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biofilm formation and microbial activity in a biofilter system in the presence of mtbe, etbe and tame.emerging water contaminants derived from unleaded gasoline such as methyl tert-butyl ether (mtbe), ethyl tert-butyl ether (etbe) and tert-amyl methyl ether (tame), are in need of effective bioremediation technologies for restoring water resources. in order to design the conditions of a future groundwater bioremediating biofilter, this work assesses the potential use of acinetobacter calcoaceticus m10, rhodococcus ruber e10 and gordonia amicalis t3 for the removal of mtbe, etbe and tame in consor ...201121774959
a clpr-like regulator specifically recognizes a reca-independent promoter motif and broadly regulates expression of dna damage inducible genes in mycobacteria.the reca-dependent dna damage response pathway (sos response) appears to be the major dna repair mechanism in most bacteria, but it has been suggested that a reca-independent mechanism is responsible for controlling expression of most damage-inducible dna repair genes in mycobacterium tuberculosis. the specific reparative responses and molecular mediators involved in the dna repair mechanism remain largely unclear in this pathogen and its related species. in this study, a mycobacterial clpr-like ...201121771781
horizontal gene transfer/assortative recombination within the acinetobacter baumannii clinical population provides genetic diversity at the single caro gene encoding a major outer membrane protein channel.we described previously the presence in acinetobacter baumannii of a novel outer membrane (om) protein, caro, which functions as an l-ornithine om channel and whose loss was concomitant with increased carbapenem resistance among clonally related nosocomial isolates of this opportunistic pathogen. here, we describe the existence of extensive genetic diversity at the caro gene within the a. baumannii clinical population. the systematic analysis of caro sequences from a. baumannii isolates obtained ...201121764928
a comprehensive evaluation of colonic mucosal isolates of sutterella wadsworthensis from inflammatory bowel disease.inflammatory bowel disease (ibd) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. twenty-six clinical isolates of sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had ibd. s. wadsworthensis was initially more frequently isolated from ibd subjects, hence this comprehensive study was undertaken to elucidate its ro ...201122073125
biological improvement on combined mycelial pellet for aniline treatment by tourmaline in sbr process.as a biomass carrier, mycelial pellet of aspergillus niger y3 was used to immobilize the aniline-degrading bacterium acinetobacter calcoaceticus jh-9 and the mix culture of the cod rapid degrading bacteria in this study. tourmaline was added to this system in order to improve the aniline removal performance using combined mycelial pellet. flask experiments were performed to investigate the promotion mechanism. the results showed that the start-up time was shorted from 7 cycles to only 1 cycle. t ...201121764303
Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria.The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ...201122163022
identification and characterization of a new true lipase isolated through metagenomic approach.abstract: background: metagenomics, the application of molecular genomics to consortia of non-cultivated microbes, has the potential to have a substantial impact on the search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, ec 3.1.1.1) and lipases (triacylglycerol lipases, ec 3.1.1.3). in the current work, a novel lipase gene was identified from a fosmid metagenomic library constructed with the "prokaryotic-enriched" dna from a fat-contaminated soil collected from a wa ...201121762508
emergence of tigecycline & colistin resistant acinetobacter baumanii in patients with complicated urinary tract infections in north india. 201121727671
integrated analysis of three bacterial conjunctivitis trials of besifloxacin ophthalmic suspension, 0.6%: etiology of bacterial conjunctivitis and antibacterial susceptibility profile.the purpose of this paper is to report on the bacterial species isolated from patients with bacterial conjunctivitis participating in three clinical trials of besifloxacin ophthalmic suspension, 0.6%, and their in vitro antibacterial susceptibility profiles.201122034556
the effects of metabolite molecules produced by drinking water-isolated bacteria on their single and multispecies biofilms.the elucidation of the mechanisms by which diverse species survive and interact in drinking water (dw) biofilm communities may allow the identification of new biofilm control strategies. the purpose of the present study was to investigate the effects of metabolite molecules produced by bacteria isolated from dw on biofilm formation. six opportunistic bacteria, viz. acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staph ...201121732713
enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release.biosurfactants have been reported to utilize a number of immiscible substrates and thereby facilitate the biodegradation of panoply of polyaromatic hydrocarbons. olive oil is one such carbon source which has been explored by many researchers. however, studying the concomitant production of biosurfactant and esterase enzyme in the presence of olive oil in the bacillus species and its recombinants is a relatively novel approach.201121707984
renewable energy from cyanobacteria: energy production optimization by metabolic pathway engineering.the need to develop and improve sustainable energy resources is of eminent importance due to the finite nature of our fossil fuels. this review paper deals with a third generation renewable energy resource which does not compete with our food resources, cyanobacteria. we discuss the current state of the art in developing different types of bioenergy (ethanol, biodiesel, hydrogen, etc.) from cyanobacteria. the major important biochemical pathways in cyanobacteria are highlighted, and the possibil ...201121691792
whole-genome sequence of a multidrug resistant clinical isolate of acinetobacter lwoffii.acinetobacter lwoffii has been considered as opportunistic pathogen that can cause nosocomial infections in humans. here, we present the genome sequence of a clinical isolate, a. lwoffii wj10621, a multidrug resistant isolate that carries a plasmid with ndm-1 resistance gene.201121742884
