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exchange reactions catalyzed by methioninase from pseudomonas putida. isolation and characterization of the exchange products.gel-electrophoretically homogeneous methioninase l-methionine methanethiol-lyase (deaminating), ec 4.4.1.11 of pseudomonas putida, which catalyzes alpha, beta- and alpha, gamma-eliminations from s-substituted amino acids, could also catalyze a variety of beta- and gamma-exchange reactions, according to the following equations: rsch2ch(nh2)cooh+r'sh in equilibrium r'sch2ch(nh2)cooh+rsh (beta-exchange) and rsch2ch2ch(nh2)cooh+r'sh in equilibrium r'sch2ch2ch(nh2)cooh+rsh (gamma-exchange), where r's ...197614124
bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from pseudomonas putida orc.the hydroxylase activities observed in extracts of pseudomonas putida orc after growth on orcinol and resorcinol as sole source of carbon have been purified to homogeneity. both enzymes were shown to be flavoproteins and to contain approximately 1 mol of fad for each polypeptide chain, s20,w values for each enzyme are 4.1 +/- 0.1 and are independent of the presence of their aromatic substrates. molecular weight determinations under native (approximately 68000) and denaturing (approximately 70000 ...19761280
crystalline aspartate aminotransferase from pseudomonas striata. 19761290
cytochrome p450cam and its complexes. mössbauer parameters of the heme iron.mössbauer spectroscopy has been used to study the heme iron in various states of cytochrome p450cam from the camphor-hydroxylating system of the bacterium pseudomonas putida. native, camphor-free p450cam contains low-spin ferric iron, part of which (approx. 50-70%) is converted to the high-spin ferric state upon addition of camphor. the mössbauer spectra of the camphor-free enzyme (s equals 1/2) and of the high-spin component (s equals 5/2) of the camphor complex have been successfully simulated ...19762296
properties of anthranilate synthetase component ii from pseudomonas putida.the interaction of pseudomanas putida anthranilate synthetase component ii (as ii) with glutamine, glutamine analogs, and iodoacetamide has been investigated in order to clarify the initial steps in the mechanism for glutamine utilization. as ii is alkylated and irreversibly inactivated by covalent attachment of approximately 1 eg of l-2-amino-4-oxo-5-chloropentanoic acid (chloroketone) or 1 eq of iodoacetamide. alkylation of as ii by chloroketone involves initial formation of an enzyme-inhibito ...1976765343
pathways of d-fructose catabolism in species of pseudomonas.cell-free extracts of d-fructose grown cells of pseudomonas putida, p. fluorescens, p. aeruginosa, p. stutzeri, p. mendocina, p. acidovorans and p. maltophila catalyzed a p-enolpyruvate-dependent phosphorylation of d-fructose and contained 1-p-fructokinase activity suggesting that in these species fructose-1-p and fructose-1,6-p2 were intermediates of d-fructose catabolism. neither the 1-p-fructokinase nor the activity catalyzing a p-enolpyruvate-dependent phosphorylation of d-fructose was prese ...1977139135
cometabolism of products of 1,1,1-trichloro-2,2-bis (p-chlorophenyl)ethane (ddt) by pseudomonas putida. 1977560402
the metabolism of caffeine by a pseudomonas putida strain.1) a bacterium capable of growing aerobically with caffeine (1,3,7-trimethylxanthine) as sole source of carbon and nitrogen was isolated from soil. the morphological and physiological characteristics of the bacterium were examined. the organism was identified as a strain of pseudomonas putida and is referred to as pseudomonas putida c1. 15 additional caffeine-degrading bacteria were isolated, and all of them were also identified as pseudomonas putida strains. the properties of the isolates are d ...1977561017
purification and properties of cis-toluene dihydrodiol dehydrogenase from pseudomonas putida.the purification of (+)-cis-1(s),2(r)-dihydroxy-3-methylcyclohexa-3,5-diene dehydrogenase from cells of pseudomonas putida grown with toluene as the sole source of carbon and energy is reported. the molecular weight of the enzyme is 104,000 at ph 9.7. the enzyme is composed of four apparently identical subunits with molecular weights of 27,000. the enzyme is specific for nicotinamide adenine dinucleotide and oxidizes a number of cis-dihydrodiols. both enantiomers of a racemic mixture of cis-1,2- ...197716865
properties of 1-phosphofructokinase from pseudomonas putida.the 1-phosphofructokinase (1-pfk, ec 2.7.1.56) from pseudomonas putida was partially purified by a combination of (nh4)2so4 fractionation and deae-sephadex column chromatography. in its kinetic properties, this enzyme resembled the 1-pfk's from other bacteria. with the substrates fructose-1-phosphate (f-1-p) and adenosine triphosphate (atp) michaelis-menten kinetics were observed, the km for one substrate being unaffected by a variation in the concentration of the other substrate. at ph 8.0, the ...197717464
the purification and properties of 4-hydroxyisophthalate hydroxylase from pseudomonas putida ncib 9866. 197718349
presence of tightly bound nad+ in urocanase of pseudomonas putida. 197718470
comparison of two dioxygenases from pseudomonas putida.catechol 2,3-dioxygenase and homoprotocatechuate 2,3-dioxygenase were purified from the same strain of pseudomonas putida. molecular weights and subunit sizes were similar, but amino acid compositions showed some marked differences.197719416
photoactivation of urocanase in pseudomonas putida. temperature-compensated in vitro model of an hourglass timer. 197720925
