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identification of the promoter of the pseudomonas gene coding for carboxypeptidase g2.a 213-bp region of noncoding dna upstream of the atg start codon of the pseudomonas carboxypeptidase g2 (cpg2) structural gene has been shown to contain the cpg2 promoter. the mrna start point (+1) on the dna sequence has been identified by mapping the 5' end of the cpg2 transcript. the identified promoter region contains a -10 region (tataag) that closely resembles the escherichia coli consensus sequence (tataat), but has no easily recognisable -35 region. the lack of homology in the -35 region ...19853839252
periplasmic location of p-cresol methylhydroxylase in pseudomonas putida.the cellular location of the flavocytochrome c, p-cresol methylhydroxylase was investigated in two strains of pseudomonas putida. in both cases the enzymes were shown to be located in the periplasmic fraction by their release during treatment of the bacteria with edta and lysozyme in a solution containing a high concentration of sucrose. for strain ncib 9869 the finding is in accord with the suggestion that the physiological acceptor for the enzyme is azurin as this too was shown to be located m ...19853920077
chromosomal map of pseudomonas putida ppn, and a comparison of gene order with the pseudomonas aeruginosa pao chromosomal map.the generalized transducing phage pf16h2 has been used to confirm linkage relationships of chromosomal markers of pseudomonas putida previously determined from their time-of-entry in hfr crosses, and to map new auxotrophic mutations. by means of spot matings using hfr donors of known origin of transfer, catabolic markers forming part of a closely linked group of operons referred to as a superoperonic cluster have been shown to be chromosomally located and their map positions determined. r-prime- ...19853921659
anti-pseudomonal activity of hr 810.the anti-pseudomonal activity of hr 810, a new 2-aminothiazolyl cephalosporin, was compared to that of carbenicillin, azlocillin and cefsulodin against 187 non-fermenters. hr 810 was the best agent against pseudomonas aeruginosa, pseudomonas putida, pseudomonas fluorescens and acinetobacter calcoaceticus with an mic50 less than or equal to 4 mg/l and an mic90 less than or equal to 16 mg/l. it was as effective as azlocillin against pseudomonas stutzeri and pseudomonas mendocina, with an mic50 les ...19853922898
cloning of genes specifying carbohydrate catabolism in pseudomonas aeruginosa and pseudomonas putida.a 6.0-kilobase ecori fragment of the pseudomonas aeruginosa pao chromosome containing a cluster of genes specifying carbohydrate catabolism was cloned into the multicopy plasmid pro1769. the vector contains a unique ecori site for cloning within a streptomycin resistance determinant and a selectable gene encoding gentamicin resistance. mutants of p. aeruginosa pao transformed with the chimeric plasmid pro1816 regained the ability to grow on glucose, and the following deficiencies in enzyme or tr ...19853922954
volatiles of pseudomonas aeruginosa and related species by automated headspace concentration--gas chromatography.the volatile metabolites of three strains of pseudomonas aeruginosa and one strain each of pseudomonas cepacia, pseudomonas maltophilia, pseudomonas fluorescens, and pseudomonas putida were analyzed using an automated headspace concentrator incorporating a gas chromatograph. the procedure does not require sample preparation and automates the entire analytical sequence to yield reproducible profiles of volatile constituents. gas chromatographic profiles of the volatile metabolites of each species ...19853924382
genes specifying degradation of 3-chlorobenzoic acid in plasmids pac27 and pjp4.all of the structural genes for 3-chlorobenzoate degradation are clustered in a 4.2-kilobase (kb) region of plasmid pac25 (or pac27) in pseudomonas putida. an approximate 10-kb dna segment containing three structural genes for chlorocatechol metabolism present on plasmid pjp4 in alcaligenes eutrophus shows homology with the above 4.2-kb region of pac27. in spite of the detectable sequence homology in the structural genes present on both plasmids, the regulation of their expression seems quite di ...19853856842
transcriptional control of the nah and sal hydrocarbon-degradation operons by the nahr gene product.the positively regulated nah and sal operons of the nah7 plasmid from pseudomonas putida encode the enzymes for metabolism of naphthalene via salicylate. to study their coordinate regulation, a 6-kb dna fragment containing the entire naha gene (encoding naphthalene dioxygenase), the gene of the nah operon, was cloned into a rsf1010 plasmid derivative. analysis of expression of naha from the nah promoter in either escherichia coli or pseudomonas putida showed that a 1.6-kb dna fragment from the n ...19853908220
presence and quantity of dehydroalanine in histidine ammonia-lyase from pseudomonas putida.dehydroalanine is present in the histidine ammonia-lyase (histidase) from pseudomonas putida atcc 12633 as shown by reaction of purified enzyme with k14cn or nab3h4 and subsequent identification of [14c]aspartate or [3h]alanine, respectively, following acid hydrolysis of the labeled protein. when labeling with cyanide was conducted under denaturing conditions, 4 mol of [14c]cyanide was incorporated per mol of enzyme (mr 220 000), equivalent to one dehydroalanine residue being modified per subuni ...19853919759
