| h(2)o(2)-forming nadh oxidase with diaphorase (cytochrome) activity from archaeoglobus fulgidus. | an enzyme exhibiting nadh oxidase (diaphorase) activity was isolated from the hyperthermophilic sulfate-reducing anaerobe archaeoglobus fulgidus. n-terminal sequence of the protein indicates that it is coded for by open reading frame af0395 in the a. fulgidus genome. the gene af0395 was cloned and its product was purified from escherichia coli. like the native nadh oxidase (noxa2), the recombinant noxa2 (rnoxa2) has an apparent molecular mass of 47 kda, requires flavin adenine dinucleotide for a ... | 2001 | 11717257 |
| the active component of the bioemulsifier alasan from acinetobacter radioresistens ka53 is an ompa-like protein. | the bioemulsifier of acinetobacter radioresistens ka53, referred to as alasan, is a high-molecular-weight complex of polysaccharide and protein. recently, one of the alasan proteins, with an apparent molecular mass of 45 kda, was purified and shown to constitute most of the emulsifying activity. the n-terminal sequence of the 45-kda protein showed high homology to an ompa-like protein from acinetobacter spp. in the research described here the gene coding for the 45-kda protein was cloned, sequen ... | 2002 | 11741856 |
| bacterial degradation of natural and synthetic rubber. | the degradation of natural rubber (nr), synthetic poly(cis-1,4-isoprene) (sr), and cross-linked nr (latex gloves) by gram-positive and gram-negative bacteria was analyzed by weight loss, gel permeation chromatography, and determination of the protein content. weight losses of 11-18% and an increase in protein up to 850 microg/ml after incubation of nocardia sp. dsmz43191, streptomyces coelicolor, streptomyces griseus, bacterial isolate 18a, acinetobacter calcoaceticus, and xanthomonas sp. with l ... | 2001 | 11749186 |
| duplicate copies of genes encoding methanesulfonate monooxygenase in marinosulfonomonas methylotropha strain tr3 and detection of methanesulfonate utilizers in the environment. | marinosulfonomonas methylotropha strain tr3 is a marine methylotroph that uses methanesulfonic acid (msa) as a sole carbon and energy source. the genes from m. methylotropha strain tr3 encoding methanesulfonate monooxygenase, the enzyme responsible for the initial oxidation of msa to formaldehyde and sulfite, were cloned and sequenced. they were located on two gene clusters on the chromosome of this bacterium. a 5.0-kbp hindiii fragment contained msma, msmb, and msmc, encoding the large and smal ... | 2002 | 11772638 |
| homology of escherichia coli r773 arsa, arsb, and arsc genes in arsenic-resistant bacteria isolated from raw sewage and arsenic-enriched creek waters. | the occurrence and diversity of the escherichia coli r773 ars operon were investigated among arsenic-resistant enteric and nonenteric bacteria isolated from raw sewage and arsenic-enriched creek waters. selected isolates from each creek location were screened for ars genes by colony hybridization and pcr. the occurrence of arsa, arsb, and arsc determined by low-stringency colony hybridization (31 to 53% estimated mismatch) was 81, 87, and 86%, respectively, for 84 bacteria isolated on arsenate- ... | 2002 | 11772637 |
| new degenerate cytophaga-flexibacter-bacteroides-specific 16s ribosomal dna-targeted oligonucleotide probes reveal high bacterial diversity in river taff epilithon. | river microbial communities play an important role in global nutrient cycles, and aggregated bacteria such as those in epilithic biofilms may be major contributors. in this study the bacterial diversity of river taff epilithon in south wales was investigated. a 16s ribosomal dna (rdna) clone library was constructed and analyzed by partial sequencing of 76 of 347 clones and hybridization with taxon-specific probes. the epilithon was found to be very diverse, with an estimated 59.6% of the bacteri ... | 2002 | 11772628 |
| cross-reaction between the genus-specific lipopolysaccharide antigen of chlamydia spp. and the lipopolysaccharides of porphyromonas gingivalis, escherichia coli o119 and salmonella newington: implications for diagnosis. | seven hybridoma clones, secreting monoclonal antibodies (mabs) against the genus-specific chlamydial lipopolysaccharide (lps) antigen were obtained after immunization of balb/c mice with formalin killed chlamydia psittaci. the antigen-binding properties of the mabs were characterized in different immunologic reactions with purified chlamydial elementary bodies and lps antigens from s- and r-forms of gram-negative bacteria. four mabs reacted with the heterologous lps antigens of salmonella r-muta ... | 2001 | 11750161 |
| rapid identification of staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. | a new fluorescence in situ hybridization (fish) method with peptide nucleic acid (pna) probes for identification of staphylococcus aureus directly from positive blood culture bottles that contain gram-positive cocci in clusters (gpcc) is described. the test (the s. aureus pna fish assay) is based on a fluorescein-labeled pna probe that targets a species-specific sequence of the 16s rrna of s. aureus. evaluations with 17 reference strains and 48 clinical isolates, including methicillin-resistant ... | 2002 | 11773123 |
| [intracellular accumulation of monomer precursors of polyphosphates and polyhydroxyalkanoates from acinetobacter calcoaceticus and escherichia coli]. | the formation of polyhydroxyalkanoates granules in anaerobically grown escherichia coli cells was found to be preceded by the intracellular accumulation of carbonic acids (predominantly, acetic acid), amounting to 9% of the cytosol. the intracellular concentration of acidic metabolites increased after the lyophilization of the bacterial biomass and decreased after its long-term storage (3.5-13.5 years). the decrease in the concentration of acidic metabolites is likely due to the dehydration of d ... | 2001 | 11785129 |
| identification of acinetobacter isolates from species belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex with monoclonal antibodies specific for o antigens of their lipopolysaccharides. | the unambiguous identification of acinetobacter strains, particularly those belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex, is often hindered by their close geno- and phenotypic relationships. in this study, monoclonal antibodies (mabs) against the o antigens of the lipopolysaccharides from strains belonging to the a. calcoaceticus-a. baumannii complex were generated after the immunization of mice with heat-killed bacteria and shown by enzyme immunoassays and wester ... | 2002 | 11777830 |
| characterization of antibiotic-resistant bacteria in rendered animal products. | antibiotics are used in food animal production to treat diseases and also to improve performance. antibiotics are not used on all farms, and antibiotic resistance is occasionally found on farms that do not use antibiotics. rendered animal protein products are often included in poultry feeds and could potentially serve as a source of antibiotic-resistant bacteria. one hundred sixty-five rendered animal protein products from cattle, poultry, and fish were aseptically collected from poultry feed mi ... | 2001 | 11785899 |
