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intermediate products in tartrate decomposition by cell-free extracts of pseudomonas putida under anaerobic conditions. 195613363889
specificity of whole cells and cell-free extracts of pseudomonas putida towards (+), (-), and meso-tartrate. 195613373875
the pathway of creatine catabolism by pseudomonas ovalis. 195613319640
effect of growth substrates on isocitratase formation by pseudomonas ovalis chester. 195813600353
[differentiation of pseudomonas aeruginosa compared with pseudomonas fluorescens & pseudomonas putida on ttc culture medium]. 195813606774
salicylate as intermediate in the breakdown of aromatic ring by pseudomonas convexa var. hippuricum. 195913629391
formation of malate from glycollate by pseudomonas ovalis chester. 195914411058
enzymic formation of citramalate from acetyl-coenzyme a and pyruvate in pseudomonas ovalis chester, catalysed by "pyruvate transacetase". 196013708034
[importance of the oxidase reaction in the identification of pseudomonas aeruginosa, pseudomonas fluorescens and pseudomonas putida]. 196014409895
taxonomic relationships among the pseudomonads.colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ...196113694873
crystalline tartronic semialdehyde reductase from pseudomonas ovalis chester. 196113707448
[assimilation of (--)-carnitine by pseudomonas ovalis]. 196314059240
oxaloacetate 4-carboxy-lyase from pseudomonas ovalis chester. 196414205502
metabolism of p-hydroxyphenylacetic acid in pseudomonas ovalis. 196414263147
microbial degradation of isopropyl-n-3 -chlorophenylcarbamate and 2-chloroethyl-n-3-chlorophenylcarbamate.microbial degradation of isopropyl-n-3-chlorophenylcarbamate (cipc) and 2-chloroethyl-n-3-chlorophenylcarbamate (cepc) was observed in a soil perfusion system. degradation in perfused soils, and by pure cultures of effective bacterial isolates, was demonstrated by the production of 3-chloroaniline and the subsequent liberation of free chloride ion. identified isolates effective in degrading and utilizing cipc as a sole source of carbon included pseudomonas striata chester, a flavobacterium sp., ...196514325285
crystallization and some properties of 3,4-dihydroxyphenylacetate 2,3-oxygenase from pseudomonas ovalis. 19655857427
[investigations on the oxidative nicotine reduction by pseudomonas convexa(strain 22c)]. 19655899300
the conversion of catechol and protocatechuate to beta-ketoadipate by pseudomonas putida. 19665916391
the conversion of catechol and protocatechuate to beta-ketoadipate by pseudomonas putida. ii. enzymes of the protocatechuate pathway. 19665916392
the conversion of catechol and protocatechuate to beta-ketoadipate by pseudomonas putida. iv. regulation. 19665916393
density heterogeneity in pseudomonas putida bacteriophage preparations. 19665926748
synthesis of the enzymes of the mandelate pathway by pseudomonas putida. i. synthesis of enzymes by the wild type.hegeman, g. d. (university of california, berkeley). synthesis of the enzymes of the mandelate pathway by pseudomonas putida. i. synthesis of enzymes by the wild type. j. bacteriol. 91:1140-1154. 1966.-the control of synthesis of the five enzymes responsible for the conversion of d(-)-mandelate to benzoate by pseudomonas putida was investigated. the first three compounds occurring in the pathway, d(-)-mandelate, l(+)-mandelate, and benzoylformate, are equipotent inducers of all five enzymes. a n ...19665929747
synthesis of the enzymes of the mandelate pathway by pseudomonas putida. ii. isolation and properties of blocked mutants.hegeman, g. d. (university of california, berkeley). synthesis of the enzymes of the mandelate pathway by pseudomonas putida. ii. isolation and properties of blocked mutants. j. bacteriol. 91:1155-1160. 1966.-mutants of pseudomonas putida blocked in early reactions of the pathway for oxidation of d-mandelate were isolated and partially characterized. the specific genetic lesions in these mutants made normal inducer-metabolites of the pathway nonmetabolizable. under the conditions of gratuitous e ...19665929748