Acinetobacter calcoaceticus- Acinetobacter baumannii complex species in clinical specimens in Singapore.SUMMARYThis study was performed to determine the prevalence, distribution of specimen sources, and antimicrobial susceptibility of the Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) species complex in Singapore. One hundred and ninety-three non-replicate Acb species complex clinical isolates were collected from six hospitals over a 1-month period in 2006. Of these, 152 (78-À7%) were identified as A. baumannii, 18 (9-À3%) as 'Acinetobacter pittii' [genomic species (gen. sp.) 3], and 23 ...201121733253
Impact of Carbapenem Resistance and Receipt of Active Antimicrobial Therapy on Clinical Outcomes of Acinetobacter baumannii Bloodstream Infections.Nosocomial Acinetobacter baumannii blood stream infections carry significant prevalence and mortality. The relationship between carbapenem resistance in A. baumannii and patient outcomes remains unclear. A retrospective cohort study was conducted on patients with A. baumannii bacteremia. Outcomes, controlling for confounders, were compared for carbapenem non-resistant A. baumannii (CNRAB) and carbapenem-resistant A. baumannii (CRAB). The primary outcome studied was all-cause hospital mortality, ...201121825287
decolorization characteristics and mechanism of victoria blue r removal by acinetobacter calcoaceticus yc210.acinetobacter calcoaceticus yc210 has been isolated and its ability to remove victoria blue r (vbr) from aqueous solution was assessed. the effects of various factors on decolorization efficiency were investigated in a batch system. the decolorization efficiency was found to be optimal within a ph of 5-7 and increased with vbr concentration up to 450 mg/l with high efficiency (94.5%) in a short time. the decolorization efficiency was significantly affected by cell concentrations. the decolorizat ...201121955656
identification of bacteria from the oral cavity and cloaca of snakes imported from vietnam.reptiles are used for various purposes these days, including public exhibits, medicinal applications, and as laboratory animals. as the international exchange of reptiles has gradually increased, more people have had the opportunity to come in contact with these animals. snakes typically live in the rhizosphere where various bacterial strains exist and as such they can lead to opportunistic human diseases. when snakes are encountered in veterinary medicine, it is necessary to monitor their micro ...201121998610
Different genospecies of Acinetobacter calcoaceticus-Acinetobacter baumannii complex associated with different outcomes. 201121967876
Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives.The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871, were investigated. Baeyer-Villiger oxygenation of 2-(2'-acetoxyethyl) cyclohexanone (MW 170 Da) into R-7-(2'-acetoxyethyl)-2-oxepanone was used as a model reaction. The whole-cell biotransformation followed Michaelis-Menten kinetics. The V (max) and K (S) va ...201121909677
diverse dna damage responses in acinetobacter include the capacity for dna damage-induced mutagenesis in the opportunistic pathogens acinetobacter baumannii and acinetobacter ursingii.error-prone and error-free dna damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics, or radiation sources were surveyed across the acinetobacter genus. the error prone sos mutagenesis response occurs when dna damage induces a cell's umudc- or dinp-encoded error-prone polymerases. the model acinetobacter baylyi strain adp1 possesses an unusual, regulatory umud allele (umudab) with an extended 5' region, and only incomplete fragments of umuc. d ...201122117008
recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing pseudomonas putida strain ncimb 10007.abstract: three different baeyer-villiger monooxygenases (bvmos) were reported to be involved in the camphor metabolism by pseudomonas putida ncimb 10007. during (+)-camphor degradation, 2,5-diketocamphane is formed serving as substrate for the 2,5-diketocamphane 1,2-monooxygenase. this enzyme is encoded on the cam plasmid and depends on the cofactors fmn and nadh and hence belongs to the group of type ii bvmos. we have cloned and recombinantly expressed the oxygenating subunit of the 2,5-diketo ...201121906366
comparative analysis of the complete genome of an acinetobacter calcoaceticus strain adapted to a phenol-polluted environment.the complete genome sequence of acinetobacter calcoaceticus phea-2, a non-pathogenic phenol-degrading bacterium previously isolated from industrial wastewater of an oil refinery in china, has been established. this is the first sequence of an a. calcoaceticus strain. we report here a comparative genomic analysis of phea-2 with two other acinetobacter species having different lifestyles, acinetobacter baumannii aye, a pathogenic human-adapted strain, and acinetobacter baylyi adp1, a soil-living s ...201122027104
Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925.ABSTRACT: Accidental oil spills and waste disposal are important sources for environmental pollution. We investigated the biodegradation of alkanes by Pseudomonas aeruginosa ATCC 55925 in relation to a rhamnolipid surfactant produced by the same bacterial strain. Results showed that the linear C11-C21 compounds in a heating oil sample degraded from 6% to 100%, whereas the iso-alkanes tended to be recalcitrant unless they were exposed to the biosurfactant; under such condition total biodegradatio ...201121906343
identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus.clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ...201122128805
multifunctional acyltransferases from tetrahymena thermophila.multifunctional acyltransferases are able to catalyze the esterification of various acyl-acceptors with activated fatty acids. here we describe the identification of four proteins from tetrahymena thermophila that share certain properties with mammalian acyltransferases regarding their predicted transmembrane structure, their molecular mass and the typical acyltransferase motif. expression of the tetrahymena sequences results in production of triacylglycerols and wax esters in recombinant yeast ...201122160552
