Publications

TitleAbstractYear
Filter
PMID
Filter
a positive regulatory role for ljerf1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of lotus japonicus.we have used reverse genetics to identify genes involved in legume-rhizobium symbiosis in lotus japonicus. we obtained the sequences of 20 putative transcription factors from previously reported large-scale transcriptome data. the transcription factors were classified according to their dna binding domains and patterns of expression during the nodulation process. we identified two homologues of medicago truncatula mthap2-1, which encodes a ccaat-binding protein and has been shown to play a role ...200818567832
transformation of kalanchoe blossfeldiana with rol-genes is useful in molecular breeding towards compact growth.dwarf genotypes of the economically important flowering potted plant kalanchoe blossfeldiana were developed by molecular breeding. root inducing (ri)-lines were regenerated by applying cppu to the hairy roots, which were produced by inoculating leaf explants with a wild-type agrobacterium rhizogenes strain atcc15834. amplification by polymerase chain reaction (pcr) and southern blot analysis confirmed the presence of t-dna in the ri-lines. six ri-lines were characterised in a greenhouse trial re ...200818597094
rhizobium multihospitium sp. nov., isolated from multiple legume species native of xinjiang, china.thirty-one rhizobial strains isolated from nodules of legumes native of xinjiang, china, were characterized. these strains were classified as belonging to the genus rhizobium based on amplified 16s rdna restriction analysis (ardra). the strains were distinguished from recognized rhizobium species using analysis of 16s-23s rdna intergenic spacers (igs-rflp), sds-page analysis of whole proteins and box-pcr; the test strains always formed a distinct cluster with patterns that were quite different f ...200818599718
studies on the optimization of growth and indole alkaloid production by hairy root cultures of catharanthus roseus.catharanthus roseus hairy root cultures, genetically transformed with agrobacterium rhizogenes, produce a wide variety of indole alkaloids. the effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of c. roseus hairy root cultures were studied by using statistical experimental designs and linear regression analysis. contradictory effects of these nutrients on growth and indole alkaloid production were found. the maximal growth was obtained by ...199118600658
production of indole alkaloids by selected hairy root lines of catharanthus roseus.hairy root cultures of catharanthus roseus were established by infection of seedlings with agrobacterium rhizogenes 15834. approximately 150 transformants from four different. c. roseus cultivars were screened for desirable traits in growth and indole alkaloid production. five hairy root clones grew well in liquid culture with doubling times similar to those reported for cell suspensions. fast growing clones had similar morphologies, characterized by thin, straight, and regular branches with thi ...199318609590
[calcium-dependent mechanism of somatic embryogenesis in oncogene rolc expressing cell cultures of panax ginseng].it was shown earlier, that ginseng embryogenic cell culture 2c3 was obtained as a result of callus cells transformation with the agrobacterium rhizogenes rolc oncogene. in the present report we determine that inhibitors of ca2+-channels (lacl3, verapamil, niflumic acid) certainly lowered the quantity of somatic embryos in the 2c3 cell culture. this is the evidence of the influence of calcium-dependent signal system on plant embryogenesis. protein kinases inhibitors w7 and h7 also caused the lowe ...200818610836
the rpg gene of medicago truncatula controls rhizobium-directed polar growth during infection.rhizobia can infect roots of host legume plants and induce new organs called nodules, in which they fix atmospheric nitrogen. infection generally starts with root hair curling, then proceeds inside newly formed, intracellular tubular structures called infection threads. a successful symbiotic interaction relies on infection threads advancing rapidly at their tips by polar growth through successive cell layers of the root toward developing nodule primordia. to identify a plant component that cont ...200818621693
[induction and in vitro culture of hairy roots of solanum nigrum l. var. pauciflorum liou and its solasodine production].by using genetic transformation of agrobacterium rhizogenes and liquid culture, induction and culture conditions for solanum nigrum l. var. pauciflorum liou hairy roots and its solasodine production and consumption changes of n resource and calcium in the medium during liquid culture were investigated. the results showed that hairy roots could be initiated from the cut edges of leaf explants 5 days after inoculation with the strain of a. rhizogenes atcc15834. the percentage of rooted leaf explan ...200818630597
production of monoclonal antibodies by tobacco hairy roots.hairy roots of tobacco (nicotiana tabacum) were used to produce full-length murine lgg(1) monoclonal antibody. the presence of heavy (gamma) and light (kappa) chains and fully assembled antibody was verified by western blot analysis of root extracts. antibody levels in the biomass and medium were quantified by elisa based on detection of gamma-kappa complexes. antibody produced by hairy roots was fully functional as demonstrated in bacterial aggregation assays which confirmed bivalent antigen-bi ...199718634133
a nodule-specific plant cysteine proteinase, asnodf32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume astragalus sinicus.asnodf32, encoding a nodule-specific cysteine proteinase in astragalus sinicus, is probably involved in nodule senescence. to obtain direct evidence of its role in nodule senescence, agrobacterium rhizogenes-mediated rna interference was applied to a. sinicus hairy roots. real-time qrt-pcr was used to estimate the efficiency of suppression. the senescent phenotype of transgenic nodules was examined with paraffin-embedded slides, tunel (tdt-mediated dutp nick-end labeling) assay, and transmission ...200818643938
transgenic mimicry of pathogen attack stimulates growth and secondary metabolite accumulation.plant secondary metabolites, including pharmaceuticals, flavorings and aromas, are often produced in response to stress. we used chemical inducers of the pathogen defense response (jasmonic acid, salicylate, killed fungi, oligosaccharides and the fungal elicitor protein, cryptogein) to increase metabolite and biomass production in transformed root cultures of the medicinal plant, withania somnifera, and the weed, convolvulus sepium. in an effort to genetically mimic the observed effects of crypt ...200918668338
establishment of withania somnifera hairy root cultures for the production of withanolide a.withania sominifera (indian ginseng) was transformed by agrobacterium rhizogenes. explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with a. rhizogenes strain r1601. hairy (transformed) roots were induced from cotyledons and leaf explants. the transgenic status of hairy roots was confirmed by polymerase chain reaction using nptii and rolb specific primers and, subsequently, by southern analysis for the presence of nptii and r ...200818713347
