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molecular properties of cis,cis-muconate cycloisomerase from pseudomonas putida. 19744449126
metabolism of biphenyl. structure and physicochemical properties of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, the meta-cleavage product from 2,3-dihydroxybiphenyl by pseudomonas putida.the structure of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid for the meta-cleavage product of 2,3-dihydroxybiphenyl by a pseudomonas putida strain was demonstrated on the basis of its chemical and physicochemical properties and those of its derivatives.19744462560
production of l-asparaginase by pseudomonas ovalis. 19744480497
proton magnetic resonance reveals high-spin iron (ii) in ferrous cytochrome p450 cam from pseudomonas putida.high-resolution proton nuclear magnetic resonance spectra were studied in d(+)-camphor-saturated solutions of ferric and ferrous cytochrome p450(cam) from pseudomonas putida, and of the cyanide complex of ferric p450(cam). in all these compounds several hyperfineshifted resonances of the heme group could be detected. in the ferrous protein, these resonance lines, which exhibit a curie-type temperature dependence in the range of 5-28 degrees , indicate the presence of high-spin iron (ii). it is s ...19724506075
genetic fusion of incompatible plasmids in pseudomonas.the genes specifying enzymes responsible for the degradation of camphor and octane occur on transmissible plasmids in pseudomonas putida strain ppg1 and p. oleovorans. since the presence of the plasmids is vital for the oxidative metabolism of camphor or octane (by the cells) in the absence of other carbon sources, such naturally occurring, energy-generating plasmids have been designated as degradative plasmids. the two degradative plasmids, cam and oct, are incompatible with each other and cann ...19734515925
magnetic circular dichroism studies. xxv. a preliminary investigation of microsomal cytochromes.the application of magnetic circular dichroism as an optical probe for simultaneous identification and determination of at least two microsomal cytochromes is demonstrated. the assignments of the bands in the spectra of microsomal suspensions are made from the spectra of soluble preparations of cytochrome p-450 obtained from pseudomonas putida and of cytochrome b(5) obtained from rat livers.19744521811
fusion and compatibility of camphor and octane plasmids in pseudomonas.the octane (oct) plasmid in pseudomonas putida derived from the omega-hydroxylase-carrying strain of coon and coworkers is transferable to the camphor (cam) plasmid-bearing strain by conjugation or by transduction. while the majority of the cam (+)oct(+) exconjugants segregate cam(+) or oct(+) cells, exconjugants with stable cam (+)oct(+) phenotype (cam-oct) can be detected at a low frequency. the transductants are all of the cam-oct phenotype. in the stable cam (+)oct(+) strains, the oct plasmi ...19744527812
a role of the putidaredoxin cooh-terminus in p-450cam (cytochrome m) hydroxylations.methylene hydroxylation by cytochrome p-450(cam) (cytochrome m) can be resolved into four distinct steps: substrate addition, m(o) --> m(os); reduction, m(os) --> m(rs); dioxygen addition, m(rs) --> m(o2) (rs); followed by a second putidaredoxin (pseudomonas putida ferredoxin)-mediated reduction and product formation. the isolated ferrous oxy-substrate complex exhibits first-order decay kinetics with the relatively slow rate constant of k [unk] 0.01 sec(-1), at 25 degrees , without product relea ...19744530269
dissociation and interaction of individual components of a degradative plasmid aggregate in pseudomonas.the transfer of the oct plasmid from pseudomonas oleovorans to pseudomonas putida strain ppgl results in the acquisition of three independent replicons: oct, factor k, and the mer plasmid. oct is a nontransmissible plasmid harboring genes that code for the enzymes responsible for the degradation of n-octane. factor k is a transfer plasmid capable of mobilizing oct as well as chromosomal genes but incapable of enhancing transfer frequencies of other transmissible plasmids such as cam, sal, or rp- ...19744530312
[evaluation of culture media used in the differentiation of pseudomonas aeruginosa, pseudomonas putida, and pseudomonas fluorescens, isolated from clinical human material]. 19744549846