kinetic studies on a 4-methoxybenzoate o-demethylase from pseudomonas putida.a direct, sensitive and reliable photometric assay procedure for monitoring the activity of non-specific 4-methoxybenzoate o-demethylases of microorganisms is described. the assay is based on the o-demethylation of 3-nitro-4-methoxybenzoate to the yellow-coloured product 3-nitro-4-hydroxybenzoate. using this assay and by monitoring the oxidation rate of reduced pyridine nucleotides, the kinetic properties of a purified, reconstituted enzyme system composed of 4-methoxybenzoate monooxygenase (o-d ...1977188654
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...1977329101
[pseudomonas putida: identification, antibiotic sensitivity and pathogenicity (author's transl)].this work studies 51 strains of pseudomonas putida, isolated from clinical specimens (17) and hospital environment (34). identification is performed by study of 41 physiologica and biochemical characters and 78 nutritional characters. according to the two biotypes a and b, described by stanier, palleroni and doudoroff, these 51 strains can be grouped as follows: 48 have typical characters of biotype a, widely predominant, 3 can be distinguished from biotype a only by their auxanogram and include ...1977341053
mercury and organomercurial resistances determined by plasmids in pseudomonas.mercury and organomercurial resistance determined by genes on ten pseudomonas aeruginosa plasmids and one pseudomonas putida plasmid have been studied with regard to the range of substrates and the range of inducers. the plasmidless strains were sensitive to growth inhibition by hg(2+) and did not volatilize hg(0) from hg(2+). a strain with plasmid rp1 (which does not confer resistance to hg(2+)) similarly did not volatilize mercury. all 10 plasmids determine mercury resistance by way of an indu ...1977410779
fractionation of inducible alkane hydroxylase activity in pseudomonas putida and characterization of hydroxylase-negative plasmid mutations.the plasmid-determined inducible alkane hydroxylase of pseudomonas putida resolved into particulate and soluble fractions. spinach reductase and spinach ferredoxin could replace the soluble hydroxylase component. two alkane hydroxylase mutants show in vitro complementation (s. benson and j. shapiro, j. bacteriol., 123: 759-760, 1975): one, alk-7, lacks an active soluble component and the other, alk-181, lacks an active particulate component. together with previous results on a particulate alcoho ...1977410794
regulatory mutations of the pseudomonas plasmid alk regulon.pseudomonas putida strains with plasmids carrying pleiotropic alk mutations gave rise to alkane-positive "revertants," which differ from wild type. some had restricted ability to utilize alkane and primary alcohol growth substrates, and others could grow on undecane and dodecanol, which are not utilized by alk+ strains. these revertants showed altered responses to normal inducers of alka+, alkb+, and alkc+ activities. some revertants were constitutive for these activities. constitutive mutants c ...1977410795
isolation of a mutant tol plasmid with increased activity and transmissibility from pseudomonas putida (arvilla) mt-2.strains with greater ability to dissimilate m-toluate were obtained from the wild-type pseudomonas putida (arvilla) mt-2 that harbors the tol plasmid. increased growth of a mutant strain on aromatic substrates was coupled with simultaneous increase in the activity of metapyrocatechase, an enzyme coded by the tol plasmid, without changing its catalytic properties. in the mutant and the wild-type strains, the inducer specificity and the induction kinetics of metapyrocatechase synthesis were the sa ...1977830645
properties of l-methionine gamma-lyase from pseudomonas ovalis.the distribution of bacterial l-methionine gamma-lyase (l-methionine methanethiollyase (deaminating) (ec 4.4.1.11) was investigated, and pseudomonas ovalis (ifo 3738) was found to have the highest activity of enzyme, which was inducibly formed by addition of l-methionine to the medium. l-methionine gamma-lyase, purified to homogeneity from ps. ovalis, has a molecular weight of about 173 000 and consists of nonidentical subunits (mol wt: 40 000 and 48 000). the enzyme exhibits absorption maxima a ...1977831771
magnitude of the equilibrium isotope effect on carbon-tritium bond synthesis.the exchange of tritium from 3hoh into the methyl group of pyruvate catalyzed by 6-phospho-2-keto-3-deoxygluconate aldolase (6-phospho-2-keto-3-deoxy-d-gluconate d-glyceraldehyde-3-phosphate-lyase, ec 4.1.2.14) of pseudomonas putida shows an equilibrium isotope effect of 0.78. from this value and the deuterium effect on the fumarase equilibrium (thomson, j.f. (1960) arch. biochem. biophys. 90, 1), one can calculate by use of the relative fractionation factors of hartshorn and shiner (hartshorn, ...1977836851
modified sutter's arginine dihydrolase medium for pseudomonas speciation.sutter's arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. bromothymol blue is the indicator for alkalinity in sutter's medium. by adding glucose and lowering the ph of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. the modified sutter's medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic ...1977838673