regeneration of foreign genes co-transformed plants of medicago sativa l by agrobacterium rhizogenes.gene encoding sulphur amino acid-rich protein (hnp) and rol genes were transferred into medicago sativa l (alfalfa) mediated by agrobacterium tumafeciens. regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. cotyledon tissues were an ideally transformed recipient. there was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. production of co-transformed plants with greater yield and super quality was impo ...200018726342
arabidopsis tonneau1 proteins are essential for preprophase band formation and interact with centrin.plant cells have specific microtubule structures involved in cell division and elongation. the tonneau1 (ton1) mutant of arabidopsis thaliana displays drastic defects in morphogenesis, positioning of division planes, and cellular organization. these are primarily caused by dysfunction of the cortical cytoskeleton and absence of the preprophase band of microtubules. characterization of the ton1 insertional mutant reveals complex chromosomal rearrangements leading to simultaneous disruption of two ...200818757558
[construction of a novel vector harboring green fluorescence protein gene (gfp) and high expression of gfp in transformed roots of petunia hybrida].a novel vector pbin-35s-gfp was constructed from the plasmids of pbin19, pgfp, and pchs, which included gfp gene driven by the camv 35s promoter. the hairy roots of petunia hybrida were induced by wild-type agrobacterium rhizogenes k599 harboring pbin-35s-gfp with the frequency of 45%. the pcr results showed that rolb from k599 ri plasmid and gfp from pbin-35s-gfp were co-transformed into the genome of p. hybrida. the high activity of green fluo-rescence protein was detected by fluorescence micr ...200818779160
assessment of the nutritional values of genetically modified wheat, corn, and tomato crops.the genetic modification in fruit and vegetables could lead to changes in metabolic pathways and, therefore, to the variation of the molecular pattern, with particular attention to antioxidant compounds not well-described in the literature. the aim of the present study was to compare the quality composition of transgenic wheat ( triticum durum l.), corn ( zea mays l.), and tomato ( lycopersicum esculentum mill.) to the nontransgenic control with a similar genetic background. in the first experim ...200818781763
stress tolerance, genetic analysis and symbiotic properties of root-nodulating bacteria isolated from mediterranean leguminous shrubs in central spain.nine root-nodulating bacterial isolates were obtained from the leguminous shrubs spartium junceum, adenocarpus hispanicus, cytisus purgans, cytisus laburnuum, retama sphaerocarpa and colutea arborescens in areas of central spain. a poliphasic approach analyzing phenotypic, symbiotic and genetic properties was used to study their diversity and characterize them in relation to mediterranean conditions. stress tolerance assays revealed marked variations in salinity, extreme ph and cadmium tolerance ...200918784916
diterpenoid production in hairy root culture of salvia sclarea l.growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a salvia sclarea hairy root culture are described. the roots transformed by agrobacterium rhizogenes (lba 9402) were cultured in half-strength b5 liquid medium supplemented with 30 g l(-1) sucrose under light (16 h/8 h light/dark). a culture period of 30 days was optimal for both biomass and diterpenoid production. the total content of four diterpenoids in the hairy roots ...200818811013
root-secreted malic acid recruits beneficial soil bacteria.beneficial soil bacteria confer immunity against a wide range of foliar diseases by activating plant defenses, thereby reducing a plant's susceptibility to pathogen attack. although bacterial signals have been identified that activate these plant defenses, plant metabolites that elicit rhizobacterial responses have not been demonstrated. here, we provide biochemical evidence that the tricarboxylic acid cycle intermediate l-malic acid (ma) secreted from roots of arabidopsis (arabidopsis thaliana) ...200818820082
abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in medicago truncatula.nodulation is tightly regulated in legumes to ensure appropriate levels of nitrogen fixation without excessive depletion of carbon reserves. this balance is maintained by intimately linking nodulation and its regulation with plant hormones. it has previously been shown that ethylene and jasmonic acid (ja) are able to regulate nodulation and nod factor signal transduction. here, we characterize the nature of abscisic acid (aba) regulation of nodulation. we show that application of aba inhibits no ...200818931020
mechanism of infection thread elongation in root hairs of medicago truncatula and dynamic interplay with associated rhizobial colonization.in temperate legumes, endosymbiotic nitrogen-fixing rhizobia gain access to inner root tissues via a specialized transcellular apoplastic compartment known as the infection thread (it). to study it development in living root hairs, a protocol has been established for medicago truncatula that allows confocal microscopic observations of the intracellular dynamics associated with it growth. fluorescent labeling of both the it envelope (atpip2;1-green fluorescent protein) and the host endoplasmic re ...200818931145
improved podophyllotoxin production by transformed cultures of linum album.various cell and hairy root cultures of l. album were developed and analyzed for podophyllotoxin content. transformed callus and hairy root cultures developed from infection of stem portions of in vitro-germinated l. album plant with agrobacterium rhizogenes ncim 5140 strain were selected on the basis of high podophyllotoxin content and growth. based on the integration of ri t(l)-dna and t(r)-dna, integration of only the ags and not the rol gene in transformed cell culture indicated fragmented i ...200818932162
the agrobacterium rhizogenes galls gene encodes two secreted proteins required for genetic transformation of plants.agrobacterium tumefaciens and agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. single-stranded dna (t strands) and virulence proteins are translocated into plant cells by a type iv secretion system. vird2 nicks a specific dna sequence, attaches to the 5' end, and pilots the dna into plant cells. a. tumefaciens translocates single-stranded dna-binding protein vire2 int ...200918952790
the agrobacterium rhizogenes galls gene encodes two secreted proteins required for genetic transformation of plants.agrobacterium tumefaciens and agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. single-stranded dna (t strands) and virulence proteins are translocated into plant cells by a type iv secretion system. vird2 nicks a specific dna sequence, attaches to the 5' end, and pilots the dna into plant cells. a. tumefaciens translocates single-stranded dna-binding protein vire2 int ...200918952790
