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in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila.the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ...19806448581
enumeration of potentially pathogenic bacteria from sewage sludges.to ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. a processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers of viable bacteria. conventional methods were successful in enumerating klebsiella, staphylococcus, gram-negative enteric bacteria, and commonly used indicator organisms. modificatio ...19806243900
cultural and biochemical characteristics of clinical isolates of unusual colistin-resistant pseudomonads.biochemical characteristics and antibiotic susceptibilities of 12 strains of colistin-resistant pseudomonads isolated from clinical specimens are reported. the isolates were short, oxidase-positive, nonfluorescing, gram-negative rods that failed to grow on salmonella-shigella or cetrimide agars, to decarboxylate amino acids, and to reduce nitrates. most strains peptonized litmus milk and grew at 42 degrees c. glucose, lactose, maltose, xylose, and fructose were slowly oxidized, whereas sucrose w ...19807229004
anaerobic transfer of antibiotic resistance from pseudomonas aeruginosa.pseudomonas aeruginosa, an opportunistic pathogen that often initiates infections from a reservoir in the intestinal tract, may donate or acquire antibiotic resistance in an anaerobic environment. only by including nitrate and nitrite in media could antibiotic-resistant and -sensitive strains of p. aeruginosa be cultured in a glove box isolator. these anaerobically grown cells remained sensitive to lytic phage isolated from sewage. after incubation with a phage lysate derived from p. aeruginosa ...19806773475
bacterial effect of hydrogen peroxide on urinary tract pathogens.bacterial contamination of urinary drainage bags is a frequent source of bladder bacteriuria in patients with indwelling catheters. previous work demonstrated that the addition of 30 ml of 3% h2o2 prevented bacterial contamination of urinary drainage bags for up to 8 h in patients with urinary infections (greater than 10(5) colony-forming units per ml). survival curves of a variety of organisms in filter-sterilized urine with various concentrations of h2o2 (0.6 to 0.01%) were constructed. organi ...19806781405
automated methods for identification of bacteria from clinical specimens.automated methods for measuring enzyme activities of bacterial suspensions in saline are described. the methods were applied to bacteria cultured from urine specimens, and specific enzyme profiles characteristic for escherichia coli, klebsiella sp, proteus sp, and pseudomonas sp were established. identification of 294 freshly isolated strains by automated and conventional methods were compared. results from automated identification based on eight enzyme tests and assay of protein content, all pe ...19806987274
pkj1, a naturally occurring conjugative plasmid coding for toluene degradation and resistance to streptomycin and sulfonamides.pseudomonas sp. ta8 isolated by m-toluate enrichment from an aqueous sample metabolized toluene and m- and p-xylenes via the meta cleavage pathway, and manifested specific resistance to streptomycin and sulfonamides. a variety of experiments revealed that the pkj1 plasmid of about 150 megadaltons carried by ta8 specified both the toluene and xylene degradative function (the tol function) and streptomycin/sulfonamide resistance. the deletion of a segment of pkj1 (about 22 megadaltons) resulted in ...19807009551
chemical structure and biodegradability of halogenated aromatic compounds. halogenated muconic acids as intermediates.substituted muconic acids were prepared from the corresponding catechols by pyrocatechase ii from pseudomonas sp. b13. the stabilities of substituted muconic acids were compared under different ph conditions. 3-substituted cis, cis-muconic acids cycloisomerized readily in slightly acidic solutions, whereas 2-chloro- and 2-fluoro-cis,cis-muconic acids were stable under these conditions and could be isolated as crystalline compounds. they were isomerized to the cis, trans-form in highly acidic sol ...19807305905
chemical structure and biodegradability of halogenated aromatic compounds. conversion of chlorinated muconic acids into maleoylacetic acid.1. an enzyme for the cycloisomerization of 2- and 3-chloro-cis,cis-muconic acid was isolated from 3-chlorobenzoate-grown cells of pseudomonas sp. b13. it was named muconate cycloisomerase ii, because it could it clearly be differentiated by its km and vmax. values from an ordinary muconate cycloisomerase, which functioned in benzoate catabolism and exhibited low activity with the chlorinated substrates. 2-chloro-cis,cis-muconic acid was converted into trans- and 3-chloro-cis,cis--muconic acid in ...19807305906
hybrid pathway for chlorobenzoate metabolism in pseudomonas sp. b13 derivatives.derivatives of pseudomonas sp. b13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the tol plasmid of pseudomonas putida mt-2 were studied. the utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. during growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deo ...19807380800
transport of methylamine by pseudomonas sp. ma.pseudomonas sp. ma grows on methylamines as a sole source of carbon, nitrogen, and energy. the transport of methylamine into the organism was investigated. it was found that this organism possesses an inducible transport system for methylamine having the following physical parameters: ph optimum, 7.2; temperature optimum, 30 to 35 degrees c; km, 1 to 30 mm; vmax, 90 to 120 nmol/min per mg (dry weight) of cells. methylamine uptake was curtailed by azide, cyanide, and carbonyl cyanide-m-chlorophen ...19807380809
microbial activity of trench leachates from shallow-land, low-level radioactive waste disposal sites.trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. among the several aerobic and anaerobic bacteria isolated, only bacillus sp., pseudomonas sp., citrobacter sp., and clostridium sp. were identified. mixed bacterial cultures isolated from the trench leachates were able to grow anaerobically in trench leachates, which indicates that th ...19807406490
enzymes involved in 3,5-diaminohexanoate degradation by brevibacterium sp.cell-free extracts of brevibacterium sp. l5 grown on dl-erythro-3,5-diaminohexanoate were found to contain a 3-keto-5-aminohexanoate cleavage enzyme that converts 3-keto-5-aminohexanoate and acetyl-coenzyme a (coka) to 3-aminobutyryl-coa and acetoacetate and a deaminase that coverts l-3-aminobutyryl-coa to crotonyl-coa. the cleavage enzyme has been purified extensively, and some of its properties have been determined for comparison with the 3-keto-6-acetamido-hexanoate cleavage enzyme of pseudom ...19807410315
deoxyribonucleic acid polymerase from the marine pseudomonas sp. bal-31.a deoxyribonucleic acid (dna)-dependent dna polymerase (dna nucleotidyltransferase) was purified 3,000-fold from the marine pseuodomonas sp. bal-31. the molecular weight of the native enzyme was estimated by glycerol gradient sedimentation to be 110,000. the enzyme migrated in sodium dodecyl sulfate-acrylamide gels as a single polypeptide with a molecular weight of 105,000. an absolute requirement for divalent cation was satisfied by mg2+ or mn2+ at concentrations of 1 mm. monovalent cations at ...19807372571
