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a comparative study of the nah and tol catabolic plasmids in pseudomonas putida.a comparative study of the nah and tol catabolic plasmids was carried out to provide information for future genetic manipulation experiments involving these two plasmids. the plasmids were studied in a strain of p. putida and its mutant derivatives. the nah and tol plasmids were found to be incompatible. under the conditions used in these experiments the tol plasmid transferred into some strains into which nah was unable to transfer. the use of mutants to remove certain catabolic activities enco ...1977603460
in vitro inhibition of human peripheral blood lymphocyte transformation by an extract of pseudomonas putida.an extract prepared from a psychrophilic strain of pseudomonas putida was found to cause a dose-dependent inhibition of [h3]tdr incorporation into human peripheral blood lymphocytes stimulated with pha, cona, pwm, or in a mixed lymphocyte reaction. the inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. the extract was most effective when added prior to ...1977608686
microbiological transformations of terpenes: part xxiii--fermentation of geraniol, nerol & limonene by a soil pseudomonad, pseudomonas incognita (linalool strain). 1977612543
metabolism of d- and l-lactate by pseudomonas putida.pseudomonas putida grew at the same rate with the same molar growth yield on d-, l, or dl-lactate as the sole source of carbon for growth. d- and l- lactate were utilized simultaneously and at the same rate when the organism was grown on dl-lactate (ratio of d isomer to l isomer of 1:1). growth on either isomer alone, or in combination, caused the induction of both a d-lactate, and an l-lactate dehydrogenase. both enzymes were particulate and used dichlorophenolindophenol, or oxygen, but not na ...1977614007
isolation of mutants with altered metabolic control of the nah plasmid-encoded catechol meta-cleavage pathway.two types of mutants which displayed altered regulation of the nah catabolic plasmid-encoded catechol meta-cleavage pathway were isolated in pseudomonas putida. altered metabolic control was indicated by assay of catechol 2,3-dioxygenase. in one type of mutant the catechol 2,3-dioxygenase was synthesized constitutively. in the other type the range of carbon sources which induce the catechol 2,3-dioxygenase was increased.1977614009
microbiological transformations of terpenes: part xxiv--pathways of degradation of linalool, geraniol, nerol & limonene by pseudomonas incognita (linalool strain). 1977615106
changes in cytochrome content and electron transport patterns in pseudomonas putida as a function of growth phase.optical absorbance difference spectra of membrane vesicles prepared from aerobically grown pseudomonas putida indicated that, when harvested in logarithmic phase, the cells contained one c-type cytochrome and two or three b-type cytochromes, one of which was cytochrome o. as the cells grew into stationary phase and the oxygen concentration of the medium dropped to essentially zero, an additional component believed to be cytochrome d was produced. both the o- and d-type cytochromes might function ...1978618838
phosphonate utilization by bacteria.bacteria able to use at least one of 13 ionic alkylphosphonates of o-alkyl or o,o-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. four of these isolates used 2-aminoethylphosphonic acid (aep) as a sole carbon, nitrogen, and phosphorus source. none of the other phosphonates served as a carbon source for the organisms. one isolate, identified as pseudomonas putida, grew with aep as its sole carbon, nitrogen, and phosphorus source and released nearly all of the o ...1978618850
[characteristics of pseudomonas putida plasmid dnas].physico-chemical characteristics of plasmid dnas isoalted from pseudomonas putida g7 were studied as well as the behavior of these dnas in th eourse of chromatography on columns with sepharose 4b and kieselguhr with methylated albumin (mac). this strain was found to contain several plasmid dnas having molecular weights of 33-36x10(6), 15-18x10(6), and 3-5x10(6) dalton. the plasmid dnas of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is c ...1978651695
mutation to increased resistance to phenol in pseudomonas putida. 1978656570
regulation of the degradative pathway enzymes coded for by the tol plasmid (pwwo) from pseudomonas putida mt-2.pseudomonas putida mt-2 carries a plasmid (tol, pwwo) which codes for a single set of enzymes responsible for the catabolism of toluene and m- and p-xylene to central metabolites by way of benzoate and m- and p-toluate, respectively, and subsequently by a meta cleavage pathway. characterization of strains with mutations in structural genes of this pathway demonstrates that the inducers of the enzymes responsible for further degradation of m-toluate include m-xylene, m-methylbenzyl alcohol, and m ...1978659369
anthranilate synthetase component ii from pseudomonas putida. covalent structure and identification of the cysteine residue involved in catalysis.the complete amino acid sequence of carboxamidomethylated anthranilate synthetase component ii (as ii) from pseudomonas putida has been determined by analysis of cyanogen bromide fragments, tryptic peptides from the citraconylated protein, and by analysis of subdigests of these peptides. as ii is a single polypeptide chain of 197 residues having a calculated molecular weight of 21,684. previous studies (goto, y., keim, p. s., zalkin, h., and heinrikson, r. l. (1976) j. biol. chem, 251, 941-949) ...1978659439
