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evolutionary relationships among gamma-carboxymuconolactone decarboxylases.gamma-carboxymuconolactone decarboxylase (ec 4.1.1.44) from azotobacter vinelandii resembled the isofunctional enzymes from acinetobacter calcoaceticus and pseudomonas putida. all three decarboxylases appeared to be hexamers formed by association of identical subunits of about 13,300 daltons. the a. vinelandii and p. putida decarboxylases cross-reacted immunologically with each other, and the nh2-terminal amino acid sequences of the enzymes differed in no more than 7 of the first 36 residues. in ...19816783616
transfer of nitrogen fixation genes into pseudomonas putida isolated from finnish tundra soil.the plasmid prd1 containing the nif genes from klebsiella pneumoniae was transferred by conjugation from escherichia coli to pseudomonas putida isolated from the tundra soil. 6-cyanopurine, acetylene reduction and immunological tests showed that the nif genes were not expressed in p. putida. existence of the nif genes in p. putida transconjugants was detected by transferring them to e. coli c600, which does not fix nitrogen. existence of the nif genes in e. coli c600 transconjugants was detected ...19816798929
isolation of two lipoamide dehydrogenases from pseudomonas putida grown on valine. 19816895028
control of meta-cleavage degradation of 4-hydroxyphenylacetate in pseudomonas putida.synthesis of enzymes of the 4-hydroxyphenylacetate meta-cleavage pathway was studied in pseudomonas putida wild-type strain p23x1 (ncib 9865) and mutant strains which had either structural or regulatory gene mutations. induction studies with mutant strains each defective in an enzyme of the pathway showed that 4-hydroxyphenylacetate induced the hydroxylase and that 3,4-dihydroxyphenylacetate induced the 2,3-oxygenase, aldehyde dehydrogenase, isomerase, decarboxylase, and hydratase. this showed t ...19816895079
synthesis of d-cysteine from 3-chloro-d-alanine and hydrogen sulfide by 3-chloro-d-alanine hydrogen chloride-lyase (deaminating) of pseudomonas putida. 19816791643
cyclic adenosine 3',5'-monophosphate levels in pseudomonas putida and pseudomonas aeruginosa during induction and carbon catabolite repression of histidase synthesis.inducibility of histidase (histidine ammonia-lyase, ec 4.3.1.3) in pseudomonas putida and pseudomonas aeruginosa was observed to be strongly affected by succinate-provoked catabolite repression, but this did not occur as a consequence of reduced intracellular cyclic adenosine 3',5'-monophosphate levels, and repression could not be alleviated by exogenously added cyclic adenosine 3,'5'-monophosphate. milder repression of histidase by lactate was also not reversed by the addition of cyclic adenosi ...19816259129
properties of six pesticide degradation plasmids isolated from alcaligenes paradoxus and alcaligenes eutrophus.biophysical and genetic properties of six independently isolated plasmids encoding the degradation of the herbicides 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid are described. four of the plasmids, pjp3, pjp4, pjp5, and pjp7, had molecular masses of 51 megadaltons, belonged to the incp1 incompatibility group, and transferred freely to strains of escherichia coli, rhodopseudomonas sphaeroides, rhizobium sp., agrobacterium tumefaciens, pseudomonas putida, pseudomonas flu ...19816257648
molecular cloning of gene xyls of the tol plasmid: evidence for positive regulation of the xyldegf operon by xyls.the xyldegf operon and the regulatory gene xyls of the tol plasmid found in pseudomonas putida mt-2 were cloned onto escherichia coli vector plasmids. a 9.5-kilobase fragment, derived from the tol segment of ptn2 deoxyribonucleic acid, carried the xyl genes d, e, g, and f, which encode toluate oxygenase, catechol 2,3-oxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, and 2-hydroxymuconic semialdehyde hydrolase, respectively. the enzymes were noninducible unless a 3-kilobase psti fragment, d ...19816271729
purification and properties of protocatechuate 3,4-dioxygenase from pseudomonas putida. a new iron to subunit stoichiometry.protocatechuate dioxygenase has been isolated from pseudomonas putida. this new species of protocatechuate dioxygenase has been characterized and compared with the enzyme from pseudomonas aeruginosa. the enzyme reported here has visible absorption, circular dichroism, electron paramagnetic resonance, and raman spectroscopic properties virtually identical to those for protocatechuate dioxygenase from p. aeruginosa. however, the molecular weight and iron:subunit stoichiometry differ. protocatechua ...19816273403
mössbauer studies on the active fe ... [2fe-2s] site of putidamonooxin, its electron transport and dioxygen activation mechanism.putidamonooxin, the oxygenase of a 4-methoxybenzoate monooxygenase enzyme system, catalyzes the oxidative o-demethylation of the substrate 4-methoxybenzoate in conjunction with the nadh:putidamonooxin oxidoreductase. putidamonooxin is a conjugated iron-sulfur protein which needs iron ions as cofactors for its enzymatic activity. putiamonooxin was isolated from pseudomonas putida, which was grown on a 57fe-enriched culture medium. thus putidamonooxin was enriched in vivo with 57fe up to about 80% ...19816276173
glutamine synthetase of pseudomonads: some biochemical and physicochemical properties.the glutamine synthetases from several pseudomonas species were purified to homogeneity, and their properties were compared with those reported for the enzymes from escherichia coli and other gram-negative bacteria. the glutamine synthetase from pseudomonas fluorescens was unique because it was nearly precipitated quantitatively as a homogeneous protein during dialysis of partially purified preparations against buffer containing 10 mm imidazole (ph 7.0) and 10 mm mncl2. the glutamine synthetases ...19816111557
an electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from pseudomonas putida. 19816273164
mechanism of urocanase as studied by deuterium isotope effects and labeling patterns.nicotinamide adenine dinucleotide (nad) dependent urocanase (4'-imidazolone-5'-propionate hydro-lyase, ec 4.2.1.49) from pseudomonas putida was found to catalyze an exchange reaction between solvent and the 4'-hydrogen of urocanate or imidazolepropionate at a rate faster than that of overall deuterium was compared to unlabeled urocanate as a substrate, no isotope rate effect was noted. for examination of the possibility of an nad+-mediated intramolecular hydride transfer of the 4'-hydrogen to a ...19816110440
in vitro antimicrobial activity of ceftizoxime against glucose-nonfermentative gram-negative rods.ceftizoxime, a new cephalosporin, was active against pseudomonas cepacia, flavobacterium meningosepticum, alcaligenes faecalis, and acinetobacter calcoaceticus and was more potent against pseudomonas aeruginosa and pseudomonas putida than was carbenicillin.19816269480
