Publications

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d- and l-isoleucine metabolism and regulation of their pathways in pseudomonas putida.pseudomonas putida oxidized isoleucine to acetyl-coenzyme a (coa) and propionyl-coa by a pathway which involved deamination of d-isoleucine by oxidation and l-isoleucine by transamination, oxidative decarboxylation, and beta oxidation at the ethyl side chain. at least three separate inductive events were required to form all of the enzymes of the pathway: d-amino acid dehydrogenase was induced during growth in the presence of d-isoleucine; branched-chain keto dehydrogenase was induced during gro ...19744150713
regulation of leucine catabolism in pseudomonas putida.the generation time of pseudomonas putida with l-leucine was 20 h in synthetic media but only 3 h with d-leucine. slow growth in the presence of l-leucine was partially overcome by addition of 0.1 mm amounts of either d-valine, l-valine, or 2-ketoisovalerate. the activities of five enzymes which take part in the oxidation of leucine by p. putida were measured under various conditions of growth. four enzymes were induced by growth with dl-leucine as sole source of carbon: d-amino acid dehydrogena ...19744150714
effect of temperature on histidine ammonia-lyase from a psychrophile, pseudomonas putida.pseudomonas putida was able to grow at 0 c in a complex medium containing l-histidine and to synthesize histidine ammonia-lyase and urocanase. the activity of the former enzyme was assessed between -10 and 60 c in cells and in cell extracts. activity was maximal from 20 to 35 c. below 20 c, activity decreased with temperature but, significantly, the enzyme exhibited 30% of its maximal activity at 1.5 c. the temperature response was similar in both intact cells and cell extracts, which indicated ...19744152044
involvement of threonine dehydratase in biosynthesis of the alpha-ketobutyrate prosthetic group of urocanase.seventeen mutants of pseudomonas putida that were unable to grow on threonine as nitrogen source owing to a lack of threonine dehydratase were isolated, and all were found to be unable to synthesize active urocanase. spontaneous revertants selected for urocanase production concomitantly regained threonine dehydratase. mutants that were unable to utilize urocanate as carbon source were also isolated, and these were defective in urocanase formation but were normal in threonine dehydratase levels. ...19744154935
d-lysine catabolic pathway in pseudomonas putida: interrelations with l-lysine catabolism.the isolation of several mutant strains blocked in l-lysine degradation has permitted an assessment of the physiological significance of enzymatic reactions related to lysine metabolism in pseudomonas putida. additional studies with intact cells involved labeling of metabolic intermediates from radioactive l- or d-lysine, and patterns of enzyme induction in both wild-type and mutant strains. these studies lead to the conclusions that from l-lysine, the obligatory pathway is via delta-aminovalera ...19744359655
pseudomonas putida cytochrome p-450. binding of a spin-labeled analog of the inhibitor metyrapone. 19744364068
purification and propeties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of pseudomonas putida.cells of pseudomonas putida, after growth with naphthalene as sole source of carbon and energy, contain an enzyme that oxidizes (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. the purified enzyme has a molecular weight of 102,000 and apparently consists of four 25,500 molecular weight subunits. the enzyme is specific for nicotinamide adenine dinucleotide as an electron acceptor and also oxidizes several other cis-dihydrodiols. however, no enzymatic activity was ob ...19744369091
a mutant of pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. 19744371622
d-alpha-hydroxyglutarate dehydrogenase of rhodospirillum rubrum.d-alpha-hydroxyglutarate dehydrogenase of r. rubrum grown anaerobically in the light was partially purified and some properties were investigated. 1. the enzyme catalyze stoichiometrically the dehydrogenation reaction of d-alpha-hydroxyglutarate into alpha-oxoglutarate, coupled with the reduction of 2, 6-dichlorophenolindophenol. 2. cytochrome c2, cytochrome c, and ferricyanide are effective as electron acceptors with the crude enzyme but not with the purified one, whereas nad+ and nadp+ are com ...19755424
immunological study of anthranilate synthetase.an immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ...197550316
mandelate racemase from pseudomonas putida. magnetic resonance and kinetic studies of the mechanism of catalysis.the interactions of mandelate racemase with divalent metal ion, substrate, and competitive inhibitors were investigated. the enzyme was found by electron paramagnetic resonance (epr) to bind 0.9 mn2+ ion per subunit with a dissociation constant of 8 mum, in agreement with its kinetically determined activator constant. also, six additional mn2+ ions were found to bind to the enzyme, much more weakly, with a dissociation constant of 1.5 mm. binding to the enzyme at the tight site enhances the effe ...1975164210
magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b-5 and cytochrome p-450-cam.magnetic circular dichroism spectra are reported for the visible and near ultraviolet spectral regions of liver microsomes from dimethylbenzanthracene-treated rats. the sequential addition of nadh, dithionite, and carbon monoxide enables us to determine contributions to the magnetic circular dichroism by cytochromes b-5 and p-450, which dominate the spectra. the magnetic circular dichroism of the microsomal preparation is compared with that of purified oxidized and reduced cytochrome -b-5 from p ...1975164936