synthesis of the enzymes of the mandelate pathway by pseudomonas putida. 3. isolation and properties of constitutive mutants.hegeman, g. d. (university of california, berkeley). synthesis of the enzymes of the mandelate pathway by pseudomonas putida. iii. isolation and properties of constitutive mutants. j. bacteriol. 91:1161-1167. 1966.-mutants of pseudomonas putida constitutive for the synthesis of l(+)-mandelate dehydrogenase were obtained after mandelate- or benzoylformate-limited growth in a chemostat. when grown in media noninducing for the wild type, the mutants are capable of coordinate, constitutive synthesis ...19665929749
characterization of bacteriophage gh-1 for pseudomonas putida.lee, lucy f. (michigan state university, east lansing), and j. a. boezi. characterization of bacteriophage gh-1 for pseudomonas putida. j. bacteriol. 92:1821-1827. 1966.-bacteriophage gh-1 of pseudomonas putida a.3.12 was isolated and purified by differential centrifugation and diethylaminoethyl (deae) cellulose chromatography. an electron micrograph of the phage stained with uranyl acetate revealed a regular hexagonal outline about 50 mmu across with a short wedge-shaped tail attached at one co ...19665958111
cofactor requirements of the l-malate dehydrogenase of pseudomonas ovalis chester.1. the l-malate dehydrogenase of pseudomonas ovalis chester, which is independent of nicotinamide nucleotides and which is structurally and functionally bound to the cell-wall membrane, has been prepared in a soluble form and partially purified. 2. the purified dehydrogenase exhibits a triple cofactor requirement for fad, quinone and phospholipid, and in the presence of these cofactors can utilize 2,6-dichlorophenol-indophenol as hydrogen acceptor. 3. the formation of reduced forms of fad was no ...19665966284
inducibility of tryptophan synthetase in pseudomonas putida. 19665229989
crystallization and properties of p-hydroxybenzoate hydroxylase from pseudomonas putida. 19664380381
the conversion of catechol and protocatechuate to beta-ketoadipate by pseudomonas putida. 3. enzymes of the catechol pathway. 19665330966
metabolism of pipecolic acid in a pseudomonas species iv. electron transport particle of pseudomonas putida.baginsky, marietta l. (university of california, san francisco medical center, san francisco), and victor w. rodwell. metabolism of pipecolic acid in a pseudomonas species. iv. electron transport particle of pseudomonas putida. j. bacteriol. 92:424-432. 1966.-enzymes of pseudomonas putida p2 catalyzing oxidation of pipecolate to delta(1)-piperideine-6-carboxylate are located in a subcellular fraction sedimenting at 105,000 x g. since this fraction resembles the mammalian electron transport parti ...196616562131
a phage-initiated polysaccharide depolymerase in pseudomonas putida. 19676028945
two new metabolites, 2- nonaprenylphenol and 2-nonaprenyl-3-methyl-6-methoxy-1, 4-benzoquinone, from pseudomonas ovalis. 19676040283
computer simulation of fermentation systems.results of batch fermentation of gluconic acid by pseudomonas ovalis were graphically analyzed to obtain a kinetic model to represent the data. since gluconic acid was produced by the hydrolysis of a lactone intermediate, the model was necessarily represented by a set of kinetic equations. a computer simulation technique involving the use of the midas program was developed to solve the system of nonlinear equations and to check the appropriateness of the model. since the maximal specific growth ...19676049291
metabolism of pipecolic acid in a pseudomonas species. v. pipecolate oxidase and dehydrogenase.oxidation of pipecolate to delta(1)-piperideine-6-carboxylate is catalyzed by pipecolate oxidase, an inducible, membrane-bound dehydrogenase associated with the electron transport components of pseudomonas putida p2. from the oxidase, we obtained a smaller particle containing flavine adenine dinucleotide (fad) and cytochrome b, but no longer able to catalyze electron transfer to oxygen or to cytochrome c. certain properties of this l-pipecolate dehydrogenase, an fad-flavoprotein, are reported.19676051341
