bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from pseudomonas putida orc.the hydroxylase activities observed in extracts of pseudomonas putida orc after growth on orcinol and resorcinol as sole source of carbon have been purified to homogeneity. both enzymes were shown to be flavoproteins and to contain approximately 1 mol of fad for each polypeptide chain, s20,w values for each enzyme are 4.1 +/- 0.1 and are independent of the presence of their aromatic substrates. molecular weight determinations under native (approximately 68000) and denaturing (approximately 70000 ...19761280
crystalline aspartate aminotransferase from pseudomonas striata. 19761290
cytochrome p450cam and its complexes. mössbauer parameters of the heme iron.mössbauer spectroscopy has been used to study the heme iron in various states of cytochrome p450cam from the camphor-hydroxylating system of the bacterium pseudomonas putida. native, camphor-free p450cam contains low-spin ferric iron, part of which (approx. 50-70%) is converted to the high-spin ferric state upon addition of camphor. the mössbauer spectra of the camphor-free enzyme (s equals 1/2) and of the high-spin component (s equals 5/2) of the camphor complex have been successfully simulated ...19762296
d-alpha-hydroxyglutarate dehydrogenase of rhodospirillum rubrum.d-alpha-hydroxyglutarate dehydrogenase of r. rubrum grown anaerobically in the light was partially purified and some properties were investigated. 1. the enzyme catalyze stoichiometrically the dehydrogenation reaction of d-alpha-hydroxyglutarate into alpha-oxoglutarate, coupled with the reduction of 2, 6-dichlorophenolindophenol. 2. cytochrome c2, cytochrome c, and ferricyanide are effective as electron acceptors with the crude enzyme but not with the purified one, whereas nad+ and nadp+ are com ...19755424
purification and characterization of methioninase from pseudomonas putida.methioninase of pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. the purified enzyme had an s20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. a break in the arrhenius plot was observed at 40 degrees and the activation energies below and above this temperature were 15.5 and 2.97 kcal per mole, respectively. 3. in addition to l-methionine, vari ...19768440
purification, crystallization, and some properties of creatine amidinohydrolase from pseudomonas putida.a method was developed for purification and crystallization of creatinase creatine amidinohydrolase, ec from pseudomonas putida var. naraensis c-83. the purified preparation appeared homogeneous on disc electrophoresis and ultracentrifugation and had a molecular weight of 94,000. it was most active at ph 8 and stable between ph 6 and 8 for 24 hr at 37 degrees. sds-polyacrylamide gel electrophoresis indicated that the native enzyme was made up of two subunit monomers, the molecular weight ...19768443
purifications and properties of l-mandelate- 4-hydroxylase from pseudomonas convexa. 19769909
properties of iron-sulfur protein isolated from pseudomonas ovalis. 197610907
serum bactericidal activity in the horseshoe crab, limulus polyphemus.serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ...197611188
kinetics and properties of l-glutaminase and l-asparaginase activities of pseudomonas ovalis.pseudomonas ovalis produces l-glutaminase and l-asparaginase activities simultaneously upon induction by l-glutamine or l-asparagin in the growth medium. both activities are confined to the cell during active growth and are not released into the medium. the apparent km values are 1.4 x 10(-2) m and 6 x 10(-3) m for l-glutamine and l-asparagine substrates, respectively. induction of both activities is substantially favoured in media with initial ph values higher than 7. in buffered yeast extract ...197613588
exchange reactions catalyzed by methioninase from pseudomonas putida. isolation and characterization of the exchange products.gel-electrophoretically homogeneous methioninase l-methionine methanethiol-lyase (deaminating), ec of pseudomonas putida, which catalyzes alpha, beta- and alpha, gamma-eliminations from s-substituted amino acids, could also catalyze a variety of beta- and gamma-exchange reactions, according to the following equations: rsch2ch(nh2)cooh+r'sh in equilibrium r'sch2ch(nh2)cooh+rsh (beta-exchange) and rsch2ch2ch(nh2)cooh+r'sh in equilibrium r'sch2ch2ch(nh2)cooh+rsh (gamma-exchange), where r's ...197614124
purification and properties of cis-toluene dihydrodiol dehydrogenase from pseudomonas putida.the purification of (+)-cis-1(s),2(r)-dihydroxy-3-methylcyclohexa-3,5-diene dehydrogenase from cells of pseudomonas putida grown with toluene as the sole source of carbon and energy is reported. the molecular weight of the enzyme is 104,000 at ph 9.7. the enzyme is composed of four apparently identical subunits with molecular weights of 27,000. the enzyme is specific for nicotinamide adenine dinucleotide and oxidizes a number of cis-dihydrodiols. both enantiomers of a racemic mixture of cis-1,2- ...197716865
