Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. | rhizosphere, the interface between soil and plant roots, is a chemically complex environment which supports the development and growth of diverse microbial communities. the composition of the rhizosphere microbiome is dynamic and controlled by multiple biotic and abiotic factors that include environmental parameters, physiochemical properties of the soil, biological activities of the plants and chemical signals from the plants and bacteria which inhabit the soil adherent to root-system. recent a ... | 2015 | 25926899 |
| the role of ala198 in the stability and coenzyme specificity of bacterial formate dehydrogenases. | it has been shown by an x-ray structural analysis that the amino acid residues ala198, which are located in the coenzyme-binding domain of nad(+)-dependent formate dehydrogenases (ec 1.2.1.2., fdh) from bacteria pseudomonas sp.101 and moraxella sp. c-1 (psefdh and morfdh, respectively), have non-optimal values of the angles ψ and φ. these residues were replaced with gly by site-directed mutagenesis. the mutants psefdh a198g and morfdh a198g were expressed in e.coli cells and obtained in active a ... | 2015 | 25927002 |
| transcriptional modulation of intestinal innate defense/inflammation genes by preterm infant microbiota in a humanized gnotobiotic mouse model. | it is known that postnatal functional maturation of the small intestine is facilitated by microbial colonization of the gut. preterm infants exhibit defects in gut maturation, weak innate immunity against intestinal infection and increased susceptibility to inflammatory disorders, all of which may be related to the inappropriate microbial colonization of their immature intestines. the earliest microbes to colonize the preterm infant gut encounter a naïve, immature intestine. thus this earliest m ... | 2015 | 25928420 |
| interactions of non-natural halogenated substrates with d-specific dehalogenase (dehd) mutants using in silico studies. | the d-2-haloacid dehalogenase of d-specific dehalogenase (dehd) from rhizobium sp. rc1 catalyses the hydrolytic dehalogenation of d-haloalkanoic acids, inverting the substrate-product configuration and thereby forming the corresponding l-hydroxyalkanoic acids. our investigations were focused on dehd mutants: r134a and y135a. we examined the possible interactions between these mutants with haloalkanoic acids and characterized the key catalytic residues in the wild-type dehalogenase, to design deh ... | 2014 | 26019583 |
| the potential impact of coinfection on antimicrobial chemotherapy and drug resistance. | across a range of pathogens, resistance to chemotherapy is a growing problem in both public health and animal health. despite the ubiquity of coinfection, and its potential effects on within-host biology, the role played by coinfecting pathogens on the evolution of resistance and efficacy of antimicrobial chemotherapy is rarely considered. in this review, we provide an overview of the mechanisms of interaction of coinfecting pathogens, ranging from immune modulation and resource modulation, to d ... | 2015 | 26028590 |
| lack of negative effects on syrian hamsters and mongolian gerbils housed in the same secondary enclosure. | in cases where different species might be housed in the same room or secondary enclosure, the guide for the care and use of laboratory animals recommends that the animals should be behaviorally compatible and have the same health status. syrian hamsters and mongolian gerbils, both desert-dwelling rodents, appear to be reasonable candidates for such a combination. this study was undertaken to evaluate whether housing hamsters and gerbils in the same secondary enclosure is an acceptable practice. ... | 2015 | 26045450 |
| efflux pump-deficient mutants as a platform to search for microbes that produce antibiotics. | pseudomonas putida dot-t1e-18 is a strain deficient in the major antibiotic efflux pump (ttgabc) that exhibits an overall increased susceptibility to a wide range of drugs when compared with the wild-type strain. we used this strain as a platform to search for microbes able to produce antibiotics that inhibit growth. a collection of 2400 isolates from soil, sediments and water was generated and a drop assay developed to identify, via growth inhibition halos, strains that prevent the growth of do ... | 2015 | 26059350 |
| seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient. | this research investigated spatial-temporal variation in benthic bacterial community structure, rates of denitrification and dissimilatory nitrate reduction to ammonium (dnra) processes and abundances of corresponding genes and transcripts at three sites-the estuary-head, mid-estuary and the estuary mouth (em) along the nitrate gradient of the colne estuary over an annual cycle. denitrification rates declined down the estuary, while dnra rates were higher at the estuary head and middle than the ... | 2015 | 26082763 |
| microbial population dynamics in response to pectobacterium atrosepticum infection in potato tubers. | endophytes are microbes and fungi that live inside plant tissues without damaging the host. herein we examine the dynamic changes in the endophytic bacterial community in potato (solanum tuberosum) tuber in response to pathogenic infection by pectobacterium atrosepticum, which causes soft rot in numerous economically important crops. we quantified community changes using both cultivation and next-generation sequencing of the 16s rrna gene and found that, despite observing significant variability ... | 2015 | 26118792 |
| abstracts, 9th german allergy congress, wiesbaden, 2-4 october 2014. | 2014 | 26120532 | |
| a facile method for controlling the reaction equilibrium of sphingolipid ceramide n-deacylase for lyso-glycosphingolipid production. | lyso-glycosphingolipids (lyso-gsls), the n-deacylated forms of glycosphingolipids (gsls), are important synthetic intermediates for the preparation of gsl analogs. although lyso-gsls can be produced by hydrolyzing natural gsls using sphingolipid ceramide n-deacylase (scdase), the yield for this reaction is usually low because scdase also catalyzes the reverse reaction, ultimately establishing an equilibrium between hydrolysis and synthesis. in the present study, we developed an efficient method ... | 2015 | 26130766 |
| analysis of fungal ball rhinosinusitis by culturing fungal clumps under endoscopic surgery. | this study was designed to investigate the clinical microbiology of fungal ball (fb) rhinosinusitis by culturing fungal clumps collected under endoscopic surgery. | 2015 | 26131186 |
| identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana fusarium wilt. | banana fusarium wilt (also known as panama disease) is one of the most disastrous plant diseases. effective control methods are still under exploring. the endophytic bacterial strain itbb b5-1 was isolated from the rubber tree, and identified as serratia marcescens by morphological, biochemical, and phylogenetic analyses. this strain exhibited a high potential for biological control against the banana fusarium disease. visual agar plate assay showed that itbb b5-1 restricted the mycelial growth ... | 2015 | 26133557 |
| classes of organic molecules targeted by a methanogenic microbial consortium grown on sedimentary rocks of various maturities. | organic-rich shales are populated by methanogenic consortia that are able to degrade the fossilized organic matter into methane gas. to identify the organic fraction effectively degraded, we have sequentially depleted two types of organic-rich sedimentary rocks, shale, and coal, at two different maturities, by successive solvent extractions to remove the most soluble fractions (maltenes and asphaltenes) and isolate kerogen. we show the ability of the consortia to produce methane from all rock sa ... | 2015 | 26136731 |
| encapsulating peritoneal sclerosis as a late complication of peritoneal dialysis. | encapsulating peritoneal sclerosis (eps) is a rare cause of intestinal obstruction which is characterized by fibrotic encapsulation of the bowel. although its pathogenesis is still not clear, many etiological factors have been stated. | 2015 | 26150908 |
| unearthing the genomes of plant-beneficial pseudomonas model strains wcs358, wcs374 and wcs417. | plant growth-promoting rhizobacteria (pgpr) can protect plants against pathogenic microbes through a diversity of mechanisms including competition for nutrients, production of antibiotics, and stimulation of the host immune system, a phenomenon called induced systemic resistance (isr). in the past 30 years, the pseudomonas spp. pgpr strains wcs358, wcs374 and wcs417 of the willie commelin scholten (wcs) collection have been studied in detail in pioneering papers on the molecular basis of pgpr-me ... | 2015 | 26198432 |
| bioaugmentation of mesorhizobium cicer, pseudomonas spp. and piriformospora indica for sustainable chickpea production. | chickpea establishes symbiotic association with mesorhizobium to fulfill its nitrogen (n) requirement. integrating chickpea rhizosphere with potential native mesorhizobia and other plant growth promoting microorganisms can contribute multiple benefits to plants. the present investigation was undertaken to study interactions among piriformospora indica (pi) with potential plant growth promoting rhizobacteria (pgpr) viz. pseudomonas argentinensis (lpgpr1), pseudomonas sp. (lpgpr2) along with natio ... | 2015 | 26261403 |
| fermentative hydrogen production from agroindustrial lignocellulosic substrates. | to achieve economically competitive biological hydrogen production, it is crucial to consider inexpensive materials such as lignocellulosic substrate residues derived from agroindustrial activities. it is possible to use (1) lignocellulosic materials without any type of pretreatment, (2) lignocellulosic materials after a pretreatment step, and (3) lignocellulosic materials hydrolysates originating from a pretreatment step followed by enzymatic hydrolysis. according to the current literature data ... | 2015 | 26273246 |
| biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium. | three bacterial isolates identified as alcanivorax borkumensis sk2, rhodococcus erythropolis hs4 and pseudomonas stutzeri sdm, based on 16s rrna gene sequences, were isolated from crude oil enrichments of natural seawater. single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (alcanivorax: pseudomonas, 1:1), (alcanivorax: rhodococcus, 1:1), (pseudomonas: rhodococcus, 1:1), and (alcanivorax: pseudomonas: rhodococcus, 1:1 ... | 2015 | 26273252 |
| treatment of uncomplicated symptomatic urinary tract infections: resistance patterns and misuse of antibiotics. | uncomplicated but symptomatic urinary tract infections (utis) are a common problem seen in practice. the study was undertaken to assess the most common pathogens responsible for uncomplicated symptomatic utis and the antimicrobial resistance pattern in a hospital in bangalore. the study also explores the issue of antibiotic usage for these patients. | 2015 | 26288784 |
| multifarious activities of cellulose degrading bacteria from koala (phascolarctos cinereus) faeces. | cellulose degrading bacteria from koala faeces were isolated using caboxymethylcellulose-congo red agar, screened in vitro for different hydrolytic enzyme activities and phylogenetically characterized using molecular tools. bacillus sp. and pseudomonas sp. were the most prominent bacteria from koala faeces. the isolates demonstrated good xylanase, amylase, lipase, protease, tannase and lignin peroxidase activities apart from endoglucanase activity. furthermore many isolates grew in the presence ... | 2015 | 26290743 |
| biosurfactant production by bacillus salmalaya for lubricating oil solubilization and biodegradation. | this study investigated the capability of a biosurfactant produced by a novel strain of bacillus salmalaya to enhance the biodegradation rates and bioavailability of organic contaminants. the biosurfactant produced by cultured strain 139si showed high physicochemical properties and surface activity in the selected medium. the biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactan ... | 2015 | 26295402 |
| overcoming differences: the catalytic mechanism of metallo-β-lactamases. | metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. the worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. inhibitor design has been mostly impeded by the structural diversity of these enzymes. here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyze ... | 2015 | 26297824 |
| metabolic degradation of 1,4-dichloronaphthalene by pseudomonas sp. hy. | there is increasing concern regarding the adverse health effects of polychlorinated naphthalenes (pcns). the metabolic degradation of 1,4-dichloronaphthalene (1,4-dcn) as a model pcn, was studied using a strain of pseudomonas sp. hy. the metabolites were analyzed by gas chromatography-mass spectrometry (gc-ms). a series of metabolites including dihydroxy-dichloro-naphthalene, epoxy-dichlorinated naphthalene, dichlorinated naphthol, and dichlorinated salicylic acid were identified. the time-conce ... | 2015 | 26308037 |
| brkautodisplay: functional display of multiple exogenous proteins on the surface of escherichia coli by using brka autotransporter. | bacterial surface display technique enables the exogenous proteins or polypeptides displayed on the bacterial surface, while maintaining their relatively independent spatial structures and biological activities. the technique makes recombinant bacteria possess the expectant functions, subsequently, directly used for many applications. many proteins could be used to achieve bacterial surface display, among them, autotransporter, a member of the type v secretion system of gram-negative bacteria, h ... | 2015 | 26337099 |
