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expression, purification, crystallization and preliminary x-ray analysis of pseudomonas aeruginosa algx.algx is a periplasmic protein required for the production of the exopolysaccharide alginate in pseudomonas sp. and azotobacter vinelandii. algx has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. the crystals grew as flat plates with unit-cell parameters a = 46.4, b = 120.6, c = 86.9 a, beta = 95.7 degrees . the crystals exhibited the symmetry of space group p2(1) and diffracted to a minimum d-s ...201020445266
kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from chloroflexus aurantiacus j-10-fl: implications for functional and structural diversity in the tautomerase superfamily .4-oxalocrotonate tautomerase (4-ot) isozymes play prominent roles in the bacterial utilization of aromatic hydrocarbons as sole carbon sources. these enzymes catalyze the conversion of 2-hydroxy-2,4-hexadienedioate (or 2-hydroxymuconate) to 2-oxo-3-hexenedioate, where pro-1 functions as a general base and shuttles a proton from the 2-hydroxyl group of the substrate to the c-5 position of the product. 4-ot, a homohexamer from pseudomonas putida mt-2, is the most extensively studied 4-ot isozyme a ...201020465238
incidence, relevance and response for ralsfonia respiratory infections.cases of ralstonia colonization/infection occasionally reported by hospitals has generated increased interest in an organism previously little known to most clinicians. our goal was to determine the incidence of respiratory colonizations and infections involving ralstonia and the association of mechanical ventilation (limited to reports on respiratory-related occurrences in the u.s.a.) and propose a decision chart to assist response.201020499534
thioesterases: a new perspective based on their primary and tertiary structures.thioesterases (tes) are classified into ec 3.1.2.1 through ec 3.1.2.27 based on their activities on different substrates, with many remaining unclassified (ec 3.1.2.-). analysis of primary and tertiary structures of known tes casts a new light on this enzyme group. we used strong primary sequence conservation based on experimentally proved proteins as the main criterion, followed by verification with tertiary structure superpositions, mechanisms, and catalytic residue positions, to accurately de ...201020506386
molecular characterization of a class i p450 electron transfer system from novosphingobium aromaticivorans dsm12444.cytochrome p450 (cyp) enzymes of the cyp101 and cyp111 families from the oligotrophic bacterium novosphingobium aromaticivorans dsm12444 are heme monooxygenases that receive electrons from nadh via arx, a [2fe-2s] ferredoxin, and arr, a ferredoxin reductase. these systems show fast nadh turnovers (k(cat) = 39-91 s(-1)) that are efficiently coupled to product formation. the three-dimensional structures of arr, arx, and cyp101d1, which form a physiological class i p450 electron transfer chain, hav ...201020576606
identification and characterization of a novel estrogenic ligand actinopolymorphol a.xenoestrogenic compounds are abundant in the modern environment including phytoestrogens from plants, chemical by-products from industry, and secondary metabolites from microbes; all can profoundly affect human health. consequently mechanism-based screens are urgently needed to improve the rate at which the xenoestrogens are discovered. estrogen receptor (er) dimerization is required for target gene transcription. the three er dimer pairs (eralpha/alpha homodimers, erbeta/beta homodimers, and er ...201020599778
innate immunology of bovine respiratory disease.there are a variety of factors and conditions that predispose cattle to pneumonia. cattle have anatomic and cellular differences from humans and other species and are managed in groups/herds, all of which can increase susceptibility to microbial pathogens. this article highlights the basic innate immune response of the respiratory tract and newer developments in the understanding of adaptive immune responses of the bovine respiratory tract, placing special emphasis on features unique to cattle.201020619180
stya1 and stya2b from rhodococcus opacus 1cp: a multifunctional styrene monooxygenase system.two-component flavoprotein monooxygenases are emerging biocatalysts that generally consist of a monooxygenase and a reductase component. here we show that rhodococcus opacus 1cp encodes a multifunctional enantioselective flavoprotein monooxygenase system composed of a single styrene monooxygenase (smo) (stya1) and another styrene monooxygenase fused to an nadh-flavin oxidoreductase (stya2b). stya1 and stya2b convert styrene and chemical analogues to the corresponding epoxides at the expense of f ...201020675468
scarless and sequential gene modification in pseudomonas using pcr product flanked by short homology regions.the lambda red recombination system has been used to inactivate chromosomal genes in various bacteria and fungi. the procedure consists of electroporating a polymerase chain reaction (pcr) fragment containing antibiotic cassette flanked by homology regions to the target locus into a strain that can express the lambda red proteins (gam, bet, exo).201020682065
moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity. 201020730219
partial purification and characterization of the mode of action of enterocin s37: a bacteriocin produced by enterococcus faecalis s37 isolated from poultry feces.the aim of this research was to purify and characterize the mode of action of enterocin s37, a bacteriocin produced by enterococcus faecalis s37, a strain recently isolated from the chicken feces. enterocin s37 has a molecular weight comprised between 4 and 5 kda. it remained active after 1 h at 80(o)c and at ph values ranging from 4.0 to 9.0. furthermore, cell-free supernatant of enterococcus faecalis s37 and purified enterocin s37 were active against gram-positive bacteria including listeria m ...201020811593
isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ...201020874394
lipoic acid synthesis: a new family of octanoyltransferases generally annotated as lipoate protein ligases.bacillus subtilis lacks a recognizable homologue of the lipb octanoyltransferase, an enzyme essential for lipoic acid synthesis in escherichia coli. lipb transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-lipb intermediate. the octanoylated dehydrogenase is then converted to the enzymatically active lipoylated species by insertion of two sulfur atoms into the octanoyl moiety by the s-adenosyl-l-me ...201020882995