properties of 1-phosphofructokinase from pseudomonas putida.the 1-phosphofructokinase (1-pfk, ec from pseudomonas putida was partially purified by a combination of (nh4)2so4 fractionation and deae-sephadex column chromatography. in its kinetic properties, this enzyme resembled the 1-pfk's from other bacteria. with the substrates fructose-1-phosphate (f-1-p) and adenosine triphosphate (atp) michaelis-menten kinetics were observed, the km for one substrate being unaffected by a variation in the concentration of the other substrate. at ph 8.0, the ...197717464
the purification and properties of 4-hydroxyisophthalate hydroxylase from pseudomonas putida ncib 9866. 197718349
presence of tightly bound nad+ in urocanase of pseudomonas putida. 197718470
comparison of two dioxygenases from pseudomonas putida.catechol 2,3-dioxygenase and homoprotocatechuate 2,3-dioxygenase were purified from the same strain of pseudomonas putida. molecular weights and subunit sizes were similar, but amino acid compositions showed some marked differences.197719416
photoactivation of urocanase in pseudomonas putida. temperature-compensated in vitro model of an hourglass timer. 197720925
a study on the reconstitution of iron-superoxide dismutase from pseudomonas ovalis. 197825273
the purification and properties of urocanase from pseudomonas testosteroni.urocanase (urocanate hydratase, ec purified from pseudomonas testosteroni has a mol.wt. of 118000 determined by sedimentation-equilibrium analysis. ultracentrifugation in 6m-guanidine hydrochloride and polyacrylamide-gel electrophoresis in sodium dodecyl sulphate show that the enzyme consists of two identical or very similar subunits. it is, like urocanase isolated from other sources, inhibited by reagents that react with carbonyl groups. although urocanase from ps. testosteroni is str ...197825660
purification and characterization of a heme-containing amine dehydrogenase from pseudomonas putida.the primary amine dehydrogenase of pseudomonas putida np was purified to homogeneity as judged by polyacrylamide gel electrophoresis. cytochrome c or an artificial electron acceptor was required for amine dehydrogenase activity. the enzyme was nonspecific, readily oxidizing primary monoamines, benzylamine, and tyramine; little or no measurable activity was detected with isoamines, l-ornithine, l-lysine, and certain diamines or polyamines. the ph optima for n-butylamine, benzylamine, and n-propyl ...197826667
photoactivation of urocanase in pseudomonas putida. role of sulfite in enzyme modification. 197829899
identification of the prosthetic group of urocanase. the mode of its reaction with sodium borohydride and of its photochemical reactivation.urocanase from pseudomonas putida and from beef liver were isolated by modifying described procedures. both enzymes were inactivated and labeled on treatment with tritiated sodium borohydride and gave, upon subsequent hydrolysis, a radioactive acid. the previously reported identity of this acid as 2-hydroxybutanoic acid was disproved by several criteria. other hydroxy acids were also proved to be different from the radioactive acid derived from urocanase. a large portion of the radioactive mater ...197933176
immunological study of anthranilate immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ...197550316
bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.cells of pseudomonas putida np, pseudomonas species (ncib 9816), and a nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (nad+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. similarly, cells of a strain of p. putida biotype a, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis ste ...197662750
[functional irreversibility of the anabolic ornithine carbamyltransferase in pseudomonas putida. kinetic analysis]. 197664201
immunological comparison of enzymes of the beta-ketoadipate pathway.beta-carboxy-cis,cis-muconate lactonizing enzyme and gamma-carboxymuconolactone decarboxylase catalyze sequential reactions in the beta-ketoadipate pathway; the subunit sizes of the enzymes from pseudomonas putida, biotype a, are 40 000 and 13 000, respectively. the cross reaction of antisera prepared against the enzymes was tested with the isofunctional enzymes formed by representatives of other bacterial species. despite the differences in the subunit sizes of the enzymes, the antisera reveale ...197665161
purification and properties of 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase from two strains of pseudomonas putida.growth on phenol of two strains of pseudomonas putida biotype a, ncib 10015 and ncib 9865, elicits the synthesis of an enzyme that hydrolyzes 2-hydroxy-6-oxo-2,4-heptadienoate to 2-oxopent-4-enoate. the purified enzyme from pseudomonas ncib 10015 has a molecular weight of 118,000 and dissociates in sodium dodecyl sulfate to a species of molecular weight 27,700; the enzyme from pseudomonas ncib 9865 has a molecular weight of 100,000 and dissociates to a species of 25,000 molecular weight. the hyd ...197877272