[regulation of the synthesis of the key enzymes for naphthalene catabolism in pseudomonas putida and pseudomonas fluorescens carrying the biodegradation plasmids nah, pbs3, pbs2 and npl-1].regulation of the synthesis of key enzymes catalysing naphthalene catabolism was studied in pseudomonas strains containing different plasmids of naphthalene biodegradation. the synthesis of naphthalene oxygenase, salicylate hydroxylase, catechol-1,2-oxygenase and cathechol-2,3-oxygenase was shown to be regulated in both the coordinated and non-coordinated manner.19853937034
effect of carbon dioxide on growth of pseudomonas putida atcc 11172 on asparagine, citrate, glucose, and lactate in batch and continuous culture.the growth of pseudomonas putida atcc 11172 on l-asparagine, citrate, d-glucose, and l-lactate was followed in air and in 40% co2 + air, using batch and carbon-limited continuous cultures. batch cultures in air utilized a mixture of the carbon sources simultaneously. however, a change to 40% co2 favoured the utilization of glucose. the maximum specific growth rate (mumax) in air was about 0.3 h-1 on glucose and 0.6 h-1 on the other carbon sources. in co2, the mumax for glucose was reduced by 16% ...19853936609
plasmid-determined cadmium resistance in pseudomonas putida gam-1 isolated from soil.cadmium-resistant pseudomonas putida gam-1, which was able to grow in concentrations of cdcl2 as high as 7 mm, was isolated from soil in a rice paddy. this bacterium harbored a dna plasmid of about 52 kilobases. the plasmid (pgu100) transformed escherichia coli c600 to cadmium resistance. a cadmium-resistant transformant of e. coli c600 contained a plasmid corresponding to that seen in p. putida gam-1. the transformant did not take up cadmium as well as p. putida gam-1 did.19863941050
camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from pseudomonas putida atcc 17453.the oxygenating component of 2,5-diketocamphane 1,2-monooxygenase from pseudomonas putida atcc 17453 was purified to homogeneity by a combination of ammonium sulfate fractionation and chromatography on deae-cellulose and polyanion si-17 columns. it had an mr of 78,000, bound one molecule of nonautooxidizable flavin mononucleotide (fmn), consisted of two subunits of equal molecular weight, and existed in two electrophoretically distinguishable active forms. the oxygenating complex was constructed ...19863944058
[effective method of transduction with virulent phage pf16 using specific mutants of pseudomonas putida ppg1].a procedure of simple selection of pseudomonas putida ppg1 mutants is described. the mutants can be used for transduction with virulent pf16 phage, giving reliable results. the frequency of transduction of chromosomal markers ilv and trp was 10(-6). also, transduction of plasmid rp4 with phage pf16 was shown with the frequency of 10(-7).19853926609
measuring the spermosphere colonizing capacity (spermosphere competence) of bacterial inoculants.spermosphere establishment by bacteria which were coated onto seeds was studied using soybean seeds treated with four bacterial strains at levels of log10 1 to 4 colony-forming units (cfu) per seed planted in a field soil mix, and incubated 48 h. each strain at every inoculum level developed spermosphere population densities of log10 4 to 8 cfu/seed, demonstrating an average multiplication of log10 3 cfu/seed. an alternative method was developed to differentially rank bacteria for spermosphere c ...19853933804
immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria.purified catabolic ornithine carbamoyltransferase of pseudomonas putida and anabolic ornithine carbamoyltransferase (argf product) of escherichia coli k-12 were used to prepare antisera. the two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by pseudomonas and representative organisms of other bacterial genera. the immunological cross-reactivity observed only between the catabolic ornithine carbamoyltrans ...19853968036
[formaldehyde resistance to gram-negative aerobic rods from municipal sewage water].in a municipal sewage works, a total of 30 sewage samples (19 from the inlet and 11 from the outlet of the sewage works) were analyzed for the quantitative and orientative qualitative content of microorganisms. with an incidence peak of the total bacterial count of greater than or equal to 1 x 10(6) cfu/ml in the inlet, both samples showed bacterial contents of 1 to 9 x 10(5) cfu/ml with use of mc agar and endo agar. fuchsin glistening colonies as well as the total bacterial counts on sabouraud ...19853939051
conjugative mapping of pyruvate, 2-ketoglutarate, and branched-chain keto acid dehydrogenase genes in pseudomonas putida mutants.branched-chain keto acid dehydrogenase, an enzyme in the common pathway of branched-chain amino acid catabolism of pseudomonas putida, is a multienzyme complex which catalyzes the oxidative decarboxylation of branched-chain keto acids. the objective of the present study was to isolate strains with mutations of this and other keto acid dehydrogenases and to map the location of the mutations on the chromosome of p. putida. several strains with mutations of branched-chain keto acid dehydrogenase, t ...19853980435