| live-streaming: time-lapse video evidence of novel streamer formation mechanism and varying viscosity. | time-lapse videos of growing biofilms were analyzed using a background subtraction method, which removed camouflaging effects from the heterogeneous field of view to reveal evidence of streamer formation from optically dense biofilm segments. in addition, quantitative measurements of biofilm velocity and optical density, combined with mathematical modeling, demonstrated that streamer formation occurred from mature, high-viscosity biofilms. we propose a streamer formation mechanism by sudden part ... | 2015 | 26339304 |
| preparation of dental resins resistant to enzymatic and hydrolytic degradation in oral environments. | the short average service life of traditional dental composite restorative materials and increasing occurrence of secondary caries adjacent to composite restorations and sealants are necessitating the development of new, longer lasting compositions. novel monomers and their polymers, reinforcing fillers, and adhesive components are needed. the goal of this research is to develop resin systems for use in restorations, sealants, and other dental services that are superior in properties and enduran ... | 2015 | 26358180 |
| skin-derived c-terminal filaggrin-2 fragments are pseudomonas aeruginosa-directed antimicrobials targeting bacterial replication. | soil- and waterborne bacteria such as pseudomonas aeruginosa are constantly challenging body surfaces. since infections of healthy skin are unexpectedly rare, we hypothesized that the outermost epidermis, the stratum corneum, and sweat glands directly control the growth of p. aeruginosa by surface-provided antimicrobials. due to its high abundance in the upper epidermis and eccrine sweat glands, filaggrin-2 (flg2), a water-insoluble 248 kda s100 fused-type protein, might possess these innate eff ... | 2015 | 26371476 |
| screening the drug resistance property among aerobic pathogenic microorganisms of dental caries in north-western indian population: a preliminary study. | to evaluate the emerging drug resistance among the caries pathogens isolated from carious dentine microbiologically. | 2015 | 26393195 |
| an automated genomes-to-natural products platform (gnp) for the discovery of modular natural products. | bacterial natural products are a diverse and valuable group of small molecules, and genome sequencing indicates that the vast majority remain undiscovered. the prediction of natural product structures from biosynthetic assembly lines can facilitate their discovery, but highly automated, accurate, and integrated systems are required to mine the broad spectrum of sequenced bacterial genomes. here we present a genome-guided natural products discovery tool to automatically predict, combinatorialize ... | 2015 | 26412281 |
| antioxidative, antimicrobial and cytotoxic properties of isatis floribunda boiss. ex bornm. extracts. | the isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of anatolian-turkey. in the present study, the antioxidant activities of isatis floribunda boiss. ex bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. the total phenolic compounds and flavonoids were also examined for ... | 2013 | 26417224 |
| biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes. | release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. thus, in the present study, a bacterial consortium consisting of providencia rettgeri strain hsl1 and pseudomonas sp. suk1 has been investigated for degradation and detoxification of structurally different azo dyes. the consortium showed 98-99 % decolorization of all the selected azo dyes viz. reactive black 5 (rb 5), reactive orange 16 (r ... | 2015 | 26417357 |
| taking a corneal scrape and making a diagnosis. | 2015 | 26435585 | |
| fiber-optic chemical sensors and fiber-optic bio-sensors. | this review summarizes principles and current stage of development of fiber-optic chemical sensors (focs) and biosensors (fobs). fiber optic sensor (fos) systems use the ability of optical fibers (of) to guide the light in the spectral range from ultraviolet (uv) (180 nm) up to middle infrared (ir) (10 μm) and modulation of guided light by the parameters of the surrounding environment of the of core. the introduction of of in the sensor systems has brought advantages such as measurement in flamm ... | 2015 | 26437407 |
| biochemistry and genetics of acc deaminase: a weapon to "stress ethylene" produced in plants. | 1-aminocyclopropane-1-carboxylate deaminase (accd), a pyridoxal phosphate-dependent enzyme, is widespread in diverse bacterial and fungal species. owing to accd activity, certain plant associated bacteria help plant to grow under biotic and abiotic stresses by decreasing the level of "stress ethylene" which is inhibitory to plant growth. accd breaks down acc, an immediate precursor of ethylene, to ammonia and α-ketobutyrate, which can be further metabolized by bacteria for their growth. acc deam ... | 2015 | 26441873 |
| multi-disciplinary antimicrobial strategies for improving orthopaedic implants to prevent prosthetic joint infections in hip and knee. | like any foreign object, orthopaedic implants are susceptible to infection when introduced into the human body. without additional preventative measures, the absolute number of annual prosthetic joint infections will continue to rise, and may exceed the capacity of health care systems in the near future. bacteria are difficult to eradicate from synovial joints due to their exceptionally diverse taxonomy, complex mechanistic attachment capabilities, and tendency to evolve antibiotic resistance. w ... | 2015 | 26449208 |
| multi-disciplinary antimicrobial strategies for improving orthopaedic implants to prevent prosthetic joint infections in hip and knee. | like any foreign object, orthopaedic implants are susceptible to infection when introduced into the human body. without additional preventative measures, the absolute number of annual prosthetic joint infections will continue to rise, and may exceed the capacity of health care systems in the near future. bacteria are difficult to eradicate from synovial joints due to their exceptionally diverse taxonomy, complex mechanistic attachment capabilities, and tendency to evolve antibiotic resistance. w ... | 2015 | 26449208 |
| the 29(th) annual symposium of the protein society, barcelona, spain, july 22-25, 2015. | 2015 | 26452528 | |
