| classification of acute bacterial meningitis cases according to their etiology, seen in a children's hospital in ankara from march 1973 to april 1974. | in a 14 months period, as from march 1973 to april 1974 468 cases of acute bacterial meningitis have been diagnosed by c.s.f. findings; namely by examining the protein and sugar content, by the cell counts, gram's staining and culturing of the sediments of c.s.f. 188 cases were proven by culturing to be meningococcic meningitis. all these cases are from the families living in suburban areas of the city under crowded circumstances, which show the importance of population density in the epidemiolo ... | 1976 | 6862 |
| various peculiarities of acinetobacter anitratum k-9 growth on media with ethanol. | | 1976 | 12454 |
| studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase. | antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ... | 1978 | 31146 |
| precipitate produced by serratia marcescens on macconkey agar: useful diagnostic test. | the production of a precipitate by serratia marcescens on oxoid macconkey agar has proven useful as a laboratory diagnostic test. this phenomenon is specific for serratia within the enterobacteriaceae, although precipitate production is also given by acinetobacter anitratus and some pseudomonas, alcaligenes, and aeromonas species. precipitate production seems to be specific for certain batches of macconkey agar, and is probably related to a specific property of some batches of bile salts. | 1978 | 32188 |
| biosynthesis of ethylene from methionine. isolation of the putative intermediate 4-methylthio-2-oxobutanoate from culture fluids of bacteria and fungi. | methods are described for identifying the 2,4-dinitrophenylhydrazones of 4-methylthio-2-oxobutanoate by means of t.l.c., n.m.r. and mass spectroscopy. by using these methods 4-methylthio-2-oxobutanoate, a putative intermediate in the biosynthesis of ethylene from methionine, has been identified in culture fluids of aeromonas hydrophila b12e and a coryneform bacterium d7f grown in the presence of methionine. relative to 4-methylthio-2-oxobutanoate, the yield of 3-(methylthio)propanal (methional) ... | 1979 | 42392 |
| the ribosomal serine proteinase: cathepsin r. | as has been known for several years, thoroughly purified ribosomes contain a firmly bound serine proteinase with an optimum of activity at neutral ph. the present paper shows that the activity is found in free cytoplasmic ribosomes as well as in ribosomes detached from the membranes of the endoplasmic reticulum of rat liver. after ribosome dissociation, the proteinase activity is found only on the 40 s subunits. recovery of the proteinase in the proteins of whole ribosomes or of 40 s subunits am ... | 1979 | 45162 |
| fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria. | the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ... | 1975 | 46774 |
| catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties. | procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ... | 1976 | 58860 |
| amikacin, an aminoglycoside with marked activity against antibiotic-resistant clinical isolates. | a total of 319 clinical isolates known to be resistant to one or more aminoglycoside antibiotics were tested for their susceptibility to 10 aminoglycosides. the percentages of isolates found by an agar dilution method to be susceptible were: amikacin, 83.7%; tobramycin, 41.4%; butirosin a, 33.2%; dideoxykanamycin b, 32.6%; gentamicin c, 27.3%; lividomycin a, 17.6%; neomycin b, 10.7%; paromomycin, 10.3%; kanamycin a, 10.0%; and ribostamycin, 7.2%. the effectiveness of the antibiotics was related ... | 1976 | 62814 |
| synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella. | antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ... | 1978 | 78659 |
| [infection by glucose-nonfermenting, gram-negative rods--infection by acinetobacter calcoaceticus]. | | 1977 | 101606 |
| homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria. | the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ... | 1979 | 104972 |
| sources of psychrotrophic bacteria on meat at the abattoir. | | 1978 | 100487 |
| studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. | | 1979 | 94750 |
| in-vitro sensitivity to mecillinam of multiresistant gram-negative rods. | | 1978 | 74642 |
| [drug sensitivity of glucose-nonfermenting bacteria other than pseudomonas aeruginosa (author's transl)]. | | 1979 | 119072 |
| community-acquired acinetobacter calcoaceticus var anitratus pneumonia. | two patients had community-acquired acinetobacter calcoaceticus var anitratus pneumonia. both patients were alcoholic and one was cirrhotic. one patient died and the other received two weeks of gentamicin therapy and survived. misinterpretation of the sputum gram stain delayed diagnosis and institution of proper therapy in both cases. in addition to organisms sensitive to penicillins such as neisseria or haemophilus, acinetobacter must be considered in the differential diagnosis of community-acq ... | 1977 | 70540 |