| the genetic organization and evolution of the broad host range mercury resistance plasmid psb102 isolated from a microbial population residing in the rhizosphere of alfalfa. | employing the biparental exogenous plasmid isolation method, conjugative plasmids conferring mercury resistance were isolated from the microbial community of the rhizosphere of field grown alfalfa plants. five different plasmids were identified, designated psb101-psb105. one of the plasmids, psb102, displayed broad host range (bhr) properties for plasmid replication and transfer unrelated to the known incompatibility (inc) groups of bhr plasmids incp-1, incw, incn and inca/c. nucleotide sequence ... | 2001 | 11812851 |
| charged amino acids conserved in the aromatic acid/h+ symporter family of permeases are required for 4-hydroxybenzoate transport by pcak from pseudomonas putida. | charged amino acids in the predicted transmembrane portion of pcak, a permease from pseudomonas putida that transports 4-hydroxybenzoate (4-hba), were required for 4-hba transport, and they were also required for p. putida to have a chemotactic response to 4-hba. an essential amino acid motif (dgxd) containing aspartate residues is located in the first transmembrane segment of pcak and is conserved in the aromatic acid/h+ symporter family of the major facilitator superfamily of transporters. | 2002 | 11844776 |
| nature, nomenclature and taxonomy of obligate methanol utilizing strains. | in a screening program, a number of different bacterial strains with the ability to utilize methanol as a sole carbon and energy source were isolated and described. they are well known methanol utilizing genera pseudomonas, klebsiella, micrococcus, methylomonas or, on the contrary, the new, unknown genera and species of methylotrophic bacteria. in the last category, acinetobacter and alcaligenes are the new reported genera of organisms able to use methanol as a sole carbon and energy source. the ... | 1999 | 11845445 |
| in-vitro interaction of azithromycin and fluoroquinolones against gram-positive and gram-negative bacteria. | objective: to determine the combined in-vitro effects of azithromycin plus the fluoroquinolone ofloxacin or lomefloxacin against gram-positive and gram-negative bacteria. methods: fractional inhibitory (fic) and fractional bactericidal concentration indices of azithromycin and the fluoroquinolone were determined using a microtiter-checkerboard method. clinical isolates of staphylococcus aureus, streptococcus pneumoniae, neisseria gonorrhoeae, escherichia coli, klebsiella pneumoniae, pseudomonas ... | 1996 | 11866755 |
| wax ester production from n-alkanes by acinetobacter sp. strain m-1: ultrastructure of cellular inclusions and role of acyl coenzyme a reductase. | acinetobacter sp. strain m-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. the contribution of acyl-coa reductase to wax ester synthesis was evaluated ... | 2002 | 11872467 |
| comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods. | a 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees c and transfer to 44 +/- 1 degrees c for 22 h) using fecal coliform agar (fca) was compared with the 24-h standardized violet red bile lactose agar (vrbl) method. fca and vrbl have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as hafnia alvei, which could form typical colonies on fca and vrbl. recovery of cold-stressed ... | 2002 | 11916678 |
| the active partition gene incc of incp plasmids is required for stable maintenance in a broad range of hosts. | plasmids of incompatibility group p (incp) are capable of replication and stable inheritance in a wide variety of gram-negative bacteria. three determinants of incp plasmids are components of an active partition locus that is predicted to function in the segregation of plasmid copies to daughter cells. these determinants are incc, which codes for a member of the para family of partition atpases; korb, which specifies a dna-binding protein that also functions as a global transcriptional repressor ... | 2002 | 11872733 |
| molecular method to assess the diversity of burkholderia species in environmental samples. | in spite of the importance of many members of the genus burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. the aim of this work was the development of soil dna-based pcr-denaturing gradient gel electrophoresis (dgge), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the burkholderia diversity in soil samples. primers specific for the genus burkholderia were developed bas ... | 2002 | 11916673 |
| functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. | we have cloned homologs of the pseudomonas putida gpo1 alkane hydroxylase from pseudomonas aeruginosa pao1, pseudomonas fluorescens cha0, alcanivorax borkumensis ap1, mycobacterium tuberculosis h37rv, and prauserella rugosa nrrl b-2295. sequence comparisons show that the level of protein sequence identity between the homologs is as low as 35%, and that the pseudomonas alkane hydroxylases are as distantly related to each other as to the remaining alkane hydroxylases. based on the observation that ... | 2002 | 11872725 |
| biofilms: survival mechanisms of clinically relevant microorganisms. | though biofilms were first described by antonie van leeuwenhoek, the theory describing the biofilm process was not developed until 1978. we now understand that biofilms are universal, occurring in aquatic and industrial water systems as well as a large number of environments and medical devices relevant for public health. using tools such as the scanning electron microscope and, more recently, the confocal laser scanning microscope, biofilm researchers now understand that biofilms are not unstru ... | 2002 | 11932229 |
| benzene-free synthesis of adipic acid. | strains of escherichia coli were constructed and evaluated that synthesized cis,cis-muconic acid from d-glucose under fed-batch fermentor conditions. chemical hydrogenation of the cis,cis-muconic acid in the resulting fermentation broth has also been examined. biocatalytic synthesis of adipic acid from glucose eliminates two environmental concerns characteristic of industrial adipic acid manufacture: use of carcinogenic benzene and benzene-derived chemicals as feedstocks and generation of nitrou ... | 2002 | 11934286 |
| molecular cloning and functional expression of d-sorbitol dehydrogenase from gluconobacter suboxydans if03255, which requires pyrroloquinoline quinone and hydrophobic protein sldb for activity development in e. coli. | the slda gene that encodes the d-sorbitol dehydrogenase (sldh) from gluconobacter suboxydans ifo 3255 was cloned and sequenced. it encodes a polypeptide of 740 residues, which contains a signal sequence of 24 residues. sldh had 35-37% identity to the membrane-bound quinoprotein glucose dehydrogenases (gdhs) from e. coli, gluconobacter oxydans, and acinetobacter calcoaceticus except the n-terminal hydrophobic region of gdh. additionally, the sldb gene located just upstream of slda was found to en ... | 2002 | 11999397 |
