Publications

improved laboratory enrichment for enterohemorrhagic escherichia coli by exposure to extremely acidic conditions.	analysis of food samples for e. coli o157:h7 using the standard u.s. food and drug administration procedure is frequently complicated by overgrowth of nontarget microorganisms. a new procedure was developed for enrichment of enterohemorrhagic e. coli (ehec) which utilizes exposure to ph 2.00 for 2 h. this procedure yielded larger populations of ehec than the standard method by factors ranging from 2.7 to 7.7 and, when age-stressed cultures were used, by factors ranging from 2.7 to 11.5. cultures ...	2004	14766610
biased distribution of dna uptake sequences towards genome maintenance genes.	repeated sequence signatures are characteristic features of all genomic dna. we have made a rigorous search for repeat genomic sequences in the human pathogens neisseria meningitidis, neisseria gonorrhoeae and haemophilus influenzae and found that by far the most frequent 9-10mers residing within coding regions are the dna uptake sequences (dus) required for natural genetic transformation. more importantly, we found a significantly higher density of dus within genes involved in dna repair, recom ...	2004	14960717
[effect of delay of blood cultures on positive detection by automated blood culture system].	the effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; bactec 9240 system and bact/alert 3d system. total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. bactec 92f, 93f and bact/alert fa, fn bottles were used as the blood culture bottles. all the bottles inoc ...	2004	15628528
occurrence and characterization of mercury resistance in the hyperthermophilic archaeon sulfolobus solfataricus by use of gene disruption.	mercury resistance mediated by mercuric reductase (mera) is widespread among bacteria and operates under the control of merr. merr represents a unique class of transcription factors that exert both positive and negative regulation on gene expression. archaea and bacteria are prokaryotes, yet little is known about the biological role of mercury in archaea or whether a resistance mechanism occurs in these organisms. the archaeon sulfolobus solfataricus was sensitive to mercuric chloride, and low-l ...	2004	14702312
comparison in a rat thigh abscess model of imipenem, meropenem and cefoperazone-sulbactam against acinetobacter baumannii strains in terms of bactericidal efficacy and resistance selection.	we compared imipenem, meropenem and cefoperazone-sulbactam against hospital originated a. baumannii strains in terms of bactericidal efficacy and selection of resistant mutants during treatment in a rat thigh abscess model.	2004	14713318
pqq glucose dehydrogenase with novel electron transfer ability.	pqq glucose dehydrogenase from acinetobacter calcoaceticus (gdh-b) is one of the most industrially attractive enzymes, as a sensor constituent for glucose sensing, because of its high catalytic activity and insensitivity to oxygen. we attempted to engineer gdh-b to enable electron transfer to the electrode in the absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase (qh-edh) from comamonas testosteroni, which is composed of a pqq- ...	2004	14741705
genetic environment and transcription of ampc in an acinetobacter baumannii clinical isolate.	an ampc gene was cloned from a clinical isolate of acinetobacter baumannii (strain ran). dna sequencing and primer extension studies showed that ampc is transcribed from a promoter contained within a putative insertion sequence element which has been found to abut several different genes in acinetobacter spp.	2004	14742218
response of biofilm bacteria to dissolved organic matter from decomposing maple leaves.	stream bacteria play an important role in the utilization of dissolved organic matter (dom) leached from leaves, and in transfer of this dom to other trophic levels. leaf leachate is a mixture of labile, recalcitrant, and inhibitory compounds, and bacterial communities vary in their ability to utilize leachate. the purpose of this study was to determine the effects of dom from sugar maple leaves on bacterial populations in biofilms on decomposing leaf surfaces. populations of acinetobacter calco ...	2004	15692852
biosorption and recycling of gold using various microorganisms.	in order to obtain basic information on the biosorption and recycling of gold from aqueous systems using microbial cells, the biosorption of gold by various microorganisms was investigated. of 75 strains of microorganisms tested (25 bacteria, 19 actinomycetes, 17 fungi and 14 yeasts), high abilities of gold biosorption from a solution containing hydrogen tetrachloroaurate (iii) were found in some gram-negative bacterial strains, such as acinetobacter calcoaceticus, erwinia herbicola, pseudomonas ...	2004	15754248
fluoroquinolone resistance from a transferable plasmid in acinetobacter calcoaceticus.		2004	16295408
the trna world.		2004	14970379
determination of enzyme mechanisms by molecular dynamics: studies on quinoproteins, methanol dehydrogenase, and soluble glucose dehydrogenase.	molecular dynamics (md) simulations have been carried out to study the enzymatic mechanisms of quinoproteins, methanol dehydrogenase (mdh), and soluble glucose dehydrogenase (sgdh). the mechanisms of reduction of the orthoquinone cofactor (pqq) of mdh and sgdh involve concerted base-catalyzed proton abstraction from the hydroxyl moiety of methanol or from the 1-hydroxyl of glucose, and hydride equivalent transfer from the substrate to the quinone carbonyl carbon c5 of pqq. the products of methan ...	2004	15273299
microscale and molecular assessment of impacts of nickel, nutrients, and oxygen level on structure and function of river biofilm communities.	studies were carried out to assess the influence of nutrients, dissolved oxygen (do) concentration, and nickel (ni) on river biofilm development, structure, function, and community composition. biofilms were cultivated in rotating annular reactors with river water at a do concentration of 0.5 or 7.5 mg liter(-1), with or without a combination of carbon, nitrogen, and phosphorus (cnp) and with or without ni at 0.5 mg liter(-1). the effects of ni were apparent in the elimination of cyanobacterial ...	2004	15240316
improved method for polynucleotide probe-based cell sorting, using dna-coated microplates.	we developed an improved method for cultivation-independent sorting of bacterial cells. the technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the cells. target cells are immobilized during a second hybridization step in microplates that are coated with dna that is complementary to the probe sequence. the method was applied suc ...	2004	14711680
