial concentration of 100 mg l(-1) was 60.0%. it also showed a high ability of degrading chlorpyrifos in sterilized soil, and the degradation reached up to 60.2% after 18 days. in addition, the strain d10 ...201423712768
characterization and fungal inhibition activity of siderophore from wheat rhizosphere associated acinetobacter calcoaceticus strain hirfa32.acinetobacter calcoaceticus hirfa32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without feso4 at 28 °c and 24 h of incubation. hplc purified siderophore appeared as pale yellow crystals with molecular weight [m(+1)] m/z 347.18 estimated by lcms. the structure elucidated by (1)h nmr, (13)c nmr, hmqc, hmbc, noesy and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid w ...201424891739
characterization of affinity-purified isoforms of acinetobacter calcoaceticus y1 glutathione transferases.glutathione transferases (gst) were purified from locally isolated bacteria, acinetobacter calcoaceticus y1, by glutathione-affinity chromatography and anion exchange, and their substrate specificities were investigated. sds-polyacrylamide gel electrophoresis revealed that the purified gst resolved into a single band with a molecular weight (mw) of 23 kda. 2-dimensional (2-d) gel electrophoresis showed the presence of two isoforms, gst1 (pi 4.5) and gst2 (pi 6.2) with identical mw. gst1 was reac ...201424892084
a new double digestion ligation mediated suppression pcr method for simultaneous bacteria dna-typing and confirmation of species: an acinetobacter sp. model.we have designed a new ddlms pcr (double digestion ligation mediated suppression pcr) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. the ddlms pcr combines a simple pcr used for species or genus identification and the lm pcr strategy for strain differentiation. the bacterial identification is confirmed in the form of the pcr product(s), while the length of the pcr product makes ...201425522278
epidemiological characteristics of blandm-1 in enterobacteriaceae and the acinetobacter calcoaceticus-acinetobacter baumannii complex in china from 2011 to 2012.the study aimed to investigate the prevalence and epidemiological characteristics of blandm-1 (encoding new delhi metallo-β-lactamase 1) in enterobacteriaceae and the acinetobacter calcoaceticus-acinetobacter baumannii complex (abc) in china from july 2011 to june 2012.201425469701
evaluation of clonality and carbapenem resistance mechanisms among acinetobacter baumannii-acinetobacter calcoaceticus complex and enterobacteriaceae isolates collected in european and mediterranean countries and detection of two novel β-lactamases, ges-22 and vim-35.we evaluated doripenem-resistant acinetobacter baumannii-acinetobacter calcoaceticus complex (acb; n = 411) and enterobacteriaceae (n = 92) isolates collected from patients from 14 european and mediterranean countries during 2009 to 2011 for the presence of carbapenemase-encoding genes and clonality. following susceptibility testing, carbapenem-resistant (doripenem mic, >2 μg/ml) isolates were screened for carbapenemases. new β-lactamase genes were expressed in a common background and susceptibi ...201425267671
class 1 integrons and antibiotic resistance of clinical acinetobacter calcoaceticus-baumannii complex in poznań, poland.sixty-three clinical isolates of acinetobacter calcoaceticus-baumannii complex were analyzed for the presence of integrons and antimicrobial resistance. class 1 integrons were detected in 40 (63.5 %) isolates. none of them had class 2 or class 3 integrons. the majority of the integrons contained aacc1-orfa-orfb-aada1 gene cassette array. the presence of integrons was associated with the increased frequency of resistance to 12 of 15 antimicrobials tested, multi-drug resistance phenotype, and the ...201424740302
active surveillance for asymptomatic colonization with multidrug-resistant gram negative bacilli among injured service members--a three year evaluation.in response to the high rates of colonization and infection by multidrug-resistant gram-negative bacilli (mdr gnb), many military treatment facilities (mtfs) have implemented additional infection control practices, such as active surveillance cultures for asymptomatic colonization. results of surveillance cultures (june 2009 - may 2012) collected from patients at landstuhl regional medical center (landstuhl rmc), germany, and three u.s. mtfs were analyzed to evaluate trends in mdr gnb colonizati ...201324011372
increased lysine content is the main characteristic of the soluble form of the polyamide cyanophycin synthesized by recombinant escherichia coli.cyanophycin, a polyamide of cyanobacterial or noncyanobacterial origin consisting of aspartate, arginine, and lysine, was synthesized in different recombinant strains of escherichia coli expressing cpha from synechocystis sp. strain pcc 6308 or pcc 6803, anabaena sp. strain pcc 7120, or acinetobacter calcoaceticus adp1. the molar aspartate/arginine/lysine ratio of the water-soluble form isolated from a recombinant strain expressing cpha6308 was 1:0.5:0.5, with a lysine content higher than any ev ...201323686266
evolution of a pathogen: a comparative genomics analysis identifies a genetic pathway to pathogenesis in acinetobacter.acinetobacter baumannii is an emergent and global nosocomial pathogen. in addition to a. baumannii, other acinetobacter species, especially those in the acinetobacter calcoaceticus-baumannii (acb) complex, have also been associated with serious human infection. although mechanisms of attachment, persistence on abiotic surfaces, and pathogenesis in a. baumannii have been identified, the genetic mechanisms that explain the emergence of a. baumannii as the most widespread and virulent acinetobacter ...201323365658
identification of opportunistic pathogenic bacteria in drinking water samples of different rural health centers and their clinical impacts on humans.international drinking water quality monitoring programs have been established in order to prevent or to reduce the risk of contracting water-related infections. a survey was performed on groundwater-derived drinking water from 13 different hospitals in the mahabubnagar district. a total of 55 bacterial strains were isolated which belonged to both gram-positive and gram-negative bacteria. all the taxa were identified based on the 16s rrna gene sequence analysis based on which they are phylogenet ...201323862144