| lack of microbiological concordance between bone and non-bone specimens in chronic osteomyelitis: an observational study. | prognosis of chronic osteomyelitis depends heavily on proper identification and treatment of the bone-infecting organism. current knowledge on selecting the best specimen for culture is confusing, and many consider that non-bone specimens are suitable to replace bone cultures. this paper compares the microbiology of non-bone specimens with bone cultures, taking the last as the diagnostic gold standard. | 2002 | 12015818 |
| comparative studies of the acinetobacter genus and the species identification method based on the reca sequences. | the reca gene is indispensable for a maintaining and diversification of the bacterial genetic material. given its important role in ensuring cell viability, it is not surprising that the reca protein is both ubiquitous and well conserved among a range of prokaryotes. previously, we reported acinetobacter genomic species identification method based on pcr amplification of an internal fragment of the reca gene with subsequent restriction analysis (rflp) with hinfi and mboi enzymes. in present stud ... | 2002 | 12005442 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| efficacy of teicoplanin and imipenem on respiratory infections in high risk patients. | the efficacy of teicoplanin and imipenem was assayed on microorganisms, mainly hospital opportunistic organisms, resistant to common therapy, isolated from bronchoaspirates of high risk hospitalized patients. imipenem has shown remarkable activity against all isolated microorganisms with sensitivities ranging from 91.93% against acinetobacter calcoaceticus to 75.86% against pseudomonas sp. teicoplanin has shown excellent activity against staphylococcus aureus (sensitivity 83.66%). | 1991 | 12041770 |
| a broad host range plasmid vector that does not encode replication proteins. | the 640-bp minimal replication region derived from a plasmid dna preparation from an acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, acinetobacter calcoaceticus and alcaligenes faecalis) was identified. this dna fragment (named tfk replicon) does not encode rep proteins and appears to be unrelated to other known replicons. | 2002 | 12052556 |
| bioengineered emulsans from acinetobacter calcoaceticusrag-1 transposon mutants. | transposon mutants of acinetobacter calcoaceticus strain rag-1 were studied in an effort to control fatty acid (fa) substitution patterns of emulsan, a bioemulsifier secreted by the organism. the disrupted genes, involved in the biosynthetic pathways of biotin, histidine, cysteine or purines, influenced the level and types of fas incorporated into emulsan. the structural variants of emulsan generated by the transposon mutants were characterized for yield, fa content, molecular weight, and emulsi ... | 2002 | 12111149 |
| bacteremia due to moraxella atlantae in a cancer patient. | a gram-negative alkaline phosphatase- and pyrrolidone peptidase-positive rod-shaped bacterium (ccug 45702) was isolated from two aerobic blood cultures from a female cancer patient. no identification could be reached using phenotypic techniques. amplification of the trna intergenic spacers revealed fragments with lengths of 116, 133, and 270 bp, but no such pattern was present in our reference library. sequencing of the 16s rrna gene revealed its identity as moraxella atlantae, a species isolate ... | 2002 | 12089312 |
| gene discovery within the planctomycete division of the domain bacteria using sequence tags from genomic dna libraries. | the planctomycetes comprise a distinct group of the domain bacteria, forming a separate division by phylogenetic analysis. the organization of their cells into membrane-defined compartments including membrane-bounded nucleoids, their budding reproduction and complete absence of peptidoglycan distinguish them from most other bacteria. a random sequencing approach was applied to the genomes of two planctomycete species, gemmata obscuriglobus and pirellula marina, to discover genes relevant to thei ... | 2002 | 12093378 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| vibrio cholerae cytr is a repressor of biofilm development. | vibrio cholerae is both a human pathogen and a natural inhabitant of aquatic environments. in the aquatic environment, microorganisms are found attached to surfaces in structures known as biofilms. we have identified a transcriptional repressor in v. cholerae that inhibits exopolysaccharide synthesis and biofilm development. our studies show that this repressor is the v. cholerae homologue of escherichia coli cytr, a protein that represses nucleoside uptake and catabolism when nucleosides are sc ... | 2002 | 12123457 |
| regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation. | the riboflavin biosynthesis in bacteria was analyzed using comparative analysis of genes, operons and regulatory elements. a model for regulation based on formation of alternative rna structures involving the rfn elements is suggested. in gram-positive bacteria including actinomycetes, thermotoga, thermus and deinococcus, the riboflavin metabolism and transport genes are predicted to be regulated by transcriptional attenuation, whereas in most gram-negative bacteria, the riboflavin biosynthesis ... | 2002 | 12136096 |
| roles for the two 1-butanol dehydrogenases of pseudomonas butanovora in butane and 1-butanol metabolism. | pseudomonas butanovora grown on butane or 1-butanol expresses two 1-butanol dehydrogenases, a quinoprotein (boh) and a quinohemoprotein (bdh). boh exhibited high affinity towards 1-butanol (k(m) = 1.7 +/- 0.2 microm). boh also oxidized butyraldehyde and 2-butanol (k(m) = 369 +/- 85 microm and k(m) = 662 +/- 98 microm, respectively). the mrna induction profiles of boh and bdh at three different levels of 1-butanol, a nontoxic level (0.1 mm), a growth-supporting level (2 mm), and a toxic level (40 ... | 2002 | 12142403 |
| identification of bacteria in drinking and purified water during the monitoring of a typical water purification system. | a typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments. | 2002 | 12182763 |
| in vitro and in vivo antibacterial activities of dw286, a new fluoronaphthyridone antibiotic. | the in vitro and in vivo activities of dw286, a novel fluoronaphthyridone with potent antibacterial activity, were compared with those of ciprofloxacin, gemifloxacin, sparfloxacin, and trovafloxacin. against gram-positive bacteria, such as staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis, the in vitro activity of dw286 was stronger than that of any other reference antibiotic. against gram-negative bacteria, the activity of dw286 was similar t ... | 2002 | 12183275 |
| theriogenology question of the month. treatment options for erosive seminal vesiculitis caused by acinetobacter calcoaceticus. | | 2002 | 12322915 |