activity and interactions of antibiotic and phytochemical combinations against pseudomonas aeruginosa in vitro.in this study the in vitro activities of seven antibiotics (ciprofloxacin, ceftazidime, tetracycline, trimethoprim, sulfamethoxazole, polymyxin b and piperacillin) and six phytochemicals (protocatechuic acid, gallic acid, ellagic acid, rutin, berberine and myricetin) against five p. aeruginosa isolates, alone and in combination are evaluated. all the phytochemicals under investigation demonstrate potential inhibitory activity against p. aeruginosa. the combinations of sulfamethoxazole plus proto ...201020941374
expression of an organic solvent stable lipase from staphylococcus epidermidis at2.an organic solvent tolerant lipase gene from staphylococcus epidermidis at2 was successfully cloned and expressed with ptrchis2 in e. coli top10. sequence analysis revealed an open reading frame (orf) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. the polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. expression of at2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mm ipt ...201020957088
keratitis after implantation of intrastromal corneal rings with spontaneous extrusion of the segment.purpose: to report a case of bacterial keratitis in a patient with a history of intrastromal corneal ring segments (intacs®) implantation to correct keratoconus. methods: the patient's history, clinical presentation, pathological analysis and therapeutic management were reviewed. results: a 36-year-old-man was referred to our department due to decreased vision and intense pain in his left eye, 40 days after intacs® implantation for keratoconus. slit-lamp examination revealed epithelial defects a ...201021060771
thny is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation gene expression.previous genetic studies in sphingomonas macrogolitabida strain tfa have established that expression of genes involved in tetralin biodegradation (thn genes) requires the function of the lysr type activator thnr and also thny. sequence comparison indicated that thny is homologous to bacterial oxygenase-coupled nad(p)h-dependent ferredoxin reductases. however, thny showed substitutions in highly conserved positions of the pyridine nucleotide binding domain of these ferredoxin reductases. thny exp ...201021068394
thny is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation gene expression.previous genetic studies in sphingomonas macrogolitabida strain tfa have established that expression of genes involved in tetralin biodegradation (thn genes) requires the function of the lysr type activator thnr and also thny. sequence comparison indicated that thny is homologous to bacterial oxygenase-coupled nad(p)h-dependent ferredoxin reductases. however, thny showed substitutions in highly conserved positions of the pyridine nucleotide binding domain of these ferredoxin reductases. thny exp ...201021068394
structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution.the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ...201021073881
structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution.the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ...201021073881
hydrogenosome-localization of arginine deiminase in trichomonas vaginalis.the arginine dihydrolase (adh) pathway has an analogous function to the urea cycle in mitochondria-containing cells, by removing nitrogen from amino acids and generating atp. subcellular localization of the adh pathway enzymes in trichomonas vaginalis revealed that arginine deiminase (adi) localizes to the hydrogenosome, a mitochondrion-like organelle of anaerobic protists. however the other enzymes of the adh pathway, ornithine carbamyltransferase and carbamate kinase localize to the cytosol. t ...201021074581
hydrogenosome-localization of arginine deiminase in trichomonas vaginalis.the arginine dihydrolase (adh) pathway has an analogous function to the urea cycle in mitochondria-containing cells, by removing nitrogen from amino acids and generating atp. subcellular localization of the adh pathway enzymes in trichomonas vaginalis revealed that arginine deiminase (adi) localizes to the hydrogenosome, a mitochondrion-like organelle of anaerobic protists. however the other enzymes of the adh pathway, ornithine carbamyltransferase and carbamate kinase localize to the cytosol. t ...201021074581
giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosine ligase-like member and deglycylated by two metallocarboxypeptidases.the flagellated protozoan giardia duodenalis is a parasite of the upper part of the small intestine of mammals, including humans, and an interesting biological model. giardia harbors a single 14-3-3 isoform, a multifunctional protein family, that is modified at the c terminus by polyglycylation, an unusual post-translational modification consisting of the covalent addition of one or multiple glycines on the γ-carboxyl groups of specific glutamic acids. polyglycylation affects the intracellular l ...201021135098
giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosine ligase-like member and deglycylated by two metallocarboxypeptidases.the flagellated protozoan giardia duodenalis is a parasite of the upper part of the small intestine of mammals, including humans, and an interesting biological model. giardia harbors a single 14-3-3 isoform, a multifunctional protein family, that is modified at the c terminus by polyglycylation, an unusual post-translational modification consisting of the covalent addition of one or multiple glycines on the γ-carboxyl groups of specific glutamic acids. polyglycylation affects the intracellular l ...201021135098
nature of the reaction intermediates in the flavin adenine dinucleotide-dependent epoxidation mechanism of styrene monooxygenase.styrene monooxygenase (smo) is a two-component flavoenzyme composed of an nadh-specific flavin reductase (smob) and fad-specific styrene epoxidase (nsmoa). nsmoa binds tightly to reduced fad and catalyzes the stereospecific addition of one atom of molecular oxygen to the vinyl side chain of styrene in the enantioselective synthesis of s-styrene oxide. in this mechanism, molecular oxygen first reacts with nsmoa(fad(red)) to yield an fad c(4a)-peroxide intermediate. this species is nonfluorescent ...201021166448
nature of the reaction intermediates in the flavin adenine dinucleotide-dependent epoxidation mechanism of styrene monooxygenase.styrene monooxygenase (smo) is a two-component flavoenzyme composed of an nadh-specific flavin reductase (smob) and fad-specific styrene epoxidase (nsmoa). nsmoa binds tightly to reduced fad and catalyzes the stereospecific addition of one atom of molecular oxygen to the vinyl side chain of styrene in the enantioselective synthesis of s-styrene oxide. in this mechanism, molecular oxygen first reacts with nsmoa(fad(red)) to yield an fad c(4a)-peroxide intermediate. this species is nonfluorescent ...201021166448