beta-casomorphin immunoreactive materials in cows' milk incubated with various bacterial species.to obtain information about the possible release of beta-casomorphins from beta-casein under in vitro conditions, cows' milk was incubated with 13 strains of gram-negative or gram-positive bacterial species isolated from bovine milk. after incubation periods of 1-24 d, milk samples were assayed for beta-casomorphin-4, -5, -6 or -7 immunoreactive materials. in general, no beta-casomorphin immunoreactive material was found in samples incubated with non-caseolytic strains, e.g. pseudomonas putida o ...19853989066
naphthalene association and uptake in pseudomonas putida.two methods for bacterial membrane transport, filtration and flow dialysis, were used to study cellular association of pseudomonas putida with naphthalene. it is not technically possible to determine the exact cellular or vesicular location of the naphthalene, and because it is hydrophobic, it could be at the membrane(s) rather than inside the cells. as an index of naphthalene having crossed the inner membrane we used the intracellular formation of its first catabolite. an energized membrane or ...19863957866
a simple most probable number method for the enumeration of sulphate-reducing bacteria in biocide containing waters.a simple most probable number (mpn) method has been developed for the enumeration of sulphate-reducing bacteria (srb) in biocide-containing waters. the medium used is based on source water, it contains no toxic thioglycollate and is resistant to oxidation through mishandling. reduction is by a suspension of pseudomonas putida which acts as a powerful adsorbent of biguanide, phenolic, quaternary ammonium compound, glutaraldehyde and isothiazolone biocides. good recoveries of srb type strains were ...19853997694
crystal structure of muconate lactonizing enzyme at 6.5 a resolution.we have obtained crystals of pseudomonas putida muconate lactonizing enzyme. they diffract to better than 2.4 a resolution and have two monomers in the asymmetric unit, related by a non-crystallographic 2-fold axis. the cell dimensions are 139.3 a x 139.3 a x 84.1 a, and the space group is i4. the electron density map at 6.5 a resolution shows that the enzyme is an octamer with d4 symmetry.19853999146
tol plasmid pww15 contains two nonhomologous, independently regulated catechol 2,3-oxygenase genes.pseudomonas putida mt15 contains a 250-kilobase-pair (kbp) tol plasmid pww15, encoding toluene and xylene catabolism, which undergoes large spontaneous deletions to give two classes of mutants with altered catabolic phenotypes (h. keil and p. a. williams, j. gen. microbiol, 131:1023-1033, 1985). two structural genes for catechol 2,3-oxygenase (c23o) were cloned from pww15. the gene for c23oi was located on the 2.1-kbp xhoi fragment xh, whereas that for c23oii was found on the 11.5-kbp bamhi frag ...19854008443
the active transport of 2-keto-d-gluconate in vesicles prepared from pseudomonas purida.the transport of 2-keto-d-gluconate (alpha-d-arabino-2-hexulopyranosonic acid; 2kga) in vesicles prepared from glucose-grown pseudomonas putida occurs by a saturable process with a km of 110.0 +/- 2.9 microm and a vmax. of 0.55 +/- 0.04 nmol x min-1 x (mg of protein)-1. the provision of phenazine methosulphate/ascorbate or l-malate leads to an accumulation of intravescular 2kga, a decrease in the km value to 50 +/- 2.1 microm and 35 +/- 2.9 microm respectively and no change in the vmax. in the p ...19854004814
toxic effects of chlorinated and brominated alkanoic acids on pseudomonas putida pp3: selection at high frequencies of mutations in genes encoding dehalogenases.mutant strains of pseudomonas putida pp3 capable of utilizing monochloroacetate (mca) and dichloroacetate (dca) as the sole sources of carbon and energy were isolated from chemostat cultures. the mutants differed from the parent strain in that they could grow on products of mca and dca dehalogenation (catalyzed by inducible dehalogenases i and ii) and were resistant to growth inhibition by the two substrates. the growth inhibition of strain pp3 by mca, dca, and other halogenated alkanoic acids w ...19854015087
preliminary x-ray study of p-cresol methylhydroxylase (flavocytochrome c) from pseudomonas putida n.c.i.b. 9869.single crystals of p-cresol methylhydroxylase, a flavocytochrome c from pseudomonas putida, have been prepared. the crystals are orthorhombic, space group p212121 with unit cell parameters; a = 140.3 a, b = 130.6 a and c = 74.1 a. they contain a single non-symmetric dimer per asymmetric unit and diffract to at least 2.5 a resolution.19854020868
thermodynamic properties of oxidation-reduction reactions of bacterial, microsomal, and mitochondrial cytochromes p-450: an entropy-enthalpy compensation effect.an optically transparent thin-layer electrode cell with a very small volume was used for determination of the formal reduction potentials of bacterial, microsomal, and mitochondrial cytochromes p-450. at an extrapolated zero concentration of dye, the bacterial cytochrome from pseudomonas putida catalyzing the hydroxylation of camphor and the adrenal mitochondrial cytochrome catalyzing the cholesterol side-chain cleavage reaction had formal reduction potentials of -168 and -285 mv (ph 7.5 and 25 ...19863964682
the virulence of clinical and environmental isolates of campylobacter jejuni.the virulence of campylobacter jejuni and c. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to hela cells. variation in degree of attachment was observed, but this did not appear to be related to strain source, however, water strains were less invasive and less cytotoxic to hela cells than clinical strains as shown by immunofluorescence and electron microscopy. these differences were particularly evide ...19853973380
