TitleAbstractYear(sorted ascending)
chronic haematuria caused by acinetobacter calcoaceticus. 1978567878
[acinetobacter calcoaceticus as the possible cause of bronchopneumonia in rabbits]. 1978352659
evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli.the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ...1978353066
evaluation of the api 20e system for identification of nonfermentative gram-negative bacteria.the api 20e system for enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the api 20e data base. the results of 16 tests common to both systems were in close agreement. the api 20e system correctly identified 71 (43%) of the 165 organisms inclu ...1978353067
nosocomial respiratory tract infection and colonization with acinetobacter calcoaceticus. epidemiologic characteristics.nosocomial respiratory tract infection with acinetobacter calcoaceticus occurs frequently in many hospitals. an outbreak of respiratory tract infections in an intensive care unit provided an opportunity to study clinical and epidemiologic characteristics of such infections. retrospective studies demonstrated that a. calcoaceticus in sputum was significantly associated with endotracheal intubation (p = 0.03) and continuous positive pressure ventilation (p less than 0.02). after control measures h ...1978362918
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
[kinetic and regulatory properties of malate dehydrogenase from acinetobacter calcoaceticus]. 1978216197
microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes.resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests.1978100053
[oxidation of homologous aliphatic aldehydes by membrane-bound and solubilized aldehyde dehydrogenase from acinetobacter calcoaceticus]. 197832669
acinetobacter calcoaceticus: common nosocomial organism, uncommon pathogen. 197810236786
[properties of rubredoxin reductase from the alkane-assimilating bacterium acinetobacter calcoaceticus]. 197944771
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. 197994750
nadh and nadph-viologen reductases from acinetobacter calcoaceticus.three pyridine nucleotide-dependent diaphorases have been isolated from acinetobacter calcoaceticus cells and partially characterized. two of them, with molecular weights of 165,000 and 57,000, utilize nadph as electron donor whereas the third one (mw = 57,000) is specific for nadh. oxidized viologen dyes, flavin nucleotides, dichlorophenol indophenol and ferricyanide can act with efficiency as acceptors in the reaction mediated by these diaphorases. the diaphorase activities have been character ...1979223203
identification of gram-negative non-fermenters and oxidase-positive fermenters by the oxi/ferm tube.since the recent introduction of the roche oxi/ferm tube to the uk two identification schemes have been developed by the manufacturer for use with the kit. we evaluated the success of these two schemes in identifying 222 predominantly culture collection strains belonging to 45 taxa of non-fermenters and nine taxa of oxidase-positive fermenters. the strains were chosen to represent all the taxa included in the two identification schemes developed by the manufacturer and we have therefore been abl ...1979372251
acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem.acinetobacter calcoaceticus var anitratus is an increasing nosocomial problem in some institutions. duirng a recent 16-month period, we observed 15 patients with blood isolates of this organism. thirteen of these patients acquired the organism from the hospital environment. eleven of these represented real disease and concurrent growth of the organism from another site was predictive of infection (p less than 0.03). a review of laboratory records showed that there was a three-fold increase in no ...1979426000
aminoglycoside-modifying enzymes among clinical isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola): explanation for high-level aminoglycoside resistance.acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) is an important cause of nosocomial infection in our hospital where a. calcoaceticus subsp. anitratus is the most frequently isolated gram-negative species resistant to one or more of the aminoglycoside antibiotics. of 167 strains tested for susceptibility to aminoglycosides, only 6 strains were found that were resistant to >/=128 mug of kanamycin per ml; all others were susceptible to </=32 mug/ml. five of these six strains were ...1979426512
acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. 1979448188
ventriculitis from acinetobacter calcoaceticus variant anitratus. 1979448387
community-acquired acinetobacter pneumonia.acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. in this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. acinetobacter pneumonia occurs in older persons w ...1979463916