adaptation of alcaligenes eutrophus b9 and pseudomonas sp. b13 to 2-fluorobenzoate as growth substrate.alcaligenes eutrophus b9 and pseudomonas sp. b13 could be adapted to 2-fluorobenzoate as the sole source of carbon and energy. the ability of the a. eutrophus b9 to use this new substrate is clearly based on the defective dihydrodihydroxybenzoate dehydrogenase. nontoxic 6-fluoro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid is accumulated instead of 3-fluorocatechol. about 84% of the substrate is dioxygenated to catechol and utilized via the 3-oxoadipate pathway. during continuous adaptation of ...198016345497
symbiotic utilization of polyvinyl alcohol by mixed cultures.polyvinyl alcohol (pva)-utilizing cultures were obtained from various sources. they were mixed cultures even after cyclical transfer to liquid and plate media with pva as a sole source of carbon. component bacteria were isolated from the several mixed cultures, and it was shown that pva was utilized symbiotically by two bacterial members which could not utilize pva in each respective pure culture. from a mixed culture, strains vm15, vm15a (pseudomonas putida) and vm15c (pseudomonas sp.) were iso ...198116345693
root-associated n(2) fixation (acetylene reduction) by enterobacteriaceae and azospirillum strains in cold-climate spodosols.n(2) fixation by bacteria in associative symbiosis with washed roots of 13 poaceae and 8 other noncultivated plant species in finland was demonstrated by the acetylene reduction method. the roots most active in c(2)h(2) reduction were those of agrostis stolonifera, calamagrostis lanceolata, elytrigia repens, and phalaris arundinacea, which produced 538 to 1,510 nmol of c(2)h(4).g (dry weight). h when incubated at po(2) 0.04 with sucrose (ph 6.5), and 70 to 269 nmol of c(2)h(4). g (dry weight).h ...198116345687
recurrent septic arthritis due to pseudomonas sp.two episodes of pseudomonas septic arthritis occurred in an 84-year-old diabetic man separated by a 6 year interval. pseudomonas is an unusual cause of septic arthritis requiring vigorous antibiotic therapy. the recurrence of the infection may be related to the inadequate response to treatment in the first episode, however, the long interval remains unexplained.19817291109
characteristics of human isolates of unidentified fluorescence pseudomonads capable of growth at 42 degrees c.five strains of an unidentified fluorescent pseudomonas sp. which were capable of growth at 42 degrees c were isolated over a 3-year period and were examined and compared with chosen strains of pseudomonas aeruginosa, p. fluorescens, and p. putida. the strains were examined in a range of biochemical and carbon substrate alkalinization tests. the outstanding properties of the unidentified fluorescent pseudomonas sp. included monopolar arrangement of flagella, gelatin liquefaction, litmus milk pep ...19817309846
stimulation of bacterial arylsulfatase activity by arylamines: evidence for substrate activation.a number of arylamines (including tyramine and tryptamine) increased the in vitro activity of arylsulfatase from pseudomonas sp. strain c12b. amino acid analogs of these amines (e.g., tyrosine and tryptophan) failed to exert an effect. stimulation of activity by tyramine could not be accounted for in terms of sulfotransferase activity for this phenol, and no shift in the ph optimum for the enzyme occurred in the presence of tryptamine. increased vmax due to these amines was independent of enzyme ...19817240096
bacterial fouling of a hospital closed-loop cooling system by pseudomonas sp.during the summer of 1979 the air-conditioning system at a hospital in boston deteriorated, and this led to total failure of some chilling units. patient care and operating-room areas were affected. investigation of the problem ruled out mechanical and electrical causes, but revealed a strain of pseudomonas sp. biofouling heat transfer tubes of the closed chilled water system. the pseudomonads apparently were stimulated to grow by low concentrations of ethylene glycol antifreeze. the proximate s ...19817251827
bacterial growth in ground beef prepared from electrically stimulated and nonstimulated muscles.ground beef samples prepared from electrically stimulated and nonstimulated biceps femoris and infraspinatus muscles were inoculated with lactobacillus sp., pseudomonas sp., acinetobacter sp., or a mixture of lactobacillus spp., pseudomonas spp., acinetobacter spp., moraxella sp., microbacterium thermosphactum, and erwinia herbicola. there were no significant differences in growth of various bacteria in ground beef made from electrically stimulated and nonstimulated muscles.19817195194
arylsulfatase from pseudomonas sp. strain c12b: purification to homogeneity, immunological analysis, and physical properties.arylsulfatase was purified 219-fold from pseudomonas sp. strain c12b. the final preparation was homogeneous by electrophoretic and immunological analysis. the enzyme is a monomer of molecular weight about 51,000, with a stokes radius of 3.0 x 10(-7) cm, a frictional ratio of 1.2, and a sedimentation coefficient of 4.1s.19817204346
degradation of 4-chlorophenylacetic acid by a pseudomonas species.pseudomonas sp. strain cbs3 was able to utilize 4-chlorophenylacetic acid as the sole source of carbon and energy. when this strain was grown with 4-chlorophenylacetic acid, homoprotocatechuic acid was found to be an intermediate which was further metabolized by the meta-cleavage pathway. furthermore, three isomers of chlorohydroxyphenylacetic acid, two of them identified as 3-chloro-4-hydroxyphenylacetic acid and 4-chloro-3-hydroxyphenylacetic acid, were isolated from the culture medium. 4-hydr ...19817217006
the degradative pathway of the s-triazine melamine. the steps to ring cleavage.1. the degradative pathway of melamine (1,3,5-triazine-2,4,6-triamine) was examined in pseudomonas sp. strain a. 2. the bacterium grew with melamine, ammeline, ammelide, cyanuric acid or nh+4 as sole source of nitrogen, and each substrate was entirely metabolized. utilization of ammeline, ammelide, cyanuric acid or nh+4 was concomitant with growth. but with melamine as substrate, a transient intermediate was detected, which was identified as ammeline by three methods. 3. enzymes from strain a we ...19826762212
degradation of 3-phenylbutyric acid by pseudomonas sp.pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-pb) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. during early exponential growth, a catechol substance identified as 3-(2,3-dihydroxyphenyl)butyrate (2,3-dhpb) and its meta-cleavage product 2-hydroxy-7-methyl-6-oxononadioic-2,4-dienoic acid were produced. these products disappeared during late exponential ...19827118830
aerobic catabolism of bile acids.seventy-eight stable cultures obtained by enrichment on media containing ox bile or a single bile acid were able to utilize one or more bile acids, as well as components of ox bile, as primary carbon sources for growth. all isolates were obligate aerobes, and most (70) were typical (48) or atypical (22) pseudomonas strains, the remainder (8) being gram-positive actinomycetes. of six pseudomonas isolates selected for further study, five produced predominantly acidic catabolites after growth on gl ...19827149711