evidence against a temperature-dependent conformational change in urocanase from pseudomonas putida.enzymatic activity of urocanase (4-imidazolone-5-propionate hydro-lyase, ec 4.2.1.49) has an unusual resistance to temperature changes, and a temperature-dependent conformational change has been suggested (hug, d.h. and hunter, j.k. (1974) biochemistry 13, 1427-1431). a conformational change or dissociation has been proposed in the range of 29-31 degrees c (cohn, m.s., lynch, m.c. and phillips, a.t. (1975) biochim. biophys. acta 377, 444-453). in this work, no evidence was found for a temperatur ...19854016124
low resolution crystal structure of muconolactone isomerase. a decamer with a 5-fold symmetry axis.muconolactone isomerase from pseudomonas putida crystallizes from sodium sulfate solution in space group p2(1) (a = 65.84 a, b = 105.70 a, c = 77.20 a, beta = 90.5 degrees) with ten 11,000 mr subunits per asymmetric unit. the 7 a resolution crystal structure was solved by single isomorphous replacement followed by 10-fold symmetry averaging. the decameric enzyme has an uncommon non-crystallographic 5-fold symmetry axis and a large cavity in its center.19854032480
impact of cefotaxime on the fecal flora in children.a differential quantitative analysis was used to study the effect of cefotaxime on the fecal flora in 26 hospitalized children ranging from two days to four years of age. fecal specimens were obtained before, during and after therapy. this study was evaluated in comparison to 41 patients of the same age and from the same environment without antibiotic treatment or signs of infection. the fecal flora of the control group showed qualitative and quantitative stability. two groups of species were di ...19854055046
[stability of the npl-1 and npl-41 plasmids of naphthalene biodegradation in pseudomonas putida populations in continuous culture].the stability of biodegradation plasmids npl-1 and npl-41, which control the synthesis of enzymes for naphthalene oxidation to salicylate, was studied in pseudomonas putida bsa under the conditions of its continuous cultivation with limitation in glucose or salicylate in the chemostat regime and without limitation in the ph-stat regime. plasmid npl-1, which controls the inducible synthesis of naphthalene oxygenase, is stable in the population of p. putida cells under the conditions of continuous ...19854058326
[glucose consumption and dehydrogenase activity of the cells of the arsenite-oxidizing bacterium pseudomonas putida].the rates of glucose assimilation and dehydrogenase activity were studied in pseudomonas putida oxidizing arsenite. the rate of glucose utilization by the cells decreased in the presence of arsenites in the medium at the beginning because of the microbial adaptation to arsenite. the activity of dehydrogenase fell down when the cells were cultivated in the medium with arsenite. an inverse correlation existed between the rate of glucose assimilation and arsenite oxidation. apparently, arsenites we ...19854058329
formation and properties of flavoprotein-cytochrome hybrids by recombination of subunits from different species.p-cresol methylhydroxylases from four different pseudomonads differ in their isoelectric points and, to a lesser extent, in mr values and substrate specificity. the enzymes from three species were isolated in homogeneous form, then resolved into their flavoprotein and cytochrome subunits, and the subunits were recombined to yield the nine possible hybrids (i.e. three intraspecies and six interspecies). the resulting flavocytochromes showed extensive similarities in steady-state kinetic parameter ...19854062904
degradation of lawsone by pseudomonas putida l2.from humus obtained from stuttgart, a bacterium was isolated with lawsone (2-hydroxy-1,4-naphthoquinone) as selective source of carbon. this bacterium is capable of utilizing lawsone as sole source of carbon and energy. morphological and physiological characteristics of the bacterium were examined and it was identified as a strain of pseudomonas putida. the organism is referred to as pseudomonas putida l2. the degradation of lawsone by pseudomonas putida l2 was investigated. salicylic acid and c ...19854063065
development of broad-host-range vectors for expression of cloned genes in pseudomonas.the cloning and expression of genes in pseudomonas have been difficult, until now, due to the absence of vector systems that contain multiple restriction sites downstream from promoter sequences that are functional in pseudomonas. we report here the construction of several broad-host-range vectors that can be utilized in either pseudomonas or escherichia coli and that rely on easily selectable antibiotic resistance markers with multiple cloning sites. these vectors were constructed by inserting ...19854065575
scanning electron microscope study of pseudomonas putida colonies.pseudomonas putida colonies were examined by scanning electron microscope. a variety of cell morphologies, multicellular arrangements, and extracellular materials were observed in the fixed material. different regions of a single colony showed characteristic organizations of these architectural elements. in some cases, the detailed microstructure of the fixed colony surfaces observed by scanning electron microscopy could be correlated with macroscopic patterns visualized by histochemical stainin ...19854066611