isolation of large bacterial plasmids and characterization of the p2 incompatibility group plasmids pmg1 and pmg5.large plasmids from agrobacterium tumefaciens, salmonella typhimurium, escherichia coli, pseudomonas putida, and pseudomonas aeruginosa were routinely and consistently isolated using a procedure which does not require ultracentrifugation but includes steps designed to separate large-plasmid dna from the bacterial folded chromosome. it also selectively removes fragments of broken chromosome. a variety of large plasmids was readily visualized with agarose gel electorphoresis, including five betwee ...197897269
tol is a broad-host-range plasmid.we readily isolated insertions of the carbenicillin resistance element tn401 into the tol plasmid in pseudomonas putida. hybrid tol::tn401 plasmids stably express the cbr phenotype in pseudomonas aeruginosa and escherichia coli. whereas the replicative and conjugative functions are expressed in both hosts, the ability to grow on m-toluate is only expressed in the pseudomonas species.197897271
[expression of deo-operon escherichia coli k-12 genes in the makeup of hybrid plasmid rp4::mu-deo in pseudomonas trifolii and pseudomonas putida]. 1979113191
pathways of d-fructose catabolism in species of pseudomonas.cell-free extracts of d-fructose grown cells of pseudomonas putida, p. fluorescens, p. aeruginosa, p. stutzeri, p. mendocina, p. acidovorans and p. maltophila catalyzed a p-enolpyruvate-dependent phosphorylation of d-fructose and contained 1-p-fructokinase activity suggesting that in these species fructose-1-p and fructose-1,6-p2 were intermediates of d-fructose catabolism. neither the 1-p-fructokinase nor the activity catalyzing a p-enolpyruvate-dependent phosphorylation of d-fructose was prese ...1977139135
deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.plasmid and phage deoxyribonucleic acid (dna) harboring bacterial insertion sequence (is) elements is1, is2, and is5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. the hybridization method used permits the detection of sequences partially homologous to the elements. hybridization of the is-containing probes to each other revealed a region of limited homology between is1 and is2. homologous sequences were then detected by comput ...1979159291
mandelate racemase from pseudomonas putida. magnetic resonance and kinetic studies of the mechanism of catalysis.the interactions of mandelate racemase with divalent metal ion, substrate, and competitive inhibitors were investigated. the enzyme was found by electron paramagnetic resonance (epr) to bind 0.9 mn2+ ion per subunit with a dissociation constant of 8 mum, in agreement with its kinetically determined activator constant. also, six additional mn2+ ions were found to bind to the enzyme, much more weakly, with a dissociation constant of 1.5 mm. binding to the enzyme at the tight site enhances the effe ...1975164210
magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b-5 and cytochrome p-450-cam.magnetic circular dichroism spectra are reported for the visible and near ultraviolet spectral regions of liver microsomes from dimethylbenzanthracene-treated rats. the sequential addition of nadh, dithionite, and carbon monoxide enables us to determine contributions to the magnetic circular dichroism by cytochromes b-5 and p-450, which dominate the spectra. the magnetic circular dichroism of the microsomal preparation is compared with that of purified oxidized and reduced cytochrome -b-5 from p ...1975164936
physiological role for the membrane bound ascorbate-tmpd oxidase in pseudomonas putida.the activity of the membrane-bound ascorbate-tmpd oxidase in pseudomonas putida varies with growth conditions and age of the culture. a comparison of the effects of cyanide and azide on the oxidation of various substrates suggests that ascorbate-tmpd oxidase is not the terminal oxidase for nadh or succinate oxidation. however, it does have a role in the oxidation of nicotinate, and may act as an additional terminal oxidase under certain other growth conditions.1975168828
regulation of arginine and pyrimidine biosynthesis in pseudomonas putida.repression of biosynthetic enzyme synthesis in pseudomonas putida is incomplete even when the bacteria are growing in a nutritionally complex environment. the synthesis of four of the enzymes of the arginine biosynthetic pathway (n-acetyl-alpha-glutamokinase/n-acetylglutamate-gamma-semialdehyde dehydrogenase, ornithine carbamoyltransferase and acetylornithine-delta-transaminase) could be repressed and derepressed, but the maximum difference observed between repressed and derepressed levels for a ...1976176312
plasmid-determined alcohol dehydrogenase activity in alkane-utilizing strains of pseudomonas putida.we have identified an alcohol dehydrogenase activity in pseudomonas putida strains carrying the cam-oct degradative plasmid that were grown on octane. the activity is nicotinamide adenine dinucleotide independent, sediments at 48,000 x g, and shows 20-fold greater activity with octanol rather than butanol as substrate. the enzyme is inducible by unoxidized alkane and is present only in strains that have the oct plasmid genes for alkane degradation with a wild-type alco locus. no analogous chromo ...1976177405
