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properties of the iron--sulphur proteins of the benzene dioxygenase system from pseudomonas putida.a purification procedure was developed to stabilize the iron-sulphur proteins of the benzene dioxygenase system from pseudomonas putida. the intermediate electron-carrying protein has a mol. wt. of 12300 and possesses one (2fe--2s) cluster, whereas the terminal dioxygenase has a mol.wt. of 215300 and possesses two (2fe--2s) clusters. the order and stoicheiometry of electron transfer and of the whole system are described.1979435241
interaction of the chiral pyruvate analog, 2-keto-3-bromobutyrate, with pyruvate lyases. 2-keto-3-deoxygluconate-6-phosphate aldolase of pseudomonas putida.the enzyme 2-keto-3-deoxygluconate-6-p aldolase of pseudomonas putida is inactivated by one of the chiral forms of 2-keto-(3rs)-3-bromobutyric acid (bromoketobutyrate). the inactivation shows saturation kinetics and competition with pyruvate. the minimal inactivation half-time is 4 min and that concentration of bromoketobutyrate half-saturating the enzyme is 2 mm. (3rs)-[3-3h]bromoketobutyrate is catalytically detritiated during enzyme inactivation. a kinetic analysis of rates gave data consiste ...1979447683
raman spectrum of protocatechuate dioxygenase from pseudomonas putida. new low frequency bands. 1979454431
structural comparison of gamma-carboxymuconolactone decarboxylase and muconolactone isomerase from pseudomonas putida. 1979454663
nonidentical subunits of pyrocatechase from pseudomonas arvilla c-1. 1979475379
transport of c5 dicarboxylate compounds by pseudomonas putida.induced glutarate and 2-oxoglutarate uptake and transport by pseudomonas putida were investigated in whole cells and membrane vesicles, respectively. uptake of 2-oxoglutarate, but not glutarate, was against a concentration gradient to 1.7-fold greater than the initial extracellular concentration. membrane vesicles transported 2-oxoglutarate and glutarate against gradients to intramembrane concentrations fivefold greater than the initial extravesicle concentrations. the rates of transport of both ...1979479107
autoradiographic study of the localization and evolution of growth zones in bacterial colonies.incorporation of [3h]leucine in the bacteria of 18 to 48 h-old colonies of pseudomonas aeruginosa, pseudomonas putida, bacillus thuringiensis, staphylococcus aureus and escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. in colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria ...1979479831
[dechlorination of 4-chlorophenol following extradiolic ring cleavage by pseudomonas putida]. 1979483865
creatinine amidohydrolase (creatininase) from pseudomonas putida. purification and some properties. 1979500580
regulatory properties of histidase from pseudomonas putida. 1979518595
electron microscopy of some rock phosphate dissolving bacteria and fungi.bacteria pseudomonas striata, bacillus polymyxa, b. megaterium and b. pulvifaciens, and fungi aspergillus awamori, a. niger and penicillium digitatum dissolve tricalcium phosphate and, much less, mussorie and udaipur rock phosphate. the solubilizing power of fungi was higher than that of bacteria, the highest being with a. awamori and a. niger, and with p. striata. electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. the siz ...1979527907
local anesthetics block induction of the pseudomonas alk regulon.the local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in pseudomonas putida. procaine reversibly inhibited existing alkane hydroxylase activity. induction of a soluble aliphatic amidase activity was not affected. these results support the hypothesis that induction of the plasmid-determined alkane metabolic system in p. putida involves a membrane component(s).1979533765
regulation of membrane peptides by the pseudomonas plasmid alk regulon.pseudomonas putida strains carrying the plasmid alk genes will grow on n-alkanes. induced alk+ strains contain membrane activities for alkane hydroxylation and dehydrogenation of aliphatic primary alcohols. p. putida cytoplasmic and outer membranes can be separated by sucrose gradient centrifugation after disruption of cells by either mild detergent lysis or passage through a french press. both the membrane component of alkane hydroxylase and membrane alcohol dehydrogenase fractionated with the ...1979533768
coexistence of different pathways in the metabolism of n-propylbenzene by pseudomonas sp.pseudomonas desmolytica s449b1 and pseudomonas convexa s107b1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. the same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. a ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. together with ...1979543699
[purification and crystallization of a superoxide dismutase from pseudomonas putida]. 1979551636
direct hydroxylation in the biosynthesis of hydroxy fatty acid in lipid a of pseudomonas ovalis.it was found that pseudomonas ovalis iam 1177 had an abundance of hydroxy fatty acids such as 3-hydroxy-decanoic acid, 3-hydroxy-dodecanoic acid and 2-hydroxy-dodecanoic acid in the lipophilic part of the lipopolysaccharide fraction, which comprise 80% of total fatty acids. by using 18o2, it was shown that one oxygen atom from molecular oxygen was incorporated into 2-hydroxy-dodecanoic acid, but not into 3-hydroxy-decanoic acid. the incorporated oxygen atom was specifically located at the hydrox ...1979760794
fertility factors in pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors.the octane plasmid (oct) in pseudomonas putida strains has been shown to be transferred at low frequency. however, bacteria which had newly received this plasmid showed a transient increase in donor ability. using octane+ p. putida as the donor, the transfer of most chromosomal markers was shown to be independent of oct transfer, whereas the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on oct plasmid transfer. the presence of a fertility factor termed fpo h ...1979762014
