Publications

acidobacteria strains from subdivision 1 act as plant growth-promoting bacteria.	acidobacteria is one of the most abundant phyla in soils and has been detected in rhizosphere mainly based on cultivation-independent approaches such as 16s rrna gene survey. although putative interaction of acidobacteria with plants was suggested, so far no plant-bacterial interactions were shown. therefore, we performed several in vitro tests to evaluate acidobacteria-plant interactions and the possible mechanisms involved in such interaction. we observed that arabidopsis thaliana inoculated w ...	2016	27339258
draft genome sequence of comamonas thiooxydans strain phe2-6 (nbrc 110656), a chlorinated-ethene-degrading bacterium.	comamonas thiooxydans strain phe2-6 (nbrc 110656), which was isolated from a trichloroethene-contaminated site in japan, utilizes phenol as a sole source of carbon and cometabolizes cis- and trans-dichloroethenes. we report here the draft genome sequence of this strain, containing 5,309,680 bp, with 60.6% g+c content.	2016	27340052
crystal structures of the global regulator dasr from streptomyces coelicolor: implications for the allosteric regulation of gntr/hutc repressors.	small molecule effectors regulate gene transcription in bacteria by altering the dna-binding affinities of specific repressor proteins. although the gntr proteins represent a large family of bacterial repressors, only little is known about the allosteric mechanism that enables their function. dasr from streptomyces coelicolor belongs to the gntr/hutc subfamily and specifically recognises operators termed dasr-responsive elements (dre-sites). its dna-binding properties are modulated by phosphoryl ...	2016	27337024
metabolomics analysis reveals the participation of efflux pumps and ornithine in the response of pseudomonas putida dot-t1e cells to challenge with propranolol.	efflux pumps are critically important membrane components that play a crucial role in strain tolerance in pseudomonas putida to antibiotics and aromatic hydrocarbons that result in these toxicants being expelled from the bacteria. here, the effect of propranolol on p. putida was examined by sudden addition of 0.2, 0.4 and 0.6 mg ml-1 of this β-blocker to several strains of p. putida, including the wild type dot-t1e and the efflux pump knockout mutants dot-t1e-ps28 and dot-t1e-18. bacterial viabi ...	2016	27331395
genomic potential of stenotrophomonas maltophilia in bioremediation with an assessment of its multifaceted role in our environment.	the gram negative bacterium stenotrophomonas is rapidly evolving as a nosocomial pathogen in immuno-compromised patients. treatment of stenotrophomonas maltophilia infections is problematic because of their increasing resistance to multiple antibiotics. this article aims to review the multi-disciplinary role of stenotrophomonas in our environment with special focus on their metabolic and genetic potential in relation to bioremediation and phytoremediation. current and emerging treatments and dia ...	2016	27446008
the induction and identification of novel colistin resistance mutations in acinetobacter baumannii and their implications.	acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. multidrug resistant a. baumannii has risen rapidly in vietnam, where colistin is becoming the drug of last resort for many infections. in this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible vietnamese isolates and one susceptible refere ...	2016	27329501
staphylococcus aureus coordinates leukocidin expression and pathogenesis by sensing metabolic fluxes via rpirc.	staphylococcus aureus is a formidable human pathogen that uses secreted cytolytic factors to injure immune cells and promote infection of its host. of these proteins, the bicomponent family of pore-forming leukocidins play critical roles in s. aureus pathogenesis. the regulatory mechanisms governing the expression of these toxins are incompletely defined. in this work, we performed a screen to identify transcriptional regulators involved in leukocidin expression in s. aureus strain usa300. we di ...	2016	27329753
metabolic analysis of the response of pseudomonas putida dot-t1e strains to toluene using fourier transform infrared spectroscopy and gas chromatography mass spectrometry.	an exceptionally interesting stress response of pseudomonas putida strains to toxic substances is the induction of efflux pumps that remove toxic chemical substances from the bacterial cell out to the external environment. to exploit these microorganisms to their full potential a deeper understanding of the interactions between the bacteria and organic solvents is required. thus, this study focuses on investigation of metabolic changes in p. putida upon exposure to toluene.	2016	27398079
clinical efficacy of polymyxin monotherapy versus nonvalidated polymyxin combination therapy versus validated polymyxin combination therapy in extensively drug-resistant gram-negative bacillus infections.	polymyxins have emerged as a last-resort treatment of extensively drug-resistant (xdr) gram-negative bacillus (gnb) infections, which present a growing threat. individualized polymyxin-based antibiotic combinations selected on the basis of the results of in vitro combination testing may be required to optimize therapy. a retrospective cohort study of hospitalized patients receiving polymyxins for xdr gnb infections from 2009 to 2014 was conducted to compare the treatment outcomes between patient ...	2016	27090177
genomics of high molecular weight plasmids isolated from an on-farm biopurification system.	the use of biopurification systems (bps) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. bps environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (mges), which play a key role in bacterial adaptation and evolution in such environments. here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-fa ...	2016	27321040
engineering of baeyer-villiger monooxygenase-based escherichia coli biocatalyst for large scale biotransformation of ricinoleic acid into (z)-11-(heptanoyloxy)undec-9-enoic acid.	baeyer-villiger monooxygenases (bvmos) are able to catalyze regiospecific baeyer-villiger oxygenation of a variety of cyclic and linear ketones to generate the corresponding lactones and esters, respectively. however, the enzymes are usually difficult to express in a functional form in microbial cells and are rather unstable under process conditions hindering their large-scale applications. thereby, we investigated engineering of the bvmo from pseudomonas putida kt2440 and the gene expression sy ...	2016	27311560
current technological improvements in enzymes toward their biotechnological applications.	enzymes from extremophiles are creating interest among researchers due to their unique properties and the enormous power of catalysis at extreme conditions. since community demands are getting more intensified, therefore, researchers are applying various approaches viz. metagenomics to increase the database of extremophilic species. furthermore, the innovations are being made in the naturally occurring enzymes utilizing various tools of recombinant dna technology and protein engineering, which a ...	2016	27379087