screening of ethanol, petroleum ether and chloroform extracts of medicinal plants, lawsonia inermis l. and mimosa pudica l. for antibacterial activity.organic extracts (ethanol, petroleum ether and chloroform) of two medicinal plants lawsonia inermis l. and mimosa pudica l. were proven for antibacterial properties against 15 gram-positive and gram-negative human pathogenic bacteria. among the three types of extracts tested, ethanol extract was found to possess maximum antibacterial activity. the diameter of the zone of inhibition of bacterial growth showed that gram-negative bacteria are more sensitive than gram-positive bacteria to plant extr ...201021188055
real-time solvent tolerance analysis of pseudomonas sp. strain vlb120{delta}c catalytic biofilms.biofilms are ubiquitous surface-associated microbial communities embedded in an extracellular polymeric (eps) matrix, which gives the biofilm structural integrity and strength. it is often reported that biofilm-grown cells exhibit enhanced tolerance toward adverse environmental stress conditions, and thus there has been a growing interest in recent years to use biofilms for biotechnological applications. we present a time- and locus-resolved, noninvasive, quantitative approach to study biofilm d ...201021193676
mechanistic studies of 1-aminocyclopropane-1-carboxylate deaminase: characterization of an unusual pyridoxal 5'-phosphate-dependent reaction.1-aminocyclopropane-1-carboxylic acid (acc) deaminase (accd) is a pyridoxal 5'-phosphate (plp)-dependent enzyme that cleaves the cyclopropane ring of acc, to give α-ketobutyric acid and ammonia as products. the cleavage of the c(α)-c(β) bond of an amino acid substrate is a rare event in plp-dependent enzyme catalysis. potential chemical mechanisms involving nucleophile- or acid-catalyzed cyclopropane ring opening have been proposed for the unusual transformation catalyzed by accd, but the actual ...201121244019
aldehyde oxidase carrying an unusual subunit structure from pseudomonas sp. mx-058.pseudomonas sp. mx-058 produces aldehyde oxidase catalysing glyoxal to glyoxylic acid. two aldehyde oxidases (f10 and f13) were purified to homogeneity from pseudomonas sp. mx-058. f10 and f13 had subunit structures, a heterotetramer and heteropentamer respectively. the n-terminal amino acid sequences of all subunits were highly homologous to amino acid sequences of the putative oxidoreductases of pseudomonas strains. all of these homologous oxidoreductases have a heterotrimer structure consisti ...200821261859
atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation.atrazine is an herbicide of the s-triazine family that is used primarily as a nitrogen source by degrading microorganisms. while many catabolic pathways for xenobiotics are subjected to catabolic repression by preferential carbon sources, atrazine utilization is repressed in the presence of preferential nitrogen sources. this phenomenon appears to restrict atrazine elimination in nitrogen-fertilized soils by indigenous organisms or in bioaugmentation approaches. the mechanisms of nitrogen contro ...200921261912
differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx. 201121261963
clinical and antimicrobial efficacy of nitradine ™-based disinfecting cleaning tablets in complete denture wearers.this study evaluated the efficacy of nitradine ™-based disinfecting cleaning tablets for complete denture, in terms of denture biofilm removal and antimicrobial action.201021308285
engineering of a psychrophilic bacterium for the bioremediation of aromatic compounds.microbial degradation of aromatic hydrocarbons has been studied with the aim of developing applications for the removal of toxic compounds. efforts have been directed toward the genetic manipulation of mesophilic bacteria to improve their ability to degrade pollutants, even though many pollution problems occur in sea waters and in effluents of industrial processes which are characterized by low temperatures. from these considerations the idea of engineering a psychrophilic microorganism for the ...201021326928
multiple roles of component proteins in bacterial multicomponent monooxygenases: phenol hydroxylase and toluene/o-xylene monooxygenase from pseudomonas sp. ox1.phenol hydroxylase (ph) and toluene/o-xylene monooxygenase (tomo) from pseudomonas sp. ox1 require three or four protein components to activate dioxygen for the oxidation of aromatic substrates at a carboxylate-bridged diiron center. in this study, we investigated the influence of the hydroxylases, regulatory proteins, and electron-transfer components of these systems on substrate (phenol; nadh) consumption and product (catechol; h(2)o(2)) generation. single-turnover experiments revealed that on ...201121366224
purification of protease from pseudomonas thermaerum gw1 isolated from poultry waste site.an extracellular protease was purified from pseudomonas thermaerum gw1 a new strain identified by morphological, biochemical and 16s rdna sequencing. it was isolated from soil of poultry waste site at ghazipur near ghaziabad, delhi. the strain produces extra cellular protease in the culture media that was maintained at 37°c, 140 rpm. the media was harvested for protease after 48 hrs of incubation at 37°c in basal media supplemented with 1% casein. we report 6.08 fold purification of enzyme follo ...201021379398
the homogentisate and homoprotocatechuate central pathways are involved in 3- and 4-hydroxyphenylacetate degradation by burkholderia xenovorans lb400.genome characterization of the model pcb-degrading bacterium burkholderia xenovorans lb400 revealed the presence of eleven central pathways for aromatic compounds degradation, among them, the homogentisate and the homoprotocatechuate pathways. however, the functionality of these central pathways in strain lb400 has not been assessed and related peripheral pathways has not been described.201121423751
identification of outer membrane proteins from an antarctic bacterium pseudomonas syringae lz4w.subcellular fractionation of proteins is a preferred method of choice for detection and identification of proteins from complex mixtures such as bacterial cells. to characterize the membrane proteins of the antarctic bacterium pseudomonas syringae lz4w, the membrane fractions were prepared using three different methods, namely triton x-100 solubilization, sucrose density gradient, and carbonate extraction methods. the proteins were separated on one-dimensional polyacrylamide gels and analyzed us ...201121447709