oxidation of naphthalene by a multicomponent enzyme system from pseudomonas sp. strain ncib 9816.the initial reactions in the oxidation of naphthalene by pseudomonas sp. strain ncib 9816 involves the enzymatic incorporation of one molecule of oxygen into the aromatic nucleus to form (+)-cis-(1r,2s)-dihydroxy-1,2-dihydronaphthalene. the enzyme catalyzing this reaction, naphthalene dioxygenase, was resolved into three protein components, designated a, b, and c, by deae-cellulose chromatography. incubation of naphthalene with components a, b, and c in the presence of nadh resulted in the forma ...19827037744
methylamine uptake in pseudomonas species strain ma: utilization of methylamine as the sole nitrogen source.the uptake of methylamine as the sole nitrogen, but not carbon, source by pseudomonas sp. strain ma was investigated. under these growth conditions, a high-affinity, low-capacity uptake system was present having a km of 16 microm and vmax of 2 nmol.min-.mg (dry weight) of cells that was competitively inhibited by ammonium chloride. the transport system was induced by growth on succinate with methylamine as the sole nitrogen source.19827054149
tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: enzyme regulation and dna structure.wr211 and wr216 are derivatives of halobenzoate-degrading pseudomonas sp. strain b13 into which the 117-kilobase tol degradative plasmid pww0 has been transferred from pseudomonas putida mt-2. wr211 has lost the ability to grow on the tol-specific substrate m-xylene but retains the ability to grow on its metabolite, m-toluate. an analysis of the induction of enzymes was consistent with wr211 carrying a nonfunctional regulatory gene, xy1r, wr216 is a spontaneous derivative of wr211 which grows on ...19827061391
excision and integration of degradative pathway genes from tol plasmid pww0.wr211 is a transconjugant resulting from transfer of the 117-kilobase (kb) tol degradative plasmid pww0 into pseudomonas sp. strain b13. the plasmid of this strain, pww01211, is 78 kb long, having suffered a deletion of 39 kb. we show that wr211 contains the 39 kb that is missing from its plasmid, together with at least an additional 17 kb of pww0 dna integrated in another part of the genome, probably the chromosome. the ability of wr211 to grow on the tol-specific substrate m-toluate is the res ...19827061392
tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: prevention of meta pathway.the hybrid pathway for chlorobenzoate metabolism was studied in wr211 and wr216, which were derived from pseudomonas sp. b13 by acquisition of tol plasmid pww0 from pseudomonas putida mt-2. chlorobenzoates are utilized readily by these strains when meta cleavage of chlorocatechols is suppressed. when wr211 utilizes 3-chlorobenzoate (3cb), the expression of catechol 2,3-dioxygenase (c23o) and the catabolic activities for chloroaromatics via the ortho pathway coexist as a consequence of inactivati ...19827061393
purification and properties of a new enzyme, dl-2-haloacid dehalogenase, from pseudomonas sp.a new enzyme, dl-2-haloacid dehalogenase, was isolated and purified to homogeneity from the cells of pseudomonas sp. strain 113. this enzyme catalyzed non-stereospecific dehalogenation of both of the optical isomers of 2-chloropropionate through an sn2 type of reaction; l- and d-lactates were formed from d- and l-2-chloropropionates, respectively. the enzyme acted on 2-halogenated aliphatic carboxylic acids whose carbon chain lengths were less than five. it also dehalogenated trichloroacetate to ...19827068529
in vitro activity of moxalactam and mecillinam, singly and in combination, against multi-drug-resistant enterobacteriaceae and pseudomonas species.the in vitro interaction of moxalactam and mecillinam against multi-drug-resistant gram-negative enteric bacilli was studied by checkerboard microdilution susceptibility tests and by killing curve kinetics. against enterobacteriaceae, the combination was unpredictable; the frequencies of synergy, indifference, and antagonism were 11, 76, and 13%, respectively. against pseudomonas sp., the two drugs were consistently indifferent. overall, the combination of moxalactam and mecillinam was no more a ...19826282206
broad-host-range incp-4 plasmid r1162: effects of deletions and insertions on plasmid maintenance and host range.r1162 is an 8.7-kilobase (kb) broad-host-range replicon encoding resistance to streptomycin and sulfa drugs. in vitro deletion of 1.8-kb dna between coordinates 3.0 and 5.3 kb did not affect plasmid maintenance, but a tn1 insertion at coordinate 6.3 kb led to a recessive defect in plasmid maintenance. the only cis-acting region necessary for plasmid replication appears to lie between the tn1 insertion at coordinate 6.3 kb and a second tn1 insertion at coordinate 6.5 kb. all r1162 sequences betwe ...19826288654
genetic homology between independently isolated chlorobenzoate-degradative plasmids.two chlorobenzoate-degradative plasmids were studied by the hybridization of the restriction endonuclease-generated fragments of one plasmid after transfer to a nitrocellulose filter with nick-translated radioactive dna of the other plasmid as a probe. two strains harboring the 3-chlorobenzoic acid-degradative plasmids were isolated in two different parts of the world at two different times. the plasmids are now found to be closely related to each other by hybridization studies. the chlorobenzoa ...19836294059
localization of polyamine enhancement of protein synthesis to subcellular components of escherichia coli and pseudomonas sp. strain kim.at 5 mm mg2+, spermidine stimulation of polyphenylalanine synthesis by cell-free extracts of escherichia coli was found to be about 30 times greater than that by extracts of pseudomonas sp. strain kim, a unique organism which lacks detectable levels of spermidine. by means of reconstitution experiments, the target of spermidine stimulation was localized to the protein fraction of the highspeed supernatant component (s-100) of e. coli and was absent from, or deficient in, the s-100 fraction of ps ...19836336736
ecological relationships between vibrio cholerae and planktonic crustacean copepods.strains of vibrio cholerae, both o1 and non-o1 serovars, were found to attach to the surfaces of live copepods maintained in natural water samples collected from the chesapeake bay and bangladesh environs. the specificity of attachment of v. cholerae to live copepods was confirmed by scanning electron microscopy, which revealed that the oral region and egg sac were the most heavily colonized areas of the copepods. in addition, survival of v. cholerae in water was extended in the presence of live ...19836337551
molecular cloning of the pseudomonas carboxypeptidase g2 gene and its expression in escherichia coli and pseudomonas putida.the gene coding for carboxypeptidase g2 was cloned from pseudomonas sp. strain rs-16 into escherichia coli w5445 by inserting sau3a-generated dna fragments into the bamhi site of pbr322. the plasmid isolated, pnm1, was restriction mapped, and the position of the gene on the 5.8-megadalton insert was pinpointed by subcloning. the expression of carboxypeptidase in e. coli was 100-fold lower than in the pseudomonas sp. strain. when the cloned gene was subcloned into the pseudomonas vector pkt230 an ...19836358192