kinetic studies on a 4-methoxybenzoate o-demethylase from pseudomonas putida.a direct, sensitive and reliable photometric assay procedure for monitoring the activity of non-specific 4-methoxybenzoate o-demethylases of microorganisms is described. the assay is based on the o-demethylation of 3-nitro-4-methoxybenzoate to the yellow-coloured product 3-nitro-4-hydroxybenzoate. using this assay and by monitoring the oxidation rate of reduced pyridine nucleotides, the kinetic properties of a purified, reconstituted enzyme system composed of 4-methoxybenzoate monooxygenase (o-d ...1977188654
amino acid sequence of a peptide containing an essential cysteine residue of escherichia coli gmp synthetase.the amino acid sequence of a 51-residue tryptic peptide of citraconylated [1-14c]carboxamidomethyl-labeled escherichia coli gmp synthetase was determined by sequenator analyses of the intact peptide and fragments obtained by cleavage of the peptide with cyanogen bromide, trypsin, and staphylcoccus aureus strain v8 protease. the cysteine residue of this peptide fragment is essential for glutamine-dependent gmp synthesis activity and is implicated in formation of a hypothetical covalent glutamyl-e ...1978213434
characterization of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1.nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, was purified to homogeneity, as judged by sodium dodecyl sulfate disc gel electrophoresis and ultracentrifugation, from benzoate-induced cells of pseudomonas arvilla. the molecular weight of the enzyme was determined to be 38,300 by sedimentation equilibrium analysis, 37,000 by sephadex g-100 gel filtration, and 37,500 by sodium dodecyl sulfate disc gel electrophoresis, respectively, indicating that the enzyme consisted ...1978214433
[regulation and properties of a particular acceptor-dependent alcohol dehydrogenase of pseudomonas putida during growth on n-alkanes]. 1978216166
comparison of microsomal and solubilized monooxygenases from rat and rabbit by proton magnetic relaxation.the paper presents results of a comparative study of the haem environment, by proton magnetic relaxation, in p-450 and p-448 monooxygenases from rat and rabbit, induced by phenobarbital and 3-methylcholanthrene, in both species. it was established that the method yields information on the accessibility of the haem iron for solvent molecules (protons), both in microsomes and in solubilized samples of various degrees of purification, i.e. association. the state of micelles in the solutions does no ...1979229678
a cleavage map of the tol plasmid of pseudomonas putida mt-2.a cleavage map of the tol plasmid pwwo has been determined for the restriction endonucleases hindiii and xhoi. a number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified xhoi fragments to southern blots of hindiii digest products and (iii) analysis of a number of deletion mutants.1979231727
chromosomal mobilization from a reca mutant of pseudomonas putida.a methyl methane sulfonate sensitive mutant of p. putida strain ppg1 is also extremely sensitive to uv-rays, compared to parent wild type cells. this mutant behaves typically as recombination less (reca) mutants of escherichia coli and pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. sex factor plasmids such as k-xyl or tol can mobilize chromosomal genes equally well bot ...1979232230
initial reactions in the oxidation of naphthalene by pseudomonas putida.a strain of pseudomonas putida that can utilize naphthalene as its sole source of carbon and energy was isolated from soil. a mutant strain of this organism, p. putida 119, when grown on glucose in the presence of naphthalene, accumulates optically pure (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene in the culture medium. the cis relative stereochemistry in this molecule was established by nuclear magnetic resonance spectrometry. radiochemical trapping experiments established that this cis d ...1975234247
catalytic and thermodynamic properties of the urocanate hydratase reaction.urocanate hydratase (4-imidazolone-5-propionate hydro-lyase, ec isolated from pseudomonas putida contains covalently bound alpha-ketobutyrate as its cofactor. in the process of examining the mechanism by which alpha-ketobutyrate serves in this capacity, various thermodynamic parameters and temperature effects on urocanate hydratase activity were determined. as the equilibrium constant at 15 degrees c for imidazooone propionate formation from urocanate is approximately 69, regardless of ...1975235308
metabolism of resorcinylic compounds by bacteria. purification and properties of acetylpyruvate hydrolase from pseudomonas putida 01.acetylpyruvate hydrolase, the terminal inducible enzyme of the pathway of orcinol catabolism in pseudomonas putida, catalyzes the quantitative conversion of acetylpyruvate into acetate and pyruvate. the enzyme has been purified approximately 40-fold from extracts of ps. putida grown on orcinol. disc gel electrophoresis of the preparations show one major and one minor band of protein. the molecular weight of the enzyme is approximately 38,000 by sodium dodecyl sulfate electrophoresis. acetylpyruv ...1975236305
aromatic aldehyde dehydrogenase from pseudomonas putida n.c.i.b. 9869. 1975236959
2-keto-3-deoxy-6-phosphogluconic aldolase from pseudomonas putida. 1975237184