intensive care management of liver transplanted patients.advances in pre-transplant treatment of cirrhosis-related organ dysfunction, intraoperative patient management, and improvements in the treatment of rejection and infections have made human liver transplantation an effective and valuable option for patients with end stage liver disease. however, many important factors, related both to an increasing "marginality" of the implanted graft and unexpected perioperative complications still make immediate post-operative care challenging and the early ou ...201121487537
evidence for polymicrobic flora translocating in peripheral blood of hiv-infected patients with poor immune response to antiretroviral therapy.in advanced hiv infection, the homeostatic balance between gastrointestinal indigenous bacteria and gut immunity fails and microbes are able to overcome the intestinal barrier and gain the systemic circulation. because microbial translocation is not fully controlled by antiviral therapy and is associated with inefficient cd4+ reconstitution, we investigated the profile of translocating bacteria in peripheral blood of 44 hiv-infected patients starting therapy with advanced cd4+ t-lymphopenia and ...201121494598
isolation of xylose isomerases by sequence- and function-based screening from a soil metagenome library.abstract: background: xylose isomerase (xi) catalyses the isomerization of xylose to xylulose in bacteria and some fungi. currently, only a limited number of xi genes have been functionally expressed in saccharomyces cerevisiae, the microorganism of choice for lignocellulosic ethanol production. the objective of the present study was to search for novel xi genes in the vastly diverse microbial habitat present in soil. as the exploitation of microbial diversity is impaired by the ability to culti ...201121545702
culture-independent analysis of bacterial fuel contamination provides insights into the level of concordance with the standard industry practice of aerobic cultivation.bacterial diversity in contaminated fuels has not been systematically investigated using cultivation-independent methods. the fuel industry relies on phenotypic cultivation-based contaminant identification, which may lack accuracy and neglect difficult-to-culture taxa. using industry practice aerobic cultivation, 16s rrna gene sequencing and strain genotyping, a collection of 152 unique contaminant isolates from 54 fuel samples was assembled, and demonstrated a dominance of pseudomonas (21%), bu ...201121602386
a comprehensive analysis of gene expression changes provoked by bacterial and fungal infection in c. elegans.while caenorhabditis elegans specifically responds to infection by the up-regulation of certain genes, distinct pathogens trigger the expression of a common set of genes. we applied new methods to conduct a comprehensive and comparative study of the transcriptional response of c. elegans to bacterial and fungal infection. using tiling arrays and/or rna-sequencing, we have characterized the genome-wide transcriptional changes that underlie the host's response to infection by three bacterial (serr ...201121602919
characterization of the structure and function of klebsiella pneumoniae allantoin racemase.the oxidative catabolism of uric acid produces 5-hydroxyisourate (hiu), which is further degraded to (s)-allantoin by two enzymes, hiu hydrolase and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase. the intermediates of the latter two reactions, hiu and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, are unstable in solution and decay nonstereospecifically to allantoin. in addition, nonenzymatic racemization of allantoin has been shown to occur at physiological ph. since the further br ...201121616082
molecular basis of glyphosate resistance-different approaches through protein engineering.glyphosate (n-phosphonomethyl-glycine) is the most widely used herbicide in the world: glyphosate-based formulations exhibit broad-spectrum herbicidal activity with minimal human and environmental toxicity. the extraordinary success of this simple, small molecule is mainly attributable to the high specificity of glyphosate for the plant enzyme enolpyruvyl shikimate-3-phosphate synthase in the shikimate pathway, leading to the biosynthesis of aromatic amino acids. starting in 1996, transgenic gly ...201121668647
genetic approach for the fast discovery of phenazine producing bacteria.a fast and efficient approach was established to identify bacteria possessing the potential to biosynthesize phenazines, which are of special interest regarding their antimicrobial activities. sequences of phze genes, which are part of the phenazine biosynthetic pathway, were used to design one universal primer system and to analyze the ability of bacteria to produce phenazine. diverse bacteria from different marine habitats and belonging to six major phylogenetic lines were investigated. bacter ...201121673888
kinetic, crystallographic, and mechanistic characterization of tomn: elucidation of a function for a 4-oxalocrotonate tautomerase homologue in the tomaymycin biosynthetic pathway.the biosynthesis of the c ring of the anti-tumor antibiotic agent, tomaymycin, is proposed to proceed through five enzyme-catalyzed steps from l-tyrosine. the genes encoding these enzymes have recently been cloned and their functions tentatively assigned, but there is limited biochemical evidence supporting the assignments of the last three steps. one enzyme, tomn, shows 58% pairwise sequence similarity with 4-oxalocrotonate tautomerase (4-ot), an enzyme found in a catabolic pathway for aromatic ...201121809870
mechanisms of nickel toxicity in microorganisms.nickel has long been known to be an important human toxicant, including having the ability to form carcinomas, but until recently nickel was believed to be an issue only to microorganisms living in nickel-rich serpentine soils or areas contaminated by industrial pollution. this assumption was overturned by the discovery of a nickel defense system (rcnr/rcna) found in microorganisms that live in a wide range of environmental niches, suggesting that nickel homeostasis is a general biological conce ...201121799955
xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.the xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. these large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. here, we show that some colonies of xenopus laevis are infected with three multi-drug-resistant bacteria: pseudomonas fluorescens, pseudomonas putida, and stenotrophomonas maltophilia. o ...201121788613