purification and properties of homoprotocatechuate 2,3-dioxygenase from bacillus stearothermophilus.the enzyme 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing) (homoprotocatechuate 2,3-dioxygenase) was purified from the thermophilic organism bacillus stearothermophilus, grown with j-hydroxyphenylacetic acid as a source of carbon. the enzyme appeared to be homogeneous as judged by disc-gel electrophoresis and sedimentation equilibrium measurements. the average molecular weight determined by three independent procedures was 106,000; the protein was globular and was dissociated ...1977838683
p-cymene pathway in pseudomonas putida: initial reactions.initial reactions of the p-cymene pathway induced in pseudomonas putida pl have been reinvestigated. oxidation of the methyl group attached to the nucleus occurs in three steps to give p-cumic acid. the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate) and not as previously postulated via 3-hydroxy-p-cumate. there are three pieces of evidence for the physiological rol ...1977845117
p-cymene pathway in pseudomonas putida: ring cleavage of 2,3-dihydroxy-p-cumate and subsequent reactions.it was confirmed that 2,3-dihydroxy-p-cumate is a substrate for ring cleavage in pseudomonas putida pl-w after growth with p-cymene or p-cumate. this compound was oxidized to pyruvate, acetaldehyde, isobutyrate, and carbon dioxide by extracts of cells, and these products appear in equimolar amounts. the transient appearance of compounds and 2,3-dihydroxy-p-cumate to a yellow intermediate (lambda max, 345 nm) without decarboxylation. extracts of the benzene nucleus; this is followed by decarboxyl ...1977845118
isolation of metabolic plasmid dna from pseudomonas putida. 1977849300
mandelate racemase from pseudomonas putida. absence of detectable intermolecular proton transfer accompanying racemization.an equimolar mixture of dl-[alpha-2h]- and dl-[alpha-13c]mandelate, when incubated with mandelate racemase (ec 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. similarly, an equimolar mixture of unlabeled and dl-[alpha-2h,alpha-13c]mandelate, when incubated with the enzyme, shows conversion of doubly labeled mandelate to singly labeled mandelate, ...1977849410
crystalline cis-benzene glycol dehydrogenase from pseudomonas putida. 1977859182
characterization of a spontaneously occurring mutant of the tol20 plasmid in pseudomonas putida mt20: possible regulatory implications.pseudomonas putida mt20 carries a plasmid (tol20) that codes for the enzymes responsible for the catabolism of toluene, m- and p-xylene to benzoate, and m- and p-toluate, respectively, followed by meta cleavage of the aromatic ring. growth on 5 mm benzoate selects very strongly for (i) strains that have been cured of the plasmid and (ii) strains with an intermediate growth pattern (the b3 phenotype) that retain the ability to grow on toluene, m-xylene, and benzoate but are unable to grow on m-to ...1977863853
distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...1977863854
molecular sizes and relationships of tol plasmids in pseudomonas.plasmid deoxyribonucleic acid was isolated from thirteen pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (tol plasmids). most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 x 10(6) to 202 x 10(6) daltons. some plasmids could be transformed into a pseudomonas putida strain to yield tol+ progeny. plasmids from 5 of the 13 st ...1977863855
myo-inositol transport system in pseudomonas putida.the kinetic features of the myo-inositol transport system in pseudomonas putida are reported. the system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. line-weaver-burk plots indicate the presence of two different systems, whose kt are 5 micrometer and 0.43 mm and whose v max are 7.9 and 27 nml/mg per min, respectively. transport activity of glucose-grown cells is very low. myo-inositol-grown cells lose the high-affinity sys ...1977893343
two modes of loss of the tol function from pseudomonas putida mt-2.some of a set of independently arising tol- (non toluate-utilising) derivatives of pseudomonas putida mt-2 have lost the unique plasmid present in the parent strain. in others this plasmid has suffered a deletion of a specific region of about 27 md.1977895716
molecular characterization of hydrocarbon degradative plasmids in pseudomonas putida. 1977901483
purification and partial amino acid sequence of the cyanogen bromide fragments of muconolactone isomerase from pseudomonas putida.muconolactone isomerase is shown to be resistant to proteolytic cleavage by trypsin. cyanogen bromide cleavage at the methionine residues of the polypeptide is at least 95% complete. six cyanogen bromide fragments are separated on deae-cellulose. one fragment is shown by amino acid analysis and carboxyl-terminal analysis to be an incomplete cleavage product. the five remaining fragments represent the entire polypeptide and have been ordered with respect to the entire muconolactone isomerase sequ ...1977901811
transposition of a beta-lactamase locus from rp1 into pseudomonas putida degradative plasmids.the beta-lactamase gene from the rp1 plasmid transposes into at least two pseudomonas putida degradative plasmids. donor strains that carry rp1 (bla+ tet+ apha+) and a degradative plasmid yield transconjugants that have only the bla+ marker of rp1. this occurs in up to 80% of all bla+ transconjugants. segregation of the bla+ marker requires the presence of a degradative plasmid in the donor and is only observed in transconjugants that have received degradative markers. the bla+ tet apha transcon ...1977584205
the purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from pseudomonas putida.the enzyme that catalyses the hydroxylation of the methyl group of p-cresol was purified from pseudomonas putida. it has mol.wt. 115000 and appears to contain two subunits of equal molecular weight. one subunit is a c-type cytochrome and the other is a flavoprotein. reduction of the cytochrome occurred on addition of substrate. the same enzyme catalyses both p-cresol hydroxylation and the further oxidation of the product, 4-hydroxybenzyl alcohol. the stoicheiometry of acceptor reduced per molecu ...1977588247
metabolism of dibenzothiophene by a beijerinckia species.beijerinckia b8/36 when grown with succinate in the presence of dibenzothiophene, accumulated (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene and dibenzothiophene-5-oxide in the culture medium. each metabolite was isolated in crystalline form and characterized by a variety of chemical techniques, cis-naphthalene dihydrodiol dehydrogenase, isolated from pseudomonas putida, oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene to a compound that was tentatively identified as 1,2-dihydroxydi ...1977596875
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
alpha-pinene metabolism by pseudomonas putida.by using metabolically altered mutants and acrylate, novel putative intermediates of alpha-pinene metabolism by pseudomonas putida pin11 were detected. they were characterized as 3-isopropylbut-3-enoic acid and (zeta)-2-methyl-5-isopropylhexa-2,5-dienoic acid.1977597274
a comparative study of the nah and tol catabolic plasmids in pseudomonas putida.a comparative study of the nah and tol catabolic plasmids was carried out to provide information for future genetic manipulation experiments involving these two plasmids. the plasmids were studied in a strain of p. putida and its mutant derivatives. the nah and tol plasmids were found to be incompatible. under the conditions used in these experiments the tol plasmid transferred into some strains into which nah was unable to transfer. the use of mutants to remove certain catabolic activities enco ...1977603460
in vitro inhibition of human peripheral blood lymphocyte transformation by an extract of pseudomonas putida.an extract prepared from a psychrophilic strain of pseudomonas putida was found to cause a dose-dependent inhibition of [h3]tdr incorporation into human peripheral blood lymphocytes stimulated with pha, cona, pwm, or in a mixed lymphocyte reaction. the inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. the extract was most effective when added prior to ...1977608686
microbiological transformations of terpenes: part xxiii--fermentation of geraniol, nerol & limonene by a soil pseudomonad, pseudomonas incognita (linalool strain). 1977612543
metabolism of d- and l-lactate by pseudomonas putida.pseudomonas putida grew at the same rate with the same molar growth yield on d-, l, or dl-lactate as the sole source of carbon for growth. d- and l- lactate were utilized simultaneously and at the same rate when the organism was grown on dl-lactate (ratio of d isomer to l isomer of 1:1). growth on either isomer alone, or in combination, caused the induction of both a d-lactate, and an l-lactate dehydrogenase. both enzymes were particulate and used dichlorophenolindophenol, or oxygen, but not na ...1977614007
isolation of mutants with altered metabolic control of the nah plasmid-encoded catechol meta-cleavage pathway.two types of mutants which displayed altered regulation of the nah catabolic plasmid-encoded catechol meta-cleavage pathway were isolated in pseudomonas putida. altered metabolic control was indicated by assay of catechol 2,3-dioxygenase. in one type of mutant the catechol 2,3-dioxygenase was synthesized constitutively. in the other type the range of carbon sources which induce the catechol 2,3-dioxygenase was increased.1977614009
microbiological transformations of terpenes: part xxiv--pathways of degradation of linalool, geraniol, nerol & limonene by pseudomonas incognita (linalool strain). 1977615106
products formed from analogues of 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (ddt) metabolites by pseudomonas putida.cultures of pseudomonas putida growing in solutions with diphenylmethane as sole carbon source formed 1,1,1',1'-tetraphenyldimethyl ether. the product was identified by gas chromatography, mass spectrometry, and infrared and nuclear magnetic resonance spectrometry. the formation of benzophenone, benzhydrol, and phenylglycolic acid was established by gas chromatography and mass spectrometry. similar techniques also revealed that phenylacetic acid was a major metabolite. resting cell suspensions c ...197716345181
bacteria--plant cell surface interactions: active immobilization of saprophytic bacteria in plant leaves.fibrillar structures, originating from the plant cell wall in the intercellular spaces of leaves of ;red kidney' bean, phaseolus vulgaris l., engulfed a saprophytic bacterium, pseudomonas putida, after its initial attachment to the host walls. phytopathogenic bacteria, pseudomonas phaseolicola and pseudomonas tomato, did not adhere to the plant cell wall nor were they encapsulated. bean lectins may be involved in the attachment and encapsulation processes.197717790770
changes in cytochrome content and electron transport patterns in pseudomonas putida as a function of growth phase.optical absorbance difference spectra of membrane vesicles prepared from aerobically grown pseudomonas putida indicated that, when harvested in logarithmic phase, the cells contained one c-type cytochrome and two or three b-type cytochromes, one of which was cytochrome o. as the cells grew into stationary phase and the oxygen concentration of the medium dropped to essentially zero, an additional component believed to be cytochrome d was produced. both the o- and d-type cytochromes might function ...1978618838
