Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| 3-chloro-d-alanine chloride-lyase (deaminating) of pseudomonas putida cr 1-1. | 1984 | 6427787 | |
| [effect of plasmids from various incompatibility groups on the development of bacteriophages specific to pseudomonas aeruginosa and pseudomonas putida]. | the aim of this work was to study the effect of plasmids belonging to different incompatibility groups on the growth of bacteriophages in pseudomonas aeruginosa and pseudomonas putida strains. the growth of bacteriophages was shown to be limited most often due to the presence in cells of plasmids belonging to the p-2 incompatibility group. plasmids of the inc p-2 group differed from one another in the spectrum of bacteriophages whose growth they limited. phages whose growth was suppressed in str ... | 1984 | 6431239 |
| inactivation of the pseudomonas striata broad specificity amino acid racemase by d and l isomers of beta-substituted alanines: kinetics, stoichiometry, active site peptide, and mechanistic studies. | mechanism-based inactivators were used to probe the active site of the broad specificity amino acid racemase from pseudomonas striata. kinetic parameters for the inactivation of the racemase with both stereoisomers of beta-fluoroalanine, beta-chloroalanine, and o-acetylserine were determined. by use of 14c-labeled o-acetylserines, the stoichiometry of inactivator binding was found to be one inactivator bound per enzyme subunit. the plp-dependent enzyme contains one coenzyme per subunit, and afte ... | 1984 | 6439237 |
| [phosphate and glucose accumulation by pseudomonas cultures in relation to their arsenic resistance]. | the effect of arsenite and arsenate on 14c-glucose and 32-p-phosphate transport was studied in the cells of pseudomonas aeruginosa 561 sensitive to arsenite and in the cells of pseudomonas putida 18 oxidizing arsenite and resistant to arsenic. transport and accumulation of phosphate and glucose were inhibited in the presence of arsenite in the cells of p. aeruginosa 561 whereas arsenate inhibited only phosphate accumulation. arsenite and arsenate had hardly any effect at the initial transport ra ... | 1984 | 6439982 |
| [effect of humic acids from the brown coal of the aleksandriyskoya field on pseudomonas putida growth]. | 1980 | 6446021 | |
| effect of restricted aeration on catabolism of cholic acid by two pseudomonas species. | examination of some previously isolated bile acid-utilizing pseudomonas strains showed that pseudomonas sp. atcc 31752, together with other fluorescent strains, can be assigned to pseudomonas putida biotype b, whereas pseudomonas sp. atcc 31753, like most other nonfluorescent strains, is an unrecognized phenotype. a study was made of the growth of these two species at 25 degrees c and ph 7.0 in a fermentor with 2.5 g of sodium cholate liter-1 as sole carbon source, and the catabolism of the chol ... | 1984 | 6476826 |
| structural analysis of the cysteine-containing peptides from the major 3-methylcholanthrene-induced isozyme of cytochrome p-450 (p-450c) in rat liver microsomes. | cytochrome p-450c, the major 3-methylcholanthrene-inducible isozyme of cytochrome p-450 in rat liver microsomes, was subjected to proteolytic digestion after s-carboxymethylation of the protein, and the peptides were resolved by high-pressure liquid chromatography. since it is now recognized that cytochromes p-450 contain a thiolate as the axial fifth ligand of the heme, seven peptides containing eight cysteines were subjected to microsequence analysis. one cysteine-containing peptide (tsa-56) w ... | 1984 | 6477879 |
| aromatic acids are chemoattractants for pseudomonas putida. | a quantitative capillary assay was used to show that aromatic acids, compounds that are chemorepellents for escherichia coli and salmonella sp., are chemoattractants for pseudomonas putida prs2000. the most effective attractants were benzoate; p-hydroxybenzoate; the methylbenzoates; m-, p-, and o-toluate; salicylate; dl-mandelate; beta-phenylpyruvate; and benzoylformate. the chemotactic responses to these compounds were inducible. taxis to benzoate and m-toluate was induced by beta-ketoadipate, ... | 1984 | 6501217 |
| tol plasmid can prevent induction of chemotactic responses to aromatic acids. | growth conditions that elicited positive chemotaxis to benzoate and m-toluate in tol- pseudomonas putida cells failed to elicit taxis to these compounds in tol+ cells. the inability of tol+ cells to respond to these aromatic acids appears to be due to the preferential expression of tol-encoded genes for aromatic degradation over chromosomally encoded genes. expression of chromosomal genes for aromatic degradation is required for cells to form beta-ketoadipate, the inducer of benzoate and m-tolua ... | 1984 | 6501222 |
| isolation and characterization of pseudomonas putida ppf1 mutants defective in the toluene dioxygenase enzyme system. | pseudomonas putida ppf1 degraded toluene via a dihydrodiol pathway to tricarboxylic acid cycle intermediates. the initial reaction was catalyzed by a multicomponent enzyme, toluene dioxygenase, which oxidized toluene to (+)-cis-1(s),2(r)-dihydroxy-3-methylcyclohexa-3,5-diene (cis-toluene dihydrodiol). the enzyme consisted of three protein components: nadh-ferredoxintol oxidoreductase (reductasetol), ferredoxintol, and a terminal oxygenase which is an iron-sulfur protein (isptol). mutants blocked ... | 1984 | 6501223 |
| novel reactivity of cytochrome p-450-cam. methyl hydroxylation of 5,5-difluorocamphor. | the interaction of the camphor hydroxylating p-450 isolated from pseudomonas putida grown on camphor (p-450-cam) with 5,5-difluorocamphor, a substrate analog in which the two methylene hydrogens at the normal site of hydroxylation have been replaced with fluorine, has been examined. this compound binds tightly to the enzyme with a dissociation constant and uv-visible absorption spectrum identical to that observed with d-camphor. in the presence of the reconstituted p-450-cam system, 5,5-difluoro ... | 1984 | 6501299 |
| metabolism of alpha-terpineol by pseudomonas incognita. | details of the metabolism of alpha-terpineol by pseudomonas incognita are presented. degradation of alpha-terpineol by this organism resulted in the formation of a number of acidic and neutral metabolites. among the acidic metabolites, beta-isopropyl pimelic acid, 1-hydroxy-4-isopropenyl-cyclohexane-1-carboxylic acid, 8-hydroxycumic acid, oleuropeic acid, cumic acid, and p-isopropenyl benzoic acid have been identified. neutral metabolites identified were limonene, p-cymene-8-ol, 2-hydroxycineole ... | 1984 | 6525582 |
