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enzymatic production of l-citrulline by pseudomonas putida.to develop an efficient method for the production of l-citrulline, optimum conditions for the conversion of l-arginine to l-citrulline by microbial l-arginine deiminase and for production of the enzyme were studied. a number of micro-organisms were screened to test their ability to form and accumulate l-citrulline from l-arginine. pseudomonas putida was selected as the best organism. with this organism, enzyme activity as high as 9.20 units per ml could be produced by a shaking culture at 30 c i ...19715137589
2'-o-methyladenosine 5'-triphosphate. a substrate for deoxyribonucleic acid dependent ribonucleic acid polymerase of pseudomonas putida. 19714935104
pseudomonas putida tryptophan synthetase: partial sequence of the subunit.there is 50% identity in the sequences of the first 50 residues of the alpha chains of escherichia coli and pseudomonas putida. no deletions or additions of residues are found in this region, except for the n-terminal methionine residue which is missing in the polypeptide isolated from p. putida. most of the residues which differ are chemically dissimilar, and half of them are specified by codons which differ by more than a single base. the two residues known by mutational analysis to be essenti ...19714941557
pseudomonas putida tryptophan synthetase.the two protein components of pseudomonas putida tryptophan synthetase have been purified to homogeneity. although there is general similarity between the pseudomonas enzyme and that of the enteric bacteria, many differences were found. components from escherichia coli and p. putida do not stimulate each other enzymatically, and the enzymes differ in their response to monovalent cations. serine deamination occurs best with the intact enzyme of p. putida, not with the beta(2) subunit alone as in ...19714941565
deoxyribonucleic acid-dependent ribonucleic acid polymerase of pseudomonas putida. 19715101769
apo- and reconstituted holoenzymes of metapyrocatechase from pseudomonas putida. 19715127383
the metabolism of biphenyl by pseudomonas putida. 19715127867
induction of separate catabolic pathways for l- and d-lysine in pseudomonas putida. 19715128165
some studies on the purified 2-furoyl-coenzyme a hydroxylase from pseudomonas putida f2. 19715144719
the role of phosphoglycerate kinase in the metabolism of pseudomonas putida. 197111945789
crystallization of l-arginine deiminase from pseudomonas putida. 197111946203
photoactivation of urocanase in pseudomonas putida: possible conformational change during activation. 19725051931
inducible uptake system for -carboxy-cis, cis-muconate in a permeability mutant of pseudomonas putida.a procedure for the large-scale enzymatic synthesis of beta-carboxymuconate is described. when used as a growth substrate, beta-carboxymuconate selected for mutant strains of pseudomonas putida that were permeable to polycarboxylic acid intermediates of the beta-ketoadipate pathway. one mutant organism, strain prs2110, was investigated in detail. it differed from the parental strain in that it possessed a beta-carboxymuconate uptake system that was formed when the compound was supplied exogenous ...19725053469
template activity of 2'-o-methylpolyribonucleotides with pseudomonas putida dna-dependent rna polymerase. 19725012160
release of the sigma subunit of pseudomonas putida deoxyribonucleic acid dependent ribonucleic acid polymerase. 19725013820
metabolism of basic amino acids in pseudomonas putida. transport of lysine, ornithine, and arginine. 19725019949
photoactivation of urocanase in pseudomonas putida: action spectrum. 19725021838
regulation of valine catabolism in pseudomonas putida.the activities of six enzymes which take part in the oxidation of valine by pseudomonas putida were measured under various conditions of growth. the formation of four of the six enzymes was induced by growth on d- or l-valine: d-amino acid dehydrogenase, branched-chain keto acid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, and methylmalonate semialdehyde dehydrogenase. branched-chain amino acid transaminase and isobutyryl-coa dehydrogenase were synthesized constitutively. d-amino acid dehy ...19725030618
catabolism of pipecolate to glutamate in pseudomonas putida. 19725033403
metabolism of basic amino acids in pseudomonas putida. -guanidinobutyrate amidinohydrolase. 19725043851
carbon monoxide binding by pseudomonas putida cytochrome p-450. 19725045928