a hand infection from a. calcoaceticus (m. polymorpha).acinetobacter calcoaceticus (mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. in a joint infection, delay in identification or appropriate treatment can result in added morbidity. an a. calcoaceticus (mima) infection in the hand seems not to have been previously reported. the present case involving a finger joint infection illustrates the pathogenicity of a. calcoaceticus (mima) and problems of treatmen ...1979477074
glucose dehydrogenase from acinetobacter calcoaceticus: a 'quinoprotein'. 1979520586
serological cross-reactions between acinetobacter calcoaceticus and chlamydiae.a cross-reaction between acinetobacter calcoaceticus and chlamydiae is described. a water-soluble, heat stable, non-dialyzable antigen was extracted from acinetobacter species by boiling. this antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies. hyperimmune antisera to the extracted antigen, or to suspensions of live acinetobacters, also reacted ...1979521480
in vitro activity of three tetracycline antibiotics against acinetobacter calcoaceticus subsp. anitratus.the in vitro activity of three tetracycline antibiotics against 127 strains of acinetobacter calcoaceticus (herella vaginicola) were compared. almost all strains were susceptible to minocycline and doxycycline, whereas most strains were resistant to tetracycline.1979526013
[structural features of acinetobacter calcoaceticus k-9 cells assimilating ethanol at different rates of medium dilution]. 1979530115
[uptake of organic acids by acinetobacter calcoaceticus]. 1979543155
[a case of meningitis due to acinetobacter calcoaceticus].meningitis due to acinetobacter calcoaceticus is rare. these rare bacteria were isolated from culture fluid in patient resembling to neisseria meningitis with clinical and laboratory findings. the patient was treated with sensitive antibiotic.1979555785
origins of metabolic diversity: evolutionary divergence by sequence repetition.recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ...1979291059
meningitis due to acinetobacter calcoaceticus variant anitratus. 19806182240
[quantitative immunological method for determining rubredoxin in crude extracts of acinetobacter calcoaceticus]. 19806246689
intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from azotobacter vinelandii.protocatechuate 3,4-dioxygenase (ec was purified to homogeneity from extracts of azotobacter vinelandii. the molecular weight of the oligomeric protein was estimated to be 510 000 by gel filtration and 480 000 by ultracentrifugation. the oligomer appears to be formed by association of equal amounts of nonidentical subunits which were estimated by sodium dodecyl sulfate gel electrophoresis to have respective molecular weights of 23 300 and 25 250. ten gram-atoms of iron was associated w ...19806766312
antimicrobial susceptibility of acinetobacter calcoaceticus, serratia marcescens, pseudomonas fluorescens and pseudomonas maltophilia to three novel cephalosporin-like antibiotics. 19806777362
selective nonroutine microbial surveillance of in-use hospital nebulizers by aerosol entrapment and direct sampling analyses of solutions in reservoirs.effluent aerosols and liquid reservoir samples from 255 in-use hospital nebulization devices were analyzed by aero-test samplers (olympic corp.) and direct dilution procedures (0.1-, 0.01-, and 0.001-ml plated samples). thirty-five percent (89 of 255) of the in-use hospital reservoir samples were positive by direct dilution, and 24% (61 of 255) were positive by aero- test samplers. acinetobacter calcoaceticus var. anitratus was found either alone or in association with pseudomonas spp. in 50% of ...19806785304
evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures.a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ...19806999020
studies on glucose nonfermentative gram-negative bacilli. ii. transferable drug resistance in acinetobacter calcoaceticus. 19807000977
solubilization and stabilization of l(+)-mandelate dehydrogenase from acinetobacter calcoaceticus. 19807004952
restriction mediated by pav2 affects the transfer of plasmids in acinetobacter calcoaceticus.the naturally occurring plasmid pav2 restricts the entry of the p class plasmid rp4 and the w class plasmids r388 and s-a into acinetobacter calcoaceticus strain ebf65/65 from escherichia coli. the w class plasmids only transfer from e. coli into pav2-strains. plasmid rp4 is modified in the presence of pav2 such that it is no longer restricted on entry into pav2 recipients of strain ebf65/65.19807021765
behaviour of bacteriophage mu in acinetobacter calcoaceticus ebf65/65.transfer of rp4::mu plasmids from escherichia coli k12 to acinetobacter calcoaceticus ebf65/65 was very inefficient compared with rp4 and was only detectable to strains of ebf65/65 lacking pav2, a cryptic plasmid thought to code for a restriction/modification system. rp4::mu-derived plasmids transferred from pav2-strains of ebf65/65 back to e. coli k12 were found to carry defective prophages which had lost the ability to produce detectable phage particles. re-transfer of these defective plasmids ...19807021766