| the genomic landscape of ribosomal peptides containing thiazole and oxazole heterocycles. | ribosomally synthesized and post-translationally modified peptides (ripps) are a burgeoning class of natural products with diverse activity that share a similar origin and common features in their biosynthetic pathways. the precursor peptides of these natural products are ribosomally produced, upon which a combination of modification enzymes installs diverse functional groups. this genetically encoded peptide-based strategy allows for rapid diversification of these natural products by mutation i ... | 2015 | 26462797 |
| a novel eurythermic and thermostale lipase lipm from pseudomonas moraviensis m9 and its application in the partial hydrolysis of algal oil. | lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. microbial lipases in particular have been widely used in a variety of industrial applications. however, the current commercial microbial lipases cannot meet industrial demand due to rapid inactivation under harsh conditions. therefore, in order to identify more stable enzymes, we isolated novel eurythermic and thermostable lipase(s) from pseudomonas moraviensis m9. | 2015 | 26463643 |
| mechanism of aromatic amine carcinogen bypass by the y-family polymerase, dpo4. | bulky dna damage inhibits dna synthesis by replicative polymerases and often requires the action of error prone bypass polymerases. the exact mechanism governing adduct-induced mutagenesis and its dependence on the dna sequence context remains unclear. in this work, we characterize dpo4 binding conformations and activity with dna templates modified with the carcinogenic dna adducts, 2-aminofluoene (af) or n-acetyl-2-aminofluorene (aaf), using single-molecule fret (smfret) analysis and dna synthe ... | 2015 | 26481355 |
| biodegradation kinetics and interactions of styrene and ethylbenzene as single and dual substrates for a mixed bacterial culture. | this study examined biodegradation kinetics of styrene and ethylbenzene as representatives of alkenylbenzenes and mono-alkylbenzenes, respectively. the compounds were studied independently and as binary mixtures using a series of aerobic batch degradation experiments introduced by acclimatized mix culture. initial concentration of styrene and ethylbenzene in the liquid phase vacillated from 0 to 220 mg/l. the andrew model was applied for the biodegradation of individual substrates and the estima ... | 2015 | 26483955 |
| additivity of the stabilization effect of single amino acid substitutions in triple mutants of recombinant formate dehydrogenase from the soybean glycine max. | recently, we demonstrated that the amino acid substitutions ala267met and ala267met/ile272val (alekseeva et al., biochemistry, 2012), phe290asp, phe290asn and phe290ser (alekseeva et al., prot. eng. des. select, 2012) in recombinant formate dehydrogenase from soya glycine max (soyfdh) lead to a significant (up to 30-100 times) increase in the thermal stability of the enzyme. the substitutions phe290asp, phe290asn and phe290ser were introduced into double mutant soyfdh ala267met/ile272val by site ... | 2015 | 26483960 |
| port site infection in laparoscopic surgery: a review of its management. | laparoscopic surgery (ls), also termed minimal access surgery, has brought a paradigm shift in the approach to modern surgical care. early postoperative recovery, less pain, improved aesthesis and early return to work have led to its popularity both amongst surgeons and patients. its application has progressed from cholecystectomies and appendectomies to various other fields including gastrointestinal surgery, urology, gynecology and oncosurgery. however, ls has its own package of complications. ... | 2015 | 26488021 |
| structure analysis uncovers a highly diverse but structurally conserved effector family in phytopathogenic fungi. | phytopathogenic ascomycete fungi possess huge effector repertoires that are dominated by hundreds of sequence-unrelated small secreted proteins. the molecular function of these effectors and the evolutionary mechanisms that generate this tremendous number of singleton genes are largely unknown. to get a deeper understanding of fungal effectors, we determined by nmr spectroscopy the 3-dimensional structures of the magnaporthe oryzae effectors avr1-co39 and avr-pia. despite a lack of sequence simi ... | 2015 | 26506000 |
| transcriptome analysis of campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants. | 2015 | 26537695 | |
| evaluation of antimicrobial therapy of blood culture positive healthcare-associated infections in children. | knowledge of the quality of antimicrobial therapy (amt) used for invasive healthcare-associated infections (hais) in paediatrics is scarce. influence of the final information about the isolated pathogen on the subsequent targeted amt was investigated in our study. | 2015 | 26539831 |
| ultrasound scan as a potential source of nosocomial and crossinfection: a literature review. | the authors review the main concepts regarding the importance of cleaning/disinfection of ultrasonography probes, aiming a better comprehension by practitioners and thus enabling strategies to establish a safe practice without compromising the quality of the examination and the operator productivity. in the context of biosafety, it is imperative to assume that contact with blood or body fluids represents a potential source of infection. thus, in order to implement cleaning/disinfection practice, ... | 2015 | 26543284 |
| psychrophilic pseudomonas in antarctic freshwater lake at stornes peninsula, larsemann hills over east antarctica. | the larsemann hills is an ice-free area of approximately 50 km(2), located halfway between the vestfold hills and the amery ice shelf on the south-eastern coast of prydz bay, princess elizabeth land, east antarctica (69º30's, 76º19'58″e). the ice-free area consists of two major peninsulas (stornes and broknes), four minor peninsulas, and approximately 130 islands. the larsemann hills area contains more than 150 lakes at different islands and peninsulas. nine lake water samples were collected in ... | 2015 | 26543717 |
| a fragrant neighborhood: volatile mediated bacterial interactions in soil. | there is increasing evidence that volatile organic compounds (vocs) play essential roles in communication and competition between soil microorganisms. here we assessed volatile-mediated interactions of a synthetic microbial community in a model system that mimics the natural conditions in the heterogeneous soil environment along the rhizosphere. phylogenetic different soil bacterial isolates (burkholderia sp., dyella sp., janthinobacterium sp., pseudomonas sp., and paenibacillus sp.) were inocul ... | 2015 | 26579111 |
| high potential application in bioremediation of selenate by proteus hauseri strain qw4. | selenium is essential for biological systems at low concentrations and toxic at higher levels. heavy metals and metalloids such as selenium are major contaminants in 40% of hazardous waste sites. thus, bioremediation has been considered as an effective means of cleaning up of selenium-contaminated sites. | 2015 | 26622970 |
