Publications

genetically engineered microorganisms for the detection of explosives' residues.	the manufacture and use of explosives throughout the past century has resulted in the extensive pollution of soils and groundwater, and the widespread interment of landmines imposes a major humanitarian risk and prevents civil development of large areas. as most current landmine detection technologies require actual presence at the surveyed areas, thus posing a significant risk to personnel, diverse research efforts are aimed at the development of remote detection solutions. one possible means p ...	2015	26579085
minion analysis and reference consortium: phase 1 data release and analysis.	the advent of a miniaturized dna sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. the minion™ access programme (map) was initiated by oxford nanopore technologies™ in april 2014, giving public access to their usb-attached miniature sequencing device. the minion analysis and reference consortium (marc) was formed by a subset of map participants, with the aim of evaluating and providing standard protocols and re ...	2015	26834992
c-di-gmp regulates motile to sessile transition by modulating msha pili biogenesis and near-surface motility behavior in vibrio cholerae.	in many bacteria, including vibrio cholerae, cyclic dimeric guanosine monophosphate (c-di-gmp) controls the motile to biofilm life style switch. yet, little is known about how this occurs. in this study, we report that changes in c-di-gmp concentration impact the biosynthesis of the msha pili, resulting in altered motility and biofilm phenotypes in v. cholerae. previously, we reported that cdgj encodes a c-di-gmp phosphodiesterase and a δcdgj mutant has reduced motility and enhanced biofilm form ...	2015	26505896
integrative and conjugative elements (ices): what they do and how they work.	horizontal gene transfer plays a major role in microbial evolution, allowing microbes to acquire new genes and phenotypes. integrative and conjugative elements (ices, a.k.a. conjugative transposons) are modular mobile genetic elements integrated into a host genome and are passively propagated during chromosomal replication and cell division. induction of ice gene expression leads to excision, production of the conserved conjugation machinery (a type iv secretion system), and the potential to tra ...	2015	26473380
biocide effects of volatile organic compounds produced by potential biocontrol rhizobacteria on sclerotinia sclerotiorum.	six rhizobacteria isolated from common bean and able to protect bean plants from the common bacterial blight (cbb) causal agent, were in vitro evaluated for their potential antifungal effects toward different plant pathogenic fungi, mostly soil-borne. by dual culture assays, the above bacteria resulted producing diffusible and volatile metabolites which inhibited the growth of the majority of the pathogens under study. in particular, the latter substances highly affected the mycelium growth of s ...	2015	26500617
biconnectivity of the cellular metabolism: a cross-species study and its implication for human diseases.	the maintenance of stability during perturbations is essential for living organisms, and cellular networks organize multiple pathways to enable elements to remain connected and communicate, even when some pathways are broken. here, we evaluated the biconnectivity of the metabolic networks of 506 species in terms of the clustering coefficients and the largest biconnected components (lbcs), wherein a biconnected component (bc) indicates a set of nodes in which every pair is connected by more than ...	2015	26490723
the dualistic nature of integrative and conjugative elements.	integrative and conjugative elements (ices) are mobile genetic elements that play a key role in bacterial adaptation. such elements are found in almost every bacterial genera and species, and often code for adaptive traits conferring selective advantages to their host. ices maintain by integrating into and replicating along with a replicon of the host genome. ices can propagate by conjugative transfer toward a recipient cell following excision from the replicon as a circular covalently-closed mo ...	2015	26942046
whole-genome sequence of pseudomonas putida strain uasws0946, a highly ammonia-tolerant nitrifying bacterium isolated from sewage sludge aerobic granules.	we report here the genome of pseudomonas putida strain uasws0946, a highly ammonia-tolerant nitrifying strain isolated from sewage sludge aerobic granules, which displays adequate genetic equipment for soil depollution, sludge treatment, and biological fertilization in agriculture.	2015	26450728
organophosphate hydrolase in conductometric biosensor for the detection of organophosphate pesticides.	the research has developed an enzyme biosensor for the detection organophosphate pesticide residues. the biosensor consists of a pair of screen-printed carbon electrode (spces). one of electrodes contains immobilized organophosphate hydrolase (oph) on a chitosan membrane by cross-linking it with glutaraldehyde. the area of the electrodes was optimized to 3, 5, and 7 mm(2). the oph was isolated from pseudomonas putida, and was purified by the ammonium sulfate precipitation method, with 6444 ppm ( ...	2015	26483607
pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response.	soil bacteria typically thrive in water-limited habitats that cause an inherent matric stress to the cognate cells. matric stress gives rise to accumulation of intracellular reactive oxygen species (ros), which in turn may induce oxidative stress, and even promote mutagenesis. however, little is known about the impact of ros induced by water limitation on bacteria performing important processes as pollutant biodegradation in the environment. we have rigorously examined the physiological conseque ...	2015	26445482
the industrial age of biocatalytic transamination.	during the last decade the use of ω-transaminases has been identified as a very powerful method for the preparation of optically pure amines from the corresponding ketones. their immense potential for the preparation of chiral amines, together with their ease of use in combination with existing biocatalytic methods, have made these biocatalysts a competitor to any chemical methodology for (asymmetric) amination. an increasing number of examples, especially from industry, shows that this biocatal ...	2015	26726292
deterioration to extinction of wastewater bacteria by non-thermal atmospheric pressure air plasma as assessed by 16s rdna-dgge fingerprinting.	the use of cold plasma jets for inactivation of a variety of microorganisms has recently been evaluated via culture-based methods. accordingly, elucidation of the role of cold plasma in decontamination would be inaccurate because most microbial populations within a system remain unexplored owing to the high amount of yet uncultured bacteria. the impact of cold atmospheric plasma on the bacterial community structure of wastewater from two different industries was investigated by metagenomic-based ...	2015	26500637