ventilator associated pneumonia: comparison between quantitative and qualitative cultures of tracheal aspirates.	deferred or inappropriate antibiotic treatment in ventilator-associated pneumonia (vap) is associated with increased mortality, and clinical and radiological criteria are frequently employed to establish an early diagnosis. culture results are used to confirm the clinical diagnosis and to adjust or sometimes withdraw antibiotic treatment. tracheal aspirates have been shown to be useful for these purposes. nonetheless, little is known about the usefulness of quantitative findings in tracheal secr ...	2004	15566587
computer-assisted surveillance for detecting clonal outbreaks of nosocomial infection.	whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. we developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital-wide basis. monthly total isolates of 25 clinically significant hospital pathogens were tallied from 1991 to 1998 to form a database for future comparison. two different algorithm ...	2004	15004070
neutral lipid bodies in prokaryotes: recent insights into structure, formation, and relationship to eukaryotic lipid depots.		2005	15901682
bench-to-bedside review: antimicrobial utilization strategies aimed at preventing the emergence of bacterial resistance in the intensive care unit.	antimicrobial resistance has emerged as one of the most important issues complicating the management of critically ill patients with infection. this is largely due to the increasing presence of pathogenic microorganisms with resistance to existing antimicrobial agents resulting in the administration of inappropriate treatment. effective strategies for the prevention of antimicrobial resistance within intensive care units are available and should be aggressively implemented. the importance of pre ...	2005	16277734
seasonal response of stream biofilm communities to dissolved organic matter and nutrient enrichments.	dissolved organic matter (dom) and inorganic nutrients may affect microbial communities in streams, but little is known about the impact of these factors on specific taxa within bacterial assemblages in biofilms. in this study, nutrient diffusing artificial substrates were used to examine bacterial responses to dom (i.e., glucose, leaf leachate, and algal exudates) and inorganic nutrients (nitrate and phosphate singly and in combination). artificial substrates were deployed for five seasons, fro ...	2005	15870312
incidence of class 1 integrons in a quaternary ammonium compound-polluted environment.	samples of effluent and soil were collected from a reed bed system used to remediate liquid waste from a wool finishing mill with a high use of quaternary ammonium compounds (qacs) and were compared with samples of agricultural soils. resistance quotients of aerobic gram-negative and gram-positive bacteria to ditallowdimethylammomium chloride (dtdmac) and cetyltrimethylammonium bromide (ctab) were established by plating onto nutrient agar containing 5 microg/ml or 50 microg/ml dtdmac or ctab. ap ...	2005	15855499
novel major bacterial candidate division within a municipal anaerobic sludge digester.	in a previous study, we analyzed the molecular diversity of planctomycetales by pcr amplification and sequencing of 16s rrna clone libraries generated from a municipal wastewater plant, using planctomycete-specific and universal primer sets (r. chouari, d. le paslier, p. daegelen, p. ginestet, j. weissenbach, and a. sghir, appl. environ. microbiol. 69:7354-7363, 2003). only a small fraction (4%) of the 16s rrna gene sequences of the digester clone library corresponded to the planctomycetales div ...	2005	15812049
microscale process evaluation of recombinant biocatalyst libraries: application to baeyer-villiger monooxygenase catalysed lactone synthesis.	microscale processing techniques are rapidly emerging as a cost- effective means for parallel experimentation and hence the evaluation of large libraries of recombinant biocatalysts. in this work, the potential of an automated microscale process is demonstrated in a linked sequence of operations comprising fermentation, enzyme induction and bioconversion using three whole-cell biocatalysts each expressing cyclohexanone monoxygenase (chmo). the biocatalysts, escherichia coli top 10 [pqr239], e. c ...	2005	16208497
detection of and response to signals involved in host-microbe interactions by plant-associated bacteria.	diverse interactions between hosts and microbes are initiated by the detection of host-released chemical signals. detection of these signals leads to altered patterns of gene expression that culminate in specific and adaptive changes in bacterial physiology that are required for these associations. this concept was first demonstrated for the members of the family rhizobiaceae and was later found to apply to many other plant-associated bacteria as well as to microbes that colonize human and anima ...	2005	15755957
alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome p450 encoded on the nifd excision element of anabaena 7120.	alkanes have been hypothesized to act as universal inducers of bacterial cytochrome p450 gene expression. we tested this hypothesis on an unusual p450 gene (cyp110) found on a conserved 11 kilobase episomal dna element of unknown function found in filamentous cyanobacteria. we also monitored the binding of potential substrates to the p450 protein and explored the distribution of p450 protein in vegetative cells and nitrogen-fixing heterocysts using immuno-electron microscopy.	2005	15790415
[use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil].	oil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as acinetobacter calcoaceticus k-4, nocardia vaceinii k-8, rhodococcus erythropolis ek-1, and mycobacterium sp. k-2. it was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. it was also found that water polluted with 100 mg/l oil could be purified with rhodococcus erythropolis ek-1 and nocardia vaceinii k-8 cells im ...	2005	15810734
biocatalytic production of perillyl alcohol from limonene by using a novel mycobacterium sp. cytochrome p450 alkane hydroxylase expressed in pseudomonas putida.	a number of oxygenated monoterpenes present at low concentrations in plant oils have anticarcinogenic properties. one of the most promising compounds in this respect is (-)-perillyl alcohol. since this natural product is present only at low levels in a few plant oils, an alternative, synthetic source is desirable. screening of 1,800 bacterial strains showed that many alkane degraders were able to specifically hydroxylate l-limonene in the 7 position to produce enantiopure (-)-perillyl alcohol. t ...	2005	15811996