phosphonate utilization by bacteria.bacteria able to use at least one of 13 ionic alkylphosphonates of o-alkyl or o,o-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. four of these isolates used 2-aminoethylphosphonic acid (aep) as a sole carbon, nitrogen, and phosphorus source. none of the other phosphonates served as a carbon source for the organisms. one isolate, identified as pseudomonas putida, grew with aep as its sole carbon, nitrogen, and phosphorus source and released nearly all of the o ...1978618850
[characteristics of pseudomonas putida plasmid dnas].physico-chemical characteristics of plasmid dnas isoalted from pseudomonas putida g7 were studied as well as the behavior of these dnas in th eourse of chromatography on columns with sepharose 4b and kieselguhr with methylated albumin (mac). this strain was found to contain several plasmid dnas having molecular weights of 33-36x10(6), 15-18x10(6), and 3-5x10(6) dalton. the plasmid dnas of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is c ...1978651695
mutation to increased resistance to phenol in pseudomonas putida. 1978656570
regulation of the degradative pathway enzymes coded for by the tol plasmid (pwwo) from pseudomonas putida mt-2.pseudomonas putida mt-2 carries a plasmid (tol, pwwo) which codes for a single set of enzymes responsible for the catabolism of toluene and m- and p-xylene to central metabolites by way of benzoate and m- and p-toluate, respectively, and subsequently by a meta cleavage pathway. characterization of strains with mutations in structural genes of this pathway demonstrates that the inducers of the enzymes responsible for further degradation of m-toluate include m-xylene, m-methylbenzyl alcohol, and m ...1978659369
anthranilate synthetase component ii from pseudomonas putida. covalent structure and identification of the cysteine residue involved in catalysis.the complete amino acid sequence of carboxamidomethylated anthranilate synthetase component ii (as ii) from pseudomonas putida has been determined by analysis of cyanogen bromide fragments, tryptic peptides from the citraconylated protein, and by analysis of subdigests of these peptides. as ii is a single polypeptide chain of 197 residues having a calculated molecular weight of 21,684. previous studies (goto, y., keim, p. s., zalkin, h., and heinrikson, r. l. (1976) j. biol. chem, 251, 941-949) ...1978659439
[naphthalene oxidation by a pseudomonas putida strain carrying a mutant plasmid].naphthalene oxidation by a parent and a mutant strain of pseudomonas putida was studied. the parent strain contained a plasmid npl-1 which controlled oxidation of naphthalene to salicylic acid and was capable of oxidizing salicylate. the mutant strain did not oxidize salicylate because of a mutation in salicylate hydroxylase; it contained also a mutant plasmid npl-41 which determined constitutive synthesis of naphthalene oxygenase. salicylic acid which accumulated as a product of naphthalene cat ...1978661635
transformation of pseudomonas putida with chromosomal dna. 1978666840
magnetic circular dichroism of pseudomonas putida cytochrome p-450 in near infrared region.magnetic circular dichroism spectra of oxidized, reduced and carbonmonoxy reduced forms of cytochrome p-450 from d-camphor grown pseudomonas putida (p-450cam) were studied in the near infrared region (650 to 1200 nm) at various temperatures in the presence of d-camphor. oxidized p-450cam with camphor exhibited positive (+) and negative (-) magnetic cd bands at 825 and 970 nm, respectively, and both of them were assigned to faraday b terms. the magnetic cd spectrum of reduced p-450cam in the pres ...1978667105
nic, a conjugative nicotine-nicotinate degradative plasmid in pseudomonas convexa.the plasmid nature of genes specifying degradation of nicotine and nicotinate in pseudomas convexa strain 1 (pc1) is indicated by mitomycin curing and conjugational transfer to other strains. the nic plasmid appears to be compatible with other metabolic plasmids in pseudomonas putida.1978670150
the role of enoyl-coa hydratase in the metabolism of isoleucine by pseudomonas putida.the purpose of the present study was to determine if the enoyl coenzyme a hydratase formed by pseudomonas putida during growth on isoleucine was a unique enzyme specific for isoleucine metabolism. the highest levels of the hydratase were formed during growth on isoleucine intermediates and the lowest levels during growth on glutamate and glucose. data from growth experiments revealed that 2-methyl-3-hydroxybutyryl coenzyme a hydratase, an enzyme unique to isoleucine metabolism and enoyl coenzyme ...1978678016
pseudomonas ovalis ferredoxin: similarity to azotobacter and chromatium ferredoxins. 1978680138
chemical structure and biodegradability of halogenate aromatic compounds. substituent effects on 1,2-dioxygenation of benzoic acid.dioxygenation of substituted benzoic acids by whole cells of 3-chlorobenzoate-utilizing pseudomonas sp. b 13, benzoate-induced cells of alcaligenes eutrophus b 9 and toluate-grown cells of pseudomonas putida mt-2 was examined. electron-attracting substituents like halogen decreased the reaction rates of benzoate 1,2-dioxygenation. dioxygenation of substituted benzoic acids by p. putida mt-2 was mostly undisturbed by steric effects of the substituents. good correlation resulted between the log vr ...1978687664