cold-sensitive mutation of pseudomonas putida affecting enzyme synthesis at low temperature.a cold-sensitive mutant of pseudomonas putida has been isolated which grows normally at 30 c but is unable to grow on mandelate as a source of carbon at 15 c. the mutation results in the inability of the strain to carry out the reaction catalyzed by cis,cis-muconate lactonizing enzyme at low temperature and must lie in the structural gene for that enzyme, because the mutant enzyme produced at 30 c shows altered thermal stability. the mutant enzyme is not intrinsically cold-labile, nor is it cold ...19676074402
sedimentation analysis of pseudomonas putida a.3.12 bacteriophage gh-1 deoxyribonucleic acid. 19675621492
enzymes of the tryptophan synthetic pathway in pseudomonas putida.the first four enzymatic activities of the tryptophan synthetic pathway in pseudomonas putida were found on separate molecules. gel filtration and density gradient centrifugation experiments did not disclose any associations or aggregations among them. these findings contrast with the situation found in the enteric bacteria, where the first two activities are found in an aggregate and the third and fourth are catalyzed by a single enzyme. tryptophan synthetase, the last enzyme of the pathway, co ...19685636809
transducing phage for pseudomonas putida. 19685641761
regulation of histidine catabolism by succinate in pseudomonas putida.the regulation of the histidine-degrading pathway is known to involve induction and repression. our studies have shown that succinate may control the histidine-degrading pathway by sequential negative feedback inhibition. succinate inhibited urocanase, and urocanate in turn inhibited histidase. crude preparations of the two enzymes were made from pseudomonas putida grown on l-histidine. succinate was a competitive inhibitor of urocanase (k(i), 1.8 mm). lactate, pyruvate, alpha-ketoglutarate, and ...19685674054
metabolism of beta-methylaspartate by a pseudomonad.a bacterium was isolated from soil which utilizes threo-beta-methyl-l-aspartate, certain other amino acids, and a variety of organic substances as single energy sources. it is, or closely resembles, pseudomonas putida biotype b. the ability of this organism to rapidly decompose such amino acids is dependent on inducible enzyme systems. dialyzed cell-free extracts of this bacterium metabolize beta-methylaspartate only when catalytic amounts of alpha-ketoglutarate, or pyruvate, and pyridoxal phosp ...19685724974
fine structure mapping of the tryptophan genes in pseudomonas putida. 19685731751
[degradation of deoxysurgars by bacterial enzymes. v. purification and characterization of an nadp-dependent abequose dehydrogenase from pseudomonas putida]. 19684387016
[on the decomposition of deoxy sugars by vacterial enzymes, iv. comparative studies on the oxidation of 3-deoxy-d-galactose and d-galactose in a strain of pseudomonas putida]. 19684387175
the metabolism of thymol by a pseudomonas.1. pseudomonas putida when grown with thymol contained a meta-fission dioxygenase, which required ferrous ions and readily cleaved the benzene nucleus of catechols between adjacent carbon atoms bearing hydroxyl and isopropyl groups. 2. 3-hydroxythymo-1,4-quinone was excreted towards the end of exponential growth and later was slowly metabolized. this compound was oxidized by partially purified extracts only when nadh was supplied; the substrate for the dioxygenase appeared to be 3-hydroxythymo-1 ...19684303067
studies on p-hydroxybenzoate hydroxylase from pseudomonas putida. 19694390689
alpha-hydroxyglutarate oxidoreductase of pseudomonas putida.oxidation of d-alpha-hydroxyglutarate to alpha-ketoglutarate is catalyzed by d-alpha-hydroxyglutarate oxidoreductase, an inducible membrane-bound enzyme of the electron transport particle [etp; a comminuted cytoplasmic membrane preparation with enzymic properties and chemical composition resembling beef heart mitochondrial etp (1)] of pseudomonas putida p2 (p2-etp). treatment of p2-etp with a nonionic detergent yields a preparation with the sedimentation characteristics of a soluble enzyme, but ...19695354943