formation of trans fatty acids is not involved in growth-linked membrane adaptation of pseudomonas putida.	fatty acid compositions in growing and resting cells of several strains of pseudomonas putida (p8, nctc 10936, and kt 2440) were studied, with a focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. the fatty acid compositions of the strains were very similar under comparable growth conditions, but surprisingly, and contrary to earlier reports, trans fatty acids were not found in either exponentially growing cells or stationary-phase cells. during th ...	2005	15812020
species-level identification of isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex by sequence analysis of the 16s-23s rrna gene spacer region.	the species acinetobacter calcoaceticus, a. baumannii, genomic species 3, and genomic species 13tu included in the acinetobacter calcoaceticus-acinetobacter baumannii complex are genetically highly related and difficult to distinguish phenotypically. except for a. calcoaceticus, they are all important nosocomial species. in the present study, the usefulness of the 16s-23s rrna gene intergenic spacer (its) sequence for the differentiation of (genomic) species in the a. calcoaceticus-a. baumannii ...	2005	15814977
sulfoacetaldehyde is excreted quantitatively by acinetobacter calcoaceticus sw1 during growth with taurine as sole source of nitrogen.	eighteen enrichment cultures with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions were all successful, and 24 pure cultures were obtained. only three of the cultures yielded an inorganic product, sulfate, from the sulfonate moiety of taurine, and the others were presumed to yield organosulfonates. sulfoacetate, known from rhodopseudomonas palustris cga009 under these conditions, was not detected in any culture, but sulfoacetaldehyde (as a hydrazo ...	2005	15817795
structural modifications and antimicrobial activity of n-cycloalkenyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles.	a series of n-cycloalkenyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 5a-j, n-cycloalkyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 8a-e, and n-alkyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 8f-h, were synthesized and tested for in vitro antibacterial and antifungal activities against four gram-positive and five gram-negative bacteria (bacillus subtilis 6633, enterococcus faecalis 29212, staphylococcus aureus 6538, staphylococcus aureus 25923, escherichia coli 25922, acinetobact ...	2005	15848203
changes in antibiotic resistance of the most common gram-negative bacteria isolated in intensive care units.	we studied the changes in antibiotic resistance of the most common gram-negative bacteria isolated in the intensive care units at our hospital in 2000 and 2002. bacterial identification was performed by use of the vitek 60 analyser, and antibiotic susceptibilities were tested by the vitek 60 analyser and the disk diffusion agar method. the bacteria isolated most frequently were pseudomonas aeruginosa (132 strains in 2000 and 106 in 2002), acinetobacter calcoaceticus (98 and 109 strains, respecti ...	2005	15890431
catabolism of benzoate and phthalate in rhodococcus sp. strain rha1: redundancies and convergence.	genomic and proteomic approaches were used to investigate phthalate and benzoate catabolism in rhodococcus sp. strain rha1, a polychlorinated biphenyl-degrading actinomycete. sequence analyses identified genes involved in the catabolism of benzoate (ben) and phthalate (pad), the uptake of phthalate (pat), and two branches of the beta-ketoadipate pathway (catrabc and pcajihgblfr). the regulatory and structural ben genes are separated by genes encoding a cytochrome p450. the pad and pat genes are ...	2005	15937168
gene conversion tracts associated with crossovers in rhizobium etli.	gene conversion has been defined as the nonreciprocal transfer of information between homologous sequences. despite its broad interest for genome evolution, the occurrence of this mechanism in bacteria has been difficult to ascertain due to the possible occurrence of multiple crossover events that would mimic gene conversion. in this work, we employ a novel system, based on cointegrate formation, to isolate gene conversion events associated with crossovers in the nitrogen-fixing bacterium rhizob ...	2005	15937174
real-time monitoring of enzymatic dna hydrolysis by electrospray ionization mass spectrometry.	a fast and direct method for the monitoring of enzymatic dna hydrolysis was developed using electrospray ionization mass spectrometry. we incorporated the use of a robotic chip-based electrospray ionization source for increased reproducibility and throughput. the mass spectrometry method allows the detection of dna fragments and intact non-covalent protein-dna complexes in a single experiment. we used the method to monitor in real-time single-stranded (ss) dna hydrolysis by colicin e9 dnase and ...	2005	15956101
worldwide disseminated arma aminoglycoside resistance methylase gene is borne by composite transposon tn1548.	the arma (aminoglycoside resistance methylase) gene, which confers resistance to 4,6-disubstituted deoxystreptamines and fortimicin, was initially found in klebsiella pneumoniae bm4536 on incl/m plasmid pip1204 of ca. 90 kb which also encodes the extended-spectrum beta-lactamase ctx-m-3. thirty-four enterobacteria from various countries that were likely to produce a ctx-m enzyme since they were more resistant to cefotaxime than to ceftazidime were studied. the arma gene was detected in 12 clinic ...	2005	15980373
degradation of cyanophycin by sedimentibacter hongkongensis strain ki and citrobacter amalonaticus strain g isolated from an anaerobic bacterial consortium.	using a combination of various enrichment techniques, the strictly anaerobic, gram-positive, endospore-forming bacterium sedimentibacter hongkongensis strain ki as revealed by 16s rrna analysis and the gram-negative enterobacterium citrobacter amalonaticus strain g as revealed by physiological tests were isolated from an anaerobic cyanophycin (cgp)-degrading bacterial consortium. s. hongkongensis strain ki is the first anaerobic bacterium with the ability to hydrolyze cgp to beta-asp-arg and bet ...	2005	16000772
abundance of three bacterial populations in selected streams.	the population sizes of three bacterial species, acinetobacter calcoaceticus, burkholderia cepacia, and pseudomonas putida, were examined in water and sediment from nine streams in different parts of the united states using fluorescent in situ hybridization (fish). population sizes were determined from three sites (upstream, midstream, and downstream) in each stream to compare differences in the occurrence and distribution of the species within each stream and among streams. physical and chemica ...	2005	16003475