synthesis, optimization, and characterization of silver nanoparticles from acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics.the development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. the present study aims to biosynthesize silver nanoparticles (agnps) using a cell-free extract of acinetobacter spp. and evaluate their antibacterial activity.201324235826
defining resistance in acinetobacter calcoaceticus-acinetobacter baumannii complex strains. 201323580585
complete genome sequence of an acinetobacter strain harboring the ndm-1 gene.the ndm-1 gene is a significant public health concern. acinetobacter is one of the most prevalent opportunistic pathogens causing recent nosocomial infections with ndm-1, and drug-resistant strains pose serious threats to public health worldwide. herein, we present the genomic sequence of acinetobacter calcoaceticus subsp. anitratus xm1570, a multidrug-resistant isolate that carries the blandm-1 gene.201323599286
staring at the cold sun: blue light regulation is distributed within the genus acinetobacter.we previously showed that the opportunistic nosocomial pathogen acinetobacter baumannii is able to sense and respond to light via blsa, a bluf (blue-light-sensing using fad)-domain photoreceptor protein. here, we extend our previous studies showing that light regulation is not restricted to a. baumannii, but rather widespread within the genus acinetobacter. first, we found that blue light modulates motility and biofilm formation in many species of the genus, including members of the acinetobacte ...201323358859
real-time fluorescence loop mediated isothermal amplification for the detection of acinetobacter baumannii.detection of acinetobacter baumannii has been relying primarily on bacterial culture that often fails to return useful results in time. although dna-based assays are more sensitive than bacterial culture in detecting the pathogen, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. in addition, these molecular tools require expensive laboratory instruments. therefore, establishing molecular ...201323843955
acinetobacter pittii and acinetobacter nosocomialis among clinical isolates of the acinetobacter calcoaceticus-baumannii complex in sichuan, china.among 82 clinical isolates of the acinetobacter calcoaceticus-baumannii complex recovered in 13 hospitals of sichuan, china, in 2011, 13 were acinetobacter pittii and 2 were acinetobacter nosocomialis. multilocus sequence typing revealed a novel sequence type (st) of a. nosocomialis and 7 novel sts of a. pittii. most isolates were hospital-acquired and colonized in the respiratory tract, while 6 cases with pneumonia due to a. pittii were identified. this study provided a snapshot of the local in ...201323639796
label-free and selective bacteria detection using a film with transferred bacterial configuration.specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface of overoxidized polypyrrole (oppy) film. the recognition of target bacteria was successfully carried out in real time using oppy film in combination with dielectrophoresis. the unique combination of both techniques made the specific detection of template bacilli possible at concentrations as low as 10(3) cfu/ml within 3 min, without any bacterial pretreatment. the observation of ...201323577783
inoculation of phosphate solubilizing bacteria for the improvement of lead accumulation by brassica juncea.two phosphate-solubilizing bacterial strains were isolated and identified as acinetobacter calcoaceticus yc-5a and enterobacter agglomerans kmc-7 based on the 16s rrna gene sequence analysis. a. calcoaceticus yc-5a is less well known as a phosphate-solubilizing plant-associated bacterium. the plant growth-promoting properties of the phosphate-solubilizing bacteria (psb) were characterized in vitro, including their phosphate-solubilizing activities and their capabilities for producing indole-3-ac ...201323530360
emerging threat of multidrug resistant bugs--acinetobacter calcoaceticus baumannii complex and methicillin resistant staphylococcus aureus.infections caused by bacteria such as multidrug resistant (mdr) acinetobacter spp. and methicillin-resistant staphylococcus aureus (mrsa) constitute a worldwide pandemic. without gathering information about these strains, we cannot reduce the morbidity and mortality due to infections caused by these notorious bugs.201323497675
repeated colonization by multi-drug-resistant acinetobacter calcoaceticus-a. baumannii complex and changes in antimicrobial susceptibilities in surgical intensive care units.a nosocomial outbreak of multi-drug-resistant acinetobacter calcoaceticus-a. baumannii (mdr-acb) complex infection occurred in a newly constructed building at a 2,500-bed tertiary medical center in taiwan.201323425282
appraising freeze-drying for storage of bacteria and their ready access in a rapid toxicity assessment assay.direct toxicity assessment (dta) techniques seek to measure the impact of toxic chemicals on biological materials resident in the environment. this study features the use of freeze-dried bacterial cells in combination with a rapid dta analyser, scitox. the effects of three factors-cryoprotectant type, bacterial strain, and storage temperature-were tested in order to validate the shelf life of the freeze-dried cells. three freeze-dried gram-negative bacterial strains, acinetobacter calcoaceticus, ...201323377787
[production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol with organic acids].the effect of fumarate (c4-dicarboxylic acid, a gluconeogenesis precursor) and citrate (a lipid synthesis regulator) on the production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol has been studied. simultaneous addition of fumarate and citrate to concentrations of 0.01-0.02% at the end of the log phase of k-4 growth in a medium with 2 vol% ethanol increases the nominal surfactant concentration by 45-55% in comparison with a culture without organic acids. the increased level ...201323330390