immune-relevant and antioxidant activities of vitellogenin and yolk proteins in fish.	vitellogenin (vtg), the major egg yolk precursor protein, is traditionally thought to provide protein- and lipid-rich nutrients for developing embryos and larvae. however, the roles of vtg as well as its derived yolk proteins lipovitellin (lv) and phosvitin (pv) extend beyond nutritional functions. accumulating data have demonstrated that vtg, lv and pv participate in host innate immune defense with multifaceted functions. they can all act as multivalent pattern recognition receptors capable of ...	2015	26506386
structure-odor relationships of linalool, linalyl acetate and their corresponding oxygenated derivatives.	linalool 1 is an odorant that is commonly perceived as having a pleasant odor, but is also known to elicit physiological effects such as inducing calmness and enhancing sleep. however, no comprehensive studies are at hand to show which structural features are responsible for these prominent effects. therefore, a total of six oxygenated derivatives were synthesized from both 1 and linalyl acetate 2, and were tested for their odor qualities and relative odor thresholds (ots) in air. linalool was f ...	2015	26501053
influence of substrates on the surface characteristics and membrane proteome of fibrobacter succinogenes s85.	although fibrobacter succinogenes s85 is one of the most proficient cellulose degrading bacteria among all mesophilic organisms in the rumen of herbivores, the molecular mechanism behind cellulose degradation by this bacterium is not fully elucidated. previous studies have indicated that cell surface proteins might play a role in adhesion to and subsequent degradation of cellulose in this bacterium. it has also been suggested that cellulose degradation machinery on the surface may be selectively ...	2015	26492413
draft genome sequence of the polychlorinated biphenyl-degrading bacterium pseudomonas stutzeri kf716 (nbrc 110668).	pseudomonas stutzeri kf716 (nbrc 110668) utilizes biphenyl as a sole source of carbon and energy and degrades polychlorinated biphenyls. here, we report the first draft genome sequence of a biphenyl-degrading strain of the species p. stutzeri.	2015	26494664
active site diversification of p450cam with indole generates catalysts for benzylic oxidation reactions.	cytochrome p450 monooxygenases are useful biocatalysts for c-h activation, and there is a need to expand the range of these enzymes beyond what is naturally available. a panel of 93 variants of active self-sufficient p450cam[tyr96phe]-rhfred fusion enzymes with a broad diversity in active site amino acids was developed by screening a large mutant library of 16,500 clones using a simple, highly sensitive colony-based colorimetric screen against indole. these mutants showed distinct fingerprints o ...	2015	26664590
assessing the groundwater quality at a saudi arabian agricultural site and the occurrence of opportunistic pathogens on irrigated food produce.	this study examines the groundwater quality in wells situated near agricultural fields in saudi arabia. fruits (e.g., tomato and green pepper) irrigated with groundwater were also assessed for the occurrence of opportunistic pathogens to determine if food safety was compromised by the groundwater. the amount of total nitrogen in most of the groundwater samples exceeded the 15 mg/l permissible limit for agricultural irrigation. fecal coliforms in densities > 12 mpn/100 ml were detected in three o ...	2015	26445052
performance evaluation of the verigene gram-positive and gram-negative blood culture test for direct identification of bacteria and their resistance determinants from positive blood cultures in hong kong.	a multicenter study was conducted to evaluate the diagnostic performance and the time to identifcation of the verigene blood culture test, the bc-gp and bc-gn assays, to identify both gram-positive and gram-negative bacteria and their drug resistance determinants directly from positive blood cultures collected in hong kong.	2015	26431434
dissemination of carbapenemases producing gram negative bacteria in the middle east.	the emergence and spread of carbapenemase-producing bacteria, that hydolyze most β-lactams, including carbapenems, are a major concern of public health system worldwide, particularly in the middle east area. since the plasmids harboring resistance genes could be spread across other bacterial populations, detection of carbapenemase-producing organisms has become more problematic. these organisms produce different types of enzymes including the most prevalent types including kpc, vim, imp, ndm, an ...	2015	26719779
mass spectrometric identification and toxicity assessment of degraded products of aflatoxin b1 and b2 by corymbia citriodora aqueous extracts.	this study explores the detoxification potential of corymbia citriodora plant extracts against aflatoxin b1 and b2 (afb1; 100 μg l(-1) and afb2; 50 μg l(-1)) in in vitro and in vivo assays. detoxification was qualitatively and quantitatively analyzed by tlc and hplc, respectively. the study was carried out by using different parameters of optimal temperature, ph and incubation time period. results indicated that c. citriodora leaf extract(s) more effectively degrade afb1 and afb2 i.e. 95.21% and ...	2015	26423838
light-induced structural changes in a short light, oxygen, voltage (lov) protein revealed by molecular dynamics simulations-implications for the understanding of lov photoactivation.	the modularity of light, oxygen, voltage (lov) blue-light photoreceptors has recently been exploited for the design of lov-based optogenetic tools, which allow the light-dependent control of biological functions. for the understanding of lov sensory function and hence the optimal design of lov-based optogentic tools it is essential to gain an in depth atomic-level understanding of the underlying photoactivation and intramolecular signal-relay mechanisms. to address this question we performed mol ...	2015	26484348
functional characterization and stability improvement of a 'thermophilic-like' ene-reductase from rhodococcus opacus 1cp.	ene-reductases (ers) are widely applied for the asymmetric synthesis of relevant industrial chemicals. a novel er oyero2 was found within a set of 14 putative old yellow enzymes (oyes) obtained by genome mining of the actinobacterium rhodococcus opacus 1cp. multiple sequence alignment suggested that the enzyme belongs to the group of 'thermophilic-like' oyes. oyero2 was produced in escherichia coli and biochemically characterized. the enzyme is strongly nadph dependent and uses non-covalently bo ...	2015	26483784
variability in subpopulation formation propagates into biocatalytic variability of engineered pseudomonas putida strains.	pivotal challenges in industrial biotechnology are the identification and overcoming of cell-to-cell heterogeneity in microbial processes. while the development of subpopulations of isogenic cells in bioprocesses is well described (intra-population variability), a possible variability between genetically identical cultures growing under macroscopically identical conditions (clonal variability) is not. a high such clonal variability has been found for the recombinant expression of the styrene mon ...	2015	26483771