relationships between the regulatory systems of quorum sensing and multidrug resistance.	cell-cell communications, known as quorum sensing (qs) in bacteria, involve the signal molecules as chemical languages and the corresponding receptors as transcriptional regulators. in gram-negative bacteria, orphan luxr receptors recognize signals more than just acylhomoserine lactones, and modulate interspecies and interkingdom communications. whereas, in the gram-positive streptomyces, pseudo gamma-butyrolactones (gbls) receptors bind antibiotics other than gbl signals, and coordinate antibio ...	2016	27379084
poly-β-hydroxybutyrate metabolism is unrelated to the sporulation and parasporal crystal protein formation in bacillus thuringiensis.	poly-3-hydroxybutyrate (phb) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. it was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in bacillus thuringiensis. intriguingly, b. thuringiensis also accumulates large amounts of insecticidal crystal proteins (icps) during sporulation, which requires considerable input of carbon and energy sources. how phb accumulation affects sporulation and icp formation rem ...	2016	27379025
phosphorus mobilizing consortium mammoth p(™) enhances plant growth.	phosphorus (p) is a critical nutrient used to maximize plant growth and yield. current agriculture management practices commonly experience low plant p use efficiency due to natural chemical sorption and transformations when p fertilizer is applied to soils. a perplexing challenge facing agriculture production is finding sustainable solutions to deliver p more efficiently to plants. using prescribed applications of specific soil microbial assemblages to mobilize soil bound-p to improve crop nutr ...	2016	27326379
highly regio- and enantioselective multiple oxy- and amino-functionalizations of alkenes by modular cascade biocatalysis.	new types of asymmetric functionalizations of alkenes are highly desirable for chemical synthesis. here, we develop three novel types of regio- and enantioselective multiple oxy- and amino-functionalizations of terminal alkenes via cascade biocatalysis to produce chiral α-hydroxy acids, 1,2-amino alcohols and α-amino acids, respectively. basic enzyme modules 1-4 are developed to convert alkenes to (s)-1,2-diols, (s)-1,2-diols to (s)-α-hydroxyacids, (s)-1,2-diols to (s)-aminoalcohols and (s)-α-hy ...	2016	27297777
application of alkbgt and alkl from pseudomonas putida gpo1 for selective alkyl ester ω-oxyfunctionalization in escherichia coli.	the enzyme system alkbgt from pseudomonas putida gpo1 can efficiently ω-functionalize fatty acid methyl esters. outer membrane protein alkl boosts this ω-functionalization. in this report, it is shown that whole cells of escherichia coli expressing the alkbgt system can also ω-oxidize ethyl nonanoate (naee). coexpression of alkbgt and alkl resulted in 1.7-fold-higher ω-oxidation activity on naee. with this strain, initial activity on naee was 70 u/g (dry weight) of cells (gcdw), 67% of the initi ...	2016	27084021
a β-alanine catabolism pathway containing a highly promiscuous ω-transaminase in the 12-aminododecanate-degrading pseudomonas sp. strain aac.	we previously isolated the transaminase kes23458 from pseudomonas sp. strain aac as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. here, we report the subsequent characterization of this transaminase. it exhibits activity with a broad substrate range which includes α-, β-, and ω-amino acids, as well as α,ω-diamines and a number of other industrially relevant compounds. it is therefore a prospective candidate for the biosynthesis o ...	2016	27107110
identification of a third mn(ii) oxidase enzyme in pseudomonas putida gb-1.	the oxidation of soluble mn(ii) to insoluble mn(iv) is a widespread bacterial activity found in a diverse array of microbes. in the mn(ii)-oxidizing bacterium pseudomonas putida gb-1, two mn(ii) oxidase genes, named mnxg and mcoa, were previously identified; each encodes a multicopper oxidase (mco)-type enzyme. expression of these two genes is positively regulated by the response regulator mnxr. preliminary investigation into putative additional regulatory pathways suggested that the flagellar r ...	2016	27084014
symbiotic nitrogen fixation and the challenges to its extension to nonlegumes.	access to fixed or available forms of nitrogen limits the productivity of crop plants and thus food production. nitrogenous fertilizer production currently represents a significant expense for the efficient growth of various crops in the developed world. there are significant potential gains to be had from reducing dependence on nitrogenous fertilizers in agriculture in the developed world and in developing countries, and there is significant interest in research on biological nitrogen fixation ...	2016	27084023
proof of concept for the simplified breakdown of cellulose by combining pseudomonas putida strains with surface displayed thermophilic endocellulase, exocellulase and β-glucosidase.	the production and employment of cellulases still represents an economic bottleneck in the conversion of lignocellulosic biomass to biofuels and other biocommodities. this process could be simplified by displaying the necessary enzymes on a microbial cell surface. such an approach, however, requires an appropriate host organism which on the one hand can withstand the rough environment coming along with lignocellulose hydrolysis, and on the other hand does not consume the generated glucose so tha ...	2016	27287198
stenosis triggers spread of helical pseudomonas biofilms in cylindrical flow systems.	biofilms are multicellular bacterial structures that adhere to surfaces and often endow the bacterial population with tolerance to antibiotics and other environmental insults. biofilms frequently colonize the tubing of medical devices through mechanisms that are poorly understood. here we studied the helicoidal spread of pseudomonas putida biofilms through cylindrical conduits of varied diameters in slow laminar flow regimes. numerical simulations of such flows reveal vortical motion at stenoses ...	2016	27272623
the effect of using blood culture bottle of bronchoalveolar larvage fluid in pneumonia.	pneumonia is a primary cause of morbidity and mortality in infectious disease, and increasing antimicrobial resistance has raised concerns of treatment failure. therefore, we evaluated the value of a blood culture bottle for bronchoalveolar lavage (bal) samples on pathogen identification and on treatment modification in patients with pneumonia.	2016	27266871
chalcone-based selective inhibitors of a c4 plant key enzyme as novel potential herbicides.	weeds are a challenge for global food production due to their rapidly evolving resistance against herbicides. we have identified chalcones as selective inhibitors of phosphoenolpyruvate carboxylase (pepc), a key enzyme for carbon fixation and biomass increase in the c4 photosynthetic pathway of many of the world's most damaging weeds. in contrast, many of the most important crop plants use c3 photosynthesis. here, we show that 2',3',4',3,4-pentahydroxychalcone (ic50 = 600 nm) and 2',3',4'-trihyd ...	2016	27263468