regulation of pathways degrading aromatic substrates in pseudomonas putida. enzymic response to binary mixtures of substrates.1. induction constants (k(ind)) and repression constants (k(rep)), which are a measure of the affinity of the inducers or repressors for the induction systems, were measured for mandelate, benzoate and p-hydroxybenzoate in pseudomonas putida. 2. from these results, the enzymic response of the organism to media containing pairs of these substrates was predicted. nitrogen-limited chemostats, operated at high growth rates, were used to investigate these predictions in cells grown first on one aroma ...19725073241
evidence for induced synthesis of an active transport factor for mandelate in pseudomonas putida.1. at low concentrations of mandelate there is a lag before induction of the mandelate regulon begins at a sub-maximum rate. cells preinduced with a saturating concentration of inducer do not exhibit this lag when they are transferred to sub-maximum inducer concentrations and are able to maintain a high rate of induction under these conditions. 2. chloramphenicol was used to show that a protein is synthesized during the lag period that is probably responsible for the maintenance effects observed ...19725073242
genetic control of enzyme induction in the -ketoadipate pathway of pseudomonas putida: two-point crosses with a regulatory mutant strain.several mutant strains of pseudomonas putida, selected on the basis of their inability to grow at the expense of benzoate, have been shown to be unable to form inducibly both muconate lactonizing enzyme and muconolactone isomerase. a secondary mutant strain derived from one of these pleiotropically negative strains forms these two enzymes and, in addition, catechol oxygenase in the absence of inducer. this constitutive mutant strain was used as a donor in transductionally mediated two-point cros ...19725058453
pseudomonas putida cytochrome p-450: characterization of an oxygenated form of the hemoprotein. 19724335959
the metabolic divergence in the meta cleavage of catechols by pseudomonas putida ncib 10015. physiological significance and evolutionary implications. 19724342908
a heat-stable nicotinamide-adenine dinucleotide glycohydrolase from pseudomonas putida kb1. partial purification and some properties of the enzyme and an inhibitory protein.a thermostable nad(p)(+) glycohydrolase (ec 3.2.2.6) detected in cell-free extracts of pseudomonas putida kb1 was purified to a single component on polyacrylamide-gel electrophoresis. a heat-labile inhibitor of the enzyme was also partially purified. enzyme free of inhibitor is present in culture supernatants. after an ultrasonic treatment enzyme-inhibitor complex and excess of inhibitor are present in both the cell-debris and soluble fractions. the general properties of the enzyme and inhibitor ...19724345853
the oxidation of nicotinic acid by pseudomonas ovalis chester. the terminal oxidase.in cell-free extracts of pseudomonas ovalis nicotinic acid oxidase is confined to the wallmembrane fraction. it is associated with an electron-transport chain comprising b- and c-type cytochromes only, differing proportions of which are reduced by nicotinate and nadh. co difference-spectra show two co-binding pigments, cytochrome o (absorption maximum at 417nm) and another component absorbing maximally at 425nm. cytochrome o is not reduced by nadh or by succinate but is by nicotinate, which can ...19724349118
metabolism of gallic acid and syringic acid by pseudomonas putida. 19724342601
the metabolism of benzoate and methylbenzoates via the meta-cleavage pathway by pseudomonas arvilla mt-2. 19724342906
urocanase of pseudomonas putida. subunit structure and origin of enzyme-bound -ketobutyrate. 19724404600
purification and properties of 2-furoyl-coenzyme a hydroxylase from pseudomonas putida f2.1. 2-furoyl-coa hydroxylase of pseudomonas putida f2 has been purified 60-fold by a combination of (nh(4))(2)so(4) fractionation, deae-cellulose chromatography and agarose chromatography. 2. the purified enzyme catalyses the formation of 5-hydroxy-2-furoyl-coa, which tautomerizes to form 5-oxo-delta(2)-dihydro-2-furoyl-coa. 3. the enzyme has a requirement for an electron acceptor that can be satisfied by a membrane preparation from 2-furoate-grown ps. putida f2 or by artificial electron acceptor ...19724655411
the divergent meta-cleavage pathway for the metabolism of benzoate, 3-methylbenzoate and 4-methylbenzoate by pseudomonas arvilla mt-2. 19724634855
isolation, identification, and characterization of a lipoate-degrading pseudomonad and of a lipoate catabolite.a strain of bacteria that can degrade lipoic acid was isolated from soil. the bacterium, adapted to use 0.4% dl-lipoate as the sole organic substrate to supply carbon, sulfur, and energy, was identified morphologically and physiologically as a strain of pseudomonas putida. degradation of 1,6-(14)c-lipoic acid, synthesized from 1,6-(14)c-adipic acid, was evidenced by: (i) loss of approximately 50% of the total radioactivity from the medium after bacterial growth; (ii) appearance of (14)c-degradat ...19724565525