the respiratory system of pseudomonas putida: participation of cytochromes in electron transport. 19816269495
similar structures in gamma-carboxymuconolactone decarboxylase and beta-ketoadipate succinyl coenzyme a transferase.gamma-carboxymuconolactone decarboxylase (ec 4.1.1.44) and beta-ketoadipate succinyl coenzyme a transferase (ec 2.8.3.6) mediate different steps in the beta-ketoadipate pathway. antisera prepared against the pseudomonas putida transferase cross-reacted immunologically with the decarboxylase from the same organism. the transferase is formed by association of two nonidentical protein subunits. the nh2-terminal amino acid sequences of the two nonidentical transferase subunits resembled each other a ...19826172419
the tol plasmid is naturally derepressed for transfer.pseudomonas putida mt-2, formerly known as pseudomonas arvilla mt-2, which carries the wild-type tol plasmid, and p. putida strain ac37 carrying tol, were completely lysed by the pilus-adsorbing plasmid-specific bacteriophages pr4 and prd1. pseudomonas putida strain pps388, also harbouring the plasmid, was not lysed. in a p. putida mt-2 host, tol transferred 18-fold better on a surface (2.5 x 10(-1) transconjugants per donor h-1) than in liquid; when p. putida pps388 was the host, however, a fre ...19826134782
broad-host-range incp-4 plasmid r1162: effects of deletions and insertions on plasmid maintenance and host range.r1162 is an 8.7-kilobase (kb) broad-host-range replicon encoding resistance to streptomycin and sulfa drugs. in vitro deletion of 1.8-kb dna between coordinates 3.0 and 5.3 kb did not affect plasmid maintenance, but a tn1 insertion at coordinate 6.3 kb led to a recessive defect in plasmid maintenance. the only cis-acting region necessary for plasmid replication appears to lie between the tn1 insertion at coordinate 6.3 kb and a second tn1 insertion at coordinate 6.5 kb. all r1162 sequences betwe ...19826288654
spontaneous deletions in the tol plasmid pww20 which give rise to the b3 regulatory mutants of pseudomonas putida mt20.the size of the tol plasmid pww20 from pseudomonas putida mt20, as measured by analysis of agarose electrophoresis gels after restriction endonuclease hydrolysis, was 270-280 kilobase pairs (kb). during growth on benzoate, mt20 segregates strains carrying mutations in the plasmid regulatory gene xyls; these so-called b3 strains retain the ability to grow on m-xylene (mxy+) but do not grow on its metabolite m-toluate (mtol-) and have also lost the ability to transfer the plasmid (tra-). analysis ...19826288840
plasmid gene organization: naphthalene/salicylate oxidation.genes for naphthalene metabolism are localized on nah7, an 83-kilobase (kb) plasmid, in two gene clusters under salicylate control. polar mutations formed by insertion of the transposon tn5 permit detection of the transcription direction and the gene organization within two approximately 10-kb dna segments separated by a approximately 7-kb regulatory gene region. the gene cluster specifying conversion of naphthalene to salicylate lies near the left initiation of a 25-kb dna fragment a released b ...19826278499
identification of chromosomally integrated tol dna in cured derivatives of pseudomonas putida paw1.some plasmid-free tol- strains derived from pseudomonas putida paw1 (which carries the tol plasmid pww0) have a segment of tol dna located chromosomally. of three independently isolated strains, paw86 had an integrated tol segment of 16 kilobases and paw85 had two copies of this segment in different chromosomal locations, whereas the chromosomal dna of paw82 showed no homology with the tol plasmid. in cultures of the parental strain, it appears that a 56-kilobase tol dna segment is located chrom ...19826290457
use of buffered hypochlorite solution for disinfecting fibrescopes.the possible use of sodium hypochlorite solution buffered to ph 7.6 and containing 100 ppm available chlorine (avcl) for disinfecting fibrescopes was investigated. a flexible fibrescope experimentally contaminated with pseudomonas putida, mycobacterium fortuitum, or bacillus subtilis spores was effectively disinfected within 10 m in repeatedly and without any observable adverse effect on the instrument. the corrosive nature of buffered hypochlorite was investigated by immersing various fibrescop ...19826802880
d-malic enzyme of pseudomonas fluorescens.by the enrichment culture technique 14 gram-negative bacteria and two yeast strains were isolated that used d(+)-malic acid as sole carbon source. the bacteria were identified as pseudomonas putida, pseudomonas fluorescens, pseudomonas aeruginosa and klebsiella aerogenes. in cell-free extracts of p. fluorescens and p. putida the presence of malate dehydrogenase, d-malic enzyme (nad-dependent) and l-malic enzyme (nadp-dependent) was demonstrated. d-malic enzyme from p. fluorescens was purified. s ...19826804238
insights into the active site of the cytochrome p-450 haemoprotein family--a unifying concept based on structural considerations.1. cytochromes p-450cam of pseudomonas putida and p-450lm-2 of rabbit liver, and to a lesser extent also adrenocortical mitochondrial cytochromes p-450(11) beta and p-450scc, were investigated by active site-targeted reagents and by immunochemical techniques. the results of these studies and of the alignment of the amino acid sequences of cytochromes p-450cam and of phenobarbital-induced rat-liver p-450lm support the following conclusions. 2. cytochrome p-450 haemoproteins follow a common archit ...19826762774
[integration of the genome of the mu-like pseudomonas aeruginosa bacteriophage d3112 into plasmid rp4 and its hybrid plasmid transfer into pseudomonas putida and escherichia coli c600 bacteria].the genome of a mu-like bacteriophage d3112 specific for pseudomonas aeruginosa was integrated in vivo into the rp4 plasmid. the fact of integration has been proved by two experiments: 1. the loss of rp4 plasmid is accompanied by loss of d3112 prophage; 2. transfer of the plasmid by conjugation from pseudomonas aeruginosa into bacteria of other species - p. putida pgg1 or escherichia coli c600 leads to the occurrence of clones of these species which liberate phage capable of growing on the lawn ...19826799358
amino acid sequence of the pseudomonas putida cytochrome p-450. i. sequences of tryptic and clostripain peptides.in order to elucidate the complete amino acid sequence of pseudomonas putida cytochrome p-450, tryptic digestion was performed on the s-carboxymethylated enzyme. although cleavage did not occur at every lysyl and arginyl bond, 31 tryptic peptides ranging in size from 1 to 55 residues were isolated. these were sequenced by manual edman degradation and carboxypeptidase digestion. overlaps of some od these tryptic peptides were obtained by data obtained from partial edman degradation and amino acid ...19826752138