characterization of genome-reduced bacillus subtilis strains and their application for the production of guanosine and thymidine.	genome streamlining has emerged as an effective strategy to boost the production efficiency of bio-based products. many efforts have been made to construct desirable chassis cells by reducing the genome size of microbes. it has been reported that the genome-reduced bacillus subtilis strain mbg874 showed clear advantages for the production of several heterologous enzymes including alkaline cellulase and protease. in addition to enzymes, b. subtilis is also used for the production of chemicals. to ...	2016	27260256
thigh abscess and necrotizing fasciitis following an inside-out transobturator tape intervention: a case report.	tension-free vaginal transobturator tapes are used worldwide in the treatment of urinary incontinence in women. very few severe complications have been described following this procedure, with no standard treatment yet established.	2016	27256334
muts regulates access of the error-prone dna polymerase pol iv to replication sites: a novel mechanism for maintaining replication fidelity.	translesion dna polymerases (pol) function in the bypass of template lesions to relieve stalled replication forks but also display potentially deleterious mutagenic phenotypes that contribute to antibiotic resistance in bacteria and lead to human disease. effective activity of these enzymes requires association with ring-shaped processivity factors, which dictate their access to sites of dna synthesis. here, we show for the first time that the mismatch repair protein muts plays a role in regulat ...	2016	27257069
biological control of rice bakanae by an endophytic bacillus oryzicola yc7007.	in our previous study, we reported that a novel endophytic bacterium bacillus oryzicola yc7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. this endophytic strain, b. oryzicola yc7007 was used as a biological control agent against bakanae disease of rice caused by fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. root drenching with b. oryzicola yc7007 suspension reduced the disease ...	2016	27298598
regulatory activities of four arsr proteins in agrobacterium tumefaciens 5a.	arsr is a well-studied transcriptional repressor that regulates microbe-arsenic interactions. most microorganisms have an arsr gene, but in cases where multiple copies exist, the respective roles or potential functional overlap have not been explored. we examined the repressors encoded by arsr1 and arsr2 (ars1 operon) and by arsr3 and arsr4 (ars2 operon) in agrobacterium tumefaciens 5a. arsr1 and arsr4 are very similar in their primary sequences and diverge phylogenetically from arsr2 and arsr3, ...	2016	27037117
highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element.	horizontal gene transfer is an important evolutionary mechanism for bacterial adaptation. however, given the typical low transfer frequencies in a bacterial population, little is known about the fate and interplay of donor cells and the mobilized dna during transfer. here we study transfer of an integrative and conjugative element (ice) among individual live bacterial cells. ices are widely distributed mobile dna elements that are different than plasmids because they reside silent in the host ch ...	2016	27247406
cultural, transcriptomic, and proteomic analyses of water-stressed cells of actinobacterial strains isolated from compost: ecological implications in the fed-batch composting process.	this study was undertaken to examine the effects of water activity (aw) on the viability of actinobacterial isolates from a fed-batch composting (fbc) process by comparing culturability and stainability with 5-cyano-2,3-ditoryl tetrazolium chloride (ctc). the fbc reactor as the source of these bacteria was operated with the daily loading of household biowaste for 70 d. during this period of composting, aw in the reactor decreased linearly with time and reached approximately 0.95 at the end of op ...	2016	27246805
high production of 3-hydroxypropionic acid in klebsiella pneumoniae by systematic optimization of glycerol metabolism.	3-hydroxypropionic acid (3-hp) is an important platform chemical proposed by the united states department of energy. 3-hp can be converted to a series of bulk chemicals. biological production of 3-hp has made great progress in recent years. however, low yield of 3-hp restricts its commercialization. in this study, systematic optimization was conducted towards high-yield production of 3-hp in klebsiella pneumoniae. we first investigated appropriate promoters for the key enzyme (aldehyde dehydroge ...	2016	27230116
rapid classification and identification of microcystis aeruginosa strains using maldi-tof ms and polygenetic analysis.	matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (maldi-tof ms) was used to establish a rapid, simple, and accurate method to differentiate among strains of microcystis aeruginosa, one of the most prevalent types of bloom-forming cyanobacteria. m. aeruginosa nies-843, for which a complete genome has been sequenced, was used to characterize ribosomal proteins as biomarkers and to optimize conditions for observing ribosomal proteins as major peaks in a given mass spectr ...	2016	27227555
when genome-based approach meets the "old but good": revealing genes involved in the antibacterial activity of pseudomonas sp. p482 against soft rot pathogens.	dickeya solani and pectobacterium carotovorum subsp. brasiliense are recently established species of bacterial plant pathogens causing black leg and soft rot of many vegetables and ornamental plants. pseudomonas sp. strain p482 inhibits the growth of these pathogens, a desired trait considering the limited measures to combat these diseases. in this study, we determined the genetic background of the antibacterial activity of p482, and established the phylogenetic position of this strain. pseudomo ...	2016	27303376
crystal structure of cytochrome p450 (cyp105p2) from streptomyces peucetius and its conformational changes in response to substrate binding.	cytochrome p450 monooxygenases (cyp, ec 1.14.14.1) belong to a large family of enzymes that catalyze the hydroxylation of various substrates. here, we present the crystal structure of cyp105p2 isolated from streptomyces peucetius atcc27952 at a 2.1 å resolution. the structure shows the presence of a pseudo-ligand molecule in the active site, which was co-purified fortuitously and is presumed to be a biphenyl derivative. comparison with previously determined substrate-bound cyp structures showed ...	2016	27231902
polyhydroxyalkanoate (pha) granules have no phospholipids.	polyhydroxybutyrate (phb) granules, also designated as carbonosomes, are supra-molecular complexes in prokaryotes consisting of a phb polymer core and a surface layer of structural and functional proteins. the presence of suspected phospholipids in the surface layer is based on in vitro data of isolated phb granules and is often shown in cartoons of the phb granule structure in reviews on phb metabolism. however, the in vivo presence of a phospholipid layer has never been demonstrated. we addres ...	2016	27222167