| piperacillin, a new penicillin active against many bacteria resistant to other penicillins. | the in vitro activity of piperacillin, a new semisynthetic piperazine penicillin derivative, was evaluated against 626 clinical isolates and compared with the activity of other beta-lactam antibiotics. at a concentration of 0.1 microgram/ml, piperacillin inhibited all streptococci except enterococci. non-beta-lactamase-producing staphylococci were inhibited by 1.6 microgram or less per ml. both beta-lactamase- and non-beta-lactamase-producing haemophilus were inhibited by 0.1 microgram/ml. piper ... | 1978 | 122519 |
| a gram stain for smears of blood cultures, body fluids and tissues. | a new gram stain method for blood cultures was adapted from a method for tissue sections. this was accomplished by comparing various modifications of the latter method on positive smears prepared from a 24-hour negative blood culture seeded with staphylococcus aureus and escherichia coli. a blind study was then conducted using the new method and hucker's method on 24-hour and 72-hour blood cultures as well as a limited number of other body fluids and tissues. of the 35 positive blood cultures de ... | 1976 | 62513 |
| [properties of rubredoxin reductase from the alkane-assimilating bacterium acinetobacter calcoaceticus]. | | 1979 | 44771 |
| differential toxicities of mercury to bacteria and bacteriophages in sea and in lake water. | mixtures of anionic hgcl3-/hgcl4(2)-complexes were less toxic to terrestrial bacteria (erwinia herbicola, agrobacterium tumefaciens), to marine bacteria (acinetobacter sp., aeromonas sp.), and to bacteriophages (phi 11 m 15 of staphylococcus aureus and p1 of escherichia coli) than were equivalent concentrations of hg as cationic hg2+. the toxicity of 1 ppm hg to a. tumefaciens. aeromonas sp., and phi 11 m 15 was less in seawater than in lake water. inasmuch as the hg-cl species are formed in env ... | 1979 | 161510 |
| identification of nonfermentative gram-negative bacteria in the clinical laboratory. | a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ... | 1975 | 163060 |
| microbial assimilation of hydrocarbons. i. the fine-structure of a hydrocarbon oxidizing acinetobacter sp. | 1. the fine-structure analysis of the hydrocarbon oxidizing microorganism, acinetobacter sp., demonstrated a cytoplasmic modification resulting from growth on paraffinic and olefinic hydrocarbons. 2. intracytoplasmic hydrocarbon inclusions were documented by electron microscopy with chemical identifications obtained by gas chromatography and x-ray diffraction. 3. these results demonstrate the ability of a microorganism to accumulate hydrocarbon substrates intracellularly which, in turn, indicate ... | 1975 | 163624 |
| [purification and physico-chemical properties of collagenase synthesized by a bacterium of the type acinetobacter sp]. | the acinetobacter spec collagenase has been almost completely purified. this enzyme is a true collagenase the activity of which is high on collagen. the enzyme is active on insoluble collagen, gelatin and the synthetic pz-peptide, but has no proteolytic activity on casein or bovine serum-albumin. the collagenase was obtained on a simple medium with gelatin and yeast extract. the enzyme was purified by (nh4)2so4 precipitation. deae cellulose column chromatography, sephadex g 200 gel-filtration. t ... | 1979 | 43744 |
| [oxidation of homologous aliphatic aldehydes by membrane-bound and solubilized aldehyde dehydrogenase from acinetobacter calcoaceticus]. | | 1978 | 32669 |
| purification and properties of amylase produced by a moderately halophilic acinetobacter sp. | a moderately halophilic acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2m nacl or 1m kcl. two kinds of amylase, amylases i and ii were purified from the culture filtrate to an electrophoretically homogenous state by glycogen-complex formation, deae-sephadex a-50 chromatography, and sephadex g-200 gel filtration. both enzymes had maximal activi ... | 1978 | 30530 |
| the development of aerobic spoilage flora on meat stored at chill temperatures. | | 1977 | 22531 |
| purification and various properties of nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus. | the constitutive nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. the end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. the molecular weight of the enzyme was determined to be 235,000 by gel chromatography on sephadex g 200, the smallest subunit shows a molecular weight of 61 000 on sds electrophoresis. the isoelectric point is at 5.84. the km values determined with p ... | 1976 | 14464 |
| regulation of the malic enzyme activity of acinetobacter by organic acids. | | 1976 | 9741 |