the 2.6-a crystal structure of pseudomonas putida cytochrome p-450.the crystal structure of pseudomonas putida cytochrome p-450cam in the ferric, camphor bound form has been determined and partially refined to r = 0.23 at 2.6 a. the single 414 amino acid polypeptide chain (mr = 45,000) approximates a triangular prism with a maximum dimension of approximately 60 a and a minimum of approximately 30 a. twelve helical segments (a through l) account for approximately 40% of the structure while antiparallel beta pairs account for only approximately 10%. the unexposed ...19854066706
resolution of the flavocytochrome p-cresol methylhydroxylase into subunits and reconstitution of the enzyme.an improved procedure is described for the isolation of the flavocytochrome p-cresol methylhydroxylase (pcmh) from pseudomonas putida as well as methods for the separation of its subunits in native form and their recombination to reconstitute the original flavocytochrome. under appropriate conditions, the reconstitution is stoichiometric and results in complete recovery of the catalytic activity of the flavocytochrome. the separated flavoprotein subunit shows only 2% of the catalytic activity of ...19854074695
biodehalogenation: reactions of cytochrome p-450 with polyhalomethanes.the products, stoichiometry, and kinetics of the oxidation of the enzyme cytochrome p-450 cam by five polyhalomethanes and chloronitromethane are described. the reactivity of the enzyme is compared with that of deuteroheme and with the enzyme in its native cell, pseudomonas putida (ppg-786). in all cases, the reaction entails hydrogenolysis of the carbon-halogen bond: 2feiip + rcxn----2feiiip + rchxn-1 (p = porphyrin or p-450 cam in vitro and in vivo). trichloronitromethane was the fastest react ...19854039602
degradation of phenol by pseudomonas putida atcc 11172 in continuous culture at different ratios of biofilm surface to culture volume.pseudomonas putida atcc 11172 was grown in continuous culture with phenol as the only carbon and energy source; a culture practically without biofilm was compared with biofilm cultures of differing surface area/volume ratios. the biofilm did not significantly affect the maximal suspended cell concentration in the effluent, but it increased the maximal phenol reduction rate from 0.23 g/liter per h (without biofilm) to 0.72 g/liter per h at the highest biofilm level (5.5 cm2 of biofilm surface per ...19854083889
protein-protein interactions and antigenic relationships between the components of 4-methoxybenzoate monooxygenase and of benzene 1,2-dioxygenase from pseudomonas putida.the investigations presented in this paper were performed on two enzyme systems from pseudomonas putida: (a) 4-methoxybenzoate monooxygenase, consisting of a nadh: putidamonooxin oxidoreductase and putidamonooxin, the oxygen-activating component, and (b) benzene 1,2-dioxygenase, a three-component enzyme system with an nadh: ferredoxin oxidoreductase, functioning together with a plant-type ferredoxin as electron-transport chain, and an oxygen-activating component similar to putidamonooxin in its ...19854076185
versatile mercury-resistant cloning and expression vectors.cloning vectors have been constructed employing two diverse replicons, incq and p15a. both vectors confer resistance to kanamycin (km) and mercuric ions (hg2+). one of these vectors, pdg105, is a broad-host-range, nonconjugative, oligocopy incq plasmid, which is capable of transforming escherichia coli, acinetobacter calcoaceticus, and pseudomonas putida. the second vector, pdg106, is a narrow-host-range, multicopy cloning vector compatible with pbr322. both vectors contain unique cloning sites ...19854092936
regions of broad-host-range plasmid rk2 involved in replication and stable maintenance in nine species of gram-negative bacteria.the replication and maintenance properties of the broad-host-range plasmid rk2 and its derivatives were examined in nine gram-negative bacterial species. two regions of rk2, the origin of replication (oriv) and a segment that encodes for a replication protein (trfa delta kild, designated trfa*), are sufficient for replication in all nine species tested. however, stable maintenance of this minimal replicon (less than 0.3% loss per generation under nonselection conditions) is observed only in esch ...19854044529
mandelic acid-4-hydroxylase, a new inducible enzyme from pseudomonas convexa. 19734145649
purification and some properties of two isofunctional juglone hydroxylases from pseudomonas putida j1.juglone-induced cells of pseudomonas putida j 1 were shown to contain two isofunctional juglone hydroxylases. both enzymes were purified about 125-fold to homogeneity in polyacrylamide gel electrophoresis. the molecular masses of the native enzymes, as determined by sephacryl s-200 gel filtration were 59 000 da for enzyme 1 and 56 000 da for enzyme 2. the molecular masses of the subunits were determined by dodecyl sulfate polyacrylamide gel electrophoresis as 25 000 da (enzyme 1) and 23 500 da ( ...19854041238
pseudomonas putida cytochrome p-450: ligands of the substrate-free and substrate-bound states of the ferric hemoprotein. 19734149376