increasing stability of water-soluble pqq glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface.	water-soluble quinoprotein glucose dehydrogenase (pqqgdh-b) from acinetobacter calcoaceticus has a great potential for application as a glucose sensor constituent. because this enzyme shows no activity in its monomeric form, correct quaternary structure is essential for the formation of active enzyme. we have previously reported on the increasing of the stability of pqqgdh-b by preventing the subunit dissociation. previous studies were based on decreasing the entropy of quaternary structure diss ...	2005	15715904
epr studies of chlorocatechol 1,2-dioxygenase: evidences of iron reduction during catalysis and of the binding of amphipatic molecules.	chlorocatechol 1,2-dioxygenase from pseudomonas putida (pp 1,2-ccd) is a dioxygenase responsible for ring cleavage during the degradation of recalcitrant aromatic compounds. we determined the zero-field splitting of the fe(iii) cofactor (\|d\| = 1.3 +/- 0.2 cm(-1)) by electron paramagnetic resonance (epr) experiments that along with other structural data allowed us to infer the fe(iii) coordination environment. the epr spectrum of the ion shows a significantly decrease of the g = 4.3 resonance upo ...	2005	15722436
development of a novel glucose enzyme fuel cell system employing protein engineered pqq glucose dehydrogenase.	glucose dehydrogenase harboring pyrroloquinoline quinone as the prosthetic group (pqqgdh) from acinetobacter calcoaceticus is an ideal enzyme for the anode of biofuel cell, because of its oxygen insensitivity and high catalytic efficiency. however, the application of pqqgdh for the bioanode is inherently limited because of its instability. using ser415cys mutant whose stability was greatly improved, we constructed the biofuel cell system employing the engineered pqqgdh as the bioanode enzyme and ...	2005	15741089
quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia.	a quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-rflp) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. the real-time-t-rflp assay was developed by incorporating the quantitative feature of real-time pcr and the fingerprinting feature of t-rflp analysis. the assay was validated by using a model microbial community containing three pure strains, an escherich ...	2005	15746346
manual and automated instrumentation for identification of enterobacteriaceae and other aerobic gram-negative bacilli.	identification of gram-negative bacilli, both enteric and nonenteric, by conventional methods is not realistic for clinical microbiology laboratories performing routine cultures in today's world. the use of commercial kits, either manual or automated, to identify these organisms is a common practice. the advent of rapid or "spot" testing has eliminated the need for some commonly isolated organisms to be identified with the systems approach. commercially available systems provide more in-depth id ...	2005	15653824
mechanism of lipid-body formation in prokaryotes: how bacteria fatten up.	neutral lipid accumulation is frequently observed in some gram-negative prokaryotes like acinetobacter sp. and most actinomycetes, including the pathogenic mycobacterium tuberculosis and antibiotic producing streptomycetes. we examined the formation of wax ester- and triacylglycerol (tag)-bodies in acinetobacter calcoaceticus and rhodococcus opacus using microscopic, immunological and biophysical methods. a general model for prokaryotic lipid-body formation is proposed, clearly differing from th ...	2005	15661001
thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme a:diacylglycerol acyltransferase of acinetobacter sp. strain adp1.	the bifunctional wax ester synthase/acyl coenzyme a (acyl-coa):diacylglycerol acyltransferase (ws/dgat) from acinetobacter sp. strain adp1 (formerly acinetobacter calcoaceticus adp1) mediating the biosyntheses of wax esters and triacylglycerols was used for the in vivo and in vitro biosynthesis of thio wax esters and dithio wax esters. for in vitro biosynthesis, 5'his(6)ws/dgat comprising an n-terminal his(6) tag was purified from the soluble protein fraction of escherichia coli rosetta(de3)plys ...	2005	15691932
physiological conditions conducive to high cyanophycin content in biomass of acinetobacter calcoaceticus strain adp1.	the effects of the inorganic medium components, the initial ph, the incubation temperature, the oxygen supply, the carbon-to-nitrogen ratio, and chloramphenicol on the synthesis of cyanophycin (cgp) by acinetobacter calcoaceticus strain adp1 were studied in a mineral salts medium containing sodium glutamate and ammonium sulfate as carbon and nitrogen sources, respectively. variation of all these factors resulted in maximum cgp contents of only about 3.5% (wt/wt) of the cell dry matter (cdm), and ...	2005	15691941
transcriptional organization of genes for protocatechuate and quinate degradation from acinetobacter sp. strain adp1.	quinate and protocatechuate are both abundant plant products and can serve, along with a large number of other aromatic or hydroaromatic compounds, as growth substrates for acinetobacter sp. strain adp1. the respective genes are part of the chromosomal dca-pca-qui-pob-hca cluster encoding these pathways. the adjacent pca and qui gene clusters, which encode enzymes for protocatechuate breakdown via the beta-ketoadipate pathway and for the conversion of quinate or shikimate to protocatechuate, res ...	2005	15691962
multidrug-resistant acinetobacter extremity infections in soldiers.	war wound infection and osteomyelitis caused by multidrug-resistant (mdr) acinetobacter species have been prevalent during the 2003-2005 military operations in iraq. twenty-three soldiers wounded in iraq and subsequently admitted to our facility from march 2003 to may 2004 had wound cultures positive for acinetobacter calcoaceticus-baumannii complex. eighteen had osteomyelitis, 2 burn infection, and 3 deep wound infection. primary therapy for these infections was directed antimicrobial agents fo ...	2005	16102310
streptomyces pharetrae sp. nov., isolated from soil from the semi-arid karoo region.	an actinomycete, strain cza14t, was isolated from worcester in the western cape province of south africa. based on rapid genus identification, 16s-rdna sequence similarities and chemotaxonomy, strain cza14t was identified as a member of the genus streptomyces. it exhibited weak antibiosis against bacillus coagulans atcc 7050t, mycobacterium aurum a+ and acinetobacter calcoaceticus c91. the results of physiological tests and analysis of the 16s-rdna sequence allowed for the differentiation of str ...	2005	16104349