| characteristics of a facultatively psychrophilic acinetobacter species isolated from river sediment. | a facultatively psychrophilic bacterium isolated from river sediment was identified as an acinetobacter species, similar to those previously characterized as a. lwoffi. the strain was extremely lipolytic and hemolytic. some action on crude oil was also observed. the organism was able to utilize a wide variety of carbon and energy sources when tested at both 20 and 30 degrees c. a comparison is made with the previously proposed type strain of a. lwoffi. the bacteria had a gram-negative cell wall ... | 1975 | 3275 |
| proceedings: bacterial degradation of salicylamide. | | 1975 | 2723 |
| control of nadp+-specific isocitrate dehydrogenase from acinetobacter by nucleotides. | | 1976 | 2501 |
| preparations and properties of ribonucleic acid polymerase from acinetobacter calcoaceticus. | deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) from acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the escherichia coli b enzyme. the molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. no subunit corresponding to that of sigma from e. coli was found, and furthermore no separation between the beta subunits could be detected by gel ... | 1976 | 1380 |
| the metabolism of cyclohexanol by acinetobacter ncib 9871. | acinetobacter ncib 9871 was isolated by elective culture on cyclohexanol and grows with this compound as sole source of carbon. it displays a restricted growth spectrum, being unable to grow on a wide range of alternative alicyclic alcohols and ketones. cyclohexanol-grown cells oxidize the growth substrate at a rate of 230 mul of o2/h per mg dry wt with the consumption of 5.65 mumol of o2/mumol substrate. cyclohexanone is oxidized at a similar rate with the consumption of 4.85 mumol of o2/mumol. ... | 1975 | 1261 |
| pyridine nucleotide-independent oxidation of long chain aliphatic alcohols by an enzyme of acinetobacter calcoaceticus. | | 1975 | 850 |
| properties of the nadp+-dependent isocitrate dehydrogenases of acinetobacter calcoaceticus. | | 1975 | 849 |
| [microbiological analysis of the results of treatment of urinary tract infections with epicillin]. | | 1979 | 112587 |
| [immunological characteristics of the protein antigens of the family neisseriaceae. ii. the importance of an immunochemical analysis of the protein complexes for a study of taxonomy problems]. | the study of antigenic interrelations in the family neisseriaceae resulted in the isolation of 2 main immunologically separated variants of protein complexes: the first variant was characteristic of nonpathogenic and pathogenic species of the genus neisseria as well as of 7 taxonomically undefined neisseria species (n. lactamicus, n. cuniculi, n. ellongata, n. ovis, n. animalis, n. cinerea, n. canis) and gemella haemolysans; the second variant was represented by the genera branhamella and acinet ... | 1979 | 116460 |
| microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes. | resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests. | 1978 | 100053 |
| stopcock: bacterial contamination in invasive monitoring systems. | | 1979 | 215570 |
| phosphatase activity of aerobic and facultative anaerobic bacteria. | 1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ... | 1978 | 216188 |
| effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls. | of 36 pure isomers (chlorine numbers 1 to 5) of polychlorinated biphenyls examined, 23 compounds were metabolized by alcaligenes sp. strain y42, and 33 compounds were metabolized by acinetobacter sp. strain p6. the major pathway of many polychlorinated biphenyl isomers examined was considered to proceed through 2',3'-dihydro-2',3'-diol compounds, concomitant dehydrogenated 2',3'-dihydroxy compounds, subsequently the 1',2'-meta-cleavage compounds (chlorinated derivatives of 2-hydroxy-6-oxo-6-phen ... | 1979 | 117752 |
| [taxonomic and classification position of bacteria of the genus bordetella]. | | 1979 | 220821 |
| sorption and desorption of atrazine by three bacterial species isolated from aquatic systems. | the isolates acinetobacter spec., cytophaga spec. and pseudomonas fluorescens represent different morphological and physiological types of bacteria. they accumulate atrazine (2-chloro-4-(ethylamino)-6-(isopropylamino)-5-triazine) to different levels. accumulation expressed by the ratio of atrazine sorbed per volume of bacteria to atrazine per the same volume of water amounted to 11 for acinetobacter spec., 8.6 for cytophaga spec. and 6.2 for pseudomonas fluorescens. accumulation ratios were prop ... | 1979 | 119497 |
| [distribution of ubiquinones (coenzyme q) in gram negative bacillae]. | the coenzyme q system was examined on 55 strains of gram negative aerobic or facultatively anaerobic rods. no bacteria contain co-q7 nor co-q10. ubiquinone q8 predominates in flavobacterium and in enterobacteriaceae; q9 was the only homolog found in the pseudomonas, and predominates in the acinetobacter. | 1975 | 126773 |