| rhamnolipid stimulates uptake of hydrophobic compounds by pseudomonas aeruginosa. | the biodegradation of hexadecane by five biosurfactant-producing bacterial strains (pseudomonas aeruginosa ug2, acinetobacter calcoaceticus rag1, rhodococcus erythropolis dsm 43066, r. erythropolis atcc 19558, and strain bcg112) was determined in the presence and absence of exogenously added biosurfactants. the degradation of hexadecane by p. aeruginosa was stimulated only by the rhamnolipid biosurfactant produced by the same organism. this rhamnolipid did not stimulate the biodegradation of hex ... | 2002 | 12200306 |
| conservation of the biotin regulon and the bira regulatory signal in eubacteria and archaea. | biotin is a necessary cofactor of numerous biotin-dependent carboxylases in a variety of microorganisms. the strict control of biotin biosynthesis in escherichia coli is mediated by the bifunctional bira protein, which acts both as a biotin-protein ligase and as a transcriptional repressor of the biotin operon. little is known about regulation of biotin biosynthesis in other bacteria. using comparative genomics and phylogenetic analysis, we describe the biotin biosynthetic pathway and the bira r ... | 2002 | 12368242 |
| reactor operation and scale-up of whole cell baeyer-villiger catalyzed lactone synthesis. | the recombinant whole cell biocatalyst escherichia coli top10 [pqr239], expressing cyclohexanone monooxygenase from acinetobacter calcoaceticus ncimb 9871, was used in 1.5- and 55-l fed-batch processes to oxidize bicyclo[3.2.0]hept-2-en-6-one to its corresponding regioisomeric lactones, (-)-(1s,5r)-2-oxabicyclo[3.3.0]oct-6-en-3-one and (-)-(1r,5s)-3-oxabicyclo[3.3.0]oct-6-en-2-one. by employing a bicyclo[3.2.0]hept-2-en-6-one feed rate below that of the theoretical volumetric biocatalyst activit ... | 2002 | 12363355 |
| genes coding for a new pathway of aerobic benzoate metabolism in azoarcus evansii. | a new pathway for aerobic benzoate oxidation has been postulated for azoarcus evansii and for a bacillus stearothermophilus-like strain. benzoate is first transformed into benzoyl coenzyme a (benzoyl-coa), which subsequently is oxidized to 3-hydroxyadipyl-coa and then to 3-ketoadipyl-coa; all intermediates are coa thioesters. the genes coding for this benzoate-induced pathway were investigated in the beta-proteobacterium a. evansii. they were identified on the basis of n-terminal amino acid sequ ... | 2002 | 12399500 |
| failure to demonstrate involvement of antibodies to acinetobacter calcoaceticus in transmissible spongiform encephalopathies of animals. | acinetobacter calcoaceticus, a soil microbe, contains molecular sequences which resemble those found in neurofilaments of the brain tissue. it was hypothesized that if cattle ingest large amounts of feedstuff containing a. calcoaceticus, they may develop an autoimmune reaction, with consequences of pathological changes associated with transmissible spongiform encephalopathies (tses). the hypothesis was tested using a small number of serum samples collected from cattle and it was found that affec ... | 2002 | 12383651 |
| adjuvant activity of emulsan, a secreted lipopolysaccharide from acinetobacter calcoaceticus. | several promising adjuvant candidates have been studied over the past 75 years; however, only alum is currently approved for human use. the complex acylated polysaccharide emulsan, secreted from acinetobacter calcoaceticus, represents a new candidate. unique features of this family of polymers are their amenability to structural tailoring and their emulsification behavior. we demonstrate that emulsan activates macrophages in a dose-dependent manner. this activation is dependent on the presence o ... | 2002 | 12414756 |
| enzymatic preparation of d-beta-acetylthioisobutyric acid and cetraxate hydrochloride using a stereo- and/or regioselective hydrolase, 3,4-dihydrocoumarin hydrolase from acinetobacter calcoaceticus. | 3,4-dihydrocoumarin hydrolase (dch) from acinetobacter calcoaceticus f46, which was previously found on screening for aromatic lactone-hydrolyzing enzymes, catalyzes the hydrolysis of several linear esters. the substrate specificity of the enzyme toward linear esters was quite characteristic, i.e., (1) it was specific toward methyl esters, (2) it recognized the configuration at the 2-position, and (3) it hydrolyzed diesters to monoesters. dch hydrolyzed the methyl esters of beta-acetylthioisobut ... | 2002 | 12436309 |
| a polyphosphate kinase (ppk2) widely conserved in bacteria. | synthesis of inorganic polyphosphate (poly p) from the terminal phosphate of atp is catalyzed reversibly by poly p kinase (ppk, now designated ppk1) initially isolated from escherichia coli. ppk1 is highly conserved in many bacteria, including some of the major pathogens such as pseudomonas aeruginosa. in a null mutant of p. aeruginosa lacking ppk1, we have discovered a previously uncharacterized ppk activity (designated ppk2) distinguished from ppk1 by the following: synthesis of poly p from gt ... | 2002 | 12486232 |
| genetic and phenotypic analysis of acinetobacter baumannii insertion derivatives generated with a transposome system. | acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. however, little is known about the genes and factors involved in its basic physiology and virulence properties. insertion mutagenesis was used to initiate the identification and characterization of some of these factors and genes in the prototype strain 19606. the utilization of the plofkm suicide delivery vector, which harbors a suicide mini-tn10 derivative, proved to be unsucces ... | 2002 | 12450860 |
| comparison of a repetitive extragenic palindromic sequence-based pcr method and clinical and microbiological methods for determining strain sources in cases of nosocomial acinetobacter baumannii bacteremia. | using a repetitive extragenic palindromic pcr (rep-pcr), we genotypically characterized strains causing nosocomial acinetobacter baumannii infections and analyzed the source of bacteremia in 67 patients from an institution in which infections by this bacterium were endemic. six different genotypes were found, including 21, 27, 3, 9, 3, and 4 strains. the probable source of bacteremia, according to clinical and/or microbiological criteria, was known in 42 patients (63%): respiratory tract (n = 19 ... | 2002 | 12454154 |
| precipitin response of potato processing workers to work-related microbial allergens. | serum samples from 61 potato processing workers and 30 urban dwellers not exposed to organic dusts (as a reference group) were examined in agar-gel precipitation test performed by ouchterlony double diffusion method with the antigens of 12 microorganisms associated with organic dusts. each serum was tested twice: not concentrated, and three-fold concentrated, for the detection of low levels of precipitins. the antibody response of workers to the antigen of coryneform bacterium agromyces ramosus ... | 2002 | 12498593 |
| lipase activity and gene cloning of acinetobacter calcoaceticus lp009. | the production of lipase from acinetobacter calcoaceticus lp009, a bacterium isolated from raw milk, was found to be best induced by tween-80 at 1.0% concentration. it was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. a. calcoaceticus lp009 lipase exhibited maximum activity at ph 7.0 and 50 degrees c, and was relatively stable upon storage at ph 5.0 to 7.0 and at 4, 30, or 37 degrees c. the enzyme was found to be inactivated by e ... | 1998 | 12501281 |