molecular identification of bacteria by total sequence screening: determining the cause of death in ancient human subjects.research of ancient pathogens in ancient human skeletons has been mainly carried out on the basis of one essential historical or archaeological observation, permitting specific pathogens to be targeted. detection of ancient human pathogens without such evidence is more difficult, since the quantity and quality of ancient dna, as well as the environmental bacteria potentially present in the sample, limit the analyses possible. using human lung tissue and/or teeth samples from burials in eastern s ...201121765907
microfluidic fluorescence in situ hybridization and flow cytometry (µflowfish).we describe an integrated microfluidic device (µflowfish) capable of performing 16s rrna fluorescence in situ hybridization (fish) followed by flow cytometric detection for identifying bacteria in natural microbial communities. the device was used for detection of species involved in bioremediation of cr(vi) and other metals in groundwater samples from a highly-contaminated environmental site (hanford, wa, usa). the µflowfish seamlessly integrates two components: a hybridization chamber formed b ...201121755095
novel antiphytopathogenic compound 2-heptyl-5-hexylfuran-3-carboxylic acid, produced by newly isolated pseudomonas sp. strain sjt25.pseudomonas sp. strain sjt25, which strongly antagonizes plant pathogens, was isolated from rice rhizosphere soil by a bioactivity-guided approach. a novel antiphytopathogenic compound was isolated from the fermentation broth of pseudomonas sp. sjt25 and identified as 2-heptyl-5-hexylfuran-3-carboxylic acid. this compound showed antimicrobial activities both in vitro and in vivo.201121742907
repression of the antifungal activity of pseudomonas sp. strain df41 by the stringent response.the stringent response (sr) enables bacteria to adapt to nutrient limitation through production of the nucleotides guanosine tetraphosphate and guanosine pentaphosphate, collectively known as (p)ppgpp. two enzymes are responsible for the intracellular pools of (p)ppgpp: rela acts as a synthetase, while spot can function as either a synthetase or a hydrolase. we investigated how the sr affects the ability of the biological control agent pseudomonas sp. strain df41 to inhibit the fungal pathogen s ...201121705548
kinetics of styrene biodegradation by pseudomonas sp. e-93486.the research into kinetics of styrene biodegradation by bacterial strain pseudomonas sp. e-93486 coming from vtt culture collection (finland) was presented in this work. microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and continuous cultures. batch experiments were conducted for initial concentration of styrene in the liquid phase changed in the range of 5-90-ágôçëm(-3). the haldane model was found to be the best to fit the kine ...201121833566
Development of a New Strategy for Production of Medium-Chain-Length Polyhydroxyalkanoates by Recombinant Escherichia coli via Inexpensive Non-Fatty Acid Feedstocks.Pseudomonas putida KT2440 is capable of producing medium-chain-length polyhydroxyalkanoates (MCL-PHAs) when grown on unrelated carbon sources during nutrient limitation. Transcription levels of genes putatively involved in PHA biosynthesis were assessed by quantitative real-time PCR (qRT-PCR) in P. putida grown on glycerol as a sole carbon source. The results showed that two genes, phaG and the PP0763 gene, were highly upregulated among genes potentially involved in the biosynthesis of MCL-PHAs ...201222101037
Investigation of the Catalytic Mechanism of the Hotdog-fold Enzyme Superfamily Pseudomonas sp. strain CBS3 4-Hydroxybenzoyl-CoA Thioesterase.The 4-hydroxybenzoyl-CoA (4-HB-CoA)1 thioesterase from Pseudomonas sp strain CBS3 catalyzes the final step of the 4-chlorobenzoate degradation pathway, which is the hydrolysis of 4-hydroxybenzoyl-CoA (4-HB-CoA) to coenzyme A (CoA) and 4-hydroxybenzoate. In previous work, X-ray structural analysis of the substrate-bound thioesterase provided evidence for the role of an active site Asp17 in nucleophilic catalysis (Thoden, J. B., Holden, H. M., Zhuang, Z., Dunaway-Mariano, D. (2002) X-ray crystallo ...201122208697
The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.Microorganisms, such as Pseudomonas putida, utilize specific physical properties of cellular membrane constituents, mainly glycerophospholipids, to (re-)adjust the membrane barrier to environmental stresses. Building a basis for membrane composition/function studies, we inventoried the glycerophospholipids of different Pseudomonas and challenged membranes of growing cells with n-butanol. Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid s ...201221895997
Lipoteichoic acid in Streptomyces hygroscopicus: structural model and immunomodulatory activities.Gram positive bacteria produce cell envelope macroamphiphile glycopolymers, i.e. lipoteichoic acids or lipoglycans, whose functions and biosynthesis are not yet fully understood. We report for the first time a detailed structure of lipoteichoic acid isolated from a Streptomyces species, i.e. Streptomyces hygroscopicus subsp. hygroscopicus NRRL 2387T. Chemical, MS and NMR analyses revealed a polyglycerolphosphate backbone substituted with a-glucosaminyl and a-N-acetyl-glucosaminyl residues but de ...201122028855
Analysis of Substrate Access to Active Sites in Bacterial Multicomponent Monooxygenase Hydroxylases: X-ray Crystal Structure of Xenon-Pressurized Phenol Hydroxylase from Pseudomonas sp. OX1.In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the a-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of dioxygen to the active site by crystallographic characterization of a xenon-pressurized sample of the hydroxylase component of phenol hydroxylase from Pseudomonas sp. OX1. Computat ...201122136180
Epidemiology of hospital-acquired urinary tract-related bloodstream infection at a university hospital.Little is known about the epidemiology of nosocomial urinary tract-related bloodstream infection. In a case series from an academic medical center, Enterococcus (28.7%) and Candida (19.6%) species were the predominant microorganisms, which suggests a potential shift from gram-negative microorganisms. A case-fatality rate of 32.8% highlights the severity of this condition.201122011543
[in process citation].le pronostic des brûlures est souvent tributaire de complications infectieuses. les objectifs de cette étude préliminaire rétrospective sont une évaluation de la flore microbienne et ses fluctuations selon la durée d'hospitalisation chez les patients du centre des brûlés adultes de annaba (algérie). ce travail porte sur 633 prélèvements microbiologiques positifs obtenus entre juin 2003 (inauguration du service) et décembre 2005. selon le site de prélèvement la répartition est: cutané (78%), hémo ...200821991102