| formaldehyde dehydrogenase from pseudomonas putida: a zinc metalloenzyme. | the nad+-dependent formaldehyde dehydrogenase from pseudomonas putida c-83 was found to contain 4 gram atoms of zinc per mol, corresponding to 2 gram atoms of zinc per subunit monomer. treatment of the enzyme with o-phenanthroline resulted in removal of 1 gram atom of zinc per subunit and caused a complete inactivation of the enzyme. the activity lost was restored by the addition of zinc ions, by which the zinc content was also reversed to almost the same level as that of the native enzyme. anot ... | 1984 | 6526822 |
| chemical modification of tyrosine residues at the active centre of cytochrome p-450 cam. | soluble cytochrome p-450 cam from pseudomonas putida (ec 1.14.14.1) was chemically modified with tetranitromethane. at least five out of totally nine tyrosine residues are accessible to nitration as shown by tryptic peptide mapping using hplc. modification in the presence of the inhibitor metyrapone and subsequent peptide mapping indicate the location of one tyrosine residue at the active centre of cytochrome p-450 cam. | 1984 | 6532454 |
| phenolic 9,10-secosteroids as products of the catabolism of bile acids by a pseudomonas sp. | the obligate aerobe, pseudomonas putida atcc 31752, efficiently utilises bile acids as a source of carbon and energy for growth and maintenance. when aeration is considerably restricted, a consequence to the catabolism of the bile acids in a fermentor is an accumulation of certain steroidal catabolites. evidence is presented to show that among these are hydroxy-9,10-seco-1,3,5 (10)-androstratriene-9, 17-diones and those from four of the common bile acids, cholic, chenodeoxycholic, hyodeoxycholic ... | 1984 | 6537051 |
| oxidation of glycine by pseudomonas putida requires a specific lipoamide dehydrogenase. | pseudomonas putida produces two lipoamide dehydrogenases with molecular weights of 49,000 and 56,000 designated lpd-val and lpd-glc, respectively. lpd-val is required for oxidation of valine, since it is specifically utilized as the e3 component of branched-chain keto acid dehydrogenase. since glycine oxidation by bacteria and mammals also requires lipoamide dehydrogenase, we desired to determine which lipoamide dehydrogenase would be used by the p. putida glycine oxidation system. when grown in ... | 1984 | 6546487 |
| structure of iron superoxide dismutase from pseudomonas ovalis at 2.9-a resolution. | the three-dimensional structure of the iron-containing superoxide dismutase (ec 1.15.1.1) from pseudomonas ovalis has been determined at 2.9-a resolution by the method of multiple isomorphous replacement. the molecule is a dimer of two identical subunits with the iron atom per monomer. the conformation of the enzyme is completely different from that of the eukaryotic copper-zinc superoxide dismutase. each subunit consists of about 50% alpha-helix plus three strands of antiparallel pleated sheet. ... | 1983 | 6575382 |
| coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome p-450d cdna from rat liver. | we determined the coding nucleotide sequence of the mrna for a 3-methylcholanthrene-inducible cytochrome p-450, p-450d, of rat liver by sequence analysis of cloned cdnas. the predicted amino acid sequence of the cytochrome is composed of 513 amino acids, and its nh2-terminal sequence of 30 amino acids completely coincides with that reported from analysis of the purified cytochrome p-450d. the amino acid composition of the deduced sequence also agrees well with that determined from the purified p ... | 1984 | 6584898 |
| camphor hydroxylase of pseudomonas putida: vestiges of sequence homology in cytochrome p-450cam, putidaredoxin, and related proteins. | the amino acid sequences of cytochrome p-450cam and putidaredoxin of the camphor hydroxylase [camphor, reduced-putida-ferredoxin:oxygen oxidoreductase (5-hydroxylating), ec 1.14.15.1] of pseudomonas putida are compared to each other and then to the sequences of bovine adrenodoxin and cytochrome b5. the comparisons reveal areas of homology indicating that these four proteins may share a common evolutionary origin. moreover, homologous segments can be recognized by proper alignment of the sequence ... | 1984 | 6584899 |
| p-chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl coa: beta-ketoadipate coa transferase. | beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) and succinyl coa: beta-ketoadipate transferase (ec 2.8.3.6) catalyze consecutive metabolic reactions in bacteria. the enzymes appear to be members of different families of related proteins. enzymes within the enol-lactone hydrolase family appear to have diverged so extensively that common ancestry sometimes is not directly evident from comparison of nh2-terminal amino acid sequences of the proteins. amino acid sequences at or near the active ... | 1984 | 6591865 |
| reversal by dna amplifications of an unusual mutation blocking alkane and alcohol utilization in pseudomonas putida. | we analyzed the reversion of strains carrying alk208, a mutation in the alkbac (alkane utilization) region of the pseudomonas cam-oct plasmid. reversion of alk208 was stimulated 25 to 75-fold by small doses of uv-irradiation. all alkane hydroxylase-positive (alkb+) revertants proved to be aliphatic alcohol dehydrogenase-positive (alkc+) as well, whereas alkc+ revertants could be either alkb+ or alkb-. most of the alkb- alkc+ partial revertants produced alkc- segregants at measurable frequencies. ... | 1984 | 6597334 |
| [oral infection wtih pseudomonas putida]. | 1984 | 6599728 | |
| bacterial interference with urine osmolality measurements. | we report bacterial interference with urine osmolality measurements using an instrument based on the principle of freezing point depression. although the exact nature of the interfering activity has not been defined, the phenomenon is associated with a bacterium, identified as pseudomonas putida, and is removed from the specimens by filtration at 0.45 micron. the bacteria led to osmometer dysfunction presumably by acting as a nidus for crystallization and preventing proper supercooling of specim ... | 1983 | 6616812 |
| [improved procedure for isolation and purification of methionine gamma-lyase from pseudomonas putida]. | an improved, simplified and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme--methionine gamma-lyase from pseudomonas putida. the method includes five steps instead of seven steps in previous procedure with a good yield of the enzyme. the purified enzyme was shown to be homogeneous by the criteria of disc gel electrophoresis. the highly homogeneous preparations of the enzyme exhibited the absorption maxima at 280 and 420 nm. the detailed studies on ... | 1983 | 6623989 |