plant derived substances with anti-cancer activity: from folklore to practice.	plants have had an essential role in the folklore of ancient cultures. in addition to the use as food and spices, plants have also been utilized as medicines for over 5000 years. it is estimated that 70-95% of the population in developing countries continues to use traditional medicines even today. a new trend, that involved the isolation of plant active compounds begun during the early nineteenth century. this trend led to the discovery of different active compounds that are derived from plants ...	2015	26483815
engineering modular viral scaffolds for targeted bacterial population editing.	bacteria are central to human health and disease, but existing tools to edit microbial consortia are limited. for example, broad-spectrum antibiotics are unable to accurately manipulate bacterial communities. bacteriophages can provide highly specific targeting of bacteria, but assembling well-defined phage cocktails solely with natural phages can be a time-, labor- and cost-intensive process. here, we present a synthetic-biology strategy to modulate phage host ranges by engineering phage genome ...	2015	26973885
bio-inspired electron-delivering system for reductive activation of dioxygen at metal centres towards artificial flavoenzymes.	development of artificial systems, capable of delivering electrons to metal-based catalysts for the reductive activation of dioxygen, has been proven very difficult for decades, constituting a major scientific lock for the elaboration of environmentally friendly oxidation processes. here we demonstrate that the incorporation of a flavin mononucleotide (fmn) in a water-soluble polymer, bearing a locally hydrophobic microenvironment, allows the efficient reduction of the fmn by nadh. this supramol ...	2015	26419885
molecular binding mechanism of ttgr repressor to antibiotics and antimicrobials.	a disturbing phenomenon in contemporary medicine is the prevalence of multidrug-resistant pathogenic bacteria. efflux pumps contribute strongly to this antimicrobial drug resistance, which leads to the subsequent failure of clinical treatments. the ttgr protein of pseudomonas putida is a hth-type transcriptional repressor that controls expression of the ttgabc efflux pump, which is the main contributor to resistance against several antimicrobials and toxic compounds in this microbe. one of the m ...	2015	26422008
assessing the antimicrobial activities of ocins.	the generation of a zone of inhibition on a solid substrate indicates the bioactivity of antimicrobial peptides such as bacteriocin and enterocin. the indicator strain plays a significant role in bacteriocin assays. other characteristics of bacteriocins, such as their dispersal ability and the different zymogram components, also affect bacteriocin assays. however, universal well diffusion assays for antimicrobials, irrespective of their ability to diffuse (bacteriocin and enterocin), do not exis ...	2015	26441952
identification of the gene cluster for the anaerobic degradation of 3,5-dihydroxybenzoate (α-resorcylate) in thauera aromatica strain ar-1.	thauera aromatica strain ar-1 degrades 3,5-dihydroxybenzoate (3,5-dhb) with nitrate as an electron acceptor. previous biochemical studies have shown that this strain converts 3,5-dhb to hydroxyhydroquinone (1,2,4-trihydroxybenzene) through water-dependent hydroxylation of the aromatic ring and subsequent decarboxylation, and they suggest a pathway homologous to that described for the anaerobic degradation of 1,3-dihydroxybenzene (resorcinol) by azoarcus anaerobius. southern hybridization of a t. ...	2015	26253674
genetically engineering bacillus subtilis with a heat-resistant arsenite methyltransferase for bioremediation of arsenic-contaminated organic waste.	organic manures may contain high levels of arsenic (as) due to the use of as-containing growth-promoting substances in animal feed. to develop a bioremediation strategy to remove as from organic waste, bacillus subtilis 168, a bacterial strain which can grow at high temperature but is unable to methylate and volatilize as, was genetically engineered to express the arsenite s-adenosylmethionine methyltransferase gene (cmarsm) from the thermophilic alga cyanidioschyzon merolae. the genetically eng ...	2015	26187966
transcriptional analysis of amorphotheca resinae zn1 on biological degradation of furfural and 5-hydroxymethylfurfural derived from lignocellulose pretreatment.	furfural and 5-hydroxymethylfurfural (hmf) are the two major inhibitor compounds generated from lignocellulose pretreatment, especially for dilute acid, steam explosion, neutral hot water pretreatment methods. the two inhibitors severely inhibit the cell growth and metabolism of fermenting strains in the consequent bioconversion step. the biodetoxification strain amorphotheca resinae zn1 has demonstrated its extraordinary capacity of fast and complete degradation of furfural and hmf into corresp ...	2015	26346604
three novel lantibiotics, ticins a1, a3, and a4, have extremely stable properties and are promising food biopreservatives.	lantibiotics are antimicrobial peptides with potential applications as the next generation of antimicrobials in the food industry and/or the pharmaceutical industry. nisin has successfully been used as a food preservative for over 40 years, but its major drawback is its limited stability under neutral and alkaline ph conditions. to identify alternatives with better biochemical properties, we screened more than 100 strains of the bacillus cereus group. three novel lantibiotics, ticins a1 (4,062.9 ...	2015	26231642
diversity of alkane hydroxylase genes on the rhizoplane of grasses planted in petroleum-contaminated soils.	the study investigated the diversity and genotypic features of alkane hydroxylase genes on rhizoplanes of grasses planted in artificial petroleum-contaminated soils to acquire new insights into the bacterial communities responsible for petroleum degradation in phytoremediation. four types of grass (cynodon dactylon, two phenotypes of zoysia japonica, and z. matrella) were used. the concentrations of total petroleum hydrocarbon effectively decreased in the grass-planted systems compared with the ...	2015	26405645
acinetobacter baumannii virulence is mediated by the concerted action of three phospholipases d.	acinetobacter baumannii causes a broad range of opportunistic infections in humans. its success as an emerging pathogen is due to a combination of increasing antibiotic resistance, environmental persistence and adaptation to the human host. to date very little is known about the molecular basis of the latter. here we demonstrate that a. baumannii can use phosphatidylcholine, an integral part of human cell membranes, as sole carbon and energy source. we report on the identification of three phosp ...	2015	26379240
the sociality of bioremediation: hijacking the social lives of microbial populations to clean up heavy metal contamination.		2015	26377978