enrichment and characterization of an autotrophic ammonia-oxidizing archaeon of mesophilic crenarchaeal group i.1a from an agricultural soil.soil nitrification is an important process for agricultural productivity and environmental pollution. though one cultivated representative of ammonia-oxidizing archaea from soil has been described, additional representatives warrant characterization. we describe an ammonia-oxidizing archaeon (strain my1) in a highly enriched culture derived from agricultural soil. fluorescence in situ hybridization microscopy showed that, after 2 years of enrichment, the culture was composed of >90% archaeal cel ...201122003023
diversity of 16s-23s rdna internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors.length polymorphisms within the 16s-23s ribosomal dna internal transcribed spacer (its) have been described as stable genetic markers for studying bacterial phylogenetics. in this study, we used these genetic markers to investigate phylogenetic relationships in burkholderia pseudomallei and its near-relative species. b. pseudomallei is known as one of the most genetically recombined bacterial species. in silico analysis of multiple b. pseudomallei genomes revealed approximately four homologous r ...201122195045
analysis of genes encoding penicillin-binding proteins in clinical isolates of acinetobacter baumannii.there is limited information on the role of penicillin-binding proteins (pbps) in the resistance of acinetobacter baumannii to β-lactams. this study presents an analysis of the allelic variations of pbp genes in a. baumannii isolates. twenty-six a. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in spain were included. the antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. based on the ...201121947403
multi-locus variable-number tandem repeat profiling of salmonella enterica serovar typhi isolates from blood cultures and gallbladder specimens from makassar, south-sulawesi, indonesia.multi-locus variable-number tandem repeat analysis differentiated 297 salmonella enterica serovar typhi blood culture isolates from makassar in 76 genotypes and a single unique s. typhi genotype was isolated from the cholecystectomy specimens of four patients with cholelithiasis. the high diversity in s. typhi genotypes circulating in makassar indicates that the number of carriers could be very large, which may complicate disease prevention and control.201121949819
Antimicrobial resistance determinants in imipenem-nonsusceptible Acinetobacter calcoaceticus-baumannii complex isolated in Daejeon, Korea.Members of the Acinetobacter calcoaceticus-baumannii (Acb) complex are important opportunistic bacterial pathogens and present significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we investigated the integrons and various genes involved in resistance to carbapenems, aminoglycosides, and fluoroquinolones in 56 imipenem-nonsusceptible Acb complex isolates.201122016680
Outbreak of imipenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii complex harboring different carbapenemase gene-associated genetic structures in an intensive care unit.BACKGROUND AND PURPOSE: To investigate the clinical and molecular epidemiology of the imipenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii (IRAcb) complex during an outbreak in an intensive care unit (ICU). METHODS: Forty-six clinical and 11 environmental isolates of the IRAcb complex were collected from the ICU of Taipei Veterans General Hospital, Taiwan between December 2003 and March 2004. These isolates were genotyped using pulsed-field gel electrophoresis (PFGE). Carbapene ...201122169123
rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish) from colony and blood culture material.multi-drug-resistant strains of the acinetobacter baumannii complex cause nosocomial infections. rapid identification of acinetobacter spp. is desirable in order to facilitate therapeutic or hygiene decisions. we evaluated a newly designed dna probe that can be used under standard conditions in both a microwave oven and a slide chamber for the rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish). using fish, the new probe correctly identified 81/81 acinetobacte ...201124516735
review of glucose oxidases and glucose dehydrogenases: a bird's eye view of glucose sensing enzymes.the evolution from first-generation through third-generation glucose sensors has witnessed the appearance of a number of very diverse oxidoreductases, which vary tremendously in terms of origin, structure, substrate specificity, cofactor used as primary electron acceptor, and acceptable final electron acceptor. this article summarizes our present knowledge of redox enzymes currently utilized in commercially available glucose monitoring systems to promote a fuller appreciation of enzymatic proper ...201122027299
distribution of genes encoding nucleoid-associated protein homologs in plasmids.bacterial nucleoid-associated proteins (naps) form nucleoprotein complexes and influence the expression of genes. recent studies have shown that some plasmids carry genes encoding nap homologs, which play important roles in transcriptional regulation networks between plasmids and host chromosomes. in this study, we determined the distributions of the well-known naps fis, h-ns, hu, ihf, and lrp and the newly found naps mvat and ndpa among the whole-sequenced 1382 plasmids found in gram-negative b ...201121350637
decolorization of textile dyes and degradation of mono-azo dye amaranth by acinetobacter calcoaceticus ncim 2890.acinetobacter calcoaceticus ncim 2890 (a. caloaceticus) was found to decolorize 20 different textile dyes of various classes. decolorization of an azo dye amaranth was observed effectively (91%) at static anoxic condition, whereas agitated culture grew well but showed less decolorization (68%) within 48 h of incubation. induction of intracellular and extracellular lignin peroxidase, intracellular laccase, dichlorophenol indophenol (dcip) reductase and riboflavin reductase represented their invol ...201123024414
phylogenetic study of sponge associated bacteria from the lakshadweep archipelago and the antimicrobial activities of their secondary metabolites.marine ecosystem of the lakshadweep archipelago is unique and known to have a very high degree of biodiversity with a number of endemic flora and fauna. the present study focuses to isolate the endosymbiotic microorganism from sponges and its effectiveness against marine ornamental fish pathogens. the sponges were collected from agatti island of lakshadweep archipelago and identified as clathria procera, sigmadocia fibulata and dysidea granulosa. in which, 15 different types of bacteria were iso ...201122806873