physiological role for the membrane bound ascorbate-tmpd oxidase in pseudomonas putida.the activity of the membrane-bound ascorbate-tmpd oxidase in pseudomonas putida varies with growth conditions and age of the culture. a comparison of the effects of cyanide and azide on the oxidation of various substrates suggests that ascorbate-tmpd oxidase is not the terminal oxidase for nadh or succinate oxidation. however, it does have a role in the oxidation of nicotinate, and may act as an additional terminal oxidase under certain other growth conditions.1975168828
initial reactions in the oxidation of naphthalene by pseudomonas putida.a strain of pseudomonas putida that can utilize naphthalene as its sole source of carbon and energy was isolated from soil. a mutant strain of this organism, p. putida 119, when grown on glucose in the presence of naphthalene, accumulates optically pure (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene in the culture medium. the cis relative stereochemistry in this molecule was established by nuclear magnetic resonance spectrometry. radiochemical trapping experiments established that this cis d ...1975234247
catalytic and thermodynamic properties of the urocanate hydratase reaction.urocanate hydratase (4-imidazolone-5-propionate hydro-lyase, ec 4.2.1.49) isolated from pseudomonas putida contains covalently bound alpha-ketobutyrate as its cofactor. in the process of examining the mechanism by which alpha-ketobutyrate serves in this capacity, various thermodynamic parameters and temperature effects on urocanate hydratase activity were determined. as the equilibrium constant at 15 degrees c for imidazooone propionate formation from urocanate is approximately 69, regardless of ...1975235308
metabolism of resorcinylic compounds by bacteria. purification and properties of acetylpyruvate hydrolase from pseudomonas putida 01.acetylpyruvate hydrolase, the terminal inducible enzyme of the pathway of orcinol catabolism in pseudomonas putida, catalyzes the quantitative conversion of acetylpyruvate into acetate and pyruvate. the enzyme has been purified approximately 40-fold from extracts of ps. putida grown on orcinol. disc gel electrophoresis of the preparations show one major and one minor band of protein. the molecular weight of the enzyme is approximately 38,000 by sodium dodecyl sulfate electrophoresis. acetylpyruv ...1975236305
aromatic aldehyde dehydrogenase from pseudomonas putida n.c.i.b. 9869. 1975236959
2-keto-3-deoxy-6-phosphogluconic aldolase from pseudomonas putida. 1975237184
allantoin racemase: a new enzyme from pseudomonas species.1. allantoin racemase is a novel enzyme which catalyzes the conversion of s(+)-and r(minus)-allantoin into the racemate. 2. the enzyme is present in pseudomonas testosteroni, pseudomonas putida and five biotypes of pseudomonas fluorescens, but absent in a number of other pseudomonas species. 3. the enzyme of ps. testosteroni was purified 133-fold and exposes optimal activity at ph 8.0-8.2 and 50 degrees c. the enzyme is stable on heating for 15 min at 70 degrees c. 4. the enzyme appeared to be s ...1975237557
crystallization and properties of l-arginine deiminase of pseudomonas putida.crystalline l-arginine deiminase of pseudomonas putida was prepared by the following steps: sonic disruption, ammonium sulfate fractionation, protamine sulfate treatment, deae-cellulose column chromatography, and l-arginine-sepharose 6b chromatography followed by crystallization. this procedure yields a crystalline pure enzyme with a 45% recovery of the activity in crude cell-free extracts. the yield is significantly higher than that reported for this enzyme. the purified enzyme appears to be ho ...1975237904
intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the l-arginine catabolic pathway of pseudomonas putida.in pseudomonas putida p2 grown on l-arginine as the sole source of carbon and nitrogen, catabolism of l-arginine forms of alpha-ketoarginine, gamma-guanidinobutyrate, and gamma-aminobutyrate. a previously undetected intermediate, gamma-guanidinobutyraldehyde, is identified as the product of alpha-ketoarginine decarboxylase. an 86-fold purification of this enzyme is described. activity is thiamine pyrophosphate-dependent and cofactor reassociation is facilitated by divalent cations. the order of ...1975237915
extradiol cleavage of 3-substituted catechols by an intradiol dioxygenase, pyrocatechase, from a pseudomonad.pyrocatechase (catechol 1,2-oxidoreductase (decyclizing), ec 1.13.11.1), a ferric ion-containing dioxygenase from pseudomonas arvilla c-1, catalyzes the intradiol cleavage of catechol with insertion of 2 atoms of molecular oxygen to form cis,cis-muconic acid. the enzyme also catalyzed the oxidation of various catechol derivatives, including 4-methylcatechol, 4-chlorocatechol, 4-formylcatechol (protocatechualdehyde), 4,5-dichlorocatechol, 3,5-dichlorocatechol, 3-methylcatechol, 3-methoxycatechol, ...1975238971
a 4-methoxybenzoate o-demethylase from pseudomonas putida. a new type of monooxygenase system.a strain of pseudomonas putida grown on 4-methoxybenzoate as sole carbon source contains an enzyme system for the o-demethylation of this substrate. the enzyme system is purifiable and can be separated into two components: an nadh-dependent reductase and an iron-containing and acid-labile-sulfur-containing monooxygenase. the reductase, of molecular weight 42000 and containing two chromophores, an fmn and an iron-sulfur complex (epr at g = 1.95), reduces both one-electron and two-electron accepto ...1975240720