| properties and roles of bacterial symbionts of polyvinyl alcohol-utilizing mixed cultures. | from several polyvinyl alcohol (pva)-utilizing mixed cultures, two component bacterial strains essential for pva utilization were isolated, and their properties and roles in pva utilization were studied. each pair of essential component strains consisted of a type i strain, which produced a pva-degrading enzyme and constituted the predominant population of the mixed culture in pva, and a type ii strain, which produced a certain growth stimulant for the former strain. all of the type i strains we ... | 1983 | 6639015 |
| heme binding and substrate-protected cysteine residues in p-450cam. | reactions of the pseudomonas putida cytochrome p-450-substrate complex or enzyme alone with 14c-labeled iodoacetic acid have been investigated at ph 7.0. after subsequent conversion of all of the cysteine residues to s-beta-carboxymethylcysteinyl residues, tryptic peptides of the derivative were separated by either high performance liquid chromatography or two dimensional electrophoresis, and their amino acid compositions and partial sequences were determined. all but cysteine residue-134 reacte ... | 1983 | 6639664 |
| the microbial oxygenation of the benzylisoquinoline alkaloid laudanosine. | the microbial transformation of the benzylisoquinoline alkaloid laudanosine by a strain of pseudomonas putida gives a metabolite in which o-demethylation of 1 methoxyl group of ring c, and introduction of 1 ketonic oxygen at c9 and 1 phenolic oxygen at ring c have occurred. also, o-methylcoripalline is formed in this transformation. | 1983 | 6641902 |
| ligand binding to heme proteins. an evaluation of distal effects. | the o2, co, and alkyl isocyanide-binding properties of a variety of vertebrate and invertebrate heme proteins have been compared in detail to those of protoheme mono-3-(1-imidazoyl)-propylamide monomethyl ester in aqueous suspensions of soap micelles. the proteins examined include: cytochrome p-450cam from pseudomonas putida, beef heart cytochrome c oxidase, yeast cytochrome c peroxidase, alpha and beta subunits of human hemoglobin, sheep hemoglobin, carp hemoglobin, sperm whale myoglobin, horse ... | 1983 | 6643477 |
| rapid reaction studies on the oxygenation reactions of catechol dioxygenase. | the reaction of oxygen with catechol 1,2-dioxygenase from pseudomonas arvilla atcc 23974 in complex with catechol, 4-methylcatechol, and 4-fluorocatechol has been studied using single turnover stopped flow spectrophotometry. two sequential enzyme intermediates have been resolved and their visible spectra characterized by computer-assisted methods. these intermediates are spectrally similar to those observed in a similar study with protocatechuate dioxygenase (bull, c., ballou, d. p., and otsuka, ... | 1983 | 6643492 |
| enzymes of the beta-ketoadipate pathway in pseudomonas putida: kinetic and magnetic resonance studies of the cis,cis-muconate cycloisomerase catalyzed reaction. | steady-state kinetic analysis of the divalent metal ion requiring cis,cis-muconate cycloisomerase catalyzed interconversion of cis,cis-muconate and (+)-muconolactone obeys michaelis-menten kinetics and the haldane relationship from ph 6.2 to 8.3. the ph vs. kcat/km profiles suggest free-enzyme apparent pka values of 6.2 and 7.4: the reciprocal behavior of the data with respect to the latter pka value is consistent with base-acid catalysis by the enzyme involving proton removal from the lactone a ... | 1983 | 6652062 |
| enzymes of the beta-ketoadipate pathway in pseudomonas putida: primary and secondary kinetic and equilibrium deuterium isotope effects upon the interconversion of (+)-muconolactone to cis,cis-muconate catalyzed by cis,cis-muconate cycloisomerase. | primary and secondary kinetic and secondary equilibrium deuterium isotope effect studies on the cis,cis-muconate cycloisomerase catalyzed interconversion of cis,-cis-muconate (ccm) and (+)-muconolactone (ml) have been performed. the primary and solvent kinetic deuterium isotope effects upon vmax for the reactions of (+)-[5r-2h]muconolactone in water (hoh) and (+)-muconolactone in deuterium oxide (dod) to form cis,cis-muconate are about 2.5-2.6 with the heavier isotopic reactions being the slower ... | 1983 | 6652063 |
| [degradation of 3-chlorobenzoic acid by a pseudomonas putida strain]. | a pseudomonas putida strain 87 capable of assimilating 3-chlorobenzoic acid as a sole source of carbon and energy (3cba+) was isolated. treatment with mitomycin c eliminated the 3cba+ phenotype in 1% of cells in the population. the 3cba+ phenotype was transferred at a low frequency in the process of conjugation to other bacteria belonging to the genus pseudomonas. determinants localized on the plasmid are presumed to be responsible for the capability to assimilate 3-chlorobenzoic acid. a scheme ... | 1983 | 6664313 |
| properties and function of malate enzyme from pseudomonas putida. | malate enzyme (l-malate: nadp+ oxidoreductase (oxalacetate-decarboxylating, ec 1.1.1.40)) has been purified from pseudomonas putida to 99 per cent homogeneity by heat, ammonium suphate fractionation, gel filtration and anion exchange chromatography. sodium dodecylsulphate-(sds)-polyacrylamide disc gel electrophoresis analysis showed an approximate tetrameric subunit with a molecular weight of 52,000. the purified enzyme showed a ph optimum between 8.0 and 8.5 (for tris-hcl buffer) and required b ... | 1983 | 6673742 |
| synthesis of s-(carboxymethyl)-d-cysteine by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1. | s-(carboxymethyl)-d-cysteine, which is an important component of semisynthetic cephalosporin, mt-141, was enzymatically synthesized. s-(ethoxy-carbonyl-methyl)-d-cystein was synthesized from 3-chloro- d-alanine and ethyl thioglycolate by the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1 and subsequently hydrolyzed by alkali. the synthesized s-(carboxymethyl)-d-cysteine was isolated from a large scale reaction mixture and identified physicochemicall ... | 1983 | 6679711 |
| hydroxyproline 2-epimerase of pseudomonas. subunit structure and active site studies. | hydroxyproline 2-epimerase of pseudomonas putida was purified to homogeneity by an improved procedure. the native enzyme consists of two probably identical subunits. alkylation of the active site with labeled reagents resulted in the loss of 80-85% of the activity but the incorporation of only one alkyl group even though the active site contains a cys residue from each of the two subunits. this result suggests that the enzyme shows half-site reactivity. the labeled enzyme was further subjected t ... | 1984 | 6706934 |
| plasmid-determined silver resistance in pseudomonas stutzeri isolated from a silver mine. | a silver-resistant strain of pseudomonas stutzeri was isolated from a silver mine. it harbored three plasmids, the largest of which (pkk1; molecular weight, 49.4 x 10(6)) specified silver resistance. plasmid pkk1 was apparently nonconjugative but could be transferred to pseudomonas putida by mobilization with plasmid r68.45. | 1984 | 6715284 |