dissociation of the nic plasmid aggregate in pseudomonas putida.the nic plasmid on conjugal transfer from pseudomonas convexa pc 1 to pseudomonas putida ppg 1 dissociates into an independent fertility factor t and a nontransmissible nic structural gene plasmid.1979762028
the amino acid composition of histidine ammonialyase from pseudomonas putida ncib 10807.the amino acid composition of histidine ammonia-lyase from pseudomonas putida ncib 10807 suggests that this enzyme may be different from the pseudomonas testosteroni ncib 10808 histidine ammonia-lyase, whose amino acid composition is known.1979488259
program of bacteriophage gh-1 dna transcription in infected pseudomonas putida.the program of transcription in phage gh-1-infected pseudomonas putida was examined. it was found that the host p. putida rna polymerase transcribes early rna from the l strand of gh-1 dna during the initial stages of infection. the host rna polymerase is also undoubtedly responsible for transcription of complementary rna late in the infectious cycle because complementary rna was not transcribed when rifampin was added to the infected cell culture. the gh-1-induced rna polymerase transcribes lat ...1979480467
a theoretical interpretation of the variations of some physical parameters within the [2fe-2s] ferredoxin group.a model is proposed to explain the variation of some physical parameters within the reduced [2fe-2s] ferredoxin group. according to this model, the main effects result from a variable mixing of some d orbitals of the fe2+ ion owing to rhombic distortion of the active site having the same geometrical character, but different in intensity, for each protein. some peculiar experimental results such as the axial electron paramagnetic resonance spectra of adrenal ferredoxin and pseudomonas putida ferr ...1979465523
nonfermentative bacilli: evaluation of three systems for identification.three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ...1979389945
formaldehyde dehydrogenase from pseudomonas putida. purification and some properties.formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of pseudomonas putida c-83 by chromatographies on columns of deae-cellulose, deae-sephadex a-50, and hydroxyapatite. the purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at ph 7.8 using formaldehyde as a substrate. the enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. the enzyme w ...1979571868
stereochemistry of the conversion of methacrylate to beta-hydroxyisobutyrate in pseudomonas putida. 1979572832
stereospecific hydrogen loss in the conversion of [2h7]isobutyrate to beta-hydroxyisobutyrate in pseudomonas putida. the stereochemistry of beta-hydroxyisobutyrate dehydrogenase. 1979573276
purification of glutamine synthetase from a variety of bacteria.we have developed two procedures which allow the very rapid purification of glutamine synthetase (gs) from a diverse variety of bacteria. the first procedure, based upon differential sedimentation, depends upon the association of gs with deoxyribonucleic acid in cell extracts. the second procedure, derived from the method of c. gross et al (j. bacteriol. 128:382-389, 1976) for purifying ribonucleic acid polymerase by polyethylene glycol (peg) precipitation, enabled us to obtain high yields of gs ...19806102984
the conformation of catalytically functional schiff's bases: the pyruvate--lysine ketimine of 2-keto-3-deoxygluconate-6-phosphate aldolase of pseudomonas putida. 19806109521
substrate-mediated inactivation of urocanase from pseudomonas putida. evidence for an essential sulfhydryl group.incubation of urocanase from pseudomonas putida with either its substrate, urocanic acid, or product, 4'(5')-imidazolone-5'(4')-propionic acid, resulted in an oxygen-dependent inhibition of enzyme activity. coincident with the inactivation was the stoichiometric incorporation of radioactivity from [14c]urocanate into the protein. nad+ which is required for activity or urocanase was not directly involved in the inactivation process. the inactivation of urocanase was irreversible, could be partial ...19806109547
expression of the lactose transposon tn951 in escherichia coli, proteus and pseudomonas.the control of beta-galactosidase specified by the lactose transposon tn951 (inserted into rp1 to give pgc9114) has been studied in escherichia coli k12, proteus mirabilis, pseudomonas aeruginosa and pseudomonas putida; in the first two species comparison could be made with flac. in e. coli k12, the tn951 and chromosomally encoded enzymes showed marked qualitative differences in regulatio, the former giving a substantially lower maximum induced level and induction ratio. several parameters were ...19806251161
[plasmid characteristics of naphthalene and salicylate biodegradation in pseudomonas putida].the object of this work was to study the physico-chemical and biological properties of dnas of the biodegradation plasmids nah and sal. a comparative analysis of the physico-chemical parameters for these dnas made it possible to detect a number of identical properties in them: the same sedimentation profile for covalently-closed circular dna forms, 68--70 s; the molecular weight of ca. 50 md; a roughly equal number of fragments (up to 23) was found when the dnas of nah and sal were restricted by ...19806259498
[effect of humic acids from the brown coal of the aleksandriyskoya field on pseudomonas putida growth]. 19806446021