| [bacterial infection in cows associated with abortion, endometritis and sterility]. | isolated were two types of bacteria (having common morphologic and cultural behaviour and related antigenic composition) from aborted fetuses and placentae of cows with a positive reaction after both huddleson and placentae of cows with a positive reaction afterboth huddleson and wright. they were shown to be new species of the group mimeae and the genus yersinia. it was demonstrated through immunodiffusion and immunoelectrophoresis that these new bacteria present antigenic relationship with bru ... | 1976 | 65045 |
| isolation and characterization of membranes from a hydrocarbon-oxidizing acinetobacter sp. | membranes were isolated and purified from nutrient broth-yeast extract- and hexadecane-grown cells of acinetobacter sp. strain ho1-n. two membrane fractions were isolated from nutrient broth-yeast extract-grown cells, the cytoplasmic membrane and the outer membrane. in addition to these two membrane fractions, a unique membrane fraction was isolated from hexadecane-grown cells (band 1) and characterized as a lipid-rich, low-density membrane containing high concentrations of hexadecane. the outer ... | 1976 | 132429 |
| lipase and esterase formation by psychrophilic and mesophilic acinetobacter species. | acinetobacter o16, a psychrophilic species, produced extracellular lipase (measured by hydrolysis of olive oil, tributyrin, or beta-naphthyl laurate) when grown on a complex medium (peptone plus yeast extract). most lipase was produced during the logarithmic phase of growth. very little cell-bound lipase was formed. these cells also produced an esterase (measured by the hydrolysis of beta-naphthyl acetate). at first, all esterase was cell bound; significant amounts appeared in the external mediu ... | 1975 | 235353 |
| partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (acinetobacter o16). | the extracellular lipase(s) of the psychrophile acinetobacter o16 was studied. when the enzyme was precipitated by (nh4)2so4 and passed through a sephadex g200 column, two peaks of lipase activity appeared. the larger peak, which behaved like a substance of high molecular weight, being eluted in the void volume, was purified 250-fold over the crude enzyme (culture supernatant) by passage through a deae-sephadex column. when the enzyme was applied to a deae-cellulose column it could not be eluted ... | 1975 | 235354 |
| letter: urinary infections in general practice. | | 1976 | 58228 |
| the behaviour of nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation. | the behaviour of the nicotinamide adenine dinucleotides nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation was studied, acetate and succinate being used as reference carbon sources. the intracellular concentration of the two nucleotides was found to increase during the exponential growth phase, reaching its maximum in the phase of decreasing growth rates. in the exponential phase, the nad+/nadh quotients were less than 1 and showed only unimportant variations. in the phase ... | 1975 | 170172 |
| the nature of the attachment of a regularly arranged surface protein to the outer membrane of an acinetobacter sp. | acinetobacter 199a carries on the outer surface of its outer membrane a layer of regularly arranged protein subunits. the isolated surface protein assembles into the same regular array even in the absence of the underlying outer membrane. cl- minus is required for this self-assembly. evidence is presented that the interaction of the surface protein with the outer membrane involves the linking of a carboxyl group in the surface protein to a negatively charged group in the outer membrane protein, ... | 1975 | 237548 |
| the influence of changes in the environment on twitching motility. | by examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. incubation should take place in a humid atmosphere. in fact, everything points to the humidity as a factor of the utmost importance. using strains of acinetobacter calcoaceticus, it ... | 1975 | 239520 |
| influence of colloidal iron on the respiration of a species of the genus acinetobacter. | washed suspensions of acinetobacter sp. isolated from water caused the precipitation of iron from a suspension of colloidal ferric iron at ph 6.0 and 7.6. iron-encrusted cells of the bacterium formed large aggregates. the amount of iron removed from the colloidal preparation in the form of aggregates was from 21 to 52% at ph 7.6 and 49% an ph 6.0 by the bacterial cells. endogenous respiration rates of the iron-encrusted cells were from 32 to 72% lower than the rates for unencrusted cells. respir ... | 1975 | 239632 |
| bacterial respiration-linked proton translocation and its relationship to respiratory-chain composition. | 1. the relationship between chain composition and the efficiency of respiration-linked proton translocation was studied in nine bacterial species of widely differing taxonomic and ecological status. 2. all the bacteria investigated contained respiratory chain dehydrogenases, ubiquinone and/or menaquinone, cytochrome b and cytochrome oxidase aa3 and/or o. in addition, some of these organisms also contained pyridine nucleotide transhydrogenase and/or cytochrome c. 3. leads to h+/o ratios of whole ... | 1975 | 240679 |