the globally widespread genus sulfurimonas: versatile energy metabolisms and adaptations to redox clines.	sulfurimonas species are commonly isolated from sulfidic habitats and numerous 16s rrna sequences related to sulfurimonas species have been identified in chemically distinct environments, such as hydrothermal deep-sea vents, marine sediments, the ocean's water column, and terrestrial habitats. in some of these habitats, sulfurimonas have been demonstrated to play an important role in chemoautotrophic processes. sulfurimonas species can grow with a variety of electron donors and acceptors, which ...	2015	26441918
spontaneous phenotypic suppression of gaca-defective vibrio fischeri is achieved via mutation of csra and ihfa.	symbiosis defective gaca-mutant derivatives of vibrio fischeri are growth impaired thereby creating a selective advantage for growth-enhanced spontaneous suppressors. suppressors were isolated and characterized for effects of the mutations on gaca-mutant defects of growth, siderophore activity and luminescence. the mutations were identified by targeted and whole genome sequencing.	2015	26376921
c-type cytochrome assembly is a key target of copper toxicity within the bacterial periplasm.	in the absence of a tight control of copper entrance into cells, bacteria have evolved different systems to control copper concentration within the cytoplasm and the periplasm. central to these systems, the cu(+) atpase copa plays a major role in copper tolerance and translocates copper from the cytoplasm to the periplasm. the fate of copper in the periplasm varies among species. copper can be sequestered, oxidized, or released outside the cells. here we describe the identification of copi, a pe ...	2015	26396241
the influence of environmental conditions on kinetics of arsenite oxidation by manganese-oxides.	manganese-oxides are one of the most important minerals in soil due to their widespread distribution and high reactivity. despite their invaluable role in cycling many redox sensitive elements, numerous unknowns remain about the reactivity of different manganese-oxide minerals under varying conditions in natural systems. by altering temperature, ph, and concentration of arsenite we were able to determine how manganese-oxide reactivity changes with simulated environmental conditions. the interact ...	2015	26388696
exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in streptomyces.	there is a great demand for precisely quantitating the expression of genes of interest in synthetic and systems biotechnology as new and fascinating insights into the genetics of streptomycetes have come to light. here, we developed, for the first time to our knowledge, a quantitative method based on flow cytometry and a superfolder green fluorescent protein (sfgfp) at single-cell resolution in streptomyces. single cells of filamentous bacteria were obtained by releasing the protoplasts from the ...	2015	26374838
plant growth-promoting bacteria as inoculants in agricultural soils.	plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. plant growth-promoting bacteria (pgpb) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. several important bacterial characteristics, such as biological nitrogen fixation ...	2015	26537605
sulfoxides, analogues of l-methionine and l-cysteine as pro-drugs against gram-positive and gram-negative bacteria.	the problem of resistance to antibiotics requires the development of new classes of broad-spectrum antimicrobial drugs. the concept of pro-drugs allows researchers to look for new approaches to obtain effective drugs with improved pharmacokinetic and pharmacodynamic properties. thiosulfinates, formed enzymatically from amino acid sulfoxides upon crushing cells of genus allium plants, are known as antimicrobial compounds. the instability and high reactivity of thiosulfinates complicate their use ...	2015	26798500
an engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system.	biological containment is a genetic technique that programs dangerous organisms to grow only in the laboratory and to die in the natural environment. auxotrophy for a substance not found in the natural environment is an ideal biological containment. here, we constructed an escherichia coli strain that cannot survive in the absence of the unnatural amino acid 3-iodo-l-tyrosine. this synthetic auxotrophy was achieved by conditional production of the antidote protein against the highly toxic enzyme ...	2015	26401457
efficient recombinant production of prodigiosin in pseudomonas putida.	serratia marcescens and several other bacteria produce the red-colored pigment prodigiosin which possesses bioactivities as an antimicrobial, anticancer, and immunosuppressive agent. therefore, there is a great interest to produce this natural compound. efforts aiming at its biotechnological production have so far largely focused on the original producer and opportunistic human pathogen s. marcescens. here, we demonstrate efficient prodigiosin production in the heterologous host pseudomonas puti ...	2015	26441905
mechanistic implications of the unique structural features and dimerization of the cytoplasmic domain of the pseudomonas sigma regulator, pupr.	gram-negative bacteria tightly regulate intracellular levels of iron, an essential nutrient. to ensure this strict control, some outer membrane tonb-dependent transporters (tbdts) that are responsible for iron import stimulate their own transcription in response to extracellular binding by an iron-laden siderophore. this process is mediated by an inner membrane sigma regulator protein (an anti-sigma factor) that transduces an unknown periplasmic signal from the tbdt to release an intracellular s ...	2015	26313375
efflux systems in bacteria and their metabolic engineering applications.	the production of valuable chemicals from metabolically engineered microbes can be limited by excretion from the cell. efflux is often overlooked as a bottleneck in metabolic pathways, despite its impact on alleviating feedback inhibition and product toxicity. in the past, it has been assumed that endogenous efflux pumps and membrane porins can accommodate product efflux rates; however, there are an increasing number of examples wherein overexpressing efflux systems is required to improve metabo ...	2015	26363557
synergistic effect of pseudomonas putida and bacillus amyloliquefaciens ameliorates drought stress in chickpea (cicer arietinum l.).	two plant growth promoting rhizobacteria (pgpr) pseudomonas putida nbrira and bacillus amyloliquefaciens nbrisn13 with ability to tolerate abiotic stress along with multiple pgp traits like acc deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. earlier we have reported both the strains individually for their pgp attributes and stress amelioration in host pl ...	2015	26362119
synergistic effect of pseudomonas putida and bacillus amyloliquefaciens ameliorates drought stress in chickpea (cicer arietinum l.).	two plant growth promoting rhizobacteria (pgpr) pseudomonas putida nbrira and bacillus amyloliquefaciens nbrisn13 with ability to tolerate abiotic stress along with multiple pgp traits like acc deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. earlier we have reported both the strains individually for their pgp attributes and stress amelioration in host pl ...	2015	26362119