[preparation of protoplast-like structures of pseudomonas putida and their reversion to bacterial forms].a technique is proposed for the preparation of pseudomonas putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing mono-and divalent cations. pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 m) for 90 min at the ph 7.9 to 8.0 is a prerequisite for the formation of 'protoplasts'. under these conditions, the yield of 'protoplasts' reached 99.9% of the total cell number. the protoplast-like structures are capable of reversin ...19826755196
delta1-piperideine-2-carboxylate reductase of pseudomonas putida.pseudomonas putida metabolizes d-lysine to delta 1-piperideine-2-carboxylate and l-pipecolate. the second step of this catabolic pathway is catalyzed by delta 1-piperideine-2-carboxylate reductase. this enzyme was isolated and purified from cells grown on dl-lysine as substrate. the enzyme was very unstable, resulting in low recovery of activity and low purity after a six-step purification procedure. the enzyme had a ph optimum of 8.0 to 8.3. the km values for delta 1-piperideine-2-carboxylate a ...19826801013
monoclonal antibodies against pseudomonas aeruginosa outer membrane antigens: isolation and characterization.hybridomas secreting monoclonal antibodies specific for pseudomonas aeruginosa outer membrane antigens were isolated. one of the antibodies was highly specific for the o antigen of the lipopolysaccharide of international antigen typing scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotypes. this monoclonal antibody was able to agglutinate heat-killed bacterial cells as well as lipopolysacc ...19826809625
[expression of the genome of mu-like phage d3112 specific for pseudomonas aeruginosa in escherichia coli and pseudomonas putida cells].the behavior of escherichia coli cells carrying rp4 plasmid which contains the genome of a mu-like d3112 phage specific for pseudomonas aeruginosa was studied. two different types of d3112 genome expression were revealed in e. coli. the first is bp4-dependent expression. in this case, expression of certain d3112 genes designated as "kil" only takes place when rp4 is present. as a result, cell division stops at 30 degrees c and cells form filaments. cell division is not blocked at 42 degrees c. t ...19826811373
roles of cysteine sulfinate and transaminase on in vitro dark reversion of urocanase in pseudomonas putida.urocanase is inactivated in intact cells of pseudomonas putida and photoactivated by brief exposure of the cells to the uv radiation in sunlight. the dark reversion (inactivation) in vitro is explained by the formation of a sulfite-nad adduct. our objective was to investigate the dark reversion in vivo. various compounds were added to p. putida cells, and the reversion was measured, after sonication, by comparison of the activity before and after uv irradiation. sulfite, cysteine sulfinate, and ...19826124532
the reaction of 4-deoxy-4-fluoro-d-glucose with an outer membrane protein of pseudomonas putida. 19827117530
amino acid sequence of the pseudomonas putida cytochrome p-450. ii. cyanogen bromide peptides, acid cleavage peptides, and the complete sequence. 19827130171
4-hydroxy-4-methyl-2-ketoglutarate aldolase from pseudomonas putida. 19827154956
3-chloro-d-alanine chloride-lyase (deaminating) of pseudomonas putida cr 1.1. purification and characterization of a novel enzyme occurring in 3-chloro-d-alanine-resistant pseudomonads.a novel enzyme catalyzing cleavage of 3-chloro-d-alanine to pyruvate, ammonia, and chloride ion is distributed in some pseudomonads which have a resistance to high concentrations of 3-chloro-d-alanine. pseudomonas putida cr 1-1 (aku 867) was found to have the highest activity of enzyme, which was inducibly formed by the addition of 3-chloro-d-alanine to the medium. the enzyme, tentatively called 3-chloro-d-alanine chloride-lyase, was purified from p.l putida cr 1-1 in seven steps. after the last ...19826815180
[plasmid pbs241 of pseudomonas putida controlling biphenyl degradation]. 19827140506
stimulation of 3,4-dichloroaniline mineralization by aniline.mineralization of free and of humus-bound 3,4-dichloroaniline (dca) by a pseudomonas putida strain isolated by analog enrichment was greatly enhanced in the presence of aniline. the addition of aniline to soil that contained 0.2 to 100 micrograms of dca per g in free or in humus-bound form increased the mineralization rates of dca severalfold. within the concentration ranges tested, absolute mineralization of dca per unit time was positively correlated with both increasing dca and increasing ani ...19827138005
active transport of benzoate in pseudomonas putida.benzoate uptake in pseudomonas putida is mediated by an active transport system capable of accumulating benzoate against a 150-fold concentration gradient when subsequent metabolism is blocked by mutation. initial benzoate transport rates are inhibited by cccp, sodium azide, arsenate and dccd. uptake is stimulated by including a respirable carbon source during preincubation of the bacteria. the initial uptake rate and the atp pool levels are not correlated and no periplasmic components were foun ...19827142957
comparison of kinetics of active tetracycline uptake and active tetracycline efflux in sensitive and plasmid rp4-containing pseudomonas putida.membrane vesicles prepared from tetracycline-sensitive cells of pseudomonas putida took up tetracycline by an active transport system with an apparent km of 2.5 mm and a vmax of 50 nmol min-1 mg protein-1. in contrast, resistance determinant rp4-containing p. putida had an active high-affinity efflux system for tetracycline with a km of 2.0 to 3.54 microm and a vmax of 0.15 nmol min-1 mg protein-1. thus, the efflux system of tetracycline-resistant p. putida(rp4) had an average of 1,000-fold grea ...19827118827
tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: enzyme regulation and dna structure.wr211 and wr216 are derivatives of halobenzoate-degrading pseudomonas sp. strain b13 into which the 117-kilobase tol degradative plasmid pww0 has been transferred from pseudomonas putida mt-2. wr211 has lost the ability to grow on the tol-specific substrate m-xylene but retains the ability to grow on its metabolite, m-toluate. an analysis of the induction of enzymes was consistent with wr211 carrying a nonfunctional regulatory gene, xy1r, wr216 is a spontaneous derivative of wr211 which grows on ...19827061391
tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: prevention of meta pathway.the hybrid pathway for chlorobenzoate metabolism was studied in wr211 and wr216, which were derived from pseudomonas sp. b13 by acquisition of tol plasmid pww0 from pseudomonas putida mt-2. chlorobenzoates are utilized readily by these strains when meta cleavage of chlorocatechols is suppressed. when wr211 utilizes 3-chlorobenzoate (3cb), the expression of catechol 2,3-dioxygenase (c23o) and the catabolic activities for chloroaromatics via the ortho pathway coexist as a consequence of inactivati ...19827061393
[catalase activity of a pseudomonas putida strain oxidizing arsenic].trivalent arsenic ions added to the medium stimulated the catalase activity of a pseudomonas putida 18 culture correlating with the rate of arsenic oxidation. as soon as the process of arsenic oxidation stopped, the activity of catalase decreased. apparently, the production of h2o2, its decomposition by catalase and oxidation of as(iii) to as(v) by the formed oxygen are a device for detoxication of trivalent arsenic acquired by the cell in the course of evolution. therefore, oxidation of trivale ...19827070307
preliminary crystallographic data on cytochrome p-450cam.the cytochrome p-450cam monooxygenase from pseudomonas putida has been crystallized in three forms designated orthorhombic i, tetragonal i, and orthorhombic ii. conditions and parameters of crystal growth are presented along with the preliminary x-ray diffraction data including heavy atom derivatives.19827107613
stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of pseudomonas putida pp3: evidence for two different dehalogenation mechanisms.pseudomonas putida pp3 grew on dl-2-monochloropropionate (2mcpa) with a release of chloride ions consistent with the dechlorination of both isomers. the organism grew on either d- or l-2mcpa. dehalogenase activity in cell-free extracts showed that both d- and l-2mcpa were dehalogenated. pseudomonas putida pp3 contains two dehalogenases, and studies with the separated enzymes revealed that the fraction i enzyme used both d- and l-2mcpa, the rate of dechlorination of l-2mcpa being 80% of the rate ...19827142958
subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from pseudomonas arvilla c-1.benzoate-1,2-dioxygenase system from pseudomonas arvilla c-1 consists of two protein components, benzoate-1,2-dioxygenase reductase and benzoate-1,2-dioxygenase (yamaguchi, m., and fujisawa, h. (1980) j. biol. chem. 255, 5058-5063). benzoate-1,2-dioxygenase exhibited two protein bands (alpha and beta) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their molecular weights were estimated to the 50,000 and 20,000, respectively. the intensities of protein staining on polyacrylamid ...19827130163
the metabolism of cyclohexanecarboxylic acid and 3-cyclohexenecarboxylic acid by pseudomonas putida.a strain of pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. a coa-mediated beta-oxidation pathway was induced for the metabolism of the compound. the organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in the presence of 3-cyclohexenecarboxylic acid were induced to metabolize cyclohexanecarboxylic acid, benzoic acid, and catechol. evidence is presented that 3-cyclohexenecarboxylic ac ...19827168830
sensitivity of 341 non-fermentative gram-negative bacteria to seven beta-lactam antibiotics.susceptibility of 341 isolates of non-fermentative gram-negative bacteria to carbenicillin, piperacillin, cefoperazone, moxalactam, cefotaxime, ceftizoxime, and n-formimidoyl thienamycin was determined by the agar dilution and disc diffusion methods. piperacillin was the most active agent against pseudomonas aeruginosa, thienamycin the most active against pseudomonas fluorescens and pseudomonas putida, and moxalactam the most active against pseudomonas maltophilia. piperacillin and thienamycin w ...19827173181
structure-activity relationships in microbial transformation of phenols.the second-order rate constants for the microbial transformation of a series of phenols were correlated with the physicochemical properties of the phenols. the compounds studied were phenol, p-methylphenol, p-chlorophenol, p-bromophenol, p-cyanophenol, p-nitrophenol, p-acetylphenol, and p-methoxyphenol. phenol-grown cells of pseudomonas putida u transformed these compounds. microbial transformation rate constants ranged from (1.5 +/- 0.99) x 10 liter . organism . h for p-cyanophenol to (7.0 +/- ...198216346051
a model for noninhibitory microbial growth.a model for noninhibitory microbial growth has been developed which is superior to the monod model in that it can predict the decline in steady-state growth yields at both the slow and the fast specific growth rates. the model parameters are evaluated from data obtained for steady-state, phenol-limited pseudomonas putida growth using a conventional 1-dm(3) cheniostat. the model also has been successfully applied to mor and fiechter's data for cheniostat yeast cultures.198218546099
linalyl acetate is metabolized by pseudomonas incognita with the acetoxy group intact.metabolism of linalyl acetate by pseudomonas incognita isolated by enrichment culture on the acyclic monoterpene alcohol linalool was studied. biodegradation of linalyl acetate by this strain resulted in the formation of linalool, linalool-8-carboxylic acid, oleuropeic acid, and delta-4-acetoxy-4-methyl hexenoic acid. cells adapted to linalyl acetate metabolized linalyl acetate-8-aldehyde to linalool-8-carboxylic acid, linalyl acetate-8-carboxylic acid, delta-4-acetoxy-4-methyl hexenoic acid, an ...198316346182
regiochemistry of camphor analog oxidation by pseudomonas putida.pseudomonas putida cooxidized norcamphor and pericyclocamphanone to hydroxylated and lactonized products during growth on camphor. norcamphor was hydroxylated at the 5 position, similar to the corresponding process in camphor, but pericyclocamphanone was oxidized at the 6 position. we conclude that the regiochemistry of the hydroxylation may be substrate controlled.198316346279
plasmid involvement in acyclic isoprenoid metabolism by pseudomonas putida.an organism identified as pseudomonas putida was found to utilize citronellol or geraniol as the sole carbon and energy source. the ability to degrade these acyclic isoprenols was associated with psrq50, a 50-megadalton transmissible plasmid.198316346325
iron-chelating compounds produced by soil pseudomonads: correlation with fungal growth inhibition.strains of pseudomonas putida, pseudomonas sp., and pseudomonas aeruginosa were examined for their ability to grow in the presence of the iron chelator, ethylenediamine-di-(o-hydroxyphenylacetic acid). in vitro fungal inhibition assays showed that the isolates varied in their ability to inhibit the growth of representative fungal plant pathogens. fungal inhibition in vitro was superior to that of previously reported pseudomonas sp. studies with fusarium oxysporum forma sp. lycopersici and a susc ...198316346334