| opportunistic infection in children. iii. in the normal host. | | 1975 | 171360 |
| [antimicrobial susceptibility patterns of clinical isolates of pseudomonas cepacia (author's transl)]. | the yearly changes of relative frequency of glucose-nonfermentative gram-negative bacilli except for p. aeruginosa isolated from various clinical specimens over the past 5 years were studied. acinetobacter anitratum was the most commonly encountered strain and p. maltophilia and p. putida were also frequently encountered during 1974 through 1977. in 1978, p. cepacia became a significantly predominant strain, which was 44.6% of all nonfermentative gram-negative bacilli excluding p. aeruginosa iso ... | 1979 | 259663 |
| the induction of mutants of acinetobacter calcoaceticus ncib8250 and their selection by vancomycin. | the mutagenic and lethal effects of n-methyl-n-nitro-n-nitrosoguanidine (ntg), ethyl methanesulphonate (ems), ultraviolet light iffadiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium acinetobacter calcoaceticus ncib8250 were examined. the production of auxotrophic mutants was used as a measure of mutagenic efficiency. under appropriate conditions all four agents were mutagenic. ems and ntg although more effective than irradiation, did not cause such a high fre ... | 1975 | 173792 |
| hydrolysis of phosphatidylethanolamine and phosphatidylglycerol by outer membrane phospholipase a from acinetobacter sp. ho1-n. | an outer membrane phospholipase a active against phosphatidylglycerol and phosphatidylethanolamine was characterized from acinetobacter sp. ho1-n. | 1978 | 30758 |
| prevalence and survival of microbial contaminants in heated nebulizers. | over a 2-year period, 600 cultures of fluid in heated nebulizers in use by patients in the peter bent brigham hospital were performed. the most commonly isolated microorganisms were pseudomonas and alcaligenes species. pseudomonas aeruginosa was never isolated. three types of heated nebulizers were in use, and their contamination rate was significantly different (45 percent, 21 percent, and 8 percent, respectively; p less than 0.001). in the course of the study, the overall contamination rate de ... | 1978 | 273384 |
| [kinetics of setting up of a human fecal flora in germ free mice and trial of decontamination of antibiotherapy (author's transl)]. | we have tried in this study to reproduce in germfree mice a bacterial equilibrium observed previously in the gastrointestinal tract of child "l.n.", reared in a plastic isolator. eight bacterial strains were studied. seven had been fortuitously introduced: acinetobacter lwoffi ln1, staphylococcus epidermidis ln2, clostridium perfringens ln3, plectridium anatum ln4, streptococcus bovis ln5, enterobacter cloacae ln6, corynebacterium sp ln7. the eighth strain was purposely administered to the child ... | 1975 | 174472 |
| in vitro and in vivo activities of minocycline and other antibiotics against acinetobacter (herellea-mima). | minocycline was the most active of six antibiotics tested against 65 clinical isolates of acinetobacter calcoaceticus (syn.: herellea, mima) received from six medical centers. in the bauer-kirby disk susceptibility test, all isolates were rated susceptible to minocycline, gentamicin, and polymyxin; 25% were resistant to tetracycline. in agar dilution tests, minocycline was two to four times more potent than gentamicin or polymyxin and eight times more potent than tetracycline. ampicillin and cep ... | 1976 | 176936 |
| aerobic and anaerobic flora in bladder urine of patients with indwelling urethral catheters. | twenty-five bladder urine specimens from 13 patients with long-standing indwelling catheters were obtained by percutaneous suprapubic needle aspiration to avoid contamination by extraneous flora and cultured aerobically and anaerobically. twenty different species of organism were isolated from twenty-four positive specimens. the isolates were predominantly gram-negative aerobic or facultatively anaerobic bacilli, with occasional gram-positive aerobic cocci and yeasts. one patient had clostridium ... | 1976 | 178084 |
| inhibition of the malic enzyme from acinetobacter calcoaceticus by nadph and nadh. | the malic enzyme enriched from acinetobacter calcoaceticus is inhibited by nadph and nadh. the inhibition afforded by the reduced coenzymes is not affected by nad+, amp and 3'.5'-amp. against l-malate, nadph inhibits the enzyme in a noncompetitive linear fashion (ki = 1.5 x 10(-4) m), against nadp+, competitively linearly (ki = 5.0 x 10(-5) m). while nadph acted as a product inhibitor, nadh seems to be an allosteric effector of the malic enzyme, because with l-malate as the variable substrate in ... | 1975 | 9762 |