amine dehydrogenase of pseudomonas putida: properties of the heme-prosthetic group.there was approximately five times more hemoprotein (amine dehydrogenase) in crude extracts obtained from pseudomonas putida grown on benzylamine than present in extracts from succinate-grown cells. the difference (reduced minus oxidized) spectrum of the purified enzyme possessed alpha,beta, and gamma bands at 550, 523, and 416 nm, respectively. the difference spectrum of the pyridine hemochrome derivative had absorption maxima at 416, 520, and 550 nm. these results, together with the fact that ...1978690082
a study of bacteria contaminating refrigerated cooked chicken; their spoilage potential and possible origin.cooked chicken was allowed to spoil in a normal kitchen refrigerator (variable temperature) and at a standard 4c. after 10 days' storage, bacteria were isolated from the chicken. it was found that the numbers of organisms at variable refrigeration temperature were tenfold higher than those at a uniform 4c. in an attempt to find the sources of contamination, swabs were made of different areas of the kitchen. many of the bacteria isolated from the spoiled chicken, were also isolated from the kitch ...1978701783
circular dichroism and magnetic circular dichroism of iron-sulfur proteins.circular dichroism (cd) and magnetic circular dichroism (mcd) spectra are reported for the 2-fe ferredoxins from pseudomonas putida and spirulina maxima, chromatium hipip, the 4-fe ferredoxin from bacillus stearothermophilus, and the 8-fe ferredoxin from clostridium pasteurianum. the spectral range spans the near-infrared, visible, and near ultraviolet. in all cases except oxidized 2-fe ferredoxins, electronic absorption is observed continuously from less than 5000 cm-1 to above 30,000 cm-1. the ...1978728385
chemical and spectral properties of putidamonooxin, the iron-containing and acid-labile-sulfur-containing monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida.gel chromatography indicates that putidamonooxin has a molecular weight of about 126,000. on the other hand, the amino acid composition and the iron-to-protein ratio point to a minimal molecular weight of 33,000 and 31,000 respectively. on sodium dodecylsulfate/polyacrylamide gel electrophoresis the enzyme migrated as a homogeneous band corresponding to a molecular weight of about 40,000. the number of spots found in the tryptic peptide map of the carboxymethylated and digested enzyme indicates ...1978729590
subunit and amino acid composition of l-arginine deiminase of pseudomonas putida. 1978729806
incorporation of [18o]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from pseudomonas putida.in the hydroxylation of the methyl group of p-cresol by an enzyme from pseudomonas putida the oxygen atom is derived from water. although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate.1978736904
p-cresol and 3,5-xylenol methylhydroxylases in pseudomonas putida n.c.i.b. 9896.pseudomonas putida n.c.i.b. 9869, when grown on 3,5-xylenol, hydroxylates the methyl groups on 3,5-xylenol and on p-cresol by two different enzymes. 3,5-xylenol methylhydroxylase, studied only in relatively crude extracts, requires nadh, is not active with p-cresol and is inhibited by cyanide, but not by co. the p-cresol methylhydroxylase requires an electron acceptor and will act under anaerobic conditions. it was purified and is a flavocytochrome c of mol.wt. approx. 114,000 consisting of two ...1978743215
the aromatic alcohol dehydrogenases in pseudomonas putida n.c.i.b. 9869 grown on 3,5-xylenol and p-cresol.whole cells of pseudomonas putida n.c.i.b 9869, when grown on either 3,5-xylenol or p-cresol, oxidized both m- and p-hydroxybenzyl alcohols. two distinct nad+-dependent m-hydroxybenzyl alcohol dehydrogenases were purified from cells grown on 3,5-xylenol. each is active with a range of aromatic alcohols, including both m- and p-hydroxybenzyl alcohol, but differ in their relative rates with the various substrates. an nad+-dependent alcohol dehydrogenase was also partially purified from p-cresol gr ...1978743216
evidence for a transmissible catabolic plasmid in pseudomonas putida encoding the degradation of p-cresol via the protocatechuate ortho cleavage pathway. 1978751853
tol plasmid in pseudomonas aeruginosa pao: thermosensitivity of self-maintenance and inhibition of host cell growth.the tol plasmid originally isolated in pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of pseudomonas, i.e., p. putida, p. fluorescens, and p. aeruginosa, except for a strain of p. aeruginosa, strain pao. the same strain, however, could accept the plasmid when its restriction and modification abilities were lost by mutations or by growing at high temperature. in addition, the transmissibility of the tol plasmid from strain pao to p. putida was low when the ...1978415040
rsf1010 plasmid as a potentially useful vector in pseudomonas species.rsf1010 plasmid dna was introduced into pseudomonas putida and p. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in pseudomonas species. the number of copies of rsf1010 was 43 per chromosome equivalent in p. putida cells.1978417070