genetic control of enzyme induction in the -ketoadipate pathway of pseudomonas putida: deletion mapping of cat mutations.a number of spontaneous mutant strains of pseudomonas putida, obtained by repeated selection for inability to grow with cis,cis-muconate, have been shown to carry deletions in catb, the structural gene for muconate lactonizing enzyme. these strains have been employed for deletion mapping of the genetic region containing catb and catc (the structural gene for muconolactone isomerase, the synthesis of which is coordinate with that of muconate lactonizing enzyme). all deletions that overlap mutant ...19724621687
regulation of a catabolic pathway. lysine degradation in pseudomonas putida. 19724640470
camphor binding by pseudomonas putida cytochrome p-450. kinetics and thermodynamics of the reaction. 19724655251
proton magnetic resonance reveals high-spin iron (ii) in ferrous cytochrome p450 cam from pseudomonas putida.high-resolution proton nuclear magnetic resonance spectra were studied in d(+)-camphor-saturated solutions of ferric and ferrous cytochrome p450(cam) from pseudomonas putida, and of the cyanide complex of ferric p450(cam). in all these compounds several hyperfineshifted resonances of the heme group could be detected. in the ferrous protein, these resonance lines, which exhibit a curie-type temperature dependence in the range of 5-28 degrees , indicate the presence of high-spin iron (ii). it is s ...19724506075
genetic basis of the biodegradation of salicylate in pseudomonas.the genetic basis of the biodegradation of salicylate in pseudomonas putida r1 has been studied. this strain utilizes the meta pathway for oxidizing salicylate through formation of catechol and 2-hydroxymuconic semialdehyde. the enzymes of the meta pathway are induced by salicylate but not by catechol, and the genes specifying these enzymes are clustered. the gene cluster can be eliminated from some salicylate-positive cells by treatment with mitomycin c and appears to exist inside the cell as a ...19724628746
kinetics of deoxyribonucleic acid destruction and synthesis during growth of bdellovibrio bacteriovorus strain 109d on pseudomonas putida and escherichia coli.during the growth of bdellovibrio bacteriovorus on pseudomonas putida or escherichia coli in either 10(-3)m tris(hydroxymethyl)aminomethane or in dilute nutrient broth, the host deoxyribonucleic acid (dna) was rapidly degraded, and by 30 to 60 min after the initiation of the bdellovibrio development cycle essentially all host dna became nonbandable in cscl gradients. at this stage the host dna degradation products were nondiffusable, and there was no appreciable pool of low-molecular-weight (col ...19724559819
stereospecific enzymes in the degradation of aromatic compounds by pseudomonas putida.two reactions in the catabolism of catechol by meta-fission, namely, hydration of 2-oxopent-4-enoate (vinylpyruvate) and aldol fission of the product, are catalyzed by stereospecific enzymes. the absolute configuration of this hydration product was shown to be l(s)-4-hydroxy-2-oxopentanoate. vinylpyruvate hydratase, purified almost to homogeneity, had a molecular weight of about 287,000 and was dissociated in sodium dodecyl sulfate, without prior treatment with mercaptoethanol, into a species wi ...19734690969
incidence and identification of pseudomonas fluorescens and pseudomonas putida in the clinical laboratory.strains of pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. a rapid nitrate test was found to be useful in distinguishing p. aeruginosa (positive) from p. fluorescens and p. putida (both negative). a shortened gelatin test differentiated p. fluorescens (positive) from p. putida (negative). p. fluorescens and p. putida were very sensitive to low levels of kanamycin and resistant to carbenicillin, a pattern just the oppo ...19734631431
camphor plasmid-mediated chromosomal transfer in pseudomonas putida.camphor-utilizing strains of pseudomonas putida have been shown to carry the genetic information required for camphor degradation on a plasmid. the plasmid-carrying strains can serve as donors of both plasmid-borne and chromosomal genes. as recipients, plasmid-deleted strains are much superior to those carrying the camphor pathway genes. the transfer frequency of chromosomal, but not plasmid-borne, genes is markedly enhanced if the donor cells are irradiated with ultraviolet light followed by 3- ...19734745436