effect of temperature on urocanase from a psychrophile, pseudomonas putida. 19744150614
d- and l-isoleucine metabolism and regulation of their pathways in pseudomonas putida.pseudomonas putida oxidized isoleucine to acetyl-coenzyme a (coa) and propionyl-coa by a pathway which involved deamination of d-isoleucine by oxidation and l-isoleucine by transamination, oxidative decarboxylation, and beta oxidation at the ethyl side chain. at least three separate inductive events were required to form all of the enzymes of the pathway: d-amino acid dehydrogenase was induced during growth in the presence of d-isoleucine; branched-chain keto dehydrogenase was induced during gro ...19744150713
regulation of leucine catabolism in pseudomonas putida.the generation time of pseudomonas putida with l-leucine was 20 h in synthetic media but only 3 h with d-leucine. slow growth in the presence of l-leucine was partially overcome by addition of 0.1 mm amounts of either d-valine, l-valine, or 2-ketoisovalerate. the activities of five enzymes which take part in the oxidation of leucine by p. putida were measured under various conditions of growth. four enzymes were induced by growth with dl-leucine as sole source of carbon: d-amino acid dehydrogena ...19744150714
tyrosine motions in relation to the ferric spin equilibrium of cytochrome p-450cam.second derivative spectroscopy was used to determine the percentage of tyrosine residues that are exposed to solvent in cytochrome p-450cam isolated from pseudomonas putida. the ratio between two peak to trough second derivative absorbance differences has been shown to be dependent on the polarity of the microenvironment surrounding tyrosine residues [ragone, r., colonana, g., balestrieri, c., servillo, l., & irace, g. (1984) biochemistry 23, 1871]. with a number of camphor analogues that indepe ...19854084552
involvement of threonine dehydratase in biosynthesis of the alpha-ketobutyrate prosthetic group of urocanase.seventeen mutants of pseudomonas putida that were unable to grow on threonine as nitrogen source owing to a lack of threonine dehydratase were isolated, and all were found to be unable to synthesize active urocanase. spontaneous revertants selected for urocanase production concomitantly regained threonine dehydratase. mutants that were unable to utilize urocanate as carbon source were also isolated, and these were defective in urocanase formation but were normal in threonine dehydratase levels. ...19744154935
[catabolism of biphenyl by pseudomonas putida bs 893 strain containing the biodegradation plasmid pbs241].the isolation and identification of biphenyl catabolism products in pseudomonas putida bs 893 (pbs241) showed the presence of benzoic, m-hydroxybenzoic and cinnamic acids. the two latter compounds were not found in biphenyl degradation by other bacterial strains. p. putida bs 893 (pbs241) differed from other biphenyl-positive pseudomonas strains in the enzyme activity. these differences may stem from peculiarities in the pathway of biphenyl catabolism controlled by plasmid pbs241.19854094576
relationships among enzymes of the beta-ketoadipate pathway. ii. properties of crystalline beta-carboxy-cis,cis-muconate-lactonizing enzyme from pseudomonas putida. 19734199895
relationships among enzymes of the beta-ketoadipate pathway. 3. properties of crystalline gamma-carboxymuconolactone decarboxylase from pseudomonas putida. 19734199896
the degradation of l-histidine, imidazolyl-l-lactate and imidazolylpropionate by pseudomonas testosteroni.1. imidazol-5-ylpropionate and imidazol-5-yl-lactate are degraded by pseudomonas testosteroni via inducible pathways. 2. growth on either compound as the sole source of carbon results in the induction of the enzymes for histidine catabolism. 3. the pathway of histidine degradation in this organism, a non-fluorescent pseudomonad, is shown to be the same as that operating in pseudomonas fluorescens and pseudomonas putida. it consists of the successive formation of urocanate, imidazol-4-on-5-ylprop ...19734146796
relationships among enzymes of the beta-ketoadipate pathway. i. properties of cis,cis-muconate-lactonizing enzyme and muconolactone isomerase from pseudomonas putida. 19734199894
amino-terminal sequence of the tryptophan synthetase alpha chain of bacillus subtilis.the sequence of the 46 nh(2)-terminal residues of the tryptophan synthetase alpha chain of bacillus subtilis was determined and compared with the corresponding sequences of escherichia coli, shigella dysenteriae, salmonella typhimurium, aerobacter aerogenes, serratia marcescens, and pseudomonas putida. a deletion of six residues was found at the nh(2)-terminal end of the alpha chain of b. subtilis.19744206869
the metabolism of thymol by a pseudomonas.1. pseudomonas putida when grown with thymol contained a meta-fission dioxygenase, which required ferrous ions and readily cleaved the benzene nucleus of catechols between adjacent carbon atoms bearing hydroxyl and isopropyl groups. 2. 3-hydroxythymo-1,4-quinone was excreted towards the end of exponential growth and later was slowly metabolized. this compound was oxidized by partially purified extracts only when nadh was supplied; the substrate for the dioxygenase appeared to be 3-hydroxythymo-1 ...19684303067
formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by pseudomonas putida. 19704314232