allantoin racemase: a new enzyme from pseudomonas species.1. allantoin racemase is a novel enzyme which catalyzes the conversion of s(+)-and r(minus)-allantoin into the racemate. 2. the enzyme is present in pseudomonas testosteroni, pseudomonas putida and five biotypes of pseudomonas fluorescens, but absent in a number of other pseudomonas species. 3. the enzyme of ps. testosteroni was purified 133-fold and exposes optimal activity at ph 8.0-8.2 and 50 degrees c. the enzyme is stable on heating for 15 min at 70 degrees c. 4. the enzyme appeared to be s ...1975237557
crystallization and properties of l-arginine deiminase of pseudomonas putida.crystalline l-arginine deiminase of pseudomonas putida was prepared by the following steps: sonic disruption, ammonium sulfate fractionation, protamine sulfate treatment, deae-cellulose column chromatography, and l-arginine-sepharose 6b chromatography followed by crystallization. this procedure yields a crystalline pure enzyme with a 45% recovery of the activity in crude cell-free extracts. the yield is significantly higher than that reported for this enzyme. the purified enzyme appears to be ho ...1975237904
intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the l-arginine catabolic pathway of pseudomonas pseudomonas putida p2 grown on l-arginine as the sole source of carbon and nitrogen, catabolism of l-arginine forms of alpha-ketoarginine, gamma-guanidinobutyrate, and gamma-aminobutyrate. a previously undetected intermediate, gamma-guanidinobutyraldehyde, is identified as the product of alpha-ketoarginine decarboxylase. an 86-fold purification of this enzyme is described. activity is thiamine pyrophosphate-dependent and cofactor reassociation is facilitated by divalent cations. the order of ...1975237915
extradiol cleavage of 3-substituted catechols by an intradiol dioxygenase, pyrocatechase, from a pseudomonad.pyrocatechase (catechol 1,2-oxidoreductase (decyclizing), ec, a ferric ion-containing dioxygenase from pseudomonas arvilla c-1, catalyzes the intradiol cleavage of catechol with insertion of 2 atoms of molecular oxygen to form cis,cis-muconic acid. the enzyme also catalyzed the oxidation of various catechol derivatives, including 4-methylcatechol, 4-chlorocatechol, 4-formylcatechol (protocatechualdehyde), 4,5-dichlorocatechol, 3,5-dichlorocatechol, 3-methylcatechol, 3-methoxycatechol, ...1975238971
a 4-methoxybenzoate o-demethylase from pseudomonas putida. a new type of monooxygenase system.a strain of pseudomonas putida grown on 4-methoxybenzoate as sole carbon source contains an enzyme system for the o-demethylation of this substrate. the enzyme system is purifiable and can be separated into two components: an nadh-dependent reductase and an iron-containing and acid-labile-sulfur-containing monooxygenase. the reductase, of molecular weight 42000 and containing two chromophores, an fmn and an iron-sulfur complex (epr at g = 1.95), reduces both one-electron and two-electron accepto ...1975240720
an endonuclease cleavage map of the plasmid pwwo-8, a derivative of the tol plasmid of pseudomonas putida mt-2.cleavage sites on the pwwo-8 plasmid were determined for the restriction endonucleases hindiii and xhoi. terminal labelling using dna polymerase i was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid.1979285316
origins of metabolic diversity: evolutionary divergence by sequence repetition.recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ...1979291059
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...1977329101
[pseudomonas putida: identification, antibiotic sensitivity and pathogenicity (author's transl)].this work studies 51 strains of pseudomonas putida, isolated from clinical specimens (17) and hospital environment (34). identification is performed by study of 41 physiologica and biochemical characters and 78 nutritional characters. according to the two biotypes a and b, described by stanier, palleroni and doudoroff, these 51 strains can be grouped as follows: 48 have typical characters of biotype a, widely predominant, 3 can be distinguished from biotype a only by their auxanogram and include ...1977341053
lipoate metabolism in pseudomonas putida lp: thiolsulfinates of lipoate and bisnorlipoate. 1978343716
bacterial catabolism of lipoic acid. isolation and identification of a methyl ketone.a soil bacterium, pseudomonas putida lp, can be grown on lipoate as sole source of carbon, sulfur, and energy. in addition to the previously identified catabolites (bisnorlipoate, tetranorlipoate, beta-hydroxybisnorlipoate, lipoate thiolsulfinate, and two bisnorlipoate thiolsulfinates) isolated from cultures of the organism grown on [1,6-14c[lipoate, a methyl ketone (1,2-dithiolane-3-butyl-3'-one) has now been isolated and identified. this catabolite was isolated by solvent extraction and hydrop ...1978357321