chassis optimization as a cornerstone for the application of synthetic biology based strategies in microbial secondary metabolism.	the increased number of bacterial genome sequencing projects has generated over the last years a large reservoir of genomic information. in silico analysis of this genomic data has renewed the interest in bacterial bioprospecting for bioactive compounds by unveiling novel biosynthetic gene clusters of unknown or uncharacterized metabolites. however, only a small fraction of those metabolites is produced under laboratory-controlled conditions; the remaining clusters represent a pool of novel meta ...	2015	26441855
benzoic acid-inducible gene expression in mycobacteria.	conditional expression is a powerful tool to investigate the role of bacterial genes. here, we adapt the pseudomonas putida-derived positively regulated xyls/pm expression system to control inducible gene expression in mycobacterium smegmatis and mycobacterium tuberculosis, the causative agent of human tuberculosis. by making simple changes to a gram-negative broad-host-range xyls/pm-regulated gene expression vector, we prove that it is possible to adapt this well-studied expression system to no ...	2015	26348349
2-hydroxy acids in plant metabolism.	glycolate, malate, lactate, and 2-hydroxyglutarate are important 2-hydroxy acids (2ha) in plant metabolism. most of them can be found as d- and l-stereoisomers. these 2ha play an integral role in plant primary metabolism, where they are involved in fundamental pathways such as photorespiration, tricarboxylic acid cycle, glyoxylate cycle, methylglyoxal pathway, and lysine catabolism. recent molecular studies in arabidopsis thaliana have helped elucidate the participation of these 2ha in in plant ...	2015	26380567
redox-switch regulatory mechanism of thiolase from clostridium acetobutylicum.	thiolase is the first enzyme catalysing the condensation of two acetyl-coenzyme a (coa) molecules to form acetoacetyl-coa in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel. here we elucidate the crystal structure of clostridium acetobutylicum thiolase (cathl) in its reduced/oxidized states. cathl, unlike those from other aerobic bacteria such as escherichia coli and zoogloea ramegera, is regulated by the redox-switch modulation through reversible disu ...	2015	26391388
an integrated insight into the response of sedimentary microbial communities to heavy metal contamination.	response of biological communities to environmental stresses is a critical issue in ecology, but how microbial communities shift across heavy metal gradients remain unclear. to explore the microbial response to heavy metal contamination (e.g., cr, mn, zn), the composition, structure and functional potential of sedimentary microbial community were investigated by sequencing of 16s rrna gene amplicons and a functional gene microarray. analysis of 16s rrna sequences revealed that the composition an ...	2015	26391875
effect of carbon on whole-biofilm metabolic response to high doses of streptomycin.	biofilms typically exist as complex communities comprising multiple species with the ability to adapt to a variety of harsh conditions. in clinical settings, antibiotic treatments based on planktonic susceptibility tests are often ineffective against biofilm infections. using a co2 evolution measurement system we delineated the real-time metabolic response in continuous flow biofilms to streptomycin doses much greater than their planktonic susceptibilities. stable biofilms from a multispecies cu ...	2015	26441887
effects of formulation on microbicide potency and mitigation of the development of bacterial insusceptibility.	risk assessments of the potential for microbicides to select for reduced bacterial susceptibility have been based largely on data generated through the exposure of bacteria to microbicides in aqueous solution. since microbicides are normally formulated with multiple excipients, we have investigated the effect of formulation on antimicrobial activity and the induction of bacterial insusceptibility. we tested 8 species of bacteria (7 genera) before and after repeated exposure (14 passages), using ...	2015	26253662
analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial bacillus amyloliquefaciens sqr9.	the plant growth-promoting rhizobacteria (pgpr) strain bacillus amyloliquefaciens sqr9, isolated from the cucumber rhizosphere, protects the host plant from pathogen invasion and promotes plant growth through efficient root colonization. the phytohormone indole-3-acetic acid (iaa) has been suggested to contribute to the plant-growth-promoting effect of bacillus strains. the possible iaa synthetic pathways in b. amyloliquefaciens sqr9 were investigated in this study, using a combination of chemic ...	2015	26337367
a novel and widespread class of ketosynthase is responsible for the head-to-head condensation of two acyl moieties in bacterial pyrone biosynthesis.	the biosynthesis of photopyrones, novel quorum sensing signals in photorhabdus, has been studied by heterologous expression of the photopyrone synthase ppys catalyzing the head-to-head condensation of two acyl moieties. the biochemical mechanism of pyrone formation has been investigated by amino acid exchange and bioinformatic analysis. additionally, the evolutionary origin of ppys has been studied by phylogenetic analyses also revealing homologous enzymes in pseudomonas sp. gm30 responsible for ...	2015	26425196
innovative biological approaches for monitoring and improving water quality.	water quality is largely influenced by the abundance and diversity of indigenous microbes present within an aquatic environment. physical, chemical and biological contaminants from anthropogenic activities can accumulate in aquatic systems causing detrimental ecological consequences. approaches exploiting microbial processes are now being utilized for the detection, and removal or reduction of contaminants. contaminants can be identified and quantified in situ using microbial whole-cell biosenso ...	2015	26322034
draft genome sequence of pseudomonas putida jlr11, a facultative anaerobic 2,4,6-trinitrotoluene biotransforming bacterium.	we report the draft genome sequence of pseudomonas putida jlr11, a facultative anaerobic bacterium that has been studied in detail for its capacity to use the explosive 2,4,6-trinitrotoluene (tnt) as a nitrogen source. the sequence confirms the mechanisms used by this versatile strain to reduce and assimilate nitrogen from tnt.	2015	26337875
molecular characterization of selected multidrug resistant pseudomonas from water distribution systems in southwestern nigeria.	persistence of antibiotic resistant bacteria, including multidrug resistant (mdr) pseudomonads, is an important environmental health problem associated with drinking water distribution systems (dwds) worldwide. there is paucity of data on the molecular characteristics of antibiotic resistance genes and their mode of transfer among pseudomonads from dwds located in resource-challenged areas such as southwestern nigeria.	2015	26328550