efd is an erf transcription factor involved in the control of nodule number and differentiation in medicago truncatula.mechanisms regulating legume root nodule development are still poorly understood, and very few regulatory genes have been cloned and characterized. here, we describe efd (for ethylene response factor required for nodule differentiation), a gene that is upregulated during nodulation in medicago truncatula. the efd transcription factor belongs to the ethylene response factor (erf) group v, which contains ern1, 2, and 3, three erfs involved in nod factor signaling. the role of efd in the regulation ...200818978033
suppression of reactive oxygen species and enhanced stress tolerance in rubia cordifolia cells expressing the rolc oncogene.it is known that expression of the agrobacterium rhizogenes rolc gene in transformed plant cells causes defense-like reactions, such as increased phytoalexin production and expression of pathogenesis-related proteins. in the present study, we examined whether this phenomenon is associated with increased production of reactive oxygen species (ros). single-cell assays based on confocal microscopy and fluorogenic dyes (2,7-dichlorofluorescein diacetate and dihydrorhodamine 123) showed reduced stead ...200818986252
the aux1 gene of the ri plasmid is sufficient to confer auxin autotrophy in tobacco by-2 cells.tobacco (nicotiana tabacum) bright yellow-2 (by-2) cells are rapidly proliferating meristematic cells that require auxin for culture in vitro. we have established several transgenic by-2 cell lines that carry the t-dna of agrobacterium rhizogenes 15834, which harbors an agropine-type root-inducing (ri) plasmid. two of these lines, byhr-3 and byhr-7, were used to test the role of auxin in the proliferation of plant cells. the lines grew rapidly in linsmaier-skoog (ls) medium lacking auxin and oth ...200918986729
gs52 ecto-apyrase plays a critical role during soybean nodulation.apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphates and nucleoside diphosphates to nucleoside monophosphates and orthophosphates. gs52, a soybean (glycine soja) ecto-apyrase, was previously shown to be induced very early in response to inoculation with the symbiotic bacterium bradyrhizobium japonicum. overexpression of the gs52 ecto-apyrase in lotus japonicus increased the level of rhizobial infection and enhanced nodulation. these data suggest a ...200919036836
mitragyna speciosa: hairy root culture for triterpenoid production and high yield of mitragynine by regenerated plants.hairy root cultures of mitragyna speciosa were established by infection of agrobacterium rhizogenes atcc 15834 and maintained in mccown woody plant medium (wpm) supplemented with 0.5 mg/1 naphthaleneacetic acid. the hairy roots were identified for the rooting genes loci of rola and rolb by polymerase chain reaction. for studying the secondary metabolite production, the n-hexane extract of the hairy roots was prepared and the compounds were isolated by silica gel column chromatography, affording ...200819040109
phenylalanine ammonia-lyase and stilbene synthase gene expression in rolb transgenic cell cultures of vitis amurensis.transformation of vitis amurensis callus culture by the plant oncogene rolb of agrobacterium rhizogenes results in high (up to 3.15% dry wt.) levels of resveratrol in the transformed culture. the present study deals with the effect of rolb on phenylalanine ammonia-lyase (pal) and stilbene synthase (sts) gene expression in two rolb transgenic v. amurensis callus cultures with different levels of rolb expression and resveratrol production. the total expression of pals and stss in rolb transgenic c ...200919043702
agrobacterium rhizogenes mediated-transformation of asimina triloba l. cuttings.the current research compared rooting of pawpaw, with softwood cuttings from mature stands, cuttings from the terminal 3-4 inches of seedlings and cuttings taken from shoots of rooted cuttings from seedlings. four treatments were used: agrobacterium rhizogenes strain msu-1 (a4 wild type), a. rhizogenes strain mt232 (tr105 mutant), indole-3-butyric acid at 20,000 mg l(-1) and control. only the a. rhizogenes treatments induced rooting and only of the seedling cuttings. no statistical differences i ...200719069999
genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina).a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ...200919074602
genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina).a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ...200919074602
acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications.acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for the industrial recovery of copper (bioleaching or biomining). it is a chemolithoautrophic, gamma-proteobacterium using energy from the oxidation of iron- and sulfur-containing minerals for growth. it thrives at extremely low ph (ph 1-2) and fixes both carbon and nitrogen from the atmosphere. it solubilizes copper and other metals from rocks and plays an important role in nutrient and metal biogeochemica ...200819077236
hairy-root organ cultures for the production of human acetylcholinesterase.human cholinesterases can be used as a bioscavenger of organophosphate toxins used as pesticides and chemical warfare nerve agents. the practicality of this approach depends on the availability of the human enzymes, but because of inherent supply and regulatory constraints, a suitable production system is yet to be identified.200819105816
lotus japonicus castor and pollux are ion channels essential for perinuclear calcium spiking in legume root endosymbiosis.the mechanism underlying perinuclear calcium spiking induced during legume root endosymbioses is largely unknown. lotus japonicus symbiosis-defective castor and pollux mutants are impaired in perinuclear calcium spiking. homology modeling suggested that the related proteins castor and pollux might be ion channels. here, we show that castor and pollux form two independent homocomplexes in planta. castor reconstituted in planar lipid bilayers exhibited ion channel activity, and the channel charact ...200819106374
the ntami1 gene functions in cell division of tobacco by-2 cells in the presence of indole-3-acetamide.tobacco (nicotiana tabacum) bright yellow-2 (by-2) cells can be grown in medium containing indole-3-acetamide (iam). based on this finding, the ntami1 gene, whose product is functionally equivalent to the atami1 gene of arabidopsis thaliana and the aux2 gene of agrobacterium rhizogenes, was isolated from by-2 cells. overexpression of the ntami1 gene allowed by-2 cells to proliferate at lower concentrations of iam, whereas suppression of the ntami1 gene by rna interference (rnai) caused severe gr ...200919121311