antibacterial and antiplasmodial constituents of beilschmiedia cryptocaryoides.four new beilschmiedic acid derivatives, cryptobeilic acids a-d (1-4), and tsangibeilin b (5) have been isolated from the bark of beilschmiedia cryptocaryoides collected from madagascar. their structures were elucidated using detailed spectroscopic and spectrometric methods. cryptobeilic acid a (1) and tsangibeilin b (5) structures were confirmed by single-crystal x-ray diffraction analysis. compounds 1-5 displayed moderate antibacterial activity against escherichia coli 6r3, acinetobacter calco ...201323320609
[effects of oil-refining microbes (genus acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].the objective of this study was to assess ability of oil-refining bacteria acinetobacter calcoaceticus and a. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. it was found that the cultures infected with a. calcoaceticus showed significantly high frequencies of cytogenetical effects and chromosomal aberrant cells as compared to the intact cultures and cultures infected with a. valentis. the most of chromosomal aberrations, mainly chroma ...201323285752
[intensification of biosurfactant synthesis by acinetobacter calcoaceticus imv b-7241 on a hexadecane–glycerol mixture ]. 201323234072
cloning and characterization of the l-ribose isomerase gene from cellulomonas parahominis mb426.a newly isolated bacterium, cellulomonas parahominis mb426, produced l-ribose isomerase (celri) on a medium containing l-ribose as a sole carbon source. a 32 kda protein isomerizing l-ribose to l-ribulose was purified to homogeneity from this bacterium. a set of degenerated primers were synthesized based on amino acid sequences of the purified celri, and a 747 bp gene encoding celri was cloned, sequenced and overexpressed in escherichia coli. this gene encoded a 249 amino acid protein with a cal ...201323207370
biodegradation of the herbicide propanil, and its 3,4-dichloroaniline by-product in a continuously operated biofilm reactor.the persistence of propanil in soil and aquatic environments along with the possible accumulation of toxic degradation products, such as chloroanilines, is of environmental concern. in this work, a continuous small-scale bioprocess to degrade the herbicide propanil, its main catabolic by-product, 3,4-dichloroaniline (3,4-dca), and the herbicide adjuvants is carried out. a microbial consortium, constituted by nine bacterial genera, was selected. the isolated strains, identified by amplification a ...201323117676
antibiotic resistance patterns of acinetobacter calcoaceticus-a. baumannii complex species from colombian hospitals.only automated phenotypic methods are currently used in colombian hospitals for identifying isolates of the acinetobacter calcoaceticus-a. baumannii complex (acb). the phenotypical similarities in these species mean that they cannot be differentiated by manual or automated methods, thereby leading to their identification as a. baumannii, or acb complex in clinical settings. our objective was to identify to the species level 60 isolates, from four hospitals, evaluate their antibiotic susceptibili ...201323021300
[synthesis of surfactants acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5070 in the medium with glycerol].it was established that glycerol, a byproduct of biodiesel production, may be used as substrate for synthesis of surfactants rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241. maximum indices of surfactants synthesis by the strain imv b-7241 have been fixed, when the medium with glycerol included yeast autolysate and trace elements. it was shown that the surfactants synthesis could be intensified when cultivating a. calcoaceticus imv b-7241 and r. erythropolis imv a ...201322545440
improvement of maldi-tof ms profiling for the differentiation of species within the acinetobacter calcoaceticus-acinetobacter baumannii complex.maldi-tof ms is currently becoming the method of choice for rapid identification of bacterial species in routine diagnostics. yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, namely a. baumannii and acinetobacter nosocomialis. in the present study, we evaluated a protocol which was different from that used in the bruker daltonics identifica ...201324054697
acinetobacter calcoaceticus-acinetobacter baumannii complex is not equal to a. baumannii. 201323295573
molecular epidemiology of acinetobacter baumannii and acinetobacter nosocomialis in germany over a 5-year period (2005-2009).to investigate the species distribution within the acinetobacter calcoaceticus-acinetobacter baumannii complex and the molecular epidemiology of a. baumannii and acinetobacter nosocomialis, 376 acinetobacter isolates were collected prospectively from hospitalized patients at 15 medical centres in germany during three surveillance studies conducted over a 5-year period. species identification was performed by molecular methods. imipenem minimum inhibitory concentrations (mic) were determined by b ...201323034071
highlights on molecular identification of closely related species.the term "complex" emerged in the literature at the beginning of the genomic era associated to taxonomy and grouping organisms that belong to different species but exhibited similar patterns according to their morphological, physiological and/or other phenotypic features. dna-dna hybridization values ~70% and high identity on 16s rrna gene sequences were recommended for species delineation. electrophoretic methods showed in some cases to be useful for species identification and population struct ...201322982158
isolation and characterization of a novel ε-caprolactam-degrading microbe, acinetobacter calcoaceticus, from industrial wastewater by chemostat-enrichment.for the isolation of a ε-caprolactam-degrading microbe from wastewaters of a factory producing caprolactam, we applied a chemostat-enrichment technique with a selective medium containing caprolactam as sole source of carbon and nitrogen. this allowed for the isolation of a novel caprolactam-degrading microbe, identified as acinetobacter calcoaceticus. the strain had a critical tolerance of 19 g caprolactam l(-1) in minimal medium, which is higher than any previously reported caprolactam-degradin ...201323974491
a self-sufficient baeyer-villiger biocatalysis system for the synthesis of ɛ-caprolactone from cyclohexanol.in order to establish a new route for ɛ-caprolactone production from the corresponding cyclohexanol with an internal cofactor recycling for nadph, a recently redesigned thermostable polyol dehydrogenase (pdh) and the cyclohexanone monooxygenase (chmo) from acinetobacter calcoaceticus were combined. first, the expression of pdh could be improved 4.9-fold using e. coli c41 with co-expression of chaperones. both enzymes were also successfully co-immobilized on glutaraldehyde-activated support (reli ...201323931695