| composition of the spruce budworm (choristoneura fumiferana) midgut microbiota as affected by rearing conditions. | the eastern spruce budworm (choristoneura fumiferana) is one of the most destructive forest insect pests in canada. little is known about its intestinal microbiota, which could play a role in digestion, immune protection, communication and/or development. the present study was designed to provide a first characterization of the effects of rearing conditions on the taxonomic diversity and structure of the c. fumiferana midgut microbiota, using a culture-independent approach. three diets and insec ... | 2015 | 26636571 |
| comparative genomics of pseudomonas fluorescens subclade iii strains from human lungs. | while the taxonomy and genomics of environmental strains from the p. fluorescens species-complex has been reported, little is known about p. fluorescens strains from clinical samples. in this report, we provide the first genomic analysis of p. fluorescens strains in which human vs. environmental isolates are compared. | 2015 | 26644001 |
| p accumulation and physiological responses to different high p regimes in polygonum hydropiper for understanding a p-phytoremediation strategy. | phosphorus (p) accumulators used for phytoremediation vary in their potential to acquire p from different high p regimes. growth and p accumulation in polygonum hydropiper were both dependent on an increasing level of ihp (1-8 mm p) and on a prolonged growth period (3-9 weeks), and those of the mining ecotype (me) were higher than the non-mining ecotype (nme). biomass increments in root, stem, and leaf of both ecotypes were significantly greater in ihp relative to other organic p (po) sources (g ... | 2015 | 26648137 |
| phylogenetic analysis based evolutionary study of 16s rrna in known pseudomonas sp. | molecular evolution analysis of 16s rrna sequences of native pseudomonas strains and different fluorescent pseudomonads were conducted on the basis of molecular evolutionary genetics analysis version 5.2 (mega5.2). topological evaluations show common origin for native strains with other known strains with available sequences at genbank database. phylogenetic affiliation of different pseudomonas sp based on 16s rrna gene shows that molecular divergence contributes to the genetic diversity of pseu ... | 2015 | 26664032 |
| immobilization of microbes for bioremediation of crude oil polluted environments: a mini review. | petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. oil pollution may arise either accidentally or operationally whenever oil is produced, transported, stored and processed or used at sea or on land. oil spills are a major menace to the environment as they severely damage the surrounding ecosystems. to improve the survival and retention of the bioremediation agents in the contaminated sites, bac ... | 2015 | 26668662 |
| ultrasound assessment of thrombotic complications in pediatric patients with tunneled central venous catheters. | central venous access consists in inserting a vascular catheter to the vena cava and placing its tip in the vicinity of the opening to the right atrium. in the patients of the clinic of pediatrics, hematology and oncology at the academic clinical centre of the medical university in gdańsk, such implantation procedures are conducted 40-50 times in a year using broviac/hickman catheters that are placed in the subclavian vein. in the ultrasound and biopsy laboratory at the clinic mentioned above, a ... | 2013 | 26673005 |
| mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa. | bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ... | 2016 | 26712005 |
| mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa. | bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ... | 2016 | 26712005 |
| a quick and robust method for quantification of the hypersensitive response in plants. | one of the most studied defense reactions of plants against microbial pathogens is the hypersensitive response (hr). the hr is a complex multicellular process that involves programmed cell death at the site of infection. a standard method to quantify plant defense and the hr is to measure the release of cellular electrolytes into water after infiltration with pathogenic bacteria. in this type of experiment, the bacteria are typically delivered into the plant tissue through syringe infiltration. ... | 2015 | 26734506 |
| bioactivities by a crude extract from the greenlandic pseudomonas sp. in5 involves the nonribosomal peptides, nunamycin and nunapeptin. | background. bioactive microbial metabolites provide a successful source of novel compounds with pharmaceutical potentials. the bacterium pseudomonas sp. in5 is a biocontrol strain isolated from a plant disease suppressive soil in greenland, which produces two antimicrobial nonribosomal peptides (nrps), nunapeptin and nunamycin. methods. in this study, we used in vitro antimicrobial and anticancer bioassays to evaluate the potential bioactivities of both a crude extract derived from pseudomonas s ... | 2015 | 26734508 |
| cross-regulation between the phz1 and phz2 operons maintain a balanced level of phenazine biosynthesis in pseudomonas aeruginosa pao1. | gene duplication often provides selective advantages for the survival of microorganisms in adapting to varying environmental conditions. p. aeruginosa pao1 possesses two seven-gene operons [phz1 (phza1b1c1d1e1f1g1) and phz2 (phza2b2c2d2e2f2g2)] that are involved in the biosynthesis of phenazine-1-carboxylic acid and its derivatives. although the two operons are highly homologous and their functions are well known, it is unclear how the two phz operons coordinate their expressions to maintain the ... | 2016 | 26735915 |
| a lipid-accumulating alga maintains growth in outdoor, alkaliphilic raceway pond with mixed microbial communities. | algal biofuels and valuable co-products are being produced in both open and closed cultivation systems. growing algae in open pond systems may be a more economical alternative, but this approach allows environmental microorganisms to colonize the pond and potentially infect or outcompete the algal "crop." in this study, we monitored the microbial community of an outdoor, open raceway pond inoculated with a high lipid-producing alkaliphilic alga, chlorella vulgaris ba050. the strain c. vulgaris b ... | 2015 | 26779138 |
| a lipid-accumulating alga maintains growth in outdoor, alkaliphilic raceway pond with mixed microbial communities. | algal biofuels and valuable co-products are being produced in both open and closed cultivation systems. growing algae in open pond systems may be a more economical alternative, but this approach allows environmental microorganisms to colonize the pond and potentially infect or outcompete the algal "crop." in this study, we monitored the microbial community of an outdoor, open raceway pond inoculated with a high lipid-producing alkaliphilic alga, chlorella vulgaris ba050. the strain c. vulgaris b ... | 2015 | 26779138 |