a common source outbreak of acinetobacter pulmonary infections traced to wright respirometers.over a 30-day period, acinetobacter calcoaceticus var. antiratus was the responsible pathogen for hospital-acquired pneumonia in 10 patients, and resulted in the colonization of the upper respiratory tract in an additional 9 patients. wright respirometers contaminated by this organism were shown to be the common source for the outbreak as indicated by the recovery of a single serotype (8j), the inability to recover acinetobacter from any other environmental source, and the demonstration that moi ...19806930637
purification and characterization of rhodanese from acinetobacter calcoaceticus.rhodanese (thiosulfate : cyanide sulfur transferase, ec 2,8,1,1) was found to be contitutively present as an intracellular enzyme in acinetobacter calcoaceticus. the soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, cm-sephadex batchwise separation, qae-50 ion exchange chromatography, and socrose density gradient ultracentrifugation. the enxyme had a molecular weight of approximately 35000 with a ph optimum of 8-8.5. activity was subs ...19806931629
infective endocarditis due to acinetobacter calcoaceticus. 19807205689
influence of growth phase and carbon source on the content of rubredoxin in acinetobacter calcoaceticus.the rubredoxin content of acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, l-malate) and on the growth phase was studied by means of a radioimmunoassay. the method used was specific for rubredoxin and acinetobacter calcoaceticus. the formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. after growth of acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not ...19807212930
[epidemiology of acinetobacter calcoaceticus]. 19807243591
evidence of plasmid-mediated production of aminoglycoside-modifying enzymes not previously described in acinetobacter.two blood culture isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) were recently observed to be unusually resistant to aminoglycosides. each strain was found to contain aminoglycoside-modifying enzymes which have not been described previously in this species, including 2"-adenylyltransferase, 3"-adenylyltransferase, 3'-phosphotransferase-iii, and 3-acetyltransferase. treatment of one strain (h-s) with novobiocin led to a loss of resistance to multiple antimicrobial ...19807352749
regulation of phosphoenolpyruvate carboxylase from acinetobacter calcoaceticus by nucleotides. 19807353640
naturally occurring plasmids in acinetobacter calcoaceticus: a p class r factor of restricted host range.a naturally occurring transmissible plasmid, designated pav1, has been isolated in acinetobacter calcoaceticus. it specifies resistance to sulphonamides and is capable of mobilizing two non-transmissible resistance determinants for tetracycline and neomycin, respectively, within strains of a. calcoaceticus. it is incompatible with the p class r factors rp4 and r751 in a. calcoaceticus. on this basis we conclude that pav1 is a member of the p incompatibility group. however, unlike most other p gr ...19807365457
repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida.muconolactone delta-isomerase (ec and beta-ketoadipate enol-lactone hydrolase (ec mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ...19807391022
evolutionary divergence of co-selected beta-ketoadipate enol-lactone hydrolases in acinetobacter calcoaceticus.muconolactone isomerase (ec and beta-ketoadipate enol-lactone hydrolase (ec mediate consecutive catabolic steps in bacteria. separately inducible beta-ketoadipate enol-lactone hydrolases i and ii are formed in representatives of acinetobacter calcoaceticus. when subjected to deae-cellulose chromatography, acinetobacter enol-lactone hydrolase i displays heterogeneous behavior which, in whole or in part, appears to be due to modifications of sulfhydryl groups in the protein; the ...19807391023
homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases.gamma-carboxymuconolactone decarobxylase (ec and muconolactone isomerase (ec mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ...19807391024
pyrogenic reactions after inadvertent infusion of endotoxin during cardiac april 1976 the attack rate of chills and fever with or without falls in blood pressure increased in association with cardiac catheterizations. fevers were associated with coronary angiography and right and left heart catheterizations. blood cultures were negative, and reactions did not correlate with amounts of contrast materials infused or with procedures done by a single operator. significant numbers of acinetobacter calcoaceticus (var. anitratus) and a pseudomonas species were cultured fro ...19807396310