[in process citation].le pseudomonasest un agent pathogène à l'origine d'infections nosocomiales graves dans les centres des brûlés. son opportunisme et sa virulence en font une préoccupation majeure. ce travail se propose d'évaluer la place de cette bactérie dans l'écologie bactérienne locale et d'en apprécier la sensibilité aux antibiotiques. cette étude rétrospective préliminaire porte sur la période de juin 2003 à décembre 2005. elle intéresse l'ensemble des prélèvements bactériologiques ayant pu être réalisés au ...200821991140
genomic analysis and temperature-dependent transcriptome profiles of the rhizosphere originating strain pseudomonas aeruginosa m18.our previously published reports have described an effective biocontrol agent named pseudomonas sp. m18 as its 16s rdna sequence and several regulator genes share homologous sequences with those of p. aeruginosa, but there are several unusual phenotypic features. this study aims to explore its strain specific genomic features and gene expression patterns at different temperatures.201121884571
the culturable intestinal microbiota of triploid and diploid juvenile atlantic salmon (salmo salar) - a comparison of composition and drug resistance.with the increased use of ploidy manipulation in aquaculture and fisheries management this investigation aimed to determine whether triploidy influences culturable intestinal microbiota composition and bacterial drug resistance in atlantic salmon (salmo salar). the results could provide answers to some of the physiological differences observed between triploid and diploid fish, especially in terms of fish health.201122094054
identification and engineering of the cytochalasin gene cluster from aspergillus clavatus nrrl 1.cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including cytochalasin e produced by aspergillus clavatus, which is a potent anti-angiogenic agent. here, we report the identification and characterization of the cytochalasin gene cluster from a. clavatus nrrl 1. as a producer of cytochalasin e and k, the genome of a. clavatus was analyzed and the ∼30 kb ccs gene cluster was identified based on the presence of a polyketide synthase-nonribosomal ...201121983160
prevalence of uropathogens in diabetic patients and their corresponding resistance pattern: results of a survey conducted at diagnostic centers in dhaka, bangladesh.urinary tract infection is the second most common clinical indication for empirical antibiotic treatment in primary and secondary health care settings. the incidence of diabetes mellitus throughout the world is increasing strikingly and in the long run, it has some major effects on the genitourinary system which makes diabetic patients more liable to urinary tract infection. this study is designed to reveal the distribution of uropathogens in diabetic patients according to age and sex, and corre ...201022043358
Influence of uranium on bacterial communities: a comparison of natural uranium-rich soils with controls.This study investigated the influence of uranium on the indigenous bacterial community structure in natural soils with high uranium content. Radioactive soil samples exhibiting 0.26% - 25.5% U in mass were analyzed and compared with nearby control soils containing trace uranium. EXAFS and XRD analyses of soils revealed the presence of U(VI) and uranium-phosphate mineral phases, identified as sabugalite and meta-autunite. A comparative analysis of bacterial community fingerprints using denaturing ...201121998695
Regulation of phenylacetic acid uptake is s54 dependent in Pseudomonas putida CA-3.Styrene is a toxic and potentially carcinogenic alkenylbenzene used extensively in the polymer processing industry. Significant quantities of contaminated liquid waste are generated annually as a consequence. However, styrene is not a true xenobiotic and microbial pathways for its aerobic assimilation, via an intermediate, phenylacetic acid, have been identified in a diverse range of environmental isolates. The potential for microbial bioremediation of styrene waste has received considerable res ...201121995721
molecular characterization and snp detection of cd14 gene of crossbred cattle.cd14 is an important molecule for innate immunity that can act against a wide range of pathogens. the present paper has characterized cd14 gene of crossbred (cb) cattle (bos indicus×bos taurus). cloning and sequence analysis of cd14 cdna revealed 1119 nucleotide long open reading frame encoding 373 amino acids protein and 20 amino acids signal peptide. cb cattle cd14 gene exhibited a high percentage of nucleotide identity (59.3-98.1%) with the corresponding mammalian homologs. cattle and buffalo ...201122132326
Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria.The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ...201122163022
a naturally associated rhizobacterium of arabidopsis thaliana induces a starvation-like transcriptional response while promoting growth.plant growth promotion by rhizobacteria is a known phenomenon but the underlying mechanisms are poorly understood. we searched for plant growth-promoting rhizobacteria that are naturally associated with arabidopsis thaliana to investigate the molecular mechanisms that are involved in plant growth-promotion. we isolated a pseudomonas bacterium (pseudomonas sp. g62) from roots of field-grown arabidopsis plants that has not been described previously and analyzed its effect on plant growth, gene exp ...201122216267
transferable integrons of gram-negative bacteria isolated from the gut of a wild boar in the buffer zone of a national park.the aim of this study was to determine the presence of integron-bearing gram-negative bacteria in the gut of a wild boar (sus scrofa l.) shot in the buffer zone of a national park. five gram-negative strains of escherichia coli, serratia odorifera, hafnia alvei and pseudomonas sp. were isolated. four of these strains had class 2 integrase (inti2), and one harbored class 1 integrase (inti1). the integron-positive strains were multiresistant, i.e., resistant to at least three unrelated antibiotics ...201122661922