[naphthalene oxidation by a pseudomonas putida strain carrying a mutant plasmid].naphthalene oxidation by a parent and a mutant strain of pseudomonas putida was studied. the parent strain contained a plasmid npl-1 which controlled oxidation of naphthalene to salicylic acid and was capable of oxidizing salicylate. the mutant strain did not oxidize salicylate because of a mutation in salicylate hydroxylase; it contained also a mutant plasmid npl-41 which determined constitutive synthesis of naphthalene oxygenase. salicylic acid which accumulated as a product of naphthalene cat ...1978661635
transformation of pseudomonas putida with chromosomal dna. 1978666840
magnetic circular dichroism of pseudomonas putida cytochrome p-450 in near infrared region.magnetic circular dichroism spectra of oxidized, reduced and carbonmonoxy reduced forms of cytochrome p-450 from d-camphor grown pseudomonas putida (p-450cam) were studied in the near infrared region (650 to 1200 nm) at various temperatures in the presence of d-camphor. oxidized p-450cam with camphor exhibited positive (+) and negative (-) magnetic cd bands at 825 and 970 nm, respectively, and both of them were assigned to faraday b terms. the magnetic cd spectrum of reduced p-450cam in the pres ...1978667105
nic, a conjugative nicotine-nicotinate degradative plasmid in pseudomonas convexa.the plasmid nature of genes specifying degradation of nicotine and nicotinate in pseudomas convexa strain 1 (pc1) is indicated by mitomycin curing and conjugational transfer to other strains. the nic plasmid appears to be compatible with other metabolic plasmids in pseudomonas putida.1978670150
the role of enoyl-coa hydratase in the metabolism of isoleucine by pseudomonas putida.the purpose of the present study was to determine if the enoyl coenzyme a hydratase formed by pseudomonas putida during growth on isoleucine was a unique enzyme specific for isoleucine metabolism. the highest levels of the hydratase were formed during growth on isoleucine intermediates and the lowest levels during growth on glutamate and glucose. data from growth experiments revealed that 2-methyl-3-hydroxybutyryl coenzyme a hydratase, an enzyme unique to isoleucine metabolism and enoyl coenzyme ...1978678016
pseudomonas ovalis ferredoxin: similarity to azotobacter and chromatium ferredoxins. 1978680138
chemical structure and biodegradability of halogenate aromatic compounds. substituent effects on 1,2-dioxygenation of benzoic acid.dioxygenation of substituted benzoic acids by whole cells of 3-chlorobenzoate-utilizing pseudomonas sp. b 13, benzoate-induced cells of alcaligenes eutrophus b 9 and toluate-grown cells of pseudomonas putida mt-2 was examined. electron-attracting substituents like halogen decreased the reaction rates of benzoate 1,2-dioxygenation. dioxygenation of substituted benzoic acids by p. putida mt-2 was mostly undisturbed by steric effects of the substituents. good correlation resulted between the log vr ...1978687664
amine dehydrogenase of pseudomonas putida: properties of the heme-prosthetic group.there was approximately five times more hemoprotein (amine dehydrogenase) in crude extracts obtained from pseudomonas putida grown on benzylamine than present in extracts from succinate-grown cells. the difference (reduced minus oxidized) spectrum of the purified enzyme possessed alpha,beta, and gamma bands at 550, 523, and 416 nm, respectively. the difference spectrum of the pyridine hemochrome derivative had absorption maxima at 416, 520, and 550 nm. these results, together with the fact that ...1978690082
a study of bacteria contaminating refrigerated cooked chicken; their spoilage potential and possible origin.cooked chicken was allowed to spoil in a normal kitchen refrigerator (variable temperature) and at a standard 4c. after 10 days' storage, bacteria were isolated from the chicken. it was found that the numbers of organisms at variable refrigeration temperature were tenfold higher than those at a uniform 4c. in an attempt to find the sources of contamination, swabs were made of different areas of the kitchen. many of the bacteria isolated from the spoiled chicken, were also isolated from the kitch ...1978701783
circular dichroism and magnetic circular dichroism of iron-sulfur proteins.circular dichroism (cd) and magnetic circular dichroism (mcd) spectra are reported for the 2-fe ferredoxins from pseudomonas putida and spirulina maxima, chromatium hipip, the 4-fe ferredoxin from bacillus stearothermophilus, and the 8-fe ferredoxin from clostridium pasteurianum. the spectral range spans the near-infrared, visible, and near ultraviolet. in all cases except oxidized 2-fe ferredoxins, electronic absorption is observed continuously from less than 5000 cm-1 to above 30,000 cm-1. the ...1978728385
chemical and spectral properties of putidamonooxin, the iron-containing and acid-labile-sulfur-containing monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida.gel chromatography indicates that putidamonooxin has a molecular weight of about 126,000. on the other hand, the amino acid composition and the iron-to-protein ratio point to a minimal molecular weight of 33,000 and 31,000 respectively. on sodium dodecylsulfate/polyacrylamide gel electrophoresis the enzyme migrated as a homogeneous band corresponding to a molecular weight of about 40,000. the number of spots found in the tryptic peptide map of the carboxymethylated and digested enzyme indicates ...1978729590
subunit and amino acid composition of l-arginine deiminase of pseudomonas putida. 1978729806