| purification of bacterial l-methionine gamma-lyase. | a rapid procedure for the purification of l-methionine gamma-lyase from pseudomonas putida icr 3460 by deae- toyopearl 650m and deae-sephadex a-50 column chromatography is presented. the enzyme was purified with an average yield of 75% and showed about 10-fold higher specific activity than the enzyme from p. putida (= p. ovalis) ifo 3738 reported previously (h. tanaka, n. esaki , and k. soda (1976) febs lett. 66, 307-311). the present enzyme has a molecular weight of about 172,000 and consists o ... | 1984 | 6742420 |
| proton magnetic resonance studies of 7fe ferredoxins. three redox states of the [4fe-4s] cluster in a pseudomonas ovalis ferredoxin. | the oxidizability of a redox couple, [4fe-4s], in a 7fe ferredoxin extracted from pseudomonas ovalis was monitored by 1h-nmr. the iron-sulfur cluster in the ferredoxin was not only reducible (nagayama et al., 1983) but also oxidizable in its native form. this result provided the first verification of 3 redox states for a redox center in ferredoxin, 4fe, in the native form of the protein. | 1984 | 6745423 |
| degradation of 3-chlorobenzoate in soil by pseudomonads carrying biodegradative plasmids. | degradation of continuously added 3-chlorobenzoate (3-cb) was studied in samples of chernozem soil. soil columns were inoculated with pseudomonas putida growing on 3-cb and carrying the biodegradation plasmid and with pseudomonas aeruginosa incapable of growth on 3-cb and carrying the inserted biodegradation plasmid pbs 2 determining ortho-cleavage of the aromatic ring. while the 3-cb degradation was observed in both inoculated variants, the native microflora of the soil under study was incapabl ... | 1984 | 6745818 |
| amino acid sequence of the pseudomonas putida cytochrome p-450. i. sequences of tryptic and clostripain peptides. | in order to elucidate the complete amino acid sequence of pseudomonas putida cytochrome p-450, tryptic digestion was performed on the s-carboxymethylated enzyme. although cleavage did not occur at every lysyl and arginyl bond, 31 tryptic peptides ranging in size from 1 to 55 residues were isolated. these were sequenced by manual edman degradation and carboxypeptidase digestion. overlaps of some od these tryptic peptides were obtained by data obtained from partial edman degradation and amino acid ... | 1982 | 6752138 |
| [preparation of protoplast-like structures of pseudomonas putida and their reversion to bacterial forms]. | a technique is proposed for the preparation of pseudomonas putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing mono-and divalent cations. pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 m) for 90 min at the ph 7.9 to 8.0 is a prerequisite for the formation of 'protoplasts'. under these conditions, the yield of 'protoplasts' reached 99.9% of the total cell number. the protoplast-like structures are capable of reversin ... | 1982 | 6755196 |
| insights into the active site of the cytochrome p-450 haemoprotein family--a unifying concept based on structural considerations. | 1. cytochromes p-450cam of pseudomonas putida and p-450lm-2 of rabbit liver, and to a lesser extent also adrenocortical mitochondrial cytochromes p-450(11) beta and p-450scc, were investigated by active site-targeted reagents and by immunochemical techniques. the results of these studies and of the alignment of the amino acid sequences of cytochromes p-450cam and of phenobarbital-induced rat-liver p-450lm support the following conclusions. 2. cytochrome p-450 haemoproteins follow a common archit ... | 1982 | 6762774 |
| mutants defective in isomerase and decarboxylase activities of the 4-hydroxyphenylacetic acid meta-cleavage pathway in pseudomonas putida. | degradation of 2-hydroxy-5-carboxymethylmuconic semialdehyde, the ring fission product of the 4-hydroxyphenylacetate meta-cleavage pathway, by mutant strains p23x19 and p23x16 of pseudomonas putida nci b 9865 was studied. both mutants were unable to grow on either 4-hydroxyphenylacetate of 3,4-dihydroxyphenylacetate. cell extracts of p23x19, grown in the presence of 3,4-dihydroxyphenylacetate, degraded the ring fission product to a compound that accumulated and had maximum uv absorption at 300 n ... | 1980 | 6769900 |
| reactivation studies on putidamonooxin -- the monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida. | 1980 | 6772176 | |
| headspace analysis of volatile metabolites of pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry. | gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of pseudomonas aeruginosa and 1 strain each of pseudomonas cepacia, pseudomonas putida, pseudomonas putrefaciens, pseudomonas fluorescens, and pseudomonas maltophilia. all strains of pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. the other strains failed to produce 2-aminoacetophenone. ... | 1980 | 6775012 |
| expression of e. coli genes carried by hybrids of plasmid rp4. | hybrids of the rp4 plasmid, containing bacteriophage mu and chromosomal genes of escherichia coli, were transferred into salmonella typhimurium, pseudomonas putida, pseudomonas aeruginosa and proteus mirabilis. the individual genes of the arginine, histidine, leucine and threonine operons were expressed in these microorganisms. | 1980 | 6777258 |
| inhibition, but not uncoupling, of respiratory energy coupling of three bacterial species by nitrite. | the effect of nitrite on respiratory energy coupling of three bacteria was studied in light of a recent report that nitrite acted as an uncoupling agent with paracoccus denitrificans grown under denitrifying conditions. our determinations of proton translocation stoichiometry of pseudomonas putida (aerobically grown), pseudomonas aeruginosa, and p. denitrificans (grown both aerobically and under denitrifying conditions) showed nitrite inhibition of proton-to-oxidant stoichiometry, but not uncoup ... | 1980 | 6777373 |
| comparative immunochemical studies of cytochrome p-450cam of pseudomonas putida and of cytochrome p-450scc of bovine adrenocortical mitochondria. | 1980 | 6780340 | |
| evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-coa transferases from acinetobacter calcoaceticus and pseudomonas putida. | homogeneous beta-ketoadipate succinyl-coa transferase (ec 2.8.3.6) preparations were obtained from extracts of acinetobacter calcoaceticus and pseudomonas putida. gel filtration indicated that the respective transferases have similar molecular weights of 108,000 and 109,000; each transferase appears to have an alpha 2 beta 2 oligomeric structure formed by association of nonidentical subunits with a molecular weight of about 25,000. the subunits were separated by sodium dodecyl sulfate-gel electr ... | 1981 | 6780551 |