growth of pseudomonas eisenbergii in activated sludges of water-treatment plants. 19734802488
the clustering on the pseudomonas putida chromosome of genes specifying dissimilatory functions. 19734807194
benzoate metabolism in pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways.benzoate-grown cells of pseudomonas putida(arvilla) mt-2 contain both metapyrocatechase and pyrocatechase activities, although the former activity is much higher than that of the latter. a spontaneous mutant deficient in metapyrocatechase and 2-hydroxymuconic semialdehyde hydrolyase, the first two enzymes in the meta-cleavage pathway of the ring of catechol, has been isolated from this strain. this mutant grows well on a minimal medium containing benzoate as a sole carbon source and has the high ...19734717515
microbial metabolism of amino alcohols. 1-aminopropan-2-ol and ethanolamine metabolism via propionaldehyde and acetaldehyde in a species of pseudomonas.1. growth and manometric experiments showed that a pseudomonas sp. p6 (n.c.i.b. 10431), formerly known as achromobacter sp. p6, was capable of growth on both stereoisomers of 1-aminopropan-2-ol, and supported the hypothesis that assimilation involved metabolism to propionaldehyde, propionate and possibly 2-hydroxyglutarate. a number of alternative intermediary metabolites were ruled out. 2. accumulation of propionaldehyde from 1-aminopropan-2-ol by intact cells occurred only during active growth ...19734723219
glucolysis in pseudomonas putida: physiological role of alternative routes from the analysis of defective mutants.a number of mutants in which glucolysis is impaired have been isolated from pseudomonas putida. the study of their behavior shows that this organism possesses a single glucolytic pathway with physiological significance. the first step of the pathway consists in the oxidation of glucose into gluconate. two proteins with glucose dehydrogenase activity appear to exist in p. putida but the reasons for this duplicity are not clear. the process continues with the formation of 2-ketogluconate which is ...19734745434
initial reactions in the oxidation of ethylbenzene by pseudomonas putida. 19734699984
pseudomonas putida and septic arthritis. 19734713580
common enzymes of branched-chain amino acid catabolism in pseudomonas putida.two types of pseudomonas putida ppg2 mutants which were unable to degrade branched-chain amino acids were isolated after mutagenesis and selection for ability to grow on succinate, but not valine, as a sole source of carbon. these isolates were characterized by growth on the three branched-chain amino acids (valine, isoleucine, and leucine), on the corresponding branched-chain keto acids (2-ketoisovalerate, 2-keto-3-methylvalerate, and 2-ketoisocaproate), and on other selected intermediates as c ...19734352175
cytochrome c linked nicotinic acid hydroxylase in pseudomonas ovalis chester. 19734354821
genetic fusion of incompatible plasmids in pseudomonas.the genes specifying enzymes responsible for the degradation of camphor and octane occur on transmissible plasmids in pseudomonas putida strain ppg1 and p. oleovorans. since the presence of the plasmids is vital for the oxidative metabolism of camphor or octane (by the cells) in the absence of other carbon sources, such naturally occurring, energy-generating plasmids have been designated as degradative plasmids. the two degradative plasmids, cam and oct, are incompatible with each other and cann ...19734515925
[activation of fatty acids by an enzyme system extracted from pseudomonas putida]. 19734793588
anthranilate synthase from pseudomonas putida. purification and properties of a two-component enzyme. 19734692828
transmissible plasmid coding early enzymes of naphthalene oxidation in pseudomonas putida.the capacity of pseudomonas putida ppg7 (atcc 17,485) to grow on naphthalene, phenotype nah(+), is lost spontaneously, and the frequency is increased by treatment with mitomycin c. the nah(+) growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. after mutagenesis, strains can be selected with increased donor capacity in conjugation. clones which use naphthalene grow on s ...19734712575
regulation of the glucolytic enzymes in pseudomonas putida. 19734764724
transformation of o-toluate in pseudomonas putida isolate 1065 and rhizopus japonicus atcc 24794. 19734775413
the effects of trypsin on the membrane-bound nicotinic acid oxidase in pseudomonas ovalis chester. 19734359145
genetic control of the histidine dissimilatory pathway in pseudomonas putida. 19734405673
interactions of substrates with a purified 4-methoxybenzoate monooxygenase system (o-demethylating) from pseudomonas putida. 19734351526
