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fine structure of the gram-negative bacterium acetobacter suboxydans.the morphological features of the cell wall, plasma membrane, protoplasmic constituents, and flagella of acetobacter suboxydans (atcc 621) were studied by thin sectioning and negative staining. thin sections of the cell wall demonstrate an outer membrane and an inner, more homogeneous layer. these observations are consistent with those of isolated, gram-negative cell-wall ghosts and the chemical analyses of gram-negative cell walls. certain functional attributes of the cell-wall inner layer and ...196414126870
on the unity of bacterial ribosomes. 196414135529
[the influence of temperature on the activity of acetobacter aceti in submerged cultures]. 196314137784
metabolism of dicarboxylic acids in acetobacter xylinum.benziman, moshe (the hebrew university of jerusalem, jerusalem, israel), and a. abeliovitz. metabolism of dicarboxylic acids in acetobacter xylinum. j. bacteriol. 87:270-277. 1964.-during the oxidation of fumarate or l-malate by whole cells or extracts of acetobacter xylinum grown on succinate, a keto acid accumulated in the medium in considerable amounts. this acid was identified as oxaloacetic acid (oaa). no accumulation of oaa was observed when succinate served as substrate. these phenomena c ...196414151044
antitumor activity of certain substances of natural origin. 196414151913
the mechanism of cacao curing. 196314153268
the differentiation of serotype a and b dextrans by means of partial acetolysis. 196414163897
oxidation of aliphatic glycols by acetic acid bacteria. 196414172022
enzymes of the tricarboxylic acid cycle in acetic acid bacteria. 196414179672
lethal action by an acetobacter on yeasts. 196414190067
mutant frequency in acetobacter. 196414191596
valine-isoleucine metabolism in acetobacter suboxydans and the inhibition of growth by valine.kerwar, suresh s. (oregon state university, corvallis), vernon, h. cheldelin, and l. w. parks. valine-isoleucine metabolism in acetobacter suboxydans and the inhibition of growth by valine. j. bacteriol. 88:179-186. 1964.-extracts of acetobacter suboxydans can synthesize valine and isoleucine via acetolactate and acetohydroxybutyrate, respectively. the amounts of these amino acids synthesized from different intermediates were determined. the pathways appear to be identical to those described for ...196414197885
enzymatic activities in acetobacter suboxydans. ii. production of 5-ketogluconate. 196414202651
[use of acetic acid by bacteria of the acetobacter genus]. 196414206070
utilization of l and d isomers of pantothenate and pantoate for microbial growth. 196414206904
conditions of formation of idonate-gluconate. 196414209871
effect of mutation on dna-composition of some bacteria. 196414218440
flavine adenine dinucleotide-linked malic dehydrogenase from acetobacter xylinum.benziman, moshe (the hebrew university of jerusalem, jerusalem, israel), and y. galanter. flavine adenine dinucleotide-linked malic dehydrogenase from acetobacter xylinum. j. bacteriol. 88:1010-1018. 1964.-the properties of the pyridine nucleotide-nonlinked malic dehydrogenase of acetobacter xylinum were investigated in the supernatant fluid obtained by high-speed centrifugation of sonic extracts. ferricyanide, phenazine methosulfate, and to a lesser extent dichlorophenolindophenol were active a ...196414219012
deoxyribonucleic acid hybrids of acetic acid bacteria.de ley, j. (state university, ghent, belgium), and s. friedman. deoxyribonucleic acid hybrids of acetic acid bacteria. j. bacteriol. 88:937-945. 1964.-deuterated n(15)-labeled deoxyribonucleic acid (dna) from acetobacter aceti (mesoxydans 4) forms hybrids with ordinary dna from other species of this genus (a. xylinum, a. pasteurianus, a. estunensis, and possibly a. xylinoides) when the guanine plus cytosine base composition does not vary by more than 1 to 2%. beyond this limit (a. aceti ch31 and ...196414219057
the shape of the tips of growing bacterial cellulose microfibrils and its relation to the mechanism of cellulose biosynthesis. 196414222658
[changes in the resistance of chick embryos to various infections under the influence of polysaccharides from nonpathogenic microorganisms]. 196414225833
pigment formation by acetobacter acetigenum in lactate-buffered glycerol medium. 196414228895
polyhydric alcohols in human urine. 196414229543
[continuous submerged culture of acetobacter aceti in a synthetic medium]. 196414238149
[comparisons and evaluation of some microbiological methods for the determination of p-aminobenzoic acid]. 196414240456
oxaloacetate decarboxylation and oxaloacetate-carbon dioxide exchange in acetobacter xylinum.benziman, moshe (the hebrew university of jerusalem, jerusalem, israel), and n. heller. oxaloacetate decarboxylation and oxaloacetate-carbon dioxide exchange in acetobacter xylinum. j. bacteriol. 88:1678-1687. 1964.-extracts of acetobacter xylinum, prepared by sonic treatment, were shown to catalyze the decarboxylation of oxaloacetate (oaa) to pyruvate and co(2), and the exchange of c(14)-carbon dioxide into the beta-carboxyl of oaa. fractionation of the extracts with ammonium sulfate resulted i ...196414240957