development of a multilocus sequence typing scheme for characterization of clinical isolates of acinetobacter baumannii.	in this study a multilocus sequence typing (mlst) scheme for acinetobacter baumannii was developed and evaluated by using 40 clinical a. baumannii isolates recovered from outbreaks in spanish and german hospitals during the years 1990 to 2001, as well as isolates from other european hospitals and two dsmz reference strains of a. baumannii. for comparison, two isolates of acinetobacter species 13 (sensu tjernberg and ursing), two clinical isolates, and three dsmz strains of a. calcoaceticus (both ...	2005	16145081
characterization and changing minimum inhibitory concentration (mic) of acinetobacter species from a tertiary care setup.	128 isolates of acinetobacter species from admitted and outdoor patients were subjected to biotyping and resistotyping. resistance phenotype analysis included nine antibiotics and two betalactam inhibitor combination drugs. in 100 strains of acinetobacter spp. ciprofloxacin, amikacin, cefotaxime and cefepime minimum inhibitory concentration (mic) was done by agar dilution using nccls 2002 criteria. in forty-nine isolates mic level was determined by e-strip also. extended spectrum beta lactamase ...	2005	16749272
stereoselective esterase from pseudomonas putida ifo12996 reveals alpha/beta hydrolase folds for d-beta-acetylthioisobutyric acid synthesis.	esterase (est) from pseudomonas putida ifo12996 catalyzes the stereoselective hydrolysis of methyl dl-beta-acetylthioisobutyrate (dl-mati) to produce d-beta-acetylthioisobutyric acid (dat), serving as a key intermediate for the synthesis of angiotensin-converting enzyme inhibitors. the est gene was cloned and expressed in escherichia coli; the recombinant protein is a non-disulfide-linked homotrimer with a monomer molecular weight of 33,000 in both solution and crystalline states, indicating tha ...	2005	16321951
protamylasse, a residual compound of industrial starch production, provides a suitable medium for large-scale cyanophycin production.	protamylasse is a residual compound occurring during the industrial production of starch from potatoes. it contains a variety of nutrients and all necessary minerals and could be used as a carbon, nitrogen, and energy source for the growth of bacteria and also for cyanophycin (cgp) biosynthesis. media containing protamylasse as the sole compound diluted only in water were therefore examined for their suitability in cgp production. among various bacterial strains investigated in this study, a rec ...	2005	16332748
detection and isolation of ultrasmall microorganisms from a 120,000-year-old greenland glacier ice core.	the abundant microbial population in a 3,043-m-deep greenland glacier ice core was dominated by ultrasmall cells (<0.1 microm3) that may represent intrinsically small organisms or starved, minute forms of normal-sized microbes. in order to examine their diversity and obtain isolates, we enriched for ultrasmall psychrophiles by filtering melted ice through filters with different pore sizes, inoculating anaerobic low-nutrient liquid media, and performing successive rounds of filtrations and recult ...	2005	16332755
hydroxylation of the herbicide isoproturon by fungi isolated from agricultural soil.	several asco-, basidio-, and zygomycetes isolated from an agricultural field were shown to be able to hydroxylate the phenylurea herbicide isoproturon [n-(4-isopropylphenyl)-n',n'-dimethylurea] to n-(4-(2-hydroxy-1-methylethyl)phenyl)-n',n'-dimethylurea and n-(4-(1-hydroxy-1-methylethyl)phenyl)-n',n'-dimethylurea. bacterial metabolism of isoproturon has previously been shown to proceed by an initial demethylation to n-(4-isopropylphenyl)-n'-methylurea. in soils, however, hydroxylated metabolites ...	2005	16332769
fatty acid and hydroxy acid adaptation in three gram-negative hydrocarbon-degrading bacteria in relation to carbon source.	the lipids of three gram-negative bacteria, acinetobacter calcoaceticus, marinobacter aquaeolei, and pseudomonas oleovorans grown on mineral media supplemented with ammonium acetate or hydrocarbons, were isolated, purified, and their structures determined. three pools of lipids were isolated according to a sequential procedure: unbound lipids extracted with organic solvents, comprising metabolic lipids and the main part of membrane lipids, oh--labile lipids (mainly ester-bound in the lipopolysac ...	2005	16477811
health considerations regarding horizontal transfer of microbial transgenes present in genetically modified crops.	the potential effects of horizontal gene transfer on human health are an important item in the safety assessment of genetically modified organisms. horizontal gene transfer from genetically modified crops to gut microflora most likely occurs with transgenes of microbial origin. the characteristics of microbial transgenes other than antibiotic-resistance genes in market-approved genetically modified crops are reviewed. these characteristics include the microbial source, natural function, function ...	2005	16489267
identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases.	selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (ahs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. first, substrate range mutants of the pseudomonas putida gpo1 and alcanivorax borkumensis ap1 medium-chain-length ahs were obtained by selection experiments with a specially constructed host. in all mutants able to oxidize alkanes longer than c13, w55 (in the case of p. put ...	2005	15601691
impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene.	with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ...	2005	15626648
impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene.	with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ...	2005	15626648
sequencing of the rpob gene and flanking spacers for molecular identification of acinetobacter species.	acinetobacter species are defined on the basis of several phenotypic characters, results of dna-dna homology, and more recently, similarities or dissimilarities in 16s rrna gene sequences. however, the 16s rrna gene is not polymorphic enough to clearly distinguish all acinetobacter species. we used an rna polymerase beta-subunit gene (rpob)-based identification scheme for the delineation of species within the genus acinetobacter, and towards that end, we determined the complete rpob gene and fla ...	2006	16517861
low-intensity photosensitization may enhance reca production.	three bacterial strains-escherichia coli, acinetobacter calcoaceticus, and the a. calcoaceticus reca- mutant-underwent photosensitization by a low-concentration (0.73 micromol/l) tetramethyl pyridyl porphine (a cationic hydrophylic photosensitizer) and a 4-j/cm2 dose of 407 to 420 nm blue light. the viability of the first two strains decreased by approximately 60%. and that of the reca- strain decreased by 90%. increasing the amount of photosensitizer to 14.6 micromol/l at the same dose of blue ...	2006	16528464