mutants defective in isomerase and decarboxylase activities of the 4-hydroxyphenylacetic acid meta-cleavage pathway in pseudomonas putida.degradation of 2-hydroxy-5-carboxymethylmuconic semialdehyde, the ring fission product of the 4-hydroxyphenylacetate meta-cleavage pathway, by mutant strains p23x19 and p23x16 of pseudomonas putida nci b 9865 was studied. both mutants were unable to grow on either 4-hydroxyphenylacetate of 3,4-dihydroxyphenylacetate. cell extracts of p23x19, grown in the presence of 3,4-dihydroxyphenylacetate, degraded the ring fission product to a compound that accumulated and had maximum uv absorption at 300 n ...19806769900
reactivation studies on putidamonooxin -- the monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida. 19806772176
headspace analysis of volatile metabolites of pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry.gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of pseudomonas aeruginosa and 1 strain each of pseudomonas cepacia, pseudomonas putida, pseudomonas putrefaciens, pseudomonas fluorescens, and pseudomonas maltophilia. all strains of pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. the other strains failed to produce 2-aminoacetophenone. ...19806775012
expression of e. coli genes carried by hybrids of plasmid rp4.hybrids of the rp4 plasmid, containing bacteriophage mu and chromosomal genes of escherichia coli, were transferred into salmonella typhimurium, pseudomonas putida, pseudomonas aeruginosa and proteus mirabilis. the individual genes of the arginine, histidine, leucine and threonine operons were expressed in these microorganisms.19806777258
inhibition, but not uncoupling, of respiratory energy coupling of three bacterial species by nitrite.the effect of nitrite on respiratory energy coupling of three bacteria was studied in light of a recent report that nitrite acted as an uncoupling agent with paracoccus denitrificans grown under denitrifying conditions. our determinations of proton translocation stoichiometry of pseudomonas putida (aerobically grown), pseudomonas aeruginosa, and p. denitrificans (grown both aerobically and under denitrifying conditions) showed nitrite inhibition of proton-to-oxidant stoichiometry, but not uncoup ...19806777373
comparative immunochemical studies of cytochrome p-450cam of pseudomonas putida and of cytochrome p-450scc of bovine adrenocortical mitochondria. 19806780340
[phage pf16 interrelationships with pseudomonas putida bacteria. i. unstable transductants and mutants of pseudomonas putida pfg1 resistant to phage pf16].the frequencies of transduction of the chromosomal genes by pf16 in pseudomonas putida pgg1 are dependent on the marker transduced and unpredictable. histidine and isoleucine-valine positive transductants, which are resistant to pf16, have been selected in the crosses with low (about 10(-8) for phage units) frequencies of transduction. some of these transductants carry new mutations. the transductants are unstable for phage resistance and histidine or isoleucine-valine positive characters. there ...19806928126
construction of a partial diploid for the degradative pathway encoded by the tol plasmid (pwwo) from pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyir gene. 19806929031
primary structure of serratia marcescens anthranilate synthase component ii.the amino acid sequence of anthranilate synthase component ii (as ii) from serratia marcescens was determined. the cysteine residue essential for glutamine utilization was alkylated selectively by iodo [1-14c]acetamide prior to separation of the two protein components of anthranilate synthase. the isolated as ii then was subjected to cleavage by cyanogen bromide and by trypsin after citraconylation to obtain overlapping fragments. as ii is a single polypeptide chain of 192 residues having a calc ...19806986371
regulation of enzymes of the 3,5-xylenol-degradative pathway in pseudomonas putida: evidence for a plasmid.constitutive synthesis of enzymes responsible for methyl group oxidation in 3,5-xylenol degradation and an associated p-cresol methylhydroxylase in pseudomonas putida ncib 9869 was shown by their retention at high specific activities in cells transferred from 3,5-xylenol medium to glutamate medium. the specific activities of other enzymes of the 3,5-xylenol pathway declined upon removal of aromatic substrate, consistent with their inducible control. specific activities of the methyl-oxidizing en ...19806989805
[nucleoside catabolism study of pseudomonas putida and pseudomonas trifolii].no activity of specific nucleoside phosphorylases was detected in pseudomonas putida; as the result, its cells cannot grow on various nucleosides using them as carbon sources. insignificant growth on ribonucleosides, viz. inosine and uridine, should be attributed to the activity of hydrolases in the cell. in contrast, pseudomonas trifolii is capable of normal growth on various nucleosides using them as a sole source of carbon. accordingly, the cell extracts display the activities of enzymes invo ...19806993879
effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography.high-resolution pyrolysis gas-liquid chromatography was applied to three bacteria (escherichia coli nctc 9001, pseudomonas putida (ncib 9494, and staphylococcus aureus nctc 8532) grown under a variety of conditions. changing the culture medium drastically altered the quantitative aspects of the pyrograms of all three organisms, but the effects of culture time and incubation temperature were less severe. mathematical analysis of the relative peak heights showed that four peaks could be used to di ...19806999989
chemical structure and inhalation toxicity of lipopolysaccharides from bacteria on cotton.lipopolysaccharides from different bacteria isolated from cotton were purified and chemically analyzed. their pulmonary toxicity to animals was tested in inhalation tests. lipopolysaccharides from agrobacterium and xanthomonas were shown to differ from the others in that they contained no heptose and no non-hydroxylated fatty acids with a chain length of 12, 14, or 16 carbon atoms. lipopolysaccharides from pseudomonas putida, enterobacter agglomerans, and klebsiella oxytoca were found to cause a ...19807000706
naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase.1,2-dihydroxynaphthalene oxygenase was purified from pseudomonas putida ncib 9816 grown on naphthalene as the sole source of carbon and energy. the enzyme had a subunit molecular weight of 19,000 and in a medium containing phosphate buffer, 1 mm mercaptoethanol, and 10% (vol/vol) ethanol had a native molecular weight greater than 275,000. the enzyme required fe2+ for activity. it was inactivated slowly on standing, and inactivation was accelerated by dilution with aerated buffers and by h2o2. ba ...19807204331
p-cymene pathway in pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs.several classes of mutants of pseudomonas putida (jt810) defective in the utilization of p-cymene as sole carbon source have been isolated. selective enrichment of the mutants and for strains putatively cured of a degradative plasmid was achieved by incubation of cells in minimal growth media containing p-cymene (or p-cumate) and various halogenated analogs of the growth substrates or pathway intermediates. analogs which led to successful enrichments included: p-chlorotoluene, p-bromotoluene, al ...19807204334
biological distribution and physiological role of the beta-ketoadipate transport system.beta-ketoadipate induces catabolic enzymes in pseudomonas putida. the compound is transported by a system which also concentrates adipate, a non-metabolizable analogue of beta-ketoadipate. the natural substrate, beta-ketoadipate, competitively inhibits adipate transport with a k1 of 0.04 mm, lower than the km of 0.23 mm observed with adipate. transport is inhibited competitively by succinate (k1 1.3 mm) and non-competitively by acetate (k1 5.3 mm). the system has a sharp ph optimum at 5.5. trans ...19807217919
d-glucose and d-gluconate transport in vesicles from pseudomonas putida.vesicles prepared from glucose-grown cells of pseudomonas putida (atcc, 12633) retain glucose oxidase (gox) and gluconate dehydrogenase (gadh) activity and actively transport d-glucose, 2-deoxy-d-glucose (2dog), 3-deoxy-3-fluoro-d-glucose (3fg), and d-gluconate by saturable processes. the transport of these substrates is stimulated by the addition of l-malate or reduced phenazine methosulphate (pms). vesicles prepared from succinate-grown cells of p. putida lose their capacity to transport d-glu ...19807248836
digestion of algin by pseudomonas maltophilia and pseudomonas putida.pseudomonas maltophilia and pseudomonas putida were identified as alginolytic species. two media used for demonstrating alginolytic activity are described. the applied aspects of the ability of these two species to digest algin are discussed.19807356324
the purification and characterization of delta 5-3-ketosteroid isomerase from pseudomonas putida, a cysteine-containing isomerase.a delta 5-3-ketosteroid isomerase (ec 5.3.3.1) has been isolated from pseudomonas putida biotype b and purified to homogeneity. this previously undescribed steroid isomerase resembles that isolated from pseudomonas testosteroni (talalay, p., and wang, v.s. (1955) biochim. biophys. acta 18, 300-301). the enzyme is induced by various steroids, has a subunit molecular weight of 13,750 +/- 250, a pi of 4.8 +/- 0.1 has a specific activity of 40,000 units/mg, using 5-androstene-3,17-dione as the subst ...19807358699
kinetics of the isomerization of 5-androsten-3,17-dione catalyzed by delta 5-3-ketosteroid isomerase from pseudomonas putida. 19807358700
active site-directed photoinactivation of delta 5-3-ketosteroid isomerase from pseudomonas putida dependent on 1,4,6-androstatrien-3-one-17 beta-ol.delta 5-3-ketosteroid isomerase from pseudomonas putida is subject to photoinactivation by light of wavelengths greater than 300 nm, specifically in the presence of the competitive inhibitor, 1,4,6-androstatrien-3-one-17 beta-ol (teo). in the absence of this steroid or in the presence of the nonchromophoric steroidal competitive inhibitor, deoxycholate, the enzyme activity is essentially unaffected by irradiation. deoxycholate protects the enzyme from the teo-dependent reaction to a degree which ...19807358701
purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1.the benzoate 1,2-dioxygenase system of pseudomonas arvilla consists of two proteins, an nadh-cytochrome c reductase and an oxygenase. the oxygenase component was purified to apparent homogeneity by the criteria of polyacrylamide gel electrophoresis from benzoate-induced cells of p. arvilla. the molecular weight of the enzyme was determined to be 273,000 by sedimentation equilibrium analysis, 280,000 by electrophoresis on polyacrylamide gels of different concentrations, and 270,000 by sepharose c ...19807372624
energetics of myo-inositol transport in pseudomonas putida.the effects of specific inhibitors on the high-affinity transport system of cyclitols and on the respiration of pseudomonas putida shows that the transport activity is dependent on high energy phosphate bond.19807379940
hybrid pathway for chlorobenzoate metabolism in pseudomonas sp. b13 derivatives.derivatives of pseudomonas sp. b13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the tol plasmid of pseudomonas putida mt-2 were studied. the utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. during growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deo ...19807380800
production of methanol from aromatic acids by pseudomonas putida.when grown at the expense of 3,4,5-trimethoxybenzoic acid, a strain of pseudomonas putida oxidized this compound and also 3,5-dimethoxy-4-hydroxybenzoic (syringic) and 3,4-dihydroxy-5-methoxybenzoic (3-o-methylgallic) acids; but other hydroxy- or methoxy-benzoic acids were oxidized slowly or not at all. radioactivity appeared exclusively in carbon dioxide when cells were incubated with [4-methoxyl-14c]trimethoxybenzoic acid, but was found mainly in methanol when[methoxyl-14c]3-o-methylgallic aci ...19807380811