gene transfer in acinetobacter calcoaceticus: fertility variants of the sex factor pav1.the naturally occurring transmissible plasmid pav1 mediates chromosome transfer and can exhibit two distinct levels of transmissibility in acinetobacter calcoaceticus strain ebf65/65. the two states of pav1 have been arbitrarily designated pav1a (low frequency variant) and pav1b (high frequency variant). both variants have the same incompatibility and host range properties and each mobilizes two non-transmissible resistance determinants for tetracycline and neomycin. sex factor activity has been ...19807411116
symptomatic acinetobacter calcoaceticus peritonitis. "a complication of peritoneal dialysis".acinetobacter calcoaceticus peritonitis was seen during the course of peritoneal dialysis in two patients with end-stage kidney disease within a 3-month period. in one patient, the isolate was a. calcoaceticus varient anitratus (formerly herellea vaginicola) and in the other it was a. calcoaceticus lwoffi (formerly mima polymorpha). both were successfully treated with continuous peritoneal antibiotic lavage.19807440841
an outbreak of acinetobacter infection associated with the use of a ventilator spirometer.although respiratory therapy equipment is a well-known source of nosocomial infection, ventilator spirometers have not been previously implicated. we report 17 acinetobacter calcoaceticus variety anitratus infections traced to contaminated spirometers. isolates from infected patients were recovered from urine, sputum, wounds, and blood. a review of attack rates for acinetobacter was prompted by a dramatic increase in blood culture isolates. prospective surveillance of intensive care environment, ...198010315086
naturally occurring plasmids in acinetobacter calcoaceticus: pav2, a plasmid which influences the fertility of the sex factor pav1.acinetobacter calcoaceticus strain ebf65/65 harbours a cryptic plasmid, pav2, which has been shown by electrophoretic separation on agarose gels to have a molecular mass of approximately 13.5 megadaltons (md). transfer of the previously described sex factor pav1 (hinchliffe & vivian, 1980 a,b) from the hospital strainjc17 into strains possessing pav2 occurs only at a low frequency, whereas transfer to similar strains lacking pav2 occurs at a much higher frequency. in ebf65/65, pav1 may be presen ...19807264602
cell surface measurements in hydrocarbon and carbohydrate fermentations.acinetobacter calcoaceticus was grown in 11-liter batch fermentations with hexadecane or sodium citrate as the sole source of carbon. surface and interfacial tension measurements of the microbial broth indicated that surface-active compounds were being produced only during growth on the hydrocarbon substrate. contact angle measurements of an aqueous drop on a smooth lawn of cells in a hexadecane bath indicated a highly hydrophobic surface of the cells in the initial stages of the hydrocarbon fer ...198016345526
microbial flora of in-use, display eye shadow testers and bacterial challenges of unused eye shadows.we surveyed 15 different brands of eye shadow on display for customer use in different retail stores for microbial contamination. this was the first reported microbial surveillance of in-use eye shadow display testers in retail establishments. cultures were obtained at each retail store. sterile dacron swabs were rolled and rubbed over the entire used surface of each shadow, and each inoculum was streaked onto the surfaces of blood agar plates. of the 1,345 individual samples obtained, 67% were ...19817283427
role of adherence in growth of acinetobacter calcoaceticus rag-1 on hexadecane.the high affinity of acinetobacter calcoaceticus rag-1 for liquid hydrocarbons permitted the isolation of a spontaneous nonadherent mutant, mr-481. strain mr-481 exhibited no significant affinity for three test hydrocarbons, yet resembled the wild type in many properties, including production of the extracellular emulsifying agent emulsan. to study the role of adherence in growth on hydrocarbons, rag-1 and mr-481 were compared for growth on hexadecane under conditions of limited agitation and at ...19817287631
a cluster of acinetobacter pneumonia in foundry a 3-month period, three men who had worked for 5 to 19 years as welders or grinders of steel castings in a foundry acquired pneumonia caused by acinetobacter calcoaceticus variety anitratus serotype 7j. two of the men died, and postmortem examination showed mixed-dust pneumoconiosis with iron particles in the lungs. a calcoaceticus variety anitratus serotype 7j was isolated from the air in the foundry but the source was not found. the prevalence of antibody titers of 64 or greater to the 7j s ...19817305146
acinetobacter calcoaceticus-an opportunistic pathogen. 19817338827
isolation of acinetobacter calcoaceticus from an aborted equine foetus. 19817342938