| characterization of intracytoplasmic hydrocarbon inclusions from the hydrocarbon-oxidizing acinetobacter species ho1-n. | the ultrastructure of acinetobacter sp. strain ho1-n grown on hydrocarbon and nonhydrocarbon substrates was compared using thin sections and freeze-etching. hydrocarbon-grown cells were characterized by the presence of intracytoplasmic membrane-bound hexadecane inclusions. this membrane did not exhibit a typical unit membrane structure but appeared as a monolayer. the freeze-etch technique revealed the internal structure of the hexadecane inclusions and provided evidence for the presence of a sm ... | 1976 | 179978 |
| a comparative analysis of the ultrastructure of hydrocarbon-oxidizing micro-organisms. | the ultrastructure of a variety of micro-organisms was compared after growth on hydrocarbon and non-hydrocarbon substrates. hydrocarbon-grown organisms were characterized by the presence of intracellular electron-transparent inclusions which in many cases appeared membrane-bound. with one exception, non-hydrocarbon-grown organisms did not contain electron-transparent inclusions. insignificant amounts of poly-beta-hydroxybutyric acid were produced by the hydrocarbon-grown micro-organisms. after g ... | 1976 | 181527 |
| susceptibility of "enterobacteria" to aminoglycoside antibiotics: comparisons with tetracyclines, polymyxins, chloramphenicol, and spectinomycin. | strains of enterobacteria, including common, aerobic, pathogenic gram-negative bacilli, and enterococci were tested for susceptibility to 11 aminoglycoside antibiotics by a twofold agar-dilution method with an inocula replicator. for comparison, similar tests were done with seven tetracycline analogues, two polymyxins, chloramphenicol, and spectinomycin. tobramycin compared favorably with the more active aminoglycosides, with some exceptions related to individual strains of species. the tetracyc ... | 1976 | 184213 |
| nucleotide specificity of succinate thiokinases from bacteria. | | 1977 | 189692 |
| evidence for allosteric nadh regulation of acinetobacter alpha-oxoglutarate dehydrogenase from multiple-inhibition studies. | | 1977 | 191030 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| differentiation of nonfermentative gram-negative bacilli in the clinical laboratory. | a rapid and simplified system for the differentiation of nonfermentative gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. nonfermentative bacteria can be grouped initially by the motility, oxidase and of glucose reactions. this grouping simplifies the choice of additional tests for further identification. the additional tests included gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase act ... | 1977 | 329428 |
| microbial aggregate contamination of water lines in dental equipment and its control. | water from some dental clinics has been examined and found to be discoloured, badly tasting and with a foul odour. moreover, brown or black flakes were often present in tap water, as well as in the water lines of dental equipment. examination by phase-contrast and electron microscope showed the flakes to consist of aggregated fungi and bacteria, and similar structures were found in a layer on the inner surfaces of the clinics water tubes and pipes. the ultrastructure of some aggregating microorg ... | 1977 | 329637 |
| regulation of synthesis of benzyl alcohol dehydrogenase in acinetobacter calcoaceticus ncib8250. | specific activity of benzyl alcohol dehydrogenase in carbon-limited continuous cultures was at a maximum at a specific growth rate of 0.2 h-1, but fell off at lower and higher growth rates. the specific activity in nitrogen-limited cultures was always lower and was inversely proportional to growth rate. there was severe repression of benzyl alcohol dehydrogenase during metabolism of l(+)-mandelate or phenylglyoxylate in batch cultures. synthesis of benzyl alcohol dehydrogenase was followed in ex ... | 1977 | 201725 |
| oil tankers and pollution: a microbiological approach. | | 1977 | 334042 |
| gram-negative bacillary septic arthritis: clinical, radiographic, therapeutic, and prognostic features. | | 1977 | 337494 |
| endotoxin-induced platelet aggregation and secretion. i. morphological changes and pharmacological effects. | endotoxin lipopolysaccharide (lps) from acinetobacter 199a induced aggregation of blood platelets from immune adherence-positive species (rat, rabbit) but not from immune adherence-negative species such as pig and man. aggregation occurred in 2 phases: the first was not accompanied by secretion of platelet constituents, was apparently a consequence of c3 activation, and was selectively inhibited by egta. the second phase of aggregation was associated with secretion of platelet granule contents, ... | 1977 | 202608 |
| [kinetic and regulatory properties of malate dehydrogenase from acinetobacter calcoaceticus]. | | 1978 | 216197 |