a transmissible plasmid controlling camphor oxidation in pseudomonas putida.earlier papers demonstrated an extensive genetic exchange among fluorescent pseudomonads; this one documents for genes specifying enzymes of peripheral dissimilation an extrachromosomal array, segregation, and frequent interstrain transfer. an hypothesis is presented of a general mechanism for the formation and maintenance of metabolic diversity. the example used, the path of oxidative cleavage of the carbocyclic rings of the bicyclic monoterpene d- and l-camphor, terminates in acetate release a ...19734351810
absolute stereochemistry of the (+)-cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene produced from toluene by pseudomonas putida. 19734710064
relationships among enzymes of the beta-ketoadipate pathway. i. properties of cis,cis-muconate-lactonizing enzyme and muconolactone isomerase from pseudomonas putida. 19734199894
relationships among enzymes of the beta-ketoadipate pathway. ii. properties of crystalline beta-carboxy-cis,cis-muconate-lactonizing enzyme from pseudomonas putida. 19734199895
relationships among enzymes of the beta-ketoadipate pathway. 3. properties of crystalline gamma-carboxymuconolactone decarboxylase from pseudomonas putida. 19734199896
metabolism of phenol and cresols by mutants of pseudomonas putida.mutant strains of pseudomonas putida strain u have been obtained which are deficient in enzymes of the degradative pathways of phenol and cresols. mutant strains deficient in catechol 2, 3-oxygenase accumulated the appropriate catechol derivative from cresols. a mutant strain which would not grow on either phenol or a cresol was shown to be deficient in both 2-hydroxymuconic semialdehyde hydrolase and a nicotinamide adenine dinucleotide, oxidized form, (nad(+))-dependent aldehyde dehydrogenase. ...19734347965
mandelic acid-4-hydroxylase, a new inducible enzyme from pseudomonas convexa. 19734145649
the degradation of l-histidine, imidazolyl-l-lactate and imidazolylpropionate by pseudomonas testosteroni.1. imidazol-5-ylpropionate and imidazol-5-yl-lactate are degraded by pseudomonas testosteroni via inducible pathways. 2. growth on either compound as the sole source of carbon results in the induction of the enzymes for histidine catabolism. 3. the pathway of histidine degradation in this organism, a non-fluorescent pseudomonad, is shown to be the same as that operating in pseudomonas fluorescens and pseudomonas putida. it consists of the successive formation of urocanate, imidazol-4-on-5-ylprop ...19734146796
pseudomonas putida cytochrome p-450: ligands of the substrate-free and substrate-bound states of the ferric hemoprotein. 19734149376
metabolism of biphenyl. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate: the meta-cleavage product from 2,3-dihydroxybiphenyl by pseudomonas putida.1. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid was isolated and identified from washed suspensions of pseudomonas putida incubated in the presence of 2,3-dihydroxybiphenyl. 2. benzoic acid was isolated from reaction mixtures of crude cell-free extracts incubated with 2,3-dihydroxybiphenyl. 3. the presence in the same reaction mixtures of either 4-hydroxy-2-oxovalerate or 2-hydroxypenta-2,4-dienoate was suggested by mass spectrometry. 4. the degradative pathway of biphenyl is discussed.19734762751
a 4-methoxybenzoate monooxygenase system from pseudomonas putida. circular dichroism studies on the iron--sulfur protein. 19744850360
bacterial metabolism of para- and meta-xylene: oxidation of the aromatic ring.pseudomonas putida 39/d oxidized p-xylene to cis-3,6-dimethyl-3,5-cyclohexadiene-1,2-diol (cis-p-xylene dihydrodiol). the latter compound was isolated in crystalline form and its physical properties were determined. the cis configuration of the hydroxyl groups in the oxidation product was inferred from its ability to form an isopropylidene derivative with 2,2-dimethoxypropane. acid treatment of cis-p-xylene dihydrodiol resulted in the formation of 2,5-dimethylphenol. a partially purified prepara ...19744850728
the amino acid sequence of putidaredoxin, an iron-sulfur protein from pseudomonas putida. 19744833743
involvement of threonine dehydratase in biosynthesis of the alpha-ketobutyrate prosthetic group of urocanase.seventeen mutants of pseudomonas putida that were unable to grow on threonine as nitrogen source owing to a lack of threonine dehydratase were isolated, and all were found to be unable to synthesize active urocanase. spontaneous revertants selected for urocanase production concomitantly regained threonine dehydratase. mutants that were unable to utilize urocanate as carbon source were also isolated, and these were defective in urocanase formation but were normal in threonine dehydratase levels. ...19744154935