transformed hairy roots of arachis hypogea: a tool for studying root nodule symbiosis in a non-infection thread legume of the aeschynomeneae tribe.arachis hypogea is a non-"infection thread" (it) legume where rhizobial entry or dissemination in the nodules never involves it. rhizobia invade through epidermal "cracks" and directly access the cortical cells to develop the characteristic aeschynomenoid nodules. for investigating these nonclassical nodulation features in arachis spp., we developed an efficient procedure for agrobacterium rhizogenes r1000-mediated transformation of this plant. in this study, we optimized the induction of hairy ...200919132866
agrobacterium rhizogenes-mediated transformation and regeneration of the apocynum venetum.a system for the agrobacterium rhizogenes-mediated transformation and plant regeneration of a. venetum has been developed. the highest transformation frequency was 100%, achieved by using strain lba9402 with root explants. the highest density of hairy roots reached 22 when root explants transformed by r1000 cultured in the dark. adventitious shoots were obtained from profusely branched, fast-growing (type pbf) hairy roots, and the adventitious shoot induction frequency was 20%. regenerated shoot ...200819149183
effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the arabidopsis thaliana atsuc2 sucrose-proton symporter gene.atsuc2 (at1g22710) from arabidopsis thaliana encodes a phloem-localized sucrose/proton symporter required for efficient photoassimilate transport from source tissues to sink tissues. atsuc2 plays a key role in coordinating the demands of sink tissues with the output capacity of source leaves, and in maintaining phloem hydrostatic pressure during changes in plant-water balance. expression and activity are regulated, both positively and negatively, by developmental (sink to source transition) and ...200919154603
construction of disarmed ti plasmids transferable between escherichia coli and agrobacterium species.agrobacterium-mediated plant transformation has been used widely, but there are plants that are recalcitrant to this type of transformation. this transformation method uses bacterial strains harboring a modified tumor-inducing (ti) plasmid that lacks the transfer dna (t-dna) region (disarmed ti plasmid). it is desirable to develop strains that can broaden the host range. a large number of agrobacterium strains have not been tested yet to determine whether they can be used in transformation. in o ...200919181833
transformation of nasturtium officinale, barbarea verna and arabis caucasica for hairy roots and glucosinolate-myrosinase system production.hairy roots of nasturtium officinale, barbarea verna and arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with agrobacterium rhizogenes. hairy roots of n. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g(-1) dw). b. verna and a. caucasica hairy roots produced gluconasturtiin (max. 41 mg g(-1) dw) and methionine-derived glucoiberverin (max. 32 mg g(-1) dw), respectively. treatment of the roots with ami ...200919229477
anthraquinones from natural and transformed roots of plocama pendula.five new and 15 known anthraquinones have been isolated from plocama pendula root cultures transformed by agrobacterium rhizogenes. the new anthraquinones were named balonone, balonone methyl ether, and plocamanones a, b, and c. moreover, lucidin dimethyl ether was obtained for the first time as a natural compound. on the other hand, a study of p. pendula natural roots afforded another two new anthraquinones, i.e., plocamanone d and chionone. the anthraquinone isozyganein dimethyl ether has been ...200919235160
gras proteins form a dna binding complex to induce gene expression during nodulation signaling in medicago truncatula.the symbiotic association of legumes with rhizobia involves bacterially derived nod factor, which is sufficient to activate the formation of nodules on the roots of the host plant. perception of nod factor by root hair cells induces calcium oscillations that are a component of the nod factor signal transduction pathway. perception of the calcium oscillations is a function of a calcium- and calmodulin-dependent protein kinase, and this activates nodulation gene expression via two gras domain tran ...200919252081
enhanced resveratrol accumulation in rolb transgenic cultures of vitis amurensis correlates with unusual changes in cdpk gene expression.it has been established that transformation of vitis amurensis callus culture with the plant oncogene rolb of agrobacterium rhizogenes results in a high level of resveratrol production in the transformed culture. in the present report, we investigated two rolb transgenic v. amurensis cell cultures with different levels of rolb expression and resveratrol production. we examined whether the calcium ion flux and later steps of the calcium-mediated signal transduction pathway play a role in resverat ...200919285358
reproduction of meloidogyne javanica on plant roots genetically transformed by agrobacterium rhizogenes.reproduction of meloidogyne javanica was compared on several agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. m. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and south australian early dwarf red tomato than on bindweed, tropic tomato, lima bean, or carrot. roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. numbers of females produced in c ...198819290260
transport of the nematicide oxamyl in roots transformed with agrobacterium rhizogenes.infection of roots transformed with agrobacterium rhizogenes by meloidogyne incognita and heterodera schachtii second-stage juveniles was established in bicompartmental petri dishes. one compartment contained the murashige and skoog agar medium and the nematicide oxamyl, and the other compartment contained water agar. transformed roots of carrot, tomato, alfalfa, cowpea, rape, and sugarbeet were placed in the nutrient compartment and grew over the barrier that divided the petri dishes and into t ...198819290298
grapevine mate-type proteins act as vacuolar h+-dependent acylated anthocyanin transporters.in grapevine (vitis vinifera), anthocyanins are responsible for most of the red, blue, and purple pigmentation found in the skin of berries. in cells, anthocyanins are synthesized in the cytoplasm and accumulated into the vacuole. however, little is known about the transport of these compounds through the tonoplast. recently, the sequencing of the grapevine genome allowed us to identify genes encoding proteins with high sequence similarity to the multidrug and toxic extrusion (mate) family. amon ...200919297587
inhibition of snake venoms and phospholipases a(2) by extracts from native and genetically modified eclipta alba: isolation of active coumestans.we genetically modified eclipta alba using agrobacterium rhizogenes lba 9402, with the aim of producing secondary metabolites with pharmacological properties against phospholipase a(2) and the myotoxic activities of snake venom. extracts from in natura aerial parts and roots, both native and genetically modified (in vitro), were prepared and analysed by high-performance liquid chromatography. in natura materials showed the coumestan wedelolactone at higher concentration in the aerial parts, whil ...200919320636