kinetics of deoxyribonucleic acid destruction and synthesis during growth of bdellovibrio bacteriovorus strain 109d on pseudomonas putida and escherichia coli.during the growth of bdellovibrio bacteriovorus on pseudomonas putida or escherichia coli in either 10(-3)m tris(hydroxymethyl)aminomethane or in dilute nutrient broth, the host deoxyribonucleic acid (dna) was rapidly degraded, and by 30 to 60 min after the initiation of the bdellovibrio development cycle essentially all host dna became nonbandable in cscl gradients. at this stage the host dna degradation products were nondiffusable, and there was no appreciable pool of low-molecular-weight (col ...19724559819
isolation, identification, and characterization of a lipoate-degrading pseudomonad and of a lipoate catabolite.a strain of bacteria that can degrade lipoic acid was isolated from soil. the bacterium, adapted to use 0.4% dl-lipoate as the sole organic substrate to supply carbon, sulfur, and energy, was identified morphologically and physiologically as a strain of pseudomonas putida. degradation of 1,6-(14)c-lipoic acid, synthesized from 1,6-(14)c-adipic acid, was evidenced by: (i) loss of approximately 50% of the total radioactivity from the medium after bacterial growth; (ii) appearance of (14)c-degradat ...19724565525
genetic control of enzyme induction in the -ketoadipate pathway of pseudomonas putida: deletion mapping of cat mutations.a number of spontaneous mutant strains of pseudomonas putida, obtained by repeated selection for inability to grow with cis,cis-muconate, have been shown to carry deletions in catb, the structural gene for muconate lactonizing enzyme. these strains have been employed for deletion mapping of the genetic region containing catb and catc (the structural gene for muconolactone isomerase, the synthesis of which is coordinate with that of muconate lactonizing enzyme). all deletions that overlap mutant ...19724621687
genetic basis of the biodegradation of salicylate in pseudomonas.the genetic basis of the biodegradation of salicylate in pseudomonas putida r1 has been studied. this strain utilizes the meta pathway for oxidizing salicylate through formation of catechol and 2-hydroxymuconic semialdehyde. the enzymes of the meta pathway are induced by salicylate but not by catechol, and the genes specifying these enzymes are clustered. the gene cluster can be eliminated from some salicylate-positive cells by treatment with mitomycin c and appears to exist inside the cell as a ...19724628746
incidence and identification of pseudomonas fluorescens and pseudomonas putida in the clinical laboratory.strains of pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. a rapid nitrate test was found to be useful in distinguishing p. aeruginosa (positive) from p. fluorescens and p. putida (both negative). a shortened gelatin test differentiated p. fluorescens (positive) from p. putida (negative). p. fluorescens and p. putida were very sensitive to low levels of kanamycin and resistant to carbenicillin, a pattern just the oppo ...19734631431
the divergent meta-cleavage pathway for the metabolism of benzoate, 3-methylbenzoate and 4-methylbenzoate by pseudomonas arvilla mt-2. 19724634855
regulation of a catabolic pathway. lysine degradation in pseudomonas putida. 19724640470
camphor binding by pseudomonas putida cytochrome p-450. kinetics and thermodynamics of the reaction. 19724655251
purification and properties of 2-furoyl-coenzyme a hydroxylase from pseudomonas putida f2.1. 2-furoyl-coa hydroxylase of pseudomonas putida f2 has been purified 60-fold by a combination of (nh(4))(2)so(4) fractionation, deae-cellulose chromatography and agarose chromatography. 2. the purified enzyme catalyses the formation of 5-hydroxy-2-furoyl-coa, which tautomerizes to form 5-oxo-delta(2)-dihydro-2-furoyl-coa. 3. the enzyme has a requirement for an electron acceptor that can be satisfied by a membrane preparation from 2-furoate-grown ps. putida f2 or by artificial electron acceptor ...19724655411