potentiation of aminoglycoside activity in pseudomonas aeruginosa by targeting the amgrs envelope stress-responsive two-component system.	a screen for agents that potentiated the activity of paromomycin (par), a 4,5-linked aminoglycoside (ag), against wild-type pseudomonas aeruginosa identified the rna polymerase inhibitor rifampin (rif). rif potentiated additional 4,5-linked ags, such as neomycin and ribostamycin, but not the clinically important 4,6-linked ags amikacin and gentamicin. potentiation was absent in a mutant lacking the amgrs envelope stress response two-component system (tcs), which protects the organism from ag-gen ...	2016	27021319
identification, characterization and molecular adaptation of class i redox systems for the production of hydroxylated diterpenoids.	de novo production of multi-hydroxylated diterpenoids is challenging due to the lack of efficient redox systems.	2016	27216162
interrupting peptidoglycan deacetylation during bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts.	the peptidoglycan wall, located in the periplasm between the inner and outer membranes of the cell envelope in gram-negative bacteria, maintains cell shape and endows osmotic robustness. predatory bdellovibrio bacteria invade the periplasm of other bacterial prey cells, usually crossing the peptidoglycan layer, forming transient structures called bdelloplasts within which the predators replicate. prey peptidoglycan remains intact for several hours, but is modified and then degraded by escaping p ...	2016	27211869
a wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds.	the main purpose of this study was to assess the actual occurrence of gram-negative oxidase-positive bacteria (gnop) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. we report a prospective series of 92 wound samples. routine culturing was combined with a procedure optimised for fastidious gnop. all gnop isolates were identified by 16s rdna sequencing to the species level. we observed a more prominent role of gnop, including at least 30 spec ...	2016	27197725
industrial production of acetone and butanol by fermentation-100 years later.	microbial production of acetone and butanol was one of the first large-scale industrial fermentation processes of global importance. during the first part of the 20th century, it was indeed the second largest fermentation process, superseded in importance only by the ethanol fermentation. after a rapid decline after the 1950s, acetone-butanol-ethanol (abe) fermentation has recently gained renewed interest in the context of biorefinery approaches for the production of fuels and chemicals from ren ...	2016	27199350
the regulation of para-nitrophenol degradation in pseudomonas putida dll-e4.	pseudomonas putida dll-e4 can efficiently degrade para-nitrophenol and its intermediate metabolite hydroquinone. the regulation of para-nitrophenol degradation was studied, and pnp induced a global change in the transcriptome of p. putida dll-e4. when grown on pnp, the wild-type strain exhibited significant downregulation of 2912 genes and upregulation of 845 genes, whereas 2927 genes were downregulated and 891 genes upregulated in a pnpr-deleted strain. genes related to two non-coding rnas (ins ...	2016	27191401
evaluation of heavy metal removal from wastewater in a modified packed bed biofilm reactor.	for the effective application of a modified packed bed biofilm reactor (pbbr) in wastewater industrial practice, it is essential to distinguish the tolerance of the system for heavy metals removal. the industrial contamination of wastewater from various sources (e.g. zn, cu, cd and ni) was studied to assess the impacts on a pbbr. this biological system was examined by evaluating the tolerance of different strengths of composite heavy metals at the optimum hydraulic retention time (hrt) of 2 hour ...	2016	27186636
global changes in the proteome of cupriavidus necator h16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates.	synthesis of poly-[3-hydroxybutyrate] (phb) by cupriavidus necator h16 in batch cultures was evaluated using three biodiesel-derived by-products as the sole carbon sources: waste glycerol (reg-80, refined to 80 % purity with negligible free fatty acids); glycerol bottom (reg-gb, with up to 65 % glycerol and 35 % free fatty acids), and free fatty acids (reg-ffa, with up to 75 % ffa and no glycerol). all the three substrates supported growth and phb production by c. necator, with polymer accumulat ...	2016	27184362
effects of polycyclic aromatic hydrocarbon mixtures on degradation, gene expression, and metabolite production in four mycobacterium species.	polycyclic aromatic hydrocarbons (pahs) are widespread environmental contaminants that are hazardous to human health. it has been demonstrated that members of the mycobacterium genus are among the most effective degraders of pahs, but few studies have focused on the degradation of pah mixtures. in this study, single and mixed pah metabolism was investigated in four phylogenetically distinct mycobacterium species with respect to (i) parent compound degradation, (ii) bacterial growth, (iii) catabo ...	2016	27037123
a new mercury-accumulating mucor hiemalis strain eh8 from cold sulfidic spring water biofilms.	here, we report about a unique aquatic fungus mucor hiemaliseh8 that can remove toxic ionic mercury from water by intracellular accumulation and reduction into elemental mercury (hg(0) ). eh8 was isolated from a microbial biofilm grown in sulfidic-reducing spring water sourced at a marching's site located downhill from hop cultivation areas with a history of mercury use. a thorough biodiversity survey and mercury-removal function analyses were undertaken in an area of about 200 km(2) in bavaria ...	2016	27177603
different inocula produce distinctive microbial consortia with similar lignocellulose degradation capacity.	despite multiple research efforts, the current strategies for exploitation of lignocellulosic plant matter are still far from optimal, being hampered mostly by the difficulty of degrading the recalcitrant parts. an interesting approach is to use lignocellulose-degrading microbial communities by using different environmental sources of microbial inocula. however, it remains unclear whether the inoculum source matters for the degradation process. here, we addressed this question by verifying the l ...	2016	27170322
the preprotein translocase yidc controls respiratory metabolism in mycobacterium tuberculosis.	the yidc-oxa1-alb3 preprotein translocases play a vital role in membrane insertion of proteins in eukaryotes and bacteria. in a recent study we observed that rv3921c, which encodes putative yidc translocase in mycobacterium tuberculosis (mtb), is essential for in vitro growth of bacteria. however, the exact function of this particular protein remains to identify in mycobacterial pathogens. by performing a systematic study here we show that yidc of mtb is an envelope protein, which is required fo ...	2016	27166092
editorial: wired for life.		2016	27242692
integrated analysis of gene expression and metabolic fluxes in pha-producing pseudomonas putida grown on glycerol.	given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. the soil bacterium pseudomonas putida kt2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-pha), a class of biopolymers of industrial interest. here, glycerol metabolism in p. putida kt2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, gro ...	2016	27142075