| nadh and nadph-viologen reductases from acinetobacter calcoaceticus. | three pyridine nucleotide-dependent diaphorases have been isolated from acinetobacter calcoaceticus cells and partially characterized. two of them, with molecular weights of 165,000 and 57,000, utilize nadph as electron donor whereas the third one (mw = 57,000) is specific for nadh. oxidized viologen dyes, flavin nucleotides, dichlorophenol indophenol and ferricyanide can act with efficiency as acceptors in the reaction mediated by these diaphorases. the diaphorase activities have been character ... | 1979 | 223203 |
| differences between cephalothin and newer parenterally absorbed cephalosporins in vitro: a justification for separate disks. | the activities of cefamandole and cefoxitin in vitro were compared with that of cephalothin against staphylococci and gram-negative bacilli. cephalothin was the most active agent against staphylococci. cefamandole exhibited the greatest activity against the enterobacteriaceae, with the exceptions of serratia marcescens and indole-positive proteeae, against which cefoxitin was the most active antibiotic. the activity of newer cephalosporins that are resistant to the beta-lactamases of gram-negati ... | 1978 | 349096 |
| a comparative study of the activity of cefamandole and other cephalosporins and analysis of the beta-lactamase stability and synergy of cefamandole with aminoglycosides. | the antibacterial activity of cefamandole against 445 clinical isolates was investigated and compared with the activity of other known cephalosporins (cephalothin, cephaloridine, cephalexin, and cefazolin) and of two penicillins (ampicillin and carbenicillin). cefamandole was the most active antibiotic against isolates of citrobacter, enterobacter, and shigella, and its activity against staphylococcus aureus, bacteroides, and some members of the enterobacteriaceae was comparable to that of the o ... | 1978 | 349097 |
| [induction and repression of the collagenase synthesis in acinetobacter sp]. | the synthesis of collagenase in acinetobacter sp. was found to be inducible by denatured collagen and by its high molecular weight fragments. the presence in the inducer of part of the tertiary structure appear to be indispensable. on the other hand, an addition of casamino acids, meat protein hydrolysate, or a mixture of amino acids with a similar composition to gelatin does not stimulate collagenase synthesis. enzyme production was severely repressed in the early phase of growth by glucose, ar ... | 1979 | 224995 |
| in vitro combination of mecillinam with cephradine or amoxycillin for organisms resistant to single agents. | of 36 multi-resistant gram-negative bacilli isolated from different cases of urinary tract infection, 18 strains of escherichia coli, klebsiella, enterobacter, proteus, providencia and serratia with the exception of acinetobacter species showed synergy when mecillinam was combined with cephradine or amoxycillin in the ratio of 1:1. combination with cephradine was synergistic on sixteen occasions and amoxycillin on six. synergy, which might have clinical relevance, was demonstrated only when the ... | 1979 | 227829 |
| [attachment of a collagenolytic strain to its substrate (author's transl)]. | the bacterial collagenolytic strain acinetobacter sp. crzv2 adheres to insoluble collagen fibers when this substrate is introduced into the growth medium. this attachment occurs during the exponential growth. proteolytic enzymes such as pronase and trypsine activate the adherence of bacterial cells to collagen fibers. | 1979 | 228578 |
| inhibition of nadp-linked malic enzyme by atp in acinetobacter. | | 1975 | 235454 |
| long-term therapy of chronic bacterial prostatitis with trimethoprim-sulfamethoxazole. | trimethoprim (tmp) meets all of the theoretical requirements of diffusion into, and actual concentration in, human prostatic fluid. when tmp is combined with the sulfonamide sulfamethoxazole (smx), potentiation of antibacterial activity is achieved and the development of resistant bacterial strains is less likely to occur. in our initial use of tmp-smx in the treatment of 13 men with chronic bacterial prostatitis due to gram-negative organisms, patients were given two tablets of tmp-smx twice da ... | 1975 | 236820 |
| genetics and physiology of acinetobacter. | | 1978 | 360969 |
| nosocomial respiratory tract infection and colonization with acinetobacter calcoaceticus. epidemiologic characteristics. | nosocomial respiratory tract infection with acinetobacter calcoaceticus occurs frequently in many hospitals. an outbreak of respiratory tract infections in an intensive care unit provided an opportunity to study clinical and epidemiologic characteristics of such infections. retrospective studies demonstrated that a. calcoaceticus in sputum was significantly associated with endotracheal intubation (p = 0.03) and continuous positive pressure ventilation (p less than 0.02). after control measures h ... | 1978 | 362918 |