isolation of tol and rp4 recombinants by integrative suppression.we obtained genetic and molecular evidence of non-thermosensitive recombinants of rp4 (kmr tcr cbr/apr) and the thermosensitive tol plasmid. as first isolated in pseudomonas aeruginosa pao, the recombinant plasmid ptn1 specified noninducible synthesis of tol enzymes and was transmissible to escherichia coli on selection for the transfer of kanamycin resistance. the phenotypic expression of tol genes of ptn1 in e. coli was low and also noninducible. a spontaneous segregant, ptn2, appearing from p ...1978418059
lipoate metabolism in pseudomonas putida lp: thiolsulfinates of lipoate and bisnorlipoate. 1978343716
bacterial catabolism of lipoic acid. isolation and identification of a methyl ketone.a soil bacterium, pseudomonas putida lp, can be grown on lipoate as sole source of carbon, sulfur, and energy. in addition to the previously identified catabolites (bisnorlipoate, tetranorlipoate, beta-hydroxybisnorlipoate, lipoate thiolsulfinate, and two bisnorlipoate thiolsulfinates) isolated from cultures of the organism grown on [1,6-14c[lipoate, a methyl ketone (1,2-dithiolane-3-butyl-3'-one) has now been isolated and identified. this catabolite was isolated by solvent extraction and hydrop ...1978357321
anabolic ornithine carbamoyltransferase of escherichia coli and catabolic ornithine carbamoyltransferase of pseudomonas putida. steady-state kinetic analysis.the anabolic and catabolic ornithine carbamoyltransferases of pseudomonas putida display an undirectional catalytic specialization: in citrulline synthesis for the anabolic enzyme, in citrulline phosphorolysis for the catabolic one. the irreversibility of the anabolic enzyme in vitro has been previously explained by its kinetic properties, whereas the irreversibility of the catabolic transferase in vivo was shown to be due to its allosteric behaviour. in this work a steady-state kinetic analysis ...1978359326
microbiological transformations of terpenes: part xxv--enzymes involved in the degradation of linalool in the pseudomonas incognita, linalool strain. 1978367951
amino acid sequence of a peptide containing an essential cysteine residue of escherichia coli gmp synthetase.the amino acid sequence of a 51-residue tryptic peptide of citraconylated [1-14c]carboxamidomethyl-labeled escherichia coli gmp synthetase was determined by sequenator analyses of the intact peptide and fragments obtained by cleavage of the peptide with cyanogen bromide, trypsin, and staphylcoccus aureus strain v8 protease. the cysteine residue of this peptide fragment is essential for glutamine-dependent gmp synthesis activity and is implicated in formation of a hypothetical covalent glutamyl-e ...1978213434
characterization of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1.nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, was purified to homogeneity, as judged by sodium dodecyl sulfate disc gel electrophoresis and ultracentrifugation, from benzoate-induced cells of pseudomonas arvilla. the molecular weight of the enzyme was determined to be 38,300 by sedimentation equilibrium analysis, 37,000 by sephadex g-100 gel filtration, and 37,500 by sodium dodecyl sulfate disc gel electrophoresis, respectively, indicating that the enzyme consisted ...1978214433
[regulation and properties of a particular acceptor-dependent alcohol dehydrogenase of pseudomonas putida during growth on n-alkanes]. 1978216166
purification and properties of 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase from two strains of pseudomonas putida.growth on phenol of two strains of pseudomonas putida biotype a, ncib 10015 and ncib 9865, elicits the synthesis of an enzyme that hydrolyzes 2-hydroxy-6-oxo-2,4-heptadienoate to 2-oxopent-4-enoate. the purified enzyme from pseudomonas ncib 10015 has a molecular weight of 118,000 and dissociates in sodium dodecyl sulfate to a species of molecular weight 27,700; the enzyme from pseudomonas ncib 9865 has a molecular weight of 100,000 and dissociates to a species of 25,000 molecular weight. the hyd ...197877272
isolation of large bacterial plasmids and characterization of the p2 incompatibility group plasmids pmg1 and pmg5.large plasmids from agrobacterium tumefaciens, salmonella typhimurium, escherichia coli, pseudomonas putida, and pseudomonas aeruginosa were routinely and consistently isolated using a procedure which does not require ultracentrifugation but includes steps designed to separate large-plasmid dna from the bacterial folded chromosome. it also selectively removes fragments of broken chromosome. a variety of large plasmids was readily visualized with agarose gel electorphoresis, including five betwee ...197897269
tol is a broad-host-range plasmid.we readily isolated insertions of the carbenicillin resistance element tn401 into the tol plasmid in pseudomonas putida. hybrid tol::tn401 plasmids stably express the cbr phenotype in pseudomonas aeruginosa and escherichia coli. whereas the replicative and conjugative functions are expressed in both hosts, the ability to grow on m-toluate is only expressed in the pseudomonas species.197897271
a study on the reconstitution of iron-superoxide dismutase from pseudomonas ovalis. 197825273
the purification and properties of urocanase from pseudomonas testosteroni.urocanase (urocanate hydratase, ec 4.2.1.49) purified from pseudomonas testosteroni has a mol.wt. of 118000 determined by sedimentation-equilibrium analysis. ultracentrifugation in 6m-guanidine hydrochloride and polyacrylamide-gel electrophoresis in sodium dodecyl sulphate show that the enzyme consists of two identical or very similar subunits. it is, like urocanase isolated from other sources, inhibited by reagents that react with carbonyl groups. although urocanase from ps. testosteroni is str ...197825660