incorporation of [18o]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from pseudomonas putida.in the hydroxylation of the methyl group of p-cresol by an enzyme from pseudomonas putida the oxygen atom is derived from water. although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate.1978736904
p-cresol and 3,5-xylenol methylhydroxylases in pseudomonas putida n.c.i.b. 9896.pseudomonas putida n.c.i.b. 9869, when grown on 3,5-xylenol, hydroxylates the methyl groups on 3,5-xylenol and on p-cresol by two different enzymes. 3,5-xylenol methylhydroxylase, studied only in relatively crude extracts, requires nadh, is not active with p-cresol and is inhibited by cyanide, but not by co. the p-cresol methylhydroxylase requires an electron acceptor and will act under anaerobic conditions. it was purified and is a flavocytochrome c of mol.wt. approx. 114,000 consisting of two ...1978743215
the aromatic alcohol dehydrogenases in pseudomonas putida n.c.i.b. 9869 grown on 3,5-xylenol and p-cresol.whole cells of pseudomonas putida n.c.i.b 9869, when grown on either 3,5-xylenol or p-cresol, oxidized both m- and p-hydroxybenzyl alcohols. two distinct nad+-dependent m-hydroxybenzyl alcohol dehydrogenases were purified from cells grown on 3,5-xylenol. each is active with a range of aromatic alcohols, including both m- and p-hydroxybenzyl alcohol, but differ in their relative rates with the various substrates. an nad+-dependent alcohol dehydrogenase was also partially purified from p-cresol gr ...1978743216
dissociation of the nic plasmid aggregate in pseudomonas putida.the nic plasmid on conjugal transfer from pseudomonas convexa pc 1 to pseudomonas putida ppg 1 dissociates into an independent fertility factor t and a nontransmissible nic structural gene plasmid.1979762028
properties of anthranilate synthetase component ii from pseudomonas putida.the interaction of pseudomanas putida anthranilate synthetase component ii (as ii) with glutamine, glutamine analogs, and iodoacetamide has been investigated in order to clarify the initial steps in the mechanism for glutamine utilization. as ii is alkylated and irreversibly inactivated by covalent attachment of approximately 1 eg of l-2-amino-4-oxo-5-chloropentanoic acid (chloroketone) or 1 eq of iodoacetamide. alkylation of as ii by chloroketone involves initial formation of an enzyme-inhibito ...1976765343
fertility factors in pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors.the octane plasmid (oct) in pseudomonas putida strains has been shown to be transferred at low frequency. however, bacteria which had newly received this plasmid showed a transient increase in donor ability. using octane+ p. putida as the donor, the transfer of most chromosomal markers was shown to be independent of oct transfer, whereas the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on oct plasmid transfer. the presence of a fertility factor termed fpo h ...1979762014
direct hydroxylation in the biosynthesis of hydroxy fatty acid in lipid a of pseudomonas ovalis.it was found that pseudomonas ovalis iam 1177 had an abundance of hydroxy fatty acids such as 3-hydroxy-decanoic acid, 3-hydroxy-dodecanoic acid and 2-hydroxy-dodecanoic acid in the lipophilic part of the lipopolysaccharide fraction, which comprise 80% of total fatty acids. by using 18o2, it was shown that one oxygen atom from molecular oxygen was incorporated into 2-hydroxy-dodecanoic acid, but not into 3-hydroxy-decanoic acid. the incorporated oxygen atom was specifically located at the hydrox ...1979760794
isolation of plasmid deoxyribonucleic acid from pseudomonas putida.conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of pseudomonas putida containing degradative plasmids (cam, sal, oct, etc.) have been defined. these degradative plasmids could not be isolated by the usual procedure, whereas rp1, an r factor of the p group, present in the isogenic strain of p. putida, was isolated equally well by either the usual procedure or the modified procedure. characterization by electron microscopy of rp1 deoxy ...1976816778
superoxide dismutase from mycobacterium tuberculosis.1. a superoxide dismutase [ec 1.15.1.1] was purified about 275-fold with a yield of 34% from mycobacterium tuberculosis, strain h37ra (attenuated strain), grown on a sauton medium for two months. the purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis, and by analytical ultracentrifugation and sedimentation equilibrium studies. 2. the molecular weight of the enzyme was estimated to be approximately 88,000 by sedimentation equilibrium analysis. since the molecular weig ...1976828161
evidence for a transmissible catabolic plasmid in pseudomonas putida encoding the degradation of p-cresol via the protocatechuate ortho cleavage pathway. 1978751853
multiple forms of rat liver cytochrome p-450. immunochemical evidence with antibody against cytochrome p-448.purified hepatic cytochrome p-448 from 3-methylcholanthrene-treated rats was used to produce antibody in rabbits. the cytochrome p-448 antibody (igg fraction) isolated from immune rabbit serum is quite specific and precipitates purified rat liver cytochrome p-448 at low antibody to protein ratios when assayed by the ouchterlony double diffusion technique. purified hepatic cytochrome p-450 from phenobarbital-treated rats cross-reacts poorly with the cytochrome p-448 antibody as do purified rabbit ...1976815258