physiological function of the pseudomonas putida ppg6 (pseudomonas oleovorans) alkane hydroxylase: monoterminal oxidation of alkanes and fatty acids.pseudomonas putida ppg6 is able to utilize purified n-alkanes of six to ten carbon atoms for growth. it can also grow on the primary terminal oxidation products of these alkanes and on 1-dodecanol but not on the corresponding 2-ketones or 1,6-hexanediol, adipic acid, or pimelic acid. revertible point mutants can be isolated which have simultaneously lost the ability to grow on all five n-alkane growth substrates but which can still grow on octanol or nonanol. an acetate-negative mutant defective ...1975804473
autogenous regulation of the inducible tryptophan synthase of pseudomonas putida.mutants blocked before indole-3-glycerol phosphate formation in the tryptophan biosynthetic pathway of p. putida ("early-blocked" mutants) are unable to use indole as a source of tryptophan for growth on minimal medium. the uninduced level of tryptophan synthase [ec 4.2.1.20; l-serine hydro-lyase (adding indole)] in such mutants was thought to be responsible for this property. we have shown that levels of indole higher than those previously tested will support growth of these mutants. in additio ...19751055401
characterization of the growth of pseudomonas putida lp on lipoate and its analogues: transport, oxidation, sulphur source, and enzyme induction.pseudomonas putida lp, which grows on lipoate, nh4no3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-coa. d-, l-, or dl-lipoate serve equally well as carbon and sulphur sources. there was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortened analogues, but these, as well as lipoate, could supply the sulphur needed for growth when acetate was ...19751089758
lipoate metabolism in pseudomonas putida lp. 19751099990
transformation of pseudomonas putida and escherichia coli with plasmid-linked drug-resistance factor dna.conditions optimal for the transformation of pseudomonas putida and e. coli with a drug-resistance factor (rp 1) dna, which specifies resistance to carbenicillin, tetracycline, kanamycin, and neomycin, are described. the transformants retain all the fertility, incompatibility, and drug-resistance characteristics present in the parent. covalently-closed circular molecules of almost identical contour lengths have been isolated from the parent and the transformants. the frequency of transformation ...19751103151
improved method of selection for mutants of pseudomonas putida.optimum conditions for enrichment of mutants of pseudomonas putida in liquid culture were established using a procedure which combines n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis with an improved d-cycloserine selection.19751108792
purification and characterization of bacteriophage gh-i-induced deoxyribonucleic acid-dependent ribonucleic acid polymerase from pseudomonas putida.infection of pseudomonas putida by the bacteriophage gh-l-induced the synthesis of a novel dna-dependent rna polymerase. this gh-l-induced rna polymerase was purified to near homogeneity. it was shown to be distinct from the host rna polymerase (alpha-2 beta beta sigma) physically and in respect to many of its catalytic properties. the gh-l-induced rna polymerase was composed of a single polypeptide of approximately 98,000 molecular weight. the divalent metal ion requirement for in vitro rna syn ...19751112826
the uptake of fructose by pseudomonas putida.fructose transport was not apparently affected in a number of pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturation kinetics (apparent km equal 0.3 mm). the fructose uptake system serves to transport glucose in addition to fructose. the entry of fructose into p.putida cells appears to be media ...19751115560
pathways for the degradation of m-cresol and p-cresol by pseudomonas putida.a comparison of the oxidation rates of various compounds by whole cells of pseudomonas putida 3, 5 indicated that m-cresol is metabolized by oxidation to 3-hydroxybenzoate followed by hydroxylation to gentisate, the ring-fission substrate, when grown with 3, 5-xylenol. however, when m-cresol was the growth substrate, similar experiments suggested a different pathway involving a methyl-substituted catechol, and ring-fission by meta cleavage. assays of ring-fission enzymes in cell-free extracts co ...19751123316
metabolism of resorcinylic compounds by bacteria. purification and properties of orcinol hydroxylase from pseudomonas putida 01.orcinol hydroxylase (ec 1.14.13.6), which catalyzes the first reaction of orcinol catabolism in pseudomonas putida 01, has been purified to homogeneity, and crystallized. orcinol hydroxylase catalyzes the hydroxylation of orcinol with equimolar consumption of o2 and nadh (or nadph) to 2, 3, 5-trihydroxytoluene, which is nonenzymically oxidized to a quinone. the visible absorption spectrum of the enzyme shows maxima at 373 and 454 nm and a shoulder at 480 nm. fad can be dissociated from the prote ...19751126936
the uptake of glucose and gluconate by pseudomonas putida.the uptake of glucose and gluconate is under inductive control in pseudomonas putida. glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. glucose and gluconate uptake obeyed saturation kinetics: the apparent km for glucose was 6 mm and that for gluconate was 0.5 mm. therefore, transport of both substrates appears to be mediated by enzyme-like carriers. glucose and gluconate are parallel inhibitors for their uptake9 strains selected for their ...19751134500
regulation of alkane oxidation in pseudomonas putida.we have studied the appearance of whole-cell oxidizing activity for n-alkanes and their oxidation products in strains of pseudomonas putida carrying the oct plasmid. our results indicate that the oct plasmid codes for inducible alkane-hydroxylating and primary alcohol-dehydrogenating activities and that the chromosome codes for constitutive oxidizing activities for primary alcohols, aliphatic aldehydes, and fatty acids. mutant isolation confirms the presence of an alcohol dehydrogenase locus on ...19751150626