application of amplified ribosomal dna restriction analysis in identification of acinetobacter baumannii from a tertiary teaching hospital, malaysia.acinetobacter baumannii, genomic species 3 and 13tu are being increasingly reported as the most important acinetobacter species that cause infections in hospitalized patients. these acinetobacter species are grouped in the acinetobacter calcoaceticus- acinetobacter baumannii (acb) complex. differentiation of the species in the acb-complex is limited by phenotypic methods. therefore, in this study, amplified ribosomal dna restriction analysis (ardra) was applied to confirm the identity a. baumann ...201122433885
characterization and engineering of a novel pyrroloquinoline quinone dependent glucose dehydrogenase from sorangium cellulosum so ce56.a novel pyrroloquinoline quinone dependent glucose dehydrogenase like enzyme (pqq gdh) was isolated from sorangium cellulosum so ce56. the putative coding region was cloned, over expressed in e. coli and the resulting enzyme was characterized. the recombinant protein has a relative molecular mass of 63 kda and shows 43% homology to pqq gdh-b from acinetobacter calcoaceticus. in the presence of pqq and cacl₂ the enzyme has dehydrogenase activity with the substrate glucose as well as with other mo ...201120886312
rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis.we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ...201121289149
first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae.we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ...201121307209
first identification and characterization of an adeabc-like efflux pump in acinetobacter genomospecies 13tu.non-acinetobacter baumannii spp. are emerging among clinical acinetobacter isolates causing nosocomial infections, and some (such as genomospecies 13tu) appear to be multidrug resistant. the prevalence of non-acinetobacter baumannii spp. in the hospital setting is likely understated due to poor identification techniques. we report the first identification of an adeabc-type efflux pump in an acinetobacter genomospecies 13tu clinical isolate, its contribution to multidrug resistance, and the coexi ...201121199925
biofilm formation at the solid-liquid and air-liquid interfaces by acinetobacter species. 201121223561
biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, acinetobacter calcoaceticus tku024.two chitosanases (chsa1 and chsa2) were purified from the culture supernatant of acinetobacter calcoaceticus tku024 with squid pen as the sole carbon/nitrogen source. the molecular masses of chsa1 and chsa2 determined by sds-page were approximately 27 and 66 kda, respectively. the optimum ph, optimum temperature, ph stability, and thermal stability of chsa1 and chsa2 were (ph 6, 50°c, ph 4-10, <90°c) and (ph 7, 60°c, ph 6-11, <70°c), respectively. chsa1 and chsa2 had broad ph and thermal stabili ...201121225318
a shigella boydii bacteriophage which resembles salmonella phage vii.abstract: background: lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications. results: the lytic bacteriophage, phisbom-ag3, targets the important foodborne pathogen, shigella. it is morphologically similar to phage vii of salmonella enterica serovar typhi and a series of phages of acinetobacter calcoaceticus and rhizobium ...201121595934
genome sequence of acinetobacter calcoaceticus phea-2, isolated from industry wastewater.genome analysis of acinetobacter calcoaceticus phea-2 was undertaken because of the importance of this bacterium for bioremediation of phenol-polluted water and because of the close phylogenetic relationship of this species with the human pathogen acinetobacter baumannii. to our knowledge, this is the first strain of a. calcoaceticus whose genome has been sequenced.201121441526
characterization of n-acylhomoserine lactone-degrading bacteria associated with the zingiber officinale (ginger) rhizosphere: co-existence of quorum quenching and quorum sensing in acinetobacter and burkholderia.cell-to-cell communication (quorum sensing (qs)) co-ordinates bacterial behaviour at a population level. consequently the behaviour of a natural multi-species community is likely to depend at least in part on co-existing qs and quorum quenching (qq) activities. here we sought to discover novel n-acylhomoserine lactone (ahl)-dependent qs and qq strains by investigating a bacterial community associated with the rhizosphere of ginger (zingiber officinale) growing in the malaysian rainforest.201121385437
species identification and molecular characterization of acinetobacter spp. blood culture isolates from norway.the study investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates of acinetobacter species collected between 2005 and 2007 throughout norway.201121393175
cyclopropanation of membrane unsaturated fatty acids is not essential to the acid stress response of lactococcus lactis subsp. cremoris.cyclopropane fatty acids (cfas) are synthetized in situ by the transfer of a methylene group from s-adenosyl-l-methionine to a double bond of unsaturated fatty acid chains of membrane phospholipids. this conversion, catalyzed by the cfa synthase enzyme, occurs in many bacteria and is recognized to play a key role in the adaptation of bacteria in response to a drastic perturbation of the environment. the role of cfas in the acid tolerance response was investigated in the lactic acid bacterium lac ...201121421775
polymerase chain reaction assay for the detection of acinetobacter baumannii in endotracheal aspirates from patients in the intensive care unit.we aim to evaluate the efficacy of polymerase chain reaction (pcr) to detect acinetobacter baumannii in endotracheal aspirates.201121439512
novel regulator mphx represses activation of phenol hydroxylase genes caused by a xylr/dmpr-type regulator mphr in acinetobacter calcoaceticus.acinetobacter calcoaceticus phea-2 utilizes phenol as its sole carbon and energy source and has a multi-component phenol hydroxylase-encoding gene operon (mphklmnop) for phenol degradation. two additional genes, mphr and mphx, were found upstream and downstream of mphklmnop, respectively. the mphr gene encodes a xylr/dmpr-type regulator-like protein and is transcribed in the opposite direction to mphklmnop. the mphx gene is transcribed in the same direction as mphklmnop and encodes a protein wit ...201121455294
microbiology of animal bite wound infections.the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ...201121482724