properties and roles of bacterial symbionts of polyvinyl alcohol-utilizing mixed cultures.from several polyvinyl alcohol (pva)-utilizing mixed cultures, two component bacterial strains essential for pva utilization were isolated, and their properties and roles in pva utilization were studied. each pair of essential component strains consisted of a type i strain, which produced a pva-degrading enzyme and constituted the predominant population of the mixed culture in pva, and a type ii strain, which produced a certain growth stimulant for the former strain. all of the type i strains we ...19836639015
degradation of chlorophenols by a defined mixed microbial community.synthetic sewage containing phenol, acetone, and alkanols plus 4-chlorophenol or a mixture of isomeric chlorophenols is completely degraded by a defined mixed culture with pseudomonas sp. strain b13 as a chlorocatechol-dissimilating member of the community. total degradation of the organic carbon was indicated by release of stoichiometric amounts of chloride and low content of dissolved organic carbon in the cell-free effluents. during adaptation to high loads of chlorophenols the initial meta-c ...19836651293
the degradation of cholic acid by pseudomonas sp. n.c.i.b. 10590 under anaerobic conditions.the bacterial degradation of cholic acid under anaerobic conditions by pseudomonas sp. n.c.i.b. 10590 was studied. the major unsaturated neutral compound was identified as 12 beta-hydroxyandrosta-4,6-diene-3,17-dione, and the major unsaturated acidic metabolite was identified as 12 alpha-hydroxy-3-oxochola-4,6-dien-24-oic acid. eight minor unsaturated metabolites were isolated and evidence is given for the following structures: 12 alpha-hydroxyandrosta-4,6-diene-3,17-dione, 12 beta,17 beta-dihyd ...19836667260
transposable element that causes mutations in a plant pathogenic pseudomonas sp.a 1.3-kilobase-pair dna element, is51, causes a loss of virulence in the plant pathogen pseudomonas syringae pv. savastanoi. this sequence, is51, was first discovered in a plasmid-borne iaam locus, which together with iaah directs the synthesis of a virulence factor, indoleacetic acid. the spontaneous insertion of is51 in iaam resulted in the loss of indoleacetic acid production, attenuation of virulence, and the loss of both enzyme activities coded by iaam and iaah. using a cloned is51 element ...19836304006
uptake of methylamine and methanol by pseudomonas sp. strain am1.the uptake of methylamine and of methanol by the facultative methylotroph pseudomonas sp. strain am1 was investigated. it was found that this organism possesses two uptake systems for methylamine. one of these operates when methylamine is the sole source of carbon, nitrogen, and energy. it has a km of 1.33 x 10(-4) m and a vmax of 67 nmol/min per mg of cells (dry weight). the other system, found when methylamine is the sole nitrogen source only, has a km of 1.2 x 10(-5) m and a vmax of 8.9 nmol/ ...19836304007
studies on well water and possible health risks in katsina, nigeria.well water was sampled from all four major wards in katsina town. all 20 samples taken showed high coliform counts. sixty-five per cent contained greater than or equal to 2400 coliforms per 100 ml while the remainder had counts ranging from 79 to 920. faecal coliforms and non-cholera vibrios were detected in all samples. there was no significant relationship between the coliform counts and the distances of latrines to wells, water table to ground level, slope relationship between wells and latri ...19836833745
deoxycholic acid degradation by a pseudomonas sp. acidic intermediates with a-ring unsaturation.the microbial catabolism of deoxycholic acid by a pseudomonas sp. was studied, and six further acidic intermediates were isolated, as their methyl esters. evidence is presented that the compounds are methyl 12 alpha-hydroxy-3-oxochol-4-en-24-oate, methyl 12 alpha-hydroxy-3-oxo-23,24-dinorchol-4-en-22-oate, methyl 12 alpha-hydroxy-3-oxochola-1,4-dien-24-oate, methyl 12 alpha-hydroxy-3-oxo-23,24-dinorchola-1,4-dien-22-oate, methyl 12 alpha-hydroxy-3-oxochola-1,4,22e-trien-24-oate and methyl 12 alp ...19836870808
naphthalene dioxygenase: purification and properties of a terminal oxygenase component.naphthalene dioxygenase from pseudomonas sp. strain ncib 9816 is a multicomponent enzyme system that oxidized naphthalene to cis-(1r, 2s)-dihydroxy-1,2-dihydronaphthalene. the terminal oxygenase component b was purified to homogeneity by a three-step procedure that utilized ion-exchange and hydrophobic interaction chromatography. the purified enzyme oxidized naphthalene only in the presence of nadh, oxygen, and partially purified preparations of components a and c. an estimated mr of 158,000 was ...19836874638
nitrogenase activity (acetylene reduction) of root-associated, cold-climate azospirillum, enterobacter, klebsiella, and pseudomonas species during growth on various carbon sources and at various partial pressures of oxygen.a comprehensive view of the diazotrophic bacterial flora of plants requires that attention be paid to the appropriate carbon and oxygen requirements during isolation of the bacteria. twenty compounds (monosaccharides, disaccharides, polyols, and organic acids) were therefore examined as carbon and energy sources for nitrogenase activity in semisolid stab cultures at po(2) values of 0.21, 0.02, and </=0.002 with 12 strains of diazotrophic root-associated bacteria. with the facultatively anaerobic ...198316346205
iron-chelating compounds produced by soil pseudomonads: correlation with fungal growth inhibition.strains of pseudomonas putida, pseudomonas sp., and pseudomonas aeruginosa were examined for their ability to grow in the presence of the iron chelator, ethylenediamine-di-(o-hydroxyphenylacetic acid). in vitro fungal inhibition assays showed that the isolates varied in their ability to inhibit the growth of representative fungal plant pathogens. fungal inhibition in vitro was superior to that of previously reported pseudomonas sp. studies with fusarium oxysporum forma sp. lycopersici and a susc ...198316346334
isolation of a pseudomonas sp. which utilizes the phosphonate herbicide glyphosate.a strain of bacteria has been isolated which rapidly and efficiently utilizes the herbicide glyphosate (n-phosphonomethylglycine) as its sole phosphorus source in a synthetic medium. the strain (pg2982) was isolated by subculturing pseudomonas aeruginosa atcc 9027 in a synthetic broth medium containing glyphosate as the sole phosphorus source. strain pg2982 differs from the culture of p. aeruginosa in that it is nonflagellated, does not produce pyocyanin, and has an absolute requirement for thia ...198316346357
mixed continuous cultures of polyvinyl alcohol-utilizing symbionts pseudomonas putida vm15a and pseudomonas sp. strain vm15c.stable mixed continuous cultures of pseudomonas sp. strain vm15c and pseudomonas putida vm15a, the former of which produced a polyvinyl alcohol (pva)-degrading enzyme and the latter of which produced an essential growth factor for pva utilization by strain vm15c, were established with pva as the sole source of carbon and energy with chemostat cultivation. a high extent of pva degradation was achieved at dilution rates of less than 0.030/h. the predominant strain in the cultures was the primary m ...198416346642