| a novel mva-mediated pathway for isoprene production in engineered e. coli. | to deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel. | 2016 | 26786050 |
| foliar endophytic fungi as potential protectors from pathogens in myrmecophytic acacia plants. | in defensive ant-plant interactions myrmecophytic plants express reduced chemical defense in their leaves to protect themselves from pathogens, and it seems that mutualistic partners are required to make up for this lack of defensive function. previously, we reported that mutualistic ants confer plants of acacia hindsii protection from pathogens, and that the protection is given by the ant-associated bacteria. here, we examined whether foliar endophytic fungi may potentially act as a new partner ... | 2014 | 26843901 |
| recent developments in using advanced sequencing technologies for the genomic studies of lignin and cellulose degrading microorganisms. | lignin is a complex polyphenyl aromatic compound which exists in tight associations with cellulose and hemicellulose to form plant primary and secondary cell wall. lignocellulose is an abundant renewable biomaterial present on the earth. it has gained much attention in the scientific community in recent years because of its potential applications in bio-based industries. microbial degradation of lignocellulose polymers was well studied in wood decaying fungi. based on the plant materials they de ... | 2016 | 26884714 |
| analysis for the presence of determinants involved in the transport of mercury across bacterial membrane from polluted water bodies of india. | mercury, which is ubiquitous and recalcitrant to biodegradation processes, threatens human health by escaping to the environment via various natural and anthropogenic activities. non-biodegradability of mercury pollutants has necessitated the development and implementation of economic alternatives with promising potential to remove metals from the environment. enhancement of microbial based remediation strategies through genetic engineering approaches provides one such alternative with a promisi ... | 2016 | 26887227 |
| bioaugmentation mitigates the impact of estrogen on coliform-grazing protozoa in slow sand filters. | exposure to endocrine-disrupting chemicals (edcs), such as estrogens, is a growing issue for human and animal health as they have been shown to cause reproductive and developmental abnormalities in wildlife and plants and have been linked to male infertility disorders in humans. intensive farming and weather events, such as storms, flash flooding, and landslides, contribute estrogen to waterways used to supply drinking water. this paper explores the impact of estrogen exposure on the performance ... | 2016 | 26895622 |
| genetic and functional analysis of the biosynthesis of a non-ribosomal peptide siderophore in burkholderia xenovorans lb400. | b. xenovorans lb400 is a model bacterium for the study of the metabolism of aromatic compounds. the aim of this study was the genomic and functional characterization of a non-ribosomal peptide synthetase containing gene cluster that encodes a siderophore in b. xenovorans lb400. the mba gene cluster from strain lb400 encodes proteins involved in the biosynthesis and transport of a hydroxamate-type siderophore. strain lb400 has a unique mba gene organization, although mba gene clusters have been o ... | 2016 | 26963250 |
| highly stable and reusable immobilized formate dehydrogenases: promising biocatalysts for in situ regeneration of nadh. | this study aimed to prepare robust immobilized formate dehydrogenase (fdh) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of nadh is required. for this purpose, candida methylica fdh was covalently immobilized onto immobead 150 support (fdhi150), immobead 150 support modified with ethylenediamine and then activated with glutaraldehyde (fdhiglu), and immobead 150 support functionalized with aldehyde groups (fdhiald). t ... | 2016 | 26977186 |
| response and resilience of soil microbial communities inhabiting in edible oil stress/contamination from industrial estates. | gauging the microbial community structures and functions become imperative to understand the ecological processes. to understand the impact of long-term oil contamination on microbial community structure soil samples were taken from oil fields located in different industrial regions across kadi, near ahmedabad, india. soil collected was hence used for metagenomic dna extraction to study the capabilities of intrinsic microbial community in tolerating the oil perturbation. | 2016 | 27001503 |
| temperature and redox effect on mineral colonization in juan de fuca ridge flank subsurface crustal fluids. | to examine microbe-mineral interactions in subsurface oceanic crust, we evaluated microbial colonization on crustal minerals that were incubated in borehole fluids for 1 year at the seafloor wellhead of a crustal borehole observatory (iodp hole u1301a, juan de fuca ridge flank) as compared to an experiment that was not exposed to subsurface crustal fluids (at nearby iodp hole u1301b). in comparison to previous studies at these same sites, this approach allowed assessment of the effects of temper ... | 2016 | 27064928 |
| biosynthesis, chemical structure, and structure-activity relationship of orfamide lipopeptides produced by pseudomonas protegens and related species. | orfamide-type cyclic lipopeptides (clps) are biosurfactants produced by pseudomonas and involved in lysis of oomycete zoospores, biocontrol of rhizoctonia and insecticidal activity against aphids. in this study, we compared the biosynthesis, structural diversity, in vitro and in planta activities of orfamides produced by rhizosphere-derived pseudomonas protegens and related pseudomonas species. genetic characterization together with chemical identification revealed that the main orfamide compoun ... | 2016 | 27065956 |
| achromobacter xylosoxidans as a new microorganism strain colonizing high-density polyethylene as a key step to its biodegradation. | this study presents results of research on isolation new bacteria strain achromobacter xylosoxidans able to effect on the structure of high-density polyethylene (hdpe), polymer resistant to degradation in environment. new strain of a. xylosoxidans pe-1 was isolated from the soil and identified by analysis of the 16s ribosome subunit coding sequences. the substance to be degraded was hdpe in the form of thin foil films. the foil samples were analyzed with attenuated total reflectance fourier tran ... | 2016 | 27072033 |