antibacterial activity in spices and local medicinal plants against clinical isolates of karachi, pakistan.context: development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. twenty-seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study. eleven plants chosen from these 27 are used as spices in local cuisine. objective: evaluation of the effectiveness of some medicinal plant extracts a ...201121501041
Comparative genomics of the type VI secretion systems of Pantoea and Erwinia species reveals the presence of putative effector islands that may be translocated by the VgrG and Hcp proteins.ABSTRACT:201122115407
synergistic effect of thermophilic temperature and biosurfactant produced by acinetobacter calcoaceticus bu03 on the biodegradation of phenanthrene in bioslurry system.this study aimed at investigating the synergistic effect of temperature and biosurfactant on the biodegradation of phenanthrene in bioslurry. bench-scale bioslurry experiments were conducted at 25 and 55°c. the desorption rate coefficients of phenanthrene (k(des)) obtained using the pseudo-first order model were 0.0026 and 0.0035kgmg(-1)h(-1) at 25 and 55°c, respectively. addition of 1500mgl(-1) biosurfactant, produced by acinetobacter calcoaceticus bu03, marginally increased the k(des) at 25°c ...201121530078
biofilm formation and adherence characteristics of an elizabethkingia meningoseptica isolate from oreochromis mossambicus.abstract: background: elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. their ability to exist as biofilm structures has been alluded to but not extensively investigated. methods: the ability of elizabethkingia meningoseptica isolate ch2b from freshwater tilapia (oreochromis mossambicus) and e. meningoseptica strain nctc 10016t to adhere to abiotic surfaces was investigated using microtiter ...201121545730
infection in conflict wounded.although mechanisms of modern military wounding may be distinct from those of ancient conflicts, the infectious sequelae of ballistic trauma and the evolving microbial flora of war wounds remain a considerable burden on both the injured combatant and their deployed medical systems. battlefield surgeons of ancient times favoured suppuration in war wounding and as such galenic encouragement of pus formation would hinder progress in wound care for centuries. napoleonic surgeons eventually abandoned ...201121149356
the acinetobacter baumannii reca protein in the repair of dna damage, antimicrobial resistance, general stress response, and virulence.reca is the major enzyme involved in homologous recombination and plays a central role in sos mutagenesis. in acinetobacter spp., including a. baumannii, a multidrug-resistant bacterium responsible for nosocomial infections worldwide, dna repair responses differ in many ways from those of other bacterial species. in this work, the function of a. baumannii reca was examined by constructing a reca mutant. alteration of this single gene had a pleiotropic effect, showing the involvement of reca in d ...201121642465
amylolytic microorganism from s+úo paulo zoo composting: isolation, identification, and amylase production.composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. this transformation is the result of the microorganism action, converting complex carbon sources into energy. enzymes that are exported by the microorganisms to the surrounding environment mediate this process. the aiming of the present work is to prospect the compost produced by the organic composting unit (ocu) of the funda+º+úo parque zool+¦gic ...201121845217
genotypic and phenotypic characterization of the acinetobacter calcoaceticus-acinetobacter baumannii complex with the proposal of acinetobacter pittii sp. nov. (formerly acinetobacter genomic species 3) and acinetobacter nosocomialis sp. nov. (formerly acinetobacter genomic species 13tu).acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13tu are increasingly recognized as clinically important taxa within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. to define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the acb complex. all strains were characterized by aflp analysis, amplified rdna restriction analysis and nutritional or physiological testing, while selected strains were studied by ...201121320596
advances in the management of sepsis and the understanding of key immunologic defects.anesthesiologists are increasingly confronting the difficult problem of caring for patients with sepsis in the operating room and in the intensive care unit. sepsis occurs in more than 750,000 patients in the united states annually and is responsible for more than 210,000 deaths. approximately 40% of all intensive care unit patients have sepsis on admission to the intensive care unit or experience sepsis during their stay in the intensive care unit. there have been significant advances in the un ...201121952252
the role of synthetic biology in the design of microbial cell factories for biofuel production.insecurity in the supply of fossil fuels, volatile fuel prices, and major concerns regarding climate change have sparked renewed interest in the production of fuels from renewable resources. because of this, the use of biodiesel has grown dramatically during the last few years and is expected to increase even further in the future. biodiesel production through the use of microbial systems has marked a turning point in the field of biofuels since it is emerging as an attractive alternative to con ...201122028591
infection-associated clinical outcomes in hospitalized medical evacuees after traumatic injury: trauma infectious disease outcome study. 201121795875
use of growing cells of pseudomonas aeruginosa for synthesis of the natural vanillin via conversion of isoeugenol.the great demand of people for consumption of natural additives resulted in producing natural vanillin. there are plant sources and chemical procedures for vanillin production but microbial bioconversions are being sought as a suitable alternative. in the present work, the ability to produce vanillin from isoeugenol was screened using growing cultures of various bacteria. among the 56 strains of bacteria isolated from the soil environments of iran, a gram-negative rod designated as strain ispc2 ...201124250410