sequence and organization of pmac, an acinetobacter baumannii plasmid harboring genes involved in organic peroxide resistance.	acinetobacter baumannii 19606 harbors pmac, a 9540-bp plasmid that contains 11 predicted open-reading frames (orfs). cloning and transformation experiments using acinetobacter calcoaceticus bd413 mapped replication functions within a region containing four 21-bp direct repeats (ori) and orf 1, which codes for a predicted replication protein. subcloning and tri-parental mating experiments mapped mobilization functions to the product of orf 11 and an adjacent predicted orit. three orfs code for pr ...	2006	16530832
antagonistic interactions between honey bee bacterial symbionts and implications for disease.	honey bees, apis mellifera, face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. one route by which honey bees and other insects might combat disease is through the shielding effects of their microbial symbionts. bees carry a diverse assemblage of bacteria, very few of which appear to be pathogenic. here we explore the inhibitory effects of these resident bacteria against the primary bacterial pathogen of honey bees, ...	2006	16551367
16s rrna gene sequencing versus the api 20 ne system and the vitek 2 id-gnb card for identification of nonfermenting gram-negative bacteria in the clinical laboratory.	over a period of 26 months, we have evaluated in a prospective fashion the use of 16s rrna gene sequencing as a means of identifying clinically relevant isolates of nonfermenting gram-negative bacilli (non-pseudomonas aeruginosa) in the microbiology laboratory. the study was designed to compare phenotypic with molecular identification. results of molecular analyses were compared with two commercially available identification systems (api 20 ne, vitek 2 fluorescent card; biomérieux, marcy l'etoil ...	2006	16597863
nosocomial bacteremia due to an as yet unclassified acinetobacter genomic species 17-like strain.	we describe a case of bacteremia due to an as yet unclassified acinetobacter genomic species 17-like strain. the recognition of this microorganism as non-acinetobacter baumannii may have important epidemiological implications, as it relieves the hospital of the implementation of barrier precautions for patients infected or colonized as may be necessary with a multiresistant a. baumannii epidemic.	2006	16597902
culturability of stream bacteria assessed at the assemblage and population levels.	lotic bacterial communities can be examined at multiple levels: from the assemblage level to populations of individual species. in stream environments, as in many other systems, the percentage of bacteria that are culturable is quite low. in this study, the culturability of the overall bacterial assemblage, as well as the culturability of three common species (acinetobacter calcoaceticus, burkholderia cepacia, and pseudomonas putida), was determined in samples collected from four streams on thre ...	2006	16598638
rapid detection of brucella spp. in blood cultures by fluorescence in situ hybridization.	brucellosis is a severe systemic disease in humans. we describe a new 16s rrna-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of brucella and that can be applied directly to positive blood cultures.	2006	16672413
proteomic insights into metabolic adaptations in alcanivorax borkumensis induced by alkane utilization.	alcanivorax borkumensis is a ubiquitous marine petroleum oil-degrading bacterium with an unusual physiology specialized for alkane metabolism. this "hydrocarbonoclastic" bacterium degrades an exceptionally broad range of alkane hydrocarbons but few other substrates. the proteomic analysis presented here reveals metabolic features of the hydrocarbonoclastic lifestyle. specifically, hexadecane-grown and pyruvate-grown cells differed in the expression of 97 cytoplasmic and membrane-associated prote ...	2006	16707669
abundant and diverse fungal microbiota in the murine intestine.	enteric microbiota play a variety of roles in intestinal health and disease. while bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. this study utilized a culture-independent method termed oligonucleotide fingerprinting of rrna genes (ofrg) to describe the compositions of fungal and bacterial rrna genes from small and large intestines (tissue and luminal contents) of restricted-flora and specific-pathogen-free mice. ofrg ...	2006	16391120
naturally transformable acinetobacter sp. strain adp1 belongs to the newly described species acinetobacter baylyi.	genotypic and phenotypic analyses were carried out to clarify the taxonomic position of the naturally transformable acinetobacter sp. strain adp1. transfer tdna-pcr fingerprinting, 16s rrna gene sequence analysis, and selective restriction fragment amplification (amplified fragment length polymorphism analysis) indicate that strain adp1 and a second transformable strain, designated 93a2, are members of the newly described species acinetobacter baylyi. transformation assays demonstrate that the a ...	2006	16391138
identification of anthranilate and benzoate metabolic operons of pseudomonas fluorescens and functional characterization of their promoter regions.	in an effort to identify alternate recombinant gene expression systems in pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antabc) and the benzoate operon (benabcd).	2006	16396686
microbiology of airway disease in a cohort of patients with cystic fibrosis.	recent reports document an increasing incidence of new gram-negative pathogens such as stenotrophomonas maltophilia and alcaligenes xylosoxidans isolated from patients with cystic fibrosis, along with an increase in common gram-negative pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex. furthermore, the increase in multidrug-resistance of such organisms makes the therapeutic management of these patients more problematic. therefore, careful isolation and identification, an ...	2006	16405721
nosocomial pneumonia : rationalizing the approach to empirical therapy.	nosocomial pneumonia or hospital-acquired pneumonia (hap) causes considerable morbidity and mortality. it is the second most common nosocomial infection and the leading cause of death from hospital-acquired infections. in 1996 the american thoracic society (ats) published guidelines for empirical therapy of hap. this review focuses on the literature that has appeared since the ats statement. early diagnosis of hap and its etiology is crucial in guiding empirical therapy. since 1996, it has becom ...	2006	16409013