| evolutionary relationships among gamma-carboxymuconolactone decarboxylases. | gamma-carboxymuconolactone decarboxylase (ec 4.1.1.44) from azotobacter vinelandii resembled the isofunctional enzymes from acinetobacter calcoaceticus and pseudomonas putida. all three decarboxylases appeared to be hexamers formed by association of identical subunits of about 13,300 daltons. the a. vinelandii and p. putida decarboxylases cross-reacted immunologically with each other, and the nh2-terminal amino acid sequences of the enzymes differed in no more than 7 of the first 36 residues. in ... | 1981 | 6783616 |
| [isolation and analysis of phage-resistant pseudomonas putida mutants using new bacteriophages]. | three new bacteriophages of pseudomonas putida ppg1 have been isolated. bacteriophages differ in many characteristics and are unrelated. phage-resistant mutants of pseudomonas putida ppg1 are selected using these phages and pf16 bacteriophage. no lysogenic variants were detected among these mutants. they have been distributed in seven groups according to their resistance to bacteriophages. the resistance to pmw phage was caused by at least two different mutations. some of the phage-resistant mut ... | 1981 | 6785151 |
| synthesis of d-cysteine from 3-chloro-d-alanine and hydrogen sulfide by 3-chloro-d-alanine hydrogen chloride-lyase (deaminating) of pseudomonas putida. | 1981 | 6791643 | |
| transfer of nitrogen fixation genes into pseudomonas putida isolated from finnish tundra soil. | the plasmid prd1 containing the nif genes from klebsiella pneumoniae was transferred by conjugation from escherichia coli to pseudomonas putida isolated from the tundra soil. 6-cyanopurine, acetylene reduction and immunological tests showed that the nif genes were not expressed in p. putida. existence of the nif genes in p. putida transconjugants was detected by transferring them to e. coli c600, which does not fix nitrogen. existence of the nif genes in e. coli c600 transconjugants was detected ... | 1981 | 6798929 |
| [integration of the genome of the mu-like pseudomonas aeruginosa bacteriophage d3112 into plasmid rp4 and its hybrid plasmid transfer into pseudomonas putida and escherichia coli c600 bacteria]. | the genome of a mu-like bacteriophage d3112 specific for pseudomonas aeruginosa was integrated in vivo into the rp4 plasmid. the fact of integration has been proved by two experiments: 1. the loss of rp4 plasmid is accompanied by loss of d3112 prophage; 2. transfer of the plasmid by conjugation from pseudomonas aeruginosa into bacteria of other species - p. putida pgg1 or escherichia coli c600 leads to the occurrence of clones of these species which liberate phage capable of growing on the lawn ... | 1982 | 6799358 |
| delta1-piperideine-2-carboxylate reductase of pseudomonas putida. | pseudomonas putida metabolizes d-lysine to delta 1-piperideine-2-carboxylate and l-pipecolate. the second step of this catabolic pathway is catalyzed by delta 1-piperideine-2-carboxylate reductase. this enzyme was isolated and purified from cells grown on dl-lysine as substrate. the enzyme was very unstable, resulting in low recovery of activity and low purity after a six-step purification procedure. the enzyme had a ph optimum of 8.0 to 8.3. the km values for delta 1-piperideine-2-carboxylate a ... | 1982 | 6801013 |
| isolation of alginate-producing mutants of pseudomonas fluorescens, pseudomonas putida and pseudomonas mendocina. | spontaneous alginate-producing (muc) variants were isolated from strains of pseudomonas fluorescens, p. putida and p. mendocina at a frequency of 1 in 10(8) by selecting for carbenicillin resistance. the infrared spectrum of the bacterial exopolysaccharide was typical of an acetylated alginate similar to that previously described in azotobacter vinelandii and in mucoid variants of p. aeruginosa. mucoid variants were not isolated from p. stutzeri, p. pseudoalcaligenes, p. testosteroni, p. diminut ... | 1981 | 6801192 |
| use of buffered hypochlorite solution for disinfecting fibrescopes. | the possible use of sodium hypochlorite solution buffered to ph 7.6 and containing 100 ppm available chlorine (avcl) for disinfecting fibrescopes was investigated. a flexible fibrescope experimentally contaminated with pseudomonas putida, mycobacterium fortuitum, or bacillus subtilis spores was effectively disinfected within 10 m in repeatedly and without any observable adverse effect on the instrument. the corrosive nature of buffered hypochlorite was investigated by immersing various fibrescop ... | 1982 | 6802880 |
| d-malic enzyme of pseudomonas fluorescens. | by the enrichment culture technique 14 gram-negative bacteria and two yeast strains were isolated that used d(+)-malic acid as sole carbon source. the bacteria were identified as pseudomonas putida, pseudomonas fluorescens, pseudomonas aeruginosa and klebsiella aerogenes. in cell-free extracts of p. fluorescens and p. putida the presence of malate dehydrogenase, d-malic enzyme (nad-dependent) and l-malic enzyme (nadp-dependent) was demonstrated. d-malic enzyme from p. fluorescens was purified. s ... | 1982 | 6804238 |
| monoclonal antibodies against pseudomonas aeruginosa outer membrane antigens: isolation and characterization. | hybridomas secreting monoclonal antibodies specific for pseudomonas aeruginosa outer membrane antigens were isolated. one of the antibodies was highly specific for the o antigen of the lipopolysaccharide of international antigen typing scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotypes. this monoclonal antibody was able to agglutinate heat-killed bacterial cells as well as lipopolysacc ... | 1982 | 6809625 |
| [expression of the genome of mu-like phage d3112 specific for pseudomonas aeruginosa in escherichia coli and pseudomonas putida cells]. | the behavior of escherichia coli cells carrying rp4 plasmid which contains the genome of a mu-like d3112 phage specific for pseudomonas aeruginosa was studied. two different types of d3112 genome expression were revealed in e. coli. the first is bp4-dependent expression. in this case, expression of certain d3112 genes designated as "kil" only takes place when rp4 is present. as a result, cell division stops at 30 degrees c and cells form filaments. cell division is not blocked at 42 degrees c. t ... | 1982 | 6811373 |
| 3-chloro-d-alanine chloride-lyase (deaminating) of pseudomonas putida cr 1.1. purification and characterization of a novel enzyme occurring in 3-chloro-d-alanine-resistant pseudomonads. | a novel enzyme catalyzing cleavage of 3-chloro-d-alanine to pyruvate, ammonia, and chloride ion is distributed in some pseudomonads which have a resistance to high concentrations of 3-chloro-d-alanine. pseudomonas putida cr 1-1 (aku 867) was found to have the highest activity of enzyme, which was inducibly formed by the addition of 3-chloro-d-alanine to the medium. the enzyme, tentatively called 3-chloro-d-alanine chloride-lyase, was purified from p.l putida cr 1-1 in seven steps. after the last ... | 1982 | 6815180 |