| comparison of the potential of coastal materials loaded with bacteria for bioremediating oily sea water in batch culture. | the objective of this paper was to study whether the bioremediation potential of coastal materials for oil-polluted sea water depended on the numbers of hydrocarbon-utilizing bacteria they naturally harbor. inshore water of the arabian gulf was found to contain only about one thousand hydrocarbon-utilizing bacteria per ml. coastal sand, cyanobacterial mats and epilithic biomass were much richer in these bacteria, with numbers ranging between several thousand fold to several million fold than in ... | 2002 | 12501998 |
| a novel bifunctional wax ester synthase/acyl-coa:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in acinetobacter calcoaceticus adp1. | triacylglycerols (tags) and wax esters are neutral lipids with considerable importance for dietetic, technical, cosmetic, and pharmaceutical applications. acinetobacter calcoaceticus adp1 accumulates wax esters and tags as intracellular storage lipids. we describe here the identification of a bifunctional enzyme from this bacterium exhibiting acyl-coa:fatty alcohol acyltransferase (wax ester synthase, ws) as well as acyl-coa:diacylglycerol acyltransferase (dgat) activity. experiments with a knoc ... | 2003 | 12502715 |
| degradation and transformability of dna from transgenic leaves. | the fate of transplastomic (chloroplast genome contains the transgene) tobacco plant dna in planta was studied when the plant leaves were subjected to decay conditions simulating those encountered naturally, including grinding, incubation with cellulase or enzymes produced by erwinia chrysanthemi, and attack by the plant pathogen ralstonia solanacearum. direct visualization of dna on agarose gels, gene extraction yield (the number of amplifiable aada sequences in extracted plant dna), and the fr ... | 2003 | 12514059 |
| an outer membrane enzyme that generates the 2-amino-2-deoxy-gluconate moiety of rhizobium leguminosarum lipid a. | the structures of rhizobium leguminosarum and rhizobium etli lipid a are distinct from those found in other gram-negative bacteria. whereas the more typical escherichia coli lipid a is a hexa-acylated disaccharide of glucosamine that is phosphorylated at positions 1 and 4', r. etli and r. leguminosarum lipid a consists of a mixture of structurally related species (designated a-e) that lack phosphate. a conserved distal unit, comprised of a diacylated glucosamine moiety with galacturonic acid res ... | 2003 | 12531907 |
| prediction and overview of the rpon-regulon in closely related species of the rhizobiales. | in the rhizobia, a group of symbiotic gram-negative soil bacteria, rpon (sigma54, sigman, ntra) is best known as the sigma factor enabling transcription of the nitrogen fixation genes. recent reports, however, demonstrate the involvement of rpon in other symbiotic functions, although no large-scale effort has yet been undertaken to unravel the rpon-regulon in rhizobia. we screened two complete rhizobial genomes (mesorhizobium loti, sinorhizobium meliloti) and four symbiotic regions (rhizobium et ... | 2002 | 12537565 |
| role of acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids. | the physiological role of a bifunctional enzyme, 3,4-dihydrocoumarin hydrolase (dch), which is capable of both hydrolysis of ester bonds and organic acid-assisted bromination of organic compounds, was investigated. purified dch from acinetobacter calcoaceticus f46 catalysed dose- and time-dependent degradation of peracetic acid. the gene (dch) was cloned from the chromosomal dna of the bacterium. the dch orf was 831 bp long, corresponding to a protein of 272 amino acid residues, and the deduced ... | 2003 | 12542698 |
| prevalence of plasmid-mediated quinolone resistance. | quinolone resistance encoded by the qnr gene and mediated by plasmid pmg252 was discovered in a clinical strain of klebsiella pneumoniae that was isolated in 1994 at the university of alabama at birmingham medical center. the gene codes for a protein that protects dna gyrase from quinolone inhibition and that belongs to the pentapeptide repeat family of proteins. the prevalence of the gene has been investigated by using pcr with qnr-specific primers with a sample of more than 350 gram-negative s ... | 2003 | 12543659 |
| [the detection of phenol degrading strain in environment with specific primer of phenol hydroxylase gene]. | a 684 bp oligonucleotide fragment was produced by pcr amplification from phenol-degrading strain acinetobacter calcoaceticus phea-2 with the specific primers of gene encoding phenol hydroxylase. the nucleotide sequence of this fragment and its deduced amino acid sequence share 84% and 98% homology with the phenol hydroxylase gene and its deduced amino acid sequences of phenol-degrading strain acinetobacter calcoaceticus ncib8250. sets of different aromatic compounds degrading strains were used t ... | 2001 | 12549083 |
| cloning of acinetobacter calcoaceticus chromosomal region involved in catechin degradation. | acinetobacter calcoaceticus utilizes catechin as sole carbon source. the chromosomal region involved in catechin catabolism was cloned in escherichia coli dh5alpha from the genomic dna of a. calcoaceticus. a recombinant e. coli containing 9.2 kb dna fragment of a. calcoaceticus inserted in puc19 showed a halo zone around the colony in plate assays, indicating the catechin utilizing ability of the clone. enzyme assays revealed the expression of the cloned dna fragment of a. calcoaceticus. high pe ... | 2003 | 12608578 |
| synthesis of imidazol-2-yl amino acids by using cells from alkane-oxidizing bacteria. | sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize n-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. after growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). high yields of imidazol-2-yl amino acids were produced by the strains gordonia rubropertincta sbug 105, gordonia terra ... | 2003 | 12620858 |
| comparison of methods for dna isolation from food samples for detection of shiga toxin-producing escherichia coli by real-time pcr. | in this study, food samples were intentionally contaminated with escherichia coli o157:h7, and then dna was isolated by using four commercial kits. the isolated dna samples were compared by using real-time pcr detection of the shiga toxin genes. the four kits tested worked similarly. | 2003 | 12620880 |
| bacteremia caused by acinetobacter ursingii. | acinetobacter ursingii has not been reported in infectious processes apart from its recent description as a new species. a bacteremia caused by a. ursingii in a patient with a pulmonary adenocarcinoma confirms that this microorganism is an opportunistic human pathogen. the isolate was susceptible to imipenem, aminoglycosides, rifampin, and fluoroquinolones. | 2003 | 12624081 |