selection of bacteria with favorable transport properties through porous rock for the application of microbial-enhanced oil recovery.this paper presents a bench-scale study on the transport in highly permeable porous rock of three bacterial species-bacillus subtilis, pseudomonas putida, and clostridium acetobutylicum-potentially applicable in microbial-enhanced oil recovery processes. the transport of cells during the injection of bacterial suspension and nutrient medium was simulated by a deep bed filtration model. deep bed filtration coefficients and the maximum capacity of cells in porous rock were measured. low to interme ...198316346414
initiation of activation of a preemergent herbicide by a novel alkylsulfatase of pseudomonas putida fla.the activation of the preemergent herbicide 2-(2,4-dichlorophenoxy)ethyl sulfate (crag herbicide) is initiated by soil microorganisms that are presumed to act by removing the ester sulfate group via some type of sulfatase enzyme. an enrichment technique with the herbicide as the sole source of sulfur led to the isolation of several pure cultures that could produce 2-(2,4-dichlorophenoxy)ethanol from the herbicide. one of these, a strain of pseudomonas putida, was particularly active. polyacrylam ...198316346434
synthesis of d-cysteine-related amino acids by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1.using the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1, s-methyl-, s-ethyl-, s-n-propyl-, s-n-butyl-, s-phenyl-, s-benzyl-, s-(2,3-dihydroxypropyl)- and s-(2-hydroxyethyl)-d-cysteine were synthesized from 3-chloro-d-alanine and methyl-, ethyl-, n-propyl-, n-butyl-, phenyl-, benzyl-mercaptans, alpha-thioglycerol and 2-mercaptoethanol, respectively. the enzymatically synthesized d-cysteine-related amino acids were isolated from the large scale react ...19836838588
camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by pseudomonas putida.previously, pseudomonas putida was shown to degrade (+)-camphor, and cleavage of the first ring of the bicyclic structure involved two monooxygenases (a hydroxylase and a ring oxygen-inserting enzyme), a dehydrogenase, and spontaneous cleavage of an unstable oxygenation product (lactone). cleavage of the second ring was not demonstrated but was assumed also to occur by ring oxygen insertion, since the predicted oxygenation product was extracted from whole-cell incubation systems. our investigati ...19836848481
redox potential of the cytochrome c in the flavocytochrome p-cresol methylhydroxylase.the redox potential of the cytochrome c in 5 flavocytochrome c proteins, all p-cresol methylhydroxylases purified from species of pseudomonas, was measured. all gave similar values ranging from 226-250 mv. two of the enzymes, from pseudomonas putida nc1b 9866 and nc1b 9869, were resolved into their flavoprotein and cytochrome subunits and the redox potentials of the isolated cytochrome c subunits measured. the values for these were 60-70 mv below those for the whole enzymes but, in both cases, r ...19836309572
properties and roles of bacterial symbionts of polyvinyl alcohol-utilizing mixed cultures.from several polyvinyl alcohol (pva)-utilizing mixed cultures, two component bacterial strains essential for pva utilization were isolated, and their properties and roles in pva utilization were studied. each pair of essential component strains consisted of a type i strain, which produced a pva-degrading enzyme and constituted the predominant population of the mixed culture in pva, and a type ii strain, which produced a certain growth stimulant for the former strain. all of the type i strains we ...19836639015
heme binding and substrate-protected cysteine residues in p-450cam.reactions of the pseudomonas putida cytochrome p-450-substrate complex or enzyme alone with 14c-labeled iodoacetic acid have been investigated at ph 7.0. after subsequent conversion of all of the cysteine residues to s-beta-carboxymethylcysteinyl residues, tryptic peptides of the derivative were separated by either high performance liquid chromatography or two dimensional electrophoresis, and their amino acid compositions and partial sequences were determined. all but cysteine residue-134 reacte ...19836639664
[degradation of 3-chlorobenzoic acid by a pseudomonas putida strain].a pseudomonas putida strain 87 capable of assimilating 3-chlorobenzoic acid as a sole source of carbon and energy (3cba+) was isolated. treatment with mitomycin c eliminated the 3cba+ phenotype in 1% of cells in the population. the 3cba+ phenotype was transferred at a low frequency in the process of conjugation to other bacteria belonging to the genus pseudomonas. determinants localized on the plasmid are presumed to be responsible for the capability to assimilate 3-chlorobenzoic acid. a scheme ...19836664313
synthesis of s-(carboxymethyl)-d-cysteine by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1.s-(carboxymethyl)-d-cysteine, which is an important component of semisynthetic cephalosporin, mt-141, was enzymatically synthesized. s-(ethoxy-carbonyl-methyl)-d-cystein was synthesized from 3-chloro- d-alanine and ethyl thioglycolate by the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1 and subsequently hydrolyzed by alkali. the synthesized s-(carboxymethyl)-d-cysteine was isolated from a large scale reaction mixture and identified physicochemicall ...19836679711
properties and function of malate enzyme from pseudomonas putida.malate enzyme (l-malate: nadp+ oxidoreductase (oxalacetate-decarboxylating, ec 1.1.1.40)) has been purified from pseudomonas putida to 99 per cent homogeneity by heat, ammonium suphate fractionation, gel filtration and anion exchange chromatography. sodium dodecylsulphate-(sds)-polyacrylamide disc gel electrophoresis analysis showed an approximate tetrameric subunit with a molecular weight of 52,000. the purified enzyme showed a ph optimum between 8.0 and 8.5 (for tris-hcl buffer) and required b ...19836673742
expression of the argf gene of pseudomonas aeruginosa in pseudomonas aeruginosa, pseudomonas putida, and escherichia coli.r' plasmids carrying argf genes from pseudomonas aeruginosa strains pao and pac were transferred to pseudomonas putida argf and escherichia coli argf strains. expression in p. putida was similar to that in p. aeruginosa and was repressed by exogenous arginine. expression in e. coli was 2 to 4% of that in p. aeruginosa. exogenous arginine had no effect, and there were no significant differences between argr' and argr strains of e. coli in this respect.19836403512