| complete nucleotide sequence of the metapyrocatechase gene on the toi plasmid of pseudomonas putida mt-2. | metapyrocatechase which catalyzes the oxygenative ring cleavage of catechol to form alpha-hydroxymuconic epsilon-semialdehyde is encoded by the xyle gene on the tol plasmid of pseudomonas putida mt-2. we have cloned the xyle region in escherichia coli and determined the nucleotide sequence of the dna fragment of 985 base pairs around the gene. the fragment included only one open translational frame of sufficient length to accommodate the enzyme. the predicted amino acid sequence consisted of 307 ... | 1983 | 6826546 |
| steroid 21-hydroxylase (cytochrome p-450) from porcine adrenocortical microsomes: microsequence analysis of cysteine-containing peptides. | the steroid 21-hydroxylase cytochrome p-450 from porcine adrenocortical microsomes was purified to homogeneity. the protein exhibited two nh2-terminal sequences, one of which was identical with the first but lacking the nh2-terminal methionine. the sequence was extremely hydrophobic but had little homology to the 17 alpha-hydroxylase/c17,20-lyase isolated from neonatal porcine testes or to rat or rabbit liver microsomal cytochromes p-450. the cysteine-containing fragments of the s-carboxymethyla ... | 1983 | 6830757 |
| synthesis of d-cysteine-related amino acids by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1. | using the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1, s-methyl-, s-ethyl-, s-n-propyl-, s-n-butyl-, s-phenyl-, s-benzyl-, s-(2,3-dihydroxypropyl)- and s-(2-hydroxyethyl)-d-cysteine were synthesized from 3-chloro-d-alanine and methyl-, ethyl-, n-propyl-, n-butyl-, phenyl-, benzyl-mercaptans, alpha-thioglycerol and 2-mercaptoethanol, respectively. the enzymatically synthesized d-cysteine-related amino acids were isolated from the large scale react ... | 1983 | 6838588 |
| camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by pseudomonas putida. | previously, pseudomonas putida was shown to degrade (+)-camphor, and cleavage of the first ring of the bicyclic structure involved two monooxygenases (a hydroxylase and a ring oxygen-inserting enzyme), a dehydrogenase, and spontaneous cleavage of an unstable oxygenation product (lactone). cleavage of the second ring was not demonstrated but was assumed also to occur by ring oxygen insertion, since the predicted oxygenation product was extracted from whole-cell incubation systems. our investigati ... | 1983 | 6848481 |
| [cytotoxic effect of methionine-gamma-lyase on neoplastic cells in culture]. | the influence of methionine-gamma-lyase from pseudomonas putida on dna synthesis by caov and l-8 cell lines has been studied. the agent has been demonstrated to inhibit the incorporation of 3h-thymidine into l-8 cell line and to have no effect on caov cells. | 1983 | 6850097 |
| immunological relatedness of histidine ammonia-lyases from some species of pseudomonas: taxonomic implication. | 1. histidine ammonia-lyases (histidase ec 4.3.1.3) from pseudomonas testosteroni ncib 10808 and pseudomonas putida ncib 10807 were purified and specific antibody was raised to each separately in a rabbit. 2. immunological cross-reactions of each antibody to histidine ammonia-lyases from various species of pseudomonas were examined by the enzyme inhibition test. 3. the immunological data obtained suggest that these pseudomonas species can be classified into three groups. these cross-reactions ten ... | 1983 | 6862094 |
| isolation of two lipoamide dehydrogenases from pseudomonas putida grown on valine. | 1981 | 6895028 | |
| control of meta-cleavage degradation of 4-hydroxyphenylacetate in pseudomonas putida. | synthesis of enzymes of the 4-hydroxyphenylacetate meta-cleavage pathway was studied in pseudomonas putida wild-type strain p23x1 (ncib 9865) and mutant strains which had either structural or regulatory gene mutations. induction studies with mutant strains each defective in an enzyme of the pathway showed that 4-hydroxyphenylacetate induced the hydroxylase and that 3,4-dihydroxyphenylacetate induced the 2,3-oxygenase, aldehyde dehydrogenase, isomerase, decarboxylase, and hydratase. this showed t ... | 1981 | 6895079 |
| isolation of a specific lipoamide dehydrogenase for a branched-chain keto acid dehydrogenase from pseudomonas putida. | we purified lipoamide dehydrogenase from cells of pseudomonas putida ppg2 grown on glucose (lpd-glu) and lipoamide dehydrogenase from cells grown on valine (lpd-val), which contained branched-chain keto acid dehydrogenase. lpd-glu had a molecular weight of 56,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and lpd-val had a molecular weight of 49,000. the ph optimum for lpd-glu for reduced nicotinamide adenine dinucleotide oxidation was 7.4, compared with ph 6.5 f ... | 1981 | 6895373 |
| [phage pf16 interrelationships with pseudomonas putida bacteria. i. unstable transductants and mutants of pseudomonas putida pfg1 resistant to phage pf16]. | the frequencies of transduction of the chromosomal genes by pf16 in pseudomonas putida pgg1 are dependent on the marker transduced and unpredictable. histidine and isoleucine-valine positive transductants, which are resistant to pf16, have been selected in the crosses with low (about 10(-8) for phage units) frequencies of transduction. some of these transductants carry new mutations. the transductants are unstable for phage resistance and histidine or isoleucine-valine positive characters. there ... | 1980 | 6928126 |
| construction of a partial diploid for the degradative pathway encoded by the tol plasmid (pwwo) from pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyir gene. | 1980 | 6929031 | |
| excision of the 40kb segment of the tol plasmid from pseudomonas putida mt-2 involves direct repeats. | 1981 | 6950198 | |
| molecular and functional analysis of the tol plasmid pwwo from pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. | the genetic organization of the pseudomonas putida plasmid pwwo-161, which encodes enzymes for the degradation of toluene and related aromatic hydrocarbons, has been investigated by transposition mutagenesis and gene cloning. catabolic genes were localized to two clusters, one for upper pathway (hydrocarbon leads to carboxylic acid) enzymes and the other for lower pathway (carboxylic acid leads to tricarboxylic acid cycle) enzymes, that are separated by a 14-kilobase dna segment. the physical or ... | 1981 | 6950388 |