mycelium-like networks increase bacterial dispersal, growth, and biodegradation in a model ecosystem at various water potentials.	fungal mycelia serve as effective dispersal networks for bacteria in water-unsaturated environments, thereby allowing bacteria to maintain important functions, such as biodegradation. however, poor knowledge exists on the effects of dispersal networks at various osmotic (ψo) and matric (ψm) potentials, which contribute to the water potential mainly in terrestrial soil environments. here we studied the effects of artificial mycelium-like dispersal networks on bacterial dispersal dynamics and subs ...	2016	26944849
in vitro and in vivo antimicrobial activity of combined therapy of silver nanoparticles and visible blue light against pseudomonas aeruginosa.	silver nanoparticles (agnps) have been used as potential antimicrobial agents against resistant pathogens. we investigated the possible therapeutic use of agnps in combination with visible blue light against a multidrug resistant clinical isolate of pseudomonas aeruginosa in vitro and in vivo. the antibacterial activity of agnps against p. aeruginosa (1×10(5) colony forming unit/ml) was investigated at its minimal inhibitory concentration (mic) and sub-mic, alone and in combination with blue lig ...	2016	27175075
effects of bloom-forming cyanobacterial extracellular polymeric substances on the adsorption of cadmium onto kaolinite: behaviors and possible mechanisms.	cyanobacterial blooms result in high level of cyanobacterial extracellular polymeric substances (eps) in water. the effects of bloom-forming cyanobacterial eps on the distribution of cd(ii) in the interface between sediment and water is unknown. clay is a main component in sediment. the effects of eps, originated from a typical bloom-forming cyanobacterium microcystis aeruginosa, on the adsorption and desorption characteristics of cd(ii) by kaolinite were investigated in this study. results of x ...	2016	27186506
metabolic fingerprinting of pseudomonas putida dot-t1e strains: understanding the influence of divalent cations in adaptation mechanisms following exposure to toluene.	pseudomonas putida strains can adapt and overcome the activity of toxic organic solvents by the employment of several resistant mechanisms including efflux pumps and modification to lipopolysaccharides (lps) in their membranes. divalent cations such as magnesium and calcium play a crucial role in the development of solvent tolerance in bacterial cells. here, we have used fourier transform infrared (ft-ir) spectroscopy directly on cells (metabolic fingerprinting) to monitor bacterial response to ...	2016	27128955
bioprospecting of plant growth promoting bacilli and related genera prevalent in soils of pristine sacred groves: biochemical and molecular approach.	bacillus spp. and related genera native to soils of the pristine sacred groves from meghalaya, india were characterized using biochemical and 16s rrna gene analysis which revealed dominance of bacillus, paenibacillus, lysinibacillus and viridibacillus in the groves. biochemical estimation was carried out for in vitro testing of plant growth promoting traits present in these isolates. pcr screening were performed for plant growth-promoting related genes involved in the biosynthesis of acid phosph ...	2016	27111883
degradation of polynuclear aromatic hydrocarbons by two strains of pseudomonas.	the goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at mcdoel switchyard in bloomington, indiana. using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains pb1 and pb2. applying the ribotyping technique using the 16s rrna gene analysis, the strains were assigned to the genus pseudomonas (pseudomonas plecogl ...	2016	27245129
evaluation of bacterial diversity recovered from petroleum samples using different physical matrices.	unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. despite the techniques developed in order to improve methodologies involving dna extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of dna from environments with low cellular density for further microbiological analyses. this work aimed at the evaluation of different matrices (arenite ...	2016	27282730
the bark-beetle-associated fungus, endoconidiophora polonica, utilizes the phenolic defense compounds of its host as a carbon source.	norway spruce (picea abies) is periodically attacked by the bark beetle ips typographus and its fungal associate, endoconidiophora polonica, whose infection is thought to be required for successful beetle attack. norway spruce produces terpenoid resins and phenolics in response to fungal and bark beetle invasion. however, how the fungal associate copes with these chemical defenses is still unclear. in this study, we investigated changes in the phenolic content of norway spruce bark upon e. polon ...	2016	27208235
a phopq-regulated abc transporter system exports tetracycline in pseudomonas aeruginosa.	pseudomonas aeruginosa is an important human pathogen whose infections are difficult to treat due to its high intrinsic resistance to many antibiotics. here, we show that the disruption of pa4456, encoding the atp binding component of a putative atp-binding cassette (abc) transporter, increased the bacterium's susceptible to tetracycline and other antibiotics or toxic chemicals. fluorescence spectroscopy and antibiotic accumulation tests showed that the interruption of the abc transporter caused ...	2016	26953208
waste workers' exposure to airborne fungal and bacterial species in the truck cab and during waste collection.	a large number of people work with garbage collection, and exposure to microorganisms is considered an occupational health problem. however, knowledge on microbial exposure at species level is limited. the aim of the study was to achieve knowledge on waste collectors' exposure to airborne inhalable fungal and bacterial species during waste collection with focus on the transport of airborne microorganisms into the truck cab. airborne microorganisms were collected with samplers mounted in the truc ...	2016	27098185
an enantio- and diastereoselective chemoenzymatic synthesis of α-fluoro β-hydroxy carboxylic esters.	the trans-o-hydroxybenzylidene pyruvate aldolase-catalysed reactions between fluoropyruvate and many (hetero)aromatic aldehydes yield aldol adducts without subsequent dehydration. treatment of the reaction products with hydrogen peroxide yields the corresponding syn-configured α-fluoro β-hydroxy carboxylic acids which have >98 % ee. the overall chemoenzymatic approach, in which fluoropyruvate serves as a fluoroacetate equivalent, may be exploited in the synthesis of polar building blocks and fra ...	2016	27090612
impact of the chromatin remodeling factor chd1 on gut microbiome composition of drosophila melanogaster.	the composition of the intestinal microbiota of drosophila has been studied in some detail in recent years. environmental, developmental and host-specific genetic factors influence microbiome composition in the fly. our previous work has indicated that intestinal bacterial load can be affected by chromatin-targeted regulatory mechanisms. here we studied a potential role of the conserved chromatin assembly and remodeling factor chd1 in the shaping of the gut microbiome in drosophila melanogaster. ...	2016	27093431