functional characterization of a ficolin-mediated complement pathway in amphioxus.the ficolin-mediated complement pathway plays an important role in vertebrate immunity, but it is not clear whether this pathway exists in invertebrates. here we identified homologs of ficolin pathway components from the cephalochordate amphioxus and investigated whether they had been co-opted into a functional ficolin pathway. four of these homologs, ficolin fcn1, serine protease masp1 and masp3, and complement component c3, were highly expressed in mucosal tissues and gonads, and were signific ...201121832079
evolutionary view of acyl-coa diacylglycerol acyltransferase (dgat), a key enzyme in neutral lipid biosynthesis.triacylglycerides (tags) are a class of neutral lipids that represent the most important storage form of energy for eukaryotic cells. dgat (acyl-coa: diacylglycerol acyltransferase; ec 2.3.1.20) is a transmembrane enzyme that acts in the final and committed step of tag synthesis, and it has been proposed to be the rate-limiting enzyme in plant storage lipid accumulation. in fact, two different enzymes identified in several eukaryotic species, dgat1 and dgat2, are the main enzymes responsible for ...201121933415
enterobacteriaceae in mouth and cloaca of podocnemis expansa and p. unifilis (testudines: chelonia) populations of national park of araguaia plains, brazil.shigella flexnerii and escherichia coli were the most frequent gram-negative bacteria found in the mouth cavity and cloacae of the turtles podocnemis expansa and p. unifilis on beaches in the national park of araguaia, brazil. reptiles are known as salmonella carriers, despite rarely isolated in these turtles.201124031664
bacterial identification by 16s rrna gene pcr-hybridization as a supplement to negative culture results.pcr-hybridization was compared to culture methods for evaluating suspected blood infections. a total of 231 clinical samples from blood culture bottles that were flagged positive by the bact/alert system or were negative 1 week after inoculation were tested. when the pcr-hybridization and culture method results were compared, the positive and negative concordance rates were 99.2% (122/123) and 89.5% (94/105), respectively. of the negative blood cultures, 10.5% (11/105) were positive by pcr-hybri ...201121430102
environmental applications of biosurfactants: recent advances.increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. an alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. the diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide varie ...201121340005
occurrence of plasmids in the aromatic degrading bacterioplankton of the baltic sea.plasmids are mobile genetic elements that provide their hosts with many beneficial traits including in some cases the ability to degrade different aromatic compounds. to fulfill the knowledge gap regarding catabolic plasmids of the baltic sea water, a total of 209 biodegrading bacterial strains were isolated and screened for the presence of these mobile genetic elements. we found that both large and small plasmids are common in the cultivable baltic sea bacterioplankton and are particularly prev ...201124710296
hexavalent chromium reduction and accumulation by acinetobacter ab1 isolated from fez tanneries in morocco.hexavalent chromium reduction and accumulation by acinetobacter ab1 isolated from fez tanneries effluents were tested. the effects of some environmental factors such as ph, temperature, and exposure time on cr(vi) reduction and resistance were investigated. we found that this strain was able to resist to concentrations as high as 400 mg/l of cr(vi). moreover, ph 10 and the temperature 30°c constitute favourable conditions to the growth and reduction of acinetobacter ab1. complete reduction of cr ...201123459068
ecological significance of microdiversity: coexistence among casing soil bacterial strains through allocation of nutritional resource.a combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16s rrna gene sequences can represent distinct eco- and genotypes. a set of eight bacterial strains wherein three were pseudomonas putida and rest were acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. these strains had identical 16s rrna gene sequences and represented the dominant phylotype in the plateable frac ...201122282622
pharmacokinetics and safety of single and multiple doses of achn-490 injection administered intravenously in healthy subjects.achn-490 is an aminoglycoside with activity against multidrug-resistant pathogens, including those resistant to currently used aminoglycosides. two randomized, double-blind, placebo-controlled clinical studies investigated the pharmacokinetics (pk), safety, and tolerability of achn-490 injection in healthy subjects. study 1 used a parallel-group design with escalating single (sd) and multiple doses (md). study 2 explored a longer duration of the highest dose tolerated in the first study. subject ...201121911572
comparison of genospecies and antimicrobial resistance profiles of isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from various clinical specimens.this study was conducted to compare the prevalences of antimicrobial resistance profiles of clinical isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from sterile and nonsterile sites and to further study the relationship of antimicrobial resistance profiles and genospecies by amplified rrna gene restriction analysis (ardra). a total of 1,381 isolates were tested with 12 different antibiotics to show their antimicrobial susceptibility profiles. a total of 205 clinical ...201223006758
recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii complex.this study investigated the clinical and microbiological characteristics of patients with recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex at a medical center. all acb complex isolates associated with recurrent bacteremia were identified to the genomic species level using a 16s-23s rrna gene intergenic spacer sequence-based method. genotypes were determined by the random amplified polymorphic dna patterns generated by arbitrarily primed pcr and ...201222760035
expression of the resistance-nodulation-cell division pump adeijk in acinetobacter baumannii is regulated by aden, a tetr-type regulator.resistance-nodulation-division efflux system adeijk contributes to intrinsic resistance to various drug classes in acinetobacter baumannii. by whole-genome sequencing, we have identified in clinical isolate bm4587 the aden gene, located 813 kbp upstream from adeijk, which encodes a tetr transcriptional regulator. in one-step mutant bm4666 overexpressing adeijk, the deletion of cytosine 582 (c(582)) in the 3' portion of this gene was responsible for a frameshift mutation resulting in the deletion ...201222371895