phytoremediation of small organic contaminants using transgenic plants.the efficacy of transgenic plants in the phytoremediation of small organic contaminants has been investigated. two principal strategies have been pursued (1) the manipulation of phase i metabolic activity to enhance in planta degradation rates, or to impart novel metabolic activity, and (2) the enhanced secretion of reactive enzymes from roots leading to accelerated ex planta degradation of organic contaminants. a pair of dehalogenase genes from xanthobacter autotrophicus was expressed in tobacc ...200919342219
protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots.sedentary endoparasitic nematodes cause extensive damage to a large number of ornamental plants and food crops, with estimated economical losses over 100 billion us$ worldwide. various efforts have put forth in order to minimize nematode damage, which typically involve the use of nematicides that have high cost and enhanced toxicity to humans and the environment. additionally, different strategies have been applied in order to develop genetically modified plants with improved nematode resistance ...200919428757
agrobacterium in the genomics age. 200919439569
novel toxin-antitoxin system composed of serine protease and aaa-atpase homologues determines the high level of stability and incompatibility of the tumor-inducing plasmid ptic58.stability of plant tumor-inducing (ti) plasmids differs among strains. a high level of stability prevents basic and applied studies including the development of useful strains. the nopaline type ti plasmid ptic58 significantly reduces the transconjugant efficiency for incoming incompatible plasmids relative to the other type, such as octopine-type plasmids. in this study we identified a region that increases the incompatibility and stability of the plasmid. this region was located on a 4.3-kbp s ...200919447904
the fast technique: a simplified agrobacterium-based transformation method for transient gene expression analysis in seedlings of arabidopsis and other plant species.abstract:200919457242
hchl expression in hairy roots of beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (phba) glucose ester, and linkage of phba into cell walls.as part of a study to explore the potential for new or modified bio-product formation, beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. the hchl gene, encoding p-hydroxycinnamoyl-coa hydratase/lyase, was introduced into b. vulgaris under the control of a camv 35s promoter, using agrobacterium rhizogenes lba 9402. hairy root clones expressing the hchl gene, together with non-expressing clones, were analysed a ...200919457657
rolc strawberry plant adaptability, productivity, and tolerance to soil-borne disease and mycorrhizal interactions.the potential to improve strawberry cultivation was assessed regarding the use the rolc genes from agrobacterium rhizogenes that can confer higher levels of free cytokinins. strawberry (cv. calypso) rolc lines were produced by genetic transformation of agrobacterium tumefaciens. yield and fruit quality of the control and transgenic lines were measured under open-field conditions. the effects of the transgenic rolc lines depended on gene copy number: rolc lines with one (line a) or two gene (line ...200919466576
calcium spiking patterns and the role of the calcium/calmodulin-dependent kinase ccamk in lateral root base nodulation of sesbania rostrata.nodulation factor (nf) signal transduction in the legume-rhizobium symbiosis involves calcium oscillations that are instrumental in eliciting nodulation. to date, ca2+ spiking has been studied exclusively in the intracellular bacterial invasion of growing root hairs in zone i. this mechanism is not the only one by which rhizobia gain entry into their hosts; the tropical legume sesbania rostrata can be invaded intercellularly by rhizobia at cracks caused by lateral root emergence, and this proces ...200919470588
callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants.we analyzed the impact of ethylene and auxin disturbances on callus, shoots and agrobacterium rhizogenes-induced hairy root formation in tomato (solanum lycopersicum l.). the auxin low-sensitivity dgt mutation showed little hairy root initiation, whereas the ethylene low-sensitivity nr mutation did not differ from the control micro-tom cultivar. micro-tom and dgt hairy roots containing auxin sensitivity/biosynthesis rol and aux genes formed prominent callus onto media supplemented with cytokinin ...200919484241
enhanced flavonoid production in hairy root cultures of glycyrrhiza uralensis fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment.economically important compounds, such as licorice flavonoids, are present in insufficient amounts in the hairy roots. to overcome this problem, we took the transgenic approach combined with the elicitation technique to increase the flavonoid production. the glycyrrhiza uralensis fisch cdna encoding chalcone isomerase gene (chi) was over-expressed in hairy roots of g. uralensis fisch mediated by the disarmed agrobacterium rhizogenes a4. stable genetic transformation was confirmed by southern blo ...200919504262
[somatic embryogenesis in the panax ginseng cell culture induced by the rolc oncogene is associated with increased expression of wus and serk genes].the embryogenic cell culture 2c3 was previously obtained by the transfer of the rolc gene from agrobacterium rhizogenes into ginseng callus cells. it was found by us that the expression of serk and wus genes in the embryogenic culture 2c3 is increased. these genes are known to be responsible for the initial stimulus to the development of embryogenesis in plants. taking into consideration earlier data, we suppose that the development of somatic embryos in the ginseng cell culture 2c3 is associate ...200919507704
agrobacterium aiming for the host chromatin: host and bacterial proteins involved in interactions between t-dna and plant nucleosomes.agrobacterium genetically transforms its hosts by transferring a segment of dna (t-dna) into the host cell and integrating it into the host genome. integration requires a close interaction between t-dna, which is packaged into a nucleoprotein complex (t-complex) by bacterial virulence (vir) proteins, and the host chromatin. this interaction is facilitated by the host protein vip 1, which binds both to the major protein component of the t-complex, vire2, and to the core histones. recently, vip1 h ...200919513263
catapol production in chinese foxglove (rehmannia glutinosa libos.) hairy roots transformed with agrobacterium rhizogenes atcc15834.hairy root clones of rehmannia glutinosa were established via transformation with agrobacterium rhizogenes atcc15834. to optimize the culturing conditions for both root growth and catalpol production, the effects of various combinations of seven basal media, ph, and carbon sources were examined in the dark. the fastest root growth was obtained in an sh medium containing 4% sucrose, ph 5.8. the highest catalpol content (0.54% dry weight) was achieved in a wpm medium supplemented with 4% sucrose, ...200919521851