a simple method for the preparation of d-alpha-aminoadipic acid.high quantity (1 g and more) of racemically and chromatographically pure d-alpha-aminoadipic acid was prepared by selective metabolism of the l-isomer of the commercially available dl-alpha-aminoadipate by pseudomonas putida. the overall yield of this preparation averaged 40%. the final product has [a]25d value of -25 degrees. this procedure can be useful in the synthesis of high purity d-alpha-amino-adipate, a compound shown recently to be a useful tool in the study of neurotransmission mechani ...19807383979
repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida.muconolactone delta-isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ...19807391022
homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases.gamma-carboxymuconolactone decarobxylase (ec 4.1.1.44) and muconolactone isomerase (ec 5.3.3.4) mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ...19807391024
[microbiological transformation of quinoline by pseudomonas putida bacteria]. 19807402102
[electrokinetic properties of arthobacter siderocapsulatus].the electro-kinetic properties of arthrobacter siderocapsulatus were determined using microelectrophoresis in alternating electric field. the surface of bacterial cells was shown to bear positive charge within a wide range of ph. the positive charge of the cells should be attributed apparently to the presence of mucous capsules containing manganese dioxide since the growth of the bacterium is known to involve oxidation of manganese or ferrous iron and accumulation of their hydroxides in the caps ...19807402120
degradation of (-)-ephedrine by pseudomonas putida. detection of (-)-ephedrine: nad+-oxidoreductase from arthrobacter globiformis.a bacterium utilizing the alkaloid (-)-ephedrine as its sole source of carbon was isolated by an enrichment-culture technique from soil supplemented with 4-benzoyl-1,3-oxazolidinon-(2). the bacterium was indentified as pseudomonas putida by morphological and physiological studies. the following metabolites were isolated from the culture fluid: methylamine, formaldehyde, methylbenzoylcarbinol (2-hydroxy-1-oxo-1 phenylpropane), benzoid acid, pyrocatechol and cis, cis-muconic acid. a pathway for th ...19807405363
cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from pseudomonas ovalis.the cd-, cr-, and mn-substituted enzymes of iron-superoxide dismutase from pseudomonas ovalis were prepared from the apoenzyme by using the alkaline treatment method described before (1) with a slight modification. the cd-substituted enzyme had 1.16 g atoms of cd per mol of enzyme and no visible absorption. the cr-substituted enzyme had 1.27 g atoms of cr per mol of enzyme and had absorption maxima at 530 nm and 670 nm with a shoulder around 370 nm. the mn-substituted enzyme had 1.27 g atoms of ...19807410333
enzymological aspects of caffeine demethylation and formaldehyde oxidation by pseudomonas putida c1.1) the enzymatic demethylation of caffeine (1,3,7-trimethylxanthine) by pseudomonas putida c1 was investigated; an inducible enzyme system has been observed. this enzyme shows an optimum ph of about 6.0, and the optimum temperature is in the range of 22-24 degrees c. the enzyme is absolutely dependent on nadh or nadph as a cosubstrate and is activated by co2+. 2) the formaldehyde generated by the demethylation of caffeine is oxidized by an nad-dependent formaldehyde dehydrogenase, which is indep ...19807461603
biological process for converting naphthalene to cis-1,2-dihydroxy-1,2-dihydronaphthalene.a biological process for converting naphthalene to cis-1,2-dihydroxy-1,2-dihydronaphthalene (dhd) catalyzed by pseudomonas putida strain 119 was optimized in flask experiments. these studies revealed the following: (i) p. putida 119 can propagate efficiently and produce dhd when supplied one of several carbon sources and naphthalene; (ii) maximum dhd production by p. putida 119 occurs in logarithmic-growth-phase cells and decreases at various rates in the stationary growth phase, depending upon ...198016345504
separation of d-(+)-nicotine from a racemic mixture by stereospecific degradation of the l-(-) isomer with pseudomonas putida.incubation of racemic mixtures of dl-(+/-)-nicotine with pseudomonas putida resulted in a complete stereoselective degradation of the l-(-) isomer. unnatural d-(+)-nicotine, which is of pharmacological interest for stereochemical studies of various nicotine-responsive systems, was not affected by the bacterium and was recovered by extraction.198016345568
symbiotic utilization of polyvinyl alcohol by mixed cultures.polyvinyl alcohol (pva)-utilizing cultures were obtained from various sources. they were mixed cultures even after cyclical transfer to liquid and plate media with pva as a sole source of carbon. component bacteria were isolated from the several mixed cultures, and it was shown that pva was utilized symbiotically by two bacterial members which could not utilize pva in each respective pure culture. from a mixed culture, strains vm15, vm15a (pseudomonas putida) and vm15c (pseudomonas sp.) were iso ...198116345693
[pseudomonas putida ppg1 resistance to different bacteriophages].cris-cross resistance analysis has been accomplished for some pseudomonas putida ppg1 mutants resistant to pf16, af, tf and pmw phages. on the basis of the results, a formal scheme of the receptor sites for these phages on the bacterial cell surface was drawn. study of the sensitivity of p. putida ppg1 phage-resistant mutants an unexpected phenomenon. some of the phages specific to p. putida b-21 and b-31 which did not adsorb on the ppg1 strain acquired the ability on certain types of the ppg1 p ...19817198071