molecular cloning of a malyl coenzyme a lyase gene from pseudomonas sp. strain am1, a facultative methylotroph.a genomic library containing hindiii partial digests of pseudomonas sp. strain am1 dna was constructed in the broad-host-range cosmid pvk100. pct57, a pseudomonas sp. strain am1 methanol mutant deficient in malyl coenzyme a lyase activity, was complemented to a methanol-positive phenotype by mobilization of the pvk100 library into pct57 recipients with the cole1/rk2 mobilizing plasmid prk2013. six different complemented isolates all contained a recombinant plasmid carrying the same 19.6-kilobase ...19846094488
purification and properties of the myo-inositol-binding protein from a pseudomonas sp.a myo-inositol-binding protein was isolated from a pseudomonas sp. soil isolate and was purified to homogeneity. its molecular weight is 30,000, and it has a single binding site. the amino acid analysis showed that the protein contains three tryptophan residues and no cysteine. tryptophan residues seem to be involved in the binding of the ligand, as shown by the modification of the fluorescence spectra and by the fact that oxidation of tryptophan residues with n-bromosuccinimide abolished the bi ...19846735978
enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by pseudomonas sp. strain b13.dna fragments containing the xyld and xyll genes of tol plasmid pww0 -161 of pseudomonas putida, which code for the catabolic enzymes toluate 1,2-dioxygenase and dihydrodihydroxybenzoic acid dehydrogenase, respectively, and the nahg gene of the nah plasmid nah7 , which codes for salicylate hydroxylase, were cloned in pbr322 vector plasmid. deletion and insertion mutagenesis were used to localize these genes with respect to crucial endonuclease cleavage sites. the pbr322-based plasmids were ligat ...19846327621
models for mineralization kinetics with the variables of substrate concentration and population density.the rates of mineralization of [14c]benzoate by an induced population of pseudomonas sp. were measured at initial substrate concentrations ranging from 10 ng/ml to 100 micrograms/ml. plots of the radioactivity remaining in the culture were fit by nonlinear regression to six kinetic models derived from the monod equation. these models incorporate only the variables of substrate concentration and cell density. plots of the mineralization kinetics in cultures containing low, intermediate, and high ...19846742843
in situ identification of bacterial species in marine microfouling films by using an immunofluorescence technique.an immunofluorescence technique was developed for the in situ identification of specific bacteria in marine microfouling films. microorganisms adherent to glass plates after 30 days of immersion in a synthetic seawater system were cultured and classified by biochemical tests, flagellar arrangement, and the api 20e system. all isolates were gram-negative aerobic or facultative motile rods, predominantly pseudomonas spp. rabbit antisera to the five dominant organisms including achromobacter spp., ...19846393875
secondary substrate utilization of methylene chloride by an isolated strain of pseudomonas sp.secondary substrate utilization of methylene chloride was analyzed by using pseudomonas sp. strain lp. both batch and continuously fed reactors demonstrated that this strain was capable of simultaneously consuming two substrates at different concentrations: the primary substrate at the higher concentration (milligrams per liter) and the secondary substrate at the lower concentration (micrograms per liter). the rate of methylene chloride utilization at trace concentrations was greater in the pres ...19846721491
2-chloro-4-amino-1,3,5-triazine-6(5h)-one: a new intermediate in the biodegradation of chlorinated s-triazines.pseudomonas sp. strain a grew with 2-chloro-1,3,5-triazine-4,6-diamine as the sole and growth-limiting source of nitrogen. the substrate was utilized quantitatively and concomitantly with growth and with excretion of a product which was identified as 2-chloro-4-amino-1,3,5-triazine-6(5h)-one. the reaction yielded 1 mol of organic product and 1 mol of nh4+ per mol of substrate.19846486789
oxidation and dehalogenation of 4-chlorophenylacetate by a two-component enzyme system from pseudomonas sp. strain cbs3.in cell-free extracts from pseudomonas sp. strain cbs3 the conversion of 4-chlorophenylacetate to 3,4-dihydroxyphenylacetate was demonstrated. by sephacryl s-200 chromatography two protein fractions, a and b, were obtained which both were essential for enzyme activity. fe2+ and nadh were cofactors of the reaction. nadph also activated the enzyme, but less effectively than nadh. fad had no influence on enzyme activity. 4-hydroxyphenylacetate, 4-chloro-3-hydroxyphenylacetate, and 3-chloro-4-hydrox ...19846501216
cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting pseudomonas strain.a gene bank of dna from plant growth-promoting pseudomonas sp. strain b10 was constructed using the broad host-range conjugative cosmid plafr1. the recombinant cosmids contained insert dna averaging 21.5 kilobase pairs in length. nonfluorescent mutants of pseudomonas sp. strain b10 were obtained by mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine, ethyl methanesulfonate, or uv light and were defective in the biosynthesis of its yellow-green, fluorescent siderophore (microbial iron transport ...19846690426
nadp-dependent glutamate dehydrogenase from a facultative methylotroph, pseudomonas sp. strain am1.the nadp-dependent glutamate dehydrogenase (ec 1.4.1.4.) elaborated by the methylotrophic bacterium pseudomonas sp. strain am1 when growing on succinate and ammonium chloride was studied. the enzyme, which has a ph optimum of 9.0, was purified 140-fold and shown to have km values of 20.2 mm, 0.76 mm, 0.033 mm, and 31.6 mm for ammonia, alpha-ketoglutarate, nadph, and glutamate, respectively. the native molecular weight was determined by polyacrylamide gel electrophoresis to be 190,000, and electr ...19846693348
transport of mevalonate by pseudomonas sp. strain m.pseudomonas sp. m, isolated from soil by elective culture on r,s-mevalonate as the sole source of carbon, possessed an inducible transport system for mevalonate. this high-affinity system had a ph optimum of 7.0, a temperature optimum of 30 degrees c, a km for r,s-mevalonate of 88 microm, and a v max of 26 nmol of mevalonate transported per min/mg of cells (dry weight). transport was energy dependent since azide, cyanide, or m-chlorophenylhydrazone caused complete cessation of transport activity ...19846434521
multiple forms of gamma-butyrobetaine hydroxylase (ec 1.14.11.1).gamma-butyrobetaine hydroxylase [4-trimethylaminobutyrate, 2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating), ec 1.14.11.1] from human kidney was resolved into three forms by chromatofocusing. after further chromatography on an anion-exchanger, each form appeared as a single band on electrophoresis in polyacrylamide gel containing sodium dodecyl sulphate. the isoelectric points of isoenzymes 1, 2 and 3 were 5.6, 5.7 and 5.8 respectively, as estimated by isoelectric focusing. their specific ...19846497835