hepatoprotective triterpenes from hairy root cultures of ocimum basilicum l.six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of ocimum basilicum l. (lamiaceae). these cultures were obtained by genetic transformation using agrobacterium rhizogenes. the extract as well as the isolated compounds were evaluated for their hepatoprotective activity by measuring their effect on the oxidative stress status of liver, induced by carbon tetrachlor ...200919526713
agrobacterium rhizogenes-mediated transformation of superroot-derived lotus corniculatus plants: a valuable tool for functional genomics.transgenic approaches provide a powerful tool for gene function investigations in plants. however, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. superroot of lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. recently, a technique to obtain transgenic l. corniculatus plants from superroot-derived leaves through a. tu ...200919555486
composite medicago truncatula plants harbouring agrobacterium rhizogenes-transformed roots reveal normal mycorrhization by glomus intraradices.composite plants consisting of a wild-type shoot and a transgenic root are frequently used for functional genomics in legume research. although transformation of roots using agrobacterium rhizogenes leads to morphologically normal roots, the question arises as to whether such roots interact with arbuscular mycorrhizal (am) fungi in the same way as wild-type roots. to address this question, roots transformed with a vector containing the fluorescence marker dsred were used to analyse am in terms o ...200919574251
phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance.agrobacterium rhizogenes-induced cotton (gossypium hirsutum l.) hairy roots were evaluated as a model system for studying molecular cotton-nematode interactions. hairy root cultures were developed from the root-knot nematode (rkn) (meloidogyne incognita [kofoid and white] chitwood, race 3)-resistant breeding line m315 and from the reniform nematode (rn) (rotylenchulus reniformis linford & oliveira)-resistant accession gb713 (g. barbadense l.) and compared to a nematode-susceptible culture derive ...200919578854
(homo)glutathione depletion modulates host gene expression during the symbiotic interaction between medicago truncatula and sinorhizobium meliloti.under nitrogen-limiting conditions, legumes interact with symbiotic rhizobia to produce nitrogen-fixing root nodules. we have previously shown that glutathione and homoglutathione [(h)gsh] deficiencies impaired medicago truncatula symbiosis efficiency, showing the importance of the low m(r) thiols during the nodulation process in the model legume m. truncatula. in this study, the plant transcriptomic response to sinorhizobium meliloti infection under (h)gsh depletion was investigated using cdna- ...200919587096
a nuclear-targeted cameleon demonstrates intranuclear ca2+ spiking in medicago truncatula root hairs in response to rhizobial nodulation factors.lipochitooligosaccharide nodulation factors (nfs) secreted by endosymbiotic nitrogen-fixing rhizobia trigger ca(2+) spiking in the cytoplasmic perinuclear region of host legume root hairs. to determine whether nfs also elicit ca(2+) responses within the plant cell nucleus we have made use of a nucleoplasmin-tagged cameleon (nupyc2.1). confocal microscopy using this nuclear-specific calcium reporter has revealed sustained and regular ca(2+) spiking within the nuclear compartment of medicago trunc ...200919700563
mutations that disrupt either the pqq or the gdh gene of rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall.rahnella aquatilis hx2, a biocontrol agent for grapevine crown gall caused by agrobacterium vitis, produces an antibacterial substance that inhibits the growth of a. vitis in vitro. in this study, we show that mh15 and mh16, two tn5-induced mutants of hx2, have lost their abilities to inhibit a. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single tn5 insertions. they are also impaired in producing pyrroloquinolin ...200919734331
medicago n2-fixing symbiosomes acquire the endocytic identity marker rab7 but delay the acquisition of vacuolar identity.rhizobium bacteria form n(2)-fixing organelles, called symbiosomes, inside the cells of legume root nodules. the bacteria are generally thought to enter the cells via an endocytosis-like process. to examine this, we studied the identity of symbiosomes in relation to the endocytic pathway. we show that in medicago truncatula, the small gtpases rab5 and rab7 are endosomal membrane identity markers, marking different (partly overlapping) endosome populations. although symbiosome formation is consid ...200919734435
a small gtpase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association.legume plants are able to establish a symbiotic relationship with soil bacteria from the genus rhizobium, leading to the formation of nitrogen-fixing root nodules. successful nodulation requires both the formation of infection threads (its) in the root epidermis and the activation of cell division in the cortex to form the nodule primordium. this study describes the characterization of raba2, a common bean (phaseolus vulgaris) cdna previously isolated as differentially expressed in root hairs in ...200919749154
efficient production of gossypol from hairy root cultures of cotton (gossypium hirsutum l.).a protocol for induction and establishment of agrobacterium rhizogenes mediated hairy root culture of gossypium hirsutum was developed through infection with the a4 strain and co-cultivation on hormone-free semi-solid ms medium with b5 vitamins. it resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. the transformation of hairy roots was established by pcr amplification of rol b and rol c genes of the ri plasmid. all root lines expressed gossypol, although di ...200919751178
surface motility and associated surfactant production in agrobacterium vitis.agrobacterium vitis is the causal agent of crown gall of grapevine. surface motility (swarming), an important mechanism for bacterial colonization of new environments and a previously unknown behaviour of ag. vitis, was demonstrated.200919780963
adventitious rooting is enhanced by methyl jasmonate in tobacco thin cell layers.adventitious roots (ars) are induced by auxins. jasmonic acid (ja) and methyl jasmonate (meja) are also plant growth regulators with many effects on development, but their role on ars needs investigation. to this aim, we analyzed ar formation in tobacco thin cell layers (tcls) cultured with 0.01-10 microm meja, either under root-inductive conditions, i.e., on medium containing 10 microm indole-3-butyric acid (iba) and 0.1 microm kinetin, or without hormones. the explants were excised from the cu ...200919885676