physiological function of the pseudomonas putida ppg6 (pseudomonas oleovorans) alkane hydroxylase: monoterminal oxidation of alkanes and fatty acids.pseudomonas putida ppg6 is able to utilize purified n-alkanes of six to ten carbon atoms for growth. it can also grow on the primary terminal oxidation products of these alkanes and on 1-dodecanol but not on the corresponding 2-ketones or 1,6-hexanediol, adipic acid, or pimelic acid. revertible point mutants can be isolated which have simultaneously lost the ability to grow on all five n-alkane growth substrates but which can still grow on octanol or nonanol. an acetate-negative mutant defective ...1975804473
magnitude of the equilibrium isotope effect on carbon-tritium bond synthesis.the exchange of tritium from 3hoh into the methyl group of pyruvate catalyzed by 6-phospho-2-keto-3-deoxygluconate aldolase (6-phospho-2-keto-3-deoxy-d-gluconate d-glyceraldehyde-3-phosphate-lyase, ec 4.1.2.14) of pseudomonas putida shows an equilibrium isotope effect of 0.78. from this value and the deuterium effect on the fumarase equilibrium (thomson, j.f. (1960) arch. biochem. biophys. 90, 1), one can calculate by use of the relative fractionation factors of hartshorn and shiner (hartshorn, ...1977836851
isolation of a mutant tol plasmid with increased activity and transmissibility from pseudomonas putida (arvilla) mt-2.strains with greater ability to dissimilate m-toluate were obtained from the wild-type pseudomonas putida (arvilla) mt-2 that harbors the tol plasmid. increased growth of a mutant strain on aromatic substrates was coupled with simultaneous increase in the activity of metapyrocatechase, an enzyme coded by the tol plasmid, without changing its catalytic properties. in the mutant and the wild-type strains, the inducer specificity and the induction kinetics of metapyrocatechase synthesis were the sa ...1977830645
p-cymene pathway in pseudomonas putida: initial reactions.initial reactions of the p-cymene pathway induced in pseudomonas putida pl have been reinvestigated. oxidation of the methyl group attached to the nucleus occurs in three steps to give p-cumic acid. the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate) and not as previously postulated via 3-hydroxy-p-cumate. there are three pieces of evidence for the physiological rol ...1977845117
p-cymene pathway in pseudomonas putida: ring cleavage of 2,3-dihydroxy-p-cumate and subsequent reactions.it was confirmed that 2,3-dihydroxy-p-cumate is a substrate for ring cleavage in pseudomonas putida pl-w after growth with p-cymene or p-cumate. this compound was oxidized to pyruvate, acetaldehyde, isobutyrate, and carbon dioxide by extracts of cells, and these products appear in equimolar amounts. the transient appearance of compounds and 2,3-dihydroxy-p-cumate to a yellow intermediate (lambda max, 345 nm) without decarboxylation. extracts of the benzene nucleus; this is followed by decarboxyl ...1977845118
properties of l-methionine gamma-lyase from pseudomonas ovalis.the distribution of bacterial l-methionine gamma-lyase (l-methionine methanethiollyase (deaminating) (ec 4.4.1.11) was investigated, and pseudomonas ovalis (ifo 3738) was found to have the highest activity of enzyme, which was inducibly formed by addition of l-methionine to the medium. l-methionine gamma-lyase, purified to homogeneity from ps. ovalis, has a molecular weight of about 173 000 and consists of nonidentical subunits (mol wt: 40 000 and 48 000). the enzyme exhibits absorption maxima a ...1977831771
modified sutter's arginine dihydrolase medium for pseudomonas speciation.sutter's arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. bromothymol blue is the indicator for alkalinity in sutter's medium. by adding glucose and lowering the ph of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. the modified sutter's medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic ...1977838673
purification and properties of homoprotocatechuate 2,3-dioxygenase from bacillus stearothermophilus.the enzyme 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing) (homoprotocatechuate 2,3-dioxygenase) was purified from the thermophilic organism bacillus stearothermophilus, grown with j-hydroxyphenylacetic acid as a source of carbon. the enzyme appeared to be homogeneous as judged by disc-gel electrophoresis and sedimentation equilibrium measurements. the average molecular weight determined by three independent procedures was 106,000; the protein was globular and was dissociated ...1977838683
isolation of metabolic plasmid dna from pseudomonas putida. 1977849300
mandelate racemase from pseudomonas putida. absence of detectable intermolecular proton transfer accompanying racemization.an equimolar mixture of dl-[alpha-2h]- and dl-[alpha-13c]mandelate, when incubated with mandelate racemase (ec 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. similarly, an equimolar mixture of unlabeled and dl-[alpha-2h,alpha-13c]mandelate, when incubated with the enzyme, shows conversion of doubly labeled mandelate to singly labeled mandelate, ...1977849410
molecular sizes and relationships of tol plasmids in pseudomonas.plasmid deoxyribonucleic acid was isolated from thirteen pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (tol plasmids). most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 x 10(6) to 202 x 10(6) daltons. some plasmids could be transformed into a pseudomonas putida strain to yield tol+ progeny. plasmids from 5 of the 13 st ...1977863855