effect of temperature on histidine ammonia-lyase from a psychrophile, pseudomonas putida.pseudomonas putida was able to grow at 0 c in a complex medium containing l-histidine and to synthesize histidine ammonia-lyase and urocanase. the activity of the former enzyme was assessed between -10 and 60 c in cells and in cell extracts. activity was maximal from 20 to 35 c. below 20 c, activity decreased with temperature but, significantly, the enzyme exhibited 30% of its maximal activity at 1.5 c. the temperature response was similar in both intact cells and cell extracts, which indicated ...19744152044
amino-terminal sequence of the tryptophan synthetase alpha chain of bacillus subtilis.the sequence of the 46 nh(2)-terminal residues of the tryptophan synthetase alpha chain of bacillus subtilis was determined and compared with the corresponding sequences of escherichia coli, shigella dysenteriae, salmonella typhimurium, aerobacter aerogenes, serratia marcescens, and pseudomonas putida. a deletion of six residues was found at the nh(2)-terminal end of the alpha chain of b. subtilis.19744206869
relationships among enzymes of the beta-ketoadipate pathway. iv. muconolactone isomerase from acinetobacter calcoaceticus and pseudomonas putida. 19744215816
effect of temperature on urocanase from a psychrophile, pseudomonas putida. 19744150614
d- and l-isoleucine metabolism and regulation of their pathways in pseudomonas putida.pseudomonas putida oxidized isoleucine to acetyl-coenzyme a (coa) and propionyl-coa by a pathway which involved deamination of d-isoleucine by oxidation and l-isoleucine by transamination, oxidative decarboxylation, and beta oxidation at the ethyl side chain. at least three separate inductive events were required to form all of the enzymes of the pathway: d-amino acid dehydrogenase was induced during growth in the presence of d-isoleucine; branched-chain keto dehydrogenase was induced during gro ...19744150713
regulation of leucine catabolism in pseudomonas putida.the generation time of pseudomonas putida with l-leucine was 20 h in synthetic media but only 3 h with d-leucine. slow growth in the presence of l-leucine was partially overcome by addition of 0.1 mm amounts of either d-valine, l-valine, or 2-ketoisovalerate. the activities of five enzymes which take part in the oxidation of leucine by p. putida were measured under various conditions of growth. four enzymes were induced by growth with dl-leucine as sole source of carbon: d-amino acid dehydrogena ...19744150714
purification and propeties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of pseudomonas putida.cells of pseudomonas putida, after growth with naphthalene as sole source of carbon and energy, contain an enzyme that oxidizes (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. the purified enzyme has a molecular weight of 102,000 and apparently consists of four 25,500 molecular weight subunits. the enzyme is specific for nicotinamide adenine dinucleotide as an electron acceptor and also oxidizes several other cis-dihydrodiols. however, no enzymatic activity was ob ...19744369091
a mutant of pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. 19744371622
structural resemblance of cytochrome p-450 isolated from pseudomonas putida and from rabbit liver microsomes. 19744418751
pseudomonas putida mutants defective in the metabolism of the products of meta fission of catechol and its methyl analogues.a selection procedure is described which was used to isolate mutants of pseudomonas putida strain u in the following enzymes of the meta-fission pathway of phenol and cresols: hydrolase, tautomerase, and decarboxylase. strains deficient in the hydrolase are unable to use either o- or m-cresol as a sole carbon source and were shown to accumulate 2-hydroxy-6-keto-2,4-heptadienoate when incubated in the presence of o- or m-cresol. when 2-hydroxymuconic semialdehyde (plus nicotinamide adenine dinucl ...19744418942
[effects of soil constituents on the biological activity of "pseudomonas putida" (author's transl)]. 19744419273
pseudomonas putida cytochrome p-450. binding of a spin-labeled analog of the inhibitor metyrapone. 19744364068
bacterial degradation of 4-hydroxyphenylacetic acid and homoprotocatechuic acid.a species of acinetobacter and two strains of pseudomonas putida when grown with 4-hydroxyphenylacetic acid gave cell extracts that converted 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) into carbon dioxide, pyruvate, and succinate. the sequence of enzyme-catalyzed steps was as follows: ring-fission by a 2,3-dioxygenase, nicotinamide adenine dinucleotide-dependent dehydrogenation, decarboxylation, hydration, aldol fission, and oxidation of succinic semialdehyde. two new metabolites, ...19744420192