stereospecific enzymes in the degradation of aromatic compounds by pseudomonas putida.two reactions in the catabolism of catechol by meta-fission, namely, hydration of 2-oxopent-4-enoate (vinylpyruvate) and aldol fission of the product, are catalyzed by stereospecific enzymes. the absolute configuration of this hydration product was shown to be l(s)-4-hydroxy-2-oxopentanoate. vinylpyruvate hydratase, purified almost to homogeneity, had a molecular weight of about 287,000 and was dissociated in sodium dodecyl sulfate, without prior treatment with mercaptoethanol, into a species wi ...19734690969
anthranilate synthase from pseudomonas putida. purification and properties of a two-component enzyme. 19734692828
initial reactions in the oxidation of ethylbenzene by pseudomonas putida. 19734699984
absolute stereochemistry of the (+)-cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene produced from toluene by pseudomonas putida. 19734710064
transmissible plasmid coding early enzymes of naphthalene oxidation in pseudomonas putida.the capacity of pseudomonas putida ppg7 (atcc 17,485) to grow on naphthalene, phenotype nah(+), is lost spontaneously, and the frequency is increased by treatment with mitomycin c. the nah(+) growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. after mutagenesis, strains can be selected with increased donor capacity in conjugation. clones which use naphthalene grow on s ...19734712575
pseudomonas putida and septic arthritis. 19734713580
benzoate metabolism in pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways.benzoate-grown cells of pseudomonas putida(arvilla) mt-2 contain both metapyrocatechase and pyrocatechase activities, although the former activity is much higher than that of the latter. a spontaneous mutant deficient in metapyrocatechase and 2-hydroxymuconic semialdehyde hydrolyase, the first two enzymes in the meta-cleavage pathway of the ring of catechol, has been isolated from this strain. this mutant grows well on a minimal medium containing benzoate as a sole carbon source and has the high ...19734717515
microbial metabolism of amino alcohols. 1-aminopropan-2-ol and ethanolamine metabolism via propionaldehyde and acetaldehyde in a species of pseudomonas.1. growth and manometric experiments showed that a pseudomonas sp. p6 (n.c.i.b. 10431), formerly known as achromobacter sp. p6, was capable of growth on both stereoisomers of 1-aminopropan-2-ol, and supported the hypothesis that assimilation involved metabolism to propionaldehyde, propionate and possibly 2-hydroxyglutarate. a number of alternative intermediary metabolites were ruled out. 2. accumulation of propionaldehyde from 1-aminopropan-2-ol by intact cells occurred only during active growth ...19734723219
glucolysis in pseudomonas putida: physiological role of alternative routes from the analysis of defective mutants.a number of mutants in which glucolysis is impaired have been isolated from pseudomonas putida. the study of their behavior shows that this organism possesses a single glucolytic pathway with physiological significance. the first step of the pathway consists in the oxidation of glucose into gluconate. two proteins with glucose dehydrogenase activity appear to exist in p. putida but the reasons for this duplicity are not clear. the process continues with the formation of 2-ketogluconate which is ...19734745434
camphor plasmid-mediated chromosomal transfer in pseudomonas putida.camphor-utilizing strains of pseudomonas putida have been shown to carry the genetic information required for camphor degradation on a plasmid. the plasmid-carrying strains can serve as donors of both plasmid-borne and chromosomal genes. as recipients, plasmid-deleted strains are much superior to those carrying the camphor pathway genes. the transfer frequency of chromosomal, but not plasmid-borne, genes is markedly enhanced if the donor cells are irradiated with ultraviolet light followed by 3- ...19734745436
metabolism of biphenyl. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate: the meta-cleavage product from 2,3-dihydroxybiphenyl by pseudomonas putida.1. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid was isolated and identified from washed suspensions of pseudomonas putida incubated in the presence of 2,3-dihydroxybiphenyl. 2. benzoic acid was isolated from reaction mixtures of crude cell-free extracts incubated with 2,3-dihydroxybiphenyl. 3. the presence in the same reaction mixtures of either 4-hydroxy-2-oxovalerate or 2-hydroxypenta-2,4-dienoate was suggested by mass spectrometry. 4. the degradative pathway of biphenyl is discussed.19734762751