myo-inositol transport system in pseudomonas putida.the kinetic features of the myo-inositol transport system in pseudomonas putida are reported. the system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. line-weaver-burk plots indicate the presence of two different systems, whose kt are 5 micrometer and 0.43 mm and whose v max are 7.9 and 27 nml/mg per min, respectively. transport activity of glucose-grown cells is very low. myo-inositol-grown cells lose the high-affinity sys ...1977893343
crystalline cis-benzene glycol dehydrogenase from pseudomonas putida. 1977859182
characterization of a spontaneously occurring mutant of the tol20 plasmid in pseudomonas putida mt20: possible regulatory implications.pseudomonas putida mt20 carries a plasmid (tol20) that codes for the enzymes responsible for the catabolism of toluene, m- and p-xylene to benzoate, and m- and p-toluate, respectively, followed by meta cleavage of the aromatic ring. growth on 5 mm benzoate selects very strongly for (i) strains that have been cured of the plasmid and (ii) strains with an intermediate growth pattern (the b3 phenotype) that retain the ability to grow on toluene, m-xylene, and benzoate but are unable to grow on m-to ...1977863853
distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...1977863854
failure of complex supplementation of minimal cultures to elicit a shift-up response in pseudomonas putida.the addition of complex supplements (particularly amino acids) to cultures of pseudomonas putida growing on a good carbon source did not result in a substantial increase in the growth rate. amino acids entered the cells within 30 s of addition and reached significant internal pool concentrations. endogenous amino acid biosynthesis was quickly inhibited (about 75%), with a substantial sparing of the original carbon source. within 20 min of supplementation significant respiration of added amino ac ...1976932677
molecular characterization of hydrocarbon degradative plasmids in pseudomonas putida. 1977901483
purification and partial amino acid sequence of the cyanogen bromide fragments of muconolactone isomerase from pseudomonas putida.muconolactone isomerase is shown to be resistant to proteolytic cleavage by trypsin. cyanogen bromide cleavage at the methionine residues of the polypeptide is at least 95% complete. six cyanogen bromide fragments are separated on deae-cellulose. one fragment is shown by amino acid analysis and carboxyl-terminal analysis to be an incomplete cleavage product. the five remaining fragments represent the entire polypeptide and have been ordered with respect to the entire muconolactone isomerase sequ ...1977901811
two modes of loss of the tol function from pseudomonas putida mt-2.some of a set of independently arising tol- (non toluate-utilising) derivatives of pseudomonas putida mt-2 have lost the unique plasmid present in the parent strain. in others this plasmid has suffered a deletion of a specific region of about 27 md.1977895716
metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in pseudomonas putida.two strains of pseudomonas putida isolated by enrichment cultures with orcinol as the sole source of carbon were both found to grow with resorcinol. data are presented which show that one strain (orc) catabolizes resorcinol by a metabolic pathway, genetically and mechanistically distinct from the orcinol pathway, via hydroxyquinol and ortho oxygenative cleavage to give maleylacetate, but that the other strain (o1) yields mutants that utilize resorcinol. one mutant strain, designated o1oc, was sh ...1976942589
the p-cymene pathway in pseudomonas putida pl: isolation of a dihydrodiol accumulated by a mutant. 1976942434
catechol oxygenases of pseudomonas putida mutant strains.investigation of a mutant strain of pseudomonas putida ncib 10015, strain psu-e1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. additional mutants of both wild-type and mutant strains psu-e1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. these findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate ...1976956121
involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by pseudomonas convexa.a microorganism capable of degrading dl-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as pseudomonas convexa. it was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. all the enzymes of the pathway were demonstrated in cell-free extracts. l-mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicot ...1976956122
purification and properties of methioninase from pseudomonas ovalis. 1976955094
crystallization and preliminary crystal data of iron-containing superoxide dismutase from pseudomonas ovalis.large single crystals of iron-containing superoxide dismutase from pseudomonas ovalis were prepared from 50% saturated ammonium sulfate solution, at ph 4.5, on gentle evaporation of the solvent at 4 degrees. the crystals were monoclinic, space group p2, with unit cell dimensions a = 81.9 a, b = 49.0 a, c = 61.0 a, and beta = 106 degrees. considerations of cell volume and protein molecular weight indicated 1 molecule of superoxide dismutase in the assymmetric unit, the smallest number reported s ...1976947910
[pseudomonas putida plasmid controlling the initial stages of naphthalene oxidation]. 1976949929
[microbial breakdown of caffeine (author's transl)].a bacterium, capable of growing aerobically with caffeine as its sole source of carbon and nitrogen, was isolated from soil and identified as pseudomonas putida sp. the breakdown of caffeine begins with stepwise demethylation, which leads via various n-methyl-purines to xanthine each step yielding formaldehyde. xanthine is then broken down via uric acid, allantoin, allantoic acid and further intermediates to urea and glyoxylic acid, which serves as the actual source of carbon.1976998047