screening and characterization of a novel alkaline lipase from acinetobacter calcoaceticus 1-7 isolated from bohai bay in china for detergent formulation.a novel alkaline lipase-producing strain 1-7 identified as acinetobacter calcoaceticus was isolated from soil samples collected from bohai bay, china, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. the lipase from strain 1-7 showed the maximum activity at ph 9.0 under 40 °c. one interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. it is also stable at a broad range of phs from 4.0 to ...201224031813
identification of acinetobacter species: is bruker biotyper maldi-tof mass spectrometry a good alternative to molecular techniques?acinetobacter spp. has become a leading cause of nosocomial infection in recent years. phenotypic similarities between the species in the genus have made it difficult to identify them clearly using routine diagnostic methods. consequently, more relevant species have been grouped together as acinetobacter calcoaceticus-acinetobacter baumannii complex (a. baumannii, a. calcoaceticus, acinetobacter genospecies 3 and a. genospecies 13tu). however, there are other species that may also have clinical ...201222266021
isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating pvc materials.waste materials containing di-(2-ethylhexyl) phthalate (dehp), a suspected endocrine disruptor and reasonably anticipated human carcinogen, are typically disposed of in landfills. despite this, very few studies had been conducted to isolate and identify dehp-degrading bacteria in landfill leachate. therefore, this study was conducted to isolate and characterize bacteria in landfill leachate growing on dehp as the sole carbon source and deteriorating pvc materials. four strains lhm1, lhm2, lhm3 a ...201222934997
reductive dechlorination of methoxychlor by bacterial species of environmental origin: evidence for primary biodegradation of methoxychlor in submerged environments.methoxychlor [1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane] is an organochlorine insecticide that undergoes dechlorination in natural submerged environments. we investigated the ability to dechlorinate this compound in seven environmental bacterial species ( aeromonas hydrophila , enterobacter amnigenus , klebsiella terrigena , bacillus subtilis , achromobacter xylosoxidans , acinetobacter calcoaceticus , and mycobacterium obuense ) and the enteric bacterium escherichia coli as a positive cont ...201222292429
cellular fatty acids as chemical markers for differentiation of acinetobacter baumannii and acinetobacter calcoaceticus.gas chromatography (gc) was used to investigate the cellular fatty acid (cfa) composition of 141 acinetobacter baumannii and 32 a. calcoaceticus isolates from different locations in china and to find chemical markers to differentiate these two closely related bacteria.201223228842
comparative analysis of fecal microflora of healthy full-term indian infants born with different methods of delivery (vaginal vs cesarean): acinetobacter sp. prevalence in vaginally born infants.in this study fecal microflora of human infants born through vaginal delivery (vb) and through cesarean section (cb) were investigated using culture-independent 16s rdna cloning and sequencing approach. the results obtained clearly revealed that fecal microbiota of vb infants distinctly differ from those in their counterpart cb infants. the intestinal microbiota of infants delivered by cesarean section appears to be more diverse, in terms of bacteria species, than the microbiota of vaginally del ...201223151789
identification of genetic recombination between acinetobacter species based on multilocus sequence analysis.during multilocus sequence analysis of acinetobacter calcoaceticus-acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 acinetobacter species. while 3 isolates belonged to a. nosocomialis based on 16s rrna, gyrb, fusa, gdhb, and rplb gene sequences, they showed close relationships with acinetobacter genomic species 'close to 13tu' in rpob, reca, cpn60, rpod, and glta gene trees.201222595610
clinical characteristics of tertiary hospital patients from whom acinetobacter calcoaceticus-acinetobacter baumannii complex strains were isolated.acinetobacter baumannii is a worldwide nosocomial pathogen that has become increasingly common over the past few decades, and strains of multidrug-resistant a. baumannii have been increasing. the aim of this study was to assess the clinical characteristics of a. calcoaceticus-a. baumannii complex (acb complex) strains and to determine the risk factors of this infection.201222214623
characterization of rhamnolipids produced by non-pathogenic acinetobacter and enterobacter bacteria.rhamnolipid production by two non-pathogenic bacterial strains acinetobacter calcoaceticus and enterobacter asburiae, and established rhamnolipid producer pseudomonas aeruginosa was investigated. rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homolo ...201223313768
acinetobacter calcoaceticus-baumannii complex strains induce caspase-dependent and caspase-independent death of human epithelial cells.we investigated interactions of human isolates of acinetobacter calcoaceticus-baumannii complex strains with epithelial cells. the results showed that bacterial contact with the cells as well as adhesion and invasion were required for induction of cytotoxicity. the infected cells revealed hallmarks of apoptosis characterized by cell shrinking, condensed chromatin, and internucleosomal fragmentation of nuclear dna. the highest apoptotic index was observed for 4 of 10 a. calcoaceticus and 4 of 7 a ...201222684803
Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea.Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ...201222130193
changes in antimicrobial susceptibility and major clones of acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in korea over 7 years.acinetobacter species have emerged as opportunistic nosocomial pathogens in intensive care units. epidemic spread and outbreaks of multidrug-resistant or carbapenem-resistant acinetobacter baumannii infections have been described worldwide. species distribution, antimicrobial resistance and genotypes were investigated for acinetobacter species isolates collected from a single institution in korea over 7 years. two hundred and eighty-seven acinetobacter species isolates were collected from patien ...201221852525