regulation of the glucolytic enzymes in pseudomonas putida. 19734764724
transformation of o-toluate in pseudomonas putida isolate 1065 and rhizopus japonicus atcc 24794. 19734775413
[activation of fatty acids by an enzyme system extracted from pseudomonas putida]. 19734793588
growth of pseudomonas eisenbergii in activated sludges of water-treatment plants. 19734802488
the clustering on the pseudomonas putida chromosome of genes specifying dissimilatory functions. 19734807194
role of catechol and the methylcatechols as inducers of aromatic metabolism in pseudomonas putida.pseudomonas putida ncib 10015 metabolizes phenol and the cresols (methylphenols) by the meta pathway and metabolizes benzoate by the ortho pathway. growth on catechol, an intermediate in the metabolism of both phenol and benzoate, induces both ortho and meta pathways; growth on 3- or 4-methylcatechols, intermediates in the metabolism of the cresols, induces only the meta pathway to a very limited degree. addition of catechol at a growth-limiting rate induces virtually no meta pathway enzymes, bu ...19744813893
purification and characterization of a b6-independent threonine dehydratase from pseudomonas putida. 19744814721
dissociation of a degradative plasmid aggregate in pseudomonas.the infectious plasmid oct, which specifies a set of dissimilatory enzymes responsible for the degradation of n-octane, has been shown to be an aggregate of a noninfectious oct plasmid and an infectious plasmid with sex factor activity. the infectious plasmid, which can be eliminated from the cells of pseudomonas putida by mitomycin c treatment without loss of the oct plasmid and vice versa, has been designated as factor k. the infectious plasmid (factor k) is not only responsible for the mobili ...19744829926
new oxygenases in the degradation of flavones and flavanones by pseudomonas putida. 19744831363
transport of succinate by pseudomonas putida. 19744831622
mandelate racemase from pseudomonas putida. subunit composition and absolute divalent metal ion requirement. 19744831901
mandelate racemase from pseudomonas putida. affinity labeling of the enzyme by d,l-alpha-phenylglycidate in the presence of magnesium ion. 19744831902
the amino acid sequence of putidaredoxin, an iron-sulfur protein from pseudomonas putida. 19744833743
a 4-methoxybenzoate monooxygenase system from pseudomonas putida. circular dichroism studies on the iron--sulfur protein. 19744850360
bacterial metabolism of para- and meta-xylene: oxidation of the aromatic ring.pseudomonas putida 39/d oxidized p-xylene to cis-3,6-dimethyl-3,5-cyclohexadiene-1,2-diol (cis-p-xylene dihydrodiol). the latter compound was isolated in crystalline form and its physical properties were determined. the cis configuration of the hydroxyl groups in the oxidation product was inferred from its ability to form an isopropylidene derivative with 2,2-dimethoxypropane. acid treatment of cis-p-xylene dihydrodiol resulted in the formation of 2,5-dimethylphenol. a partially purified prepara ...19744850728
the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria.1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ...19694886765
herellea (acinetobacter) and pseudomonas ovalis (p. putida) from frozen foods.seventeen strains of herellea vaginicola (acinetobacter antitratus) and 8 of pseudomonas ovalis (p. putida), isolated from 23 (6.3%) of 364 samples of frozen, foil-pack foods, were identified and characterized morphologically and biochemically. herellea was isolated from 17 foods (4.7%), p. ovalis from 6 (1.6%). no mima were found. the food samples included precooked frozen meats, precooked and uncooked frozen vegetables, and uncooked frozen desserts. the bacteria were detected in the food with ...19694886860
2'-o-methyladenosine 5'-triphosphate. a substrate for deoxyribonucleic acid dependent ribonucleic acid polymerase of pseudomonas putida. 19714935104