effect of restricted aeration on catabolism of cholic acid by two pseudomonas species.examination of some previously isolated bile acid-utilizing pseudomonas strains showed that pseudomonas sp. atcc 31752, together with other fluorescent strains, can be assigned to pseudomonas putida biotype b, whereas pseudomonas sp. atcc 31753, like most other nonfluorescent strains, is an unrecognized phenotype. a study was made of the growth of these two species at 25 degrees c and ph 7.0 in a fermentor with 2.5 g of sodium cholate liter-1 as sole carbon source, and the catabolism of the chol ...19846476826
effects of dissolved organic carbon and second substrates on the biodegradation of organic compounds at low concentrations.pseudomonas acidovorans and pseudomonas sp. strain anl but not salmonella typhimurium grew in an inorganic salts solution. the growth of p. acidovorans in this solution was not enhanced by the addition of 2.0 micrograms of phenol per liter, but the phenol was mineralized. mineralization of 2.0 micrograms of phenol per liter by p. acidovorans was delayed 16 h by 70 micrograms of acetate per liter, and the delay was lengthened by increasing acetate concentrations, whereas phenol and acetate were u ...19853890738
effects of antimicrobial agents fed to chickens on some gram-negative enteric bacilli.total and antimicrobial agent-resistant aerobic and facultative anaerobic gram-negative enteric bacilli in fecal samples of broiler chickens fed growth-promotional levels of antimicrobial agents were determined quantitatively. two 8-week studies were conducted utilizing groups of chickens fed antimicrobial-supplemented rations; the second study involved feed "pasteurization" as a means of minimizing colonization from the feed. dilution/spread-plating/replica-plating techniques on selective media ...19853852666
ring cleavage and degradative pathway of cyanuric acid in bacteria.the degradative pathway of cyanuric acid [1,3,5-triazine-2,4,6(1h,3h,5h)-trione] was examined in pseudomonas sp. strain d. the bacterium grew with cyanuric acid, biuret, urea or nh4+ as sole source of nitrogen, and each substrate was entirely metabolized concomitantly with growth. enzymes from strain d were separated by chromatography on deae-cellulose and three reactions were examined. cyanuric acid (1 mol) was converted stoichiometrically into 1.0 mol of co2 and 1.1 mol of biuret, which was co ...19853904735
nonlinear estimation of the parameters of monod kinetics that best describe mineralization of several substrate concentrations by dissimilar bacterial densities.the kinetics of mineralization of a wide range of concentrations of benzoate, glucose, and benzylamine by pseudomonas sp., salmonella typhimurium, and microorganisms in acclimated sewage was studied. the treatment of initial substrate concentration and population density as independent variables in nonlinear regression analysis permitted the estimation of a single value for each of the parameters of monod kinetics that best described the mineralization of substrate at each concentration by the p ...19853909965
purification and properties of s-adenosylmethionine: aldoxime o-methyltransferase from pseudomonas sp. n.c.i.b. 11652.an enzyme catalysing the o-methylation of isobutyraldoxime by s-adenosyl-l-methionine was isolated from pseudomonas sp. n.c.i.b. 11652. the enzyme was purified 220-fold by deae-cellulose chromatography, (nh4)2so4 fractionation, gel filtration on sephadex g-100 and chromatography on calcium phosphate gel. homogeneity of the enzyme preparation was confirmed by isoelectric focusing on polyacrylamide gel and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. the enzyme showed a narrow ph op ...19853977861
cloning of genes involved in myo-inositol transport in a pseudomonas sp.a soil isolate of a pseudomonas sp. can utilize myo-inositol (mi) as the sole carbon source. in this strain, mi is transported through the membrane by a high-affinity transport system in which a periplasmic binding protein is involved. mutants impaired in the transport system were obtained by mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine and subsequently identified by their slow growth rate at low mi concentrations. strains with a low linear initial rate of mi uptake were analyzed. using ...19853980439
autoscan-4 system for identification of gram-negative bacilli.a production model of the autoscan-4 system (american microscan, inc., mahwah, n.j.) was tested with not more than 11 strains each of 73 groups or species of gram-negative bacilli from various centers for disease control culture collections. the strains included typical and atypical strains of enteric fermenters, nonenteric fermenters, and nonfermenters. the autoscan-4 system identified 95.3% of all 405 cultures accurately: 95.4% of 307 members of the family enterobacteriaceae, 96.6% of 29 nonen ...19853905849
activities of pefloxacin and ciprofloxacin in experimentally induced pseudomonas pneumonia in neutropenic guinea pigs.pefloxacin and ciprofloxacin are two new quinoline carboxylic acid derivatives that have activity in vitro against a wide range of gram-negative bacteria, including pseudomonas aeruginosa. using a well-standardized model of pseudomonas pneumonia in neutropenic guinea pigs, we tested the efficacy in vivo of these new agents. both were highly effective in increasing survival and decreasing bacterial counts in the lungs of surviving animals. pefloxacin and ciprofloxacin were significantly better (p ...19853159336
localization of polyvinyl alcohol oxidase produced by a bacterial symbiont, pseudomonas sp. strain vm15c.an axenic culture of a polyvinyl alcohol (pva)-degrading symbiont, pseudomonas sp. strain vm15c, was established on pva with a crude preparation of the growth factor (factor a) produced by the symbiotic partner pseudomonas putida vm15a. an increase of factor a in the culture medium enhanced the cell-associated pva oxidase activity as well as the growth rate, but decreased production of extracellular pva oxidase. pva oxidase in cells grown on pva was present in the periplasmic space at a higher r ...198516346711
enhancement of pyrroloquinoline quinone production and polyvinyl alcohol degradation in mixed continuous cultures of pseudomonas putida vm15a and pseudomonas sp. strain vm15c with mixed carbon sources.in a mixed continuous culture of pseudomonas putida vm15a and pseudomonas sp. strain vm15c with polyvinyl alcohol (pva) as the sole source of carbon, growth of the pva-degrading bacterium vm15c and, hence, pva degradation were limited by the growth factor, pyrroloquinoline quinone, produced by vm15a. feeding of a carbon source for vm15a, ethanol, with pva enhanced pyrroloquinoline quinone production and caused increases in the vm15c population and pva degradation in a mixed continuous culture. t ...198516346804
effects of chemical speciation on the mineralization of organic compounds by microorganisms.the mineralization of 1.0 to 100 ng each of four complexing compounds-oxalate, citrate, nitrilotriacetate (nta), and edta-per ml was tested in media prepared in accordance with equilibrium calculations by a computer program so that the h, ca, mg, fe, or al complex (chemical species) was predominant. sewage microorganisms mineralized calcium citrate more rapidly than iron, aluminum, or hydrogen citrate, and magnesium citrate was degraded slowest. aluminum, hydrogen, and iron oxalates were mineral ...198516346854