[culture of transgenic glycyrrhiza uralensis hairy root with licorice squalene synthase (sqs) gene].a squalene synthase gene cloned (gusqs1, accession number in genbank database: am182329) from glycyrrhiza uralensis was transferred into g. uralensis via agrobacterium rhizogenes a4 for investigating biosynthesis pathway and enhancing synthesis of glycyrrhizic acid (ga). hypocotyl explants from g. uralensis were infected with a. rhizogenes a4 containing gusqs1 gene to induce the hairy roots. the hairy root lines established were selected in medium containing 0.8 mg x l(-1) phosphinothricin (ppt) ...200919894527
growth kinetics and ginsenosides production in transformed hairy roots of american ginseng--panax quinquefolium l.a thin, profusely branched, fast growing hairy root line of panax quinquefolium (american ginseng) was established by co-culturing epicotyl explants with a wild type strain of agrobacterium rhizogenes. the transformed roots grew by over 10-fold from the initial inoculum within 8 weeks. the crude ginsenosides content in the roots was about 0.2 g/g dry wt level up to the 10th week of culture. ginsenosides rb2, rd, re, rf and rg1 constituted 47-49% of the crude saponin fraction between 6 and 8 week ...201019898783
tropane alkaloid profiling of hydroponic datura innoxia mill. plants inoculated with agrobacterium rhizogenes.hydroponics has been shown as a possible way to produce high quality plant biomass with improved phytochemical levels. nevertheless, effects of plant biotic and abiotic environment can lead to drastic changes and plant growth conditions must be optimised.201019904728
developing a systems biology approach to study disease progression caused by heterodera glycines in glycine max. 200719936075
biological diversity of prokaryotic type iv secretion systems.type iv secretion systems (t4ss) translocate dna and protein substrates across prokaryotic cell envelopes generally by a mechanism requiring direct contact with a target cell. three types of t4ss have been described: (i) conjugation systems, operationally defined as machines that translocate dna substrates intercellularly by a contact-dependent process; (ii) effector translocator systems, functioning to deliver proteins or other macromolecules to eukaryotic target cells; and (iii) dna release/up ...200919946141
isoflavonoid production in a hairy roots culture of pueraria candollei.a hairy roots culture of pueraria candollei was established using agrobacterium rhizogenes atcc15834 and grown in half-strength murashige and skoog (ms) medium. the highest production of total isoflavonoids was found to be (36.48 +/- 4.09) mg/g dry wt [(3.39 +/- 0.20) mg/g dry wt puerarin, (29.91 +/- 3.74) mg/g dry wt daidzin, (1.65 +/- 0.09) mg/g dry wt genistin, (0.76 +/- 0.03) mg/g dry wt daidzein, and (0.76 +/- 0.03) mg/g dry wt genistein, respectively]. the total isoflavonoid content in hai ...200919957437
dicentrine production from a hairy roots culture of stephania suberosa.a hairy roots culture of stephania suberosa was established using agrobacterium rhizogenes atcc15834. the production of dicentrine was found to be (8.92 +/- 0.07) mg/g dry wt on day 35 of culture. effects of sucrose content, tyrosine, and medium strength on growth and dicentrine production of s. suberosa were investigated. 6% (w/v) sucrose was an optimum content for the growth and dicentrine accumulation in s. suberosa hairy roots. the utilization of a precursor from tyrosine feeding enhanced th ...200919957438
rapid in planta evaluation of root expressed transgenes in chimeric soybean plants.production of stable transgenic plants is one factor that limits rapid evaluation of tissue specific transgene expression. to hasten the assessment of transgenes in planta, we evaluated the use of chimeric soybean seedlings expressing transgenic products in roots. tap roots from four-day old seedlings (cultivars 'jack' and ks4704) were excised and hairy roots were induced from hypocotyls via agrobacterium rhizogenes-mediated transformation. inoculated hypocotyls were screened on a ms-based mediu ...201020012965
generation of backbone-free, low transgene copy plants by launching t-dna from the agrobacterium chromosome.in both applied and basic research, agrobacterium-mediated transformation is commonly used to introduce genes into plants. we investigated the effect of three agrobacterium tumefaciens strains and five transferred (t)-dna origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-t-dna portions of the t-dna-containing vector (backbone) into the genome of arabidopsis (arabidopsis thaliana) and maize (zea mays). launching t-dna from the pica ...201020023148
generation of backbone-free, low transgene copy plants by launching t-dna from the agrobacterium chromosome.in both applied and basic research, agrobacterium-mediated transformation is commonly used to introduce genes into plants. we investigated the effect of three agrobacterium tumefaciens strains and five transferred (t)-dna origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-t-dna portions of the t-dna-containing vector (backbone) into the genome of arabidopsis (arabidopsis thaliana) and maize (zea mays). launching t-dna from the pica ...201020023148
the receiver domain of hybrid histidine kinase vira: an enhancing factor for vir gene expression in agrobacterium tumefaciens.the plant pathogen agrobacterium tumefaciens expresses virulence (vir) genes in response to chemical signals found at the site of a plant wound. vira, a hybrid histidine kinase, and its cognate response regulator, virg, regulate vir gene expression. the receiver domain at the carboxyl end of vira has been described as an inhibitory element because its removal increased vir gene expression relative to that of full-length vira. however, experiments that characterized the receiver region as an inhi ...201020081031
bnyvv-derived dsrna confers resistance to rhizomania disease of sugar beet as evidenced by a novel transgenic hairy root approach.agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsrna from the beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. genetically engineered roots were similar in morphology to wild type roots but were characterized by a profound abundancy, rapid growth rate and, in some cases, plagiotropic development. upon challenge inoculation, seedlings show ...201020127510
a remorin protein interacts with symbiotic receptors and regulates bacterial infection.remorin proteins have been hypothesized to play important roles during cellular signal transduction processes. induction of some members of this multigene family has been reported during biotic interactions. however, no roles during host-bacteria interactions have been assigned to remorin proteins until now. we used root nodule symbiosis between medicago truncatula and sinorhizobium meliloti to study the roles of a remorin that is specifically induced during nodulation. here we show that this ol ...201020133878