| outbreak of a multiply resistant acinetobacter in a surgical intensive care unit: epidemiology and control. | during january, 1976 seven patients in an sicu became colonized or infected with acinetobacter calcoaceticus (variation, anitratum) at an attack rate of 12.5 per cent. the organism showed a marked reduction in antimicrobial sensitivity from previous experience. comparison with 34 uninfected control patients indicated that intubation and continuous ventilatory assistance were significantly associated with acquiring the organism (p = 0.0154). acinetobacter was cultured from nurses' hands, ambu ada ... | 1978 | 248372 |
| [investigation in vitro synergism on ampicillin with cloxacillin and dibekacin against glucose non-fermenting gram-negative bacilli and serratia (author's transl)]. | | 1978 | 366194 |
| isolation of acinetobacter species in lusaka. | | 1979 | 263365 |
| ecological effects of a deodorant and a plain soap upon human skin bacteria. | the effects of a commercial trichlorocarbanilide-containing deodorant soap and a commercial plain soap upon the cutaneous flora of individuals were compared. using a cross-over design, 21 volunteers (10 women and 11 men) washed their forearms at least once a day with one soap for 3 weeks and then switched soaps for another 4 weeks use. by analysis of variance no significant difference in total colony counts was noted among individuals in their use of the two soaps. with the exception of individu ... | 1977 | 264493 |
| origins of metabolic diversity: evolutionary divergence by sequence repetition. | recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec 5.3.3.4] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec 3.1.1.24], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ... | 1979 | 291059 |
| [nonfermenting gram-negative bacteria. iii. acinetobacter]. | | 1979 | 377870 |
| bronchopneumonia and endocarditis: caused by acinetobacter anitratum (herellea vaginicola). | | 1979 | 291811 |
| effect of succinylated acinetobacter glutaminase-asparaginase treatment on an acute myeloid leukemia in the rat (bnml). | | 1979 | 292598 |
| gentamicin resistance in christchurch hospitals. | prior to june 1976, the isolation of gentamicin resistant organisms was an infrequent occurrence in north canterbury hospital board institutions. during july 1976, 20 different gentamicin resistant organisms were isolated from patients in christchurch hospital. gentamicin resistant organisms hav e been continually isolated from an increasingly wide area since then. the organisms involved are: providence species; pseudomonas aeruginosa; klebsiella species; e coli; staphyloccus aureus; proteus mir ... | 1979 | 381994 |
| flocculation and adsorption of enzymes during growth of a moderate halophile, micrococcus varians var. halophilus. | flocculation of a moderate halophile, micrococcus varians atcc 2197, occurred during growth in complex medium containing 3 m nacl and a concentration of mgso4 and kh2po4 greater than 40 and 14 mm, respectively. extracellular nuclease activity was absent in the flocculated cultures. repeated washing of flocs by mg2+-free tris buffer containing 3 m nacl, lowering of ph value of floc suspension below 6.3, or addition of ethylenediaminetetraacetic acid resulted in complete dissociation of the flocs ... | 1978 | 307427 |
| combined action of sulphamethoxazole and trimethoprim against clinically-isolated sulphonamide-resistant bacteria. | 42 strains of gram-negative bacteria, isolated from clinical material, resistant to trimethoprim (tm), sulphamethoxazole (smz) or both, have been tested for synergistic sensitivity to tm + smz. the combination showed no synergy against strains 'highly resistant' to smz (mic greater than 1 mg/ml). indifference was invariably observed. however, synergy was seen with strains 'moderately resistant' to smz (mic 100--1,000 microgram/ml). our results indicate that present techniques for the testing of ... | 1979 | 383418 |
| fosfomycin and plasmidic resistance. | 60 fosfomycin-resistant strains of gram-negative bacilli are submitted to conjugation experiments using as recipient cell e. coli k12 which is nalidixic acid resistant. after mating, the number of fosfomycin-resistant e. coli k2 colonies growing on selection plates containing nalidixic acid and fosfomycin never surpassed the normal rate of mutation for fosfomycin-resistance of the recipient strain. in 65% of the experiments, plasmidic resistance to other antimicrobials was transferred to e. coli ... | 1977 | 318973 |
| prosthetic valve endocarditis by opportunistic pathogens. | the incidence of endocarditis produced by the so-called "opportunists" as a complication of prosthetic valve surgery is progressively increasing in frequency and gradually transforming the clinical picture habitually associated with this disease. we report six cases of endocarditis produced by opportunistic microorganisms (two cases by candida, and the remaining by serratia, actinobacillus, acinetobacter calcoaceticus, and bacteroides fragilis, and by corynebacterium diphtheriae) in four male an ... | 1977 | 319774 |