anabolic ornithine carbamoyltransferase of escherichia coli and catabolic ornithine carbamoyltransferase of pseudomonas putida. steady-state kinetic analysis.the anabolic and catabolic ornithine carbamoyltransferases of pseudomonas putida display an undirectional catalytic specialization: in citrulline synthesis for the anabolic enzyme, in citrulline phosphorolysis for the catabolic one. the irreversibility of the anabolic enzyme in vitro has been previously explained by its kinetic properties, whereas the irreversibility of the catabolic transferase in vivo was shown to be due to its allosteric behaviour. in this work a steady-state kinetic analysis ...1978359326
microbiological transformations of terpenes: part xxv--enzymes involved in the degradation of linalool in the pseudomonas incognita, linalool strain. 1978367951
expression of escherichia coli tryptophan operon in rhizobium leguminosarum.rp4-trp hybrid plasmid containing escherichia coli whole tryptophan operon was conjugatively transferred from e. coli to rhizobium leguminosarum strains carrying mutations in different trp genes, converting their trp- phenotype to trp+. that the phenotype change of the r. leguminosarum cells was due to the presence of the e. coli tryptophan operon was verified by the isolation of rp4-trp hybrid plasmid from the r. leguminosarum conjugant cells, and by re-transfer of rp4-trp plasmid by conjugatio ...1979375025
nonfermentative bacilli: evaluation of three systems for identification.three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ...1979389945
mercury and organomercurial resistances determined by plasmids in pseudomonas.mercury and organomercurial resistance determined by genes on ten pseudomonas aeruginosa plasmids and one pseudomonas putida plasmid have been studied with regard to the range of substrates and the range of inducers. the plasmidless strains were sensitive to growth inhibition by hg(2+) and did not volatilize hg(0) from hg(2+). a strain with plasmid rp1 (which does not confer resistance to hg(2+)) similarly did not volatilize mercury. all 10 plasmids determine mercury resistance by way of an indu ...1977410779
fractionation of inducible alkane hydroxylase activity in pseudomonas putida and characterization of hydroxylase-negative plasmid mutations.the plasmid-determined inducible alkane hydroxylase of pseudomonas putida resolved into particulate and soluble fractions. spinach reductase and spinach ferredoxin could replace the soluble hydroxylase component. two alkane hydroxylase mutants show in vitro complementation (s. benson and j. shapiro, j. bacteriol., 123: 759-760, 1975): one, alk-7, lacks an active soluble component and the other, alk-181, lacks an active particulate component. together with previous results on a particulate alcoho ...1977410794
regulatory mutations of the pseudomonas plasmid alk regulon.pseudomonas putida strains with plasmids carrying pleiotropic alk mutations gave rise to alkane-positive "revertants," which differ from wild type. some had restricted ability to utilize alkane and primary alcohol growth substrates, and others could grow on undecane and dodecanol, which are not utilized by alk+ strains. these revertants showed altered responses to normal inducers of alka+, alkb+, and alkc+ activities. some revertants were constitutive for these activities. constitutive mutants c ...1977410795
tol plasmid in pseudomonas aeruginosa pao: thermosensitivity of self-maintenance and inhibition of host cell growth.the tol plasmid originally isolated in pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of pseudomonas, i.e., p. putida, p. fluorescens, and p. aeruginosa, except for a strain of p. aeruginosa, strain pao. the same strain, however, could accept the plasmid when its restriction and modification abilities were lost by mutations or by growing at high temperature. in addition, the transmissibility of the tol plasmid from strain pao to p. putida was low when the ...1978415040
rsf1010 plasmid as a potentially useful vector in pseudomonas species.rsf1010 plasmid dna was introduced into pseudomonas putida and p. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in pseudomonas species. the number of copies of rsf1010 was 43 per chromosome equivalent in p. putida cells.1978417070
isolation of tol and rp4 recombinants by integrative suppression.we obtained genetic and molecular evidence of non-thermosensitive recombinants of rp4 (kmr tcr cbr/apr) and the thermosensitive tol plasmid. as first isolated in pseudomonas aeruginosa pao, the recombinant plasmid ptn1 specified noninducible synthesis of tol enzymes and was transmissible to escherichia coli on selection for the transfer of kanamycin resistance. the phenotypic expression of tol genes of ptn1 in e. coli was low and also noninducible. a spontaneous segregant, ptn2, appearing from p ...1978418059
alpha-hydroxyglutarate as an intermediate in the catabolism of alpha-aminoadipate by pseudomonas putida. 1979429347
properties of the iron--sulphur proteins of the benzene dioxygenase system from pseudomonas putida.a purification procedure was developed to stabilize the iron-sulphur proteins of the benzene dioxygenase system from pseudomonas putida. the intermediate electron-carrying protein has a mol. wt. of 12300 and possesses one (2fe--2s) cluster, whereas the terminal dioxygenase has a mol.wt. of 215300 and possesses two (2fe--2s) clusters. the order and stoicheiometry of electron transfer and of the whole system are described.1979435241