| isolation of antibiotic hypersusceptibility mutants of acinetobacter spp. by selection for dna release. | isolation of bacterial mutants hypersusceptible to antibiotics can reveal novel targets for antibiotic potentiators. however, identification of such mutants is a difficult task which normally requires laborious replica plating of thousands of colonies. the technique proposed here allows for the positive selection of genetic knockout mutants leading to hypersusceptibility. this technique, designated sdr (selection for dna release), involves introduction of random insertions of a marker gene into ... | 2003 | 12654657 |
| epidemiology of rifampin adp-ribosyltransferase (arr-2) and metallo-beta-lactamase (blaimp-4) gene cassettes in class 1 integrons in acinetobacter strains isolated from blood cultures in 1997 to 2000. | we characterized two new gene cassettes in an acinetobacter isolate: one harbored the metallo-beta-lactamase (imp-4) gene bla(imp-4), the other harbored the rifampin adp-ribosyltransferase (arr-2) gene arr-2, and both arrayed with the aminoglycoside acetyltransferase [aac(6')-ib(7)] gene cassette aaca4 in two separate class 1 integrons. the epidemiology of these gene cassettes in isolates from blood cultures obtained from 1997 to 2000 was studied. isolates bearing either the bla(imp-4) or the ar ... | 2003 | 12654674 |
| significance of genomic dna group delineation in comparative studies of antimicrobial susceptibility of acinetobacter spp. | there were significant differences in antimicrobial susceptibilities in isolates of genomic dna groups 2 (acinetobacter baumannii), 3, and 13tu collected from the same sources, e.g., patients in intensive care units and general wards, and in isolates of the same group collected from different sources. the delineation of genomic groups is important in comparative surveillance studies of antimicrobial susceptibilities. | 2003 | 12654697 |
| transcriptional cross-regulation of the catechol and protocatechuate branches of the beta-ketoadipate pathway contributes to carbon source-dependent expression of the acinetobacter sp. strain adp1 poba gene. | transcriptional control of carbon source preferences by acinetobacter sp. strain adp1 was assessed with a poba::lacz fusion during growth on alternative substrates. the poba-encoded enzyme catalyzes the first step in the degradation of 4-hydroxybenzoate, a compound consumed rapidly as a sole carbon source. if additional aromatic carbon sources are available, 4-hydroxybenzoate consumption is inhibited by unknown mechanisms. as reported here, during growth on aromatic substrates, poba was not expr ... | 2003 | 12620848 |
| the improvement of lipase secretion and stability by addition of inert compounds into acinetobacter calcoaceticus cultures. | acinetobacter calcoaceticus bd413 produces variable amounts of an exocellular lipase that becomes rapidly inactivated upon secretion. to achieve high yield and protect the enzyme, we assayed the addition of several inert compounds to cell-free supernatants, cell fractions, and whole cultures. glass beads, poly(ethylene glycol) 600, triton x-100, saccharose, gum arabic, and beta-cyclodextrin were among the compounds tested. beta-cyclodextrin and gum arabic (and saccharose to a lesser extent) were ... | 2002 | 12619817 |
| isolation and characterization of thermophilic bacilli degrading cinnamic, 4-coumaric, and ferulic acids. | thirty-four thermophilic bacillus sp. strains were isolated from decayed wood bark and a hot spring water sample based on their ability to degrade vanillic acid under thermophilic conditions. it was found that these bacteria were able to degrade a wide range of aromatic acids such as cinnamic, 4-coumaric, 3-phenylpropionic, 3-(p-hydroxyphenyl)propionic, ferulic, benzoic, and 4-hydroxybenzoic acids. the metabolic pathways for the degradation of these aromatic acids at 60 degrees c were examined b ... | 2003 | 12620824 |
| effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in acinetobacter calcoaceticus. | the adaptive responses of the bacterium acinetobacter calcoaceticus to different aliphatic alcohols on the level of the membrane fatty acids were studied in detail. the toxicity of the aliphatic alcohols increased with an increasing hydrophobicity. as alcohols are known to increase the fluidity of the membrane they consequently should cause the same adaptive effect on membrane level. yet, cells of a. calcoaceticus react completely different to the alcohols: in the presence of long-chained alcoho ... | 2003 | 12670684 |
| thyroid abscess due to acinetobacter calcoaceticus: case report and review of the causes of and current management strategies for thyroid abscesses. | thyroid abscess was a common condition in the era before antibiotics. in the current medical environment, however, it is a clinical entity that is seldom encountered. we report the case of a unique cause of thyroid abscess, the environmental gram-negative bacterium acinetobacter calcoaceticus. review of the published causes of thyroid abscess since 1980 demonstrated that although gram-positive bacteria (staphylococcus and streptococcus species) remain the most common causes, there has been a mar ... | 2003 | 12659365 |
| new quinoproteins in oxidative fermentation. | several quinoproteins have been newly indicated in acetic acid bacteria, all of which can be applied to fermentative or enzymatic production of useful materials by means of oxidative fermentation. (1) d-arabitol dehydrogenase from gluconobacter suboxydans ifo 3257 was purified from the bacterial membrane and found to be a versatile enzyme for oxidation of various substrates to the corresponding oxidation products. it is worthy of notice that the enzyme catalyzes d-gluconate oxidation to 5-keto-d ... | 2003 | 12686101 |
| glucose enzyme electrode using cytochrome b(562) as an electron mediator. | we demonstrate the construction of glucose sensors employing pyrroloquinoline quinone (pqq) glucose dehydrogenase (pqqgdh) from acinetobacter calcoaceticus and glucose oxidase (god) from aspergillus nigar coupled with escherichia coli soluble cytochrome b(562) (cyt b(562)) as electron acceptor. pqqgdh and god do not show direct electrochemical recycling of the prosthetic group at the electrode surface leading to a corresponding current signal. we constructed pqqgdh and god electrodes co-immobili ... | 2003 | 12706581 |
| membrane-bound d-sorbitol dehydrogenase of gluconobacter suboxydans ifo 3255--enzymatic and genetic characterization. | gluconobacter strains effectively produce l-sorbose from d-sorbitol because of strong activity of the d-sorbitol dehydrogenase (sldh). l-sorbose is one of the important intermediates in the industrial vitamin c production process. two kinds of membrane-bound sldhs, which consist of three subunits, were reportedly found in gluconobacter strains [agric. biol. chem. 46 (1982) 135,fems microbiol. lett. 125 (1995) 45]. we purified a one-subunit-type sldh (80 kda) from the membrane fraction of glucono ... | 2003 | 12686146 |