vertical immobilization of viable bacilliform bacteria into polypyrrole films.we have successfully developed a novel technique for inserting viable bacilliform bacteria into polypyrrole films. all of the five different bacterial cells (pseudomonas aeruginosa, acinetobacter calcoaceticus, serratia marcescens, bacillus subtilis and escherichia coli) studied in this work were inserted normal to the film surface, and the viability of p. aeruginosa was unaffected by this immobilization procedure. it was also found that the polypyrrole layer was important to keep the cells aliv ...201222498456
multitrophic interaction in the rhizosphere of maize: root feeding of western corn rootworm larvae alters the microbial community composition.larvae of the western corn rootworm (wcr) feeding on maize roots cause heavy economical losses in the us and in europe. new or adapted pest management strategies urgently require a better understanding of the multitrophic interaction in the rhizosphere. this study aimed to investigate the effect of wcr root feeding on the microbial communities colonizing the maize rhizosphere.201222629377
a five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by acinetobacter radioresistens sh164.acinetobacter calcoaceticus sw1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (tauxy). blast searches of newly available genome sequences using the tauxy sequences revealed a 5-gene cluster, taurxypi, in acinetobacter radioresistens sh164. we hypothesized that tauxypi (hmpref0018_00717-hmpref0018_00720) encodes proteins that are orthologs of the undefined pathway from st ...201222588221
domiciliary cockroaches found in restaurants in five zones of kuala lumpur federal territory, peninsular malaysia.the following domiciliary cockroaches were collected from restaurants in five zones of kuala lumpur federal territory, malaysia using 1l glass beaker traps baited with ground mouse-pellets: periplaneta americana (linnaeus) (n = 820), periplaneta brunnea burmeister (n = 46), blattella germanica (linnaeus) (n = 12504), supella longipalpa (fabricius) (n = 321), symploce pallens stephens (n = 29) and neostylopyga rhombifolia (stoll) (n = 5). the following bacteria were isolated from 10 cockroach spe ...201222543619
infections due to different genospecies of the acinetobacter calcoaceticus-a baumannii complex. 201222526214
[synthesis of surfactants acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5070 in the medium with glycerol].it was established that glycerol, a byproduct of biodiesel production, may be used as substrate for synthesis of surfactants rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241. maximum indices of surfactants synthesis by the strain imv b-7241 have been fixed, when the medium with glycerol included yeast autolysate and trace elements. it was shown that the surfactants synthesis could be intensified when cultivating a. calcoaceticus imv b-7241 and r. erythropolis imv a ...201322545440
inoculation of phosphate solubilizing bacteria for the improvement of lead accumulation by brassica juncea.two phosphate-solubilizing bacterial strains were isolated and identified as acinetobacter calcoaceticus yc-5a and enterobacter agglomerans kmc-7 based on the 16s rrna gene sequence analysis. a. calcoaceticus yc-5a is less well known as a phosphate-solubilizing plant-associated bacterium. the plant growth-promoting properties of the phosphate-solubilizing bacteria (psb) were characterized in vitro, including their phosphate-solubilizing activities and their capabilities for producing indole-3-ac ...201323530360
antimicrobial susceptibility pattern in nosocomial infections caused by acinetobacter species in asir region, saudi arabia.this study aimed at evaluating the sensitivity of antibiotics towards nosocomial infections caused by acinetobacter species. the study took place during the period dec. 2011- dec. 2012 at assir central hospital in collaboration with the department of microbiology, college of medicine, king khalid university, abha. a prospective study involving 150 patients presented with nosocomial infections due to acinetobacter species detected by bacteriological tests; direct microscopy, culture in blood agar ...201324498790
emerging threat of multidrug resistant bugs--acinetobacter calcoaceticus baumannii complex and methicillin resistant staphylococcus aureus.infections caused by bacteria such as multidrug resistant (mdr) acinetobacter spp. and methicillin-resistant staphylococcus aureus (mrsa) constitute a worldwide pandemic. without gathering information about these strains, we cannot reduce the morbidity and mortality due to infections caused by these notorious bugs.201323497675
repeated colonization by multi-drug-resistant acinetobacter calcoaceticus-a. baumannii complex and changes in antimicrobial susceptibilities in surgical intensive care units.a nosocomial outbreak of multi-drug-resistant acinetobacter calcoaceticus-a. baumannii (mdr-acb) complex infection occurred in a newly constructed building at a 2,500-bed tertiary medical center in taiwan.201323425282
antibacterial and antiplasmodial constituents of beilschmiedia cryptocaryoides.four new beilschmiedic acid derivatives, cryptobeilic acids a-d (1-4), and tsangibeilin b (5) have been isolated from the bark of beilschmiedia cryptocaryoides collected from madagascar. their structures were elucidated using detailed spectroscopic and spectrometric methods. cryptobeilic acid a (1) and tsangibeilin b (5) structures were confirmed by single-crystal x-ray diffraction analysis. compounds 1-5 displayed moderate antibacterial activity against escherichia coli 6r3, acinetobacter calco ...201323320609
[effects of oil-refining microbes (genus acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].the objective of this study was to assess ability of oil-refining bacteria acinetobacter calcoaceticus and a. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. it was found that the cultures infected with a. calcoaceticus showed significantly high frequencies of cytogenetical effects and chromosomal aberrant cells as compared to the intact cultures and cultures infected with a. valentis. the most of chromosomal aberrations, mainly chroma ...201323285752
[intensification of biosurfactant synthesis by acinetobacter calcoaceticus imv b-7241 on a hexadecane–glycerol mixture ]. 201323234072
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