control of microorganisms in the rhizosphere of wheat by inoculation of seeds with pseudomonas putida and by foliar application of urea.after inoculation of wheat seeds with various bacterial strains germination of plants was usually inhibited at first but growth was stimulated later. after inoculation with pseudomonas putida k 11 producing physiologically active compounds the total number of bacteria increased together with the bacteria: fungi ratio in the rhizosphere. these characteristic were further increased after foliar application of urea due to increased root exudation. dry mass of upper wheat parts was about 14--80% hig ...19817203287
inhibition of catechol 2,3-dioxygenase from pseudomonas putida by 3-chlorocatechol.partially purified preparations of catechol 2,3-dioxygenase from toluene-grown cells of pseudomonas putida catalyzed the stoichiometric oxidation of 3-methylcatechol to 2-hydroxy-6-oxohepta-2,4-dienoate. other substrates oxidized by the enzyme preparation were catechol, 4-methylcatechol, and 4-fluorocatechol. the apparent michaelis constants for 3-methylcatechol and catechol were 10.6 and 22.0 mum, respectively. substitution at the 4-position decreases the affinity and activity of the enzyme for ...19817259155
bacterial degradation of 3,4,5-trimethoxycinnamic acid with production of methanol.when grown on 3,4,5-trimethoxycinnamic acid, a strain of pseudomonas putida oxidized this compound and also 3,4,5-trimethoxybenzoic, 3,5-dimethoxy-4-hydroxybenzoic (syringic), and 3,4-dihydroxy-5-methoxybenzoic (3-o-methylgallic) acids, but 3,5-dimethoxy-4-hydroxycinnamic and other acids bearing structural resemblances to the growth substrate were oxidized only slowly. these results indicate that two carbon atoms of the side chain of 3,4,5-trimethoxycinnamate were released before oxidative demet ...19817263612
plasmid- and chromosome-mediated dissimilation of naphthalene and salicylate in pseudomonas putida pmd-1.pseudomonas putida pmd-1 dissimilates naphthalene (nah), salicylate (sal), and benzoate (ben) via catechol which is metabolized through the meta (or alpha-keto acid) pathway. the ability to utilize salicylate but not naphthalene was transferred from p. putida pmd-1 to several pseudomonas species. agarose gel electrophoresis of deoxyribonucleic acid (dna) from pmd-1 and sal+ exconjugants indicated that a plasmid (pmwd-1) of 110 megadaltons is correlated with the sal+ phenotype; restriction enzyme ...19817275935
metabolism of allylglycine and cis-crotylglycine by pseudomonas putida (arvilla) mt-2 harboring a tol plasmid.spontaneous mutants which acquired the ability to utilize d-allylglycine (d-2-amino-4-pentenoic acid) and dl-cis-crotylglycine (dl-2-amino-cis-4-hexenoic acid) but not l-allylglycine or dl-trans-crotylglycine could be readily isolated from pseudomonas putida mt-2 (pam1). derivative strains of pam1 putatively cured of the tol (pwwo) plasmid were incapable of forming mutants able to utilize the amino acids for growth; however, this ability could be regained by conjugative transfer of the tol (pwwo ...19817287632
regulation of phenol degradation in pseudomonas putida.in order to characterize the ability of pseudomonas putida (trevisan 1889) migula 1895 strain h to degrade various mono- and diphenolic aromatic compounds, respiratory activities towards phenol, catechol, and the cresol isomers were determined. the following rates of oxygen uptake (qo2) were obtained with resting phenol-grown cells: phenol -- 229, o-cresol -- 231, m-cresol -- 43, p-cresol -- 200, catechol -- 262. all these compounds were oxidized by a two-phase-kinetics, the first phase is chara ...19817293241
magnetic and natural circular dichroism spectra of cytochgromes p-450(11) beta and p-450scc purified from bovine adrenal cortex.magnetic (mcd) and natural circular dichroism (cd) spectra various complexes of cytochrome p-450(11) beta (p-450(11) beta) and cytochrome p-450scc (p-450scc) from bovine adrenal cortex were measured from 250 nm to 700 nm. mcd and cd spectral contours of cytochromes p-450(11) beta and p-450scc in the soret and visible regions were, as a whole, analogous to those of cytochromes p-450 from rabbit liver microsomes and also from pseudomonas putida in their high-spin ferric, high-spin ferrous and ferr ...19817295771
purification of a branched-chain keto acid dehydrogenase from pseudomonas putida.we purified branched-chain keto acid dehydrogenase to a specific activity of 10 mumol/min per mg of protein from pseudomonas putida grown on valine. the purified enzyme was active with 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate in a ratio of 1.0:0.8:0.7 but showed no activity with either pyruvate or 2-ketoglutarate. there were four polypeptides in the purified enzyme (molecular weights, 49,000, 46,000, 39,000, and 37,000). the purified enzyme was deficient in the specific ...19817298579
2,3-dihydroxybenzoate pathway in pseudomonas putida. 1h n.m.r. study on the ring-cleavage site.1. ring cleavage of 2,3-dihydroxybenzoate by cell-free extracts of pseudomonas putida leads to 2-hydroxy-6-oxo-(2z,4e)-hexa-2,4-dienoic acid and co2. 2. the 1h n.m.r. spectrum of the ring-fission product obtained in a 2h2o solution suggests that the extra-diol cleavage occurs between c-3 and c-4.19817306005
mössbauer spectroscopic studies of the terminal dioxygenase protein of benzene dioxygenase from pseudomonas putida.mössbauer spectra obtained from the terminal dioxygenase protein of the benzene dioxygenase system from pseudomonas putida show that it contains [2fe--2s] centres similar to those of the two-iron plant-type ferredoxins. in the oxidized form the two iron atoms within the centre are high-spin ferric but with considerable inequivalence. in the reduced form the centre contains one extra electron, and this is localized on one of the iron atoms, which becomes high-spin ferrous.19817306045