interaction of the chiral pyruvate analog, 2-keto-3-bromobutyrate, with pyruvate lyases. 2-keto-3-deoxygluconate-6-phosphate aldolase of pseudomonas putida.the enzyme 2-keto-3-deoxygluconate-6-p aldolase of pseudomonas putida is inactivated by one of the chiral forms of 2-keto-(3rs)-3-bromobutyric acid (bromoketobutyrate). the inactivation shows saturation kinetics and competition with pyruvate. the minimal inactivation half-time is 4 min and that concentration of bromoketobutyrate half-saturating the enzyme is 2 mm. (3rs)-[3-3h]bromoketobutyrate is catalytically detritiated during enzyme inactivation. a kinetic analysis of rates gave data consiste ...1979447683
raman spectrum of protocatechuate dioxygenase from pseudomonas putida. new low frequency bands. 1979454431
structural comparison of gamma-carboxymuconolactone decarboxylase and muconolactone isomerase from pseudomonas putida. 1979454663
a theoretical interpretation of the variations of some physical parameters within the [2fe-2s] ferredoxin group.a model is proposed to explain the variation of some physical parameters within the reduced [2fe-2s] ferredoxin group. according to this model, the main effects result from a variable mixing of some d orbitals of the fe2+ ion owing to rhombic distortion of the active site having the same geometrical character, but different in intensity, for each protein. some peculiar experimental results such as the axial electron paramagnetic resonance spectra of adrenal ferredoxin and pseudomonas putida ferr ...1979465523
nonidentical subunits of pyrocatechase from pseudomonas arvilla c-1. 1979475379
transport of c5 dicarboxylate compounds by pseudomonas putida.induced glutarate and 2-oxoglutarate uptake and transport by pseudomonas putida were investigated in whole cells and membrane vesicles, respectively. uptake of 2-oxoglutarate, but not glutarate, was against a concentration gradient to 1.7-fold greater than the initial extracellular concentration. membrane vesicles transported 2-oxoglutarate and glutarate against gradients to intramembrane concentrations fivefold greater than the initial extravesicle concentrations. the rates of transport of both ...1979479107
autoradiographic study of the localization and evolution of growth zones in bacterial colonies.incorporation of [3h]leucine in the bacteria of 18 to 48 h-old colonies of pseudomonas aeruginosa, pseudomonas putida, bacillus thuringiensis, staphylococcus aureus and escherichia coli enabled the localization of bacterial multiplication sites by means of autoradiography of sagittal sections. in colonies where fast diameter expansion occurred, all the bacteria from the peripheral corona contributed to peripheral growth; in colonies where the expansion was slower, the growth rate of the bacteria ...1979479831
program of bacteriophage gh-1 dna transcription in infected pseudomonas putida.the program of transcription in phage gh-1-infected pseudomonas putida was examined. it was found that the host p. putida rna polymerase transcribes early rna from the l strand of gh-1 dna during the initial stages of infection. the host rna polymerase is also undoubtedly responsible for transcription of complementary rna late in the infectious cycle because complementary rna was not transcribed when rifampin was added to the infected cell culture. the gh-1-induced rna polymerase transcribes lat ...1979480467
[dechlorination of 4-chlorophenol following extradiolic ring cleavage by pseudomonas putida]. 1979483865
the amino acid composition of histidine ammonialyase from pseudomonas putida ncib 10807.the amino acid composition of histidine ammonia-lyase from pseudomonas putida ncib 10807 suggests that this enzyme may be different from the pseudomonas testosteroni ncib 10808 histidine ammonia-lyase, whose amino acid composition is known.1979488259
creatinine amidohydrolase (creatininase) from pseudomonas putida. purification and some properties. 1979500580
regulatory properties of histidase from pseudomonas putida. 1979518595
electron microscopy of some rock phosphate dissolving bacteria and fungi.bacteria pseudomonas striata, bacillus polymyxa, b. megaterium and b. pulvifaciens, and fungi aspergillus awamori, a. niger and penicillium digitatum dissolve tricalcium phosphate and, much less, mussorie and udaipur rock phosphate. the solubilizing power of fungi was higher than that of bacteria, the highest being with a. awamori and a. niger, and with p. striata. electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. the siz ...1979527907
local anesthetics block induction of the pseudomonas alk regulon.the local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in pseudomonas putida. procaine reversibly inhibited existing alkane hydroxylase activity. induction of a soluble aliphatic amidase activity was not affected. these results support the hypothesis that induction of the plasmid-determined alkane metabolic system in p. putida involves a membrane component(s).1979533765