| pharmacological potentials of premna integrifolia l. | premna integrifolia linn. (verbenaceae) is an important constituent of the formulation of ten roots of herbs known as daśamūla and is widely used for treating various ailments in the indian system of medicine. aim of this review is to provide comprehensive information on the pharmacological activities of various parts of p. integrifolia. all the relevant universally accepted electronic databases were searched with respect to the terms "agnimanthā", "headache tree", "premna integrifolia", "premna ... | 2016 | 27143797 |
| donor site morbidity following harvest of split-thickness skin grafts in south eastern nigeria. | split-thickness skin grafting is a very common procedure. few documentation of its complications exist in the literatures especially in our sub-region where excessive scarring is a major issue. | 2011 | 27182501 |
| evidence for the induction of key components of the notch signaling pathway via deltamethrin and azamethiphos treatment in the sea louse caligus rogercresseyi. | the extensive use of organophosphates and pyrethroids in the aquaculture industry has negatively impacted parasite sensitivity to the delousing effects of these antiparasitics, especially among sea lice species. the notch signaling pathway is a positive regulator of abc transporter subfamily c expression and plays a key role in the generation and modulation of pesticide resistance. however, little is known about the molecular mechanisms behind pesticide resistance, partly due to the lack of geno ... | 2016 | 27187362 |
| characterisation of candida within the mycobiome/microbiome of the lower respiratory tract of icu patients. | whether the presence of candida spp. in lower respiratory tract (lrt) secretions is a marker of underlying disease, intensive care unit (icu) treatment and antibiotic therapy or contributes to poor clinical outcome is unclear. we investigated healthy controls, patients with proposed risk factors for candida growth in lrt (antibiotic therapy, icu treatment with and without antibiotic therapy), icu patients with pneumonia and antibiotic therapy and candidemic patients (for comparison of truly inva ... | 2016 | 27206014 |
| antagonistic control of the turnover pathway for the global regulatory srna csrb by the csra and csrd proteins. | the widely conserved protein csra (carbon storage regulator a) globally regulates bacterial gene expression at the post-transcriptional level. in many species, csra activity is governed by untranslated srnas, csrb and csrc in escherichia coli, which bind to multiple csra dimers, sequestering them from lower affinity mrna targets. both the synthesis and turnover of csrb/c are regulated. their turnover requires the housekeeping endonuclease rnase e and is activated by the presence of a preferred c ... | 2016 | 27235416 |
| abstracts. | 2016 | 27243148 | |
| enzyme biosensors for biomedical applications: strategies for safeguarding analytical performances in biological fluids. | enzyme-based chemical biosensors are based on biological recognition. in order to operate, the enzymes must be available to catalyze a specific biochemical reaction and be stable under the normal operating conditions of the biosensor. design of biosensors is based on knowledge about the target analyte, as well as the complexity of the matrix in which the analyte has to be quantified. this article reviews the problems resulting from the interaction of enzyme-based amperometric biosensors with com ... | 2016 | 27249001 |
| bacteria with phosphate solubilizing capacity alter mycorrhizal fungal growth both inside and outside the root and in the presence of native microbial communities. | arbuscular mycorrhizal fungi (amf) and phosphate solubilizing pseudomonas bacteria (psb) could potentially interact synergistically because psb solubilize phosphate into a form that amf can absorb and transport to the plant. however, very little is known about the interactions between these two groups of microorganisms and how they influence the growth of each other. we tested whether different strains of bacteria, that have the capacity to solubilize phosphate, are able to grow along amf hyphae ... | 2016 | 27253975 |
| silica ecosystem for synergistic biotransformation. | synergistical bacterial species can perform more varied and complex transformations of chemical substances than either species alone, but this is rarely used commercially because of technical difficulties in maintaining mixed cultures. typical problems with mixed cultures on scale are unrestrained growth of one bacterium, which leads to suboptimal population ratios, and lack of control over bacterial spatial distribution, which leads to inefficient substrate transport. to address these issues, w ... | 2016 | 27264916 |
| exploring the mechanisms of action of human secretory rnase 3 and rnase 7 against candida albicans. | human antimicrobial rnases, which belong to the vertebrate rnase a superfamily and are secreted upon infection, display a wide spectrum of antipathogen activities. in this work, we examined the antifungal activity of the eosinophil rnase 3 and the skin-derived rnase 7, two proteins expressed by innate cell types that are directly involved in the host defense against fungal infection. candida albicans has been selected as a suitable working model for testing rnase activities toward a eukaryotic p ... | 2016 | 27277554 |
| isolation of bdellovibrio sp. from soil samples in mexico and their potential applications in control of pathogens. | in this study, two strains of bdellovibrio were isolated from soil samples using the culture-dependent technique and two members of the family enterobacteriaceae (klebsiella sp. and salmonella sp.) as prey. the bdellovibrio strains were bacteriolytic, plaque-forming, and highly motile gram-negative bacteria. we identified and confirmed the bdellovibrio strains using microscopy, pcr amplification, and sequencing of the 16s rrna gene. they were observed to be different strains based on hit locus a ... | 2016 | 27297185 |
| nosocomial urinary tract infections caused by extended-spectrum beta-lactamase uropathogens: prevalence, pathogens, risk factors, and strategies for infection control. | our goal was to investigate the prevalence and antibiogram pattern of extended spectrum beta-lactamase (esbl) production among uropathogens using isolates from urine samples collected at the department of urology in the sahloul hospital, tunisia we also aimed to identify the risk factors for nosocomial urinary tract infections (utis) in patients who underwent transurethral resection of the prostate (turp) and the measures for infection control. | 2016 | 27330585 |