[influence of ph on synthesis of acinetobacter calcoaceticus imv b-7241 biosurfactants].synthesis of extracellular metabolites with surface-active and emulsifying properties, ph being maintained at the level of 5.8-8.0, in the process of cultivation of acinetobacter calcoaceticus imv b-7241 in the medium with ethanol (2%, volume part) was investigated. it is established that the neutral value of ph is optimal for synthesis of surface-active substances (sas, biosurfactants) of a. calcoaceticus imv b-7241. the maintenance of ph at the level of 7.0 with the help of koh solution was ac ...201323866584
biodegradation of triton x-100 and its primary metabolites by a bacterial community isolated from activated sludge.a set of studies was carried using a continuous flow biodegradation unit in order to isolate a microbial community capable of efficient and complete utilization of octylphenol ethoxylates from activated sludge. increasing concentrations of triton x-100 (in the range of 1-1000 mg/l) were applied over a time period of 35 days in order to select microorganisms, which exhibit high tolerance towards this surfactant. the fate of the surfactant and its primary degradation products was assessed by hplc/ ...201323770380
isolation and structural characterization of a (kdo-isosteric) d-glycero-α-d-talo-oct-2-ulopyranosidonic acid (ko) interlinking lipid a and core oligosaccharide in the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305.in acinetobacter calcoaceticus nctc 10305 and a. haemolyticus nctc 10305 lipopolysaccharide (lps) a kdo (3-deoxy-d-manno-oct-2-ulosonic acid)-related octulosonic acid (ko) interlinks the lipid a with the core-oligosaccharide. this ko replaces the first kdo (kdo(i)) attached to the lipid a backbone in the lps. the only structural difference between kdo and ko is the 3-hydroxylation. after the discovery of the final step in ko-biosynthesis it is now generally accepted that ko is structurally relat ...201323743267
development of a whole-cell biocatalyst with nadph regeneration system for biosulfoxidation.a formate dehydrogenase gene (fdh) originated from candida boidinii was co-expressed in e. coli bl21 (de3) with the cyclohexanone monooxygenase gene (chmo) cloned from acinetobacter calcoaceticus ncimb 9871. the co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide (pmso) from thioanisole (pms) and the reaction conditions were investigated. when the initial concentration of pms was 20 mm, the specific productivity of pmso in this system was 2 ...201323729190
[effect of univalent cations on synthesis of surfactants by acinetobacter calcoaceticus imv b-7241].the effect of univalent cations on activity of key enzymes of c2-metabolism has been investigated in the producer of biosurfactants, acinetibacter calcoaceticus imv b-7241 grown on ethanol. it was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (nadp-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (phep)-carboxikinase), while ammonium cati ...201323720959
antimicrobial susceptibility pattern in nosocomial infections caused by acinetobacter species in asir region, saudi arabia.this study aimed at evaluating the sensitivity of antibiotics towards nosocomial infections caused by acinetobacter species. the study took place during the period dec. 2011- dec. 2012 at assir central hospital in collaboration with the department of microbiology, college of medicine, king khalid university, abha. a prospective study involving 150 patients presented with nosocomial infections due to acinetobacter species detected by bacteriological tests; direct microscopy, culture in blood agar ...201324498790
identification of genetic recombination between acinetobacter species based on multilocus sequence analysis.during multilocus sequence analysis of acinetobacter calcoaceticus-acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 acinetobacter species. while 3 isolates belonged to a. nosocomialis based on 16s rrna, gyrb, fusa, gdhb, and rplb gene sequences, they showed close relationships with acinetobacter genomic species 'close to 13tu' in rpob, reca, cpn60, rpod, and glta gene trees.201222595610
clinical characteristics of tertiary hospital patients from whom acinetobacter calcoaceticus-acinetobacter baumannii complex strains were isolated.acinetobacter baumannii is a worldwide nosocomial pathogen that has become increasingly common over the past few decades, and strains of multidrug-resistant a. baumannii have been increasing. the aim of this study was to assess the clinical characteristics of a. calcoaceticus-a. baumannii complex (acb complex) strains and to determine the risk factors of this infection.201222214623
cellular fatty acids as chemical markers for differentiation of acinetobacter baumannii and acinetobacter calcoaceticus.gas chromatography (gc) was used to investigate the cellular fatty acid (cfa) composition of 141 acinetobacter baumannii and 32 a. calcoaceticus isolates from different locations in china and to find chemical markers to differentiate these two closely related bacteria.201223228842
comparative analysis of fecal microflora of healthy full-term indian infants born with different methods of delivery (vaginal vs cesarean): acinetobacter sp. prevalence in vaginally born infants.in this study fecal microflora of human infants born through vaginal delivery (vb) and through cesarean section (cb) were investigated using culture-independent 16s rdna cloning and sequencing approach. the results obtained clearly revealed that fecal microbiota of vb infants distinctly differ from those in their counterpart cb infants. the intestinal microbiota of infants delivered by cesarean section appears to be more diverse, in terms of bacteria species, than the microbiota of vaginally del ...201223151789