induction of alkane hydroxylase proteins by unoxidized alkane in pseudomonas putida.in vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity.19751150630
immunochemical and compositional comparison of cytochrome p-450 cam of pseudomonas putida and p-450 lm of phenobarbital-induced rabbit liver microsomes.although highly purified cytochrome p-450 of pseudomonas putida (p-450 cam), and that from phenobarbital-induced rabbit liver microsomes (p-450 lm), differ markedly in their catalytic and physical properties, they show immunological cross reaction by competitive binding and inhibition of catalytic activity, and are of similar amino acid composition. upon treatment with cyanogen bromide they yield small heme-containing peptides of highly similar amino acid composition.19751155253
metabolism of n-methylpurines by a pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen.pseudomonas putida, strain 40, originally isolated by enrichment on caffeine as the sole source of carbon and nitrogen, has been developed to grow on 0.5% caffeine. the organism will grow on any n-methyl derivative of xanthine containing one or more methyl groups at the 1, 3, or 7 positions. an investigation of the activities of resting cell suspensions and cell-free preparations together with the detection of metabolic intermediates suggest that caffeine is first metabolized by the action of an ...19751158847
pseudomonas putida cytochrome p-450. the effect of complexes of the ferric hemeprotein on the relaxation of solvent water protons.with pulsed nuclear magnetic resonance techniques, the effects of various complexes of ferric cytochrome p-450 on the relaxation rate of bulk solution water protons have been determined. for the camphor, metyrapone, and 4-phenylimidazole complexes, the experimental results are consistent with outer sphere relaxation effects. however, for the substrate-free enzyme, the magnitude and temperature dependence of the paramagnetic relaxation effects indicate the presence of exchangeable protons in the ...19751158868
the stereochemistry at carbon 3 of pyruvate lyase condensation products. 2-keto-3-deoxygluconate-6-phosphate aldolase.in a condensation between [3-3h3]pyruvate and d-glyceraldehyde-3-p as catalyzed by 2-keto-3-deoxygluconate-6-p aldolase (ec 4.1.2.14) of pseudomonas putida, c--c synthesis occurred appreciably faster than c--3h bond breaking. since tritium is present in tritiated pyruvate in tracer amounts, this result showed hydrogen isotope discrimination in pyruvate deprotonation and suggests enolpyruvate generation to be at least partially rate-limiting in the condensation reaction. consequently, in a conden ...19751158885
metabolism of toluene and xylenes by pseudomonas (putida (arvilla) mt-2: evidence for a new function of the tol plasmid.pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m-xylene, and p-xylene on a transmissible plasmid, tol. these compounds are degraded by oxidation of one of the methyl substituents via the corresponding alcohols and aldehydes to benzoate and m- and p-toluates, respectively, which are then further metabolised by the meta pathway, also coded for by the tol plasmid. the specificities of the benzyl alcohol dehydrogenase and the benzaldehyde dehydrogenase for their three ...19751176436
[arthrobacter siderocapsulatus isolated from lake water].two microbial strains have been isolated from lake water. the strains oxidize ferrous compounds and manganese. by the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus siderocapsa. however, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus arthrobacter (corynebacteriaceae). since these microorganisms differ, by their cultural and morpho-physiological properties, f ...19751177780
mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450.anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ...19751182094
mössbauer investigations of high-spin ferrous heme proteins. ii. chloroperoxidase, horseradish peroxidase, and hemoglobin.reduced samples of chloroperoxidase, horseradish peroxidase, and deoxyhemoglobin were studied by mössbauer spectroscopy in strong magnetic fields. the intricate paramagnetic spectra of chloroperoxidase were evaluated in detail in the framework of a spin hamiltonian pertinent to high-spin ferrous iron. the studies strongly suggest that, in their reduced states, chloroperoxidase from caldariomyces fumago and cytochrome p-450 from pseudomonas putida have similar, if not identical ligand structures ...19751182095
new mechanisms for the biosynthesis and metabolism of 2-keto-l-gulonic acid in bacteria.l-sorbose is oxidized to 2-keto-l-gulonic acid (kga) via the following sequence of reactions which we call the "sorbosone pathway": l-sorbose in equilibrium l-sorbosone leads to kga. the first step is reversible and is mediated by enzymes found in a soluble fraction obtained from pseudomonas putida atcc 21812. although no cofactor requirements were found for the forward reaction, the reverse reaction clearly required nadh. enzymes for this nadh-dependent synthesis of l-sorbose could be different ...19751182275
an inducible amidase from pseudomonas striata. 19751191288
a kinetic and structural characterization of adenosine-5'-triphosphate: ribonucleic acid adenylyltransferase from pseudomonas putida.a catalytic and structural study of atp:rna adenylyltransferase (ec 2.7.7.19) from the particulate fraction of pseudomonas putida was made. during the large-scale purification of this enzyme, designated adenylyltransferase b, a previously undetected atp-incorporating activity, designated adenylyltransferase a, was observed. adenylyltransferases a and b were indistinguishable catalytically; however, they differed in their chromatographic and sedimentation properties. adenylyltransferases a and b ...19751191706