enzyme evolution in a microbial community growing on the herbicide dalapon.a seven-membered microbial community capable of utilising the herbicide dalapon has been isolated by continuous-flow enrichment culture. the composition of this community has remained remarkably stable over thousands of hours in a dalapon-limited chemostat. during this period, however, one member of the community, pseudomonas putida, acquired the ability to grow on dalapon through the evolution of an extant dehalogenase.1976972691
current status of the sequence studies of the pseudomonas putida camphor hydroxylase system. 1976961531
aromatic-alcohol dehydrogenases from pseudomonas putida n.c.i.b. 9869. 19761001711
purification and properties of l-4-hydroxymandelate oxidase from pseudomonas convexa.an inducible membrane-bound l-4-hydroxymandelate oxidase (decarboxylating) from pseudomonas convexa has been solubilized and partially purified. it catalyzes the conversion of l-4-hydroxymandelic acid to 4-hydroxybenzaldehyde in a single step with the stoichiometric consumption of o2 and liberation of co2. the enzyme is optimally active at ph 6.6 and at 55 degrees c. it requires fad and mn2+ for its activity. the membrane-bound enzyme is more stable than the solubilized and purified enzyme. afte ...1976976259
combined chromosomal and plasmid encoded control for the degradation of phenol in pseudomonas putida. 19761001897
the uptake of 2-ketogluconate by pseudomonas putida.the uptake of 2-ketogluconate is inducible in pseudomonas putida: 2-ketogluconate, glucose, gluconate, glycerol and glycerate were each good nutritional inducers of this ability. 2-ketogluconate uptake obeyed saturation kinetics (apparent km in 2-ketogluconate-grown cells was 0.4 mm). 2-ketogluconate was transported against a concentration gradient, apparently in an unchanged state, and the process required metabolic energy, all of which indicate an active transport system. a number of independe ...19761015939
characterization of a benzoate permease mutant of pseudomonas putida.a spontaneous mutant of pseudomonas putida (prs 2017) has been isolated which is incapable of growth on benzoate, does not induce the enzymes of the catechol branch of the beta-ketoadipate pathway when grown in the presence of benzoate, cannot accumulate radioactively labeled benzoate, yet grows well with mandelate as sole source of carbon and energy. this strain apparently lacks a benzoate permease, which in the wild type shows a km of about 0.1 mm for benzoate, is inducible, and is not under t ...19761015938
creatine amidinohydrolase of pseudomonas putida: crystallization and some properties. 19761015832
the effect of a non-metabolizable analog on mandelate catabolism in pseudomonas putida.dl-2,3,4,5,6-pentafluoromandelic acid (pfm) specifically inhibits the growth of pseudomonas putida (atcc 12633) on medium containing mandelate as sole carbon and energy source by competitive inhibition of mandelate dehydrogenase. pfm is not metabolized and is neither an inducer of the mandelate catabolic enzymes nor an antagonist of induction. mutants resistant to the inhibitory effects of pfm (pfmr) were isolated; most prove to be superinducible, i.e. synthesize corrdinately the mandelate-speci ...19761015936
characterization of the growth of pseudomonas putida lp on lipoate and its analogues: transport, oxidation, sulphur source, and enzyme induction.pseudomonas putida lp, which grows on lipoate, nh4no3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-coa. d-, l-, or dl-lipoate serve equally well as carbon and sulphur sources. there was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortened analogues, but these, as well as lipoate, could supply the sulphur needed for growth when acetate was ...19751089758
lipoate metabolism in pseudomonas putida lp. 19751099990
transformation of pseudomonas putida and escherichia coli with plasmid-linked drug-resistance factor dna.conditions optimal for the transformation of pseudomonas putida and e. coli with a drug-resistance factor (rp 1) dna, which specifies resistance to carbenicillin, tetracycline, kanamycin, and neomycin, are described. the transformants retain all the fertility, incompatibility, and drug-resistance characteristics present in the parent. covalently-closed circular molecules of almost identical contour lengths have been isolated from the parent and the transformants. the frequency of transformation ...19751103151
improved method of selection for mutants of pseudomonas putida.optimum conditions for enrichment of mutants of pseudomonas putida in liquid culture were established using a procedure which combines n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis with an improved d-cycloserine selection.19751108792
regulation of the meta-cleavage of 4-hydroxyphenylacetic acid by pseudomonas putida. 19761027447
autogenous regulation of the inducible tryptophan synthase of pseudomonas putida.mutants blocked before indole-3-glycerol phosphate formation in the tryptophan biosynthetic pathway of p. putida ("early-blocked" mutants) are unable to use indole as a source of tryptophan for growth on minimal medium. the uninduced level of tryptophan synthase [ec 4.2.1.20; l-serine hydro-lyase (adding indole)] in such mutants was thought to be responsible for this property. we have shown that levels of indole higher than those previously tested will support growth of these mutants. in additio ...19751055401