synthetic fatty acids prevent plasmid-mediated horizontal gene transfer.	bacterial conjugation constitutes a major horizontal gene transfer mechanism for the dissemination of antibiotic resistance genes among human pathogens. antibiotic resistance spread could be halted or diminished by molecules that interfere with the conjugation process. in this work, synthetic 2-alkynoic fatty acids were identified as a novel class of conjugation inhibitors. their chemical properties were investigated by using the prototype 2-hexadecynoic acid and its derivatives. essential featu ...	2015	26330514
complete biosynthesis of opioids in yeast.	opioids are the primary drugs used in western medicine for pain management and palliative care. farming of opium poppies remains the sole source of these essential medicines, despite diverse market demands and uncertainty in crop yields due to weather, climate change, and pests. we engineered yeast to produce the selected opioid compounds thebaine and hydrocodone starting from sugar. all work was conducted in a laboratory that is permitted and secured for work with controlled substances. we comb ...	2015	26272907
structural and functional adaptation of vancomycin resistance vant serine racemases.	vancomycin resistance in gram-positive bacteria results from the replacement of the d-alanyl-d-alanine target of peptidoglycan precursors with d-alanyl-d-lactate or d-alanyl-d-serine (d-ala-d-ser), to which vancomycin has low binding affinity. vant is one of the proteins required for the production of d-ala-d-ser-terminating precursors by converting l-ser to d-ser. vant is composed of two domains, an n-terminal membrane-bound domain, likely involved in l-ser uptake, and a c-terminal cytoplasmic ...	2015	26265719
whole-genome sequencing targets drug-resistant bacterial infections.	during the past two decades, the technological progress of whole-genome sequencing (wgs) had changed the fields of environmental microbiology and biotechnology, and, currently, is changing the underlying principles, approaches, and fundamentals of public health, epidemiology, health economics, and national productivity. today's wgs technologies are able to compete with conventional techniques in cost, speed, accuracy, and resolution for day-to-day control of infectious diseases and outbreaks in ...	2015	26243131
identification of a specific maleate hydratase in the direct hydrolysis route of the gentisate pathway.	in contrast to the well-characterized and more common maleylpyruvate isomerization route of the gentisate pathway, the direct hydrolysis route occurs rarely and remains unsolved. in pseudomonas alcaligenes ncimb 9867, two gene clusters, xln and hbz, were previously proposed to be involved in gentisate catabolism, and hbzf was characterized as a maleylpyruvate hydrolase converting maleylpyruvate to maleate and pyruvate. however, the complete degradation pathway of gentisate through direct hydroly ...	2015	26070679
phenotypic and phylogenetic identification of coliform bacteria obtained using 12 coliform methods approved by the u.s. environmental protection agency.	the current definition of coliform bacteria is method dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy of identification of true coliforms. this study used an alternative approach to the identification of true coliforms by combining the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16s rrna gene sequences with the use of lacz and uida genes. a collection of 1,404 isolates detected by 12 u.s. ...	2015	26116679
homogentisate 1-2-dioxygenase downregulation in the chronic persistence of pseudomonas aeruginosa australian epidemic strain-1 in the cf lung.	some pseudomonas aeruginosa strains including australian epidemic strain-1 (aes-1 or aus-01) cause persistent chronic infection in cystic fibrosis (cf) patients, with greater morbidity and mortality. factors conferring persistence are largely unknown. previously we analysed the transcriptomes of aes-1 grown in luria broth, nematode growth medium for caenorhabditis elegans assay (both aerobic) and artificial sputum medium (mainly hypoxic). transcriptional comparisons included chronic aes-1 strain ...	2015	26252386
high-level expression, purification and large-scale production of l-methionine γ-lyase from idiomarina as a novel anti-leukemic drug.	l-methionine γ-lyase (mgl), a pyridoxal 5'-phosphate-dependent enzyme, possesses anti-tumor activity. however, the low activity of mgl blocks the anti-tumor effect. this study describes an efficient production process for the recombinant mgl (rmgl) from idiomarina constructed using the overexpression plasmid in escherichia coli bl21 (de3), purification, and large-scale production. the enzyme produced by the transformants accounted for 53% of the total proteins and accumulated at 1.95 mg/ml using ...	2015	26308011
draft genome sequence of pseudomonas sp. strain lfm046, a producer of medium-chain-length polyhydroxyalkanoate.	pseudomonas sp. lfm046 is a medium-chain-length polyhydroxyalkanoate (phamcl) producer capable of using various carbon sources (carbohydrates, organic acids, and vegetable oils) and was first isolated from sugarcane cultivation soil in brazil. the genome sequence was found to be 5.97 mb long with a g+c content of 66%.	2015	26294616
draft genome sequence of the phenol-degrading bacterium pseudomonas putida h.	in this study, we report the draft genome of pseudomonas putida h, a well-known bacterium capable of degrading various aromatic compounds. its genome size is 6,065 mbp with a gc content of 61.6%. this work will aid future studies on this versatile bacterium.	2015	26294632
production of cinnamic and p-hydroxycinnamic acids in engineered microbes.	the aromatic compounds cinnamic and p-hydroxycinnamic acids (phcas) are phenylpropanoids having applications as precursors for the synthesis of thermoplastics, flavoring, cosmetic, and health products. these two aromatic acids can be obtained by chemical synthesis or extraction from plant tissues. however, both manufacturing processes have shortcomings, such as the generation of toxic subproducts or a low concentration in plant material. alternative production methods are being developed to enab ...	2015	26347861
salt stress induced changes in the exoproteome of the halotolerant bacterium tistlia consotensis deciphered by proteogenomics.	the ability of bacteria to adapt to external osmotic changes is fundamental for their survival. halotolerant microorganisms, such as tistlia consotensis, have to cope with continuous fluctuations in the salinity of their natural environments which require effective adaptation strategies against salt stress. changes of extracellular protein profiles from tistlia consotensis in conditions of low and high salinities were monitored by proteogenomics using a bacterial draft genome. at low salinity, w ...	2015	26287734
new delhi metallo-β-lactamase and oxa-48 carbapenemases in gram-negative bacilli isolates in libya.		2015	26294290
non-selective evolution of growing populations.	non-selective effects, like genetic drift, are an important factor in modern conceptions of evolution, and have been extensively studied for constant population sizes (kimura, 1955; otto and whitlock, 1997). here, we consider non-selective evolution in the case of growing populations that are of small size and have varying trait compositions (e.g. after a population bottleneck). we find that, in these conditions, populations never fixate to a trait, but tend to a random limit composition, and th ...	2015	26274606