domiciliary cockroaches found in restaurants in five zones of kuala lumpur federal territory, peninsular malaysia.the following domiciliary cockroaches were collected from restaurants in five zones of kuala lumpur federal territory, malaysia using 1l glass beaker traps baited with ground mouse-pellets: periplaneta americana (linnaeus) (n = 820), periplaneta brunnea burmeister (n = 46), blattella germanica (linnaeus) (n = 12504), supella longipalpa (fabricius) (n = 321), symploce pallens stephens (n = 29) and neostylopyga rhombifolia (stoll) (n = 5). the following bacteria were isolated from 10 cockroach spe ...201222543619
infections due to different genospecies of the acinetobacter calcoaceticus-a baumannii complex. 201222526214
vertical immobilization of viable bacilliform bacteria into polypyrrole films.we have successfully developed a novel technique for inserting viable bacilliform bacteria into polypyrrole films. all of the five different bacterial cells (pseudomonas aeruginosa, acinetobacter calcoaceticus, serratia marcescens, bacillus subtilis and escherichia coli) studied in this work were inserted normal to the film surface, and the viability of p. aeruginosa was unaffected by this immobilization procedure. it was also found that the polypyrrole layer was important to keep the cells aliv ...201222498456
multitrophic interaction in the rhizosphere of maize: root feeding of western corn rootworm larvae alters the microbial community composition.larvae of the western corn rootworm (wcr) feeding on maize roots cause heavy economical losses in the us and in europe. new or adapted pest management strategies urgently require a better understanding of the multitrophic interaction in the rhizosphere. this study aimed to investigate the effect of wcr root feeding on the microbial communities colonizing the maize rhizosphere.201222629377
a five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by acinetobacter radioresistens sh164.acinetobacter calcoaceticus sw1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (tauxy). blast searches of newly available genome sequences using the tauxy sequences revealed a 5-gene cluster, taurxypi, in acinetobacter radioresistens sh164. we hypothesized that tauxypi (hmpref0018_00717-hmpref0018_00720) encodes proteins that are orthologs of the undefined pathway from st ...201222588221
acinetobacter calcoaceticus-baumannii complex strains induce caspase-dependent and caspase-independent death of human epithelial cells.we investigated interactions of human isolates of acinetobacter calcoaceticus-baumannii complex strains with epithelial cells. the results showed that bacterial contact with the cells as well as adhesion and invasion were required for induction of cytotoxicity. the infected cells revealed hallmarks of apoptosis characterized by cell shrinking, condensed chromatin, and internucleosomal fragmentation of nuclear dna. the highest apoptotic index was observed for 4 of 10 a. calcoaceticus and 4 of 7 a ...201222684803
Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea.Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ...201222130193
identification of acinetobacter species: is bruker biotyper maldi-tof mass spectrometry a good alternative to molecular techniques?acinetobacter spp. has become a leading cause of nosocomial infection in recent years. phenotypic similarities between the species in the genus have made it difficult to identify them clearly using routine diagnostic methods. consequently, more relevant species have been grouped together as acinetobacter calcoaceticus-acinetobacter baumannii complex (a. baumannii, a. calcoaceticus, acinetobacter genospecies 3 and a. genospecies 13tu). however, there are other species that may also have clinical ...201222266021
characterization of rhamnolipids produced by non-pathogenic acinetobacter and enterobacter bacteria.rhamnolipid production by two non-pathogenic bacterial strains acinetobacter calcoaceticus and enterobacter asburiae, and established rhamnolipid producer pseudomonas aeruginosa was investigated. rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homolo ...201223313768
comparison of genospecies and antimicrobial resistance profiles of isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from various clinical specimens.this study was conducted to compare the prevalences of antimicrobial resistance profiles of clinical isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from sterile and nonsterile sites and to further study the relationship of antimicrobial resistance profiles and genospecies by amplified rrna gene restriction analysis (ardra). a total of 1,381 isolates were tested with 12 different antibiotics to show their antimicrobial susceptibility profiles. a total of 205 clinical ...201223006758
recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii complex.this study investigated the clinical and microbiological characteristics of patients with recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex at a medical center. all acb complex isolates associated with recurrent bacteremia were identified to the genomic species level using a 16s-23s rrna gene intergenic spacer sequence-based method. genotypes were determined by the random amplified polymorphic dna patterns generated by arbitrarily primed pcr and ...201222760035
expression of the resistance-nodulation-cell division pump adeijk in acinetobacter baumannii is regulated by aden, a tetr-type regulator.resistance-nodulation-division efflux system adeijk contributes to intrinsic resistance to various drug classes in acinetobacter baumannii. by whole-genome sequencing, we have identified in clinical isolate bm4587 the aden gene, located 813 kbp upstream from adeijk, which encodes a tetr transcriptional regulator. in one-step mutant bm4666 overexpressing adeijk, the deletion of cytosine 582 (c(582)) in the 3' portion of this gene was responsible for a frameshift mutation resulting in the deletion ...201222371895
isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating pvc materials.waste materials containing di-(2-ethylhexyl) phthalate (dehp), a suspected endocrine disruptor and reasonably anticipated human carcinogen, are typically disposed of in landfills. despite this, very few studies had been conducted to isolate and identify dehp-degrading bacteria in landfill leachate. therefore, this study was conducted to isolate and characterize bacteria in landfill leachate growing on dehp as the sole carbon source and deteriorating pvc materials. four strains lhm1, lhm2, lhm3 a ...201222934997