pseudomonas putida tryptophan synthetase: partial sequence of the subunit.there is 50% identity in the sequences of the first 50 residues of the alpha chains of escherichia coli and pseudomonas putida. no deletions or additions of residues are found in this region, except for the n-terminal methionine residue which is missing in the polypeptide isolated from p. putida. most of the residues which differ are chemically dissimilar, and half of them are specified by codons which differ by more than a single base. the two residues known by mutational analysis to be essenti ...19714941557
pseudomonas putida tryptophan synthetase.the two protein components of pseudomonas putida tryptophan synthetase have been purified to homogeneity. although there is general similarity between the pseudomonas enzyme and that of the enteric bacteria, many differences were found. components from escherichia coli and p. putida do not stimulate each other enzymatically, and the enzymes differ in their response to monovalent cations. serine deamination occurs best with the intact enzyme of p. putida, not with the beta(2) subunit alone as in ...19714941565
comparative immunological studies of two pseudomonas enzymes.crystalline preparations of muconate lactonizing enzyme and muconolactone isomerase, two inducible enzymes that catalyze successive steps in the catechol branch of the beta-ketoadipate pathway, were used to prepare antisera. both enzymes were isolated from a strain of pseudomonas putida biotype a. the antisera did not cross-react with enzymes of the same bacterial strain that catalyze the chemically analogous steps in the protocatechuate branch of the beta-ketoadipate pathway, carboxymuconate la ...19704986759
mandelic acid racemase from pseudomonas putida. purification and properties of the enzyme. 19704989844
[fine structure of the wall of a pseudomonas putida]. 19714997330
aerobic metabolism of -amino-n-butyric acid by pseudomonas putida. 19714999027
properties and subunit structure of the b component of pseudomonas putida tryptophan synthetase. 19715000700
pathogenic significance of pseudomonas fluorescens and pseudomonas putida. 19715002396
[physiology of fluorescent pseudomonas and characteristics of pseudomonas putida]. 19715005193
template activity of 2'-o-methylpolyribonucleotides with pseudomonas putida dna-dependent rna polymerase. 19725012160
release of the sigma subunit of pseudomonas putida deoxyribonucleic acid dependent ribonucleic acid polymerase. 19725013820
metabolism of basic amino acids in pseudomonas putida. transport of lysine, ornithine, and arginine. 19725019949
photoactivation of urocanase in pseudomonas putida: action spectrum. 19725021838
regulation of valine catabolism in pseudomonas putida.the activities of six enzymes which take part in the oxidation of valine by pseudomonas putida were measured under various conditions of growth. the formation of four of the six enzymes was induced by growth on d- or l-valine: d-amino acid dehydrogenase, branched-chain keto acid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, and methylmalonate semialdehyde dehydrogenase. branched-chain amino acid transaminase and isobutyryl-coa dehydrogenase were synthesized constitutively. d-amino acid dehy ...19725030618
catabolism of pipecolate to glutamate in pseudomonas putida. 19725033403
metabolism of basic amino acids in pseudomonas putida. -guanidinobutyrate amidinohydrolase. 19725043851
carbon monoxide binding by pseudomonas putida cytochrome p-450. 19725045928
photoactivation of urocanase in pseudomonas putida: possible conformational change during activation. 19725051931
inducible uptake system for -carboxy-cis, cis-muconate in a permeability mutant of pseudomonas putida.a procedure for the large-scale enzymatic synthesis of beta-carboxymuconate is described. when used as a growth substrate, beta-carboxymuconate selected for mutant strains of pseudomonas putida that were permeable to polycarboxylic acid intermediates of the beta-ketoadipate pathway. one mutant organism, strain prs2110, was investigated in detail. it differed from the parental strain in that it possessed a beta-carboxymuconate uptake system that was formed when the compound was supplied exogenous ...19725053469