the reaction of oxygen with protocatechuate 3,4-dioxygenase from pseudomonas putida. characterization of a new oxygenated intermediate.the reactions of protocatechuate dioxygenase (protocatechuate:oxygen 3,4-oxidoreductase, ec 1.13.11.3) with substrates and oxygen have been studied at 4 degrees c using rapid kinetic techniques. in this study, two oxygenated intermediates were kinetically and spectrally characterized. the rate of oxygen addition to the enzyme-substrate complex was determined to be 5 x 10(5) m-1 s-1. this oxygenated complex rapidly converts (450 s-1) to another spectrally identifiable compound which then breaks d ...19817309730
participation of the beta-ketoadipate transport system in chemotaxis.beta-ketoadipate serves as a chemoattractant for pseudomonas putida. the chemotactic response is inducible, and a regulatory mutant strain that forms the beta-ketoadipate transport system at high levels exhibits a heightened chemotactic response to beta-ketoadipate. adipate and succinate, compounds that interact with the transport system, inhibit chemotaxis toward beta-ketoadipate. some, but not all, mutants that fail to respond chemotactically to beta-ketoadipate lack the beta-ketoadipate trans ...19817320700
[evaluation of biological activity of l-methionidase from pseudomonas putida ac-75 on the basis of in vitro studies]. 19817342702
[arsenic oxidation by the heterotrophic bacteria pseudomonas putida and alcaligenes eutrophus].two heterotrophic bacteria, pseudomonas putida 18 and alcaligenes eutrophus 280, were isolated from gold-arsenic deposits. the bacteria oxidize as(iii) to as(v) at ph 6-9 and temperatures 4-28 and 28 degrees c respectively. the oxidation is accompanied by a decrease in the ph of the medium. the rate of the oxidation directly depends on the number of cells in the inoculum.19817219219
[the identification of nonfermentative gram-negative bacteria. experiences with 676 apyocyaninogenic strains (author's transl)].during a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. of the, 1205 (69%) were p. aeruginosa, 124 (10%) of which failed to produce pyocyanin. the apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. for identification of species two or three steps were needed. by this procedure, 530 of the 552 strains could be assigned to nineteen species wi ...19817223132
isolation and characterization of spontaneously occurring tol plasmid mutants of pseudomonas putida hs1.a strain of pseudomonas (p. putida hs1) was found to resemble p. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. the ability to degrade these substrates by p. putida hs1 (ppc1) was shown to be encoded by a tol (pdk1) plasmid as evidenced by: (i) spontaneous loss of the tol-related phenotype after growth with benzoate, (ii) transfer o ...19817240090
interaction of 5-bromocamphor with cytochrome p-450 cam. production of 5-ketocamphor from a mixed spin state hemoprotein.camphor is stereospecifically hydroxylated by the soil bacterium pseudomonas putida at the 5-exo position by a cytochrome p-450 mixed function oxidase system consisting of a flavoprotein reductase; putidaredoxin, an iron-sulfur oxidation-reduction transport-effector protein; and the p-450 hemoprotein. we have studied the interaction of a substrate analog of camphor, 5-exo-bromocamphor, with this cytochrome p-450 mixed function oxidase system in order to probe the molecular mechanisms of electron ...19817240237
reconstitution of iron-sulfur cluster of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1.nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, is an ion-sulfur flavoprotein with one fad and one iron-sulfur cluster of [2fe-2s] type (yamaguchi, m., and fujisawa, h. (1978) j. biol. chem. 253, 8848-8853). treatment of nadh-cytochrome c reductase with p-chloromercuriphenylsulfonic acid resulted in fading of its color with a concomitant loss of the nadh-cytochrome c reductase activity. the p-chloromercuriphenylsulfonic acid-treated enzyme was found to contain one fa ...19817240244
8 alpha-(o-tyrosyl)flavin adenine dinucleotide, the prosthetic group of bacterial p-cresol methylhydroxylase.8 alpha-(o-tyrosyl)riboflavin has been synthesized by condensation of the copper complex of l-tyrosine with 8 alpha-bromotetraacetylriboflavin. the structure of this synthetic product was proven by absorption and 1h nmr spectroscopy and by chemical degradation, which yielded 1 mol of tyrosine per mol of flavin. the synthetic compound comigrated wtih the (aminoacyl)riboflavin isolated from the p-cresol methylhydroxylase of pseudomonas putida, and both showed identical absorption and fluorescence ...19817248267
purification and properties of nadh-ferredoxintol reductase. a component of toluene dioxygenase from pseudomonas putida.cells of pseudomonas putida, after growth with toluene, contain a multicomponent enzyme system that oxidizes toluene to (+)-1(s),2(r)-dihydroxy-3-methyl-cyclohexa-3,5-diene. one of these components has been purified to homogeneity and shown to be a flavoprotein that contains fad as the only detectable prosthetic group. fad was removed from the enzyme during purification. however, equilibrium dialysis experiments showed that the enzyme can bind one mol of fad/mol of enzyme protein. the apparent m ...19817204373
mechanism of salicylate hydroxylase-catalyzed decarboxylation.salicylate hydroxylase (salicylate, nadh: oxygen oxidoreductase (1-hydroxylating, decarboxylating), ec 1.14.13.1) in pseudomonas putida catalyzed hydroxylation of the substrate analogue, salicylaldehyde, to form catechol and formate with stoichiometric consumption of nadh and o2. consequently, a study of primary product derived from the carboxyl group of the authentic substrate, salicylate, was undertaken. the experimental results revealed that co2 not h2co3, was produced first.19817213760