[adaptive property of the pentose cycle in acetobacter xylinum]. 196514278851
polyol dehydrogenases of gluconobacter oxydans. 196514284764
[some problems in the morphology of acetobacter aceti. ii. on the flagella of acetobacter and the importance of this feature for systematology]. 196414296564
[some problems in the morphology of acetobacter aceti. i. on the involution forms of acetobacter aceti]. 196414298262
[carbohydrate requirement of acetic acid bacteria]. 196514299878
5-keto-d-fructose. i. chemical characterization and analytical determination of the dicarbonylhexose produced by gluconobacter cerinus. 196514304828
5-keto-d-fructose. ii. patterns of formation and of associated dehydrogenase activities in gluconobacter cerinus. 196514304829
beer-spoilage bacteria and their control with a phosphoric acid--ammonium persulfate wash. 196514332530
[influence of the nature of the carbon source utilized during growth on biosynthesis of different enzymes in "acetobacter xylinum"]. 196514337121
localisation and distribution of alcohol-cytochrome 553 reductase in acetic acid bacteria. 196514340586
identification of altro-heptulose and l-threitol as products of meso-erythritol oxidation by acetobacter suboxydans. 196514342732
acetolactate metabolism and the presence of a dihydroxy acid dehydratase in micro-organisms.1. the growth characteristics of nine micro-organisms on complex broth and defined media, usually with a single nitrogen source (other than vitamins), were examined as a necessary step before growth of cells for enzyme assays. six of these bacteria gave a positive colour test with a creatine-potassium hydroxide reagent, indicating the presence of acetoin, which other investigators have shown is formed via the intermediate, alpha-acetolactate. 2. cell-free extracts of exponential-phase cells of b ...196514348203
alternate conversions of glycerol to dihydroxyacetone in acetobacter sub-oxydans. 195514367358
oxidation of dihydroxyacetone via the pentose cycle in acetobacter sub-oxydans. 195514367359
[effect of penicillin and aureomycin on saccharomyces cerevisiae, thermobacterium cereale and acetobacter rancens]. 195514387590
formation of arabinose, ribulose and tartronic acid from 2-keto-d-gluconic acid. 195514389236
[effect of aeration on multiplication and oxidation of acetobacter suboxydans]. 195514393592
transglucosidase activity in acetobacter xylinum. 196014410020
[on phylogenic connections in acetic bacteria]. 196014418011
[physiology of bacteria used in rapid production of vinegar and their position in the taxonomy of the family acetobacter]. 195914418012
[presence of 2 systems for the oxidation of acetaldehyde into acetic acid in acetobacter xylinum]. 196014434886
[on the significance of the concentration of substances in contrast media in the oxidation of sorbitol by acetic bacteria]. 195914436690
derivation of non-acetifying "quasiacetobacters" from a true acetobacter strain, and vice versa. 196014446019
[a method for the control of the inoculum during biological oxidation of sorbitol into sorbose]. 196014446257
[new data on the metabolism, systematology and evolution of acetic acid bacteria]. 196214451131
ribose and nucleic-acid bases in glucose-metabolizing, washed-cell suspensions of acetobacter xylinum and their relation to cellulos synthesis. 196214455535
a new variety of acetobacter rancens occured as contamination in alcoholic fermentation. 196114457616
effect of pantothenic acid on acetobacter xylinum. 196114460853
[electron microscopic observations on the flagellae of acetic acid bacteria]. 196214466363
the biochemical preparation of d-xylulose and l-ribulose. details of the action of acetobacter suboxydans on d-arabitol, ribitol and other polyhydroxy compounds. 196214476469
[effect of the bacterial polysaccharide acetoxan on transplanted tumors]. 196214478627
synthesis of cellulose by acetobacter xylinum. v. ultrastructure of polymer.appearance of cellulose microfibrils in the medium of a suspension of cells of acetobacter xylinum in buffered glucose solution was preceded by a stage during which the cellulose in the medium was amorphous within the available resolution. the size of the vertical axis of the microfibrils of the bacterial cellulose was found on the basis of measurement of shadow length to be only about 16 a. in good agreement with findings of earlier workers, the size of the lateral axis ("width") of the image o ...196214481343
formation of d-glucose, cellobiose, and other saccharides during synthesis of cellulose by acetobacter acetigenum in a lactate-buffered glycerol medium. 196214490080