| burkholderia is highly resistant to human beta-defensin 3. | the bactericidal activity of the novel beta-defensin hbd-3 against 28 species and 55 strains of gram-positive cocci and gram-negative fermentative and nonfermentative rods was tested. all strains proved to be highly or intermediately susceptible to hbd-3 (minimal bactericidal concentration [mbc], </=50 micro g/ml), except for burkholderia cepacia, for all 23 tested strains of which mbcs were >100 micro g/ml. | 2003 | 12709350 |
| an exocellular protein from the oil-degrading microbe acinetobacter venetianus rag-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. | the oil-degrading microorganism acinetobacter venetianus rag-1 produces an extracellular polyanionic, heteropolysaccharide bioemulsifier termed emulsan. emulsan forms and stabilizes oil-water emulsions with a variety of hydrophobic substrates. removal of the protein fraction yields a product, apoemulsan, which exhibits much lower emulsifying activity on hydrophobic substrates such as n-hexadecane. one of the key proteins associated with the emulsan complex is a cell surface esterase. the esteras ... | 2003 | 12732528 |
| genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in acinetobacter calcoaceticus phea-2. | acinetobacter calcoaceticus phea-2 is a phenol-degrading bacterium isolated from the wastewater from an oil refinery. a 10-kb xhoi fragment consisting of nine complete open reading frames (orfs) and one partial orf was screened from a lambda library of phea-2 by southern hybridization. the sequence analyses revealed that orf2-orf7, designated mphklmnop, are homologous to dmpklmnop of pseudomonas sp. cf600 and mopklmnop of acinetobacter calcoaceticus ncib8250, sharing 38%-72% and 58.5%-93.5% resp ... | 2003 | 12732969 |
| gene cloning, sequencing, and expression of an esterase from acinetobacter lwoffii i6c-1. | the esterase-encoding gene, esta, was cloned from acinetobacter lwoffii i6c-1 genomic dna into escherichia coli bl21(de3) with plasmid vector pet-22b (pem1). pem1 has a 4.4-kb ecori insert that contained the complete esta gene. a 2.4-kb avai- sphi dna fragment was subcloned (pem3) and sequenced. esta gene encodes a protein of 366 amino acids (40,687 da) with a pi of 9.17. the esta signal peptide was 31 amino acids long, and the mature esterase sequence is 335 amino acids long (37.5 kda). the con ... | 2003 | 12732980 |
| degradation of (+)-catechin by acinetobacter calcoaceticus mtc 127. | acinetobacter calcoaceticus mtc 127 was able to grow on catechin and protocatechuic acid (pca) as sole carbon source. cells induced with catechin oxidized catechin and pca at rates higher than cells of uninduced cultures. two aromatic compounds, pca and phloroglucinol carboxylic acid (pgca) were isolated from culture filtrate of cells grown in catechin and characterized by infrared spectrometry and high performance thin-layer chromatography. moreover, a. calcoaceticus mtc 127 produced high level ... | 2003 | 12787923 |
| efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of hansenula polymorpha. | a high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. an efficient library construction method was developed by using in vivo recombination in hansenula polymorpha. various linear sets of vectors and insert fragments were transformed and analyzed to optimize the in vivo recombination system. a telomere-originated autonomously replicating sequence (ars) of h. p ... | 2003 | 12902228 |
| dual labeling of pseudomonas putida with fluorescent proteins for in situ monitoring of conjugal transfer of the tol plasmid. | we describe here a dual-labeling technique involving the green fluorescent protein (gfp) and the red fluorescent protein (dsred) for in situ monitoring of horizontal gene transfer via conjugation. a gfpmut3b-tagged derivative of narrow-host-range tol plasmid (pwwo) was delivered to pseudomonas putida kt2442, which was chromosomally labeled with dsred by transposon insertion via biparental mating. green and red fluorescent proteins were coexpressed in donor p. putida cells. cells expressing both ... | 2003 | 12902279 |
| growth of escherichia coli in model distribution system biofilms exposed to hypochlorous acid or monochloramine. | bacteria indigenous to water distribution systems were used to grow multispecies biofilms within continuous-flow slide chambers. six flow chambers were also inoculated with an escherichia coli isolate obtained from potable water. the effect of disinfectants on bacterial populations was determined after exposure of established biofilms to 1 ppm of hypochlorous acid (cloh) for 67 min or 4 ppm of monochloramine (nh(2)cl) for 155 min. to test the ability of bacterial populations to initiate biofilm ... | 2003 | 12957935 |
| semiquantitative detection by real-time pcr of aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis. | a real-time pcr method was developed and used to detect aspergillus fumigatus mitochondrial dna (mtdna) in bronchoalveolar lavage (bal) fluids and tissue biopsy specimens. the analytical sensitivity of the assay was one a. fumigatus conidium per reaction, and the assay was linear at least over 4 orders of magnitude above the detection limit. bal fluids from 66 immunocompromised patients at risk of invasive pulmonary aspergillosis (ipa) and 33 immunocompetent controls and tissue biopsy specimens ... | 2003 | 12958261 |
| characterization of hybrid toluate and benzoate dioxygenases. | toluate dioxygenase of pseudomonas putida mt-2 (tado(mt2)) and benzoate dioxygenase of acinetobacter calcoaceticus adp1 (bado(adp1)) catalyze the 1,2-dihydroxylation of different ranges of benzoates. the catalytic component of these enzymes is an oxygenase consisting of two subunits. to investigate the structural determinants of substrate specificity in these ring-hydroxylating dioxygenases, hybrid oxygenases consisting of the alpha subunit of one enzyme and the beta subunit of the other were pr ... | 2003 | 12949084 |
| cloning and characterization of the fur gene from moraxella bovis. | a homologue of the ferric uptake regulator gene (fur) was isolated from moraxella bovis by degenerate polymerase chain reaction and cloning. fur protein of m. bovis exhibited 72.1% amino acid identity with acinetobacter calcoaceticus fur. western blot analysis showed a decrease of fur expression in response to sufficient-iron conditions compared with deficient-iron conditions. an electrophoretic mobility-shift assay indicated that fur protein binds to dna fragments containing a putative fur-box ... | 2003 | 12906101 |
| surveillance of pediatric infections in a teaching hospital in mato grosso do sul, brazil. | nosocomial infections (ni) result in considerably high mortality and morbidity rates, especially among pediatric patients. considering current worldwide changes, information about the occurrence of pathogens and susceptibility tests are now seen as decisive for optimizing treatment. the purpose of this research was to determine the frequency of microorganisms, antimicrobial and genetic profiles, and risk factors associated with nosocomial infections in a teaching hospital in campo grande, mato g ... | 2003 | 12959687 |