combined endophytic inoculants enhance nickel phytoextraction from serpentine soil in the hyperaccumulator noccaea caerulescens.	this study assesses the effects of specific bacterial endophytes on the phytoextraction capacity of the ni-hyperaccumulator noccaea caerulescens, spontaneously growing in a serpentine soil environment. five metal-tolerant endophytes had already been selected for their high ni tolerance (6 mm) and plant growth promoting ability. here we demonstrate that individual bacterial inoculation is ineffective in enhancing ni translocation and growth of n. caerulescens in serpentine soil, except for specif ...	2015	26322074
a comparison of the microbial production and combustion characteristics of three alcohol biofuels: ethanol, 1-butanol, and 1-octanol.	over the last decade, microbes have been engineered for the manufacture of a variety of biofuels. saturated linear-chain alcohols have great potential as transport biofuels. their hydrocarbon backbones, as well as oxygenated content, confer combustive properties that make it suitable for use in internal combustion engines. herein, we compared the microbial production and combustion characteristics of ethanol, 1-butanol, and 1-octanol. in terms of productivity and efficiency, current microbial pl ...	2015	26301219
insect's intestinal organ for symbiont sorting.	symbiosis has significantly contributed to organismal adaptation and diversification. for establishment and maintenance of such host-symbiont associations, host organisms must have evolved mechanisms for selective incorporation, accommodation, and maintenance of their specific microbial partners. here we report the discovery of a previously unrecognized type of animal organ for symbiont sorting. in the bean bug riptortus pedestris, the posterior midgut is morphologically differentiated for harbo ...	2015	26324935
biophysics. comment on "extreme electric fields power catalysis in the active site of ketosteroid isomerase".	fried et al. (reports, 19 december 2014, p. 1510) demonstrate electric field-dependent acceleration of biological catalysis using ketosteroid isomerase as a prototypic example. these findings were not extended to aqueous solution because water by itself has field fluctuations that are too large and fast to provide a catalytic effect. given physiological context, when water electrostatic interactions are considered, electric fields play a less important role in the catalysis.	2015	26315427
phylogenetic analysis of methionine synthesis genes from thalassiosira pseudonana.	diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. the sulfate assimilation and methionine synthesis pathways provide s-containing amino acids for the synthesis of proteins and a range of metabolites such as dimethylsulfoniopropionate. to obtain an insight into the localization and organization of the sulfur metabolis ...	2015	26251775
co2 - intrinsic product, essential substrate, and regulatory trigger of microbial and mammalian production processes.	carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. as a consequence, [formula: see text] dissociation equilibria arise in fermenters by the growing culture. anaplerotic reactions make use of the abundant [formula: see text] levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. at the same time, co2 is released manifold in metabolic reactions via decarboxylation activity. the levels of ...	2015	26284242
biochemical and spectroscopic studies of epoxyqueuosine reductase: a novel iron-sulfur cluster- and cobalamin-containing protein involved in the biosynthesis of queuosine.	queuosine is a hypermodified nucleoside present in the wobble position of trnas with a 5'-gun-3' sequence in their anticodon (his, asp, asn, and tyr). the 7-deazapurine core of the base is synthesized de novo in prokaryotes from guanosine 5'-triphosphate in a series of eight sequential enzymatic transformations, the final three occurring on trna. epoxyqueuosine reductase (queg) catalyzes the final step in the pathway, which entails the two-electron reduction of epoxyqueuosine to form queuosine. ...	2015	26230193
rate-determining attack on substrate precedes rieske cluster oxidation during cis-dihydroxylation by benzoate dioxygenase.	rieske dearomatizing dioxygenases utilize a rieske iron-sulfur cluster and a mononuclear fe(ii) located 15 å across a subunit boundary to catalyze o2-dependent formation of cis-dihydrodiol products from aromatic substrates. during catalysis, o2 binds to the fe(ii) while the substrate binds nearby. single-turnover reactions have shown that one electron from each metal center is required for catalysis. this finding suggested that the reactive intermediate is fe(iii)-(h)peroxo or ho-fe(v)═o formed ...	2015	26154836
entner-doudoroff pathway for sulfoquinovose degradation in pseudomonas putida sq1.	sulfoquinovose (sq; 6-deoxy-6-sulfoglucose) is the polar head group of the plant sulfolipid sq-diacylglycerol, and sq comprises a major proportion of the organosulfur in nature, where it is degraded by bacteria. a first degradation pathway for sq has been demonstrated recently, a "sulfoglycolytic" pathway, in addition to the classical glycolytic (embden-meyerhof) pathway in escherichia coli k-12; half of the carbon of sq is abstracted as dihydroxyacetonephosphate (dhap) and used for growth, wher ...	2015	26195800
dna microarray-based identification of genes regulated by ntrc in bradyrhizobium japonicum.	the bradyrhizobium japonicum ntrbc two-component system is a critical regulator of cellular nitrogen metabolism, including the acquisition and catabolism of nitrogenous compounds. to better define the roles of this system, genome-wide transcriptional profiling was performed to identify the ntrc regulon during the response to nitrogen limitation. upon cells perceiving low intracellular nitrogen, they stimulate the phosphorylation of ntrc, which induces genes responsible for alteration of the core ...	2015	26025905
regulation mechanism of the ald gene encoding alanine dehydrogenase in mycobacterium smegmatis and mycobacterium tuberculosis by the lrp/asnc family regulator aldr.	in the presence of alanine, aldr, which belongs to the lrp/asnc family of transcriptional regulators and regulates ald encoding alanine dehydrogenase in mycobacterium smegmatis, changes its quaternary structure from a homodimer to an octamer with an open-ring conformation. four aldr-binding sites (o2, o1, o4, and o3) with a consensus sequence of ga/t-n2-nww/wwn-n2-a/tc were identified upstream of the m. smegmatis ald gene by means of dnase i footprinting analysis. o2, o1, and o4 are required for ...	2015	26195594
effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria.	enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ...	2015	26464607
permanent draft genome sequence of sulfoquinovose-degrading pseudomonas putida strain sq1.	pseudomonas putida sq1 was isolated for its ability to utilize the plant sugar sulfoquinovose (6-deoxy-6-sulfoglucose) for growth, in order to define its sq-degradation pathway and the enzymes and genes involved. here we describe the features of the organism, together with its draft genome sequence and annotation. the draft genome comprises 5,328,888 bp and is predicted to encode 5,824 protein-coding genes; the overall g + c content is 61.58 %. the genome annotation is being used for identificat ...	2015	27408681