characterisation of plasmids purified from acetobacter pasteurianus 2374.four cryptic plasmids pap1, pap2, pap3, and pap4 with their replication regions ap were isolated from gram-negative bacteria acetobacter pasteurianus 2374 and characterised by sequence analyses. all plasmids were carrying the kanamycin resistance gene. three of four plasmids pap2, pap3, and pap4 encode an enzyme that confers ampicillin resistance to host cells. moreover, the tetracycline resistance gene was identified only in pap2 plasmid. all plasmids are capable to coexist with each other in a ...200314511653
study of process variables in industrial acetic fermentation by a continuous pilot fermentor and response surfaces.the modeling and optimization of industrial processes requires an intensive study of the factors involved. in this work, a continuous pilot system for studying the industrial process of acetic fermentation is developed. a doehlert design is applied to the five variables involved in the pilot process. this experimental design allows reduction of the experimental burden and the maximum amount of information to be obtained, studying the factors at different levels depending on their significance. t ...200314524708
microbial cellulose as a specialty chemical.microbial polysaccharides are extensively used commercially as gelling or suspending agents, as protective colloids or as thickening agents. until recently, microbial cellulose producing systems such as acetobacter xylinum, had been used largely as model systems for the study of cellulose biosynthesis. current advances in molecular biology and biochemical engineering promise to usher microbial cellulose into the specialty chemical market. this review will highlight some of the recent progress ma ...199014546639
utilization of the buffering capacity of corn steep liquor in bacterial cellulose production by acetobacter xylinum.acetobacter xylinum bpr2001 produces water-insoluble bacterial cellulose (bc). using a ph sensor for the accurate control of ph, which is one of the most critical factors for efficient bc production, is difficult especially in a baffled shake-flask and an airlift reactor. the buffering capacity of corn steep liquor (csl) was estimated by measuring beta (buffering capacity) values in advance and was used to maintain the ph within the optimal range during the production of bc. when csl was added t ...200414564490
an assessment of the genotoxicity and human health risk of topical use of kojic acid [5-hydroxy-2-(hydroxymethyl)-4h-pyran-4-one].kojic acid (ka), a natural substance produced by fungi or bacteria, such as aspergillus, penicillium or acetobacter spp, is contained in traditional japanese fermented foods and is used as a dermatological skin-lightening agent. high concentrations of ka (>or=1000 microg/plate) were mutagenic in s. typhimurium strains ta 98, ta 100, ta 1535, ta102 and e. coli wp2uvra, but not in ta 1537. an ames test following the "treat and plate" protocol was negative. a chromosome aberration test in v79 cells ...200414630133
acetobacter turbidans alpha-amino acid ester hydrolase: merohedral twinning in p21 obscured by pseudo-translational ncs.the structure elucidation of the alpha-amino acid ester hydrolase from acetobacter turbidans by molecular replacement is described. in the monoclinic crystal, the molecules are related by both rotational and pseudo-crystallographic translational ncs (non-crystallographic symmetry). refinement of the structure converged at unacceptably high r factors. after re-evaluation of the data, it was found that the crystal was merohedrally twinned, with a high twinning fraction. it is shown that the pseudo ...200314646082
macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy.the macromolecular structure of purified cellulose samples is studied by second-harmonic generation (shg) imaging microscopy. we show that the shg contrast in both valonia and acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. polarization analysis shows that microfibrils in each lamella are highly aligned and ordered and change directions by 90 degrees in adjacent lamellae. the angular dependence of the shg int ...200314649943
improvement of cotton fiber quality by transforming the acsa and acsb genes into gossypium hirsutum l. by means of vacuum infiltration.a novel method for the genetic transformation of cotton pollen by means of vacuum infiltration and agrobacterium-mediated transformation is reported. the acsa and acsb genes, which are involved in cellulose synthesis in acetobacter xylinum, were transferred into pollen grains of brown cotton with the aim of improving its fiber quality by incorporating useful prokaryotic features into the colored cotton plants. transformation was carried out in cotton pollen-germinating medium, and transformation ...200414740167