effect of temperature on histidine ammonia-lyase from a psychrophile, pseudomonas putida.pseudomonas putida was able to grow at 0 c in a complex medium containing l-histidine and to synthesize histidine ammonia-lyase and urocanase. the activity of the former enzyme was assessed between -10 and 60 c in cells and in cell extracts. activity was maximal from 20 to 35 c. below 20 c, activity decreased with temperature but, significantly, the enzyme exhibited 30% of its maximal activity at 1.5 c. the temperature response was similar in both intact cells and cell extracts, which indicated ...19744152044
relationships among enzymes of the beta-ketoadipate pathway. iv. muconolactone isomerase from acinetobacter calcoaceticus and pseudomonas putida. 19744215816
methyrapone interaction with pseudomonas putida cytochrome p-405. 19714331031
isolation and characterization of a transducing phage for pseudomonas putida strain pmbl-1. 19714251736
the coexistence of two metabolic pathways in the meta cleavage of catechol by pseudomonas putida n.c.i.b. 10105. 19714333847
spin-state changes in cytochrome p-450cam on binding of specific substrates.the electron paramagnetic resonance signals of the soluble p-450 cytochrome from pseudomonas putida were observed at temperatures from 4.2 to 80 degrees k. as isolated, p-450 has a signal typical of a low spin ferric-heme compound with sulfur as one of the axial ligands (g = 2.45, 2.26, 1.91(5)). we also detected a minor signal typical of high spin ferric heme (g = 8, 4, 1.8) equivalent to less than 7% of the heme at temperatures below 20 degrees k. on titration with the substrate, (+)-camphor, ...19704319883
the iron electron-nuclear double resonance (endor) of two-iron ferredoxins from spinach, parsley, pig adrenal cortex and pseudomonas putida. 19714331268
metabolism of gallic acid and syringic acid by pseudomonas putida. 19724342601
the metabolism of benzoate and methylbenzoates via the meta-cleavage pathway by pseudomonas arvilla mt-2. 19724342906
the metabolic divergence in the meta cleavage of catechols by pseudomonas putida ncib 10015. physiological significance and evolutionary implications. 19724342908
a heat-stable nicotinamide-adenine dinucleotide glycohydrolase from pseudomonas putida kb1. partial purification and some properties of the enzyme and an inhibitory protein.a thermostable nad(p)(+) glycohydrolase (ec 3.2.2.6) detected in cell-free extracts of pseudomonas putida kb1 was purified to a single component on polyacrylamide-gel electrophoresis. a heat-labile inhibitor of the enzyme was also partially purified. enzyme free of inhibitor is present in culture supernatants. after an ultrasonic treatment enzyme-inhibitor complex and excess of inhibitor are present in both the cell-debris and soluble fractions. the general properties of the enzyme and inhibitor ...19724345853
the physiologic significance of the two divergent metabolic steps in the meta cleavage of catechols by pseudomonas putida n.c.i.b. 10105. 19714333848
the oxidation of nicotinic acid by pseudomonas ovalis chester. the terminal oxidase.in cell-free extracts of pseudomonas ovalis nicotinic acid oxidase is confined to the wallmembrane fraction. it is associated with an electron-transport chain comprising b- and c-type cytochromes only, differing proportions of which are reduced by nicotinate and nadh. co difference-spectra show two co-binding pigments, cytochrome o (absorption maximum at 417nm) and another component absorbing maximally at 425nm. cytochrome o is not reduced by nadh or by succinate but is by nicotinate, which can ...19724349118
pufification of a 4-methoxybenzoate o-demethylase from pseudomonas putida. 19714329101
pseudomonas putida cytochrome p-450: characterization of an oxygenated form of the hemoprotein. 19724335959
common enzymes of branched-chain amino acid catabolism in pseudomonas putida.two types of pseudomonas putida ppg2 mutants which were unable to degrade branched-chain amino acids were isolated after mutagenesis and selection for ability to grow on succinate, but not valine, as a sole source of carbon. these isolates were characterized by growth on the three branched-chain amino acids (valine, isoleucine, and leucine), on the corresponding branched-chain keto acids (2-ketoisovalerate, 2-keto-3-methylvalerate, and 2-ketoisocaproate), and on other selected intermediates as c ...19734352175
cytochrome c linked nicotinic acid hydroxylase in pseudomonas ovalis chester. 19734354821
the effects of trypsin on the membrane-bound nicotinic acid oxidase in pseudomonas ovalis chester. 19734359145
d-lysine catabolic pathway in pseudomonas putida: interrelations with l-lysine catabolism.the isolation of several mutant strains blocked in l-lysine degradation has permitted an assessment of the physiological significance of enzymatic reactions related to lysine metabolism in pseudomonas putida. additional studies with intact cells involved labeling of metabolic intermediates from radioactive l- or d-lysine, and patterns of enzyme induction in both wild-type and mutant strains. these studies lead to the conclusions that from l-lysine, the obligatory pathway is via delta-aminovalera ...19744359655
pseudomonas putida cytochrome p-450. binding of a spin-labeled analog of the inhibitor metyrapone. 19744364068