development of a tightly controlled off switch for saccharomyces cerevisiae regulated by camphor, a low-cost natural product.	here we describe the engineering of a distant homolog of the tet repressor, camr, isolated from pseudomonas putida, that is regulated by camphor, a very inexpensive small molecule (at micromolar concentrations) for use in saccharomyces cerevisiae. the repressor was engineered by expression from a constitutive yeast promoter, fusion to a viral activator protein cassette, and codon optimization. a suitable promoter responsive to the camr fusion protein was engineered by embedding a p. putida opera ...	2015	26206350
isolation of extracellular polymeric substances from biofilms of the thermoacidophilic archaeon sulfolobus acidocaldarius.	extracellular polymeric substances (eps) are the major structural and functional components of microbial biofilms. the aim of this study was to establish a method for eps isolation from biofilms of the thermoacidophilic archaeon, sulfolobus acidocaldarius, as a basis for eps analysis. biofilms of s. acidocaldarius were cultivated on the surface of gellan gum-solidified brock medium at 78°c for 4 days. five eps extraction methods were compared, including shaking of biofilm suspensions in phosphat ...	2015	26380258
plastic waste as a novel substrate for industrial biotechnology.		2015	26482561
metabolic degradation of 1,4-dichloronaphthalene by pseudomonas sp. hy.	there is increasing concern regarding the adverse health effects of polychlorinated naphthalenes (pcns). the metabolic degradation of 1,4-dichloronaphthalene (1,4-dcn) as a model pcn, was studied using a strain of pseudomonas sp. hy. the metabolites were analyzed by gas chromatography-mass spectrometry (gc-ms). a series of metabolites including dihydroxy-dichloro-naphthalene, epoxy-dichlorinated naphthalene, dichlorinated naphthol, and dichlorinated salicylic acid were identified. the time-conce ...	2015	26308037
extracellular vesicles: role in inflammatory responses and potential uses in vaccination in cancer and infectious diseases.	almost all cells and organisms release membrane structures containing proteins, lipids, and nucleic acids called extracellular vesicles (evs), which have a wide range of functions concerning intercellular communication and signaling events. recently, the characterization and understanding of their biological role have become a main research area due to their potential role in vaccination, as biomarkers antigens, early diagnostic tools, and therapeutic applications. here, we will overview the rec ...	2015	26380326
functional identification of a novel gene, moae, for 3-succinoylpyridine degradation in pseudomonas putida s16.	microbial degradation of n-heterocyclic compounds, including xanthine, quinoline, nicotinate, and nicotine, frequently requires molybdenum hydroxylases. the intramolecular electron transfer chain of molybdenum hydroxylases consists of a molybdenum cofactor, two distinct [2fe-2s] clusters, and flavin adenine dinucleotide. 3-succinoylpyridine monooxygenase (spm), responsible for the transformation from 3-succinoylpyridine to 6-hydroxy-3-succinoylpyridine, is a crucial enzyme in the pyrrolidine pat ...	2015	26304596
analysis of the pathogenic potential of nosocomial pseudomonas putida strains.	pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. in this study we describe the multilocus sequence typing of four p. putida strains (hb13667, hb8234, hb4184, and hb3267) isolated from in-patients at the besançon hospital (france). the four isolates (in particular hb3267) were resistant to a number of antibiotics. the pathogenicity and virulence potential of the strains was tested ex ...	2015	26379646
pseudomonas aeruginosa iscr-regulated ferredoxin nadp(+) reductase gene (fprb) functions in iron-sulfur cluster biogenesis and multiple stress response.	p. aeruginosa (pao1) has two putative genes encoding ferredoxin nadp(+) reductases, denoted fpra and fprb. here, the regulation of fprb expression and the protein's physiological roles in [4fe-4s] cluster biogenesis and stress protection are characterized. the fprb mutant has defects in [4fe-4s] cluster biogenesis, as shown by reduced activities of [4fe-4s] cluster-containing enzymes. inactivation of the gene resulted in increased sensitivity to oxidative, thiol, osmotic and metal stresses compa ...	2015	26230408
removal of protein capping enhances the antibacterial efficiency of biosynthesized silver nanoparticles.	the present study demonstrates an economical and environmental affable approach for the synthesis of "protein-capped" silver nanoparticles in aqueous solvent system. a variety of standard techniques viz. uv-visible spectroscopy, transmission electron microscopy (tem), energy dispersive spectroscopy (eds) and x-ray diffraction (xrd) measurements were employed to characterize the shape, size and composition of nanoparticles. the synthesized nanoparticles were found to be homogenous, spherical, mon ...	2015	26226385
in vitro effect of bergamot (citrus bergamia) juice against caga-positive and-negative clinical isolates of helicobacter pylori.	helicobacter pylori infection has been associated with chronic gastritis, peptic ulcer and gastric carcinoma as over half of the world's population is colonized with this gram-negative bacterium. due to the increasing antibiotic resistance, its eradication rates fails in a great portion of patients. a number of studies showed that molecules largely distributed in commonly consumed fruits and vegetables may have antimicrobial activity. the aim of the present study was to investigate the effect of ...	2015	26220068
transcriptome profiling of wild-type and pga-knockout mutant strains reveal the role of exopolysaccharide in aggregatibacter actinomycetemcomitans.	exopolysaccharides have a diverse set of functions in most bacteria including a mechanistic role in protecting bacteria against environmental stresses. among the many functions attributed to the exopolysaccharides, biofilm formation, antibiotic resistance, immune evasion and colonization have been studied most extensively. the exopolysaccharide produced by many gram positive as well as gram negative bacteria including the oral pathogen aggregatibacter actinomycetemcomitans is the homopolymer of ...	2015	26221956
haloarchaea endowed with phosphorus solubilization attribute implicated in phosphorus cycle.	archaea are unique microorganisms that are present in ecological niches of high temperature, ph and salinity. a total of 157 archaea were obtained from thirteen sediment, water and rhizospheric soil samples collected from rann of kutch, gujarat, india. with an aim to screen phosphate solubilizing archaea, a new medium was designed as haloarchaea p solubilization (hps) medium. the medium supported the growth and p solubilization activity of archaea. employing the hps medium, twenty isolates showe ...	2015	26216440