transmissible plasmid coding for the degradation of benzoate and m-toluate in pseudomonas arvilla mt-2. 19744424218
fusion and compatibility of camphor and octane plasmids in pseudomonas.the octane (oct) plasmid in pseudomonas putida derived from the omega-hydroxylase-carrying strain of coon and coworkers is transferable to the camphor (cam) plasmid-bearing strain by conjugation or by transduction. while the majority of the cam (+)oct(+) exconjugants segregate cam(+) or oct(+) cells, exconjugants with stable cam (+)oct(+) phenotype (cam-oct) can be detected at a low frequency. the transductants are all of the cam-oct phenotype. in the stable cam (+)oct(+) strains, the oct plasmi ...19744527812
a role of the putidaredoxin cooh-terminus in p-450cam (cytochrome m) hydroxylations.methylene hydroxylation by cytochrome p-450(cam) (cytochrome m) can be resolved into four distinct steps: substrate addition, m(o) --> m(os); reduction, m(os) --> m(rs); dioxygen addition, m(rs) --> m(o2) (rs); followed by a second putidaredoxin (pseudomonas putida ferredoxin)-mediated reduction and product formation. the isolated ferrous oxy-substrate complex exhibits first-order decay kinetics with the relatively slow rate constant of k [unk] 0.01 sec(-1), at 25 degrees , without product relea ...19744530269
dissociation and interaction of individual components of a degradative plasmid aggregate in pseudomonas.the transfer of the oct plasmid from pseudomonas oleovorans to pseudomonas putida strain ppgl results in the acquisition of three independent replicons: oct, factor k, and the mer plasmid. oct is a nontransmissible plasmid harboring genes that code for the enzymes responsible for the degradation of n-octane. factor k is a transfer plasmid capable of mobilizing oct as well as chromosomal genes but incapable of enhancing transfer frequencies of other transmissible plasmids such as cam, sal, or rp- ...19744530312
metabolism of benzoate and the methylbenzoates by pseudomonas putida (arvilla) mt-2: evidence for the existence of a tol plasmid.mutant strains of pseudomonas putida (arvilla) mt-2 which have lost the ability to grow at the expense of m- or p-toluate (methylbenzoate) but retain the ability to grow with benzoate arise spontaneously during growth on benzoate; this genetic loss occurs to a lesser extent during growth on nonaromatic carbon sources in the presence of mitomycin c. the mutants have totally lost the activity of the enzymes of the divergent meta pathway with the possible exception of 2-oxopent-4-enoate hydratase a ...19744418209
effect of carbamates and anilines on pseudomonas putida and soil microbiol activity. 19744418476
d-lysine catabolic pathway in pseudomonas putida: interrelations with l-lysine catabolism.the isolation of several mutant strains blocked in l-lysine degradation has permitted an assessment of the physiological significance of enzymatic reactions related to lysine metabolism in pseudomonas putida. additional studies with intact cells involved labeling of metabolic intermediates from radioactive l- or d-lysine, and patterns of enzyme induction in both wild-type and mutant strains. these studies lead to the conclusions that from l-lysine, the obligatory pathway is via delta-aminovalera ...19744359655
[evaluation of culture media used in the differentiation of pseudomonas aeruginosa, pseudomonas putida, and pseudomonas fluorescens, isolated from clinical human material]. 19744549846
molecular properties of cis,cis-muconate cycloisomerase from pseudomonas putida. 19744449126
metabolism of biphenyl. structure and physicochemical properties of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, the meta-cleavage product from 2,3-dihydroxybiphenyl by pseudomonas putida.the structure of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid for the meta-cleavage product of 2,3-dihydroxybiphenyl by a pseudomonas putida strain was demonstrated on the basis of its chemical and physicochemical properties and those of its derivatives.19744462560
production of l-asparaginase by pseudomonas ovalis. 19744480497
magnetic circular dichroism studies. xxv. a preliminary investigation of microsomal cytochromes.the application of magnetic circular dichroism as an optical probe for simultaneous identification and determination of at least two microsomal cytochromes is demonstrated. the assignments of the bands in the spectra of microsomal suspensions are made from the spectra of soluble preparations of cytochrome p-450 obtained from pseudomonas putida and of cytochrome b(5) obtained from rat livers.19744521811
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