genetic control of enzyme induction in the -ketoadipate pathway of pseudomonas putida: two-point crosses with a regulatory mutant strain.several mutant strains of pseudomonas putida, selected on the basis of their inability to grow at the expense of benzoate, have been shown to be unable to form inducibly both muconate lactonizing enzyme and muconolactone isomerase. a secondary mutant strain derived from one of these pleiotropically negative strains forms these two enzymes and, in addition, catechol oxygenase in the absence of inducer. this constitutive mutant strain was used as a donor in transductionally mediated two-point cros ...19725058453
regulation of pathways degrading aromatic substrates in pseudomonas putida. enzymic response to binary mixtures of substrates.1. induction constants (k(ind)) and repression constants (k(rep)), which are a measure of the affinity of the inducers or repressors for the induction systems, were measured for mandelate, benzoate and p-hydroxybenzoate in pseudomonas putida. 2. from these results, the enzymic response of the organism to media containing pairs of these substrates was predicted. nitrogen-limited chemostats, operated at high growth rates, were used to investigate these predictions in cells grown first on one aroma ...19725073241
evidence for induced synthesis of an active transport factor for mandelate in pseudomonas putida.1. at low concentrations of mandelate there is a lag before induction of the mandelate regulon begins at a sub-maximum rate. cells preinduced with a saturating concentration of inducer do not exhibit this lag when they are transferred to sub-maximum inducer concentrations and are able to maintain a high rate of induction under these conditions. 2. chloramphenicol was used to show that a protein is synthesized during the lag period that is probably responsible for the maintenance effects observed ...19725073242
deoxyribonucleic acid-dependent ribonucleic acid polymerase of pseudomonas putida. 19715101769
purification and kinetic properties of aconitate isomerase from pseudomonas putida. 19715114987
apo- and reconstituted holoenzymes of metapyrocatechase from pseudomonas putida. 19715127383
the metabolism of biphenyl by pseudomonas putida. 19715127867
induction of separate catabolic pathways for l- and d-lysine in pseudomonas putida. 19715128165
tryptophan synthetase 2 subunit. primary structure of the pyridoxyl peptide from the pseudomonas putida enzyme. 19715132675
enzymatic production of l-citrulline by pseudomonas putida.to develop an efficient method for the production of l-citrulline, optimum conditions for the conversion of l-arginine to l-citrulline by microbial l-arginine deiminase and for production of the enzyme were studied. a number of micro-organisms were screened to test their ability to form and accumulate l-citrulline from l-arginine. pseudomonas putida was selected as the best organism. with this organism, enzyme activity as high as 9.20 units per ml could be produced by a shaking culture at 30 c i ...19715137589
some studies on the purified 2-furoyl-coenzyme a hydroxylase from pseudomonas putida f2. 19715144719
inducibility of tryptophan synthetase in pseudomonas putida. 19665229989
defective phage and chromosome mobilization in pseudomonas putida.the transfer of transducing phage dna in association with the mandelate genetic region of pseudomonas putida strain prs1 (termed pfdm) has been achieved by growing together mandelate-positive ppg2 cells harboring pfdm as an extrachromosomal element and mandelate-deleted ppg1 strains. this transfer is analogous to sexual conjugation in the enterobacteria. the transfer of pfdm elements is always associated with chromosome mobilization and some rare recombinants acquire genetic donor ability. we ha ...19695271748
anthranilate synthase enzyme system and complementation in pseudomonas species.anthranilate synthase in pseudomonas putida is a two component enzyme system. the proteins, termed as i and as ii, have respective molecular weights of 65,000 and 18,000. five additional pseudomonas species, both tryptophan requiring and independent strains, were examined and all were shown to contain similar two component systems. anthranilate formation by "amide transfer," with l-glutamine as nitrogen donor, requires both proteins; "amination," utilizing ammonium ion, proceeds at ph 9 with onl ...19705274451
the conversion of catechol and protocatechuate to beta-ketoadipate by pseudomonas putida. 3. enzymes of the catechol pathway. 19665330966