bioinformatic and experimental survey of 14-3-3-binding sites.more than 200 phosphorylated 14-3-3-binding sites in the literature were analysed to define 14-3-3 specificities, identify relevant protein kinases, and give insights into how cellular 14-3-3/phosphoprotein networks work. mode i rxx(ps/pt)xp motifs dominate, although the +2 proline residue occurs in less than half, and lx(r/k)sx(ps/pt)xp is prominent in plant 14-3-3-binding sites. proline at +1 is rarely reported, and such motifs did not stand up to experimental reanalysis of human ndel1. instea ...201020141511
new insights into an old story: agrobacterium-induced tumour formation in plants by plant transformation.agrobacterium tumefaciens causes tumour formation in plants. plant signals induce in the bacteria the expression of a range of virulence (vir) proteins and the formation of a type iv secretion system (t4ss). on attachment to plant cells, a transfer dna (t-dna) and vir proteins are imported into the host cells through the bacterial t4ss. through interaction with a number of host proteins, the vir proteins suppress the host innate immune system and support the transfer, nuclear targeting, and inte ...201020150897
agrobacterium rhizogenes-mediated transformation of hypericum tomentosum l and hypericum tetrapterum fries.this is the first evidence on successful agrobacterium rhizogenes-mediated genetic transformation of two species from the genus hypericum, h. tomentosum l. and h. tetrapterum fries. hairy root cultures were induced from root segments of both hypericum species by two agropine wild-type strains of a. rhizogenes, atcc 15834 and a4. the transgenic character of the hairy root cultures was proved by pcr amplification of the rolabcd genes. in some h. tetrapterum transgenic lines aux genes were detected ...200920158159
induction of pyridine alkaloid formation in transformed root cultures of nicotiana tabacum.hairy root cultures of nicotiana tabacum were set up by excised root tips with agrobacterium rhizogenes. the successful transformation was confirmed by analyzing rolc and virc genes using polymerase chain reaction (pcr). hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. the transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids. ...200920158160
molecular analysis of scarecrow genes expressed in white lupin cluster roots.the scarecrow (scr) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent centre and differentiation of the endodermis. in response to phosphorus (p) deficiency, white lupin (lupinus albus l.) root surface area increases some 50-fold to 70-fold due to the development of cluster (proteoid) roots. previously it was reported that scr-like expressed sequence tags (ests) were expressed during early cluster root development. here the ...201020167612
purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom clitocybe sinopica.a novel antibacterial protein with a molecular mass of 44 kda has been isolated from dried fruiting bodies of the wild mushroom clitocybe sinopica. sodium dodecyl sulfate/polyacrylamide gel electrophoresis showed that the protein was composed of two subunits each with a molecular mass of 22 kda. its n-terminal amino-acid sequence, svqatvngdkml, has not been reported for other antimicrobial proteins. the purification protocol included ion exchange chromatography on deae-cellulose, cm-cellulose an ...201020198215
performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. in the present study, we prospectively compared maldi-tof ms to the conventional phenotypic method for the identification of routine isolates. colonies were analyzed by maldi-tof ms either by direct deposition on the target plate or after a formic acid- ...201020220166
effect of certain elicitors on production of pyrrolizidine alkaloids in hairy root cultures of echium rauwolfii.hairy root cultures of echium rauwolfii were obtained by infection of sterile apical shoots with agrobacterium rhizogenes. the linear increase in fresh weight was found to be parallel to the alkaloids production. the transformed cultures were exposed to different elicitors, such as methyl jasmonate (mj), quercetin and salicylic acid in order to increase their productivity. pyrrolizidine alkaloids were quantitatively determined by hplc. estimation of total alkaloids was achieved by peak area calc ...201020383945
biochemical characterization of gram-positive and gram-negative plant-associated bacteria with micro-raman spectroscopy.raman spectra of gram-positive and gram-negative plant bacteria have been measured with micro-raman spectrometers equipped with 785 and 514.5 nm lasers. the gram-positive bacteria microbacterium testaceum, paenibacillus validus, and clavibacter michiganensis subsp. michiganensis have strong carotenoid bands in the regions 1155-1157 cm(-1) and 1516-1522 cm(-1) that differentiate them from other tested gram-negative bacteria. in the raman spectrum of gram-positive bacteria bacillus megaterium exci ...201020412629
impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of psoralea corylifolia.transformed hairy roots of psoralea corylifolia were established by infection with agrobacterium rhizogenes lba 9402. the aim of this work was to elucidate the effects of media constituents on production of the phytoestrogenic isoflavones daidzein and genistein. a. rhizogenes strain lba 9402 harboring ri plasmid was used to transform stem segments of in vitro seedlings. the resultant hairy roots were confirmed by polymerase chain reaction (pcr) and exhibited ri t-dna. transformed hairy root clon ...201020428956
establishment of a transgenic hairy root system in wild and domesticated watermelon (citrullus lanatus) for studying root vigor under drought.root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. here we established an efficient protocol for the genetic manipulation of two varieties of watermelon plants: a desert-grown wild watermelon that shows vigorous root growth under drought, and a domesticated cultivar showing retarda ...201020445980
agrobacterium rhizogenes-mediated transformation of a high oil-producing filamentous fungus umbelopsis isabellina.the agrobacterium rhizogenes-mediated transformation procedure was developed by using the hygromycin b phosphotransferase gene (hph) as a selective marker for the oil-producing fungus umbelopsis isabellina. different conditions were combined to increase the transformation efficiency. the highest efficiency was obtained by using a. rhizogenes strain r105 and a vector with zygomycete promoter. southern blot analysis demonstrated that 71% of transformants contained random integrations of t-dna sequ ...201020453313
misexpression of mir482, mir1512, and mir1515 increases soybean nodulation.micrornas (mirnas) are important regulators of plant growth and development. previously, we identified a group of conserved and novel mirna families from soybean (glycine max) roots. many of these mirnas are specifically induced during soybean-bradyrhizobium japonicum interactions. here, we examined the gene expression levels of six families of novel mirnas and investigated their functions in nodule development. we used northern-blot analyses to study the tissue specificity and time course of mi ...201020508137
Displaying items 601 - 700 of 1619