isolation of endoglucanase genes from pseudomonas fluorescens subsp. cellulosa and a pseudomonas sp.endoglucanase genes from pseudomonas fluorescens subsp. cellulosa and pseudomonas sp. were cloned and characterized. dna hybridization studies showed that these genes are homologous and that each species has one copy of the gene per genome. the dna fragment from pseudomonas sp. codes for, at most, a 23-kilodalton endoglucanase.198616347092
inhibitor studies of dissimilative fe(iii) reduction by pseudomonas sp. strain 200 ("pseudomonas ferrireductans")aerobic respiration and dissimilative iron reduction were studied in pure, batch cultures of pseudomonas sp. strain 200 ("pseudomonas ferrireductans"). specific respiratory inhibitors were used to identify elements of electron transport chains involved in the reduction of molecular oxygen and fe(iii). when cells were grown at a high oxygen concentration, dissimilative iron reduction occurred via an abbreviated electron transport chain. the induction of alternative respiratory pathways resulted f ...19862428308
glyphosate catabolism by pseudomonas sp. strain pg2982.the pathway for the degradation of glyphosate (n-phosphonomethylglycine) by pseudomonas sp. pg2982 has been determined by using metabolic radiolabeling experiments. radiorespirometry experiments utilizing [3-14c]glyphosate revealed that approximately 50 to 59% of the c-3 carbon was oxidized to co2. fractionation of stationary-phase cells labeled with [3-14c]glyphosate revealed that from 45 to 47% of the assimilated label is distributed to proteins and that the amino acids methionine and serine a ...19862430939
purification and characterization of quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus l.m.d. 79.41.quinoprotein glucose dehydrogenase (ec 1.1.99.17) from acinetobacter calcoaceticus l.m.d. 79.41 was purified to homogeneity. it is a basic protein with an isoelectric point of 9.5 and an mr of 94,000. denaturation yields two molecules of pqq/molecule and a protein with an mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. the oxidized enzyme form has an absorption maximum at 350 nm, and the reduc ...19863800975
isolation and complementation analysis of 10 methanol oxidation mutant classes and identification of the methanol dehydrogenase structural gene of methylobacterium sp. strain am1.a method has been developed for the direct selection of methanol oxidation mutants of the facultative methylotroph methylobacterium sp. strain am1 (formerly pseudomonas sp. strain am1). using this direct selection technique, we have isolated mutants of methylobacterium sp. strain am1 that are no longer capable of growth on methanol but retain the ability to grow on methylamine. these methanol oxidation (mox) mutants were complemented with a genomic clone bank of this organism constructed in the ...19863009411
existence of a novel enzyme, pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, pseudomonas sp. strain vm15c.a novel enzyme, pyrroloquinoline quinone (pqq)-dependent polyvinyl alcohol (pva) dehydrogenase, was found in and partially purified from the membrane fraction of a pva-degrading symbiont, pseudomonas sp. strain vm15c. the enzyme required pqq for pva dehydrogenation with phenazine methosulfate, phenazine ethosulfate, and 2,6-dichlorophenolindophenol as electron acceptors and did not show pva oxidase activity leading to h2o2 formation. the enzyme was active toward low-molecular-weight secondary al ...19863513704
identification of legionella pneumophila with commercially available immunofluorescence test. 19863522642
microbial transformation of quinoline by a pseudomonas sp.a pseudomonas sp. isolated from sewage by enrichment culture on quinoline metabolized this substrate by a novel pathway involving 8-hydroxycoumarin. during early growth of the organism on quinoline, 2-hydroxyquinoline accumulated as the intermediate; 8-hydroxycoumarin accumulated as the major metabolite on further incubation. 2,8-dihydroxyquinoline and 2,3-dihydroxyphenylpropionic acid were identified as the other intermediates. inhibition of quinoline metabolism by 1 mm sodium arsenite led to t ...19863089153
toxicity of paraquat to microorganisms.the biochemical response of the microorganisms lipomyces starkeyi (lod & rij), escherichia coli k-12 w3110, bacillus subtilis 168 (marburg) and pseudomonas sp. strain tto1 to the presence of growth-inhibitory concentrations of paraquat was studied. paraquat was added to each culture at a concentration previously determined to reduce the culture growth rate by up to 50%. the changes in activity of a number of enzymes previously shown to be associated with the defense of the mammalian system again ...19863098166
cloning of pseudomonas sp. strain cbs3 genes specifying dehalogenation of 4-chlorobenzoate.the degradation of 4-chlorobenzoate (4-cba) by pseudomonas sp. strain cbs3 is thought to proceed first by the dehalogenation of 4-cba to 4-hydroxybenzoate (4-hba), which is then metabolized following the protocatechuate branch of the beta-ketoadipate pathway. the cloning of the 4-cba dehalogenation system was carried out by constructing a gene bank of pseudomonas sp. strain cbs3 in pseudomonas putida. hybrid plasmid ppsa843 contains a 9.5-kilobase-pair fragment derived from the chromosome of pse ...19863759912
the potential of carboxypeptidase g2-antibody conjugates as anti-tumour agents. i. preparation of antihuman chorionic gonadotrophin-carboxypeptidase g2 and cytotoxicity of the conjugate against jar choriocarcinoma cells in vitro.carboxypeptidase g2, a zinc metalloenzyme isolated from pseudomonas sp. strain rs-16, which catalyses the hydrolytic cleavage of reduced and non-reduced folates to pteroates and l-glutamate, has been linked to a monoclonal antibody (w14a) raised to human chorionic gonadotrophin. the coupling efficiency and retention of antibody and enzymatic activities are compared for three separate methods of preparing 1:1 conjugates. preliminary in vitro studies on the cytotoxicity of the free enzyme and the ...19863964540
discovery of a cutinase-producing pseudomonas sp. cohabiting with an apparently nitrogen-fixing corynebacterium sp. in the phyllosphere.a phyllospheric bacterial culture, previously reported to partially replace nitrogen fertilizer (b. r. patti and a. k. chandra, plant soil 61:419-427, 1981) was found to contain a fluorescent pseudomonas which was identified as pseudomonas putida and a corynebacterium sp. the p. putida isolate was found to produce an extracellular cutinase when grown in a medium containing cutin, the polyester structural component of plant cuticle. the corynebacterium sp. grew on nitrogen-free medium but could n ...19873793714
kinetics of p-nitrophenol mineralization by a pseudomonas sp.: effects of second substrates.the kinetics of simultaneous mineralization of p-nitrophenol (pnp) and glucose by pseudomonas sp. were evaluated by nonlinear regression analysis. pseudomonas sp. did not mineralize pnp at a concentration of 10 ng/ml but metabolized it at concentrations of 50 ng/ml or higher. the ks value for pnp mineralization by pseudomonas sp. was 1.1 micrograms/ml, whereas the ks values for phenol and glucose mineralization were 0.10 and 0.25 micrograms/ml, respectively. the addition of glucose to the media ...19873426223