chromogenic identification of genetic regulatory signals in bacillus subtilis based on expression of a cloned pseudomonas gene.a method to isolate fragments of dna that promote gene expression in bacillus subtilis is described. the system is based on production of catechol 2,3-dioxygenase [cato2ase; catechol:oxygen 2,3-oxidoreductase (decyclizing), ec 1.13.11.2] encoded by the pseudomonas putida tol plasmid gene xyle. the gene was transferred to ab. subtilis/escherichia coli plasmid vector to construct ptg402. although xyle is functionally expressed in e. coli, cato2ase is not detected in b. subtilis unless a fragment o ...19836405380
[molecular dna-dna hybridization in pseudomonas fluorescens and pseudomonas putida].taxonomic analysis was carried out and dna-dna homology studied in cultures of subgroups formed by numerical classification of 124 pseudomonas fluorescens and p. putida strains. the gc content in the dna of strains belonging to p. fluorescens subgroups varied from 61.2 to 64.5%, and their dna-dna homology with the type strain p. fluorescens atcc 13525 was 24 to 83%. the lowest genome relatedness with the type culture was found in biotype c of p. fluorescens and p. aureofaciens strains. the gc c ...19836413827
the microbial oxygenation of the benzylisoquinoline alkaloid laudanosine.the microbial transformation of the benzylisoquinoline alkaloid laudanosine by a strain of pseudomonas putida gives a metabolite in which o-demethylation of 1 methoxyl group of ring c, and introduction of 1 ketonic oxygen at c9 and 1 phenolic oxygen at ring c have occurred. also, o-methylcoripalline is formed in this transformation.19836641902
ligand binding to heme proteins. an evaluation of distal effects.the o2, co, and alkyl isocyanide-binding properties of a variety of vertebrate and invertebrate heme proteins have been compared in detail to those of protoheme mono-3-(1-imidazoyl)-propylamide monomethyl ester in aqueous suspensions of soap micelles. the proteins examined include: cytochrome p-450cam from pseudomonas putida, beef heart cytochrome c oxidase, yeast cytochrome c peroxidase, alpha and beta subunits of human hemoglobin, sheep hemoglobin, carp hemoglobin, sperm whale myoglobin, horse ...19836643477
rapid reaction studies on the oxygenation reactions of catechol dioxygenase.the reaction of oxygen with catechol 1,2-dioxygenase from pseudomonas arvilla atcc 23974 in complex with catechol, 4-methylcatechol, and 4-fluorocatechol has been studied using single turnover stopped flow spectrophotometry. two sequential enzyme intermediates have been resolved and their visible spectra characterized by computer-assisted methods. these intermediates are spectrally similar to those observed in a similar study with protocatechuate dioxygenase (bull, c., ballou, d. p., and otsuka, ...19836643492
complete nucleotide sequence of the metapyrocatechase gene on the toi plasmid of pseudomonas putida mt-2.metapyrocatechase which catalyzes the oxygenative ring cleavage of catechol to form alpha-hydroxymuconic epsilon-semialdehyde is encoded by the xyle gene on the tol plasmid of pseudomonas putida mt-2. we have cloned the xyle region in escherichia coli and determined the nucleotide sequence of the dna fragment of 985 base pairs around the gene. the fragment included only one open translational frame of sufficient length to accommodate the enzyme. the predicted amino acid sequence consisted of 307 ...19836826546
steroid 21-hydroxylase (cytochrome p-450) from porcine adrenocortical microsomes: microsequence analysis of cysteine-containing peptides.the steroid 21-hydroxylase cytochrome p-450 from porcine adrenocortical microsomes was purified to homogeneity. the protein exhibited two nh2-terminal sequences, one of which was identical with the first but lacking the nh2-terminal methionine. the sequence was extremely hydrophobic but had little homology to the 17 alpha-hydroxylase/c17,20-lyase isolated from neonatal porcine testes or to rat or rabbit liver microsomal cytochromes p-450. the cysteine-containing fragments of the s-carboxymethyla ...19836830757
enzymes of the beta-ketoadipate pathway in pseudomonas putida: kinetic and magnetic resonance studies of the cis,cis-muconate cycloisomerase catalyzed reaction.steady-state kinetic analysis of the divalent metal ion requiring cis,cis-muconate cycloisomerase catalyzed interconversion of cis,cis-muconate and (+)-muconolactone obeys michaelis-menten kinetics and the haldane relationship from ph 6.2 to 8.3. the ph vs. kcat/km profiles suggest free-enzyme apparent pka values of 6.2 and 7.4: the reciprocal behavior of the data with respect to the latter pka value is consistent with base-acid catalysis by the enzyme involving proton removal from the lactone a ...19836652062
enzymes of the beta-ketoadipate pathway in pseudomonas putida: primary and secondary kinetic and equilibrium deuterium isotope effects upon the interconversion of (+)-muconolactone to cis,cis-muconate catalyzed by cis,cis-muconate cycloisomerase.primary and secondary kinetic and secondary equilibrium deuterium isotope effect studies on the cis,cis-muconate cycloisomerase catalyzed interconversion of cis,-cis-muconate (ccm) and (+)-muconolactone (ml) have been performed. the primary and solvent kinetic deuterium isotope effects upon vmax for the reactions of (+)-[5r-2h]muconolactone in water (hoh) and (+)-muconolactone in deuterium oxide (dod) to form cis,cis-muconate are about 2.5-2.6 with the heavier isotopic reactions being the slower ...19836652063
[cytotoxic effect of methionine-gamma-lyase on neoplastic cells in culture].the influence of methionine-gamma-lyase from pseudomonas putida on dna synthesis by caov and l-8 cell lines has been studied. the agent has been demonstrated to inhibit the incorporation of 3h-thymidine into l-8 cell line and to have no effect on caov cells.19836850097
immunological relatedness of histidine ammonia-lyases from some species of pseudomonas: taxonomic implication.1. histidine ammonia-lyases (histidase ec 4.3.1.3) from pseudomonas testosteroni ncib 10808 and pseudomonas putida ncib 10807 were purified and specific antibody was raised to each separately in a rabbit. 2. immunological cross-reactions of each antibody to histidine ammonia-lyases from various species of pseudomonas were examined by the enzyme inhibition test. 3. the immunological data obtained suggest that these pseudomonas species can be classified into three groups. these cross-reactions ten ...19836862094
[improved procedure for isolation and purification of methionine gamma-lyase from pseudomonas putida].an improved, simplified and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme--methionine gamma-lyase from pseudomonas putida. the method includes five steps instead of seven steps in previous procedure with a good yield of the enzyme. the purified enzyme was shown to be homogeneous by the criteria of disc gel electrophoresis. the highly homogeneous preparations of the enzyme exhibited the absorption maxima at 280 and 420 nm. the detailed studies on ...19836623989