phenol and benzoate metabolism by pseudomonas putida: regulation of tangential pathways.catechol occurs as an intermediate in the metabolism of both benzoate and phenol by strains of pseudomonas putida. during growth at the expense of benzoate, catechol is cleaved ortho (1,2-oxygenase) and metabolized via the beta-ketoadipate pathway; during growth at the expense of phenol or cresols, the catechol or substituted catechols formed are metabolized by a separate pathway following meta (2,3-oxygenase) cleavage of the aromatic ring of catechol. it is possible to explain the mutually excl ...19695354952
regulation of the meta cleavage pathway for benzoate oxidation by pseudomonas putida.catechol or 2-hydroxymuconic semialdehyde cannot participate as functional inducers of the meta pathway for benzoate metabolism in pseudomonas putida. induction of the first two enzymes of the pathway must be mediated by benzoate, or its analogues, as primary substrate.19695359614
the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria.1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ...19694886765
herellea (acinetobacter) and pseudomonas ovalis (p. putida) from frozen foods.seventeen strains of herellea vaginicola (acinetobacter antitratus) and 8 of pseudomonas ovalis (p. putida), isolated from 23 (6.3%) of 364 samples of frozen, foil-pack foods, were identified and characterized morphologically and biochemically. herellea was isolated from 17 foods (4.7%), p. ovalis from 6 (1.6%). no mima were found. the food samples included precooked frozen meats, precooked and uncooked frozen vegetables, and uncooked frozen desserts. the bacteria were detected in the food with ...19694886860
inducible degradation of hydroxyproline in pseudomonas putida: pathway regulation and hydroxyproline uptake.studies in pseudomonas putida of the inducible degradation of hydroxyproline to alpha-ketoglutarate have indicated that either of the two epimers, hydroxy-l-proline or allohydroxy-d-proline, acts as an inducer of all the pathway enzymes. in a mutant lacking the first enzyme of the sequence, hydroxyproline-2-epimerase, which interconverts these two hydroxyproline epimers, either epimer is still equally active as an inducer of the remaining three enzymes, suggesting that each epimer has intrinsic ...19695764334
conversion of l-hydroxyproline to glutamate by extracts of strains of pseudomonas convexa and pseudomonas fluorescens. 19695766672
autonomous replication of a defective transducing phage in pseudomonas putida. 19695784055
isolation of spontaneous mutant strains of pseudomonas putida. 19695796751
defective phage and chromosome mobilization in pseudomonas putida.the transfer of transducing phage dna in association with the mandelate genetic region of pseudomonas putida strain prs1 (termed pfdm) has been achieved by growing together mandelate-positive ppg2 cells harboring pfdm as an extrachromosomal element and mandelate-deleted ppg1 strains. this transfer is analogous to sexual conjugation in the enterobacteria. the transfer of pfdm elements is always associated with chromosome mobilization and some rare recombinants acquire genetic donor ability. we ha ...19695271748
regulation of the synthesis of glyceraldehyde-3-phosphate dehydrogenase in pseudomonas putida. 196911946970
crystallization of orcinol hydroxylase from pseudomonas putida. 197011945483
anthranilate synthase enzyme system and complementation in pseudomonas species.anthranilate synthase in pseudomonas putida is a two component enzyme system. the proteins, termed as i and as ii, have respective molecular weights of 65,000 and 18,000. five additional pseudomonas species, both tryptophan requiring and independent strains, were examined and all were shown to contain similar two component systems. anthranilate formation by "amide transfer," with l-glutamine as nitrogen donor, requires both proteins; "amination," utilizing ammonium ion, proceeds at ph 9 with onl ...19705274451
identification of alpha-ketobutyrate as the prosthetic group of urocanase from pseudomonas putida. 19705412708