purification and characterization of a heme-containing amine dehydrogenase from pseudomonas putida.the primary amine dehydrogenase of pseudomonas putida np was purified to homogeneity as judged by polyacrylamide gel electrophoresis. cytochrome c or an artificial electron acceptor was required for amine dehydrogenase activity. the enzyme was nonspecific, readily oxidizing primary monoamines, benzylamine, and tyramine; little or no measurable activity was detected with isoamines, l-ornithine, l-lysine, and certain diamines or polyamines. the ph optima for n-butylamine, benzylamine, and n-propyl ...197826667
photoactivation of urocanase in pseudomonas putida. role of sulfite in enzyme modification. 197829899
[interrelationships between carnitine metabolism and fatty acid assimilation in pseudomonas putida (author's transl)].the carnitine metabolism and some relations to the fatty acid metabolism were studied in pseudomonas putida by means of control of growth, analysis of metabolites, and determination of enzyme activites. the strain grew on gamma-butyrobetaine, d,l- and l-carnitine, glycinebetaine, choline, d,l-norcarnitine, d,l-gamma-amino-beta-hydroxybutyrate, and d,l-beta-hydroxybuty-rate. although the strain used straight-chain fatty acids of 2-16 c-atoms, it was only able to grow on o-acyl-l-carnitines of 10 ...1978565193
formaldehyde dehydrogenase from pseudomonas putida. purification and some properties.formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of pseudomonas putida c-83 by chromatographies on columns of deae-cellulose, deae-sephadex a-50, and hydroxyapatite. the purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at ph 7.8 using formaldehyde as a substrate. the enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. the enzyme w ...1979571868
stereochemistry of the conversion of methacrylate to beta-hydroxyisobutyrate in pseudomonas putida. 1979572832
stereospecific hydrogen loss in the conversion of [2h7]isobutyrate to beta-hydroxyisobutyrate in pseudomonas putida. the stereochemistry of beta-hydroxyisobutyrate dehydrogenase. 1979573276
deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.plasmid and phage deoxyribonucleic acid (dna) harboring bacterial insertion sequence (is) elements is1, is2, and is5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. the hybridization method used permits the detection of sequences partially homologous to the elements. hybridization of the is-containing probes to each other revealed a region of limited homology between is1 and is2. homologous sequences were then detected by comput ...1979159291
identification of the prosthetic group of urocanase. the mode of its reaction with sodium borohydride and of its photochemical reactivation.urocanase from pseudomonas putida and from beef liver were isolated by modifying described procedures. both enzymes were inactivated and labeled on treatment with tritiated sodium borohydride and gave, upon subsequent hydrolysis, a radioactive acid. the previously reported identity of this acid as 2-hydroxybutanoic acid was disproved by several criteria. other hydroxy acids were also proved to be different from the radioactive acid derived from urocanase. a large portion of the radioactive mater ...197933176
[expression of deo-operon escherichia coli k-12 genes in the makeup of hybrid plasmid rp4::mu-deo in pseudomonas trifolii and pseudomonas putida]. 1979113191
comparison of microsomal and solubilized monooxygenases from rat and rabbit by proton magnetic relaxation.the paper presents results of a comparative study of the haem environment, by proton magnetic relaxation, in p-450 and p-448 monooxygenases from rat and rabbit, induced by phenobarbital and 3-methylcholanthrene, in both species. it was established that the method yields information on the accessibility of the haem iron for solvent molecules (protons), both in microsomes and in solubilized samples of various degrees of purification, i.e. association. the state of micelles in the solutions does no ...1979229678
a cleavage map of the tol plasmid of pseudomonas putida mt-2.a cleavage map of the tol plasmid pwwo has been determined for the restriction endonucleases hindiii and xhoi. a number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified xhoi fragments to southern blots of hindiii digest products and (iii) analysis of a number of deletion mutants.1979231727
chromosomal mobilization from a reca mutant of pseudomonas putida.a methyl methane sulfonate sensitive mutant of p. putida strain ppg1 is also extremely sensitive to uv-rays, compared to parent wild type cells. this mutant behaves typically as recombination less (reca) mutants of escherichia coli and pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. sex factor plasmids such as k-xyl or tol can mobilize chromosomal genes equally well bot ...1979232230
expression of escherichia coli tryptophan operon in rhizobium leguminosarum.rp4-trp hybrid plasmid containing escherichia coli whole tryptophan operon was conjugatively transferred from e. coli to rhizobium leguminosarum strains carrying mutations in different trp genes, converting their trp- phenotype to trp+. that the phenotype change of the r. leguminosarum cells was due to the presence of the e. coli tryptophan operon was verified by the isolation of rp4-trp hybrid plasmid from the r. leguminosarum conjugant cells, and by re-transfer of rp4-trp plasmid by conjugatio ...1979375025
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