alpha-hydroxyglutarate oxidoreductase of pseudomonas putida.oxidation of d-alpha-hydroxyglutarate to alpha-ketoglutarate is catalyzed by d-alpha-hydroxyglutarate oxidoreductase, an inducible membrane-bound enzyme of the electron transport particle [etp; a comminuted cytoplasmic membrane preparation with enzymic properties and chemical composition resembling beef heart mitochondrial etp (1)] of pseudomonas putida p2 (p2-etp). treatment of p2-etp with a nonionic detergent yields a preparation with the sedimentation characteristics of a soluble enzyme, but ...19695354943
phenol and benzoate metabolism by pseudomonas putida: regulation of tangential pathways.catechol occurs as an intermediate in the metabolism of both benzoate and phenol by strains of pseudomonas putida. during growth at the expense of benzoate, catechol is cleaved ortho (1,2-oxygenase) and metabolized via the beta-ketoadipate pathway; during growth at the expense of phenol or cresols, the catechol or substituted catechols formed are metabolized by a separate pathway following meta (2,3-oxygenase) cleavage of the aromatic ring of catechol. it is possible to explain the mutually excl ...19695354952
regulation of the meta cleavage pathway for benzoate oxidation by pseudomonas putida.catechol or 2-hydroxymuconic semialdehyde cannot participate as functional inducers of the meta pathway for benzoate metabolism in pseudomonas putida. induction of the first two enzymes of the pathway must be mediated by benzoate, or its analogues, as primary substrate.19695359614
identification of alpha-ketobutyrate as the prosthetic group of urocanase from pseudomonas putida. 19705412708
biosynthesis of ubiquinone in non-photosynthetic gram-negative bacteria.1. the polyprenylphenol and quinone complements of the non-photosynthetic gram-negative bacteria, pseudomonas ovalis chester, proteus mirabilis and ;vibrio o1' (moraxella sp.), were investigated. 2. ps. ovalis chester and prot. mirabilis were shown to contain 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 5-demethoxyubiquinones, ubiquinones, an unidentified 1,4-benzoquinone [2-polyprenyl-1,4-benzoquinone (?)] and ;epoxyubiquinones'. ;vibrio o1' was ...19705420949
photoactivation of urocanase in pseudomonas putida: possible role in photoregulation of histidine metabolism.irradiation by near-ultraviolet light of cells or extracts of pseudomonas putida increased the urocanase activity. irradiated cells exhibited enhanced catabolic activity on histidine and urocanate.19705429726
delta-aminovaleramidase of pseudomonas putida. 19705432799
incorporation of oxygen-18 into benzene by pseudomonas putida. 19705436152
properties of the inducible hydroxyproline transport system of pseudomonas putida.features of the transport system for hydroxyproline in a strain of pseudomonas putida were studied. a mutant, lacking hydroxyproline-2 epimerase and unable to metabolize hydroxy-l-proline, was shown to transport and accumulate this compound after induction. both entry and exit rates were examined, and kinetic constants for the reaction were determined. increasing the induction time from 0.5 to 3 hr increased the entry rate three- to fourfold but had only a small and variable effect on the exit r ...19705438054
the aspartate kinase of pseudomonas putida. regulation of synthesis and activity. 19705441401
homoserine dehydrogenase of pseudomonas putida. properties and regulation. 19705441405
the purification and characterization of adenosine triphosphate ribonucleic acid adenyltransferase from pseudomonas putida. 19705442825
mandelic acid racemase from pseudomonas putida. evidence favoring a carbanion intermediate in the mechanism of action. 19705458641
regulation of synthesis of early enzymes of p-hydroxybenzoate pathway in pseudomonas putida. 19705469168
oxidation of furan-2-carboxylate to 2-oxoglutarate by pseudomonas putida f2: studies of enzymology and electron transport. 19705472188
biosynthesis of amino acids from hydrocarbons. 3. determination of optimum conditions for pseudomonas arvilla. 19705515453
the genetic control of dissimilatory pathways in pseudomonas putida. 19705525301
purification and characterization of fumarase from pseudomonas putida. 19705529637
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