| screening of bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens. | as probiotic bacteria, strains belonging to the genus bifidobacterium colonise the gastro-intestinal tract of humans and animals at the time of birth, and they are found in young as well as in adult individuals in great numbers. moreover, they can interact with the development of enteric infections by the production of antimicrobial metabolites. in this work 281 strains of bifidobacteria were anaerobically isolated from human faecal samples, supplied by volunteers of different ages (youngs, adul ... | 2003 | 12906392 |
| prevalence and in-vitro antimicrobial susceptibility patterns of acinetobacter strains isolated from patients in intensive care units. | fifty-six acinetobacter species strains (49 acinetobacter baumanii, 5 acinetobacter calcoaceticus, 2 acinetobacter iwoffii) were detected using both conventional methods and gas chromatography of bacterial fatty acids with the midi sherlock microbial identification system. the susceptibilities of these strains to 16 antimicrobial agents were investigated by the disc-diffusion method according to the national committee for clinical laboratory standards. the production of extended-spectrum beta-la ... | 2003 | 12964502 |
| [degradation metabolism of 2-3 butanediol and of acetoin by microorganisms; considerations on neisseria winogradskyi. i. investigations on 2-3 butanediol dehydrogenase]. | | 1953 | 13124973 |
| [utilization in competition of sodium beta-hydroxybutyrate by neisseria winogradskyi]. | | 1952 | 13017136 |
| real-time pcr assay targets the 23s-5s spacer for direct detection and differentiation of legionella spp. and legionella pneumophila. | a real-time pcr for the abi prism 7000 system targeting the 23s-5s spacer of legionella spp. was developed. simultaneous detection and differentiation of legionella spp. and legionella pneumophila within 90 min and without post-pcr melting-curve analysis was achieved using two taqman probes. in sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed. | 2003 | 14532229 |
| [pathogenic or saprophytic bacteria transforming glucose to gluconic acid, bacterium anitratum, b5w, moraxella lwoffi var. glucidolytica, neisseria winogradskyi]. | | 1954 | 13171653 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| molecular characterization of the acute inflammatory response to infections with gram-negative versus gram-positive bacteria. | sepsis caused by gram-negative bacteria and that caused by gram-positive bacteria often manifest similar clinical features. we investigated plasma proinflammatory cytokine profiles in patients with sepsis due to gram-positive and gram-negative bacteria and studied the cytokine production and differential gene regulation of leukocytes stimulated ex vivo with escherichia coli lipopolysaccharide or heat-killed staphylococcus aureus. concentrations of tumor necrosis factor alpha, interleukin 1 recep ... | 2003 | 14500502 |
| purification and characterization of membrane-bound quinoprotein quinate dehydrogenase. | several bacterial strains carrying quinoprotein quinate dehydrogenase (qdh) were screened through acetic acid bacteria and other bacteria. strong enzyme activity was found in the membrane fraction of gluconobacter melanogenus ifo 3294, g. oxydans ifo 3292, g. oxydans ifo 3244, and some strains of acinetobacter calcoaceticus. interestingly, in the membrane fraction of a. calcoaceticus ac3, which is unable to produce pyrroloquinoline quinone (pqq), fairly large amounts of apo-qdh were formed, and ... | 2003 | 14586098 |
| prediction of reduction potential changes in rubredoxin: a molecular mechanics approach. | predicting the effects of mutation on the reduction potential of proteins is crucial in understanding how reduction potentials are modulated by the protein environment. previously, we proposed that an alanine vs. a valine at residue 44 leads to a 50-mv difference in reduction potential found in homologous rubredoxins because of a shift in the polar backbone relative to the iron site due to the different side-chain sizes. here, the aim is to determine the effects of mutations to glycine, isoleuci ... | 2003 | 14581187 |
| temporal changes in the bacterioplankton of a northeast ohio (usa) river. | to examine temporal changes in a bacterial community, water samples were collected monthly for one year from five sites along a major use-reuse river, the cuyahoga river, in northeastern ohio (usa). fluorescent in situ hybridization (fish) was used to enumerate population sizes of two species of common bacteria, pseudomonas putida and acinetobacter calcoaceticus; fish was also performed with a domain bacteria specific probe. in addition, the total bacteria (based on dapi staining), colony formin ... | 2002 | 14552350 |
| determination of decimal reduction time (d value) of chemical agents used in hospitals for disinfection purposes. | prior to the selection of disinfectants for low, intermediate and high (sterilizing) levels, the decimal reduction time, d-value, for the most common and persistent bacteria identified at a health care facility should be determined. | 2003 | 14563217 |
| high-resolution differentiation of cyanobacteria by using rrna-internal transcribed spacer denaturing gradient gel electrophoresis. | for many ecological studies of cyanobacteria, it is essential that closely related species or strains can be discriminated. since this is often not possible by using morphological features, cyanobacteria are frequently studied by using dna-based methods. a powerful method for analysis of the diversity and dynamics of microbial populations and for checking the purity and affiliation of cultivated strains is denaturing gradient gel electrophoresis (dgge). we realized high-resolution discrimination ... | 2003 | 14602623 |
| analysis of bacterial dna patterns--an approach for controlling biotechnological processes. | optimisation of biotechnological processes catalysed by microbial cells requires detailed information about operational limits of the single cells. their performance is correlated with distinct physiological states. we related these states to cell cycle events, which were found to proceed extremely diversely in different bacterial strains. characteristic dna patterns were found flow cytometrically, depending on the type of strain, substrates and growth conditions involved; this information can b ... | 2003 | 14607431 |
| new bacterial pathway for 4- and 5-chlorosalicylate degradation via 4-chlorocatechol and maleylacetate in pseudomonas sp. strain mt1. | pseudomonas sp. strain mt1 is capable of degrading 4- and 5-chlorosalicylates via 4-chlorocatechol, 3-chloromuconate, and maleylacetate by a novel pathway. 3-chloromuconate is transformed by muconate cycloisomerase of mt1 into protoanemonin, a dominant reaction product, as previously shown for other muconate cycloisomerases. however, kinetic data indicate that the muconate cycloisomerase of mt1 is specialized for 3-chloromuconate conversion and is not able to form cis-dienelactone. protoanemonin ... | 2003 | 14617643 |