physiological consequences of starvation in pseudomonas putida: degradation of intracellular protein and loss of activity of the inducible enzymes of l-arginine catabolism.we investigated the degradation of radioisotopically labeled intracellular protein in starved, intact cells of pseudomonas putida p2 (atcc 25571) and the regulation of this process. intracellular protein isotopically labeled with l-[4,5-3h]leucine during log-phase growth at 30 c is degraded at rates of 1 to 2%/h in log-phase cells and 7 to 9%/h in starved cells. rifampin, chloramphenicol, and tosyllysine chloromethylketone lower the rate of protein degradation by starved cells. addition to starv ...19751194237
alternative routes of aromatic catabolism in pseudomonas acidovorans and pseudomonas putida: gallic acid as a substrate and inhibitor of dioxygenases.when 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) was added to pseudomonase acidovorans growing at the expense of succinate, enzymes required for degrading homoprotocatechuate to pyruvate and succinate semialdehyde were strongly induced. these enzymes were effectively absent from cell extracts of the organism grown with 4-hydroxyphenylacetic acid, and this substrate was metabolized by the catabolic enzymes of the homogentisate pathway. two separate ring-fission dioxygenases for 3,4,5 ...19751194238
the stereospecificity of sequential nicotinamide-adenine dinucleotide-dependent oxidoreductases in relation to the evolution of metabolic sequences.the generalization that 'when a metabolic sequence involves consecutive nicotinamide-adenine dinucleotide-dependent reactions, the dehydrogenases have the same stereospecificity' was tested and confirmed for three metabolic sequences. (1) nad+-xylitol (d-xylulose) dehydrogenase and nadp+-xylitol (l-xylulose) dehydrogenase are both b-specific. (2) d-mannitol 1-phosphate dehydrogenase and d-sorbitol 6-phosphate dehydrogenase are both b-specific. (3) meso tartrate dehydrogenase and oxaloglycollate ...19751200995
host factor for coliphage qbeta rna replication is present in pseudomonas putida.host factor (hf)1, is a 12000 molecular weight polypeptide that is found in uninfected escherichia coli and is required as a hexamer along with qbeta replicase for in vitro replication of qbeta phage rna. it has recently been found to be associated with ribosomes and to bind tightly to poly(a). we report here the identification and purification of hf from pseudomonas putida. hf can be detected in crude extracts by both functional activity in the qbeta rna replication assay and by immunodiffusion ...19751207667
exchange reactions catalyzed by methioninase from pseudomonas putida.highly purified methioninase from pseudomonas putida, which catalyzes alpha, gamma-elimination reactions of homocysteine and its s-substituted derivatives as well as alpha, beta-elimination reactions of cysteine and its derivatives, was found to catalyze exchange reactions between the substituent at the gamma-carbon of homocysteine substrates and exogenously added alkanethiols, forming the corresponding s-alkylhomocysteines. it also catalyzed similar beta-exchange reactions between cysteine and ...19751213994
dynamic and steady state studies of phenol biodegradation in pure and mixed cultures.the microbial degradation of phenol by pure and mixed cultures of pseudomonas putida was studied in batch, phenol-stat, and continuous culture systems. in the continuous culture runs, both steady state and transient experiments were performed. from these experiments, a model for the kinetic behavior of the organisms was evolved and an analysis performed on the stability and dynamic behavior of pure and mixed cultures. the results indicate that it should be possible to achieve phenol removal from ...19751236402
[r factor found in pseudomonas putida]. 19751240230
role and regulation of the ortho and meta pathways of catechol metabolism in pseudomonads metabolizing naphthalene and salicylate.the enzymes of naphthalene metabolism are induced in pseudomonas putida atcc 17484, ppg7, ncib 9816, and pg and in pseudomonas sp. atcc 17483 during growth on naphthalene or salicylate; 2-aminobenzoate is a gratuitous inducer of these enzymes. the meta-pathway enzymes of catechol metabolism are induced in atcc 17483 and ppg7 during growth on naphthalene or salicylate or during growth in the presence of 2-aminobenzoate, but in atcc 17484 and ncib 9816 the ortho-pathway enzymes of catechol metabol ...19761245462
properties of an inducible uptake system for beta-ketoadipate in pseudomonas putida.wild-type strains of pseudomonas putida form an inducible uptake system that appears to act on beta-ketoadipate under normal physiological conditions. the system is induced by beta-ketoadipate and is represented by catabolites derived from it. adipate is metabolized very slowly by wild-type p. putida cultures; [14c]adipate was used as an analogue of beta-ketoadipate to measure the transport activity in wild-type cells and in cells that constitutively produced the uptake system. constitutive cell ...19761245464
purification, crystallization and properties of iron-containing superoxide dismutase from pseudomonas ovalis.three electrophoretically distinct superoxide dismutases (ec 1.15.1.1) were observed in the crude extracts from pseudomonas ovalis. one of these was isolated as an iron-containing superoxide dismutase. it contained 1.4 gatoms of fe per mol of enzyme, and had a specific activity of 3900 units per mg of protein. a crystallized enzyme contained 1.1 gatoms of fe per mol of enzyme, and had a specific activity of 3100 units per mg of protein. the results of sedimentation equilibrium and gel filtration ...19761247598
ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence of new tol plasmids.thirteen bacteria have been isolated from nine different soil samples by selective enrichment culture on m-toluate (m-methylbenzoate) minimal medium. eight of these were classified as pseudomonas putida, one as a fluorescent pseudomonas sp., and four as nonfluorescent pseudomonas sp. all 13 strains appeared to carry tol plasmids superficially similar to that previously described in p. putida mt-2 in that: (i) all the wild-type strains could utilize toluene, m-xylene, and p-xylene as sole carbon ...19761254555
metabolism of resorcinylic compounds by bacteria: orcinol pathway in pseudomonas putida.enrichment cultures yielded two strains of pseudomonas putida capable of growth with orcinol (3,5-dihydroxytoluene) as the sole source of carbon. experiments with cell suspensions and cell extracts indicate that orcinol is metabolized by hydroxylation of the benzene ring followed successively by ring cleavage and hydrolyses to give 2 mol of acetate and 1 mol of pyruvate per mol of orcinol as shown: orcinol leads to 2,3,5-trihydroxytoluene leads to 2,4,6-trioxoheptanoate leads to acetate + acetyl ...19761254564
peptide utilization by pseudomonas putida and pseudomonas maltophilia.pseudomonas putida assimilates peptides and hydrolyses them with intracellular peptidases. amino acid auxotrophs (his, trp, thr or met) grew on a variety of di- and tripeptides up to twice as slowly as with free amino acids. pseudomonas putida has separate uptake systems for both dipeptides and oligopeptides (three or more residues). although the dipeptide system transported a variety of structurally diverse dipeptides it did not transport peptides having either unprotonatable n-terminal amino g ...19761255131
intracellular peptide hydrolysis by pseudomonas putida and pseudomonas maltophilia.amino acids liberated by peptidase hydrolysis of di- and oligopeptides by pseudomonas putida were measured by trinitrobenzenesulphonate assay and high voltage electrophoresis or paper chromatography followed by ninhydrin spray. intact bacteria or periplasmic contents released by lysozyme treatment did not hydrolyse peptides. subcellular fractionation showed that glycylmethionine peptidase activity was cytoplasmic. this enzyme had a km of 2 mm, and was stimulated fivefold by i mm-co2+. crude pept ...19761255132
constitutive synthesis of enzymes of the protocatechuate pathway and of the beta-ketoadipate uptake system in mutant strains of pseudomonas putida.mutant pseudomonas putida strains that produce constitutive levels of the beta-ketoadipate uptake system are selected by the sequential transfer of cultures between mineral growth media supplemented with the noninducing growth substrate succinate and growth media containing beta-ketoadipate as the sole carbon and energy source. the mutant strains also produce constitutively three catabolic enzymes that give rise to beta-ketoadipate from the metabolic precursor beta-carboxy-cis, cis-muconate, and ...19761262305
evidence for autogenous regulation of pseudomonas putida tryptophan synthase.studies of a trpa mutant constitutive for tryptophan synthase production support the hypothesis of autogenous regulation (r. f. goldberger, 1974; a. r. proctor and i. p. crawford, 1975) of the pseudomonas putida trpab loci.19761262309
the hydroxylation of p-cresol and its conversion to p-hydroxybenzaldehyde in pseudomonas putida. 19761267796
supra-operonic clustering of genes specifying glucose dissimilation in pseudomonas putida.the linkage arrangements of genes governing glucolysis in pseudomonas putida have been determined by transductional analysis. five genes (gdh, kgta, kgtb, edd and eda), comprising at least three operons, are contransducible with each other, but not with ggu (glucose and gluconate uptake) nor with genes of a known supra-operonic cluster of genes specifying enzymes of other dissimilatory pathways, nor with a biochemically uncharacterized his marker. it thus appears that p. putida may have more tha ...19761272248
[comparative study of naphthalene catabolism routes in 2 strains of pseudomonas putida]. 19761277993
multiple forms of rat liver cytochrome p-450. immunochemical evidence with antibody against cytochrome p-448.purified hepatic cytochrome p-448 from 3-methylcholanthrene-treated rats was used to produce antibody in rabbits. the cytochrome p-448 antibody (igg fraction) isolated from immune rabbit serum is quite specific and precipitates purified rat liver cytochrome p-448 at low antibody to protein ratios when assayed by the ouchterlony double diffusion technique. purified hepatic cytochrome p-450 from phenobarbital-treated rats cross-reacts poorly with the cytochrome p-448 antibody as do purified rabbit ...1976815258
isolation of plasmid deoxyribonucleic acid from pseudomonas putida.conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of pseudomonas putida containing degradative plasmids (cam, sal, oct, etc.) have been defined. these degradative plasmids could not be isolated by the usual procedure, whereas rp1, an r factor of the p group, present in the isogenic strain of p. putida, was isolated equally well by either the usual procedure or the modified procedure. characterization by electron microscopy of rp1 deoxy ...1976816778