purification and characterization of bacteriophage gh-i-induced deoxyribonucleic acid-dependent ribonucleic acid polymerase from pseudomonas putida.infection of pseudomonas putida by the bacteriophage gh-l-induced the synthesis of a novel dna-dependent rna polymerase. this gh-l-induced rna polymerase was purified to near homogeneity. it was shown to be distinct from the host rna polymerase (alpha-2 beta beta sigma) physically and in respect to many of its catalytic properties. the gh-l-induced rna polymerase was composed of a single polypeptide of approximately 98,000 molecular weight. the divalent metal ion requirement for in vitro rna syn ...19751112826
metabolism of resorcinylic compounds by bacteria. purification and properties of orcinol hydroxylase from pseudomonas putida 01.orcinol hydroxylase (ec 1.14.13.6), which catalyzes the first reaction of orcinol catabolism in pseudomonas putida 01, has been purified to homogeneity, and crystallized. orcinol hydroxylase catalyzes the hydroxylation of orcinol with equimolar consumption of o2 and nadh (or nadph) to 2, 3, 5-trihydroxytoluene, which is nonenzymically oxidized to a quinone. the visible absorption spectrum of the enzyme shows maxima at 373 and 454 nm and a shoulder at 480 nm. fad can be dissociated from the prote ...19751126936
the uptake of fructose by pseudomonas putida.fructose transport was not apparently affected in a number of pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturation kinetics (apparent km equal 0.3 mm). the fructose uptake system serves to transport glucose in addition to fructose. the entry of fructose into p.putida cells appears to be media ...19751115560
[generalized transduction of pseudomonas putida with a thermosensitive mutant of phage pf16h2]. 19761032694
pathways for the degradation of m-cresol and p-cresol by pseudomonas putida.a comparison of the oxidation rates of various compounds by whole cells of pseudomonas putida 3, 5 indicated that m-cresol is metabolized by oxidation to 3-hydroxybenzoate followed by hydroxylation to gentisate, the ring-fission substrate, when grown with 3, 5-xylenol. however, when m-cresol was the growth substrate, similar experiments suggested a different pathway involving a methyl-substituted catechol, and ring-fission by meta cleavage. assays of ring-fission enzymes in cell-free extracts co ...19751123316
immunochemical and compositional comparison of cytochrome p-450 cam of pseudomonas putida and p-450 lm of phenobarbital-induced rabbit liver microsomes.although highly purified cytochrome p-450 of pseudomonas putida (p-450 cam), and that from phenobarbital-induced rabbit liver microsomes (p-450 lm), differ markedly in their catalytic and physical properties, they show immunological cross reaction by competitive binding and inhibition of catalytic activity, and are of similar amino acid composition. upon treatment with cyanogen bromide they yield small heme-containing peptides of highly similar amino acid composition.19751155253
regulation of alkane oxidation in pseudomonas putida.we have studied the appearance of whole-cell oxidizing activity for n-alkanes and their oxidation products in strains of pseudomonas putida carrying the oct plasmid. our results indicate that the oct plasmid codes for inducible alkane-hydroxylating and primary alcohol-dehydrogenating activities and that the chromosome codes for constitutive oxidizing activities for primary alcohols, aliphatic aldehydes, and fatty acids. mutant isolation confirms the presence of an alcohol dehydrogenase locus on ...19751150626
induction of alkane hydroxylase proteins by unoxidized alkane in pseudomonas putida.in vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity.19751150630
the uptake of glucose and gluconate by pseudomonas putida.the uptake of glucose and gluconate is under inductive control in pseudomonas putida. glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. glucose and gluconate uptake obeyed saturation kinetics: the apparent km for glucose was 6 mm and that for gluconate was 0.5 mm. therefore, transport of both substrates appears to be mediated by enzyme-like carriers. glucose and gluconate are parallel inhibitors for their uptake9 strains selected for their ...19751134500
pseudomonas putida cytochrome p-450. the effect of complexes of the ferric hemeprotein on the relaxation of solvent water protons.with pulsed nuclear magnetic resonance techniques, the effects of various complexes of ferric cytochrome p-450 on the relaxation rate of bulk solution water protons have been determined. for the camphor, metyrapone, and 4-phenylimidazole complexes, the experimental results are consistent with outer sphere relaxation effects. however, for the substrate-free enzyme, the magnitude and temperature dependence of the paramagnetic relaxation effects indicate the presence of exchangeable protons in the ...19751158868
the stereochemistry at carbon 3 of pyruvate lyase condensation products. 2-keto-3-deoxygluconate-6-phosphate aldolase.in a condensation between [3-3h3]pyruvate and d-glyceraldehyde-3-p as catalyzed by 2-keto-3-deoxygluconate-6-p aldolase (ec 4.1.2.14) of pseudomonas putida, c--c synthesis occurred appreciably faster than c--3h bond breaking. since tritium is present in tritiated pyruvate in tracer amounts, this result showed hydrogen isotope discrimination in pyruvate deprotonation and suggests enolpyruvate generation to be at least partially rate-limiting in the condensation reaction. consequently, in a conden ...19751158885
mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450.anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ...19751182094