screening and characterization of a novel alkaline lipase from acinetobacter calcoaceticus 1-7 isolated from bohai bay in china for detergent formulation.a novel alkaline lipase-producing strain 1-7 identified as acinetobacter calcoaceticus was isolated from soil samples collected from bohai bay, china, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. the lipase from strain 1-7 showed the maximum activity at ph 9.0 under 40 °c. one interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. it is also stable at a broad range of phs from 4.0 to ...201224031813
reductive dechlorination of methoxychlor by bacterial species of environmental origin: evidence for primary biodegradation of methoxychlor in submerged environments.methoxychlor [1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane] is an organochlorine insecticide that undergoes dechlorination in natural submerged environments. we investigated the ability to dechlorinate this compound in seven environmental bacterial species ( aeromonas hydrophila , enterobacter amnigenus , klebsiella terrigena , bacillus subtilis , achromobacter xylosoxidans , acinetobacter calcoaceticus , and mycobacterium obuense ) and the enteric bacterium escherichia coli as a positive cont ...201222292429
changes in antimicrobial susceptibility and major clones of acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in korea over 7 years.acinetobacter species have emerged as opportunistic nosocomial pathogens in intensive care units. epidemic spread and outbreaks of multidrug-resistant or carbapenem-resistant acinetobacter baumannii infections have been described worldwide. species distribution, antimicrobial resistance and genotypes were investigated for acinetobacter species isolates collected from a single institution in korea over 7 years. two hundred and eighty-seven acinetobacter species isolates were collected from patien ...201221852525
Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives.The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871, were investigated. Baeyer-Villiger oxygenation of 2-(2'-acetoxyethyl) cyclohexanone (MW 170 Da) into R-7-(2'-acetoxyethyl)-2-oxepanone was used as a model reaction. The whole-cell biotransformation followed Michaelis-Menten kinetics. The V (max) and K (S) va ...201121909677
diverse dna damage responses in acinetobacter include the capacity for dna damage-induced mutagenesis in the opportunistic pathogens acinetobacter baumannii and acinetobacter ursingii.error-prone and error-free dna damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics, or radiation sources were surveyed across the acinetobacter genus. the error prone sos mutagenesis response occurs when dna damage induces a cell's umudc- or dinp-encoded error-prone polymerases. the model acinetobacter baylyi strain adp1 possesses an unusual, regulatory umud allele (umudab) with an extended 5' region, and only incomplete fragments of umuc. d ...201122117008
recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing pseudomonas putida strain ncimb 10007.abstract: three different baeyer-villiger monooxygenases (bvmos) were reported to be involved in the camphor metabolism by pseudomonas putida ncimb 10007. during (+)-camphor degradation, 2,5-diketocamphane is formed serving as substrate for the 2,5-diketocamphane 1,2-monooxygenase. this enzyme is encoded on the cam plasmid and depends on the cofactors fmn and nadh and hence belongs to the group of type ii bvmos. we have cloned and recombinantly expressed the oxygenating subunit of the 2,5-diketo ...201121906366
comparative analysis of the complete genome of an acinetobacter calcoaceticus strain adapted to a phenol-polluted environment.the complete genome sequence of acinetobacter calcoaceticus phea-2, a non-pathogenic phenol-degrading bacterium previously isolated from industrial wastewater of an oil refinery in china, has been established. this is the first sequence of an a. calcoaceticus strain. we report here a comparative genomic analysis of phea-2 with two other acinetobacter species having different lifestyles, acinetobacter baumannii aye, a pathogenic human-adapted strain, and acinetobacter baylyi adp1, a soil-living s ...201122027104
multifunctional acyltransferases from tetrahymena thermophila.multifunctional acyltransferases are able to catalyze the esterification of various acyl-acceptors with activated fatty acids. here we describe the identification of four proteins from tetrahymena thermophila that share certain properties with mammalian acyltransferases regarding their predicted transmembrane structure, their molecular mass and the typical acyltransferase motif. expression of the tetrahymena sequences results in production of triacylglycerols and wax esters in recombinant yeast ...201122160552
rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis.we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ...201121289149
first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae.we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ...201121307209
genotypic and phenotypic characterization of the acinetobacter calcoaceticus-acinetobacter baumannii complex with the proposal of acinetobacter pittii sp. nov. (formerly acinetobacter genomic species 3) and acinetobacter nosocomialis sp. nov. (formerly acinetobacter genomic species 13tu).acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13tu are increasingly recognized as clinically important taxa within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. to define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the acb complex. all strains were characterized by aflp analysis, amplified rdna restriction analysis and nutritional or physiological testing, while selected strains were studied by ...201121320596
characterization of n-acylhomoserine lactone-degrading bacteria associated with the zingiber officinale (ginger) rhizosphere: co-existence of quorum quenching and quorum sensing in acinetobacter and burkholderia.cell-to-cell communication (quorum sensing (qs)) co-ordinates bacterial behaviour at a population level. consequently the behaviour of a natural multi-species community is likely to depend at least in part on co-existing qs and quorum quenching (qq) activities. here we sought to discover novel n-acylhomoserine lactone (ahl)-dependent qs and qq strains by investigating a bacterial community associated with the rhizosphere of ginger (zingiber officinale) growing in the malaysian rainforest.201121385437