| primary structure of serratia marcescens anthranilate synthase component ii. | the amino acid sequence of anthranilate synthase component ii (as ii) from serratia marcescens was determined. the cysteine residue essential for glutamine utilization was alkylated selectively by iodo [1-14c]acetamide prior to separation of the two protein components of anthranilate synthase. the isolated as ii then was subjected to cleavage by cyanogen bromide and by trypsin after citraconylation to obtain overlapping fragments. as ii is a single polypeptide chain of 192 residues having a calc ... | 1980 | 6986371 |
| regulation of enzymes of the 3,5-xylenol-degradative pathway in pseudomonas putida: evidence for a plasmid. | constitutive synthesis of enzymes responsible for methyl group oxidation in 3,5-xylenol degradation and an associated p-cresol methylhydroxylase in pseudomonas putida ncib 9869 was shown by their retention at high specific activities in cells transferred from 3,5-xylenol medium to glutamate medium. the specific activities of other enzymes of the 3,5-xylenol pathway declined upon removal of aromatic substrate, consistent with their inducible control. specific activities of the methyl-oxidizing en ... | 1980 | 6989805 |
| [nucleoside catabolism study of pseudomonas putida and pseudomonas trifolii]. | no activity of specific nucleoside phosphorylases was detected in pseudomonas putida; as the result, its cells cannot grow on various nucleosides using them as carbon sources. insignificant growth on ribonucleosides, viz. inosine and uridine, should be attributed to the activity of hydrolases in the cell. in contrast, pseudomonas trifolii is capable of normal growth on various nucleosides using them as a sole source of carbon. accordingly, the cell extracts display the activities of enzymes invo ... | 1980 | 6993879 |
| effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography. | high-resolution pyrolysis gas-liquid chromatography was applied to three bacteria (escherichia coli nctc 9001, pseudomonas putida (ncib 9494, and staphylococcus aureus nctc 8532) grown under a variety of conditions. changing the culture medium drastically altered the quantitative aspects of the pyrograms of all three organisms, but the effects of culture time and incubation temperature were less severe. mathematical analysis of the relative peak heights showed that four peaks could be used to di ... | 1980 | 6999989 |
| chemical structure and inhalation toxicity of lipopolysaccharides from bacteria on cotton. | lipopolysaccharides from different bacteria isolated from cotton were purified and chemically analyzed. their pulmonary toxicity to animals was tested in inhalation tests. lipopolysaccharides from agrobacterium and xanthomonas were shown to differ from the others in that they contained no heptose and no non-hydroxylated fatty acids with a chain length of 12, 14, or 16 carbon atoms. lipopolysaccharides from pseudomonas putida, enterobacter agglomerans, and klebsiella oxytoca were found to cause a ... | 1980 | 7000706 |
| inability of the api-20e system to speciate the fluorescent group of pseudomonads. | one thousand randomly selected clinical isolates of the fluorescent group of pseudomonas were speciated in parallel by the api-20e system and by a classic microbiologic 17-test battery. the classic battery identified the isolates as 993 pseudomonas aeruginosa, five pseudomonas putida, and two pseudomonas fluorescens. to augment the p. putida and p. fluorescens data, 52 reference isolates were also tested in parallel. api-20e was found to identify 99% of p. aeruginosa as p. aeruginosa. however, t ... | 1981 | 7008576 |
| molecular cloning of tol genes xylb and xyle in escherichia coli. | the xylb and xyle genes in the tol plasmid of pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pbr322 in escherichia coli for detailed mapping. the xylb gene was mapped in a 2.9-kilobase region within the bamhi bc fragment of ptn2, an in vivo rp4-tol recombinant, whereas the xyle gene was mapped in a 1.8-kilobase region within the bamhi bd fragment. the directions of transcription of these genes were deduced f ... | 1981 | 7009570 |
| incorporation of substrate cell lipid a components into the lipopolysaccharide of intraperiplasmically grown bdellovibrio bacteriovorus. | the composition of bdellovibrio bacteriovorus lipopolysaccharide (lps) was determined for cells grown axenically and intraperiplasmically on escherichia coli or pseudomonas putida. the lps of axenically grown bdellovibrios contained glucose and fucosamine as the only detectable neutral sugar and amino sugar, and nonadecenoic acid (19:1) as the predominant fatty acid. additional fatty acids, heptose, ketodeoxyoctoic acid, and phosphate were also detected. lps from bdellovibrios grown intraperipla ... | 1981 | 7024249 |
| tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: enzyme regulation and dna structure. | wr211 and wr216 are derivatives of halobenzoate-degrading pseudomonas sp. strain b13 into which the 117-kilobase tol degradative plasmid pww0 has been transferred from pseudomonas putida mt-2. wr211 has lost the ability to grow on the tol-specific substrate m-xylene but retains the ability to grow on its metabolite, m-toluate. an analysis of the induction of enzymes was consistent with wr211 carrying a nonfunctional regulatory gene, xy1r, wr216 is a spontaneous derivative of wr211 which grows on ... | 1982 | 7061391 |
| tol plasmid pww0 in constructed halobenzoate-degrading pseudomonas strains: prevention of meta pathway. | the hybrid pathway for chlorobenzoate metabolism was studied in wr211 and wr216, which were derived from pseudomonas sp. b13 by acquisition of tol plasmid pww0 from pseudomonas putida mt-2. chlorobenzoates are utilized readily by these strains when meta cleavage of chlorocatechols is suppressed. when wr211 utilizes 3-chlorobenzoate (3cb), the expression of catechol 2,3-dioxygenase (c23o) and the catabolic activities for chloroaromatics via the ortho pathway coexist as a consequence of inactivati ... | 1982 | 7061393 |
| [catalase activity of a pseudomonas putida strain oxidizing arsenic]. | trivalent arsenic ions added to the medium stimulated the catalase activity of a pseudomonas putida 18 culture correlating with the rate of arsenic oxidation. as soon as the process of arsenic oxidation stopped, the activity of catalase decreased. apparently, the production of h2o2, its decomposition by catalase and oxidation of as(iii) to as(v) by the formed oxygen are a device for detoxication of trivalent arsenic acquired by the cell in the course of evolution. therefore, oxidation of trivale ... | 1982 | 7070307 |