superoxide dismutase from mycobacterium tuberculosis.1. a superoxide dismutase [ec 1.15.1.1] was purified about 275-fold with a yield of 34% from mycobacterium tuberculosis, strain h37ra (attenuated strain), grown on a sauton medium for two months. the purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis, and by analytical ultracentrifugation and sedimentation equilibrium studies. 2. the molecular weight of the enzyme was estimated to be approximately 88,000 by sedimentation equilibrium analysis. since the molecular weig ...1976828161
failure of complex supplementation of minimal cultures to elicit a shift-up response in pseudomonas putida.the addition of complex supplements (particularly amino acids) to cultures of pseudomonas putida growing on a good carbon source did not result in a substantial increase in the growth rate. amino acids entered the cells within 30 s of addition and reached significant internal pool concentrations. endogenous amino acid biosynthesis was quickly inhibited (about 75%), with a substantial sparing of the original carbon source. within 20 min of supplementation significant respiration of added amino ac ...1976932677
the p-cymene pathway in pseudomonas putida pl: isolation of a dihydrodiol accumulated by a mutant. 1976942434
metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in pseudomonas putida.two strains of pseudomonas putida isolated by enrichment cultures with orcinol as the sole source of carbon were both found to grow with resorcinol. data are presented which show that one strain (orc) catabolizes resorcinol by a metabolic pathway, genetically and mechanistically distinct from the orcinol pathway, via hydroxyquinol and ortho oxygenative cleavage to give maleylacetate, but that the other strain (o1) yields mutants that utilize resorcinol. one mutant strain, designated o1oc, was sh ...1976942589
crystallization and preliminary crystal data of iron-containing superoxide dismutase from pseudomonas ovalis.large single crystals of iron-containing superoxide dismutase from pseudomonas ovalis were prepared from 50% saturated ammonium sulfate solution, at ph 4.5, on gentle evaporation of the solvent at 4 degrees. the crystals were monoclinic, space group p2, with unit cell dimensions a = 81.9 a, b = 49.0 a, c = 61.0 a, and beta = 106 degrees. considerations of cell volume and protein molecular weight indicated 1 molecule of superoxide dismutase in the assymmetric unit, the smallest number reported s ...1976947910
[pseudomonas putida plasmid controlling the initial stages of naphthalene oxidation]. 1976949929
purification and properties of methioninase from pseudomonas ovalis. 1976955094
catechol oxygenases of pseudomonas putida mutant strains.investigation of a mutant strain of pseudomonas putida ncib 10015, strain psu-e1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. additional mutants of both wild-type and mutant strains psu-e1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. these findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate ...1976956121
involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by pseudomonas convexa.a microorganism capable of degrading dl-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as pseudomonas convexa. it was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. all the enzymes of the pathway were demonstrated in cell-free extracts. l-mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicot ...1976956122
current status of the sequence studies of the pseudomonas putida camphor hydroxylase system. 1976961531
enzyme evolution in a microbial community growing on the herbicide dalapon.a seven-membered microbial community capable of utilising the herbicide dalapon has been isolated by continuous-flow enrichment culture. the composition of this community has remained remarkably stable over thousands of hours in a dalapon-limited chemostat. during this period, however, one member of the community, pseudomonas putida, acquired the ability to grow on dalapon through the evolution of an extant dehalogenase.1976972691
purification and properties of l-4-hydroxymandelate oxidase from pseudomonas convexa.an inducible membrane-bound l-4-hydroxymandelate oxidase (decarboxylating) from pseudomonas convexa has been solubilized and partially purified. it catalyzes the conversion of l-4-hydroxymandelic acid to 4-hydroxybenzaldehyde in a single step with the stoichiometric consumption of o2 and liberation of co2. the enzyme is optimally active at ph 6.6 and at 55 degrees c. it requires fad and mn2+ for its activity. the membrane-bound enzyme is more stable than the solubilized and purified enzyme. afte ...1976976259
[microbial breakdown of caffeine (author's transl)].a bacterium, capable of growing aerobically with caffeine as its sole source of carbon and nitrogen, was isolated from soil and identified as pseudomonas putida sp. the breakdown of caffeine begins with stepwise demethylation, which leads via various n-methyl-purines to xanthine each step yielding formaldehyde. xanthine is then broken down via uric acid, allantoin, allantoic acid and further intermediates to urea and glyoxylic acid, which serves as the actual source of carbon.1976998047
aromatic-alcohol dehydrogenases from pseudomonas putida n.c.i.b. 9869. 19761001711
combined chromosomal and plasmid encoded control for the degradation of phenol in pseudomonas putida. 19761001897
creatine amidinohydrolase of pseudomonas putida: crystallization and some properties. 19761015832
the effect of a non-metabolizable analog on mandelate catabolism in pseudomonas putida.dl-2,3,4,5,6-pentafluoromandelic acid (pfm) specifically inhibits the growth of pseudomonas putida (atcc 12633) on medium containing mandelate as sole carbon and energy source by competitive inhibition of mandelate dehydrogenase. pfm is not metabolized and is neither an inducer of the mandelate catabolic enzymes nor an antagonist of induction. mutants resistant to the inhibitory effects of pfm (pfmr) were isolated; most prove to be superinducible, i.e. synthesize corrdinately the mandelate-speci ...19761015936