species identification and molecular characterization of acinetobacter spp. blood culture isolates from norway.the study investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates of acinetobacter species collected between 2005 and 2007 throughout norway.201121393175
cyclopropanation of membrane unsaturated fatty acids is not essential to the acid stress response of lactococcus lactis subsp. cremoris.cyclopropane fatty acids (cfas) are synthetized in situ by the transfer of a methylene group from s-adenosyl-l-methionine to a double bond of unsaturated fatty acid chains of membrane phospholipids. this conversion, catalyzed by the cfa synthase enzyme, occurs in many bacteria and is recognized to play a key role in the adaptation of bacteria in response to a drastic perturbation of the environment. the role of cfas in the acid tolerance response was investigated in the lactic acid bacterium lac ...201121421775
polymerase chain reaction assay for the detection of acinetobacter baumannii in endotracheal aspirates from patients in the intensive care unit.we aim to evaluate the efficacy of polymerase chain reaction (pcr) to detect acinetobacter baumannii in endotracheal aspirates.201121439512
genome sequence of acinetobacter calcoaceticus phea-2, isolated from industry wastewater.genome analysis of acinetobacter calcoaceticus phea-2 was undertaken because of the importance of this bacterium for bioremediation of phenol-polluted water and because of the close phylogenetic relationship of this species with the human pathogen acinetobacter baumannii. to our knowledge, this is the first strain of a. calcoaceticus whose genome has been sequenced.201121441526
novel regulator mphx represses activation of phenol hydroxylase genes caused by a xylr/dmpr-type regulator mphr in acinetobacter calcoaceticus.acinetobacter calcoaceticus phea-2 utilizes phenol as its sole carbon and energy source and has a multi-component phenol hydroxylase-encoding gene operon (mphklmnop) for phenol degradation. two additional genes, mphr and mphx, were found upstream and downstream of mphklmnop, respectively. the mphr gene encodes a xylr/dmpr-type regulator-like protein and is transcribed in the opposite direction to mphklmnop. the mphx gene is transcribed in the same direction as mphklmnop and encodes a protein wit ...201121455294
microbiology of animal bite wound infections.the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ...201121482724
antibacterial activity in spices and local medicinal plants against clinical isolates of karachi, pakistan.context: development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. twenty-seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study. eleven plants chosen from these 27 are used as spices in local cuisine. objective: evaluation of the effectiveness of some medicinal plant extracts a ...201121501041
synergistic effect of thermophilic temperature and biosurfactant produced by acinetobacter calcoaceticus bu03 on the biodegradation of phenanthrene in bioslurry system.this study aimed at investigating the synergistic effect of temperature and biosurfactant on the biodegradation of phenanthrene in bioslurry. bench-scale bioslurry experiments were conducted at 25 and 55°c. the desorption rate coefficients of phenanthrene (k(des)) obtained using the pseudo-first order model were 0.0026 and 0.0035kgmg(-1)h(-1) at 25 and 55°c, respectively. addition of 1500mgl(-1) biosurfactant, produced by acinetobacter calcoaceticus bu03, marginally increased the k(des) at 25°c ...201121530078
biofilm formation and adherence characteristics of an elizabethkingia meningoseptica isolate from oreochromis mossambicus.abstract: background: elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. their ability to exist as biofilm structures has been alluded to but not extensively investigated. methods: the ability of elizabethkingia meningoseptica isolate ch2b from freshwater tilapia (oreochromis mossambicus) and e. meningoseptica strain nctc 10016t to adhere to abiotic surfaces was investigated using microtiter ...201121545730
a shigella boydii bacteriophage which resembles salmonella phage vii.abstract: background: lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications. results: the lytic bacteriophage, phisbom-ag3, targets the important foodborne pathogen, shigella. it is morphologically similar to phage vii of salmonella enterica serovar typhi and a series of phages of acinetobacter calcoaceticus and rhizobium ...201121595934
genomic comparison of multi-drug resistant invasive and colonizing acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity.abstract: background: acinetobacter baumannii has recently emerged as a significant global pathogen, with a surprisingly rapid acquisition of antibiotic resistance and spread within hospitals and health care institutions. this study examines the genomic content of three a. baumannii strains isolated from different body sites. isolates from blood, peri-anal, and wound sources were examined in an attempt to identify genetic features that could be correlated to each isolation source. results: pulse ...201121639920
the acinetobacter baumannii reca protein in the repair of dna damage, antimicrobial resistance, general stress response, and virulence.reca is the major enzyme involved in homologous recombination and plays a central role in sos mutagenesis. in acinetobacter spp., including a. baumannii, a multidrug-resistant bacterium responsible for nosocomial infections worldwide, dna repair responses differ in many ways from those of other bacterial species. in this work, the function of a. baumannii reca was examined by constructing a reca mutant. alteration of this single gene had a pleiotropic effect, showing the involvement of reca in d ...201121642465
acinetobacter baumannii and acinetobacter genospecies 13tu and 3 bacteraemia: comparison of clinical features, prognostic factors and outcomes.to investigate the clinical impact of different genospecies of the acinetobacter calcoaceticus-acinetobacter baumannii complex (acb complex; a. baumannii, acinetobacter gen. sp. 13tu and acinetobacter gen. sp. 3) on the severity of bacteraemia.201121653602