meningitis caused by acinetobacter calcoaceticus var anitratus. a specific hazard in neurosurgical patients.acinetobacter calcoaceticus var anitratus caused meningitis in five patients between 1968 and 1978 at two hospitals affiliated with boston university school of medicine. all patients had had head trauma or neurosurgical procedures prior to the development of meningitis. the course of the disease was relatively indolent in that fulminant disease did not occur even when initial therapy was inappropriate and bacteria persisted in csf. all five patients survived. on gram's stain of csf, a calcoaceti ...19817469844
a comparison of the in vitro activity of cefoxitin and cephalothin against 7,312 clinical isolates.the antibacterial activity of cefoxitin and cephalothin was tested against 7,312 clinical isolates of aerobic pathogens. cefoxitin was found to have a broad spectrum of activity. among the gram-positive cocci, 99.1% of staphylococcus aureus, 94.4% of staphylococcus epidermidis and 98.1% of streptococci group b had a minimal inhibitory concentration of 16 microgram/ml or less, while only 4.6% of enterococci were sensitive to this concentration of cefoxitin. among the gram-negative rods 94.4% of e ...19817249786
adherence of acinetobacter calcoaceticus rag-1 to human epithelial cells and to hexadecane.the ability of acinetobacter calcoaceticus rag-1 to adhere to human epithelial cells was investigated and compared with its ability to adhere to a test hydrocarbon (hexadecane). rag-1, a microorganism originally isolated for growth on hydrocarbon, adhered to epithelial cells when grown under conditions which promote its adherence to hexadecane; similarly, rag-1 cells adhered poorly to epithelial cells when grown under conditions which cause the cells to possess low affinity towards hexadecane. a ...19817263064
aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans.garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ...19817240404
trimethoprim-sulfamethoxazole therapy for infective endocarditis.a man with stenosis of the aortic valve acquired endocarditis after abdominal surgery. klebsiella pneumoniae and acinetobacter calcoaceticus were cultured from his blood. the blood cultures remained positive despite intravenous gentamicin and cephalothin to which the organisms were sensitive in vitro. ultimately, the blood was sterilized by a combination of gentamicin and trimethoprim-sulfamethoxazole taken orally. the course of the patient was complicated by cardiac arrest and pericardial tampo ...19817029718
quinoprotein alcohol dehydrogenase from a non-methylotroph, acinetobacter calcoaceticus.acinetobacter calcoaceticus grown on ethanol contains an nad(p)+-independent alcohol dehydrogenase which resembles methanol dehydrogenase from methylotrophic bacteria in many respects. likewise, the prosthetic group of this enzyme appears to be identical to that of methanol dehydrogenase, namely, pyrrolo quinoline quinone. the organism is unable to grow on methanol, which means that quinoprotein alcohol dehydrogenases are not restricted to methylotrophs. arguments are presented for the idea that ...19817033448
microbial colonization of prosthetic devices. ii. scanning electron microscopy of naturally infected intravenous catheters.forty two nonselected naturally infected intravenous catheters were investigated by scanning electron microscopy (sem) and usual bacteriological methods. in many catheter samples an amorphous deposited substance could be detected by sem investigation, mostly associated with the isolation of staphylococci, acinetobacter calcoaceticus and pseudomonas aeruginosa. the thickest layers of such a substance were found in catheters infected by coagulase-negative staphylococci. the bacteria seemed to be c ...19816792814
bb-k 122: in vitro and in vivo activity against ocular 122 is a new aminoglycoside antibiotic and an analogue of amikacin. this study evaluated the in vitro and in vivo activity of bb-k 122 and gentamicin against important ocular pathogens. bb-k 122 and gentamicin demonstrated generally equivalent in vitro antibacterial activity, except that gentamicin was more active against streptococcus sp. bb-k 122 showed significant in vitro activity against important ophthalmic pathogens, including pseudomonas aeruginosa, staphylococcus aureus, acinetobac ...19816797791
evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-coa transferases from acinetobacter calcoaceticus and pseudomonas putida.homogeneous beta-ketoadipate succinyl-coa transferase (ec preparations were obtained from extracts of acinetobacter calcoaceticus and pseudomonas putida. gel filtration indicated that the respective transferases have similar molecular weights of 108,000 and 109,000; each transferase appears to have an alpha 2 beta 2 oligomeric structure formed by association of nonidentical subunits with a molecular weight of about 25,000. the subunits were separated by sodium dodecyl sulfate-gel electr ...19816780551