metabolic characterization of cold active pseudomonas, arthrobacter, bacillus, and flavobacterium spp. from western himalayas.himalayan soils undergo dramatic temporal changes in their microclimatic properties. the soil habitats in the high altitude cold habitats of himalayas are little explored with respect to bacterial diversity and metabolic potentials of the bacterial species. soil habitat in western himalayas is dominated by the genera of pseudomonas, arthrobacter, bacillus, and flavobacterium. strains were found to be diverse in their metabolic potentials to utilize different carbon sources by growing them on med ...201122282631
a water-damaged home and health of occupants: a case study.a family of five and pet dog who rented a water-damaged home and developed multiple health problems. the home was analyzed for species of mold and bacteria. the diagnostics included mri for chronic sinusitis with ent and sinus surgery, and neurological testing for neurocognitive deficits. bulk samples from the home, tissue from the sinuses, urine, nasal secretions, placenta, umbilical cord, and breast milk were tested for the presence of trichothecenes, aflatoxins, and ochratoxin a. the family h ...201122220187
a water-damaged home and health of occupants: a case study.a family of five and pet dog who rented a water-damaged home and developed multiple health problems. the home was analyzed for species of mold and bacteria. the diagnostics included mri for chronic sinusitis with ent and sinus surgery, and neurological testing for neurocognitive deficits. bulk samples from the home, tissue from the sinuses, urine, nasal secretions, placenta, umbilical cord, and breast milk were tested for the presence of trichothecenes, aflatoxins, and ochratoxin a. the family h ...201122220187
production of indole acetic acid by pseudomonas sp.: effect of coinoculation with mesorhizobium sp. cicer on nodulation and plant growth of chickpea (cicer arietinum).pseudomonas isolates obtained from the rhizosphere of chickpea (cicer arietinum l.) and green gram (vigna radiata) were found to produce significant amount of indole acetic acid (iaa) when grown in a lb medium broth supplemented with l-tryptophan. seed bacterization of chickpea cultivar c235 with different pseudomonas isolates showed stunting effect on the development of root and shoot at 5 and 10 days of seedling growth except the strains mps79 and mps90 that showed stimulation of root growth, ...201123572992
biological control of chickpea collar rot by co-inoculation of antagonistic bacteria and compatible rhizobia.two hundred and seven bacteria were isolated from composts and macrofauna and screened for plant growth promoting and antagonistic traits. seven of the 207 isolates showed antagonistic activity against sclerotium rolfsii in plate culture. inhibition of s. rolfsii by the bacterial isolates ranged between 61 and 84%. two of the seven isolates were bacillus sp. and rest belonged to pseudomonas sp. two isolates, pseudomonas sp. cdb 35 and pseudomonas sp. bwb 21 was compatible with chickpea rhizobium ...201122282609
isolation of paenibacillus sp. and variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction.prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases and hemicellulases that could be useful in second-generation bioethanol production. in this work, cellulolytic bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. there was a predominance of gram positive isolates that belonged to the phyla proteobacteria and firmicutes. many primary isolates with cellulolytic activity were not p ...201223301200
characterisation of phosphate solubilising bacteria in sandy loam soil under chickpea cropping system.with the aim to explore the possible role of phosphate-solubilizing bacteria (psb) in phosphorus (p) cycling in agricultural soils, we isolated psb inhabiting naturally in the sandy loam soils under chickpea cropping of patiala (punjab state). a total of 31 bacterial isolates showing solubilizing activities were isolated on pikovskaya agar plates. the potent phosphate solubilizers were selected for further characterization. these isolates were shown to belong to the genera pseudomonas and serrat ...201123729877
characterisation of phosphate solubilising bacteria in sandy loam soil under chickpea cropping system.with the aim to explore the possible role of phosphate-solubilizing bacteria (psb) in phosphorus (p) cycling in agricultural soils, we isolated psb inhabiting naturally in the sandy loam soils under chickpea cropping of patiala (punjab state). a total of 31 bacterial isolates showing solubilizing activities were isolated on pikovskaya agar plates. the potent phosphate solubilizers were selected for further characterization. these isolates were shown to belong to the genera pseudomonas and serrat ...201123729877
promise for plant pest control: root-associated pseudomonads with insecticidal activities.insects are an important and probably the most challenging pest to control in agriculture, in particular when they feed on belowground parts of plants. the application of synthetic pesticides is problematic owing to side effects on the environment, concerns for public health and the rapid development of resistance. entomopathogenic bacteria, notably bacillus thuringiensis and photorhabdus/xenorhabdus species, are promising alternatives to chemical insecticides, for they are able to efficiently k ...201323914197
the 29(th) annual symposium of the protein society, barcelona, spain, july 22-25, 2015. 201526452528
human contamination in public genome assemblies.contamination in genome assembly can lead to wrong or confusing results when using such genome as reference in sequence comparison. although bacterial contamination is well known, the problem of human-originated contamination received little attention. in this study we surveyed 45,735 available genome assemblies for evidence of human contamination. we used lineage specificity to distinguish between contamination and conservation. we found that 154 genome assemblies contain fragments that with hi ...201627611326
gene duplications in prokaryotes can be associated with environmental adaptation.gene duplication is a normal evolutionary process. if there is no selective advantage in keeping the duplicated gene, it is usually reduced to a pseudogene and disappears from the genome. however, some paralogs are retained. these gene products are likely to be beneficial to the organism, e.g. in adaptation to new environmental conditions. the aim of our analysis is to investigate the properties of paralog-forming genes in prokaryotes, and to analyse the role of these retained paralogs by relati ...201020961426
comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights.persistence of microorganisms or reinfections are the main reasons for failure of root canal therapy. very few studies to date have included culture-independent methods to assess the microbiota, including non-cultivable microorganisms. the aim of this study was to combine culture methods with culture-independent cloning methods to analyze the microbial flora of root-filled teeth with periradicular lesions. twenty-one samples from previously root-filled teeth were collected from patients with per ...201223152922
newly identified pathogens associated with periodontitis: a systematic review.there is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. however, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. the aim of this systematic re ...201425074492
massive comparative genomic analysis reveals convergent evolution of specialized bacteria.genome size and gene content in bacteria are associated with their lifestyles. obligate intracellular bacteria (i.e., mutualists and parasites) have small genomes that derived from larger free-living bacterial ancestors; however, the different steps of bacterial specialization from free-living to intracellular lifestyle have not been studied comprehensively. the growing number of available sequenced genomes makes it possible to perform a statistical comparative analysis of 317 genomes from bacte ...200919361336
kinetics of dithionite-dependent reduction of cytochrome p450 3a4: heterogeneity of the enzyme caused by its oligomerization.to explore the basis of apparent conformational heterogeneity of cytochrome p450 3a4 (cyp3a4), the kinetics of dithionite-dependent reduction was studied in solution, in proteoliposomes, and in nanodiscs. in cyp3a4 oligomers in solution the kinetics obeys a three-exponential equation with similar amplitudes of each of the phases. addition of substrate (bromocriptine) displaces the phase distribution toward the slow phase at the expense of the fast one, while the middle phase remains unaffected. ...200516229479
functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter.targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ...201324062736
sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids.the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ...200919857494
sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids.the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ...200919857494
biotechnological tools for environmental sustainability: prospects and challenges for environments in nigeria-a standard review.the environment is a very important component necessary for the existence of both man and other biotic organisms. the degree of sustainability of the physical environment is an index of the survival and well-being of the entire components in it. additionally, it is not sufficient to try disposing toxic/deleterious substances with any known method. the best method of sustaining the environment is such that returns back all the components (wastes) in a recyclable way so that the waste becomes usef ...201222611499
electronic waste - an emerging threat to the environment of urban india.electronic waste or e-waste is one of the emerging problems in developed and developing countries worldwide. it comprises of a multitude of components with valuable materials, some containing toxic substances, that can have an adverse impact on human health and the environment. previous studies show that india has generated 0.4 million tons of e-waste in 2010 which may increase to 0.5 to 0.6 million tons by 2013-2014. coupled with lack of appropriate infrastructural facilities and procedures for ...201424444377
a bioinformatic survey of distribution, conservation, and probable functions of luxr solo regulators in bacteria.luxr solo transcriptional regulators contain both an autoinducer binding domain (abd; n-terminal) and a dna binding helix-turn-helix domain (hth; c-terminal), but are not associated with a cognate n-acyl homoserine lactone (ahl) synthase coding gene in the same genome. although a few luxr solos have been characterized, their distributions as well as their role in bacterial signal perception and other processes are poorly understood. in this study we have carried out a systematic survey of distri ...201525759807
functional promiscuity of homologues of the bacterial arsa atpases.the arsa atpase of e. coli plays an essential role in arsenic detoxification. published evidence implicates arsa in the energization of as(iii) efflux via the formation of an oxyanion-translocating complex with arsb. in addition, eukaryotic arsa homologues have several recognized functions unrelated to arsenic resistance. by aligning arsa homologues, constructing phylogenetic trees, examining arsa encoding operons, and estimating the probable coevolution of these homologues with putative transpo ...201020981284
the quest for a unified view of bacterial land colonization.exploring molecular mechanisms underlying bacterial water-to-land transition represents a critical start toward a better understanding of the functioning and stability of the terrestrial ecosystems. here, we perform comprehensive analyses based on a large variety of bacteria by integrating taxonomic, phylogenetic and metagenomic data, in the quest for a unified view that elucidates genomic, evolutionary and ecological dynamics of the marine progenitors in adapting to nonaquatic environments. we ...201424451209
mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa.bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ...201626712005
mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa.bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ...201626712005
phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils.phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. although abundant in soils, in both organic and inorganic forms, its availability is restricted as it occurs mostly in insoluble forms. the p content in average soil is about 0.05% (w/w) but only 0.1% of the total p is available to plant because of poor solubility and its fixation in soil (illmer and schinner, soil biol biochem 27:257-263, 1995). an adequate supply of p ...201325674415
new insights into 1-aminocyclopropane-1-carboxylate (acc) deaminase phylogeny, evolution and ecological significance.the main objective of this work is the study of the phylogeny, evolution and ecological importance of the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase, the activity of which represents one of the most important and studied mechanisms used by plant growth-promoting microorganisms. the acc deaminase gene and its regulatory elements presence in completely sequenced organisms was verified by multiple searches in diverse databases, and based on the data obtained a comprehensive analysis w ...201424905353
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