characterization of a benzoate permease mutant of pseudomonas putida.a spontaneous mutant of pseudomonas putida (prs 2017) has been isolated which is incapable of growth on benzoate, does not induce the enzymes of the catechol branch of the beta-ketoadipate pathway when grown in the presence of benzoate, cannot accumulate radioactively labeled benzoate, yet grows well with mandelate as sole source of carbon and energy. this strain apparently lacks a benzoate permease, which in the wild type shows a km of about 0.1 mm for benzoate, is inducible, and is not under t ...19761015938
the uptake of 2-ketogluconate by pseudomonas putida.the uptake of 2-ketogluconate is inducible in pseudomonas putida: 2-ketogluconate, glucose, gluconate, glycerol and glycerate were each good nutritional inducers of this ability. 2-ketogluconate uptake obeyed saturation kinetics (apparent km in 2-ketogluconate-grown cells was 0.4 mm). 2-ketogluconate was transported against a concentration gradient, apparently in an unchanged state, and the process required metabolic energy, all of which indicate an active transport system. a number of independe ...19761015939
regulation of the meta-cleavage of 4-hydroxyphenylacetic acid by pseudomonas putida. 19761027447
[generalized transduction of pseudomonas putida with a thermosensitive mutant of phage pf16h2]. 19761032694
regulation of arginine and pyrimidine biosynthesis in pseudomonas putida.repression of biosynthetic enzyme synthesis in pseudomonas putida is incomplete even when the bacteria are growing in a nutritionally complex environment. the synthesis of four of the enzymes of the arginine biosynthetic pathway (n-acetyl-alpha-glutamokinase/n-acetylglutamate-gamma-semialdehyde dehydrogenase, ornithine carbamoyltransferase and acetylornithine-delta-transaminase) could be repressed and derepressed, but the maximum difference observed between repressed and derepressed levels for a ...1976176312
plasmid-determined alcohol dehydrogenase activity in alkane-utilizing strains of pseudomonas putida.we have identified an alcohol dehydrogenase activity in pseudomonas putida strains carrying the cam-oct degradative plasmid that were grown on octane. the activity is nicotinamide adenine dinucleotide independent, sediments at 48,000 x g, and shows 20-fold greater activity with octanol rather than butanol as substrate. the enzyme is inducible by unoxidized alkane and is present only in strains that have the oct plasmid genes for alkane degradation with a wild-type alco locus. no analogous chromo ...1976177405
bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.cells of pseudomonas putida np, pseudomonas species (ncib 9816), and a nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (nad+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. similarly, cells of a strain of p. putida biotype a, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis ste ...197662750
[functional irreversibility of the anabolic ornithine carbamyltransferase in pseudomonas putida. kinetic analysis]. 197664201
immunological comparison of enzymes of the beta-ketoadipate pathway.beta-carboxy-cis,cis-muconate lactonizing enzyme and gamma-carboxymuconolactone decarboxylase catalyze sequential reactions in the beta-ketoadipate pathway; the subunit sizes of the enzymes from pseudomonas putida, biotype a, are 40 000 and 13 000, respectively. the cross reaction of antisera prepared against the enzymes was tested with the isofunctional enzymes formed by representatives of other bacterial species. despite the differences in the subunit sizes of the enzymes, the antisera reveale ...197665161
purification and characterization of methioninase from pseudomonas putida.methioninase of pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. the purified enzyme had an s20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. a break in the arrhenius plot was observed at 40 degrees and the activation energies below and above this temperature were 15.5 and 2.97 kcal per mole, respectively. 3. in addition to l-methionine, vari ...19768440
purification, crystallization, and some properties of creatine amidinohydrolase from pseudomonas putida.a method was developed for purification and crystallization of creatinase creatine amidinohydrolase, ec 3.5.3.3 from pseudomonas putida var. naraensis c-83. the purified preparation appeared homogeneous on disc electrophoresis and ultracentrifugation and had a molecular weight of 94,000. it was most active at ph 8 and stable between ph 6 and 8 for 24 hr at 37 degrees. sds-polyacrylamide gel electrophoresis indicated that the native enzyme was made up of two subunit monomers, the molecular weight ...19768443
purifications and properties of l-mandelate- 4-hydroxylase from pseudomonas convexa. 19769909
properties of iron-sulfur protein isolated from pseudomonas ovalis. 197610907
serum bactericidal activity in the horseshoe crab, limulus polyphemus.serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ...197611188
kinetics and properties of l-glutaminase and l-asparaginase activities of pseudomonas ovalis.pseudomonas ovalis produces l-glutaminase and l-asparaginase activities simultaneously upon induction by l-glutamine or l-asparagin in the growth medium. both activities are confined to the cell during active growth and are not released into the medium. the apparent km values are 1.4 x 10(-2) m and 6 x 10(-3) m for l-glutamine and l-asparagine substrates, respectively. induction of both activities is substantially favoured in media with initial ph values higher than 7. in buffered yeast extract ...197613588
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