evolutionary relationships among gamma-carboxymuconolactone decarboxylases.gamma-carboxymuconolactone decarboxylase (ec from azotobacter vinelandii resembled the isofunctional enzymes from acinetobacter calcoaceticus and pseudomonas putida. all three decarboxylases appeared to be hexamers formed by association of identical subunits of about 13,300 daltons. the a. vinelandii and p. putida decarboxylases cross-reacted immunologically with each other, and the nh2-terminal amino acid sequences of the enzymes differed in no more than 7 of the first 36 residues. in ...19816783616
the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1.the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. in the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. the kinetics of antiserum-induced agglutination was quantified by measuring the time-dependent decrease in turbidity. for mutant isolation, a sample of the cells remaining in the supernata ...19816806434
properties of six pesticide degradation plasmids isolated from alcaligenes paradoxus and alcaligenes eutrophus.biophysical and genetic properties of six independently isolated plasmids encoding the degradation of the herbicides 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid are described. four of the plasmids, pjp3, pjp4, pjp5, and pjp7, had molecular masses of 51 megadaltons, belonged to the incp1 incompatibility group, and transferred freely to strains of escherichia coli, rhodopseudomonas sphaeroides, rhizobium sp., agrobacterium tumefaciens, pseudomonas putida, pseudomonas flu ...19816257648
in vitro antimicrobial activity of ceftizoxime against glucose-nonfermentative gram-negative rods.ceftizoxime, a new cephalosporin, was active against pseudomonas cepacia, flavobacterium meningosepticum, alcaligenes faecalis, and acinetobacter calcoaceticus and was more potent against pseudomonas aeruginosa and pseudomonas putida than was carbenicillin.19816269480
enzymatic adenylylation of spectinomycin by acinetobacter calcoaceticus subsp. anitratus.spectinomycin (spc) was inactivated in the presence of adenosine-5'-triphosphate and magnesium ion by an enzyme preparation made from acinetobacter calcoaceticus subsp. anitratus gn12313 resistant to both spc and streptomycin. the structure of the inactivated spc was found to be the adenylylated product of the hydroxy group on c-9 of the actinamine moiety.19816270047
acinetobacter spp.: distinct morphology on eosin methylene blue agar as an aid to identification in drinking water.acinetobacter calcoaceticus, frequently found in drinking waters and implicated in nosocomial infections, was presumptively identified by its tiny, blue colonial appearance on levine eosin methylene blue agar. all of the 33 isolates from drinking water showing this distinctive colonial appearance were identified as a. calcoaceticus.19816165324
acinetobacter calcoaceticus: some approaches to a problem. 19816174586
acinetobacter calcoaceticus causing subacute bacterial endocarditis. 19816114359
sensitivity of acinetobacter calcoaceticus strains to seven cephalosporins. 19816115226
[gamma-glutamyl-transferase activity in the family "neisseriaceae" (author's transl)].members of the family neisseriaceae (554 strains) were screened for gamma-glutamyl-transferase (gamma gt) activity. gamma gt was produced by neisseria meningitidis but not produced by any strain of n. gonorrhoeae, n. lactamica and branhamella catarrhalis. most non-proteolytic acinetobacter calcoaceticus produced gamma gt whereas most a. lwoffii failed to produce gamma gt.19826125120
phh502, a plasmid with incp and inci alpha characters, loses the latter by a specific reca-independent deletion event.plasmid phh502, of molecular weight 70 x 10(6), determined resistance to tetracycline, chloramphenicol, trimethoprim, sulphonamides and mercuric chloride and was incompatible with members of incp and inci alpha. it resembled other plasmids of inci alpha in the following properties: it determined pili that were morphologically and serologically i alpha pili, whose production was repressed in established plasmid-carrying (r+) cultures; its transfer was equally efficient in liquid or on solid mediu ...19826130120
transposon-mediated multiple antibiotic resistance in acinetobacter strains.acinetobacter calcoaceticus subsp. anitratus, which is unusually resistant to multiple antibiotics, was the cause of an epidemic of respiratory tract infections in patients in an intensive care unit. a representative isolate of the epidemic strain was found to contain the aminoglycoside-modifying enzymes 3-n-acetyltransferase, 3'-phosphotransferase, and 3"-adenylyltransferase, which confer resistance to gentamicin, kanamycin, and streptomycin, respectively. in addition, the strain produced a cep ...19826100428