an nad+-dependent alanine dehydrogenase from a methylotrophic bacterium.a study was made of the nad+-dependent alanine dehydrogenase (ec 1.4.1.1) elaborated by the methylotrophic bacterium pseudomonas sp. strain ma when growing on succinate and nh4cl. this enzyme was purified 400-fold and was found to be highly specific for nh3 and nad+; however, hydroxypyruvate and bromopyruvate, but not alpha-oxoglutarate or glyoxylate, could replace pyruvate to a limited extent. the mr of the native enzyme was shown to be 217,000, and electrophoresis in sds/polyacrylamide gels re ...19873446176
degradation of 1,4-dichlorobenzene by a pseudomonas sp.a pseudomonas species able to degrade p-dichlorobenzene as the sole source of carbon and energy was isolated by selective enrichment from activated sludge. the organism also grew well on chlorobenzene and benzene. washed cells released chloride in stoichiometric amounts from o-, m-, and p-dichlorobenzene, 2,5-dichlorophenol, 4-chlorophenol, 3-chlorocatechol, 4-chlorocatechol, and 3,6-dichlorocatechol. initial steps in the pathway for p-dichlorobenzene degradation were determined by isolation of ...19873606087
microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids.sulfur-limited batch enrichment cultures containing one of nine multisubstituted naphthalenesulfonates and an inoculum from sewage yielded several taxa of bacteria which could quantitatively utilize 19 sulfonated aromatic compounds as the sole sulfur source for growth. growth yields were about 4 kg of protein per mol of sulfur. specific degradation rates were about 4 to 14 mu kat/kg of protein. a pseudomonas sp., an arthrobacter sp., and an unidentified bacterium were examined. each desulfonated ...19873662502
molecular cloning and expression of the 3-chlorobenzoate-degrading genes from pseudomonas sp. strain b13.the genes specifying the utilization of 3-chlorobenzoate by pseudomonas sp. strain b13 wr1 have been cloned by using a broad-host-range cosmid cloning system. analysis of the catabolic products of the enzymatic reactions encoded by two hybrid cosmids, pmw65 and pmw90, by thin-layer and high-performance liquid chromatography demonstrated that both encoded the genes for the complete catabolism of 3-chlorobenzoate. physical analysis of one of the cosmid derivatives, pmw65, by restriction endonuclea ...19873025183
role of dissolution rate and solubility in biodegradation of aromatic compounds.strains of moraxella sp., pseudomonas sp., and flavobacterium sp. able to grow on biphenyl were isolated from sewage. the bacteria produced 2.3 to 4.5 g of protein per mol of biphenyl carbon, and similar protein yields were obtained when the isolates were grown on succinate. mineralization of biphenyl was exponential during the phase of exponential growth of moraxella sp. and pseudomonas sp. in biphenyl-supplemented media, flavobacterium sp. had one exponential phase of growth apparently at the ...19873566268
pseudomonas sp. group ve-2 bacterial peritonitis in a patient on continuous ambulatory peritoneal dialysis.pseudomonas sp. group ve-2 peritonitis occurred in a patient on continuous ambulatory peritoneal dialysis who had recently completed intraperitoneal cephalosporin therapy for culture-negative peritonitis. this is the second reported case of peritonitis in this population of patients due to this unusual organism, which is usually resistant to most cephalosporin antibiotics.19873571484
nucleotide sequences of the genes for two distinct cephalosporin acylases from a pseudomonas strain.two genes, acyi and acyii, for distinct cephalosporin acylases from pseudomonas sp. strain se83 (a. matsuda, k. matsuyama, k. yamamoto, s. ichikawa, and k.i. komatsu, j. bacteriol. 169:5815-5820, 1987) were sequenced. each sequence contained a single open reading frame for two nonidentical subunits, predicting a common precursor. some homologies at the amino acid level were found between the acyii-encoded enzyme, but not the acyi-encoded one, and other related acylases.19873680178
solid-state 13c nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil pseudomonas sp.we investigated concentration-dependent primary and secondary substrate relationships in the simultaneous metabolism of the ubiquitous pollutant phenol and the naturally occurring substrate acetate by a pseudomonas sp. soil isolate capable of utilizing either substance as a sole source of carbon and energy. in addition to conventional analytical techniques, solid-state 13c nuclear magnetic resonance spectroscopy was used to follow the cellular distribution of [1-13c]acetate in the presence of un ...19873827242
dienelactone hydrolase from pseudomonas sp. strain b13.dienelactone hydrolase (ec 3.1.1.45) catalyzes the conversion of cis- or trans-4-carboxymethylenebut-2-en-4-olide (dienelactone) to maleylacetate. an approximately 24-fold purification from extracts of 3-chlorobenzoate-grown pseudomonas sp. strain b13 yielded a homogeneous preparation of the enzyme. the purified enzyme crystallized readily and proved to be a monomer with a molecular weight of about 30,000. each dienelactone hydrolase molecule contains two cysteinyl side chains. one of these was ...19873804973
nucleotide sequence and expression of clcd, a plasmid-borne dienelactone hydrolase gene from pseudomonas sp. strain b13.the clcd structural gene encodes dienelactone hydrolase (ec 3.1.1.45), an enzyme that catalyzes the conversion of dienelactones to maleylacetate. the gene is part of the clc gene cluster involved in the utilization of chlorocatechol and is carried on a 4.3-kilobase-pair bglii fragment subcloned from the pseudomonas degradative plasmid pac27. a 1.9-kilobase-pair psti-ecori segment subcloned from the bglii fragment was shown to carry the clcd gene, which was expressed inducibly under the tac promo ...19873804974
analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfda of alcaligenes eutrophus jmp134.plasmid pjp4 of alcaligenes eutrophus jmp134 contains all genes for the degradation of 2,4-dichlorophenoxyacetic acid (2,4-d). five of these genes, tfdb, tfdc, tfdd, tfde, and tfdf, have recently been localized and cloned (r. h. don, a. j. weightman, h.-j. knackmuss, and k. n. timmis, j. bacteriol. 161:85-90, 1985). gene tfda, which codes for the 2,4-d monooxygenase, has now been found by mutagenesis with transposon tn5. a 3-kilobase fragment of pjp4 cloned in a broad-host-range vector could com ...19873036764
plasmid pcbi carries genes for anaerobic benzoate catabolism in alcaligenes xylosoxidans subsp. denitrificans pn-1.pseudomonas sp. strain pn-1 is reclassified as alcaligenes xylosoxidans subsp. denitrificans pn-1. strain pn-1 is a gram-negative, rod-shaped organism, is motile by means of lateral flagella, is oxidase positive, and does not ferment sugars. plasmid pcbi, carrying genes for the anaerobic degradation of benzoate in strain pn-1, is 17.4 kilobase pairs in length and is transmissible to a number of denitrifying pseudomonas aeruginosa and pseudomonas stutzeri strains. a restriction endonuclease map w ...19872822651
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