metabolism of phenol and cresols by mutants of pseudomonas putida.mutant strains of pseudomonas putida strain u have been obtained which are deficient in enzymes of the degradative pathways of phenol and cresols. mutant strains deficient in catechol 2, 3-oxygenase accumulated the appropriate catechol derivative from cresols. a mutant strain which would not grow on either phenol or a cresol was shown to be deficient in both 2-hydroxymuconic semialdehyde hydrolase and a nicotinamide adenine dinucleotide, oxidized form, (nad(+))-dependent aldehyde dehydrogenase. ...19734347965
purification and propeties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of pseudomonas putida.cells of pseudomonas putida, after growth with naphthalene as sole source of carbon and energy, contain an enzyme that oxidizes (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. the purified enzyme has a molecular weight of 102,000 and apparently consists of four 25,500 molecular weight subunits. the enzyme is specific for nicotinamide adenine dinucleotide as an electron acceptor and also oxidizes several other cis-dihydrodiols. however, no enzymatic activity was ob ...19744369091
crystallization and properties of p-hydroxybenzoate hydroxylase from pseudomonas putida. 19664380381
interactions of substrates with a purified 4-methoxybenzoate monooxygenase system (o-demethylating) from pseudomonas putida. 19734351526
a transmissible plasmid controlling camphor oxidation in pseudomonas putida.earlier papers demonstrated an extensive genetic exchange among fluorescent pseudomonads; this one documents for genes specifying enzymes of peripheral dissimilation an extrachromosomal array, segregation, and frequent interstrain transfer. an hypothesis is presented of a general mechanism for the formation and maintenance of metabolic diversity. the example used, the path of oxidative cleavage of the carbocyclic rings of the bicyclic monoterpene d- and l-camphor, terminates in acetate release a ...19734351810
studies on p-hydroxybenzoate hydroxylase from pseudomonas putida. 19694390689
[degradation of deoxysurgars by bacterial enzymes. v. purification and characterization of an nadp-dependent abequose dehydrogenase from pseudomonas putida]. 19684387016
a mutant of pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. 19744371622
[on the decomposition of deoxy sugars by vacterial enzymes, iv. comparative studies on the oxidation of 3-deoxy-d-galactose and d-galactose in a strain of pseudomonas putida]. 19684387175
urocanase of pseudomonas putida. subunit structure and origin of enzyme-bound -ketobutyrate. 19724404600
genetic control of the histidine dissimilatory pathway in pseudomonas putida. 19734405673
metabolism of benzoate and the methylbenzoates by pseudomonas putida (arvilla) mt-2: evidence for the existence of a tol plasmid.mutant strains of pseudomonas putida (arvilla) mt-2 which have lost the ability to grow at the expense of m- or p-toluate (methylbenzoate) but retain the ability to grow with benzoate arise spontaneously during growth on benzoate; this genetic loss occurs to a lesser extent during growth on nonaromatic carbon sources in the presence of mitomycin c. the mutants have totally lost the activity of the enzymes of the divergent meta pathway with the possible exception of 2-oxopent-4-enoate hydratase a ...19744418209
pseudomonas putida mutants defective in the metabolism of the products of meta fission of catechol and its methyl analogues.a selection procedure is described which was used to isolate mutants of pseudomonas putida strain u in the following enzymes of the meta-fission pathway of phenol and cresols: hydrolase, tautomerase, and decarboxylase. strains deficient in the hydrolase are unable to use either o- or m-cresol as a sole carbon source and were shown to accumulate 2-hydroxy-6-keto-2,4-heptadienoate when incubated in the presence of o- or m-cresol. when 2-hydroxymuconic semialdehyde (plus nicotinamide adenine dinucl ...19744418942
mechanism of action of p-hydroxybenzoate hydroxylase from pseudomonas putida. 3. the enzyme-substrate complex. 19714398470
bacterial degradation of 4-hydroxyphenylacetic acid and homoprotocatechuic acid.a species of acinetobacter and two strains of pseudomonas putida when grown with 4-hydroxyphenylacetic acid gave cell extracts that converted 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) into carbon dioxide, pyruvate, and succinate. the sequence of enzyme-catalyzed steps was as follows: ring-fission by a 2,3-dioxygenase, nicotinamide adenine dinucleotide-dependent dehydrogenation, decarboxylation, hydration, aldol fission, and oxidation of succinic semialdehyde. two new metabolites, ...19744420192
effect of carbamates and anilines on pseudomonas putida and soil microbiol activity. 19744418476
structural resemblance of cytochrome p-450 isolated from pseudomonas putida and from rabbit liver microsomes. 19744418751
continuous production of l-citrulline by immobilized pseudomonas putida cells. 19744441633
metabolism of biphenyl. structure and physicochemical properties of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, the meta-cleavage product from 2,3-dihydroxybiphenyl by pseudomonas putida.the structure of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid for the meta-cleavage product of 2,3-dihydroxybiphenyl by a pseudomonas putida strain was demonstrated on the basis of its chemical and physicochemical properties and those of its derivatives.19744462560
production of l-asparaginase by pseudomonas ovalis. 19744480497
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