catabolism of pseudocumene and 3-ethyltoluene by pseudomonas putida (arvilla) mt-2: evidence for new functions of the tol (pwwo) plasmid.pseudocumene (1,2,4-trimethylbenzene) and 3-ethyltoluene were found to serve as growth substrates for pseudomonas putida (arvilla) mt-2, in addition to toluene, m-xylene, and p-xylene as previously described. similar observations were made with several additional p. putida strains also capable of growth with toluene and the xylenes. additional substrates which supported the growth of these organisms included 3,4-dimethylbenzyl alcohol, 3,4-dimethylbenzoate, and 3-ethylbenzoate. p. putida mt-2 ce ...19817216999
plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway.a plasmid, termed pac25, specifying biodegradation of 3-chlorobenzoate in a strain of pseudomonas putida has been characterized. during growth of the plasmid-harboring cells with 3-chlorobenzoate, there was an accumulation of 3-chlorocatechol and beta-chloromuconic acid as intermediates and release of more than 80% of the chlorine in the form of inorganic chloride. the plasmid had a mean molecular mass of 68 x 10(6) daltons and was transmissible to a number of pseudomonas species such as p. aeru ...19817217013
inability of the api-20e system to speciate the fluorescent group of pseudomonads.one thousand randomly selected clinical isolates of the fluorescent group of pseudomonas were speciated in parallel by the api-20e system and by a classic microbiologic 17-test battery. the classic battery identified the isolates as 993 pseudomonas aeruginosa, five pseudomonas putida, and two pseudomonas fluorescens. to augment the p. putida and p. fluorescens data, 52 reference isolates were also tested in parallel. api-20e was found to identify 99% of p. aeruginosa as p. aeruginosa. however, t ...19817008576
molecular cloning of tol genes xylb and xyle in escherichia coli.the xylb and xyle genes in the tol plasmid of pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pbr322 in escherichia coli for detailed mapping. the xylb gene was mapped in a 2.9-kilobase region within the bamhi bc fragment of ptn2, an in vivo rp4-tol recombinant, whereas the xyle gene was mapped in a 1.8-kilobase region within the bamhi bd fragment. the directions of transcription of these genes were deduced f ...19817009570
incorporation of substrate cell lipid a components into the lipopolysaccharide of intraperiplasmically grown bdellovibrio bacteriovorus.the composition of bdellovibrio bacteriovorus lipopolysaccharide (lps) was determined for cells grown axenically and intraperiplasmically on escherichia coli or pseudomonas putida. the lps of axenically grown bdellovibrios contained glucose and fucosamine as the only detectable neutral sugar and amino sugar, and nonadecenoic acid (19:1) as the predominant fatty acid. additional fatty acids, heptose, ketodeoxyoctoic acid, and phosphate were also detected. lps from bdellovibrios grown intraperipla ...19817024249
excision of the 40kb segment of the tol plasmid from pseudomonas putida mt-2 involves direct repeats. 19816950198
molecular and functional analysis of the tol plasmid pwwo from pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.the genetic organization of the pseudomonas putida plasmid pwwo-161, which encodes enzymes for the degradation of toluene and related aromatic hydrocarbons, has been investigated by transposition mutagenesis and gene cloning. catabolic genes were localized to two clusters, one for upper pathway (hydrocarbon leads to carboxylic acid) enzymes and the other for lower pathway (carboxylic acid leads to tricarboxylic acid cycle) enzymes, that are separated by a 14-kilobase dna segment. the physical or ...19816950388
isolation of two lipoamide dehydrogenases from pseudomonas putida grown on valine. 19816895028
control of meta-cleavage degradation of 4-hydroxyphenylacetate in pseudomonas putida.synthesis of enzymes of the 4-hydroxyphenylacetate meta-cleavage pathway was studied in pseudomonas putida wild-type strain p23x1 (ncib 9865) and mutant strains which had either structural or regulatory gene mutations. induction studies with mutant strains each defective in an enzyme of the pathway showed that 4-hydroxyphenylacetate induced the hydroxylase and that 3,4-dihydroxyphenylacetate induced the 2,3-oxygenase, aldehyde dehydrogenase, isomerase, decarboxylase, and hydratase. this showed t ...19816895079
isolation of a specific lipoamide dehydrogenase for a branched-chain keto acid dehydrogenase from pseudomonas putida.we purified lipoamide dehydrogenase from cells of pseudomonas putida ppg2 grown on glucose (lpd-glu) and lipoamide dehydrogenase from cells grown on valine (lpd-val), which contained branched-chain keto acid dehydrogenase. lpd-glu had a molecular weight of 56,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and lpd-val had a molecular weight of 49,000. the ph optimum for lpd-glu for reduced nicotinamide adenine dinucleotide oxidation was 7.4, compared with ph 6.5 f ...19816895373
isolation of alginate-producing mutants of pseudomonas fluorescens, pseudomonas putida and pseudomonas mendocina.spontaneous alginate-producing (muc) variants were isolated from strains of pseudomonas fluorescens, p. putida and p. mendocina at a frequency of 1 in 10(8) by selecting for carbenicillin resistance. the infrared spectrum of the bacterial exopolysaccharide was typical of an acetylated alginate similar to that previously described in azotobacter vinelandii and in mucoid variants of p. aeruginosa. mucoid variants were not isolated from p. stutzeri, p. pseudoalcaligenes, p. testosteroni, p. diminut ...19816801192
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