biosynthesis of ubiquinone in non-photosynthetic gram-negative bacteria.1. the polyprenylphenol and quinone complements of the non-photosynthetic gram-negative bacteria, pseudomonas ovalis chester, proteus mirabilis and ;vibrio o1' (moraxella sp.), were investigated. 2. ps. ovalis chester and prot. mirabilis were shown to contain 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 5-demethoxyubiquinones, ubiquinones, an unidentified 1,4-benzoquinone [2-polyprenyl-1,4-benzoquinone (?)] and ;epoxyubiquinones'. ;vibrio o1' was ...19705420949
photoactivation of urocanase in pseudomonas putida: possible role in photoregulation of histidine metabolism.irradiation by near-ultraviolet light of cells or extracts of pseudomonas putida increased the urocanase activity. irradiated cells exhibited enhanced catabolic activity on histidine and urocanate.19705429726
delta-aminovaleramidase of pseudomonas putida. 19705432799
incorporation of oxygen-18 into benzene by pseudomonas putida. 19705436152
properties of the inducible hydroxyproline transport system of pseudomonas putida.features of the transport system for hydroxyproline in a strain of pseudomonas putida were studied. a mutant, lacking hydroxyproline-2 epimerase and unable to metabolize hydroxy-l-proline, was shown to transport and accumulate this compound after induction. both entry and exit rates were examined, and kinetic constants for the reaction were determined. increasing the induction time from 0.5 to 3 hr increased the entry rate three- to fourfold but had only a small and variable effect on the exit r ...19705438054
the aspartate kinase of pseudomonas putida. regulation of synthesis and activity. 19705441401
homoserine dehydrogenase of pseudomonas putida. properties and regulation. 19705441405
the purification and characterization of adenosine triphosphate ribonucleic acid adenyltransferase from pseudomonas putida. 19705442825
mandelic acid racemase from pseudomonas putida. evidence favoring a carbanion intermediate in the mechanism of action. 19705458641
regulation of synthesis of early enzymes of p-hydroxybenzoate pathway in pseudomonas putida. 19705469168
oxidation of furan-2-carboxylate to 2-oxoglutarate by pseudomonas putida f2: studies of enzymology and electron transport. 19705472188
biosynthesis of amino acids from hydrocarbons. 3. determination of optimum conditions for pseudomonas arvilla. 19705515453
the genetic control of dissimilatory pathways in pseudomonas putida. 19705525301
purification and characterization of fumarase from pseudomonas putida. 19705529637
comparative immunological studies of two pseudomonas enzymes.crystalline preparations of muconate lactonizing enzyme and muconolactone isomerase, two inducible enzymes that catalyze successive steps in the catechol branch of the beta-ketoadipate pathway, were used to prepare antisera. both enzymes were isolated from a strain of pseudomonas putida biotype a. the antisera did not cross-react with enzymes of the same bacterial strain that catalyze the chemically analogous steps in the protocatechuate branch of the beta-ketoadipate pathway, carboxymuconate la ...19704986759
mandelic acid racemase from pseudomonas putida. purification and properties of the enzyme. 19704989844
formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by pseudomonas putida. 19704314232
spin-state changes in cytochrome p-450cam on binding of specific substrates.the electron paramagnetic resonance signals of the soluble p-450 cytochrome from pseudomonas putida were observed at temperatures from 4.2 to 80 degrees k. as isolated, p-450 has a signal typical of a low spin ferric-heme compound with sulfur as one of the axial ligands (g = 2.45, 2.26, 1.91(5)). we also detected a minor signal typical of high spin ferric heme (g = 8, 4, 1.8) equivalent to less than 7% of the heme at temperatures below 20 degrees k. on titration with the substrate, (+)-camphor, ...19704319883
pufification of a 4-methoxybenzoate o-demethylase from pseudomonas putida. 19714329101
methyrapone interaction with pseudomonas putida cytochrome p-405. 19714331031
the iron electron-nuclear double resonance (endor) of two-iron ferredoxins from spinach, parsley, pig adrenal cortex and pseudomonas putida. 19714331268
the coexistence of two metabolic pathways in the meta cleavage of catechol by pseudomonas putida n.c.i.b. 10105. 19714333847