structure of iron superoxide dismutase from pseudomonas ovalis at 2.9-a resolution.the three-dimensional structure of the iron-containing superoxide dismutase (ec 1.15.1.1) from pseudomonas ovalis has been determined at 2.9-a resolution by the method of multiple isomorphous replacement. the molecule is a dimer of two identical subunits with the iron atom per monomer. the conformation of the enzyme is completely different from that of the eukaryotic copper-zinc superoxide dismutase. each subunit consists of about 50% alpha-helix plus three strands of antiparallel pleated sheet. ...19836575382
plasmid-encoded regulation of colicin e1 gene expression.a plasmid-encoded factor that regulates the expression of the colicin e1 gene was found in molecular cloning experiments. the 2,294-base-pair avaii fragment of the colicin e1 plasmid (cole1) carrying the colicin e1 structural gene and the promoter-operator region had the same information with respect to the repressibility and inducibility of colicin e1 synthesis as the original cole1 plasmid. an operon fusion was constructed between the 204-bp fragment containing the colicin e1 promoter-operator ...19836313603
[transformation of escherichia coli and different species of pseudomonas by a plasmid dna isolated from pseudomonas testosteroni].pseudomonas testosteroni uses testosterone as sole source of carbon. we were able to isolate an extrachromosomal dna from a strain of pseudomonas testosteroni and to obtain pseudomonas putida and aeruginosa and escherichia coli transformants catabolizing testosterone.19836416627
mutations affecting lipoamide dehydrogenases of pseudomonas putida.pseudomonas putida grown on valine produces two lipoamide dehydrogenases, lpd-glu (mr, 56,000 and lpd-val (mr, 49,000). the 49,000-dalton protein is used by p. putida for branched-chain keto acid dehydrogenase, whereas the 56,000-dalton protein is presumably used for pyruvate and 2-ketoglutarate dehydrogenases. the objective of this study was to isolate and characterize mutants of p. putida with mutations affecting lipoamide dehydrogenases in order to study the relationship of these two proteins ...19836185468
integration of r91-5::tn501 into the pseudomonas putida ppn chromosome and genetic circularity of the chromosomal map.derivatives of the pseudomonas aeruginosa plasmid r91-5, loaded with the transposon tn501, were transferred to p. putida ppn. over 90% of exconjugants, which arose at a frequency of ca. 10(-6) per donor cell, exhibited high-frequency (greater than 10(-2) per donor cell) polarized transfer of chromosomal markers. in one instance it was demonstrated by transduction that the plasmid had been inserted into a gene required for serine biosynthesis. the integrated nature of the plasmid in this and othe ...19836294058
stereospecificity in meta-fission catabolic pathways.the stereospecificities of 2-keto-4-pentenoate hydratase and 4-hydroxy-2-ketovalerate aldolase were studied in escherichia coli, pseudomonas putida, and acinetobacter sp. hydration was stereospecific in all three; however, only p. putida and acinetobacter sp. showed stereospecificity in their aldolase reactions.19836345511
replication of derivatives of the broad host range plasmid rk2 in two distantly related bacteria.a 0.7-kb segment of the broad host range plasmid rk2 containing the replication origin of this plasmid will replicate in escherichia coli and pseudomonas putida when this segment is joined to a 1.8-kb region of rk2 designated traa*. the presence of another region of rk2, designated trfb, that previously was implicated in rk2 replication had no effect on the maintenance of the rk2 trfa*-oriv replicon in these two organisms. these observations indicate a requirement for a minimal account of inform ...19836346358
cloning and expression in escherichia coli of the naphthalene degradation genes from plasmid nah7.the genes encoding the enzymes responsible for conversion of naphthalene to 2-hydroxymuconic acid (naha through nahi) are contained on a 25-kilobase ecori fragment of an 85-kilobase nah plasmid of pseudomonas putida. these genes were cloned into the plasmid vectors pbr322 and rsf1010 to obtain the recombinant plasmids pkgx505 and pkgx511, respectively. to facilitate cloning and analysis, an nah7 plasmid containing a tn5 transposon in the salicylate hydroxylase gene (nahg) was used to derive the ...19836296054
molecular cloning of the pseudomonas carboxypeptidase g2 gene and its expression in escherichia coli and pseudomonas putida.the gene coding for carboxypeptidase g2 was cloned from pseudomonas sp. strain rs-16 into escherichia coli w5445 by inserting sau3a-generated dna fragments into the bamhi site of pbr322. the plasmid isolated, pnm1, was restriction mapped, and the position of the gene on the 5.8-megadalton insert was pinpointed by subcloning. the expression of carboxypeptidase in e. coli was 100-fold lower than in the pseudomonas sp. strain. when the cloned gene was subcloned into the pseudomonas vector pkt230 an ...19836358192
expression of naphthalene oxidation genes in escherichia coli results in the biosynthesis of indigo.a fragment of plasmid nah7 from pseudomonas putida ppg7 has been cloned and expressed in escherichia coli hb101. growth of the recombinant escherichia coli in nutrient medium results in the formation of indigo. the production of this dye is increased in the presence of tryptophan or indole. several bacteria that oxidize aromatic hydrocarbons to cis-dihydrodiols also oxidize indole to indigo. the results suggest that indigo formation is due to the combined activities of tryptophanase and naphthal ...19836353574
activity of the hybrid trp-lac (tac) promoter of escherichia coli in pseudomonas putida. construction of broad-host-range, controlled-expression vectors.a broad-host-range vector, pkt240, containing the structural gene (aph) for aminoglycoside phosphotransferase (aph), without promoter, has been constructed. insertion of dna fragments carrying promoters upstream of aph gene into the unique ecori site of this vector results in the expression of the aph gene and consequently the resistance of the host cells to streptomycin. the new vector has been used to show that the hybrid trp-lac (tac) promoter and the promoter of the laciq gene of escherichia ...19836323265
bacterial interference with urine osmolality measurements.we report bacterial interference with urine osmolality measurements using an instrument based on the principle of freezing point depression. although the exact nature of the interfering activity has not been defined, the phenomenon is associated with a bacterium, identified as pseudomonas putida, and is removed from the specimens by filtration at 0.45 micron. the bacteria led to osmometer dysfunction presumably by acting as a nidus for crystallization and preventing proper supercooling of specim ...19836616812