[in vitro activity of ceftazidime compared with five beta-lactamase stable compounds against clinical strains of acinetobacter calcoaceticus ].acinetobacter calcoaceticus is recognized as one of the most resistant nosocomial pathogens. clinical isolates of acinetobacter are usually resistant to most beta-lactam antibiotics. the objective of this study was to evaluate the in vitro activity of ceftazidime, a new broad spectrum highly potent beta-lactam antibiotic, able to inhibit especially pseudomonas and providencia. its activity against 96 clinical strains of acinetobacter was compared with the activity of 5 recent beta-lactam antibio ...19826289227
ceftriaxone: in vitro studies and clinical evaluation.the in vitro activity of ceftriaxone against 437 clinical isolates of gram-negative bacilli was determined. ceftriaxone was found to have high in vitro activity against enterobacteriaceae, with the exception of enterobacter cloacae. ceftriaxone was only minimally active against pseudomonas aeruginosa and acinetobacter calcoaceticus. we evaluated the clinical efficacy and toxicity of ceftriaxone in 55 adult patients. bacterial infection was confirmed by the isolation of etiological bacteria in 30 ...19826289734
cefotaxime: a review of in vitro antimicrobial properties and spectrum of activity.cefotaxime has remarkable potency against all enterobacteriaceae, including enterobacter species, citrobacter freundii, serratia marcescens, and morganella morganii, proteus vulgaris, and providencia species--all of which are resistant to earlier cephalosporins. cefotaxime generally inhibits greater than 90% of enteric bacilli at concentrations of less than or equal to 0.5 microgram/ml; in one study it inhibited greater than 98% of isolates at less than or equal to 8 micrograms/ml. for staphyloc ...19826294779
comparative activity of cefotaxime and selected beta-lactam antibiotics against haemophilus influenzae and aerobic gram-negative bacilli.the activity of cefotaxime was compared with that of ampicillin, moxalactam, and cefoperozone against 50 isolates of haemophilus influenzae and with that of ampicillin, carbenicillin, cephalothin, cefoxitin, cefamandole, cefazolin, and several other established and investigational beta-lactam antibiotics against several hundred isolates of gram-negative aerobic enteric bacilli. minimal inhibitory concentrations of the drugs were determined by the agar plate dilution technique for h. influenzae a ...19826294790
[studies on acinetobacter calcoaceticus. the virulence and the therapeutic efficacy of antibiotics in mice]. 19826816860
[studies on acinetobacter calcoaceticus. protective effects of antiserum and vaccine to infection]. 19826816861
transfer of the gonococcal penicillinase plasmid: mobilization in escherichia coli by incp plasmids and isolation as a dna-protein relaxation complex.a 4.4-megadalton penicillinase plasmid, pwd2, from neisseria gonorrhoeae was transformed into escherichia coli. pwd2 was efficiently mobilized by incp plasmids in e. coli but not by flac, r1drd-19, or r64drd-11. pwd2 could be isolated as a dna-protein relaxation complex with properties similar to the well characterized cole1 complex. the host range of pwd2 was shown to include gonococci, enterobacteriaceae, and hemophilus influenzae, but not acinetobacter calcoaceticus or pseudomonas aeruginosa. ...19826801022
acinetobacter meningitis - a diagnostic pitfall. a report of 3 cases.three fatal cases of meningitis caused by acinetobacter calcoaceticus var. anitratus are reported, 1 in a premature infant who was being artificially ventilated and 2 in adults who had had neurosurgical operations. the microscopic resemblance of this organism to neisseria meningitidis is high-lighted and the problems of antimicrobial therapy are discussed.19826801786
[rubredoxin reductase in crude extracts of acinetobacter calcoaceticus in relation to carbon source and growth phase].by immunization of rabbits with discelectrophoretically purified rubredoxin-reductase from ac. calcoaceticus an antiserum against this enzyme was prepared. the antiserum was monospecifical to a diaphoretic activity, which had a rf-value identical with the rubredoxin-reductase-rf value. it has been shown by discelectrophoresis, immunoelectrophoresis, ouchterlony technique and kinetic investigations that crude extracts of bacteria grown on c16, malate, succinate or acetate contain rubredoxin-reduc ...19826803449
in vitro antibacterial activity of norfloxacin (mk-0366).the in vitro activity of norfloxacin (mk-0366) compared with that of beta-lactam antibiotics and, where appropriate of gentamicin or metronidazole was assessed against recent clinical isolates of common bacteria. the compound was highly active against most enterobacteria (minimal inhibitory concentrations [mics], 0.008 to 32 micrograms/ml; 90% inhibited by 0.25 micrograms/ml), haemophilus influenzae (mics, 0.03 to 0.12 micrograms/ml), and neisseria gonorrhoeae (mics, 0.008 to 0.016 micrograms/ml ...19826211139