mössbauer investigations of high-spin ferrous heme proteins. ii. chloroperoxidase, horseradish peroxidase, and hemoglobin.reduced samples of chloroperoxidase, horseradish peroxidase, and deoxyhemoglobin were studied by mössbauer spectroscopy in strong magnetic fields. the intricate paramagnetic spectra of chloroperoxidase were evaluated in detail in the framework of a spin hamiltonian pertinent to high-spin ferrous iron. the studies strongly suggest that, in their reduced states, chloroperoxidase from caldariomyces fumago and cytochrome p-450 from pseudomonas putida have similar, if not identical ligand structures ...19751182095
new mechanisms for the biosynthesis and metabolism of 2-keto-l-gulonic acid in bacteria.l-sorbose is oxidized to 2-keto-l-gulonic acid (kga) via the following sequence of reactions which we call the "sorbosone pathway": l-sorbose in equilibrium l-sorbosone leads to kga. the first step is reversible and is mediated by enzymes found in a soluble fraction obtained from pseudomonas putida atcc 21812. although no cofactor requirements were found for the forward reaction, the reverse reaction clearly required nadh. enzymes for this nadh-dependent synthesis of l-sorbose could be different ...19751182275
metabolism of n-methylpurines by a pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen.pseudomonas putida, strain 40, originally isolated by enrichment on caffeine as the sole source of carbon and nitrogen, has been developed to grow on 0.5% caffeine. the organism will grow on any n-methyl derivative of xanthine containing one or more methyl groups at the 1, 3, or 7 positions. an investigation of the activities of resting cell suspensions and cell-free preparations together with the detection of metabolic intermediates suggest that caffeine is first metabolized by the action of an ...19751158847
[arthrobacter siderocapsulatus isolated from lake water].two microbial strains have been isolated from lake water. the strains oxidize ferrous compounds and manganese. by the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus siderocapsa. however, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus arthrobacter (corynebacteriaceae). since these microorganisms differ, by their cultural and morpho-physiological properties, f ...19751177780
physiological consequences of starvation in pseudomonas putida: degradation of intracellular protein and loss of activity of the inducible enzymes of l-arginine catabolism.we investigated the degradation of radioisotopically labeled intracellular protein in starved, intact cells of pseudomonas putida p2 (atcc 25571) and the regulation of this process. intracellular protein isotopically labeled with l-[4,5-3h]leucine during log-phase growth at 30 c is degraded at rates of 1 to 2%/h in log-phase cells and 7 to 9%/h in starved cells. rifampin, chloramphenicol, and tosyllysine chloromethylketone lower the rate of protein degradation by starved cells. addition to starv ...19751194237
alternative routes of aromatic catabolism in pseudomonas acidovorans and pseudomonas putida: gallic acid as a substrate and inhibitor of dioxygenases.when 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) was added to pseudomonase acidovorans growing at the expense of succinate, enzymes required for degrading homoprotocatechuate to pyruvate and succinate semialdehyde were strongly induced. these enzymes were effectively absent from cell extracts of the organism grown with 4-hydroxyphenylacetic acid, and this substrate was metabolized by the catabolic enzymes of the homogentisate pathway. two separate ring-fission dioxygenases for 3,4,5 ...19751194238
metabolism of toluene and xylenes by pseudomonas (putida (arvilla) mt-2: evidence for a new function of the tol plasmid.pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m-xylene, and p-xylene on a transmissible plasmid, tol. these compounds are degraded by oxidation of one of the methyl substituents via the corresponding alcohols and aldehydes to benzoate and m- and p-toluates, respectively, which are then further metabolised by the meta pathway, also coded for by the tol plasmid. the specificities of the benzyl alcohol dehydrogenase and the benzaldehyde dehydrogenase for their three ...19751176436
an inducible amidase from pseudomonas striata. 19751191288
exchange reactions catalyzed by methioninase from pseudomonas putida.highly purified methioninase from pseudomonas putida, which catalyzes alpha, gamma-elimination reactions of homocysteine and its s-substituted derivatives as well as alpha, beta-elimination reactions of cysteine and its derivatives, was found to catalyze exchange reactions between the substituent at the gamma-carbon of homocysteine substrates and exogenously added alkanethiols, forming the corresponding s-alkylhomocysteines. it also catalyzed similar beta-exchange reactions between cysteine and ...19751213994
a kinetic and structural characterization of adenosine-5'-triphosphate: ribonucleic acid adenylyltransferase from pseudomonas putida.a catalytic and structural study of atp:rna adenylyltransferase (ec 2.7.7.19) from the particulate fraction of pseudomonas putida was made. during the large-scale purification of this enzyme, designated adenylyltransferase b, a previously undetected atp-incorporating activity, designated adenylyltransferase a, was observed. adenylyltransferases a and b were indistinguishable catalytically; however, they differed in their chromatographic and sedimentation properties. adenylyltransferases a and b ...19751191706
[r factor found in pseudomonas putida]. 19751240230
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