new cationic exchanger support for reversible immobilization of proteins.new tailor-made cationic exchange resins have been prepared by covalently binding aspartic-dextran polymers (e.g. mw 15 000-20 000) to porous supports (aminated agarose and sepabeads). more than 80% of the proteins contained in crude extracts from escherichia coli and acetobacter turbidans have been strongly adsorbed on these porous materials at ph 5. this interaction was stronger than in conventional carboxymethyl cellulose (e.g., at ph 7 and 25 degrees c, all proteins previously adsorbed at ph ...200414763854
[oxidation by acetobacter. 8. technic of bacterial oxidation in subsurface culture]. 195014777792
[oxidation by acetobacter. 9. formation of oxogluconic acid by acetobacter melanogenum]. 195014777793
[the acetobacters]. 195014785697
[oxidation by acetobacter. 10. oxidation of enantiomorphic forms of xylose and arabinose]. 195014791374
[effect of partial pressure of oxygen on the oxidation of sorbite into sorbose by bacteria acetobacter melanogenum]. 195014820957
formation of cellulose by certain species of acetobacter. 195114838912
formation of 2-keto-d-gluconic acid, 5-keto-d-gluconic acid, and tartronic acid by acetobacter species. 195114843105
[the sulfur-removing ability of some homolactic microorganisms when provided and when not provided with hematin ferments]. 195214904467
[effect of histidine on oxidation of sorbite by acetobacter melanogenum]. 195214906226
[studies on acetic fermentation. iii. effect of aeration on submerged fermentation]. 195114906335
bacterial enzyme preparations oxidizing inositol and their inhibition by colchicine. 195114910609
oxidative dissimilation of non-nitrogenous compounds in acetobacter suboxydans. 195214913149
[rate of cell multiplication in plicated form of acetobacter suboxydans]. 195214926629
on the presence of acetobacter oxydans in apple juice. 195214927887
[carbon dioxide requirement in bacteria oxidizing sorbitol into sorbose]. 195214940713
[relation between acetobacter and anguillala aceti]. 195214940724
insertion of an e. coli lacz gene in acetobacter xylinus for the production of cellulose in whey.a mini-tn10:lacz:kan was inserted into a wild-type strain of acetobacter xylinus by random transposon mutagenesis, generating a lactose-utilising and cellulose-producing mutant strain designated itz3. antibiotic selection plate assays and southern hybridisation revealed that the lacz gene was inserted once into the chromosome of strain itz3 and was stably maintained in non-selective medium after more than 60 generations. the modified strain had, on the average, a 28-fold increase in cellulose pr ...200414987772
cellulose biosynthesis: exciting times for a difficult field of study.the past few decades have witnessed exciting progress in studies on the biosynthesis of cellulose. in the bacterium acetobacter xylinum, discovery of the activator of the cellulose synthase, cyclic diguanylic acid, opened the way for obtaining high rates of in vitro synthesis of cellulose. this, in turn, led to purification of the cellulose synthase and for the cloning of genes that encode the catalytic subunit and other proteins that bind the activator and regulate its synthesis and degradation ...199915012210
application of molecular methods for the differentiation of acetic acid bacteria in a red wine fermentation.to apply rapid and reliable molecular techniques for typing acetic acid bacteria and studying their population dynamics during wine-making processes.200415012825
structural changes in the cells of some bacteria during population growth: a fourier transform infrared-attenuated total reflectance study.structural changes occurring in the cells of several bacteria during their growth curves have been investigated by fourier transform infrared (ft-ir) spectroscopy using the sampling technique of attenuated total reflectance (atr). spectra reflect all of the components of the cells, including the cell walls, cell membranes, internal structures, and the cytoplasm. the bacteria studied were bacillus stearothermophilus, halobacterium salinarum, halococcus morrhuae, and acetobacter aceti. all species ...200415035713
features of bacterial cellulose synthesis in a mutant generated by disruption of the diguanylate cyclase 1 gene of acetobacter xylinum bpr 2001.the diguanylate cyclase 1 (dgc1) (dgc1) gene in acetobacter xylinum bpr 2001--a bacterial cellulose (bc) producer--was cloned and sequenced, and a dgc1 gene-disrupted mutant, strain dd, was constructed. the production and structural characteristics of the bc formed by dd were compared with those of the parental strain bpr 2001. bc production by dd was almost the same as that by bpr 2001 in static cultivation and in shake flask cultivation. however, in a jar fermentor dd produced about 36% more b ...200415042328
mechanical effects of plant cell wall enzymes on cellulose/xyloglucan composites.xyloglucan-acting enzymes are believed to have effects on type i primary plant cell wall mechanical properties. in order to get a better understanding of these effects, a range of enzymes with different in vitro modes of action were tested against cell wall analogues (bio-composite materials based on acetobacter xylinus cellulose and xyloglucan). tomato pericarp xyloglucan endo transglycosylase (txet) and nasturtium seed xyloglucanase (nxgase) were produced heterologously in pichia pastoris. the ...200415053757