| preliminary crystallographic data on cytochrome p-450cam. | the cytochrome p-450cam monooxygenase from pseudomonas putida has been crystallized in three forms designated orthorhombic i, tetragonal i, and orthorhombic ii. conditions and parameters of crystal growth are presented along with the preliminary x-ray diffraction data including heavy atom derivatives. | 1982 | 7107613 |
| the reaction of 4-deoxy-4-fluoro-d-glucose with an outer membrane protein of pseudomonas putida. | 1982 | 7117530 | |
| comparison of kinetics of active tetracycline uptake and active tetracycline efflux in sensitive and plasmid rp4-containing pseudomonas putida. | membrane vesicles prepared from tetracycline-sensitive cells of pseudomonas putida took up tetracycline by an active transport system with an apparent km of 2.5 mm and a vmax of 50 nmol min-1 mg protein-1. in contrast, resistance determinant rp4-containing p. putida had an active high-affinity efflux system for tetracycline with a km of 2.0 to 3.54 microm and a vmax of 0.15 nmol min-1 mg protein-1. thus, the efflux system of tetracycline-resistant p. putida(rp4) had an average of 1,000-fold grea ... | 1982 | 7118827 |
| subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from pseudomonas arvilla c-1. | benzoate-1,2-dioxygenase system from pseudomonas arvilla c-1 consists of two protein components, benzoate-1,2-dioxygenase reductase and benzoate-1,2-dioxygenase (yamaguchi, m., and fujisawa, h. (1980) j. biol. chem. 255, 5058-5063). benzoate-1,2-dioxygenase exhibited two protein bands (alpha and beta) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their molecular weights were estimated to the 50,000 and 20,000, respectively. the intensities of protein staining on polyacrylamid ... | 1982 | 7130163 |
| amino acid sequence of the pseudomonas putida cytochrome p-450. ii. cyanogen bromide peptides, acid cleavage peptides, and the complete sequence. | 1982 | 7130171 | |
| stimulation of 3,4-dichloroaniline mineralization by aniline. | mineralization of free and of humus-bound 3,4-dichloroaniline (dca) by a pseudomonas putida strain isolated by analog enrichment was greatly enhanced in the presence of aniline. the addition of aniline to soil that contained 0.2 to 100 micrograms of dca per g in free or in humus-bound form increased the mineralization rates of dca severalfold. within the concentration ranges tested, absolute mineralization of dca per unit time was positively correlated with both increasing dca and increasing ani ... | 1982 | 7138005 |
| [plasmid pbs241 of pseudomonas putida controlling biphenyl degradation]. | 1982 | 7140506 | |
| active transport of benzoate in pseudomonas putida. | benzoate uptake in pseudomonas putida is mediated by an active transport system capable of accumulating benzoate against a 150-fold concentration gradient when subsequent metabolism is blocked by mutation. initial benzoate transport rates are inhibited by cccp, sodium azide, arsenate and dccd. uptake is stimulated by including a respirable carbon source during preincubation of the bacteria. the initial uptake rate and the atp pool levels are not correlated and no periplasmic components were foun ... | 1982 | 7142957 |
| stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of pseudomonas putida pp3: evidence for two different dehalogenation mechanisms. | pseudomonas putida pp3 grew on dl-2-monochloropropionate (2mcpa) with a release of chloride ions consistent with the dechlorination of both isomers. the organism grew on either d- or l-2mcpa. dehalogenase activity in cell-free extracts showed that both d- and l-2mcpa were dehalogenated. pseudomonas putida pp3 contains two dehalogenases, and studies with the separated enzymes revealed that the fraction i enzyme used both d- and l-2mcpa, the rate of dechlorination of l-2mcpa being 80% of the rate ... | 1982 | 7142958 |
| 4-hydroxy-4-methyl-2-ketoglutarate aldolase from pseudomonas putida. | 1982 | 7154956 | |
| the metabolism of cyclohexanecarboxylic acid and 3-cyclohexenecarboxylic acid by pseudomonas putida. | a strain of pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. a coa-mediated beta-oxidation pathway was induced for the metabolism of the compound. the organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in the presence of 3-cyclohexenecarboxylic acid were induced to metabolize cyclohexanecarboxylic acid, benzoic acid, and catechol. evidence is presented that 3-cyclohexenecarboxylic ac ... | 1982 | 7168830 |
| sensitivity of 341 non-fermentative gram-negative bacteria to seven beta-lactam antibiotics. | susceptibility of 341 isolates of non-fermentative gram-negative bacteria to carbenicillin, piperacillin, cefoperazone, moxalactam, cefotaxime, ceftizoxime, and n-formimidoyl thienamycin was determined by the agar dilution and disc diffusion methods. piperacillin was the most active agent against pseudomonas aeruginosa, thienamycin the most active against pseudomonas fluorescens and pseudomonas putida, and moxalactam the most active against pseudomonas maltophilia. piperacillin and thienamycin w ... | 1982 | 7173181 |
| [pseudomonas putida ppg1 resistance to different bacteriophages]. | cris-cross resistance analysis has been accomplished for some pseudomonas putida ppg1 mutants resistant to pf16, af, tf and pmw phages. on the basis of the results, a formal scheme of the receptor sites for these phages on the bacterial cell surface was drawn. study of the sensitivity of p. putida ppg1 phage-resistant mutants an unexpected phenomenon. some of the phages specific to p. putida b-21 and b-31 which did not adsorb on the ppg1 strain acquired the ability on certain types of the ppg1 p ... | 1981 | 7198071 |
| control of microorganisms in the rhizosphere of wheat by inoculation of seeds with pseudomonas putida and by foliar application of urea. | after inoculation of wheat seeds with various bacterial strains germination of plants was usually inhibited at first but growth was stimulated later. after inoculation with pseudomonas putida k 11 producing physiologically active compounds the total number of bacteria increased together with the bacteria: fungi ratio in the rhizosphere. these characteristic were further increased after foliar application of urea due to increased root exudation. dry mass of upper wheat parts was about 14--80% hig ... | 1981 | 7203287 |
| naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase. | 1,2-dihydroxynaphthalene oxygenase was purified from pseudomonas putida ncib 9816 grown on naphthalene as the sole source of carbon and energy. the enzyme had a subunit molecular weight of 19,000 and in a medium containing phosphate buffer, 1 mm mercaptoethanol, and 10% (vol/vol) ethanol had a native molecular weight greater than 275,000. the enzyme required fe2+ for activity. it was inactivated slowly on standing, and inactivation was accelerated by dilution with aerated buffers and by h2o2. ba ... | 1980 | 7204331 |