the physiologic significance of the two divergent metabolic steps in the meta cleavage of catechols by pseudomonas putida n.c.i.b. 10105. 19714333848
mechanism of action of p-hydroxybenzoate hydroxylase from pseudomonas putida. 3. the enzyme-substrate complex. 19714398470
[fine structure of the wall of a pseudomonas putida]. 19714997330
aerobic metabolism of -amino-n-butyric acid by pseudomonas putida. 19714999027
properties and subunit structure of the b component of pseudomonas putida tryptophan synthetase. 19715000700
pathogenic significance of pseudomonas fluorescens and pseudomonas putida. 19715002396
[physiology of fluorescent pseudomonas and characteristics of pseudomonas putida]. 19715005193
metabolism of basic amino acids in pseudomonas putida. properties of the inducible lysine transport system. 19715547703
photoactivation of urocanase in pseudomonas putida: factors influencing activation. 19715549144
metabolism of basic amino acids in pseudomonas putida. catabolism of lysine by cyclic and acyclic intermediates. 19715554291
metabolism of basic amino acids in pseudomonas putida. intermediates in l-arginine catabolism. 19715570437
the breakdown of tropic acid in pseudomonas putida strain l. i. utilization of various substrates; the conversion of tropic acid into phenylacetic acid. 19715573355
new regulatory mutation affecting some of the tryptophan genes in pseudomonas putida.three indole analogues, 5-methylindole, 5-fluoroindole, and 7-methylindole, and the tryptophan analogue 5-fluorotryptophan were found to inhibit the growth of wild-type pseudomonas putida. mutants resistant to these analogues were obtained. some of the 5-fluoroindole- and 5-fluorotryptophan-resistant strains exhibit an abnormality in the regulation of certain trp genes. these strains excrete anthranilate when grown in minimal medium in the presence or absence of the inhibitor. in these strains, ...19715573729
induction specificity and catabolite repression of the early enzymes in camphor degradation by pseudomonas putida.the ability of bornane and substituted bornanes to induce the early enzymes for d(+)-camphor degradation and control of these enzymes by catabolite repression were studied in a strain of a pseudomonas putida. bornane and 20 substituted bornane compounds showed induction. of these 21 compounds, bornane and 8 of the substituted bornanes provided induction without supporting growth. oxygen, but not nitrogen, enhanced the inductive potency of the unsubstituted bornane ring. all bornanedione isomers ...19715573731
the metabolism of nicotinic acid. i. purification and properties of 2,5-dihydroxypyridine oxygenase from pseudomonas putida n-9. 19715578917
photoactivation of urocanase in pseudomonas putida. purification of inactive enzyme. 19715580658
2'-o-methyladenosine 5'-triphosphate. a substrate for deoxyribonucleic acid dependent ribonucleic acid polymerase of pseudomonas putida. 19714935104
pseudomonas putida tryptophan synthetase: partial sequence of the subunit.there is 50% identity in the sequences of the first 50 residues of the alpha chains of escherichia coli and pseudomonas putida. no deletions or additions of residues are found in this region, except for the n-terminal methionine residue which is missing in the polypeptide isolated from p. putida. most of the residues which differ are chemically dissimilar, and half of them are specified by codons which differ by more than a single base. the two residues known by mutational analysis to be essenti ...19714941557
pseudomonas putida tryptophan synthetase.the two protein components of pseudomonas putida tryptophan synthetase have been purified to homogeneity. although there is general similarity between the pseudomonas enzyme and that of the enteric bacteria, many differences were found. components from escherichia coli and p. putida do not stimulate each other enzymatically, and the enzymes differ in their response to monovalent cations. serine deamination occurs best with the intact enzyme of p. putida, not with the beta(2) subunit alone as in ...19714941565
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