phenylacetic acid is isr determinant produced by bacillus fortis iags162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt.	bacillus fortis iags162 has been previously shown to induce systemic resistance in tomato plants against fusarium wilt disease. in the first phase of current study, the isr determinant was isolated from extracellular metabolites of this bacterium. isr bioassays combined with solvent extraction, column chromatography and gc/ms analysis proved that phenylacetic acid (paa) was the potential isr determinant that significantly ameliorated fusarium wilt disease of tomato at concentrations of 0.1 and 1 ...	2016	27148321
mechanisms of bacterial (serratia marcescens) attachment to, migration along, and killing of fungal hyphae.	we have found a remarkable capacity for the ubiquitous gram-negative rod bacterium serratia marcescens to migrate along and kill the mycelia of zygomycete molds. this migration was restricted to zygomycete molds and several basidiomycete species. no migration was seen on any molds of the phylum ascomycota. s. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well.s. marcescens did not exhibit growth tropism toward zygo ...	2016	26896140
engineering a predatory bacterium as a proficient killer agent for intracellular bio-products recovery: the case of the polyhydroxyalkanoates.	this work examines the potential of the predatory bacterium bdellovibrio bacteriovorus hd100, an obligate predator of other gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. the bio-product targets to be recovered were polyhydroxyalkanoates (phas) produced naturally by pseudomonas putida and cupriavidus necator, or by recombinant escherichia coli strains. b. bacteriovorus with a mutated pha depolymerase gene to p ...	2016	27087466
genome-wide analysis and expression profiling of glyoxalase gene families in soybean (glycine max) indicate their development and abiotic stress specific response.	glyoxalase pathway consists of two enzymes, glyoxalase i (glyi) and glyoxalase ii (glyii) which detoxifies a highly cytotoxic metabolite methylglyoxal (mg) to its non-toxic form. mg may form advanced glycation end products with various cellular macro-molecules such as proteins, dna and rna; that ultimately lead to their inactivation. role of glyoxalase enzymes has been extensively investigated in various plant species which showed their crucial role in salinity, drought and heavy metal stress to ...	2016	27083416
draft genome sequence of pseudomonas putida bw11m1, a banana rhizosphere isolate with a diversified antimicrobial armamentarium.	in this study, we report the draft genome ofpseudomonas putidabw11m1, a banana rhizosphere isolate producing various antimicrobial compounds, including a lectin-like bacteriocin, an r-type tailocin, the cyclic lipopeptide xantholysin, and the fatty acid-derived pseudopyronine.	2016	27081131
cd-resistant strains of b. cereus s5 with endurance capacity and their capacities for cadmium removal from cadmium-polluted water.	the goal of this study was to identify cd-resistant bacterial strains with endurance capacity and to evaluate their ability to remove cadmium ions from cadmium-polluted water. the bacillus cereuss5 strain identified in this study had the closest genetic relationship with b. cereus sp. cp1 and performed well in the removal of cd2+ions from solution. the results showed that both the live and dead biomasses of the cd2+-tolerant b. cereus s5 strain could absorb cd2+ ions in solution but that the liv ...	2016	27077388
reduced expression of cytochrome oxidases largely explains camp inhibition of aerobic growth in shewanella oneidensis.	inhibition of bacterial growth under aerobic conditions by elevated levels of cyclic adenosine 3',5'-monophosphate (camp), first revealed more than 50 years ago, was attributed to accumulation of toxic methylglyoxal (mg). here, we report a crp-dependent mechanism rather than mg accumulation that accounts for the phenotype in shewanella oneidensis, an emerging research model for the bacterial physiology. we show that a similar phenotype can be obtained by removing cpda, a camp phosphodiesterase t ...	2016	27076065
comprehensive molecular characterization of methylobacterium extorquens am1 adapted for 1-butanol tolerance.	the toxicity of alcohols is one of the major roadblocks of biological fermentation for biofuels production. methylobacterium extorquens am1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from cheap carbon feedstocks through a synthetic metabolic pathway. however, m. extorquens am1 is vulnerable to solvent stress, which impedes further development for 1-butanol production. only a few studies have reported the general stress response of m. extorquens am1 ...	2016	27069508
exploring the modular nature of riboswitches and rna thermometers.	natural regulatory rnas like riboswitches and rna thermometers (rnat) have considerable potential in synthetic biology. they are located in the 5' untranslated region (utr) of bacterial mrnas and sense small molecules or changes in temperature, respectively. while riboswitches act on the level of transcription, translation or mrna stability, all currently known rnats regulate translation initiation. in this study, we explored the modularity of riboswitches and rnats and obtained regulatory devic ...	2016	27060146
draft genome sequences of three bacillus species from south african marine sponges.	the rise in antibiotic-resistant bacteria has spurred efforts to identify novel compounds with antimicrobial activity. this brief report describes the genome sequence of threebacillusspecies isolates from south african marine sponges, which produce compounds with antimicrobial activity. a search for secondary metabolite clusters revealed several secondary metabolite pathways in these genomes, which may hold promise as novel antibiotics.	2016	27056214
a multi-enzymatic cascade reaction for the stereoselective production of γ-oxyfunctionalyzed amino acids.	a stereoselective three-enzyme cascade for synthesis of diasteromerically pure γ-oxyfunctionalized α-amino acids was developed. by coupling a dynamic kinetic resolution (dkr) using an n-acylamino acid racemase (naaar) and an l-selective aminoacylase from geobacillus thermoglucosidasius with a stereoselective isoleucine dioxygenase from bacillus thuringiensis, diastereomerically pure oxidized amino acids were produced from racemic n-acetylamino acids. the three enzymes differed in their optimal t ...	2016	27092111
rna-seq transcriptome analysis of a pseudomonas strain with diversified catalytic properties growth under different culture medium.	biocatalysis is an emerging strategy for the production of enantio-pure organic molecules. however, lacking of commercially available enzymes restricts the widespread application of biocatalysis. in this study, we report a pseudomonas strain which exhibited versatile oxidation activity to synthesize chiral sulfoxides when growing under m9-toluene medium and reduction activity to synthesize chiral alcohols when on luria-bertani (lb) medium, respectively. further comparative transcriptome analysis ...	2016	27061463