novel organic hydroperoxide-sensing and responding mechanisms for ohrr, a major bacterial sensor and regulator of organic hydroperoxide stress.	xanthomonas campestris pv. phaseoli ohrr belongs to a major family of multiple-cysteine-containing bacterial organic hydroperoxide sensors and transcription repressors. site-directed mutagenesis and subsequent in vivo functional analyses revealed that changing any cysteine residue to serine did not alter the ability of ohrr to bind to the p1 ohrr-ohr promoter but drastically affected the organic hydroperoxide-sensing and response mechanisms of the protein. xanthomonas ohrr requires two cysteine ...	2006	16452421
the thin pili of acinetobacter sp. strain bd413 mediate adhesion to biotic and abiotic surfaces.	two structurally different appendages, thin and thick pili, are found in members of the genus acinetobacter. the presence of pilus structures correlates with different phenotypes, such as adherence to surfaces, a trait not only observed in pathogenic acinetobacter species, as well as motility. however, their distinct individual roles were unknown. to characterize the role of different pili in the physiology of acinetobacter, we isolated the thin pili from the cell surface of acinetobacter sp. st ...	2006	16461692
engineering the genotype of acinetobacter sp. strain adp1 to enhance biosynthesis of cyanophycin.	to study the importance of arginine provision and phosphate limitation for synthesis and accumulation of cyanophycin (cgp) in acinetobacter sp. strain adp1, genes encoding the putative arginine regulatory protein (argr) and the arginine succinyltransferase (asta) were inactivated, and the effects of these mutations on cgp synthesis were analyzed. the inactivation of these genes resulted in a 3.5- or 7-fold increase in cgp content, respectively, when the cells were grown on glutamate. knockout mu ...	2006	16461694
pyrroloquinoline quinone-dependent dehydrogenases from ketogulonicigenium vulgare catalyze the direct conversion of l-sorbosone to l-ascorbic acid.	a novel enzyme, l-sorbosone dehydrogenase 1 (sndh1), which directly converts l-sorbosone to l-ascorbic acid (l-aa), was isolated from ketogulonicigenium vulgare dsm 4025 and characterized. this enzyme was a homooligomer of 75-kda subunits containing pyrroloquinoline quinone (pqq) and heme c as the prosthetic groups. two isozymes of sndh, sndh2 consisting of 75-kda and 55-kda subunits and sndh3 consisting of 55-kda subunits, were also purified from the bacterium. all of the sndhs produced l-aa, a ...	2006	16461703
a constitutively expressed, truncated umudc operon regulates the reca-dependent dna damage induction of a gene in acinetobacter baylyi strain adp1.	in response to environmentally caused dna damage, sos genes are up-regulated due to reca-mediated relief of lexa repression. in escherichia coli, the sos umudc operon is required for dna damage checkpoint functions and for replicating damaged dna in the error-prone process called sos mutagenesis. in the model soil bacterium acinetobacter baylyi strain adp1, however, the content, regulation, and function of the umudc operon are unusual. the umuc gene is incomplete, and a remnant of an isehe3-like ...	2006	16751513
energetics and surface properties of pseudomonas putida dot-t1e in a two-phase fermentation system with 1-decanol as second phase.	the solvent-tolerant strain pseudomonas putida dot-t1e was grown in batch fermentations in a 5-liter bioreactor in the presence and absence of 10% (vol/vol) of the organic solvent 1-decanol. the growth behavior and cellular energetics, such as the cellular atp content and the energy charge, as well as the cell surface hydrophobicity and charge, were measured in cells growing in the presence and absence of 1-decanol. although the cells growing in the presence of 1-decanol showed an about 10% redu ...	2006	16751536
crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding.	the soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. an efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. similar cross-seeding protocols to grow crystals of homologues from escherichia coli and streptomyces coelicolor are described.	2006	16754970
design and evaluation of 16s rrna sequence based oligonucleotide probes for the detection and quantification of comamonas testosteroni in mixed microbial communities.	the beta-proteobacterial species comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation. the ability to detect and quantify members of this species in mixed microbial communities thus may be desirable.	2006	16772028
cytosolic triacylglycerol biosynthetic pathway in oilseeds. molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase.	triacylglycerols (tags) are the most important storage form of energy for eukaryotic cells. tag biosynthetic activity was identified in the cytosolic fraction of developing peanut (arachis hypogaea) cotyledons. this activity was naf insensitive and acyl-coenzyme a (coa) dependent. acyl-coa:diacylglycerol acyltransferase (dgat) catalyzes the final step in tag biosynthesis that acylates diacylglycerol to tag. soluble dgat was identified from immature peanuts and purified by conventional column chr ...	2006	16798944
acinetobacter skin colonization of us army soldiers.	to evaluate whether skin colonization with acinetobacter calcoaceticus-baumannii complex exists in a population of healthy, nondeployed us army soldiers and, if present, how it might relate to the infections seen in current war casualties.	2006	16807838
soluble aldose sugar dehydrogenase from escherichia coli: a highly exposed active site conferring broad substrate specificity.	a water-soluble aldose sugar dehydrogenase (asd) has been purified for the first time from escherichia coli. the enzyme is able to act upon a broad range of aldose sugars, encompassing hexoses, pentoses, disaccharides, and trisaccharides, and is able to oxidize glucose to gluconolactone with subsequent hydrolysis to gluconic acid. the enzyme shows the ability to bind pyrroloquinoline quinone (pqq) in the presence of ca2+ in a manner that is proportional to its catalytic activity. the x-ray struc ...	2006	16864586
how long do nosocomial pathogens persist on inanimate surfaces? a systematic review.	inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces.	2006	16914034
knockout and overexpression of pyrroloquinoline quinone biosynthetic genes in gluconobacter oxydans 621h.	in gluconobacter oxydans, pyrroloquinoline quinone (pqq) serves as the cofactor for various membrane-bound dehydrogenases that oxidize sugars and alcohols in the periplasm. proteins for the biosynthesis of pqq are encoded by the pqqabcde gene cluster. our reverse transcription-pcr and promoter analysis data indicated that the pqqa promoter represents the only promoter within the pqqabcde cluster of g. oxydans 621h. pqq overproduction in g. oxydans was achieved by transformation with the plasmid- ...	2006	16936032
identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in mycobacterium tuberculosis under stress.	mycobacterium tuberculosis under stress stores triacylglycerol (tg). there are 15 genes in m. tuberculosis that belong to a novel family of tg synthase genes (tgs), but it is not known which of them is responsible for this accumulation of tg. in this paper, it is reported that m. tuberculosis h37rv accumulated tg under acidic, static or hypoxic growth conditions, or upon treatment with no, whereas tg accumulation was drastically reduced in the tgs1 (rv3130c) disrupted mutant. complementation wit ...	2006	16946266
microbial population dynamics associated with crude-oil biodegradation in diverse soils.	soil bacterial population dynamics were examined in several crude-oil-contaminated soils to identify those organisms associated with alkane degradation and to assess patterns in microbial response across disparate soils. seven soil types obtained from six geographically distinct areas of the united states (arizona, oregon, indiana, virginia, oklahoma, and montana) were used in controlled contamination experiments containing 2% (wt/wt) crude oil spiked with [1-(14)c]hexadecane. microbial populati ...	2006	16957258
impact of acinetobacter infection on the mortality of burn patients.	acinetobacter calcoaceticus-baumannii complex (acb) is recognized as an important cause of nosocomial infections. although acb can be associated with multidrug resistance, its impact on mortality in burn patients has not been fully elucidated.	2006	17000400
identification of novel benzoylformate decarboxylases by growth selection.	a growth selection system was established using pseudomonas putida, which can grow on benzaldehyde as the sole carbon source. these bacteria presumably metabolize benzaldehyde via the beta-ketoadipate pathway and were unable to grow in benzoylformate-containing selective medium, but the growth deficiency could be restored by expression in trans of genes encoding benzoylformate decarboxylases. the selection system was used to identify three novel benzoylformate decarboxylases, two of them origina ...	2006	17012586
cultivation-independent examination of horizontal transfer and host range of an incp-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere.	the host range and transfer frequency of an incp-1 plasmid (pkjk10) among indigenous bacteria in the barley rhizosphere was investigated. a new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16s rrna gene classification was used. indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16s rrna genes from the sorted cells. the host range of th ...	2006	17021220
rapid evolution of diminished transformability in acinetobacter baylyi.	the reason for genetic exchange remains a crucial question in evolutionary biology. acinetobacter baylyi strain adp1 is a highly competent and recombinogenic bacterium. we compared the parallel evolution of wild-type and engineered noncompetent lineages of a. baylyi in the laboratory. if transformability were to result in an evolutionary benefit, it was expected that competent lineages would adapt more rapidly than noncompetent lineages. instead, regardless of competency, lineages adapted to the ...	2006	17028281
[characteristics of war wound infection].	war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ...	2006	17048790
bacterial identification, clinical significance, and antimicrobial susceptibilities of acinetobacter ursingii and acinetobacter schindleri, two frequently misidentified opportunistic pathogens.	the species belonging to the acinetobacter genus are currently reported as opportunistic pathogens in hospitalized patients with underlying predispositions. however, except for the acinetobacter calcoaceticus-acinetobacter baumannii complex, the identification of other species is frequently unreliable, especially for acinetobacter ursingii and acinetobacter schindleri, newly described in 2001. thus, the clinical significance, phenotypic features, and antimicrobial susceptibilities of these two m ...	2006	17050816
identification of substrates of the mycobacterium tuberculosis proteasome.	the putative proteasome-associated proteins mpa (mycobaterium proteasomal atpase) and pafa (proteasome accessory factor a) of the human pathogen mycobacterium tuberculosis (mtb) are essential for virulence and resistance to nitric oxide. however, a direct link between the proteasome protease and mpa or pafa has never been demonstrated. furthermore, protein degradation by bacterial proteasomes in vitro has not been accomplished, possibly due to the failure to find natural degradation substrates o ...	2006	17082771
identification of rv3230c as the nadph oxidoreductase of a two-protein desa3 acyl-coa desaturase in mycobacterium tuberculosis h37rv.	desa3 is a membrane-bound stearoyl-coa delta(9)-desaturase that produces oleic acid, a precursor of mycobacterial membrane phospholipids and triglycerides. the sequence of desa3 is homologous with those of other membrane desaturases, including the presence of the eight-his motif proposed to bind the diiron center active site. this family of desaturases function as multicomponent complexes and thus require electron transfer proteins for efficient catalytic turnover. here we present evidence that ...	2006	17087501
constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc.	the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ...	2006	16156722
constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc.	the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ...	2006	16156722
a unique polypeptide from the c-terminus of the exocellular esterase of acinetobacter venetianus rag-1 modulates the emulsifying activity of the polymeric bioemulsifier apoemulsan.	an exocellular esterase from the oil-degrading acinetobacter venetianus rag-1 was previously shown to enhance the emulsification and emulsion stabilization properties of the amphipathic, aminopolysaccharide bioemulsifier, emulsan [bach h, berdichevsky y, gutnick d (2003) an exocellular protein from the oil-degrading microbe acinetobacter venetianus rag-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. appl environ microbiol 69:2608-15]. this enhancement was specific for ...	2006	16237525
isolation and characterization of a phenol-degrading bacterium from an industrial activated sludge.	this paper reports the successful isolation and characterization of a new phenol-degrading bacterium, strain edp3, from activated sludge. strain edp3 is a nonmotile, strictly aerobic, gram-negative, and short-rod or coccobacillary bacterium, which occurs singly, in pairs, or in clusters. 16s rrna gene sequence analysis revealed that strain edp3 belonged to the gamma group of proteobacteria, with a 97.0% identity to 16s rrna gene sequences of acinetobacter calcoaceticus. strain edp3 could aerobic ...	2006	16283294