in vitro evaluation of physiological activity of vinegar produced from barley-, sweet potato-, and rice-shochu post-distillation slurry.vinegar was produced from barley-, sweet potato-, and rice-shochu post-distillation slurry using jar fermentor within 19 hrs. all the vinegars showed radical-scavenging activity, angiotensin i converting enzyme (ace) inhibition and advanced glycation endproducts (age) inhibition in vitro. the radical-scavenging activity of the vinegar produced from sweet potato-shochu post-distillation slurry was higher than that of other two kinds of vinegar on the organic matter basis. the ace inhibitory activ ...200415056886
identification of acetic acid bacteria isolated from fruits collected in thailand. 200415057711
antimicrobial and antioxidant flavonoids from the root wood of bolusanthus speciosus.three new flavonoids-5,7,4'-trihydroxy-6-[1-hydroxy-2-methylbuten-2-yl]isoflavone (isogancaonin c), 7,2'-dihydroxy-4'-methoxyisoflav-3-ene (bolusanthin iii), 6,6'-dihydroxy-4'-methoxy-2-arylbenzofuran (bolusanthin iv), in addition to eight known flavonoids; derrone, medicarpan, genistein, wighteone, lupiwighteone, gancaonin c, 7-hydroxy-4'-methoxyisoflavone and 7,3'-dihydroxy-4'-methoxyisoflavone were isolated from the root wood of bolusanthus speciosus. the compounds showed strong antimicrobial ...200415081287
significance of effector protease receptor-1 expression and its relationship with proliferation and apoptotic index in patients with primary advanced gastric adenocarcinoma.to investigate the expression of effector protease receptor-1 (epr-1), proliferative index ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of epr-1 expression and its correlationship with the proliferation and apoptosis indexes.200415112339
emendation of the genus acidomonas urakami, tamaoka, suzuki and komagata 1989.the genus acidomonas and the species acidomonas methanolica were recharacterized by using the type strain (nric 0498(t)), three reference strains and 10 methanol-utilizing bacteria that were isolated from activated sludge from three different sewage-treatment plants in tokyo. based on 16s rdna sequences, all strains formed a single cluster within the acetobacteraceae that was clearly different from the genera acetobacter, gluconobacter, gluconacetobacter, asaia and kozakia: the 14 strains were i ...200415143037
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
enhanced expression of aconitase raises acetic acid resistance in acetobacter aceti.acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. two-dimensional gel electrophoretic analysis of a soluble fraction of acetobacter aceti revealed the presence of several proteins whose production was enhanced, to various extents, in response to acetic acid in the medium. a protein with an apparent molecular mass of 100 kda was significantly enhanced in amount by acetic acid and identifi ...200415183880
bacterial cellulose as a potential scaffold for tissue engineering of cartilage.tissue constructs for cartilage with native mechanical properties have not been described to date. to address this need the bacterial cellulose (bc) secreted by gluconacetobacter xylinus (= acetobacter xylinum) was explored as a novel scaffold material due to its unusual material properties and degradability. native and chemically modified bc materials were evaluated using bovine chondrocytes. the results indicate that unmodified bc supports chondrocyte proliferation at levels of approximately 5 ...200515275816
swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (porteresia coarctata tateoka).a novel species, swaminathania salitolerans gen. nov., sp. nov., was isolated from the rhizosphere, roots and stems of salt-tolerant, mangrove-associated wild rice (porteresia coarctata tateoka) using nitrogen-free, semi-solid lgi medium at ph 5.5. strains were gram-negative, rod-shaped and motile with peritrichous flagella. the strains grew well in the presence of 0.35% acetic acid, 3% nacl and 1% kno3, and produced acid from l-arabinose, d-glucose, glycerol, ethanol, d-mannose, d-galactose and ...200415280289
yeast ecology of kombucha fermentation.kombucha is a traditional fermentation of sweetened tea, involving a symbiosis of yeast species and acetic acid bacteria. despite reports of different yeast species being associated with the fermentation, little is known of the quantitative ecology of yeasts in kombucha. using oxytetracycline-supplemented malt extract agar, yeasts were isolated from four commercially available kombucha products and identified using conventional biochemical and physiological tests. during the fermentation of each ...200415282124
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