modulating the import of medium-chain alkanes in e. coli through tuned expression of fadl.	in recent years, there have been intensive efforts to develop synthetic microbial platforms for the production, biosensing and bio-remediation of fossil fuel constituents such as alkanes. building predictable engineered systems for these applications will require the ability to tightly control and modulate the rate of import of alkanes into the host cell. the native components responsible for the import of alkanes within these systems have yet to be elucidated. to shed further insights on this, ...	2016	27053948
convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria.	low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. the efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use.	2016	27044351
singly flagellated pseudomonas aeruginosa chemotaxes efficiently by unbiased motor regulation.	pseudomonas aeruginosais an opportunistic human pathogen that has long been known to chemotax. more recently, it has been established that chemotaxis is an important factor in the ability ofp. aeruginosato make biofilms. genes that allowp. aeruginosato chemotax are homologous with genes in the paradigmatic model organism for chemotaxis,escherichia coli however,p. aeruginosais singly flagellated ande. colihas multiple flagella. therefore, the regulation of counterclockwise/clockwise flagellar mot ...	2016	27048795
clinical usefulness of arbekacin.	arbekacin is a broad-spectrum aminoglycoside used to treat methicillin-resistant staphylococcus aureus (mrsa). arbekacin has antibacterial activities against high-level gentamicin-resistant enterococci, multidrug-resistant pseudomonas aeruginosa, and acinetobacter baumannii et al. here, we reviewed in vitro data on arbekacin in staphylococci and gram-negative microorganisms. we also reviewed clinical studies for clinical efficacy and microbiologic efficacy data in patients with identified mrsa a ...	2016	27104010
structural elucidation and toxicity assessment of degraded products of aflatoxin b1 and b2 by aqueous extracts of trachyspermum ammi.	in this study aqueous extract of seeds and leaves of trachyspermum ammi were evaluated for their ability to detoxify aflatoxin b1 and b2 (afb1; 100 μg l(-1) and afb2; 50 μg l(-1)) by in vitro and in vivo assays. results indicated that t. ammi seeds extract was found to be significant (p < 0.05) in degrading afb1 and afb2 i.e., 92.8 and 91.9% respectively. however, t. ammi leaves extract proved to be less efficient in degrading these aflatoxins, under optimized conditions i.e., ph 8, temperature ...	2016	27064492
the frurba operon is necessary for group a streptococcal growth in fructose and for resistance to neutrophil killing during growth in whole human blood.	bacterial pathogens rely on the availability of nutrients for survival in the host environment. the phosphoenolpyruvate-phosphotransferase system (pts) is a global regulatory network connecting sugar uptake with signal transduction. since the fructose pts has been shown to impact virulence in several streptococci, including the human pathogen streptococcus pyogenes(the group a streptococcus[gas]), we characterized its role in carbon metabolism and pathogenesis in the m1t1 strain 5448. growth in ...	2016	26787724
insights into the function of a second, nonclassical ahp peroxidase, ahpa, in oxidative stress resistance in bacillus subtilis.	organisms growing aerobically generate reactive oxygen-containing molecules, such as hydrogen peroxide (h2o2). these reactive oxygen molecules damage enzymes and dna and may even cause cell death. in response, bacillus subtilis produces at least nine potential peroxide-scavenging enzymes, two of which appear to be the primary enzymes responsible for detoxifying peroxides during vegetative growth: a catalase (encoded by kata) and an alkylhydroperoxide reductase (ahp, encoded by ahpc). ahpc uses t ...	2016	26787766
transcriptional profiling of klebsiella pneumoniae defines signatures for planktonic, sessile and biofilm-dispersed cells.	surface-associated communities of bacteria, known as biofilms, play a critical role in the persistence and dissemination of bacteria in various environments. biofilm development is a sequential dynamic process from an initial bacterial adhesion to a three-dimensional structure formation, and a subsequent bacterial dispersion. transitions between these different modes of growth are governed by complex and partially known molecular pathways.	2016	26979871
linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in thauera linaloolentis 47lol.	thauera linaloolentis 47lol uses the tertiary monoterpene alcohol (r,s)-linalool as sole carbon and energy source under denitrifying conditions. the conversion of linalool to geraniol had been observed in carbon-excess cultures, suggesting the presence of a 3,1-hydroxyl-δ(1)-δ(2)-mutase (linalool isomerase) as responsible enzyme. to date, only a single enzyme catalyzing such a reaction is described: the linalool dehydratase/isomerase (ldi) from castellaniella defragrans 65phen acting only on (s) ...	2016	26979141
potential biotechnological strategies for the cleanup of heavy metals and metalloids.	global mechanization, urbanization, and various natural processes have led to the increased release of toxic compounds into the biosphere. these hazardous toxic pollutants include a variety of organic and inorganic compounds, which pose a serious threat to the ecosystem. the contamination of soil and water are the major environmental concerns in the present scenario. this leads to a greater need for remediation of contaminated soils and water with suitable approaches and mechanisms. the conventi ...	2016	27014323
whole-genome identification, phylogeny, and evolution of the cytochrome p450 family 2 (cyp2) subfamilies in birds.	the cytochrome p450 (cyp) superfamily defends organisms from endogenous and noxious environmental compounds, and thus is crucial for survival. however, beyond mammals the molecular evolution of cyp2 subfamilies is poorly understood. here, we characterized the cyp2 family across 48 avian whole genomes representing all major extant bird clades. overall, 12 cyp2 subfamilies were identified, including the first description of the cyp2f, cyp2g, and several cyp2af genes in avian genomes. some of the c ...	2016	26979796
matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of penicillium marneffei.	penicillium marneffei is the most important thermal dimorphic fungus causing systemic mycosis in hiv-infected and other immunocompromised patients in southeast asia. however, laboratory diagnosis of penicilliosis, which relies on microscopic morphology and mycelial-to-yeast conversion, is time-consuming and expertise-dependent, thus delaying diagnosis and treatment. although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is useful for identification ...	2016	26965891
genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant st235 isolates of pseudomonas aeruginosa.	pseudomonas aeruginosa are noscomially acquired, opportunistic pathogens that pose a major threat to the health of burns patients and the immunocompromised. we sequenced the genomes of p. aeruginosa isolates rns_pa1, rns_pa46 and rns_pae05, which displayed resistance to almost all frontline antibiotics, including gentamicin, piperacillin, timentin, meropenem, ceftazidime and colistin. we provide evidence that the isolates are representatives of p. aeruginosa sequence type (st) 235 and carry tn61 ...	2016	26962050