susceptibility of 324 nonfermentative gram-negative rods to 6 cephalosporins and azthreonam.susceptibility of 324 isolates of nonfermentative gram-negative bacteria to cephalothin, cefamandole, cefoxitin, ceftazidime, cefsulodin, and azthreonam was determined by agar dilution and disc diffusion techniques. with the exception of moraxella species, first- and second-generation cephalosporins were minimally active against nonfermenters tested. cefsulodin and azthreonam were mainly active against pseudomonas aeruginosa. in contrast, ceftazidime and ceftriaxone exhibited wider activity spec ...19836311492
cloning of genes for naphthalene metabolism in pseudomonas putida.plasmid pig7 dna cloned in pseudomonas putida with the broad-host-range vectors prk290 and pkt240 expresses the genes encoding nephthalene oxidation in the presence of the intermediate substrate, salicylate, or the gratuitous inducer, anthranilate. two operons, nahaf and nahgk, cloned from the ecori fragment a (25 kilobases) are under wild-type regulation by the nahr locus. deletion plasmids provide a restriction map of both operons. double transformants containing structural and regulatory cist ...19836311809
identification of the enol tautomer of imidazolone propionate as the urocanase reaction product.a fluorescent product was transiently formed during catalysis by urocanase from pseudomonas putida. the fluorophore showed an emission maximum at 430 nm when excited at 330 nm, essentially identical to that exhibited by the enol tautomer of imidazolone propionate. the keto isomer was not fluorescent under these conditions. in aqueous acid solutions where imidazolone propionate is relatively stable, an equilibrium mixture of tautomeric forms contained approximately 1% of the enol isomer. in ethan ...19836131645
proton coupling in the ligand-binding reaction of ferric cytochrome p-450 from pseudomonas putida.effects of ph on the ligand-binding reactions of ferric heme in cytochrome p-450 from pseudomonas putida (camphor 5-monooxygenase, ec 1.14.15.1) were studied by using cyanide, n-methylimidazole, pyridine, and ethylisocyanide as ligands. in all cases, affinity of the ferric heme for the ligand was found to increase as ph of the medium was raised from around 6 to 9. depending on the ligand, the increase was 10- to 1000-fold and the shapes of their ph-affinity curves were remarkably different. anal ...19836301381
structural characteristics of cytochrome p-450. possible location of the heme-binding cysteine in determined amino-acid sequences.computer-aided analyses were made of the complete amino-acid sequences of two p-450 species, the phenobarbital-inducible major p-450 of rat liver microsomes (p-450pb) and camphor-hydroxylating p-450 of pseudomonas putida (p-450cam). statistically significant homology was recognized between the two p-450 sequences, but these sequences were not related to those of other groups of hemoproteins, such as hemoglobins, peroxidases, and cytochrome c's and b's. two highly homologous regions, hr1 and hr2, ...19836307988
the iron content of iron superoxide dismutase: determination by anomalous scattering.the number of iron atoms in the dimeric iron-containing superoxide dismutase from pseudomonas ovalis and their atomic positions have been determined directly from anomalous scattering measurements on crystals of the native enzyme. to resolve the long-standing question of the total amount of iron per molecule for this class of dismutase, the occupancy of each site was refined against the measured bijvoet differences. the enzyme is a symmetrical dimer with one iron site in each subunit. the iron p ...19836135208
pseudomonas putida in transfused blood. 19846145996
novel system for recognizing and eliminating foreign dna in pseudomonas putida.derivatives of pqsr49 (r1162::tn1) containing cloned fragments of escherichia coli chromosomal dna are stable in e. coli but unstable in pseudomonas putida. similar derivatives containing p. putida chromosomal dna are stable in both species. instability is a consequence of plasmid loss during growth and is not due to death or inhibition of growth of plasmid-containing cells. average copy numbers per cell of the unstable hybrid plasmids are similar to that of pqsr49, indicating that instability i ...19846086582
resolution of 5-oxo-l-prolinase into a 5-oxo-l-proline-dependent atpase and a coupling protein.5-oxo-l-prolinase catalyzes a reaction in which the endergonic cleavage of 5-oxo-l-proline to form l-glutamate is coupled to the exergonic cleavage of atp to adp and pi. in the present research, the enzyme present in a strain of pseudomonas putida isolated from soil by enrichment culture was found to be composed of two protein components. neither component alone could catalyze the 5-oxoprolinase reaction, but the reaction was effectively catalyzed when they were mixed. one component (a) exhibite ...19846145710
the use of mudlac transposons as tools for vital staining to visualize clonal and non-clonal patterns of organization in bacterial growth on agar surfaces.when a histochemical stain for beta-galactosidase activity is applied to growth of gram-negative bacteria on agar medium, the pigmentation is non-uniform and capable of revealing internal colony organization into different cell types. use of an escherichia coli strain with a thermosensitive lac repressor indicates that colonies expand by addition of new cells at the periphery and that older cells which have synthesized beta-galactosidase early in development remain in the centre. mixed inocula o ...19846206197
escherichia coli and pseudomonas putida rna polymerases display identical contacts with promoters.methylation protection experiments with four promoters (p1 and p2 of the pbr322 plasmid, lacuv5 and lambda p0) have shown that the rna polymerases from escherichia coli and pseudomonas putida, while differing in the primary structure of the subunits involved in dna binding, display identical patterns of dna contacts. nor do these enzymes differ in covalent cross-linking patterns with a partially apurinized promoter. we conclude that the two rna polymerases have very similar structures of dna bin ...19846236350
nucleotide sequence of the promoter region of the xyldegf operon on tol plasmid of pseudomonas putida.the transcription initiation site of the xyldegf operon on the tol plasmid of pseudomonas putida mt-2 was determined in p. putida and in escherichia coli by s1 nuclease and reverse transcriptase mapping. the induced synthesis of mrna started at the same start point in both p. putida and e. coli, although the amount of mrna in e. coli cells was less than that in p. putida. the nucleotide sequence of the region surrounding the start point was also determined. the ribosome-binding site (rbs) comple ...19846092237
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