efficient direct electron transfer of pqq-glucose dehydrogenase on carbon cryogel electrodes at neutral ph.we present a comprehensive study of the direct electron transfer reaction of soluble pqq-gdh from acinetobacter calcoaceticus. wild-type pqq-sgdh nonspecifically adsorbed on carbon cryogel electrodes retained its enzymatic activity for glucose and maltose oxidation at ph 7.2 and 37 ┬░c. the cyclic voltammograms in the absence of enzymatic substrate showed 2 redox peaks that suggest a two-step, one-electron oxidation/reduction of pqq. calibration curves showed a linear amperometric response for a ...201121662989
microorganisms from aphid honeydew attract and enhance the efficacy of natural enemies.aphids are one of the most serious pests of crops worldwide, causing major yield and economic losses. to control aphids, natural enemies could be an option but their efficacy is sometimes limited by their dispersal in natural environment. here we report the first isolation of a bacterium from the pea aphid acyrthosiphon pisum honeydew, staphylococcus sciuri, which acts as a kairomone enhancing the efficiency of aphid natural enemies. our findings represent the first case of a host-associated bac ...201121673669
[effect of biosurfactants acinetobacter calcoaceticus k-4 and rhodococcus erythropolis ek-1 on some microorganisms].it has been established that the preparations of biosurfactants rhodococcus erythropolis ek-1 (0.61 -2.1 mg/ml) and acinetobacter calcoaceticus k-4 (0.15 - 0.22 mg/ml) in a form of supernatants of the cultural liquid show antimicrobial effect in respect of a number of microorganisms (bacillus subtilis bt-2, escherichia coli iem-1, candida tropicalis bt-5, candida albicans d6, candida utilis bvc-65, saccharomyces cerevisiae ob-3). no inhibiting effect of biosurfactant preparations of r. erythropo ...201121809683
genomic analysis of the multi-drug resistant acinetobacter baumannii strain mdr-zj06 widely spread in china.we previously reported that the multi-drug resistant (mdr) acinetobacter baumannii strain mdr-zj06 belonging to european clone ii was widely spread in china. in this study, we report the whole genome sequence of this clinically important strain. a 38.6 kb abar-type genomic resistance island (abar11) was identified in mdr-zj06. abar11 has a similar structure to the resistance islands found in a. baumannii strains aye and ab0057, but it only contained few antibiotic resistant genes. the region of ...201121788470
biofilm formation and microbial activity in a biofilter system in the presence of mtbe, etbe and tame.emerging water contaminants derived from unleaded gasoline such as methyl tert-butyl ether (mtbe), ethyl tert-butyl ether (etbe) and tert-amyl methyl ether (tame), are in need of effective bioremediation technologies for restoring water resources. in order to design the conditions of a future groundwater bioremediating biofilter, this work assesses the potential use of acinetobacter calcoaceticus m10, rhodococcus ruber e10 and gordonia amicalis t3 for the removal of mtbe, etbe and tame in consor ...201121774959
a clpr-like regulator specifically recognizes a reca-independent promoter motif and broadly regulates expression of dna damage inducible genes in mycobacteria.the reca-dependent dna damage response pathway (sos response) appears to be the major dna repair mechanism in most bacteria, but it has been suggested that a reca-independent mechanism is responsible for controlling expression of most damage-inducible dna repair genes in mycobacterium tuberculosis. the specific reparative responses and molecular mediators involved in the dna repair mechanism remain largely unclear in this pathogen and its related species. in this study, a mycobacterial clpr-like ...201121771781
horizontal gene transfer/assortative recombination within the acinetobacter baumannii clinical population provides genetic diversity at the single caro gene encoding a major outer membrane protein channel.we described previously the presence in acinetobacter baumannii of a novel outer membrane (om) protein, caro, which functions as an l-ornithine om channel and whose loss was concomitant with increased carbapenem resistance among clonally related nosocomial isolates of this opportunistic pathogen. here, we describe the existence of extensive genetic diversity at the caro gene within the a. baumannii clinical population. the systematic analysis of caro sequences from a. baumannii isolates obtained ...201121764928
biological improvement on combined mycelial pellet for aniline treatment by tourmaline in sbr process.as a biomass carrier, mycelial pellet of aspergillus niger y3 was used to immobilize the aniline-degrading bacterium acinetobacter calcoaceticus jh-9 and the mix culture of the cod rapid degrading bacteria in this study. tourmaline was added to this system in order to improve the aniline removal performance using combined mycelial pellet. flask experiments were performed to investigate the promotion mechanism. the results showed that the start-up time was shorted from 7 cycles to only 1 cycle. t ...201121764303
identification and characterization of a new true lipase isolated through metagenomic approach.abstract: background: metagenomics, the application of molecular genomics to consortia of non-cultivated microbes, has the potential to have a substantial impact on the search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, ec 3.1.1.1) and lipases (triacylglycerol lipases, ec 3.1.1.3). in the current work, a novel lipase gene was identified from a fosmid metagenomic library constructed with the "prokaryotic-enriched" dna from a fat-contaminated soil collected from a wa ...201121762508
amylolytic microorganism from s+úo paulo zoo composting: isolation, identification, and amylase production.composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. this transformation is the result of the microorganism action, converting complex carbon sources into energy. enzymes that are exported by the microorganisms to the surrounding environment mediate this process. the aiming of the present work is to prospect the compost produced by the organic composting unit (ocu) of the funda+º+úo parque zool+¦gic ...201121845217
emergence of tigecycline & colistin resistant acinetobacter baumanii in patients with complicated urinary tract infections in north india. 201121727671
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