biodegradation of phenol by bacteria strain acinetobacter calcoaceticus pa isolated from phenolic wastewater.	a phenol-degrading bacterium strain pa was successfully isolated from the effluent of petrochemical wastewater. based on its morphological, physiological and biochemical characteristics, the strain pa was characterized as a gram-negative, strictly aerobic, nonmotile and short rod-shaped bacterium that utilizes phenol as a sole carbon and energy source. 16s rdna sequence analysis revealed that this strain is affiliated to acinetobacter calcoaceticus in the group of gammaproteobacteria. the strain ...	2016	27005648
using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective michaelases.	the michael-type addition reaction is widely used in organic synthesis for carbon-carbon bond formation. however, biocatalytic methodologies for this type of reaction are scarce, which is related to the fact that enzymes naturally catalysing carbon-carbon bond-forming michael-type additions are rare. a promising template to develop new biocatalysts for carbon-carbon bond formation is the enzyme 4-oxalocrotonate tautomerase, which exhibits promiscuous michael-type addition activity. here we prese ...	2016	26952338
dynamic clustering of the bacterial sensory kinase baes.	several bacterial sensory-kinase receptors form clusters on the cell membrane. however, the dynamics of sensory-kinase clustering are largely unclear. using measurements of fluorescence anisotropy and time-lapse imaging of escherichia coli cells, we demonstrate that copper ions trigger self-association of baes receptors and lead to rapid formation of clusters, which can be reversibly dispersed by a metal chelator. copper ions did not trigger self-association of other fluorescently tagged sensory ...	2016	26950881
two superoxide dismutases from tnotchr are involved in detoxification of reactive oxygen species induced by chromate.	superoxide dismutases (sod) have been reported as the most relevant bacterial enzymes involved in cells protection from reactive oxygen species (ros). these toxic species are often the product of heavy metal stress.	2016	26944876
draft genome sequence of a chlorinated-ethene degrader, cupriavidus necator strain phe3-6 (nbrc 110655).	cupriavidus necator strain phe3-6 grows on phenol as a sole carbon source and cometabolizes cis- and trans-dichloroethenes and trichloroethene. here, we report the draft genome sequence of phe3-6, which provides insights into the degradation system of phenol and chlorinated ethenes.	2016	26941158
biocatalytic desulfurization capabilities of a mixed culture during non-destructive utilization of recalcitrant organosulfur compounds.	we investigated the biodesulfurization potential of a mixed culture ak6 enriched from petroleum hydrocarbons-polluted soil with dibenzothiophene (dbt) as a sulfur source. in addition to dbt, ak6 utilized the following compounds as sulfur sources: 4-methyldibenzothiophene (4-mdbt), benzothiophene (bt), and 4,6- dimethyldibenzothiophene (4,6-dm-dbt). none of these compounds supported the growth of ak6 as the sole carbon and sulfur source. ak6 could not grow on dibenzylsulfide (dbs) as a sulfur sou ...	2016	26973637
in vivo model for microbial invasion of tooth root dentinal tubules.	objective bacterial penetration of dentinal tubules via exposed dentine can lead to root caries and promote infections of the pulp and root canal system. the aim of this work was to develop a new experimental model for studying bacterial invasion of dentinal tubules within the human oral cavity. material and methods sections of human root dentine were mounted into lower oral appliances that were worn by four human subjects for 15 d. roots were then fixed, sectioned, stained and examined microsco ...	2016	27119760
different transcriptional responses from slow and fast growth rate strains of listeria monocytogenes adapted to low temperature.	listeria monocytogenes has become one of the principal foodborne pathogens worldwide. the capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of l. monocytogenes with different growth capacities at low temperatures. we determined the growth rate at 8°c of 110 strains of l. monocytogenes isolated from different food matrices. we identified a group of slow and fast strains according to thei ...	2016	26973610
the lona protease regulates biofilm formation, motility, virulence, and the type vi secretion system in vibrio cholerae.	the presence of the lon protease in all three domains of life hints at its biological importance. the prokaryotic lon protease is responsible not only for degrading abnormal proteins but also for carrying out the proteolytic regulation of specific protein targets. posttranslational regulation by lon is known to affect a variety of physiological traits in many bacteria, including biofilm formation, motility, and virulence. here, we identify the regulatory roles of lona in the human pathogen vibri ...	2016	26755629
biotechnological production of limonene in microorganisms.	this mini review describes novel, biotechnology-based, ways of producing the monoterpene limonene. limonene is applied in relatively highly priced products, such as fragrances, and also has applications with lower value but large production volume, such as biomaterials. limonene is currently produced as a side product from the citrus juice industry, but the availability and quality are fluctuating and may be insufficient for novel bulk applications. therefore, complementary microbial production ...	2016	26915992
characterization of sxt/r391 integrative and conjugative elements in proteus mirabilis isolates from food-producing animals in china.	sxt/r391 integrative and conjugative elements (ices) were detected in 8 out of 125 proteus mirabilis isolates from food-producing animals in china. whole-genome sequencing revealed that seven ices were identical to icepmijpn1, carrying the cephalosporinase gene blacmy-2. another one, designated icepmichn1, carried five resistance genes. all eight ices could be transferred to escherichia coli via conjugation. the results highlight the idea that animal farms are important reservoir of the sxt/r391 ...	2016	26824957
detection of substrate-dependent conformational changes in the p450 fold by nuclear magnetic resonance.	cytochrome p450 monooxygenases typically catalyze the insertion of one atom of oxygen from o2 into unactivated carbon-hydrogen and carbon-carbon bonds, with concomitant reduction of the other oxygen atom to h2o by nad(p)h. comparison of the average structures of the camphor hydroxylase cytochrome p450(cam) (cyp101) obtained from residual dipolar coupling (rdc)-restrained molecular dynamics (md) in the presence and absence of substrate camphor shows structural displacements resulting from the ess ...	2016	26911901
characterization of triclosan metabolism in sphingomonas sp. strain yl-jm2c.	triclosan (tcs) is one of the most widespread emerging contaminants and has adverse impact on aquatic ecosystem, yet little is known about its complete biodegradation mechanism in bacteria. sphingomonas sp, strain yl-jm2c, isolated from activated sludge of a wastewater treatment plant, was very effective on degrading tcs. response surface methodology (rsm) was applied to optimize the conditions like temperature and ph. from rsm, the optimal tcs degradation conditions were found to be 30 °c and p ...	2016	26912101