regulation of membrane peptides by the pseudomonas plasmid alk regulon.pseudomonas putida strains carrying the plasmid alk genes will grow on n-alkanes. induced alk+ strains contain membrane activities for alkane hydroxylation and dehydrogenation of aliphatic primary alcohols. p. putida cytoplasmic and outer membranes can be separated by sucrose gradient centrifugation after disruption of cells by either mild detergent lysis or passage through a french press. both the membrane component of alkane hydroxylase and membrane alcohol dehydrogenase fractionated with the ...1979533768
coexistence of different pathways in the metabolism of n-propylbenzene by pseudomonas sp.pseudomonas desmolytica s449b1 and pseudomonas convexa s107b1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. the same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. a ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. together with ...1979543699
[purification and crystallization of a superoxide dismutase from pseudomonas putida]. 1979551636
cometabolism of products of 1,1,1-trichloro-2,2-bis (p-chlorophenyl)ethane (ddt) by pseudomonas putida. 1977560402
the metabolism of caffeine by a pseudomonas putida strain.1) a bacterium capable of growing aerobically with caffeine (1,3,7-trimethylxanthine) as sole source of carbon and nitrogen was isolated from soil. the morphological and physiological characteristics of the bacterium were examined. the organism was identified as a strain of pseudomonas putida and is referred to as pseudomonas putida c1. 15 additional caffeine-degrading bacteria were isolated, and all of them were also identified as pseudomonas putida strains. the properties of the isolates are d ...1977561017
[interrelationships between carnitine metabolism and fatty acid assimilation in pseudomonas putida (author's transl)].the carnitine metabolism and some relations to the fatty acid metabolism were studied in pseudomonas putida by means of control of growth, analysis of metabolites, and determination of enzyme activites. the strain grew on gamma-butyrobetaine, d,l- and l-carnitine, glycinebetaine, choline, d,l-norcarnitine, d,l-gamma-amino-beta-hydroxybutyrate, and d,l-beta-hydroxybuty-rate. although the strain used straight-chain fatty acids of 2-16 c-atoms, it was only able to grow on o-acyl-l-carnitines of 10 ...1978565193
formaldehyde dehydrogenase from pseudomonas putida. purification and some properties.formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of pseudomonas putida c-83 by chromatographies on columns of deae-cellulose, deae-sephadex a-50, and hydroxyapatite. the purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at ph 7.8 using formaldehyde as a substrate. the enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. the enzyme w ...1979571868
stereochemistry of the conversion of methacrylate to beta-hydroxyisobutyrate in pseudomonas putida. 1979572832
stereospecific hydrogen loss in the conversion of [2h7]isobutyrate to beta-hydroxyisobutyrate in pseudomonas putida. the stereochemistry of beta-hydroxyisobutyrate dehydrogenase. 1979573276
transposition of a beta-lactamase locus from rp1 into pseudomonas putida degradative plasmids.the beta-lactamase gene from the rp1 plasmid transposes into at least two pseudomonas putida degradative plasmids. donor strains that carry rp1 (bla+ tet+ apha+) and a degradative plasmid yield transconjugants that have only the bla+ marker of rp1. this occurs in up to 80% of all bla+ transconjugants. segregation of the bla+ marker requires the presence of a degradative plasmid in the donor and is only observed in transconjugants that have received degradative markers. the bla+ tet apha transcon ...1977584205
the purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from pseudomonas putida.the enzyme that catalyses the hydroxylation of the methyl group of p-cresol was purified from pseudomonas putida. it has mol.wt. 115000 and appears to contain two subunits of equal molecular weight. one subunit is a c-type cytochrome and the other is a flavoprotein. reduction of the cytochrome occurred on addition of substrate. the same enzyme catalyses both p-cresol hydroxylation and the further oxidation of the product, 4-hydroxybenzyl alcohol. the stoicheiometry of acceptor reduced per molecu ...1977588247
metabolism of dibenzothiophene by a beijerinckia species.beijerinckia b8/36 when grown with succinate in the presence of dibenzothiophene, accumulated (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene and dibenzothiophene-5-oxide in the culture medium. each metabolite was isolated in crystalline form and characterized by a variety of chemical techniques, cis-naphthalene dihydrodiol dehydrogenase, isolated from pseudomonas putida, oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene to a compound that was tentatively identified as 1,2-dihydroxydi ...1977596875
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
alpha-pinene metabolism by pseudomonas using metabolically altered mutants and acrylate, novel putative intermediates of alpha-pinene metabolism by pseudomonas putida pin11 were detected. they were characterized as 3-isopropylbut-3-enoic acid and (zeta)-2-methyl-5-isopropylhexa-2,5-dienoic acid.1977597274
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