| ventricular assist devices as bridge to heart transplantation: impact on post-transplant infections. | ventricular assist devices (vad) are valuable options for patients with heart failure awaiting cardiac transplantation. we assessed the impact of pre-transplant vad implantation on the incidence of post-transplant infections in a nationwide cohort of heart transplant recipients. | 2016 | 27391967 |
| risk factors for fecal carriage of carbapenemase producing enterobacteriaceae among intensive care unit patients from a tertiary care center in india. | resistance amongst the commensal flora is a serious threat because a very highly populated ecosystem like the gut, may at a later stage, be a source of extra intestinal infections, resistant strains may spread to other host or transfer genetic resistance element to other members of micro-biota including pathogens. this study was carried out to assess fecal colonization by carbapenemase producing enterobacteriaceae (cpe) and associated risk factors among 100 patients admitted to intensive care un ... | 2016 | 27392139 |
| human thanatomicrobiome succession and time since death. | the thanatomicrobiome (thanatos, greek for death) is a relatively new term and is the study of the microbes colonizing the internal organs and orifices after death. recent scientific breakthroughs in an initial study of the thanatomicrobiome have revealed that a majority of the microbes within the human body are the obligate anaerobes, clostridium spp., in the internal postmortem microbial communities. we hypothesized that time-dependent changes in the thanatomicrobiome within internal organs ca ... | 2016 | 27412051 |
| functional exploration of the polysaccharide lyase family pl6. | alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (m-residues) and, its c5-epimer, guluronic acid (g-residues). alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by β-elimination. they are classified according to their ability to recognize the distribution of m- and g-residues and are named m-, g- or mg-lyases. in the cazy database, alginate lyases have been grouped by sequence similarity into seven distin ... | 2016 | 27438604 |
| insights into the biodegradation of weathered hydrocarbons in contaminated soils by bioaugmentation and nutrient stimulation. | the potential for biotransformation of weathered hydrocarbon residues in soils collected from two commercial oil refinery sites (soil a and b) was studied in microcosm experiments. soil a has previously been subjected to on-site bioremediation and it was believed that no further degradation was possible while soil b has not been subjected to any treatment. a number of amendment strategies including bioaugmentation with hydrocarbon degrader, biostimulation with nutrients and soil grinding, were a ... | 2016 | 27441989 |
| marine natural product inhibitors of neutrophil-associated inflammation. | neutrophils are widely recognized to play an important role in acute inflammatory responses, and recent evidence has expanded their role to modulating chronic inflammatory and autoimmune diseases. reactive oxygen species (ros) and microbicidal compounds released from neutrophils that are recruited to the site of inflammation contribute to the pathogenesis of multiple inflammation-associated diseases such as chronic obstructive pulmonary disease, atherosclerosis, and hepatitis. marine organisms a ... | 2016 | 27472345 |
| analysis of plant growth-promoting effects of fluorescent pseudomonas strains isolated from mentha piperita rhizosphere and effects of their volatile organic compounds on essential oil composition. | many species or strains of the genus pseudomonas have been characterized as plant growth promoting rhizobacteria (pgpr). we used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent pseudomonas strains in the rhizosphere of commercially grown mentha piperita (peppermint). biochemical techniques, amplified rdna restriction analysis (ardra), and 16s rrna gene sequence analysis revealed that the majority of the isolated native fluorescent strains were p. puti ... | 2016 | 27486441 |
| molecular cloning and functional expression of a δ9- fatty acid desaturase from an antarctic pseudomonas sp. a3. | fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. pseudomonas sp. a3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. using polymerase chain reaction (pcr), a novel δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in escherichia coli. the gene was designated as pa3fad9 and has an open reading frame of 1,185 bp which codes for 394 amino acids with a predicted mole ... | 2016 | 27494717 |
| use of ferrous iron by metallo-β-lactamases. | metallo-β-lactamases (mbls) catalyse the hydrolysis of almost all β-lactam antibacterials including the latest generation carbapenems and are a growing worldwide clinical problem. it is proposed that mbls employ one or two zinc ion cofactors in vivo. isolated mbls are reported to use transition metal ions other than zinc, including copper, cadmium and manganese, with iron ions being a notable exception. we report kinetic and biophysical studies with the di-iron(ii)-substituted metallo-β-lactamas ... | 2016 | 27498591 |
| fabrication of unique magnetic bionanocomposite for highly efficient removal of hexavalent chromium from water. | biotreatment of hexavalent chromium has attracted widespread interest due to its cost effective and environmental friendliness. however, the difficult separation of biomass from aqueous solution and the slow hexavalent chromium bioreduction rate are bottlenecks for biotechnology application. in this approach, a core-shell structured functional polymer coated magnetic nanocomposite was prepared for enriching the hexavalent chromium. then the nanocomposite was connected to the bacteria via amines ... | 2016 | 27502074 |
| 16s rrna amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae. | data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. at 6 days postfertilization (dpf), these "conventionalized" larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization proces ... | 2016 | 27508247 |
| enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage. | copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (sossego mine, canaã dos carajás, brazil). hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. bacterial s ... | 2016 | 27522533 |
| microbiota in breast milk of chinese lactating mothers. | the microbiota of breast milk from chinese lactating mothers at different stages of lactation was examined in the framework of a maternal infant nutrition growth (ming) study investigating the dietary habits and breast milk composition in chinese urban mothers. we used microbiota profiling based on the sequencing of fragments of 16s rrna gene and specific qpcr for bifidobacteria, lactobacilli and total bacteria to study microbiota of the entire breast milk collected using standard protocol witho ... | 2016 | 27529821 |