evaluation of the automicrobic system for identification of glucose-nonfermenting gram-negative rods.with the enterobacteriaceae plus biochemical card, the automicrobic system (vitek systems, inc., hazelwood, mo.) is purported to identify members of the family enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: pseudomonas aeruginosa, p. fluorescens, p. putida, p. maltophilia, p. cepacia, and acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). the latter capability was examined in this investigation. of 410 glucose-nonfermenting rods included in the ...19827040451
isolation, purification, and chemical analysis of the lipopolysaccharide and lipid a of acinetobacter calcoaceticus nctc 10305.the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305 (london) was obtained by a modified phenol/chloroform/light petroleum method from the bacterial cells and from the culture medium in yields of 1.6% and 2.2% respectively (based on the bacterial dry weight). on chemical analysis, both preparations proved to be identical. the lipopolysaccharide obtained from the cells was purified by repeated ultracentrifugation, electrodialysis, and precipitation with sodium chloride. it was free of ...19827060573
different forms of quinoprotein aldose-(glucose-) dehydrogenase in acinetobacter calcoaceticus.the ratios of the oxidation rates of aldose sugars, determined in cell-free extracts of acinetobacter calcoaceticus, vary with the strain and growth conditions used. three distinct forms of glucose dehydrogenase with different substrate specificities, occurring in variable proportions in these extracts, are responsible for this effect. one form is the already known "soluble glucose dehydrogenase", the other two forms are complexes containing enzyme and components of the respiratory chain. the pr ...19827065812
weigle reactivation in acinetobacter calcoaceticus. 19827071193
[acinetobacter colcoaceticus: its properties, identification key and a study of its role in acute intestinal diseases].the authors present their new scheme for the identification of acinetobacter calcoaceticus, requiring a lesser number of test, and the characterstics of 84 strains isolated from patients with acute intestinal diseases and from the environment. some strains are atypical. acinetobacter has been detected in 7.8 +/- 1.0% of patients with acute intestinal diseases. the authors' observations confirm that the detection of acinetobacter in washings from such patients is more convincing for etiological d ...19827080744
the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods.we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ...19827087800
hand carriage of aerobic gram-negative rods by health care personnel.a quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic gram-negative rods by various groups of health care workers and 104 control subjects. overall, 31% of health care workers carried aerobic gram-negative rods on their hands compared to 59% of control subjects (p < 0.001). enterobacter agglomerans accounted for 40% of the isolates, and other enterobacter spp. 7%. other organisms included acinetobacter ...19827097001
acinetobacter meningitis: acquired infection in a neonatal intensive care unit.a cluster of 4 cases of meningitis due to acinetobacter calcoaceticus var anitratus occurred during a 5-day period in a neonatal intensive care unit. three of the infants were preterm and all had a history of other medical problems. initiation of intravenous therapy with carbenicillin was accompanied by clinical recovery and a bacteriological cure. intensive bacteriological investigation failed to show a common source for the infections.19827103551
nosocomial bacteremia due to acinetobacter 1980, 13 patients with positive blood cultures for acinetobacter calcoaceticus were detected in an 800 bed university medical center. twelve of the 13 isolates were identified as acinetobacter calcoaceticus var. anitratus and one as var. lwoffi. in the same period there were 361 positive specimens of a. calcoaceticus. eight of the patients were classified as having significant bacteremia (group a) with a serious infection in which acinetobacter was considered a significant pathogen. five addi ...19827107014
glucose-nonfermenting gram-negative bacilli associated with clinical veterinary specimens.glucose-nonfermenting gram-negative bacilli (nfb) have been recognized recently as opportunistic pathogens of humans. with few exceptions, strains of nfb have not been considered important enough to be identified when isolated from animals. in this study, all nfb isolated during a 1-year period in a clinical veterinary microbiology laboratory were identified to determine their prevalence. of the 347 strains of nfb obtained, the most common species were pseudomonas aeruginosa, acinetobacter calco ...19827107835
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